阅读说明：本技术 一种长效抗菌的聚丙烯无纺布的制备方法 (Preparation method of long-acting antibacterial polypropylene non-woven fabric ) 是由 李祥 于 2021-06-21 设计创作，主要内容包括：本发明公开了一种长效抗菌的聚丙烯无纺布的制备方法,包括将聚丙烯无纺布材料浸泡在丙酮溶液中超声6～10min后捞出,在浸泡入乙醇中超声10～20min,捞出后在45～48℃下真空干燥4～8h；将红菲罗啉溶解到乙醇中配置成1～1.5％的乙醇溶液,将晾干后的聚丙烯无纺布浸入到该溶液中30～50min,取出后晾干；将螺内酯、二苯甲酮溶解在无水乙醇溶液中,将步骤S1中得到的无纺布平铺放置在石英玻璃板上,滴加该溶液,之后在其表面上覆盖石英玻璃板,放置在高压汞灯下反应10～15min,取出该聚丙烯无纺布在丙酮中超声洗涤15～30min,取出在室温下真空干燥；将万古霉素加入到磷酸缓冲液中,超声溶解,将步骤S2中干燥好聚丙烯无纺布浸入到该溶液中,在35～38℃下摇荡反应20～30h,反应结束后用蒸馏水清洗3～5次晾干后得到所述聚丙烯无纺布。(The invention discloses a preparation method of a long-acting antibacterial polypropylene non-woven fabric, which comprises the steps of soaking a polypropylene non-woven fabric material in an acetone solution for 6-10 min by ultrasound, then fishing out, soaking in ethanol for 10-20 min by ultrasound, and then drying in vacuum at 45-48 ℃ for 4-8 h after fishing out; dissolving bathophenanthroline in ethanol to prepare 1-1.5% ethanol solution, soaking the dried polypropylene non-woven fabric in the solution for 30-50 min, taking out and drying; dissolving spironolactone and benzophenone in an absolute ethanol solution, flatly laying the non-woven fabric obtained in the step S1 on a quartz glass plate, dropwise adding the solution, covering the quartz glass plate on the surface of the non-woven fabric, placing the non-woven fabric under a high-pressure mercury lamp for reaction for 10-15 min, taking out the polypropylene non-woven fabric, ultrasonically washing the polypropylene non-woven fabric in acetone for 15-30 min, and taking out the polypropylene non-woven fabric for vacuum drying at room temperature; and adding vancomycin into a phosphate buffer solution, ultrasonically dissolving, soaking the polypropylene non-woven fabric dried in the step S2 into the solution, shaking to react for 20-30 h at 35-38 ℃, washing with distilled water for 3-5 times after the reaction is finished, and airing to obtain the polypropylene non-woven fabric.)

1. A preparation method of a long-acting antibacterial polypropylene non-woven fabric is characterized by comprising the following steps:

s1: soaking the polypropylene non-woven fabric material in an acetone solution, performing ultrasonic treatment for 6-10 min, taking out, drying in the air, soaking in absolute ethyl alcohol, performing ultrasonic treatment for 10-20 min, taking out, and performing vacuum drying at 45-48 ℃ for 4-8 h; dissolving bathophenanthroline in ethanol to prepare 1-1.5% ethanol solution, soaking the dried polypropylene non-woven fabric in the solution for 30-50 min, taking out and drying;

s2: dissolving spironolactone and benzophenone in an absolute ethanol solution, then flatly laying the non-woven fabric obtained in the step S1 on a quartz glass plate, dropwise adding the solution, then covering the quartz glass plate on the surface of the non-woven fabric, placing the non-woven fabric under a high-pressure mercury lamp for reaction for 10-15 min, taking out the polypropylene non-woven fabric, ultrasonically washing the polypropylene non-woven fabric in acetone for 15-30 min, taking out the polypropylene non-woven fabric, and performing vacuum drying at room temperature;

s3: and adding vancomycin into a phosphate buffer solution, ultrasonically dissolving, then soaking the polypropylene non-woven fabric dried in the step S2 into the solution, shaking to react for 20-30 h at 35-38 ℃, and after the reaction is finished, washing with distilled water for 3-5 times and drying to obtain the polypropylene non-woven fabric.

2. The method for preparing a long-lasting antibacterial polypropylene non-woven fabric according to claim 1, wherein the amount of the spironolactone and the benzophenone ethanol solution added in step S2 is 15-22% and 8-18% of the weight of the non-woven fabric.

3. The preparation method of the long-acting antibacterial polypropylene non-woven fabric according to claim 1, wherein the radiation spectrum of the high-pressure mercury lamp is 370-380 nm, and the power is 400-420W.

4. The method for preparing a long-acting antibacterial polypropylene non-woven fabric according to claim 1, wherein the pH value of the phosphate buffer solution in the step S3 is between 5.8 and 7.5.

5. The preparation method of the long-acting antibacterial polypropylene non-woven fabric according to claim 1, wherein the mass ratio of the vancomycin to the phosphate buffer is (5.5-8.2) to (12-20).

Technical Field

The invention belongs to the technical field of non-woven fabric preparation, and particularly relates to a preparation method of a long-acting antibacterial polypropylene non-woven fabric.

Background

Nonwoven fabrics, also known as nonwovens, are composed of oriented or random fibers. It is called a cloth because of its appearance and certain properties. The non-woven fabric has the characteristics of moisture resistance, air permeability, flexibility, light weight, no combustion supporting, easy decomposition, no toxicity or irritation, rich color, low price, recycling and the like. For example, the polypropylene fiber is produced by adopting polypropylene granules as raw materials through a continuous one-step method of high-temperature melting, spinning, laying a line and hot-pressing coiling. The non-woven fabric has no warp and weft, is very convenient to cut and sew, is light in weight and easy to shape, and is popular with hand fans. Because it is a fabric formed without spinning a woven fabric, it is formed by orienting or randomly arranging textile staple fibers or filaments to form a web structure and then reinforcing them by mechanical, thermal or chemical means. It is not woven and knitted by yarns one by one, but the fibers are directly bonded together by a physical method. The non-woven fabric breaks through the traditional spinning principle and has the characteristics of short process flow, high production rate, high yield, low cost, wide application, multiple raw material sources and the like. The polypropylene non-woven fabric has stable chemical performance, good mechanical strength and thermal stability, and can be widely applied to the pharmaceutical industry, water treatment technology, packaging materials and the like. Wherein the polypropylene non-woven fabric is light and soft, has good air permeability and is widely used in the field of medical biomaterials. However, due to the hydrophobic property of polypropylene, bacteria are easy to adhere and breed on the surface of the polypropylene, and then a biological film is formed. The formation of the biological membrane is closely related to persistent bacterial infection, and the bacteria are easy to enhance the tolerance to antibacterial drugs, thereby greatly increasing the difficulty of curing the infection. This severely limits the application of polypropylene non-woven fabrics in the fields of wound dressing, scaffolds for human tissue engineering and the like.

Disclosure of Invention

The invention aims to provide a preparation method of a long-acting antibacterial polypropylene non-woven fabric, which comprises the following steps:

s1: soaking the polypropylene non-woven fabric material in an acetone solution, performing ultrasonic treatment for 6-10 min, taking out, drying in the air, soaking in absolute ethyl alcohol, performing ultrasonic treatment for 10-20 min, taking out, and performing vacuum drying at 45-48 ℃ for 4-8 h; dissolving bathophenanthroline in ethanol to prepare 1-1.5% ethanol solution, soaking the dried polypropylene non-woven fabric in the solution for 30-50 min, taking out and drying.

S2: dissolving spironolactone and benzophenone in an absolute ethyl alcohol solution, then flatly laying the non-woven fabric obtained in the step S1 on a quartz glass plate, dropwise adding the solution, then covering the quartz glass plate on the surface of the non-woven fabric, placing the non-woven fabric under a high-pressure mercury lamp for reaction for 10-15 min, taking out the polypropylene non-woven fabric, ultrasonically washing the polypropylene non-woven fabric in acetone for 15-30 min, taking out the polypropylene non-woven fabric, and performing vacuum drying at room temperature.

S3: and adding vancomycin into a phosphate buffer solution, ultrasonically dissolving, then soaking the polypropylene non-woven fabric dried in the step S2 into the solution, shaking to react for 20-30 h at 35-38 ℃, and after the reaction is finished, washing with distilled water for 3-5 times and drying to obtain the polypropylene non-woven fabric.

Further, the dropping amount of the spironolactone and benzophenone ethanol solution in the step S2 is 15-22% and 8-18% of the weight of the non-woven fabric respectively.

Furthermore, the radiation spectrum of the high-pressure mercury lamp is 370-380 nm, and the power is 400-420W.

Further, the pH value of the phosphate buffer solution in the step S3 is between 5.8 and 7.5.

Furthermore, the mass ratio of the vancomycin to the phosphate buffer is (5.5-8.2): 12-20.

Compared with the prior art, the invention has the following beneficial effects: in the invention, the non-woven fabric material obtained by modifying the polypropylene non-woven fabric by adopting the polypropylene non-woven fabric has excellent and long-term antibacterial effect.

Detailed Description

The following embodiments of the present invention are described in detail, and the embodiments are implemented on the premise of the technical solution of the present invention, and a detailed implementation manner and a specific operation process are given, it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Example 1

A preparation method of a long-acting antibacterial polypropylene non-woven fabric specifically comprises the following steps:

s1: soaking polypropylene non-woven fabric material in acetone solution, performing ultrasonic treatment for 6min, taking out, air drying, soaking in anhydrous ethanol, performing ultrasonic treatment for 10min, taking out, and vacuum drying at 45 deg.C for 4 h; dissolving bathophenanthroline in ethanol to obtain 1% ethanol solution, soaking the dried polypropylene nonwoven fabric in the solution for 30min, taking out, and air drying.

S2: dissolving spironolactone and benzophenone in an absolute ethanol solution, then flatly laying the non-woven fabric obtained in the step S1 on a quartz glass plate, dropwise adding the solution, then covering the quartz glass plate on the surface of the non-woven fabric, placing the non-woven fabric under a high-pressure mercury lamp for 10min for reaction, taking out the polypropylene non-woven fabric, ultrasonically washing the polypropylene non-woven fabric in acetone for 15min, taking out the polypropylene non-woven fabric, and carrying out vacuum drying at room temperature; wherein the dropping amount of the spironolactone and benzophenone ethanol solution is 15 percent and 8 percent of the weight of the non-woven fabric, the radiation spectrum of the high-pressure mercury lamp is 370nm, and the power is 400W.

S3: adding vancomycin into a phosphate buffer solution, dissolving by ultrasonic, then soaking the polypropylene non-woven fabric dried in the step S2 into the solution, shaking for reaction at 35 ℃ for 20 hours, washing with distilled water for 3 times after the reaction is finished, and airing to obtain the polypropylene non-woven fabric; wherein the pH value of the phosphate buffer solution is between 5.8; the mass ratio of the vancomycin to the phosphate buffer solution is 5.5: 12.

Example 2

A preparation method of a long-acting antibacterial polypropylene non-woven fabric specifically comprises the following steps:

s1: soaking the polypropylene non-woven fabric material in acetone solution, performing ultrasonic treatment for 10min, taking out, air drying, soaking in absolute ethanol, performing ultrasonic treatment for 20min, taking out, and vacuum drying at 48 deg.C for 8 h; dissolving bathophenanthroline in ethanol to obtain 1.5% ethanol solution, soaking the dried polypropylene nonwoven fabric in the ethanol solution for 50min, taking out, and air drying.

S2: dissolving spironolactone and benzophenone in an absolute ethanol solution, then flatly laying the non-woven fabric obtained in the step S1 on a quartz glass plate, dropwise adding the solution, then covering the quartz glass plate on the surface of the non-woven fabric, placing the non-woven fabric under a high-pressure mercury lamp for 15min for reaction, taking out the polypropylene non-woven fabric, ultrasonically washing the polypropylene non-woven fabric in acetone for 30min, taking out the polypropylene non-woven fabric, and carrying out vacuum drying at room temperature; wherein the dropping amount of the spironolactone and benzophenone ethanol solution is 22 percent and 18 percent of the weight of the non-woven fabric, the radiation spectrum of the high-pressure mercury lamp is 380nm, and the power is 420W.

S3: adding vancomycin into a phosphate buffer solution, dissolving by ultrasonic, then soaking the polypropylene non-woven fabric dried in the step S2 into the solution, shaking for reaction at 38 ℃ for 30 hours, washing with distilled water for 5 times after the reaction is finished, and airing to obtain the polypropylene non-woven fabric; wherein the pH value of the phosphate buffer solution is 7.5; the mass ratio of the vancomycin to the phosphate buffer solution is 8.2: 20.

Example 3

A preparation method of a long-acting antibacterial polypropylene non-woven fabric specifically comprises the following steps:

s1: soaking the polypropylene non-woven fabric material in acetone solution, performing ultrasonic treatment for 8min, taking out, air drying, soaking in absolute ethanol, performing ultrasonic treatment for 15min, taking out, and vacuum drying at 46 deg.C for 6 h; dissolving bathophenanthroline in ethanol to obtain 1.2% ethanol solution, soaking the dried polypropylene nonwoven fabric in the ethanol solution for 40min, taking out, and air drying.

S2: dissolving spironolactone and benzophenone in an absolute ethanol solution, then flatly laying the non-woven fabric obtained in the step S1 on a quartz glass plate, dropwise adding the solution, then covering the quartz glass plate on the surface of the non-woven fabric, placing the non-woven fabric under a high-pressure mercury lamp for 12min for reaction, taking out the polypropylene non-woven fabric, ultrasonically washing the polypropylene non-woven fabric in acetone for 20min, taking out the polypropylene non-woven fabric, and carrying out vacuum drying at room temperature; wherein the dropping amount of the spironolactone and benzophenone ethanol solution is 18 percent and 12 percent of the weight of the non-woven fabric, the radiation spectrum of the high-pressure mercury lamp is 375nm, and the power is 410W.

S3: adding vancomycin into a phosphate buffer solution, dissolving by ultrasonic, then soaking the polypropylene non-woven fabric dried in the step S2 into the solution, shaking for reaction at 36 ℃ for 25 hours, washing with distilled water for 4 times after the reaction is finished, and airing to obtain the polypropylene non-woven fabric; wherein the pH value of the phosphate buffer solution is 6.3; the mass ratio of the vancomycin to the phosphate buffer solution is 6.6: 15.

Example 4

A preparation method of a long-acting antibacterial polypropylene non-woven fabric specifically comprises the following steps:

s1: soaking the polypropylene non-woven fabric material in acetone solution, performing ultrasonic treatment for 9min, taking out, air drying, soaking in absolute ethanol, performing ultrasonic treatment for 18min, taking out, and vacuum drying at 47 deg.C for 7 h; dissolving bathophenanthroline in ethanol to obtain 1.4% ethanol solution, soaking the dried polypropylene nonwoven fabric in the ethanol solution for 45min, taking out, and air drying.

S2: dissolving spironolactone and benzophenone in an absolute ethanol solution, then flatly laying the non-woven fabric obtained in the step S1 on a quartz glass plate, dropwise adding the solution, then covering the quartz glass plate on the surface of the non-woven fabric, placing the non-woven fabric under a high-pressure mercury lamp for 10-15 min for reaction, taking out the polypropylene non-woven fabric, ultrasonically washing the polypropylene non-woven fabric in acetone for 25min, taking out the polypropylene non-woven fabric, and performing vacuum drying at room temperature; wherein the dropping amount of the spironolactone and benzophenone ethanol solution is 20 percent and 15 percent of the weight of the non-woven fabric, the radiation spectrum of the high-pressure mercury lamp is 378nm, and the power is 415W.

S3: adding vancomycin into a phosphate buffer solution, dissolving by ultrasonic, then soaking the polypropylene non-woven fabric dried in the step S2 into the solution, shaking for reaction at 37 ℃ for 25 hours, washing with distilled water for 5 times after the reaction is finished, and airing to obtain the polypropylene non-woven fabric; wherein the pH value of the phosphate buffer solution is 7.2; the mass ratio of the vancomycin to the phosphate buffer solution is 7.5: 18.

Performance testing：

The results of the tests on the antibacterial rate of the newly prepared nonwoven fabrics of examples 1 to 4 and the nonwoven fabrics of examples 1 to 4 after being washed with 50 times of standard water according to the evaluation of the antibacterial performance of the textile of the standard GB-T/20944.3-2008 are shown in Table 1,

table 1. test results:

as can be seen from table 1, after being washed with standard water for 50 times, the polypropylene nonwoven fabric material with antibacterial performance prepared in embodiments 1 to 4 of the present invention still has an antibacterial rate of 99% or more against staphylococcus aureus, escherichia coli, and candida, and has excellent and durable antibacterial performance.