阅读说明：本技术 利用动物血液制备有机肥的工艺方法 (Process for preparing organic fertilizer by using animal blood ) 是由 王勃森 张芬芳 杨宏伟 史大虎 曹喜全 刘建武 王新江 安宝军 刘军根 吕战峰 于 2019-10-25 设计创作，主要内容包括：提供一种利用动物血液制备有机肥的工艺方法,本发明充分利用了屠宰场原来需要花费大量资金处理的牲畜血液为原料,将其通过多级水解蛋白质、乳化、酶解、发酵等生物处理后,变为微生物有机肥还田,不产生二次处理尾料,不但彻底解决了对环境造成的污染问题,提高附加值,而且其制作的有机肥料以改善土壤有益微生物群落为主,达到有效改良土壤,提高土壤活性,提高作物产量,改善果实品质的效果明。(The invention provides a process method for preparing organic fertilizer by using animal blood, which fully utilizes the livestock blood which originally needs to spend a large amount of capital treatment in a slaughterhouse as a raw material, and the livestock blood is changed into microbial organic fertilizer to be returned to the field after being subjected to biological treatment such as multi-stage protein hydrolysis, emulsification, enzymolysis, fermentation and the like, so that no secondary treatment tailing is generated, thereby not only thoroughly solving the pollution problem caused to the environment and improving the additional value, but also the prepared organic fertilizer is mainly used for improving the beneficial microbial community of soil, and achieving the effects of effectively improving the soil, improving the soil activity, improving the crop yield and improving the fruit quality.)

1. The process method for preparing the organic fertilizer by using the animal blood is characterized by comprising the following steps of: the method comprises the following steps:

1) blood collection: sterilizing the neck of a healthy animal before slaughtering, bleeding the carotid artery, collecting the animal blood into a blood tank, adding 0.55 mass percent of sodium citrate into the fresh animal blood, fully mixing, and uniformly stirring to form an anticoagulant raw material;

2) raw material treatment: putting the anticoagulation raw material into a reaction tank, adding concentrated HCl with the mass fraction of 37.5% into the reaction tank for acid treatment to form a feed liquid with the pH value of 2.5-3.5, and standing for 3 hours;

3) multi-stage hydrolysis of protein:

adding acid protease into the feed liquid in a reaction tank to enable the final concentration of the enzyme to reach 300U/g, and carrying out enzymolysis at the constant temperature of 40-50 ℃ for 6 hours;

secondly, adding caustic soda into the reaction tank according to the proportion of 10mol/L, and adjusting the pH value of the feed liquid to 6.5-8;

thirdly, adding neutral protease and a complex enzyme preparation PTM-012 into the reaction tank to enable the final concentration of the enzyme to reach 800U/g, and carrying out enzymolysis at the constant temperature of 45-50 ℃ for 10 hours;

adding trypsin and flavourzyme into the reaction tank according to the proportion of 0.2-0.8%, and carrying out enzymolysis at the constant temperature of 53-55 ℃ for 3-20 h;

4) emulsification: loading the material liquid subjected to multistage hydrolysis in a reaction tank into a high-shear homogenizing emulsifying machine for homogenizing and emulsifying;

5) enzymolysis: pumping the emulsified feed liquid into a reaction kettle through a centrifugal pump, and carrying out enzymolysis at the constant temperature of 50-55 ℃ for 6-8 h;

6) cooling and fermenting: cooling the feed liquid after the enzymatic degradation to 37 ℃, adding a strain TPJ-01, bacillus subtilis, saccharomyces, lactobacillus and the like according to the proportion of 1:10, fermenting at the constant temperature of 37-38 ℃ for 8 hours;

7) high-temperature sterilization: sterilizing the fermented feed liquid at the high temperature of 115-121 ℃ for 30 min;

8) and (3) concentration detection: repeatedly concentrating the sterilized feed liquid until the concentration of the detected amino acid reaches 10 percent of the national standard, thus forming the organic fertilizer;

9) subpackaging and storing: and packaging and warehousing the organic fertilizer which is detected to reach the national standard, and storing at low temperature.

2. The process method for preparing organic fertilizer by using animal blood as claimed in claim 1, wherein the process method comprises the following steps: in the step 1), the animal blood is pig blood, cattle blood or sheep blood.

Technical Field

The invention belongs to the technical field of fertilizer manufacturing, and particularly relates to a process method for preparing an organic fertilizer by using animal blood.

Background

Along with the increasing living standard of people, the demand of meat products is larger and larger, thereby driving the unprecedented development of the breeding industry. In order to ensure the food safety, the state forces the domestic animals such as live pigs, live cattle and the like to be slaughtered after being completely quarantined and qualified and then enter the market. The domestic livestock slaughtering amount is large every year, a large amount of livestock blood is generated by centralized slaughtering, the blood is generally treated by direct discharge, and the direct discharge can cause environmental pollution. However, the blood of livestock is rich in animal protein and grease, and the animal protein, the grease and other substances are excellent organic fertilizer sources. Therefore, the organic fertilizer is prepared by utilizing the blood of the livestock, which not only can solve the problem of environmental protection and change waste into valuable, but also can provide high-quality organic fertilizer for green agricultural production, reduce the using amount of chemical fertilizer and have higher economic benefit, environmental protection benefit and social benefit. There is therefore a need for improvements.

Disclosure of Invention

The technical problems solved by the invention are as follows: the invention provides a process method for preparing organic fertilizer by using animal blood, which fully utilizes the original livestock blood needing large amount of capital treatment in slaughter houses as raw material, turns the raw material into microbial organic fertilizer to be returned to the field after biological treatment, does not produce secondary treatment tailings, not only thoroughly solves the problem of environmental pollution and improves the added value, but also the prepared fertilizer mainly improves the beneficial microbial community in soil, and achieves the effects of effectively improving the soil, improving the soil activity, improving the crop yield and improving the fruit quality.

The technical scheme adopted by the invention is as follows: the process method for preparing the organic fertilizer by using the animal blood is characterized by comprising the following steps of: the method comprises the following steps:

1) blood collection: sterilizing the neck of a healthy animal before slaughtering, bleeding the carotid artery, collecting the animal blood into a blood tank, adding 0.55 mass percent of sodium citrate into the fresh animal blood, fully mixing, and uniformly stirring to form an anticoagulant raw material;

2) raw material treatment: putting the anticoagulation raw material into a reaction tank, adding concentrated HCl with the mass fraction of 37.5% into the reaction tank for acid treatment to form a feed liquid with the pH value of 2.5-3.5, and standing for 3 hours;

3) multi-stage hydrolysis of protein:

adding acid protease into the feed liquid in a reaction tank to enable the final concentration of the enzyme to reach 300U/g, and carrying out enzymolysis at the constant temperature of 40-50 ℃ for 6 hours;

secondly, adding caustic soda into the reaction tank according to the proportion of 10mol/L, and adjusting the pH value of the feed liquid to 6.5-8;

thirdly, adding neutral protease and a complex enzyme preparation PTM-012 into the reaction tank to enable the final concentration of the enzyme to reach 800U/g, and carrying out enzymolysis at the constant temperature of 45-50 ℃ for 10 hours;

adding trypsin and flavourzyme into the reaction tank according to the proportion of 0.2-0.8%, and carrying out enzymolysis at the constant temperature of 53-55 ℃ for 3-20 h;

4) emulsification: loading the material liquid subjected to multistage hydrolysis in a reaction tank into a high-shear homogenizing emulsifying machine for homogenizing and emulsifying;

5) enzymolysis: pumping the emulsified feed liquid into a reaction kettle through a centrifugal pump, and carrying out enzymolysis at the constant temperature of 50-55 ℃ for 6-8 h;

6) cooling and fermenting: cooling the feed liquid after the enzymatic degradation to 37 ℃, adding a strain TPJ-01, bacillus subtilis, saccharomyces, lactobacillus and the like according to the proportion of 1:10, fermenting at the constant temperature of 37-38 ℃ for 8 hours;

7) high-temperature sterilization: sterilizing the fermented feed liquid at the high temperature of 115-121 ℃ for 30 min;

8) and (3) concentration detection: repeatedly concentrating the sterilized feed liquid until the concentration of the detected amino acid reaches 10 percent of the national standard, thus forming the organic fertilizer;

9) subpackaging and storing: and packaging and warehousing the organic fertilizer which is detected to reach the national standard, and storing at low temperature.

In the step 1), the animal blood is pig blood, cattle blood or sheep blood.

Compared with the prior art, the invention has the advantages that:

1. according to the scheme, different proteins in blood are decomposed by adopting multi-stage protein hydrolyzates, wherein firstly, acidic protease is adopted to effectively hydrolyze the proteins in the blood under the condition of low PH, the cell wall structure among particles of raw materials is damaged, the action of diastase is facilitated, the available carbon source in the raw materials is increased, and the amino acid content is increased; then, under the condition of neutral pH, firstly, utilizing enzymatic reaction of neutral protease to hydrolyze macromolecular protein in animal blood into small molecular peptide or amino acid, then utilizing trypsin to have selective hydrolysis action on arginine and lysine peptide chains, hydrolyzing natural protein, denatured protein, fibrin, mucin and the like into polypeptide or amino acid, and adding proper amount of flavor protease to cut off peptide bonds in the polypeptide to form short-chain peptide so as to improve the hydrolysis degree; after the scheme is subjected to multi-stage proteolysis, the hydrolysis degree of protein substances in blood is greatly improved, and the content of amino acids in materials is improved.

2. The scheme adopts a high-shear emulsifying machine for emulsification, utilizes the powerful kinetic energy brought by high-shear linear velocity and high-frequency mechanical effect to enable the blood feed liquid to be subjected to comprehensive actions of strong mechanical and hydraulic shearing, centrifugal extrusion, liquid layer friction, impact tearing, turbulent flow and the like, so that incompatible oily substances and water-based substances in the feed liquid are well and uniformly mixed, the emulsification is instantly, uniformly and finely dispersed, and stable high-quality products are finally obtained through high-frequency circulation reciprocation.

3. The scheme makes full use of organic matters which need to spend a large amount of capital for processing originally in slaughterhouses, the organic matters are changed into microbial organic fertilizers to be returned to the field after biological treatment, secondary treatment tailings are not generated, environmental pollution is thoroughly solved, the added value is improved, and the prepared fertilizer mainly improves the beneficial microbial community of soil, so that the effects of effectively improving the soil, improving the soil activity, improving the crop yield and improving the fruit quality are achieved.

Drawings

Fig. 1 is a schematic field diagram of a test cell in an embodiment of the invention.

Detailed Description

The following are examples of the present invention.

The process method for preparing the organic fertilizer by using the animal blood comprises the following steps:

1) blood collection: sterilizing the neck of a healthy animal before slaughtering, bleeding the carotid artery, collecting the animal blood into a blood tank, adding 0.55 mass percent of sodium citrate into the fresh animal blood, fully mixing, and uniformly stirring to form an anticoagulant raw material; the animal blood is pig blood, cattle blood or sheep blood.

2) Raw material treatment: putting the anticoagulation raw material into a reaction tank, adding concentrated HCl with the mass fraction of 37.5% into the reaction tank for acid treatment to form a feed liquid with the pH value of 2.5-3.5, and standing for 3 hours;

3) multi-stage hydrolysis of protein:

adding acid protease into the feed liquid in a reaction tank to enable the final concentration of the enzyme to reach 300U/g, and carrying out enzymolysis at the constant temperature of 40-50 ℃ for 6 hours;

secondly, adding caustic soda into the reaction tank according to the proportion of 10mol/L, and adjusting the pH value of the feed liquid to 6.5-8;

thirdly, adding neutral protease and a complex enzyme preparation PTM-012 into the reaction tank to enable the final concentration of the enzyme to reach 800U/g, and carrying out enzymolysis at the constant temperature of 45-50 ℃ for 10 hours;

adding trypsin and flavourzyme into the reaction tank according to the proportion of 0.2-0.8%, and carrying out enzymolysis at the constant temperature of 53-55 ℃ for 3-20 h;

the method comprises the following steps of decomposing different proteins in blood by adopting multi-stage hydrolyzed protein, wherein firstly, acidic protease is adopted, and the acidic protease can effectively hydrolyze the proteins in the blood under the condition of low pH, destroy the cell wall structure among raw material particles, is beneficial to the action of saccharifying enzyme, increases the available carbon source in the raw material and improves the content of amino acid; then, under the condition of neutral pH, the enzymatic reaction of neutral protease is firstly utilized, the neutral protease is obtained by fermenting and extracting bacillus subtilis, belongs to an endonuclease, and can hydrolyze macromolecular protein in animal blood into micromolecular peptide or amino acid at a certain temperature and pH value; then, trypsin is utilized to have selective hydrolysis effect on arginine and lysine peptide chains, so that natural protein, denatured protein, fibrin, mucin and the like can be hydrolyzed into polypeptide or amino acid; the peptide bond in the polypeptide is cut off by adding a proper amount of flavourzyme to form short-chain peptide containing aminopeptidase and carboxypeptidase, and the hydrolysis degree is improved by hydrolyzing the polypeptide at the tail end. The invention greatly improves the hydrolysis degree of protein substances in blood and provides the content of amino acids in materials after multi-stage proteolysis.

4) Emulsification: and (3) loading the material liquid subjected to multistage protein hydrolysis in the reaction tank into a high-shear homogenizing and emulsifying machine for homogenizing and emulsifying. The high-shear emulsifying machine is adopted for emulsification, the blood material liquid is subjected to comprehensive actions of strong mechanical and hydraulic shearing, centrifugal extrusion, liquid layer friction, impact tearing, turbulent flow and the like by utilizing strong kinetic energy brought by high-shear linear velocity and high-frequency mechanical effect, incompatible oily substances and water-based substances in the material liquid are well and uniformly mixed, the oily substances and the water-based substances are instantly, uniformly and finely dispersed and emulsified, and a stable high-quality product is finally obtained through high-frequency circulation reciprocation.

5) Enzymolysis: pumping the emulsified feed liquid into a reaction kettle through a centrifugal pump, and carrying out enzymolysis at the constant temperature of 50-55 ℃ for 6-8 h;

6) cooling and fermenting: cooling the feed liquid after the enzymatic degradation to 37 ℃, adding a strain TPJ-01, bacillus subtilis, saccharomyces, lactobacillus and the like according to the proportion of 1:10, fermenting at the constant temperature of 37-38 ℃ for 8 hours;

7) high-temperature sterilization: sterilizing the fermented feed liquid at the high temperature of 115-121 ℃ for 30 min;

8) and (3) concentration detection: repeatedly concentrating the sterilized feed liquid until the concentration of the detected amino acid reaches 10 percent of the national standard, thus forming the organic fertilizer;

9) subpackaging and storing: and packaging and warehousing the organic fertilizer which is detected to reach the national standard, and storing at low temperature.

In order to verify the yield-increasing and quality-improving effects of the organic fertilizer on production, a fertilizer effect test is arranged on the kiwi fruit.

The first test scheme is as follows: the test was designed to be repeated 3 times for 4 treatments, the test field was rectangular, randomly arranged, and fertilized normally. Namely: treatment 1: the organic fertilizer; and (3) treatment 2: a sterile substrate; and (3) treatment: clear water; and (4) treatment: blank control.

Second, preparation and implementation of the test

1. The selection of the test site is: a kiwi fruit garden is selected as a test field, the orchard is flat in terrain, moderate and upward in soil fertility, and good in irrigation and drainage conditions.

2. Treatment in test:

irrigation: and a plastic hose is adopted for irrigation, so that the series irrigation is avoided.

Physical and chemical properties of the test field soil: the soil is brown soil, soil, soil, black purple soil and loess. The soil texture is medium soil, and the soil fertility is 6 equal lands (according to the national arable land grade evaluation standard). The soil nutrients are detailed in table 1.

Table 1: current state table of soil nutrient in test field

3. Selecting test crops and varieties: the test crop is kiwi fruit, the variety is slow fragrance, the tree age is 12 years, and the fruiting period is positive. The average yield in the last three years is about 39000kg/hm²Planting density of 1650 plants/hm²Namely: the row spacing is 3m, and the plant spacing is 2 m. Wherein: the pollinating trees are 240 plants/hm²(the ratio of male and female trees is 6:1), and the fruit bearing trees are 1410 plants/hm²。

4. The schematic diagram of the experimental field is shown in figure 1, the area of the experimental field is 96m²(i.e., 16 test trees are supplied to the cell, 4 rows are provided, 4 trees are provided in each row, 14 bearing trees and 2 pollination trees are provided in each cell). The test is repeated for 3 times, and the total test area is 1800m²300 trees were tested.

5. Determination of fertilization period and fertilization amount of test crop

The annual fertilization of the kiwi fruits in the test field is divided into base fertilizers, germinated and expanded fertilizers and fruit-strengthening fertilizers according to local fertilization habits. The fertilizer for the test is applied to the kiwi fruits in 6 to 8 months, and is applied for 3 times along with irrigation water, and the dosage of each time is 300kg/hm². The blank control was not fertilized throughout the year.

6. Management measures such as test field irrigation and the like

Irrigation frequency and irrigation quantity: irrigating for 6 times all year round, wherein the water consumption for each irrigation is 600m³/hm²。

Other farming activities and disasters: the pesticide is used for preventing and treating plant diseases and insect pests for 3-4 times all year round. In spring, the patient is usually harmed by late spring coldness, and the prevention work is timely done.

Third, survey record of experiment

1. Kiwi fruit biological trait record

Recording the biological properties of kiwi fruits: the kiwi fruit sprouts and strips in the last 3 th month, buds are formed along with the strips in the last 4 th month, the kiwi fruit blooms from the last 4 th month to the last 5 th month, artificial pollination is started, fruits are set in the last 5 th month, young fruits begin to grow in the last 6 th month, the fruits rapidly expand from the middle 6 th month to the last 7 th month, the sizes of the fruits are basically formed from the last 8 th month to the middle, and the fruits are ripe, harvested and stored in the middle 10 th month after the middle 8 th month to the last 10 th month.

Investigation of biological properties of kiwi fruits: the biological properties of the kiwi fruits in the test area are investigated, and indexes such as the area of kiwi fruit leaves, the thickness of beancurd sheets, the fresh weight of the beancurd sheets and the like are mainly investigated and measured (see tables 2 and 3 for details). Through investigation, 1, the leaves are dark green and thick, the physiological dry leaves are about 1.2 percent, and the individual fruits have the phenomenon of sunburn; 2, the leaves are greener, the leaves are slightly thick, the physiological dry leaves are about 2.8 percent, and the fruits are slightly more in the process of daily burning; 3, the leaves are light green and thin, the physiological dry leaves are about 4.5 percent, and the daily-burned fruits are more; 4, the treated leaves are yellow green, the leaves are thin, the physiological dry leaves are about 8.4 percent, the fruit sun burn phenomenon is serious, and the percentage of the fruit sun burn is more than 1.5 percent.

Table 2: kiwi fruit biological character questionnaire (leaf area)

Table 3: kiwi fruit biological character questionnaire (leaf area, louver thickness and weight)

2. Kiwi fruit yield factor recording and quality detection

Recording yield factors of kiwi fruits: the kiwi fruit yield factor survey mainly surveys factors such as the size of a single kiwi fruit, the fruiting yield of a single plant tree, the fruiting yield of a cell and the like. Table 4 shows the results of the transverse and longitudinal diameter surveys of kiwi fruits, and analysis of table 4 shows that the fruit size is largest for treatment 1, followed by treatment 2 and smallest for treatment 4.

Table 4: kiwi fruit size questionnaire

Table 5 reflects kiwi fruit weight and plot yield, and analysis of table 5 shows that treatment 1 is the heaviest single fruit, followed by treatment 2, and treatment 4 is the smallest single fruit; from the analysis of the average yield of the single plants, the yield of the single plant is highest in the treatment 1, then the treatment 2 is carried out, and the single fruit in the treatment 4 is lowest; from the cell yield analysis, treatment 1 cell yield was highest, followed by treatment 2, and treatment 4 single fruit was lowest.

Table 5: single fruit weight and cell yield questionnaire table for kiwi fruit experimental field

And (3) quality detection record of kiwi fruits: the kiwi fruits in each treatment area are collected, 2kg of the kiwi fruits in each area are collected and are measured by a soil and fertilizer station laboratory, and the measurement results are detailed in table 6. As can be seen from the results of the measurements, the quality indexes of treatment 1 were the best, followed by treatments 2 and 3, with the differences between treatment 2 and treatment 3 being small and treatment 4 being poor.

Table 6: summary table of quality detection results of fruits processed in kiwi fruit field

Experiments show that the organic fertilizer can improve the beneficial microbial community of soil, effectively improve the soil, improve the activity of the soil, and further improve the yield of crops and the quality of fruits.

The above-mentioned embodiments are merely preferred embodiments of the present invention, which are not intended to limit the scope of the present invention, and therefore, all equivalent changes made by the contents of the claims of the present invention should be included in the claims of the present invention.