阅读说明：本技术 一种纯植物提取的驱蚊液及其制备方法 (Mosquito repellent liquid extracted from pure plants and preparation method thereof ) 是由 陆榕 张校铭 王玉珏 于 2018-06-27 设计创作，主要内容包括：本发明属于驱蚊制剂领域,特别涉及一种纯植物提取的驱蚊液及其制备方法。本发明所述的纯植物提取的驱蚊液包括以下体积份数的原料制成：红豆杉浸提液2～4份；青蒿浸提液3～4份；苦参浸提液1～2份；蛇床子浸提液1～2份；去离子水87份。制备步骤包括初步提取、纯化、混合、微囊的制备；本发明由纯植物提取液混合加工制成,不含各种驱蚊酯和冰片等有毒化学成分,适用于婴幼儿、孕妇和易过敏人群；配方合理,产品驱蚊的同时还具备杀菌止痒的作用；驱蚊效果好,持续时间长。(The invention belongs to the field of mosquito repellent preparations, and particularly relates to a pure plant-extracted mosquito repellent liquid and a preparation method thereof. The mosquito repellent liquid extracted from pure plants is prepared from the following raw materials in parts by volume: 2-4 parts of a Chinese yew leaching solution; 3-4 parts of a sweet wormwood herb leaching solution; 1-2 parts of sophora flavescens leaching liquor; 1-2 parts of fructus cnidii leaching liquor; 87 parts of deionized water. The preparation steps comprise primary extraction, purification, mixing and microcapsule preparation; the mosquito repellent liquid is prepared by mixing and processing pure plant extract, does not contain various toxic chemical components such as mosquito repellent ester, borneol and the like, and is suitable for infants, pregnant women and allergy-prone people; the formula is reasonable, and the product has the effects of killing bacteria and relieving itching while repelling mosquitoes; the mosquito repellent effect is good, and the duration is long.)

1. The mosquito repellent liquid extracted from pure plants is characterized by being prepared from the following raw materials in parts by volume:

2-4 parts of a Chinese yew leaching solution;

3-4 parts of a sweet wormwood herb leaching solution;

1-2 parts of sophora flavescens leaching liquor;

1-2 parts of fructus cnidii leaching liquor;

87 parts of deionized water.

2. The pure plant extracted mosquito repellent liquid according to claim 1, which is prepared by mixing the following components in parts by volume:

4 parts of a Chinese yew leaching solution;

4 parts of sweet wormwood leaching liquor;

2 parts of sophora flavescens leaching liquor;

2 parts of fructus cnidii leaching liquor;

87 parts of deionized water.

3. A method for preparing a mosquito repellent liquid extracted from pure plants according to claim 1 or 2, which is characterized by comprising the following steps of primary extraction:

primarily extracting the taxus chinensis: weighing dried taxus chinensis barks, adding ethanol, sealing, and leaching for 1-7 days in a shady, ventilated and dark place to obtain a primary taxus chinensis leaching solution;

primary extraction of sweet wormwood herb: weighing dried over-ground plants of the sweet wormwood herb, adding ethanol, sealing, placing in a shady, ventilated and dark place, and leaching for 1-7 days to obtain a primary sweet wormwood herb leaching solution;

primarily extracting the radix sophorae flavescentis: weighing dried radix sophorae flavescentis, adding ethanol, sealing, placing in a shady, ventilated and dark place, and leaching for 1-7 days to obtain a primary radix sophorae flavescentis leaching solution;

primary extraction of fructus cnidii: weighing dried fructus cnidii mature fruits, adding ethanol, sealing, and leaching in a shady, ventilated and dark place for 1-7 days to obtain a fructus cnidii primary leaching solution.

4. The method for preparing the mosquito repellent liquid extracted from the pure plants according to claim 3, which is characterized by further comprising the following steps of purification:

purifying a primary Chinese yew leaching solution: filtering the taxus chinensis primary leaching solution, adding deionized water with the same volume, refrigerating, stirring, filtering, keeping the temperature of filtrate at 30-80 ℃, adding active carbon with the volume fraction of 0.1-0.5%, stirring for decolorization, filtering, and performing rotary evaporation until the ethanol concentration is lower than 25wt.% to obtain a taxus chinensis leaching solution;

and (3) purifying a sweet wormwood primary leaching solution: filtering the sweet wormwood primary leaching solution, adding deionized water with the same volume, refrigerating, stirring, filtering, keeping the temperature of filtrate at 30-80 ℃, adding active carbon with the volume fraction of 0.1-0.5%, stirring, decoloring, filtering, and performing rotary evaporation until the ethanol concentration is lower than 25 wt%, thereby obtaining sweet wormwood leaching solution;

and (3) purifying a fructus cnidii primary leaching solution: filtering the fructus cnidii primary leaching solution, adding deionized water with the same volume, refrigerating, stirring, filtering, keeping the temperature of filtrate at 30-80 ℃, adding active carbon with the volume fraction of 0.1-0.5%, stirring for decolorization, filtering, and performing rotary evaporation until the ethanol concentration is lower than 25wt.% to obtain the fructus cnidii leaching solution;

and (3) primary sophora flavescens leaching liquid purification: filtering the sophora flavescens primary leaching solution, adding deionized water with the same volume, refrigerating, stirring, filtering, keeping the temperature of filtrate at 30-80 ℃, adding active carbon with the volume fraction of 0.1-0.5%, stirring for decoloring, filtering, and performing rotary evaporation until the ethanol concentration is lower than 25wt.% to obtain the sophora flavescens leaching solution.

5. The preparation method of the mosquito repellent liquid extracted from pure plants according to claim 4, wherein the refrigeration temperature in the purification steps of the taxus chinensis, the artemisia apiacea, the common cnidium fruit and the radix sophorae flavescentis is 4 ℃, and the refrigeration time is 12-24 hours.

6. The method for preparing the mosquito repellent liquid extracted from the pure plants according to claim 4, wherein the decoloring temperature in the purification steps of the taxus chinensis, the artemisia apiacea, the common cnidium fruit and the radix sophorae flavescentis is 30-80 ℃.

7. The method of preparing a mosquito repellent liquid extracted from pure plants according to claim 6, further comprising the steps of mixing:

mixing the Chinese yew leaching solution, the sweet wormwood leaching solution, the common cnidium fruit leaching solution, the radix sophorae flavescentis leaching solution and deionized water, stirring, sealing, refrigerating at 4 ℃ for 12-24 hours, stirring, standing and filtering to prepare a finished product I.

8. The method for preparing the mosquito repellent liquid extracted from the pure plants according to claim 7, is characterized by further comprising the following steps:

1) preparation of liquid paraffin emulsion

Weighing Arabic gum, adding 80 deg.C distilled water, stirring to dissolve, adding liquid paraffin, emulsifying in tissue triturator, and water bathing at 50 deg.C;

2) preparation of gelatin solution

Weighing type A gelatin, adding distilled water of 60 deg.C, soaking to swell, water bathing at 50 deg.C, stirring to dissolve;

3) preparation of microcapsules

Adding the gelatin solution prepared in the step 2) into the liquid paraffin emulsion prepared in the step 1), stirring, adding the finished product I, adding 10wt.% of acetic acid solution, adjusting the pH value to 3.4-4.1, and stirring to form a microcapsule suspension;

4) curing of capsular membranes

Adding distilled water of 40 ℃ into the microcapsule suspension prepared in the step 3), stirring, cooling, reducing the temperature to 32-36 ℃, adding ice blocks, reducing the temperature to 5 ℃, adding 37% of glutaraldehyde by volume fraction, stirring for 5min, adding 20wt.% of sodium hydroxide to adjust the pH value of the solution to 8.0-8.5, stirring for 30min, solidifying the microcapsules, standing, volatilizing the glutaraldehyde to be odorless, adding 10wt.% of acetic acid solution to adjust the pH value to be neutral, and obtaining a finished product II.

9. The preparation method of the mosquito repellent liquid extracted from the pure plants according to claim 8, wherein the emulsifying time in the step 1) is 5-10 s.

10. The mosquito repellent liquid extracted from pure plants is characterized by being prepared by mixing the following components in parts by volume:

4 parts of a Chinese yew leaching solution;

4 parts of sweet wormwood leaching liquor;

2 parts of sophora flavescens leaching liquor;

2 parts of fructus cnidii leaching liquor;

0.5 part of artemisia argyi leaching liquor;

0.5 part of Pelargonium graveolens leaching solution;

and 37 parts of deionized water.

11. A method of preparing a mosquito repellent liquid extracted from pure plants according to claim 10, comprising the steps of:

1) primary extraction:

adding ethanol into dried yew bark, a overground plant of artemisia apiacea, a root of the sophora flavescens, a mature fruit of fructus cnidii, an overground plant of pelargonium graveolens and an overground plant of artemisia argyi respectively, sealing, placing in a shady, ventilated and dark place, and leaching for 1-7 days to obtain a yew primary leaching solution, an artemisia argyi primary leaching solution, a sophora flavescens primary leaching solution, a fructus cnidii primary leaching solution, a pelargonium graveolens primary leaching solution and an artemisia argyi primary leaching solution;

2) and (3) purification:

filtering the taxus chinensis primary leaching solution, the artemisia apiacea primary leaching solution, the sophora flavescens primary leaching solution, the fructus cnidii primary leaching solution, the pelargonium graveolens primary leaching solution and the artemisia argyi primary leaching solution obtained in the step 1), adding deionized water with the same volume, refrigerating, stirring, filtering, keeping the temperature of the filtrate at 30-80 ℃, adding 0.1-0.5% volume fraction of active carbon, stirring, decoloring, filtering and steaming in a rotating mode until the ethanol concentration is lower than 25wt.%, so as to obtain a taxus chinensis leaching solution, an artemisia apiacea leaching solution, a fructus cnidii leaching solution, an sophora flavescens leaching solution, a pelargonium graveolens leaching solution and an;

3) mixing:

mixing the taxus chinensis leaching solution, the artemisia apiacea leaching solution, the fructus cnidii leaching solution, the radix sophorae flavescentis leaching solution, the geranium leaching solution, the artemisia argyi leaching solution and deionized water obtained in the step 2), stirring, sealing, refrigerating at 4 ℃ for 12-24 hours, stirring, standing and filtering to prepare a finished product I;

4) preparation of microcapsules

a. Preparation of liquid paraffin emulsion

Weighing Arabic gum, adding 80 deg.C distilled water, stirring to dissolve, adding liquid paraffin, emulsifying in tissue triturator, and water bathing at 50 deg.C;

b. preparation of gelatin solution

Weighing type A gelatin, adding distilled water of 60 deg.C, soaking to swell, water bathing at 50 deg.C, stirring to dissolve;

c. preparation of microcapsules

Adding the gelatin solution prepared in the step b into the liquid paraffin emulsion prepared in the step a, stirring, adding a finished product I, adding 10wt.% of acetic acid solution, adjusting the pH value to 3.4-4.1, and stirring to form a microcapsule suspension;

d. curing of capsular membranes

And c, adding distilled water of 40 ℃ into the microcapsule suspension prepared in the step c, stirring, cooling, reducing the temperature to 32-36 ℃, adding ice blocks, reducing the temperature to 5 ℃, adding 37% of glutaraldehyde by volume fraction, stirring for 5min, adding 20wt.% of sodium hydroxide to adjust the pH value of the solution to 8.0-8.5, stirring for 30min, solidifying the microcapsules, standing to volatilize the glutaraldehyde to be odorless, and adding 10wt.% of acetic acid solution to adjust the pH value to be neutral to obtain a finished product II.

Technical Field

The invention belongs to the field of mosquito repellent preparations, and particularly relates to a pure plant-extracted mosquito repellent liquid and a preparation method thereof.

Background

At present, most of the commercially available mosquito repellent liquids are ethanol perfume products prepared by taking floral water oil as a main spice and matching with artificially synthesized Liaoxiang, borneol, deet, anophelifuge ester, menthol, lemon oil, benzoic acid, bergamot acid and Yimening, and the mosquito repellent liquids have certain effects of decontamination, sterilization, itching prevention, mosquito repelling, sweat odor removal and refreshing. However, the mosquito repellent liquid has a not ideal mosquito repellent effect, and a great amount of artificially synthesized Liaoxiang and borneol are added into the product, so that the product has the risk of causing abortion of pregnant women. In addition, the deet and the anophelifuge in the product are effective components of pesticide and have certain toxicity. When a large amount of deet and anophelifuge is inhaled by human body, skin allergy phenomenon appears in a large amount of people, and irreparable damage is caused to human body.

Patent CN 105343919A discloses a preparation method of a yew sachet, which is composed of the following formula: 60-70% of taxus chinensis and 30-40% of active carbon. The invention has the advantages of intelligence development, tranquilization, mosquito and mildew prevention, air purification, fatigue alleviation and the like, but the invention has the following defects: the mosquito repellent effect is not obvious, and the sterilizing and itching relieving effects are not good.

Patent CN 104757038A discloses a micro-emulsified mosquito repellent and a preparation method thereof, which comprises the following formula: 1-5 parts of tetramethrin, 2-6 parts of allethrin, 3-7 parts of piperonyl butoxide, 1-6 parts of salicylic acid, 2-7 parts of ethyl acetate, 3-8 parts of peroxyacetic acid, 2-5 parts of dimethyl phthalate, 1-6 parts of citronellal, 2-7 parts of citronella oil, 3-8 parts of cinnamon oil, 1-6 parts of litsea cubeba oil, 3-6 parts of pine oil, 2-8 parts of pine needle oil, 1-6 parts of citronella oil, 5-12 parts of ethanol, 6-10 parts of an emulsifier, 1-4 parts of an auxiliary emulsifier, 1-5 parts of an antifreezing agent, 2-6 parts of an antifoaming agent and 6-15 parts of water. The micro-emulsified mosquito repellent disclosed by the invention is good in mosquito repellent effect, long in duration and low in toxicity, but the micro-emulsified mosquito repellent disclosed by the invention is relatively complex in components, contains chemical mosquito repellent components such as tetramethrin, allethrin and the like, and is harmful to people who are easy to irritate.

Patent CN 104586694A discloses a floral water for refreshing, repelling mosquito and relieving itching and a preparation method thereof, which comprises the following formula: 0.5-5 mL of star anise extract, 5-15 mL of folium artemisiae argyi extract, 0.1-1.5 g of menthol, 0.01-1 g of borneol, 0.5-4 g of sodium chloride, 0-4000.5-3 mL of PEG, 0.01-0.1 mL of sodium citrate of 0.25 g/mL, 10.01-1 g of vitamin B, 0.01-0.05 mL of essence, 10-50 mL of 75% alcohol and 40-100 mL of distilled water. The toilet water disclosed by the invention is fragrant in smell, can effectively repel mosquitoes, has the effects of cooling and relieving itching, clearing heat and removing toxicity, calming and relieving skin, and eliminating discomfort such as redness and swelling, pruritus and the like, but contains the artificially synthesized borneol, so that the toilet water is harmful to pregnant women and infants, and needs to be used with caution.

Disclosure of Invention

The invention provides a pure plant-extracted mosquito repellent liquid, which has an ideal mosquito repellent effect, does not contain toxic chemical substances, can also reduce swelling and relieve itching, is suitable for pregnant women and people who are easy to irritate, avoids the danger that the conventional mosquito repellent liquid is easy to cause abortion and allergy, solves the technical problems that the conventional mosquito repellent liquid on the market has a poor mosquito repellent effect and is not suitable for infants, pregnant women and people who are easy to irritate, and also provides a preparation method of the mosquito repellent liquid.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows:

the mosquito repellent liquid extracted from pure plants is prepared from the following raw materials in parts by volume:

2-4 parts of a Chinese yew leaching solution;

3-4 parts of a sweet wormwood herb leaching solution;

1-2 parts of sophora flavescens leaching liquor;

1-2 parts of fructus cnidii leaching liquor;

87 parts of deionized water.

Preferably, the composition is prepared by mixing the following components in parts by volume:

4 parts of a Chinese yew leaching solution;

4 parts of sweet wormwood leaching liquor;

2 parts of sophora flavescens leaching liquor;

2 parts of fructus cnidii leaching liquor;

87 parts of deionized water.

The preparation method of the mosquito repellent liquid extracted from pure plants comprises the following steps of primary extraction:

primarily extracting the taxus chinensis: weighing dried taxus chinensis barks, adding ethanol, sealing, and leaching for 1-7 days in a shady, ventilated and dark place to obtain a primary taxus chinensis leaching solution;

primary extraction of sweet wormwood herb: weighing dried over-ground plants of the sweet wormwood herb, adding ethanol, sealing, placing in a shady, ventilated and dark place, and leaching for 1-7 days to obtain a primary sweet wormwood herb leaching solution;

primarily extracting the radix sophorae flavescentis: weighing dried radix sophorae flavescentis, adding ethanol, sealing, placing in a shady, ventilated and dark place, and leaching for 1-7 days to obtain a primary radix sophorae flavescentis leaching solution;

primary extraction of fructus cnidii: weighing dried fructus cnidii mature fruits, adding ethanol, sealing, and leaching in a shady, ventilated and dark place for 1-7 days to obtain a fructus cnidii primary leaching solution.

Preferably, the steps further comprise purifying:

purifying a primary Chinese yew leaching solution: filtering the taxus chinensis primary leaching solution, adding deionized water with the same volume, refrigerating, stirring, filtering, keeping the temperature of filtrate at 30-80 ℃, adding active carbon with the volume fraction of 0.1-0.5%, stirring for decolorization, filtering, and performing rotary evaporation until the ethanol concentration is lower than 25wt.% to obtain a taxus chinensis leaching solution;

and (3) purifying a sweet wormwood primary leaching solution: filtering the sweet wormwood primary leaching solution, adding deionized water with the same volume, refrigerating, stirring, filtering, keeping the temperature of filtrate at 30-80 ℃, adding active carbon with the volume fraction of 0.1-0.5%, stirring, decoloring, filtering, and performing rotary evaporation until the ethanol concentration is lower than 25 wt%, thereby obtaining sweet wormwood leaching solution;

and (3) purifying a fructus cnidii primary leaching solution: filtering the fructus cnidii primary leaching solution, adding deionized water with the same volume, refrigerating, stirring, filtering, keeping the temperature of filtrate at 30-80 ℃, adding active carbon with the volume fraction of 0.1-0.5%, stirring for decolorization, filtering, and performing rotary evaporation until the ethanol concentration is lower than 25wt.% to obtain the fructus cnidii leaching solution;

and (3) primary sophora flavescens leaching liquid purification: filtering the sophora flavescens primary leaching solution, adding deionized water with the same volume, refrigerating, stirring, filtering, keeping the temperature of filtrate at 30-80 ℃, adding active carbon with the volume fraction of 0.1-0.5%, stirring for decoloring, filtering, and performing rotary evaporation until the ethanol concentration is lower than 25wt.% to obtain the sophora flavescens leaching solution.

Preferably, the refrigeration temperature in the purification steps of the taxus chinensis, the sweet wormwood herb, the common cnidium fruit and the lightyellow sophora root is 4 ℃, and the refrigeration time is 12-24 hours.

Preferably, the decoloring temperature in the purification steps of the taxus chinensis, the sweet wormwood herb, the common cnidium fruit and the lightyellow sophora root is 30-80 ℃.

Preferably, the steps further comprise mixing:

mixing the Chinese yew leaching solution, the sweet wormwood leaching solution, the common cnidium fruit leaching solution, the radix sophorae flavescentis leaching solution and deionized water, stirring, sealing, refrigerating at 4 ℃ for 12-24 hours, stirring, standing and filtering to prepare a finished product I.

Preferably, the steps further comprise the preparation of microcapsules:

1) preparation of liquid paraffin emulsion

Weighing Arabic gum, adding 80 deg.C distilled water, stirring to dissolve, adding liquid paraffin, emulsifying in tissue triturator, and water bathing at 50 deg.C;

2) preparation of gelatin solution

Weighing type A gelatin, adding distilled water of 60 deg.C, soaking to swell, water bathing at 50 deg.C, stirring to dissolve;

3) preparation of microcapsules

Adding the gelatin solution prepared in the step 2) into the liquid paraffin emulsion prepared in the step 1), stirring, adding the finished product I, adding 10wt.% of acetic acid solution, adjusting the pH value to 3.4-4.1, and stirring to form a microcapsule suspension;

4) curing of capsular membranes

Adding distilled water of 40 ℃ into the microcapsule suspension prepared in the step 3), stirring, cooling, reducing the temperature to 32-36 ℃, adding ice blocks, reducing the temperature to 5 ℃, adding 37% of glutaraldehyde by volume fraction, stirring for 5min, adding 20wt.% of sodium hydroxide to adjust the pH value of the solution to 8.0-8.5, stirring for 30min, solidifying the microcapsules, standing, volatilizing the glutaraldehyde to be odorless, adding 10wt.% of acetic acid solution to adjust the pH value to be neutral, and obtaining a finished product II.

Preferably, the emulsifying time in the step 1) is 5-10 s.

The mosquito repellent liquid extracted from pure plants is prepared by mixing the following components in parts by volume:

4 parts of a Chinese yew leaching solution;

4 parts of sweet wormwood leaching liquor;

2 parts of sophora flavescens leaching liquor;

2 parts of fructus cnidii leaching liquor;

0.5 part of artemisia argyi leaching liquor;

0.5 part of Pelargonium graveolens leaching solution;

and 37 parts of deionized water.

The preparation method of the mosquito repellent liquid extracted from pure plants comprises the following steps:

1) primary extraction:

adding ethanol into dried yew bark, a overground plant of artemisia apiacea, a root of the sophora flavescens, a mature fruit of fructus cnidii, an overground plant of pelargonium graveolens and an overground plant of artemisia argyi respectively, sealing, placing in a shady, ventilated and dark place, and leaching for 1-7 days to obtain a yew primary leaching solution, an artemisia argyi primary leaching solution, a sophora flavescens primary leaching solution, a fructus cnidii primary leaching solution, a pelargonium graveolens primary leaching solution and an artemisia argyi primary leaching solution;

2) and (3) purification:

filtering the taxus chinensis primary leaching solution, the artemisia apiacea primary leaching solution, the sophora flavescens primary leaching solution, the fructus cnidii primary leaching solution, the pelargonium graveolens primary leaching solution and the artemisia argyi primary leaching solution obtained in the step 1), adding deionized water with the same volume, refrigerating, stirring, filtering, keeping the temperature of the filtrate at 30-80 ℃, adding 0.1-0.5% volume fraction of active carbon, stirring, decoloring, filtering and steaming in a rotating mode until the ethanol concentration is lower than 25wt.%, so as to obtain a taxus chinensis leaching solution, an artemisia apiacea leaching solution, a fructus cnidii leaching solution, an sophora flavescens leaching solution, a pelargonium graveolens leaching solution and an;

3) mixing:

mixing the taxus chinensis leaching solution, the artemisia apiacea leaching solution, the fructus cnidii leaching solution, the radix sophorae flavescentis leaching solution, the geranium leaching solution, the artemisia argyi leaching solution and deionized water obtained in the step 2), stirring, sealing, refrigerating at 4 ℃ for 12-24 hours, stirring, standing and filtering to prepare a finished product I;

4) preparation of microcapsules

a. Preparation of liquid paraffin emulsion

Weighing Arabic gum, adding 80 deg.C distilled water, stirring to dissolve, adding liquid paraffin, emulsifying in tissue triturator, and water bathing at 50 deg.C;

b. preparation of gelatin solution

Weighing type A gelatin, adding distilled water of 60 deg.C, soaking to swell, water bathing at 50 deg.C, stirring to dissolve;

c. preparation of microcapsules

Adding the gelatin solution prepared in the step b into the liquid paraffin emulsion prepared in the step a, stirring, adding a finished product I, adding 10wt.% of acetic acid solution, adjusting the pH value to 3.4-4.1, and stirring to form a microcapsule suspension;

d. curing of capsular membranes

And c, adding distilled water of 40 ℃ into the microcapsule suspension prepared in the step c, stirring, cooling, reducing the temperature to 32-36 ℃, adding ice blocks, reducing the temperature to 5 ℃, adding 37% of glutaraldehyde by volume fraction, stirring for 5min, adding 20wt.% of sodium hydroxide to adjust the pH value of the solution to 8.0-8.5, stirring for 30min, solidifying the microcapsules, standing to volatilize the glutaraldehyde to be odorless, and adding 10wt.% of acetic acid solution to adjust the pH value to be neutral to obtain a finished product II.

Compared with the prior art, the invention has the following beneficial effects:

1) the mosquito repellent liquid is prepared by mixing and processing pure plant extract, does not contain various chemical components such as mosquito repellent ester, borneol and the like, and is suitable for infants, pregnant women and people who are easy to be allergic.

2) The formula is reasonable, and the product has the effects of killing bacteria and relieving itching while repelling mosquitoes:

the taxus chinensis is mild in nature and taste, enters kidney channels, and also has the effects of clearing heat and removing toxicity, expelling parasites, eliminating food retention, eliminating pathogenic factors and dissipating stagnation;

herba Artemisiae Annuae can be used for treating yin deficiency due to pathogenic warming, night fever, yin deficiency fever, hectic fever due to yin deficiency, summer fever due to pathogenic summer heat, malaria fever due to cold, and jaundice due to damp-heat pathogen;

the lightyellow sophora root has good anti-inflammatory effect, is usually used for treating dysentery with fever, hematochezia, jaundice anuria, leucorrhea with red and white discharge, vulvar swelling and pruritus vulvae, eczema, skin pruritus, mange and leprosy, and is externally used for treating trichomonas vaginitis;

fructus Cnidii has effects of clearing heat, eliminating dampness, dispelling pathogenic wind, relieving itching, treating kidney capsule wind, dry and itchy, and preferring bath hot soup, even pimple, shaped like red millet, numb itch, scratching, soaking in lipid water, and skin heat pain like flaming.

3) The mosquito repellent effect is good, and the duration is long.

Detailed Description

Embodiments of the present invention will now be described in detail with reference to the following examples, which should not be construed as limiting the scope of the invention as claimed. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

1) Preliminary extraction

Weighing taxus chinensis branches, removing soil and impurities, cleaning, cutting into small sections, putting the small sections into an oven to be dried for 2-10 hours, taking out, peeling barks, weighing the dried barks, putting the barks into another sealable glass container, adding 75-95 wt.% of ethanol, sealing, placing the sealed container in a shady, ventilated and lightproof place to be leached for 1-7 days, and obtaining a taxus chinensis primary leaching solution;

weighing radix sophorae flavescentis, removing soil and impurities, cleaning, cutting into small sections, drying in an oven for 2-10 hours, taking out, weighing dried radix sophorae flavescentis, putting the dried radix sophorae flavescentis into another sealable glass container, adding 75-95 wt.% of ethanol, sealing, placing in a cool, ventilated and dark place, and leaching for 1-7 days to obtain a primary radix sophorae flavescentis leaching solution;

weighing overground plants of the sweet wormwood, removing soil and impurities, cleaning, cutting into small sections, putting the small sections into an oven to be dried for 2-10 hours, taking out the small sections, weighing the dried sweet wormwood, putting the sweet wormwood into another sealable glass container, adding 75-95 wt.% of ethanol, sealing, and placing the container in a cool, ventilated and light-proof place to be leached for 1-7 days; obtaining a sweet wormwood primary leaching solution;

weighing the ripe fruit of the fructus cnidii, cleaning, putting the fruit into an oven to dry for 2-10 hours, taking out, weighing the dried fructus cnidii, putting the dried fructus cnidii into another sealable glass container, adding 75-95 wt.% of ethanol, sealing, and placing the container in a shady, ventilated and dark place to leach for 1-7 days to obtain a primary leaching solution of the fructus cnidii.

The ethanol is preferably used in an amount just over the medicinal materials.

2) Purification of

Filtering the preliminary leaching liquor of the taxus chinensis obtained in the step 1), adding deionized water with the same volume, and placing the mixture in a refrigerator at 4 ℃ for refrigerating for 12-24 hours. Taking out, stirring lightly, filtering, wherein ten million of filtering can not be performed, the filtering speed can not be too high, replacing the filter paper in time, keeping the obtained primary filtrate at the constant temperature of 30-80 ℃, adding active carbon with the mass of 0.1-0.5% of the primary filtrate, and placing the primary filtrate on a magnetic stirrer to stir at the temperature of 30-80 ℃ for 5-10 min so as to decolor the primary filtrate. Filtering again, placing the obtained fine filtrate in a rotary evaporator for rotary evaporation until the concentration of ethanol is lower than 25wt.%, and obtaining a taxus chinensis leaching liquor;

filtering the primary leaching liquor of the sweet wormwood obtained in the step 1), adding deionized water with the same volume, and placing the mixture in a refrigerator at 4 ℃ for cooling for 12-24 h. Taking out, stirring gently, filtering, wherein ten million of the primary filtrate can not be subjected to suction filtration, the filtering speed can not be too high, replacing the filter paper in time, keeping the temperature of the obtained primary filtrate constant to 30-80 ℃, adding active carbon with the mass equivalent to 0.1-0.5% of that of the primary filtrate, placing the primary filtrate on a magnetic stirrer, and stirring for 5-10 min at the temperature of 30-80 ℃. Filtering again, placing the obtained fine filtrate in a rotary evaporator for rotary evaporation until the ethanol concentration is lower than 25wt.%, and obtaining a sweet wormwood herb leaching liquor;

filtering the primary sophora flavescens leaching solution obtained in the step 1), adding deionized water with the same volume, and placing the mixture in a refrigerator at 4 ℃ for refrigerating for 12-24 hours. Taking out, stirring gently, filtering, wherein ten million of the primary filtrate can not be subjected to suction filtration, the filtering speed can not be too high, replacing the filter paper in time, keeping the temperature of the obtained primary filtrate constant to 30-80 ℃, adding active carbon with the mass equivalent to 0.1-0.5% of that of the primary filtrate, placing the primary filtrate on a magnetic stirrer, and stirring for 5-10 min at the temperature of 30-80 ℃. Filtering again, placing the obtained fine filtrate in a rotary evaporator, and performing rotary evaporation until the ethanol concentration is lower than 25wt.% to obtain radix Sophorae Flavescentis extract;

filtering the fructus cnidii primary leaching solution obtained in the step 1), adding deionized water with the same volume, and placing the fructus cnidii primary leaching solution in a refrigerator at 4 ℃ for cooling for 12-24 hours. Taking out, stirring gently, filtering, wherein ten million of the primary filtrate can not be subjected to suction filtration, the filtering speed can not be too high, replacing the filter paper in time, keeping the temperature of the obtained primary filtrate constant to 30-80 ℃, adding active carbon with the mass equivalent to 0.1-0.5% of that of the primary filtrate, placing the primary filtrate on a magnetic stirrer, and stirring for 5-10 min at the temperature of 30-80 ℃. Filtering again, placing the obtained fine filtrate in a rotary evaporator, and performing rotary evaporation until the ethanol concentration is lower than 25wt.%, to obtain fructus Cnidii leaching liquor;

3) mixing

Mixing the four leaching solutions of the sweet wormwood herb, the radix sophorae flavescentis, the fructus cnidii and the taxus chinensis prepared in the step 2) and deionized water according to a certain volume ratio, stirring uniformly, sealing, then placing in a refrigerator at 4 ℃ for cooling for 12-24 hours, taking out, stirring, standing and filtering to prepare a finished product I.