阅读说明：本技术 用于原位合成dna阵列的羟烷基化聚丙烯酰胺表面涂层 (Hydroxyalkylated polyacrylamide surface coatings for in situ synthesis of DNA arrays ) 是由 格伦·麦克加尔 于 2018-03-06 设计创作，主要内容包括：本发明公开涉及通过在基底表面共价结合羟烷基化聚丙烯酰胺薄膜的方法衍生化该基底,并将该基底作为合成生物分子阵列的平台的一些过程和方法。这些方法也可以用于制备用于生物分子阵列原位固相合成的基底表面。(The present disclosure relates to processes and methods for derivatizing a substrate by covalently bonding a hydroxyalkylated polyacrylamide film to the surface of the substrate, and using the substrate as a platform for the synthesis of biomolecule arrays. These methods can also be used to prepare substrate surfaces for in situ solid phase synthesis of biomolecule arrays.)

1. A solid support comprising a surface covalently bound polymer comprising a compound of formula I:

wherein R is¹Can be independently selected from the following groups:

provided that there is at least one R¹Is that

T¹May be free of radicals, H, C₁-C₆Alkyl, or a first initiator residue;

T²may be free of radicals, H, C₁-C₆Alkyl, or a second initiator residue;

R²independently is H, -CH₃or-CH₂OCH₃；

R³And R⁴At each occurrence, independently is H or-CH₃；

The Capture Probe (Capture Probe) comprises at least one molecule selected from the group consisting of: peptides, proteins, glycosylated proteins, glycoconjugates, aptamers, sugars, polynucleotides, oligonucleotides and polypeptides;

x is an independent integer between 1 and 20;

y is an independent integer from 1 to 20;

z is an independent integer between 2 and 200;

p is an independent integer between 0 and 20;

q is an independent integer between 0 and 20;

a is an integer of 1 to 5;

b is an integer of 0 to 10;

c is an integer from 1 to 5; and is

d is an integer of 0 to 10.

2. The solid support of claim 1, further comprising a surface comprising a plurality of amino groups covalently attached to the surface, thereby allowing covalent attachment of the compound of formula I to at least a portion of the plurality of amino groups by way of an amide bond.

3. The solid support of claim 1, wherein p is an independent integer from 1 to 20 and q is an independent integer from 1 to 20.

4. The solid support of claim 1, wherein R¹May be independently selected from the following groups:

5. the solid support of claim 4, wherein the capture probe is an oligonucleotide.

6. The solid support of claim 4, wherein the capture probe is a DNA molecule.

7. The solid support of claim 2, wherein the surface is a glass or polymer substrate.

8. The solid support of claim 7, wherein the polymer substrate is at least one selected from the group consisting of: acrylonitrile-butadiene-styrene, cyclic olefin polymers, cyclic olefin copolymers, polymethyl methacrylate, polycarbonate, polystyrene, polyvinyl chloride, polyamide, polyethylene terephthalate, polytetrafluoro polyurethane, polyoxymethylene, thermoplastic elastomers, polyimide, polyether-ketone, polylactic acid, and polymethyl ether.

9. A solid support comprising a surface covalently bound polymer comprising a compound of formula I:

wherein R is¹Independently selected from the group consisting of:

T¹may be free of radicals, H, C₁-C₆Alkyl, or a first initiator residue;

T²may be free of radicals, H, C₁-C₆Alkyl, or a second initiator residue;

R²independently is H, -CH₃or-CH₂OCH₃；

R³And R⁴At each occurrence, independently is H or-CH₃；

x is an independent integer between 1 and 20;

y is an independent integer between 1 and 20;

z is an independent integer between 2 and 200;

p is an independent integer between 0 and 20;

q is an independent integer between 0 and 20;

a is an integer of 1 to 5;

b is an integer of 0 to 10;

c is an integer from 1 to 5; and is

d is an integer of 0 to 10.

10. The solid support of claim 9, further comprising a surface comprising a plurality of amino groups covalently attached to the surface, thereby allowing covalent attachment of the compound of formula I to at least a portion of the plurality of amino groups by way of an amide bond.

11. The solid support of claim 9, wherein p is an independent integer from 1 to 20 and q is an independent integer from 1 to 20.

12. The solid support of claim 9, wherein R¹May be independently selected from the following groups:

13. the solid support of claim 9, wherein R¹Is that

14. The solid support of claim 9, wherein R¹May be independently selected from the following groups:

15. the solid support of claim 10, wherein the surface is a glass or polymer substrate.

16. The solid support of claim 15, wherein the polymer substrate is at least one selected from the group consisting of: acrylonitrile-butadiene-styrene, cyclic olefin polymers, cyclic olefin copolymers, polymethyl methacrylate, polycarbonate, polystyrene, polyvinyl chloride, polyamide, polyethylene terephthalate, polytetrafluoro polyurethane, polyoxymethylene, thermoplastic elastomers, polyimide, polyether-ketone, polylactic acid, and polymethyl ether.

17. A method of derivatizing a substrate surface comprising:

(a) providing a substrate having a surface comprising a plurality of first amino groups;

(b) reacting a first plurality of reactive groups of a first reagent with a set of the plurality of first amino groups, thereby forming a surface-covalently bound film; and

(c) reacting the second plurality of reactive groups of the first reagent with a second reagent comprising a second amino and hydroxyalkyl functional group to form a hydroxyalkylated surface-bound film.

18. The process of claim 17, wherein the first amino group in (a) is a primary amine.

19. The method of claim 17, further comprising, prior to (a), treating the surface of the substrate with an aminoalkyl trialkoxysilane, an ammonia plasma, or RF plasma deposition.

20. The method of claim 17, wherein the first agent in (b) is an amine-reactive acrylate polymer or an amine-reactive acrylate-co-acrylamide copolymer.

21. The method of claim 20, wherein the amine-reactive acrylate polymer is a compound according to formula II:

wherein X is an amine reactive center independently selected from the group consisting of:

T¹may be free of radicals, H, C₁-C₆Alkyl, or a first initiator residue;

T²may be free of radicals, H, C₁-C₆Alkyl, or a second initiator residue; and

m is an integer of 2 to 800.

22. The method of claim 20, wherein the acrylate-co-acrylamide copolymer is a compound according to formula III:

wherein X is an amine reactive center, and at each occurrence, X is independently selected from:

R³and R⁴At each occurrence, independently is H or-CH₃；

T¹May be free of radicals, H, C₁-C₆Alkyl, or a first initiator residue;

T²may be free of radicals, H, C₁-C₆Alkyl, or a second initiator residue;

at each occurrence, m is independently an integer from 1 to 20;

at each occurrence, n is independently an integer from 1 to 20; and

m is an integer of 2 to 400.

23. The method of claim 17, wherein the reactive group is-c (o) X, and X is independently selected from the group consisting of:

24. the method of claim 17, further comprising reacting in (c) a third plurality of reactive groups with a third reagent comprising a third amino group instead of a hydroxyl group.

25. The method of claim 17, further comprising (d) reacting the surface-bound hydroxyalkylated membrane with a fourth reagent to synthesize an oligonucleotide array.

26. The method of claim 25, wherein the synthesizing in (d) comprises inkjet synthesis or photolithographic synthesis.

27. The method of claim 25, wherein the reacting in (d) comprises alkylating the hydroxyalkyl group with an oligonucleotide reagent.

28. The method of claim 17, wherein the first agent has a molecular weight of about 5,000 to about 200,000.