阅读说明：本技术 一种防治黏虫的新药及其使用方法 (Novel medicine for preventing and treating armyworm and application method thereof ) 是由 安世恒 杜孟芳 张云辉 刘晓光 魏纪珍 于 2019-10-29 设计创作，主要内容包括：本发明属于害虫防治的毒理活性领域,特别是指一种防治黏虫的新药及其使用方法。一种防治黏虫的新药,所述新药为环孢素A。所述黏虫指2龄黏虫。利用新药防治黏虫的方法,步骤为,将新药与人工饲料混合饲喂黏虫。本发明利用环孢素A混合黏虫人工饲料,通过设置不同剂量的环孢素A,检测7天后其对黏虫的防治效果。评价环孢素A的防治效果,确定环孢素A防治剂量。为防治黏虫的提供备用的杀虫剂或新药设计提供新的活性官能团。(The invention belongs to the field of toxicological activity of pest control, and particularly relates to a novel drug for pest control and a use method thereof. A new medicine for preventing and treating armyworm is cyclosporine A. The myxozoa refer to 2-year-old myxozoa. The method for preventing and treating the mythimna separata by using the new medicament comprises the step of mixing the new medicament with the artificial feed to feed the mythimna separata. According to the invention, the cyclosporine A is mixed with the myxobolus artificial feed, and the control effect of the cyclosporine A on the myxobolus is detected after 7 days by setting different doses of the cyclosporine A. Evaluating the control effect of the cyclosporin A, and determining the control dose of the cyclosporin A. Provides a new active functional group for providing a spare pesticide or a new drug design for preventing and controlling armyworms.)

1. A new drug for preventing and treating armyworm, which is characterized in that: the new drug is cyclosporine A.

2. The novel drug for controlling mythimna as claimed in claim 1, wherein: the myxozoa refer to 2-year-old myxozoa.

3. The method for controlling mythimna separata using the new drug as described in claim 1 or 2, characterized by the steps of: the new medicine is mixed with artificial feed to feed armyworm.

4. The method for controlling mythimna as claimed in claim 3, which comprises the steps of: the new medicine is used at the concentration of 50-150 muM.

5. The method for controlling mythimna as claimed in claim 3, which comprises the steps of: the lethal concentration of the new drug for preventing and treating 2-year myxozoa is 150 mu M.

6. The method for controlling mythimna as claimed in claim 3, which comprises the steps of: when the new medicine is used for preventing and treating 2-year myxozoa, the concentration of the mortality rate with the prevention effect reaching 70 percent is 100 mu M when the myxozoa are dead after being taken as being less than 3 years, and the concentration of the mortality rate with the prevention effect reaching more than 90 percent is 150 mu M.

7. The method for controlling mythimna as claimed in claim 3, which comprises the steps of: when the new medicine is used for preventing and treating 2-year myxozoa, the concentration of the mortality rate with the prevention effect reaching 100% is 100 mu M when the myxozoa with the age of less than 4 years are considered to die.

Technical Field

The invention belongs to the field of toxicological activity of pest control, and particularly relates to a novel drug for pest control and a use method thereof.

Background

Mythimna (A)Mythimna separata(Walker), belonging to the family of the order Lepidoptera and the family of the family SpodopteraThe insect pests are called as secatella vulgaris and armyworm, commonly called as confetti insect and wheat silkworm, and are the heterophagy, migratory and intermittent explosive pests which mainly use grain crops such as wheat, corn, sorghum, rice and the like and pasture. Myxozoon larvae are 6 years old in total, and 4-6 years old are binge eating period, which is the main stage of damage. Can harm more than 16 kinds of 104 plants in 16 families, especially the plants which like to eat gramineae. Except for the northwest local area, the other areas are distributed. When the armyworm is outbreak, the armyworm can eat the leaves of the crops, and the growth of the crops is seriously damaged. In recent years, armyworms often explode greatly in various places, and bring great damage to agricultural production, particularly the yield of corn.

At present, chemical control is mainly adopted for preventing and controlling armyworms. The application of common chemical pesticides, such as pyrethroid and organophosphorus pesticides, has serious light residue, is not environment-friendly and has increasingly prominent resistance problem. Therefore, in order to realize green, pollution-free and efficient prevention and control of corn armyworm, a novel pesticide or active molecule which is safe, reliable, low in toxicity and efficient needs to be found.

Therefore, some new chemical functional groups or novel pesticides are urgently needed, and the resistance can be treated, and the effect of treating armyworm can be achieved with high efficiency and low toxicity.

Disclosure of Invention

Aiming at the problem that novel pesticides and active molecules for preventing and controlling armyworms are lacked at present, the invention discovers that cyclosporine A has good insecticidal and inhibitory activity on the armyworms. The invention provides a new medicine for preventing and treating armyworm and a use method thereof.

The technical scheme of the invention is realized as follows:

a new medicine for preventing and treating armyworm is cyclosporine A.

The myxozoa refer to 2-year-old myxozoa.

The method for preventing and controlling armyworm by using the new medicine comprises the following steps: the new medicine is mixed with artificial feed and fed to mythimna separata.

The new medicine is used at the concentration of 50-150 muM.

The lethal concentration of the new drug for preventing and treating 2-year myxozoa is 150 mu M.

When the new medicine is used for preventing and treating 2-year myxozoa, the concentration of the mortality rate with the prevention effect reaching 70 percent is 100 mu M when the myxozoa are dead after being taken as being less than 3 years, and the concentration of the mortality rate with the prevention effect reaching more than 90 percent is 150 mu M.

When the new medicine is used for preventing and treating 2-year myxozoa, the concentration of the mortality rate with the prevention effect reaching 100% is 100 mu M when the myxozoa with the age of less than 4 years are considered to die.

The invention has the following beneficial effects:

1. according to the invention, the cyclosporine A is mixed with the myxobolus artificial feed, and the control effect of the cyclosporine A on the myxobolus is detected after 7 days by setting different doses of the cyclosporine A. Evaluating the control effect of the cyclosporin A, and determining the control dose of the cyclosporin A. Provides a new active functional group for providing a spare pesticide or a new drug design for preventing and controlling armyworms.

2. The inventor finds that the biological source medicament has good insecticidal activity on agricultural pests such as armyworm through experiments. Cyclosporin A acts on armyworm, inhibits the expression of dephosphorylation enzyme, and further influences the expression and activity of antibacterial peptide and detoxification enzyme in insect bodies. The cyclosporin a itself or a compound having a chemical structure similar thereto is likely to be a novel insecticide or may be used as a novel insecticidal functional group to be applied to the development and utilization of a novel pesticide.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a graph showing the lethality of cyclosporin A at various doses to myxomatous 2-instar larvae; data in the figure are mean ± sem.

FIG. 2 is a graph showing the lethality of cyclosporin A at various doses to 2 instar larvae of myxophora (deaths were considered when instars were less than 3 instars); data in the figure are mean ± sem.

FIG. 3 is a graph showing the lethality of cyclosporin A at various doses to 2 instar larvae of myxozoa (larvae younger than 4 instar are considered dead); data in the figure are mean ± sem.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments of the present invention, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive effort based on the embodiments of the present invention, are within the scope of the present invention.