阅读说明：本技术 包括肌肉基质的装置以及生产方法和用途 (Device comprising a muscle matrix, method for production and use ) 是由 黄励婷 埃里克·斯特克 纳撒尼尔·巴克拉克 辉·许 于 2018-01-29 设计创作，主要内容包括：提供了组织组合物及其制备方法。该组织组合物可用于治疗或再生肌肉组织。该组合物可以被配置为提供与其他肌肉基质相比增加的强度。(Tissue compositions and methods of making the same are provided. The tissue composition can be used for treating or regenerating muscle tissue. The composition may be configured to provide increased strength compared to other muscle matrices.)

1. A method of preparing a tissue composition, the method comprising:

Providing a tissue sample, wherein the tissue sample comprises a muscle portion and a fascia portion, the muscle portion and fascia portion harvested without separating the muscle portion of tissue from the fascia portion of tissue; and

Treating the tissue sample to produce at least one acellular myofascial matrix.

2. The method of claim 1, wherein treating the tissue comprises contacting the tissue sample with a solution comprising trypsin.

3. The method of claim 2, comprising controlling the duration and concentration of exposure of the trypsin solution so as to retain at least some muscle fibers normally present in the tissue sample prior to treatment.

4. The method of claim 1, wherein the tissue sample is harvested from human muscle tissue.

5. The method of claim 1, wherein the tissue sample is harvested from porcine muscle tissue.

6. The method of claim 1, further comprising exposing the tissue sample to a decellularization solution.

7. The method of claim 6, wherein the decellularization solution comprises at least one of a non-ionic octylphenol ethoxylated surfactant, sodium lauryl sulfate, sodium deoxycholate, and polyoxyethylene (20) sorbitan monolaurate.

8. A tissue composition, the tissue composition comprising:

At least one decellularized muscle matrix;

At least one acellular fascia matrix; and is

Wherein the muscle matrix and the fascia matrix comprise at least one muscle tissue and at least one attached fascia tissue, which are harvested without separating the muscle tissue from the fascia tissue, and wherein the muscle matrix comprises at least some muscle fibers that are normally present in an untreated muscle sample.

9. The tissue composition of claim 8, wherein the at least one muscle sample and the at least one fascia sample comprise human tissue.

10. The tissue composition of claim 8, wherein the at least one muscle sample and the at least one fascia sample comprise porcine muscle tissue.

11. The tissue composition of claim 8, wherein the at least one muscle matrix and the at least one fascia matrix have been decellularized by exposing the at least one muscle sample and the at least one fascia sample to a decellularizing solution.

12. The tissue composition of claim 11, wherein the decellularization solution comprises at least one of a non-ionic octylphenol ethoxylated surfactant, sodium lauryl sulfate, sodium deoxycholate, and polyoxyethylene (20) sorbitan monolaurate.

13. A method of preparing a tissue composition, the method comprising:

Providing a muscle sample; and

Treating the muscle sample to produce a decellularized muscle matrix, wherein muscle fibers of the muscle matrix are oriented in a longitudinal direction.

14. The method of claim 13, wherein treating the tissue comprises contacting the muscle sample with a solution comprising trypsin.

15. The method of claim 14, further comprising controlling the duration and concentration of exposure of the trypsin solution so as to retain at least some muscle fibers normally present in the muscle sample prior to treatment.

16. the method of claim 13, wherein the muscle sample comprises human muscle tissue.

17. The method of claim 13, wherein the muscle sample comprises porcine muscle tissue.

18. The method of claim 13, wherein processing the muscle sample comprises exposing the muscle sample to a decellularization solution.

19. The method of claim 18, wherein the decellularization solution comprises at least one of a non-ionic octylphenol ethoxylated surfactant, sodium lauryl sulfate, sodium deoxycholate, and polyoxyethylene (20) sorbitan monolaurate.

20. A tissue composition, the tissue composition comprising:

At least one decellularized muscle matrix comprising at least some muscle fibers normally present in an untreated muscle sample, wherein the muscle fibers are oriented longitudinally.

21. The tissue composition of claim 20, wherein the at least one muscle matrix is derived from at least one muscle sample harvested from human muscle tissue.

22. The tissue composition of claim 20, wherein the at least one muscle matrix is derived from at least one muscle sample harvested from porcine muscle tissue.

23. The tissue composition of claim 20, wherein the at least one muscle matrix is decellularized by exposing at least one muscle sample to a decellularizing solution.

24. The tissue composition of claim 23, wherein the decellularization solution comprises at least one of a non-ionic octylphenol ethoxylated surfactant, sodium lauryl sulfate, sodium deoxycholate, and polyoxyethylene (20) sorbitan monolaurate.

25. A method of preparing a tissue composition, the method comprising:

Providing a set of muscle matrices; and

The muscle matrices of the groups are stacked and joined to produce a multi-layered muscle matrix.

26. The method of claim 25, wherein the muscle matrix is attached by a dehydrothermal process.

27. The method of claim 25, wherein the muscle matrix is attached by cross-linking.

28. The method of claim 25, wherein the layers of muscle matrix are joined by compression.

29. The method of claim 25, wherein the layers of muscle matrix are connected with a tissue matrix slurry.

30. The method of claim 29, wherein the biological slurry comprises a muscle tissue matrix.

31. The method of claim 25, wherein the layers of muscle matrix are joined by fastening a sheet of muscle tissue matrix.

32. The method of claim 25, wherein the muscle matrix of the set is harvested from human muscle tissue.

33. The method of claim 25, wherein the muscle matrix of the group is harvested from porcine muscle tissue.

34. A tissue composition, the tissue composition comprising:

A multi-layered decellularized muscle matrix comprising at least some muscle fibers normally present in an untreated muscle sample.

35. The composition of claim 34, wherein the muscle matrix is attached by a dehydrothermal process.

36. The composition of claim 34, wherein the muscle matrix is linked by cross-linking.

37. The composition of claim 34, wherein the layers of muscle matrix are connected by compression.

38. The composition of claim 34, wherein the layers of muscle matrix are connected with a tissue matrix slurry.

39. The composition of claim 38, wherein the tissue matrix slurry comprises muscle tissue matrix.

40. The composition of claim 34, wherein the layers of muscle matrix are joined by fastening a sheet of muscle tissue matrix.

41. The composition of claim 34, wherein the muscle matrix of the group is harvested from human muscle tissue.

42. The composition of claim 34, wherein the muscle matrix of the group is harvested from porcine muscle tissue.

43. A method of preparing a tissue composition, the method comprising:

Selecting a muscle matrix layer;

Selecting a support layer;

Applying a slurry comprising particulate Acellular Tissue Matrix (ATM) onto at least one of the muscle matrix layer or the support layer; and

Connecting the muscle matrix layer and the support layer using the slurry.

44. The method of claim 43, wherein the support layer comprises a synthetic substrate.

45. The method of claim 44, wherein the synthetic substrate is a mesh.

46. The method of claim 44, wherein the synthetic substrate is a polypropylene mesh.

47. The method of claim 43, wherein the slurry comprises a dermal tissue matrix or a muscle tissue matrix.

48. The method of claim 43, further comprising freeze drying the slurry to form a porous structure.

49. The method of claim 43, further comprising stabilizing the slurry.

50. The method of claim 43, further comprising:

Applying an additional slurry comprising particulate Acellular Tissue Matrix (ATM) onto the support layer opposite the muscle matrix layer; and

Connecting additional muscle matrix layers to the support layer using the additional slurry.

51. A tissue composition, the tissue composition comprising:

at least one muscle matrix layer;

At least one support layer; and

At least one particulate Acellular Tissue Matrix (ATM) adhering the at least one muscle layer to the at least one support layer.

52. the tissue composition of claim 51, wherein the at least one support layer comprises a synthetic substrate.

53. The tissue composition of claim 52, wherein the synthetic substrate is a mesh.

54. The tissue composition of claim 53, wherein the synthetic substrate is a polypropylene mesh.

55. The tissue composition of claim 51, wherein the at least one particulate ATM comprises a dermal tissue matrix or a muscular tissue matrix.

56. The tissue composition of claim 51, wherein the at least one particulate ATM comprises rehydrated particulates.

57. the tissue composition of claim 51, wherein the at least one particulate ATM comprises a porous structure.

58. The tissue composition of claim 51, wherein the at least one particulate ATM layer is crosslinked.

59. the tissue composition of claim 51, further comprising:

At least one additional granular ATM disposed adjacent to the at least one support layer opposite the at least one muscle matrix layer; and

At least one additional layer of muscle matrix disposed adjacent to the at least one additional dry granular ATM opposite the at least one support layer.

60. A method of preparing a tissue composition, the method comprising:

Selecting a muscle matrix layer;

Selecting a support layer comprising one or more apertures;

applying a slurry comprising particulate Acellular Tissue Matrix (ATM) onto at least one of the muscle matrix layer or the support layer;

applying the support layer to a first surface of the muscle matrix layer; and

Rolling the muscle matrix layer and the support layer such that at least a portion of the first surface of the muscle matrix layer is adhered to at least a portion of a second surface of the muscle matrix layer through the one or more apertures.

61. The method of claim 60, wherein the support layer comprises a synthetic substrate.

62. The method of claim 61, wherein the synthetic substrate is a mesh.

63. The method of claim 62, wherein the synthetic substrate is a polypropylene mesh.

64. The method of claim 60, wherein the support layer comprises a metal mesh.