阅读说明：本技术 一种含有Acynonapyr的杀螨组合物、制剂剂型及其应用 (Acaricidal composition containing Acynonapyr, preparation formulation and application thereof ) 是由 周大伟 郑敬敏 马强 刘欢 于 2019-10-09 设计创作，主要内容包括：本发明公开了一种含有Acynonapyr的杀螨组合物、制剂剂型及应用,属于杀螨剂领域；所述杀螨组合物的有效成分包括Acynonapyr和联苯肼酯；两者的质量比为1:5～5:1,优选为1:5～1:1,更优选为1:3～1:2；按质量百分比计,所述杀螨组合物的含量为1%～50%；本组合物可配制成农业上允许的悬浮剂、可分散油悬浮剂、水分散粒剂、乳油、微乳剂。本发明组分合理,对农业害螨具有较好的防治效果,与现有单一制剂相比,增效作用明显,能够降低农药使用量与成本,减少环境的污染,延长农药使用寿命。(The invention discloses an acaricidal composition containing Acynonapyr, a preparation formulation and application, belonging to the field of acaricides; the effective components of the acaricidal composition comprise Acynonapyr and bifenazate; the mass ratio of the two is 1: 5-5: 1, preferably 1: 5-1: 1, and more preferably 1: 3-1: 2; the content of the acaricidal composition is 1-50% by mass; the composition can be prepared into agriculturally acceptable suspending agents, dispersible oil suspending agents, water dispersible granules, missible oil and micro-emulsions. The invention has reasonable components, has better control effect on agricultural pest mites, has obvious synergistic effect compared with the prior single preparation, can reduce the usage amount and cost of pesticides, reduce environmental pollution and prolong the service life of the pesticides.)

1. The acaricidal composition containing Acynonapyr is characterized in that effective active ingredients comprise Acynonapyr and bifenazate, wherein the mass ratio of the Acynonapyr to the bifenazate is 1: 5-5: 1.

2. The Acynonapyr-containing acaricidal composition according to claim 1, wherein the mass ratio of the Acynonapyr to the bifenazate is 1: 5-1: 1, preferably 1: 3-1: 2.

3. An acaricidal composition preparation containing Acynonapyr, which is characterized by comprising an acaricidal active ingredient and an auxiliary agent, wherein the acaricidal active ingredient is the acaricidal composition in claim 1 or 2, and the acaricidal composition is contained in an amount of 1-50% by mass.

4. The Acynonapyr-containing acaricidal composition formulation according to claim 3, wherein the composition is in the form of a suspension, a dispersible oil suspension, water dispersible granules, emulsifiable concentrate or microemulsion.

5. Use of the acaricidal composition according to claim 1 containing acyclonapyr and bifenazate for controlling agricultural mites.

6. The use of claim 5, wherein the pest mites are one or more of Tetranychus cinnabarinus, Tetranychus crataegi, Panonychus ulmi, Panonychus citri and Tetranychus urticae.

Technical Field

The invention belongs to the field of acaricide compound pesticides, and relates to an acaricide composition containing Acynonapyr as an effective active ingredient, a preparation formulation and application thereof.

Background

Acytonapyr, chemical name: (3-endo) -3- [ 2-propoxy-4- (trifluoromethyl) phenoxy ] -9- [ [5- (trifluoromethyl) -2-pyridinyl ] oxy ] -9-azabicyclo [3.3.1] nonane. The structural formula is shown as formula 1. The acaricide is an acaricide with cyclic amine skeleton developed by Nippon Caoda corporation, acts on inhibitory glutamate receptors, interferes with the neurotransmission of harmful mites, causes the dyskinesia of the harmful mites, and finally kills the harmful mites.

Formula 1

Bifenazate, english name: bifenazate, chemical name: 3- (4-Methoxybiphenyl-3-yl) hydrazinoformic acid isopropyl ester. The structural formula is shown as formula 2. The acaricide is a selective leaf surface spray acaricide, is non-systemic, is effective to all life stages of mites, has ovicidal activity and quick knockdown activity to adult mites, mainly acts on gamma-aminobutyric acid (GABA) receptors of central nervous conduction systems of mites, has low toxicity, long lasting period and no cross resistance with the existing commercial acaricide, is environment-friendly, and can be used for preventing and treating red spider mites, two-spotted spider mites and McDaniel mites on apples and grapes and two-spotted spider mites and Lewis mites of ornamental plants.

Formula 2

The harmful mites are important harmful arthropods on crops, fruit trees, vegetables, woods and flowers, and agricultural harmful mites in China mainly comprise tetranychidae, gall mites and tarsal mites, wherein the tetranychidae are the largest groups. When the pest mites are harmful plants, the normal physiological functions of the plants are damaged, fallen leaves, fallen buds and fallen fruits are caused, the tree vigor is weakened, the yield is reduced, and some pest mites can also be used as transmission carriers of plant diseases. In China, harmful mites seriously harming apples mainly comprise: panonychus ulmi, Tetranychus viennensis; harmful mites harmful to citrus are mainly: panonychus citri, tetranychus citri, rust mites of citrus, gall mites of citrus; the harmful mites harmful to vegetables are as long as: tetranychus zeylanicus, tetranychus urticae, tetranychus cinnabarinus; harmful mites harmful to crops mainly comprise: ornithogalus stringonii, Tarsonemus tarsi, Melilotus mairei, Tetranychus Zeylanica, Tetranychus urticae and Tetranychus cinnabarinus.

Disclosure of Invention

The invention aims to provide the acaricidal composition which has reasonable components, remarkable synergistic effect, good acaricidal effect, low medication cost and safety to crops. Wherein the active ingredients comprise Acynonapyr and bifenazate.

The invention also aims to provide preparation formulations containing the acaricidal composition, which comprise suspending agents, dispersible oil suspending agents, water dispersible granules, missible oil and microemulsion.

The invention also aims to provide application of the composition in preventing and controlling harmful mites.

In order to achieve the purpose, the technical scheme of the invention is as follows:

an acaricidal composition containing Acynonapyr, which is characterized in that,

a first active ingredient: acynonapyr;

the second active ingredient: bifenazate;

the weight ratio of the first active ingredient to the second active ingredient is 1: 5-5: 1, preferably 1: 5-1: 1, and more preferably 1: 3-1: 2.

The acaricidal composition preparation containing Acynonapyr is characterized in that the sum of the content of the compositions is 1-50% of the total amount of the preparation in percentage by mass.

The acaricidal composition preparation formulation containing Acynonapyr can be prepared into suspending agents, dispersible oil suspending agents, water dispersible granules, missible oil and micro-emulsion according to the method known by the technical personnel in the technical field.

For the suspending agents, usable are: dispersing agent such as one or more of polycarboxylate, lignosulfonate, alkylnaphthalene formaldehyde condensate sulfonate, alkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether sulfonate, phenethyl phenol polyoxyethylene ether phosphate, alkyl polyoxyethylene ether sulfonate, polyoxyethylene polyoxypropylene ether block copolymer, nekal, dodecyl polyoxyethylene ether phosphate; wetting agents such as one or more of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, phenethylphenol polyoxyethylene ether phosphate ester, alkyl sulfate, alkyl sulfonate, alkyl naphthalene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, triphenylethylphenol polyoxypropylene polyoxyethylene block polymer; thickening agent such as xanthan gum, polyvinyl alcohol, bentonite, magnesium aluminum silicate, carboxymethyl cellulose, carboxyethyl cellulose, and methyl cellulose; preservatives such as one or more of formaldehyde, benzoic acid, sodium benzoate, potassium sorbate, isothiazolinone; defoaming agents such as silicone oil, silicone compound, C10-20 saturated fatty acid compound, C8-10 fatty alcohol, hexanol, butanol, octanol; antifreeze agent such as one or more of ethylene glycol, propylene glycol, glycerol, polyethylene glycol, sorbitol, urea, and inorganic salt; the water is deionized water.

For dispersible oil suspensions, the adjuvants that can be used are: dispersing agent such as one or more of polycarboxylate, lignosulfonate, alkylnaphthalene sulfonate (dispersant NNO), TERSPERSE 2020 (product of Huntsman corporation, U.S. alkyl naphthalene sulfonate); the emulsifier is selected from BY (polyoxyethylene castor oil) series emulsifier (BY-110, BY-125, BY-140), agricultural milk 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), agricultural milk 2201, span-60 # (common name: sorbitan monostearate), Tween-60 # (common name: sorbitan monostearate polyoxyethylene ether), agricultural milk 1601# (common name: phenethylphenol polyoxyethylene polyoxypropylene ether), and TERSPERSE 4894 (produced BY Henshimei corporation, USA); wetting agents such as one or more of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, naphthalene sulfonate, TERSPERSE 2500 (available from henseme, usa); thickening agent such as one or more of white carbon black, polyvinyl alcohol, bentonite, and magnesium aluminum silicate; antifreeze agents such as one or more of ethylene glycol, propylene glycol, glycerol, urea, inorganic salts such as sodium chloride; dispersing medium such as one or more of soybean oil, oleum Rapae, wheat oil, methyl oleate, diesel oil, engine oil, and mineral oil.

For water dispersible granules, the following additives can be used: dispersants such as one or more of polycarboxylates (TERSPERSE 2700, T36, GY-D06, etc.), lignosulfonates, alkylnaphthalene sulfonates, and the like; wetting agents such as one or more of alkyl sulfates, alkyl sulfonates, naphthalene sulfonates, and the like; disintegrating agent such as one or more of ammonium sulfate, sodium sulfate, polyvinylpyrrolidone, starch and its derivatives, bentonite, etc.; binder such as one or more of starch, glucose, polyvinyl alcohol, polyethylene glycol, sodium carboxymethylcellulose, sucrose, etc.; the filler is one or more of diatomite, kaolin, white carbon black, light calcium carbonate, talcum powder, attapulgite, pottery clay and the like.

For emulsifiable concentrates, the auxiliaries which can be used are: emulsifiers such as calcium dodecylbenzene sulfonate (Nongru 500 #), Nongru 700#, Nongru 2201, span-60 #, emulsifier T-60 (common name: sorbitan monostearate polyoxyethylene ether), TX-10 (common name: octylphenol polyoxyethylene (10) ether), Nongru 1601#, Nongru 600#, Nongru 400 #; solvents such as xylene, mineral spirits (S-150, S-180, S-200), toluene, biodiesel, methyl esterified vegetable oils, N-methyl pyrrolidone; co-solvents such as ethyl acetate, methanol, dimethylformamide, cyclohexanone, acetone, methyl ethyl ketone; stabilizers such as triphenyl phosphite, epichlorohydrin, acetic anhydride.

For microemulsions, the auxiliaries which can be used are: emulsifier such as one or more of Nongru 500# (calcium dodecylbenzenesulfonate), Nongru 700#, Nongru 2201, span-60 #, Tween 60- #, TX-10, Nongru 1601 (phenylethylphenol polyoxyethylene polyoxypropylene ether), Nongru 600#, Nongru 400#, etc.; auxiliary emulsifier such as one or more of methanol, isopropanol, n-butanol, and ethanol; solvents such as one or more of cyclohexanone, N-methylpyrrolidone, xylene, toluene, mineral spirits (trade names: S-150, S-180, S-200), etc.; one or more of stabilizers such as triphenyl phosphite, epichlorohydrin, and the like; the water is deionized water.

The application of the invention to agricultural pest mites is listed as the following test objects:

the Panonychus ulmi Karsch, Latin, is known by the chemical name:Panonychus ulmibelonging to the order Acarina, Tetranychidae. Can be used for parasitizing on fruit trees such as apple, pear, peach, plum, apricot, hawthorn, etc., and oriental cherry and rose. The main hazard modes are as follows: panonychus ulmi (L.) MerrThe young mites of the mite group, if the mites, are mostly male adult mites move and feed on the leaf backs, while the female adult mites move on the leaf surfaces, absorb the chlorophyll particles, and the chlorophyll contained in the leaves is reduced, so that the photosynthesis is inhibited, the leaves are scorched, and the leaves fall in advance.

Panonychus citri, a scientific name for Latin:Panonchus citri，belonging to the order Acarina, Tetranychidae. Is one of the most serious pests commonly occurring in citrus producing areas in China, and is called as the first pest of citrus vividly. The main hazard modes are as follows: adult, young and young mites prick the epidermis of host leaves, fruits and twigs by mouth to absorb juice, countless grey-white small spots appear on the damaged leaf surface, and the whole leaves lose green to turn grey-white when the disease is serious, so that a large amount of fallen leaves, fallen flowers and fallen fruits are caused, and the yield is seriously influenced.

Tetranychus urticae, the academic name of latin:Tetranychus urticae，belonging to the order Acarina, Tetranychidae. Can be parasitized on various fruit trees such as apple, peach, pear, apricot, plum and the like and weeds such as chenopodium album, amaranth and the like. The main hazard modes are as follows: parasitizing on the back of plant leaves to take food, piercing cells and sucking juice, wherein pale spots appear on the damaged leaves from two sides of a main vein close to a petiole, the leaves can become grey white and dark brown along with the aggravation of harm, the normal operation of photosynthesis is inhibited, and the serious leaves are scorched to fall off in advance, so that the tree vigor, the fruit quality and the fruit yield are seriously influenced.

Tetranychus viennensis, the scientific name of Latin is:Tetranychus viennensisbelonging to the order Acarina, Tetranychidae. Can be parasitized on various fruit trees such as pear, apple, peach, cherry, hawthorn, plum, etc. The main hazard modes are as follows: the juice is absorbed on the back of the leaves and young shoots of the leaves in various insect states, so that the leaves are withered and fall off gradually, and a large amount of flowers, leaves and fruits fall off when the harm is serious.

Tetranychus cinnabarinus, the scientific name of latin is:Tetranychus cinnabarinus，belonging to the order Acarina, Phyllanthus. The hosts mainly comprise: eggplant, hot pepper, watermelon, beans, shallot and amaranth. The main hazard modes are as follows: absorbing juice of stem and leaf of plant, reducing water content of affected part, showing green and white, dense pale speckles on leaf surface, curling and yellowing. In severe cases, yellow leaves, scorched leaves, curled leaves, fallen leaves, death and the like occur to plants.

The technical effects achieved are as follows:

compared with the existing single preparation, the active and acaricidal effects of the pesticide composition are not simple superposition of the activities of the components, but have obvious synergistic effect, so that the effects of reducing the dosage and the medication cost are achieved, and the pesticide composition meets the safety requirement of pesticide preparations.

Detailed Description

The control effect of blending Acynonapyr and bifenazate on tetranychus cinnabarinus is demonstrated by indoor toxicity test.

The indoor toxicity determination method refers to part 12 of pesticide of NY/T1154.12-2008 pesticide indoor bioassay test criteria: tetranychus slide soaking method.

Preparation of test materials: the mites to be tested are selected from female adult mites collected in the field and bred indoors. Firstly, cutting a double-sided adhesive tape into 2cm long, sticking the double-sided adhesive tape to one end of a glass slide, then selecting female adult mites with consistent physiological states bred in a room, sticking the backs of the female adult mites to the double-sided adhesive, paying attention to no need of sticking mite feet, tentacles and mouthparts, placing each female adult mite with 30 heads into a container padded with wet sponge, covering the container with a cover, culturing at 25 +/-1 ℃, performing microscopic examination after 2 hours, removing dead and injured individuals if the dead and injured individuals exist, and then supplementing 30 heads.

Medicament treatment: placing the glass slide in the prepared liquid medicine, slightly shaking for 5s, taking out, then sucking off the redundant liquid medicine by using absorbent paper, placing the glass slide in a white porcelain plate padded with wet sponge, and covering the white porcelain plate with a thin film with good light transmittance. The porcelain plate containing the treated test mites is kept and observed under the conditions that the temperature is 25 +/-1 ℃ and the light cycle is L: D = (16, 8) h.

The death of the test mites was checked 48h after the treatment and the total and dead mite numbers were recorded separately. Repeating the treatment for 4 times, and setting the treatment of spraying clear water as a blank control. The test data is statistically processed by DPS software to obtain correlation coefficient, virulence regression equation, lethal middle concentration, etc., and the cotoxicity coefficient is obtained by Sun cloud Pepper method.

The joint virulence of the mixed preparation is expressed by adopting a Sunweier cotoxicity coefficient method:

the synergistic effect of the acaricidal composition on tetranychus cinnabarinus and tetranychus crataegi is evaluated by adopting a co-toxicity coefficient method, wherein the co-toxicity coefficient is more than 120 and is shown as the synergistic effect, the co-toxicity coefficient is between 80 and 120 and is shown as the addition effect, and the co-toxicity coefficient is less than 80 and is shown as the antagonism effect. The toxicity test results of the Acynonapyr and bifenazate mixed on tetranychus cinnabarinus are shown in table 1; toxicity test results of Acynonapyr and bifenazate mixed on tetranychus viennensis are shown in Table 2.

TABLE 1 toxicity test results of Acynonapyr and Bifenazate mixture against Tetranychus cinnabarinus

From table 1, it can be seen: when the Acynonapyr and the bifenazate are mixed according to the proportion of 1: 5-5: 1, the tetranychus cinnabarinus has a certain toxicity effect. When the ratio of the two is 1: 5-1: 1, the co-toxicity coefficient is larger than 120, the synergistic effect is shown on tetranychus cinnabarinus, and particularly when the ratio of Acynonapyr to bifenazate is 1:3, the co-toxicity coefficient is the largest, and the synergistic effect is most obvious.

Table 2 toxicity test results of Acynonapyr and bifenazate mixed on Tetranychus viennensis

From table 2, it can be seen: the Acynonapyr and the bifenazate are mixed according to the proportion of 1: 5-5: 1, the Acynonapyr and the bifenazate have certain toxicity to the tetranychus urticae koch, when the proportion of the Acynonapyr and the bifenazate is 1: 5-1: 1, the cotoxicity coefficient is more than 120, the Acynonapyr and the bifenazate have a synergistic effect on the tetranychus urticae koch, particularly when the proportion of the Acynonapyr and the bif: and 2, the co-toxicity coefficient is the largest, and the synergistic effect is the most obvious.

The present invention will be further described with reference to the following examples.

Formulation example 1

40 g of Acynonapyr, 120 g of bifenazate, 50 g of polyoxyethylene polyoxypropylene ether block copolymer, 50 g of dodecyl polyoxyethylene ether phosphate, 30 g of bentonite, 20 g of polyvinyl alcohol, 25 g of sorbitol, 8 g of sodium benzoate, 15 g of hexanol and deionized water are weighed to 1000 g by weight. Mixing the above raw materials, high-speed shearing and dispersing for 30min, and sanding with sand mill to obtain particle diameter D₉₀Less than 10 μm, a 16% Acynonapyr bifenazate suspension was prepared.

Formulation example 2

50 g of Acynonapyr, 150 g of bifenazate, 70 g of phenethyl phenol polyoxyethylene ether phosphate, 40 g of sodium lignosulfonate, 20 g of carboxyethyl cellulose, 30 g of glycerol, 5 g of sodium benzoate, 15 g of octanol are weighed, and deionized water is added to 1000 g of parts by weight. Mixing the above raw materials, high-speed shearing and dispersing for 30min, and sanding with sand mill to obtain particle diameter D₉₀Less than 10 μm, a 20% Acynonapyr bifenazate suspension was prepared.

Formulation example 3

40 g of Acynonapyr, 160 g of bifenazate, 50 g of polyoxyethylene polyoxypropylene ether block copolymer, 50 g of dodecyl polyoxyethylene ether phosphate, 30 g of bentonite, 20 g of polyvinyl alcohol, 25 g of sorbitol, 8 g of sodium benzoate, 15 g of hexanol and deionized water are weighed to 1000 g by weight. Mixing the above raw materials, high-speed shearing and dispersing for 30min, and sanding with sand mill to obtain particle diameter D₉₀Less than 10 μm, a 20% Acynonapyr bifenazate suspension was prepared.

Formulation example 4

50 g of Acynonapyr, 200 g of bifenazate, 70 g of phenethyl phenol polyoxyethylene ether phosphate, 40 g of sodium lignosulfonate, 20 g of carboxyethyl cellulose, 30 g of glycerol, 5 g of sodium benzoate, 15 g of octanol are weighed, and deionized water is added to 1000 g of parts by weight. On the upper partThe raw materials are mixed, high-speed sheared and dispersed for 30min, and are sanded to a particle size D by a sand mill₉₀Less than 10 μm, a 25% Acynonapyr bifenazate suspension was prepared.

Formulation example 5

40 g of Acynonapyr, 80 g of bifenazate, 50 g of polyoxyethylene polyoxypropylene ether block copolymer, 50 g of dodecyl polyoxyethylene ether phosphate, 30 g of bentonite, 20 g of polyvinyl alcohol, 25 g of sorbitol, 8 g of sodium benzoate, 15 g of hexanol and deionized water are weighed to 1000 g by weight. Mixing the above raw materials, high-speed shearing and dispersing for 30min, and sanding with sand mill to obtain particle diameter D₉₀Less than 10 μm, a 12% Acynonapyr bifenazate suspension was prepared.

Formulation example 6

50 g of Acynonapyr, 100 g of bifenazate, 70 g of phenethyl phenol polyoxyethylene ether phosphate, 40 g of sodium lignosulfonate, 20 g of carboxyethyl cellulose, 30 g of glycerol, 5 g of sodium benzoate, 15 g of octanol are weighed, and deionized water is added to 1000 g of parts by weight. Mixing the above raw materials, high-speed shearing and dispersing for 30min, and sanding with sand mill to obtain particle diameter D₉₀Less than 10 μm, a 15% Acynonapyr bifenazate suspension was prepared.

Formulation example 7

Weighing 40 g of Acynonapyr, 200 g of bifenazate, 60 g of phenethyl phenol polyoxyethylene ether phosphate, 30 g of polyoxyethylene polyoxypropylene block copolymer, 40 g of span-60 #, 20 g of agricultural milk 700#, 25 g of calcium dodecyl benzene sulfonate, 20 g of organic bentonite, 10 g of magnesium aluminum silicate, 15 g of BHT and rapeseed oil for supplementing 1000 g. The raw materials are mixed and ground by a sand mill until the particle size is less than 5 mu m, and the 24 percent Acynonapyr bifenazate dispersible oil suspending agent is prepared.

Formulation example 8

Weighing 50 g of Acynonapyr, 250 g of bifenazate, 50 g of pesticide emulsion 2201, 40 g of sodium lignosulfonate, 30 g of fatty alcohol-polyoxyethylene ether, 30 g of pesticide emulsion 1601#, 20 g of polyvinyl alcohol, 20 g of epichlorohydrin, and supplementing 1000 g of palm oil. The raw materials are mixed and ground by a sand mill until the particle size is less than 5 mu m, and the 30 percent Acynonapyr bifenazate dispersible oil suspending agent is prepared.

Formulation example 9

Weighing 40 g of Acynonapyr, 40 g of bifenazate, 60 g of phenethyl phenol polyoxyethylene ether phosphate, 30 g of polyoxyethylene polyoxypropylene block copolymer, 40 g of span-60 #, 20 g of agricultural milk 700#, 25 g of calcium dodecyl benzene sulfonate, 20 g of organic bentonite, 10 g of magnesium aluminum silicate, 15 g of BHT and rapeseed oil for supplementing 1000 g. The raw materials are mixed and ground by a sand mill until the particle size is less than 5 mu m, and then the 8 percent Acynonapyr bifenazate dispersible oil suspending agent is prepared.

Formulation example 10

Weighing 50 g of Acynonapyr, 50 g of bifenazate, 50 g of pesticide emulsion 2201, 40 g of sodium lignosulfonate, 30 g of fatty alcohol-polyoxyethylene ether, 30 g of pesticide emulsion 1601#, 20 g of polyvinyl alcohol, 20 g of epichlorohydrin, and supplementing 1000 g of palm oil. The raw materials are mixed and ground by a sand mill until the particle size is less than 5 mu m, and then the 10 percent Acynonapyr bifenazate dispersible oil suspending agent is prepared.

The following biological examples 1, 2, 3 demonstrate the field synergistic effect of the compositions. The test method and the calculation formula are as follows:

the test method comprises the following steps: the method is characterized in that cells are randomly arranged, cultivation conditions are uniform, 3 trees are planted in each cell, a blank contrast area is arranged outside a test cell, cross contamination is prevented, each treatment is repeated for 4 times, a 10-time hand-held magnifier is used for random sampling and investigation of leaves, and pesticide application is carried out when live mites exist in each visual field for 3-5 times on average.

The investigation method comprises the following steps: each district investigates 2 trees, 5 damaged leaves of the same season are marked in the east, west, south, north and middle five directions of the trees, and the number of active mites on 50 leaves is investigated in each district. And directly observing the front and back surfaces of the leaf by using a handheld magnifier, and counting the number of live mites. If the damage is light, the number of investigation blades can be increased. The number of active mites was recorded 3 days, 7 days, 12 days, 20 days, and 25 days after the treatment.

PTThe mite mouth decline rate in the medicament treatment area;CKthe rate of oral mite decline in the blank control area is shown.

Biological example 1: and (3) field pesticide effect test for controlling panonychus ulmi.

In 2018, 12 months in 5.8, formulation example 1 (16% of Acynonapyr bifenazate suspending agent), formulation example 3 (20% of Acynonapyr bifenazate suspending agent), formulation example 5 (12% of Acynonapyr bifenazate suspending agent), formulation example 7 (24% of Acynonapyr bifenazate dispersible oil suspending agent), and formulation example 9 (8% of Acynonapyr bifenazate dispersible oil suspending agent) are performed in Sophora arborvitae of Luochaena mala in Shanxi province, and the synergistic effect after compounding is verified by comparing the effects of the control agents 20% of Acynonapyr suspending agent and 43% of bifenazate suspending agent. The test results are shown in Table 3.

Table 3 Acynonapyr and bifenazate compounded field pesticide effect test result for controlling panonychus ulmi

As can be seen from Table 3, when the dosage of the active ingredients in the above preparation examples is 100mg/kg, the control effect on panonychus ulmi 3, 7, 12, 20 and 25 days after the application is higher than the control effects of 100mg/kg of 20% Acynonapyr suspending agent and 143mg/kg of 43% bifenazate suspending agent. The compounding of Acynonapyr and bifenazate has a synergistic effect. And all tested agents are safe to apple growth during the test period, have no phytotoxicity phenomenon and are harmless to natural enemies.

Biological example 2: and (3) field pesticide effect test for controlling panonychus citri.

Formulation example 2 (20% Acynonapyr bifenazate suspension), formulation example 4 (25% Acynonapyr bifenazate suspension), formulation example 6 (15% Acynonapyr bifenazate suspension), formulation example 8 (30% Acynonapyr bifenazate dispersible oil suspension), and formulation example 10 (10% Acynonapyr bifenazate dispersible oil suspension) were performed in Anshan Zhen (Yanshan town, Guinea city, Osmanthi, Guangxi province) of Guangxi of Kyowa of Guangxi nationality at 5/15/2018, and the synergistic effect after formulation was verified by comparing the effects of the control agents 20% Acynonapyr suspension and 43% bifenazate suspension. The test results are shown in Table 4.

Table 4 Acynonapyr and bifenazate compounded field pesticide effect test result for controlling panonychus citri

Further, as shown in table 4, when the dosage of the effective components in the above formulation examples is 100mg/kg, the control effect on tetranychus urticae is obviously higher than the control effect of 100mg/kg of 20% Acynonapyr suspending agent and 143mg/kg of 43% bifenazate suspending agent after the administration for 3, 7, 12, 20 and 25 days. The compounding of Acynonapyr and bifenazate has a synergistic effect. Safety survey during test shows that all the test agents are safe to the growth of citrus, do not have the phenomenon of phytotoxicity and are harmless to natural enemies.

Biological example 3: and (3) field pesticide effect test for preventing and treating tetranychus urticae.

In 2018, 5, 12 days in Shaanxi province, Sophora arborvitae, Luochuan city, Yan city, in preparation example 2 (20% Acynonapyr bifenazate suspending agent), preparation example 4 (25% Acynonapyr bifenazate suspending agent), preparation example 6 (15% Acynonapyr bifenazate suspending agent), preparation example 8 (30% Acynonapyr bifenazate dispersible oil suspending agent), and preparation example 10 (10% Acynonapyr bifenazate dispersible oil suspending agent) are subjected to field tests for controlling two-spotted spider mites on apple trees, and the synergistic effect after compounding is verified by comparing the effects of the 20% Acynonapyr suspending agent and 43% bifenazate suspending agent with the control agents. The test results are shown in Table 5.

TABLE 5 field efficacy test results of Acynonapyr and Bifenazate compounded for controlling Tetranychus urticae

From table 5, when the dosage of the effective components in the preparation examples is 100mg/kg, the control effect on tetranychus urticae 3, 7, 12, 20 and 25 days after the preparation is obviously higher than the control effects of 100mg/kg of 20% Acynonapyr suspending agent and 143mg/kg of 43% bifenazate suspending agent, which shows that the Acynonapyr and bifenazate have obvious synergistic effect after being compounded. Safety survey during the test period shows that all the test agents are safe to apple growth, do not have the phenomenon of phytotoxicity and are harmless to natural enemies.

In conclusion, the Acynonapyr and the bifenazate with different action mechanisms are compounded, so that the compound has the following advantages and beneficial effects: 1. by using the compound scheme, the composition has obvious synergistic effect on pest mites, and the control effect is obviously improved; 2. the dosage and the frequency of medication are reduced, and the prevention and treatment cost is reduced; 3. the pesticide resistance of the pests is delayed, the safety of the protected objects is improved, and the pesticide is an ideal pesticide for preventing and controlling harmful mites, so the pesticide has wide application prospect in production.