阅读说明：本技术 一种血液分析仪 (Blood analyzer ) 是由 章颖 邵汉荣 于 2019-10-28 设计创作，主要内容包括：本发明公开了一种血液分析仪,本申请可实现血常规+多种特定蛋白的联合检测,且由于血液分析仪具有多个相互独立但结构相同的特定蛋白检测模块,所以本申请在测量血液样本中多种特定蛋白的含量时可通过不同特定蛋白检测模块实现并行测量,与现有技术中先后测量血液样本中多种特定蛋白的含量相比,本申请的并行测量方式缩短了检测时间,从而可满足于急症检测需求。(The invention discloses a blood analyzer, which can realize the joint detection of blood routine and multiple specific proteins, and because the blood analyzer is provided with multiple specific protein detection modules which are independent from each other and have the same structure, the parallel measurement can be realized by different specific protein detection modules when the content of the multiple specific proteins in a blood sample is measured.)

1. a blood analyzer, comprising:

the mode selection module is used for selecting one or more target measurement modes from the measurement mode set to carry out measurement; wherein the set of measurement modes comprises a blood routine measurement mode and M specific protein measurement modes;

The measurement module comprises a blood routine detection module and N specific protein detection modules which work independently; n, M are integers more than 1, and M is less than or equal to N;

the control module is used for calling the blood routine detection module to carry out blood routine detection on the current blood sample to be detected when the blood routine measurement mode is selected from the mode selection module; and when M specific protein measurement modes are selected from the mode selection module, the M specific protein detection modules are respectively called to carry out one-to-one measurement on the contents of M specific proteins in the blood sample to be detected so as to realize the parallel measurement of the M specific proteins.

2. The blood analyzer of claim 1, wherein when the number of blood samples to be tested is greater than 1, the control module is further configured to:

When the measurement of M specific proteins in the current blood sample to be detected is not finished, calling the specific protein detection module which is not in the detection state to measure the content of the specific proteins in the next blood sample to be detected one by one until all the blood samples to be detected finish the measurement of M specific proteins.

3. The blood analyzer of claim 2, wherein the specific protein detection modules called for the measurement of the same specific protein of different blood samples to be tested are the same specific protein detection module.

4. The hematology analyzer of claim 1, wherein the set of measurement modes further includes a single specific protein measurement mode;

Correspondingly, the control module is further configured to:

When a single specific protein measurement mode is selected from the mode selection module, calling a specific protein detection module to measure the content of the corresponding single specific protein in the current blood sample to be detected; wherein the single specific protein measurement mode is not selected simultaneously with the M specific protein measurement modes.

5. The blood analyzer of claim 4, wherein when the number of blood samples to be tested is greater than 1, the control module is further configured to:

When the single specific protein measurement of the current blood sample to be detected is not completed, the specific protein detection module which is not in the detection state is called to measure the content of the corresponding single specific protein in the next blood sample to be detected until all the blood samples to be detected complete the single specific protein measurement.

6. The hematology analyzer of any one of claims 1-5, wherein the specific proteins include CRP and HbA1 c.

7. The blood analyzer of any of claims 1-5, wherein the blood routine measurement mode comprises a CBC blood cell three class measurement mode and a CBC blood cell five class measurement mode; the blood routine detection module comprises a WBC detection module, an RBC detection module, an HGB detection module and a DIFF detection module;

correspondingly, the control module is specifically configured to:

when a CBC blood cell three-classification measurement mode is selected from the mode selection module, calling the WBC detection module to carry out three-classification and counting on white blood cells in the current blood sample to be detected; calling the RBC detection module to detect red blood cells and platelets in the blood sample to be detected; calling the HGB detection module to detect the concentration of hemoglobin in the current blood sample to be detected;

When the CBC blood cell five-classification measurement mode is selected from the mode selection module, calling the WBC detection module to classify and count basophils in the current blood sample to be detected; calling the RBC detection module to detect red blood cells and platelets in the blood sample to be detected; calling the HGB detection module to detect the concentration of hemoglobin in the current blood sample to be detected; and calling the DIFF detection module to perform four classification and counting on the white blood cells in the blood sample to be detected currently.

8. The hematology analyzer of claim 7, wherein when the specific protein comprises CRP, the control module is specifically configured to:

calling a specific protein detection module to measure the content of CRP in the blood sample to be detected so as to obtain a CRP content value;

And correcting the CRP content value according to the HCT value detected by the RBC detection module, and taking the corrected CRP content value as the CRP content value in the current blood sample to be detected.

9. The blood analyzer of any one of claims 1-5, wherein each of the specific protein detection modules comprises a reaction cell, a colorimetric cell, and a light-receiving module;

Correspondingly, when a specific protein detection module is called to measure the content of a target specific protein in the blood sample to be detected, the control module is specifically configured to:

Adding the current blood sample to be detected and the reaction reagent corresponding to the target specific protein into the reaction tank for reaction, and adding the reacted mixture into the colorimetric tank;

and projecting the first optical signal to the colorimetric pool by using an optical emission module, and obtaining a second optical signal representing the turbidity of the colorimetric pool by using the optical receiving module so as to measure the content of the target specific protein by using an immunoturbidimetry.

10. the blood analyzer of claim 9, wherein a plurality of the specific protein detection modules share the same light emission module.

Technical Field

the invention relates to the field of blood analysis, in particular to a blood analyzer.

Background

GDM (hereditary diabetes) refers to a condition in which pregnant women experience varying degrees of impaired glucose tolerance during pregnancy. At present, many studies show that the occurrence of GDM has a correlation with the contents of CRP (C-reactive protein) and glycated hemoglobin (HbA1C) in human blood, so that the simultaneous measurement of the contents of CRP and HbA1C in human blood is of great significance for the research in the field of pathological pregnancy.

Disclosure of Invention

the invention aims to provide a blood analyzer which can realize parallel measurement through different specific protein detection modules when measuring the content of various specific proteins in a blood sample, thereby shortening the detection time and meeting the requirement of emergency detection.

in order to solve the above technical problem, the present invention provides a blood analyzer, comprising:

The mode selection module is used for selecting one or more target measurement modes from the measurement mode set to carry out measurement; wherein the set of measurement modes comprises a blood routine measurement mode and M specific protein measurement modes;

The measurement module comprises a blood routine detection module and N specific protein detection modules which work independently; n, M are integers more than 1, and M is less than or equal to N;

The control module is used for calling the blood routine detection module to carry out blood routine detection on the current blood sample to be detected when the blood routine measurement mode is selected from the mode selection module; and when M specific protein measurement modes are selected from the mode selection module, the M specific protein detection modules are respectively called to carry out one-to-one measurement on the contents of M specific proteins in the blood sample to be detected so as to realize the parallel measurement of the M specific proteins.

Preferably, when the number of blood samples to be tested is greater than 1, the control module is further configured to:

When the measurement of M specific proteins in the current blood sample to be detected is not finished, calling the specific protein detection module which is not in the detection state to measure the content of the specific proteins in the next blood sample to be detected one by one until all the blood samples to be detected finish the measurement of M specific proteins.

Preferably, the specific protein detection modules called for the measurement of the same specific protein of different blood samples to be detected are the same specific protein detection module.

preferably, the set of measurement patterns further comprises a single specific protein measurement pattern;

correspondingly, the control module is further configured to:

When a single specific protein measurement mode is selected from the mode selection module, calling a specific protein detection module to measure the content of the corresponding single specific protein in the current blood sample to be detected; wherein the single specific protein measurement mode is not selected simultaneously with the M specific protein measurement modes.

Preferably, when the number of blood samples to be tested is greater than 1, the control module is further configured to:

When the single specific protein measurement of the current blood sample to be detected is not completed, the specific protein detection module which is not in the detection state is called to measure the content of the corresponding single specific protein in the next blood sample to be detected until all the blood samples to be detected complete the single specific protein measurement.

Preferably, the specific proteins include CRP and HbA1 c.

preferably, the blood routine measurement mode comprises a CBC blood cell three classification measurement mode and a CBC blood cell five classification measurement mode; the blood routine detection module comprises a WBC detection module, an RBC detection module, an HGB detection module and a DIFF detection module;

correspondingly, the control module is specifically configured to:

When a CBC blood cell three-classification measurement mode is selected from the mode selection module, calling the WBC detection module to carry out three-classification and counting on white blood cells in the current blood sample to be detected; calling the RBC detection module to detect red blood cells and platelets in the blood sample to be detected; calling the HGB detection module to detect the concentration of hemoglobin in the current blood sample to be detected;

When the CBC blood cell five-classification measurement mode is selected from the mode selection module, calling the WBC detection module to classify and count basophils in the current blood sample to be detected; calling the RBC detection module to detect red blood cells and platelets in the blood sample to be detected; calling the HGB detection module to detect the concentration of hemoglobin in the current blood sample to be detected; and calling the DIFF detection module to perform four classification and counting on the white blood cells in the blood sample to be detected currently.

Preferably, when the specific protein comprises CRP, the control module is specifically configured to:

Calling a specific protein detection module to measure the content of CRP in the blood sample to be detected so as to obtain a CRP content value;

and correcting the CRP content value according to the HCT value detected by the RBC detection module, and taking the corrected CRP content value as the CRP content value in the current blood sample to be detected.

Preferably, each specific protein detection module comprises a reaction cell, a colorimetric cell and a light receiving module;

Correspondingly, when a specific protein detection module is called to measure the content of a target specific protein in the blood sample to be detected, the control module is specifically configured to:

adding the current blood sample to be detected and the reaction reagent corresponding to the target specific protein into the reaction tank for reaction, and adding the reacted mixture into the colorimetric tank;

and projecting the first optical signal to the colorimetric pool by using an optical emission module, and obtaining a second optical signal representing the turbidity of the colorimetric pool by using the optical receiving module so as to measure the content of the target specific protein by using an immunoturbidimetry.

preferably, a plurality of the specific protein detection modules share the same light emitting module.

The invention provides a blood analyzer, which can realize the joint detection of blood routine and multiple specific proteins, and because the blood analyzer is provided with multiple specific protein detection modules which are independent from each other and have the same structure, the parallel measurement can be realized by different specific protein detection modules when the content of the multiple specific proteins in a blood sample is measured.

Drawings

in order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed in the prior art and the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

Fig. 1 is a schematic structural diagram of a blood analyzer according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of a specific protein detection module according to an embodiment of the present invention;

Fig. 3 is a flowchart of a CRP measurement mode according to an embodiment of the present invention;

FIG. 4 is a flowchart of a HbA1c measurement mode according to an embodiment of the present invention.

Detailed Description

The core of the invention is to provide a blood analyzer, which can realize parallel measurement by different specific protein detection modules when measuring the content of various specific proteins in a blood sample, thereby shortening the detection time and meeting the requirement of emergency detection.

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The blood analyzer of the present application has a blood routine detection module and two or more specific protein detection modules. The blood routine detection module is used for judging the blood condition and checking the disease condition by observing the number change and morphological distribution of blood cells in the blood of a human body; the specific protein detection module is used for detecting disease conditions by measuring the content of specific protein in human blood. It should be noted that the specific protein detection modules herein have the same structure, i.e., the same function, and operate independently of each other, so that a plurality of specific protein detection modules can operate simultaneously.

specifically, the blood routine detection module includes: a WBC (white blood cell) detection module for tri-classifying (lymphocytes, intermediate cell populations, and neutrophils) and counting leukocytes in a blood sample; a Red Blood Cell (RBC) detection module for detecting the number and morphology of red blood cells and platelets in a blood sample; an HGB (Hemoglobin) detection module for detecting a concentration of Hemoglobin in a blood sample; DIFF detection module for four classification (neutrophils, monocytes, lymphocytes and eosinophils) and enumeration of leukocytes in blood samples.

On this basis, please refer to fig. 1, fig. 1 is a schematic structural diagram of a blood analyzer according to an embodiment of the present invention. This blood analyzer includes:

the mode selection module 01 is used for selecting one or more target measurement modes from the measurement mode set to carry out measurement; a measurement module 02 including a blood routine detection module and a plurality of specific protein detection modules; the control module 03 is configured to invoke the corresponding detection module to detect the blood sample to be detected when one or more target measurement modes are selected from the mode selection module 01.

specifically, the set of measurement modes may include: blood routine measurement mode, multiple specific protein measurement mode, single specific protein measurement mode. Among them, the Blood routine measurement mode includes a CBC (complete Blood Count) three-class measurement mode and a CBC five-class measurement mode.

each measurement mode in the measurement mode set can be selected to perform measurement, and the measurement principle of each measurement mode is described as follows:

1) CBC blood cell three-class measurement mode: when a CBC blood cell three-classification measurement mode is selected from the mode selection module 01, collecting the current blood sample to be detected, and distributing the current blood sample to be detected to a WBC detection module, a RBC detection module and an HGB detection module one by one; and respectively calling the WBC detection module, the RBC detection module and the HGB detection module to realize corresponding detection functions.

2) CBC blood cell five classification measurement mode: when the CBC blood cell five-classification measurement mode is selected from the mode selection module 01, collecting the current blood sample to be detected, and distributing the current blood sample to be detected to the WBC detection module, the RBC detection module, the HGB detection module and the DIFF detection module one by one; respectively calling a WBC detection module, an RBC detection module, an HGB detection module and a DIFF detection module to realize corresponding detection functions; among them, the five classes of leukocytes include neutrophils, monocytes, lymphocytes, eosinophils, and basophils.

3) Various specific protein measurement modes: when M specific protein measurement modes are selected from the mode selection module 01, collecting a current blood sample to be detected, selecting M specific protein detection modules from the N specific protein detection modules, and distributing the current blood sample to be detected for the M specific protein detection modules one by one; and respectively calling the selected M specific protein detection modules, enabling each specific protein detection module to measure the content of one specific protein in the M specific proteins in the blood sample to be detected, and enabling different specific protein detection modules not to repeatedly measure the same specific protein, thereby realizing the parallel measurement of the M specific proteins (M is less than or equal to N).

for example, when a specific protein measurement mode of two specific proteins, namely, a specific protein a + a specific protein B, is selected from the mode selection module 01, a blood sample to be currently detected is collected, two specific protein detection modules, namely, the specific protein a detection module and the specific protein B detection module, are selected from the N specific protein detection modules, and the two specific protein detection modules are allocated with the blood sample to be currently detected one by one; the specific protein A detection module is called to measure the content of the specific protein A in the blood sample to be detected, and the specific protein B detection module is called to measure the content of the specific protein B in the blood sample to be detected, so that the parallel measurement of the specific protein A and the specific protein B is realized, and the detection time is shortened.

4) Single specific protein measurement mode: when a single specific protein measurement mode is selected from the mode selection module 01, collecting a current blood sample to be detected, selecting a specific protein detection module which is not in a detection state from the specific protein detection modules, wherein the specific protein detection module is called a first target specific protein detection module, and distributing the current blood sample to be detected to the first target specific protein detection module; and calling a first target specific protein detection module to measure the content of a single specific protein in the blood sample to be detected currently.

The measurement modes in the measurement mode set can also be selected in combination for measurement, such as a blood routine measurement mode + a plurality of specific protein measurement modes, a blood routine measurement mode + a single specific protein measurement mode, and the like. It should be noted that, since a single specific protein measurement mode is to select a specific protein from a specific protein list, and a plurality of specific protein measurement modes are to select a plurality of specific proteins from a specific protein list, these two modes cannot be selected in combination.

Specifically, the specific proteins in the specific protein list include proteins such as CRP and HbA1 c. CRP is an acute phase protein synthesized by stem cells when a human body is subjected to inflammatory stimulation such as microbial invasion or tissue injury. The clinical application of CRP detection is quite wide, including diagnosis and identification of acute infectious diseases, monitoring of infection after surgery, observation of antibiotic curative effect and the like, so that the CRP detection has important clinical value. HbA1c is a product of the binding of hemoglobin in red blood cells in human blood to blood glucose. The combination of blood sugar and hemoglobin to generate HbA1c is irreversible reaction and is in direct proportion to blood sugar concentration, and keeps about 120 days without obvious relation with temporary blood sugar fluctuation, so that the HbA1c can objectively and accurately reflect the average blood sugar level of a patient within 120 days before blood sampling.

for the measurement of the content of the CRP, considering that a certain deviation exists between the measured CRP content value and the content value of the CRP actually contained in the current blood sample to be detected, and considering that the content value of the CRP actually contained in the current blood sample to be detected has a certain correlation with the Hematocrit (HCT) in the current blood sample to be detected, after the CRP content value in the current blood sample to be detected is obtained, the CRP content value is corrected according to the HCT value in the current blood sample to be detected by the RBC detection module, and the corrected CRP content value is used as the CRP content value in the current blood sample to be detected, so that the CRP content value is measured more accurately.

in addition, it is considered that if the total number of the specific protein detection modules is greater than the number of the specific proteins measured by the blood sample to be detected, when the specific protein measurement is performed only on the current blood sample to be detected, there is a specific protein detection module which is not in a detection state, that is, an idle specific protein detection module; moreover, the measurement time of different specific proteins is different, when the measurement of the first specific protein in the blood sample to be detected is completed, and the measurement of the second specific protein is not completed, the specific protein detection module for measuring the first specific protein is in an idle state.

based on this, when the number of blood samples to be detected is greater than 1, the measurement principle of the plurality of specific protein measurement modes and the single specific protein measurement mode is explained:

1) Single specific protein measurement mode: when the single specific protein measurement of the current blood sample to be detected is not completed, the next blood sample to be detected is automatically collected, one specific protein detection module which is not in a detection state is selected from the specific protein detection modules and is called as a second target specific protein detection module, the next blood sample to be detected is distributed to the second target specific protein detection module, and then the second target specific protein detection module is called to measure the content of the single specific protein in the next blood sample to be detected until all the blood samples to be detected complete the single specific protein measurement. Therefore, the content of the same specific protein in different blood samples can be measured in parallel, the detection time is shortened, and the single specific protein measurement efficiency of a plurality of blood samples to be detected is improved.

2) Various specific protein measurement modes: when the measurement of various specific proteins in the current blood sample to be detected is not finished, the specific protein detection module which is not in a detection state is called to measure the content of the specific proteins in the next blood sample to be detected one by one until all the blood samples to be detected are measured by the various specific proteins, so that the idle specific protein detection module is used for reducing the specific protein measurement time of the blood samples to be detected, and further the specific protein measurement efficiency of the blood samples to be detected is improved.

Preferably, the same specific protein of different blood samples to be detected of the present application calls the same specific protein detection module for detection, in order to avoid detection residue interference between different specific proteins.

For example, if the number of the blood samples to be detected is greater than 1, when the CBC blood cell five-class measurement mode + CRP specific protein measurement mode is selected from the mode selection module 01, the present application may invoke any idle specific protein detection module to complete specific protein measurement of the next blood sample to be detected.

for another example, if the number of the blood samples to be detected is greater than 1, the CBC blood cell five classification measurement mode + CRP specific protein measurement mode + HbA1c specific protein measurement mode is selected from the mode selection module 01, and the application calls the specific protein detection module to operate according to the number of the specific protein detection modules. Specifically, if the number of the specific protein detection modules is 2, 1 specific protein detection module is allocated to each of the two proteins of CRP and HbA1c for specific protein measurement; if the number of the specific protein detection modules is 3, 2 specific protein detection modules are allocated to the specific protein with low detection speed, 1 specific protein detection module is allocated to the specific protein with high detection speed (the allocation principle of the specific protein detection modules with more numbers is the same), and the detection of the same specific protein by each specific protein detection module is ensured as much as possible.

In addition, referring to fig. 2, fig. 2 is a schematic structural diagram of a specific protein detection module according to an embodiment of the present invention. Each specific protein detection module includes a reaction cell 101, a colorimetric cell 102, and a light-receiving module 103. When a specific protein detection module is called to measure the content of a target specific protein in a blood sample to be detected, the target specific protein measurement process comprises the following steps: adding the current blood sample to be detected and the reaction reagent corresponding to the target specific protein into a reaction tank 101 for reaction, and adding the reacted mixture into a colorimetric tank 102; the first light signal is projected to the colorimetric pool 102 by the light emitting module, and the second light signal representing the turbidity of the colorimetric pool 102 is obtained by the light receiving module 103, so as to measure the content of the target specific protein by the immunoturbidimetry.

specifically, the reaction cell 101 is a place where a blood sample and a reaction reagent react, the colorimetric cell 102 is a place where a mixture after reaction is subjected to specific protein content measurement, and the light receiving module 103 is a module used in cooperation with the light emitting module, and the working principle thereof is as follows: the light emitting module projects a first light signal to the cuvette 102 and the light receiving module 103 receives a second light signal that can characterize the turbidity of the cuvette 102 by transmission or scattering, thereby enabling specific protein measurement using immunoturbidimetry. For example, referring to fig. 3 and fig. 4, fig. 3 is a flowchart of a CRP measurement mode according to an embodiment of the present invention, and fig. 4 is a flowchart of an HbA1c measurement mode according to an embodiment of the present invention.

More specifically, the basic principle of immunoturbidimetry is: when the antigen and the antibody react in a special dilution system and the proportion is proper, the formed soluble immune complex is separated out from the liquid phase to form particles under the action of the polymerization promoter in the dilution system, so that the reaction liquid has turbidity. When the antibody concentration is fixed, the amount of the formed immunocomplex increases with the increase in the amount of the antigen in the test sample, and the turbidity of the reaction solution also increases. The content of the antigen in the detection sample can be calculated by measuring the turbidity of the reaction solution and comparing with a series of standard products.

In addition, a plurality of specific protein detection modules of the application can share the same light emitting module, so that the cost is saved.

In summary, the present application provides a blood analyzer, which can realize the combined detection of blood routine + multiple specific proteins or blood routine + single specific protein. For the combined detection of blood routine and multiple specific proteins, in the prior art, a blood analyzer is only provided with a set of specific protein detection instruments, and when a patient needs to simultaneously measure the content of multiple specific proteins in blood, the same specific protein detection instrument is usually adopted to successively detect blood samples of the patient for multiple times so as to respectively measure the content of multiple specific proteins in the blood. However, the method of sequentially detecting blood samples results in a long detection time, and thus the requirement of emergency detection cannot be met. The blood analyzer is provided with a plurality of specific protein detection modules which are independent mutually but have the same structure, so when the content of a plurality of specific proteins in a blood sample is measured, the contents of the plurality of specific proteins in the blood sample can be measured one by one through different specific protein detection modules, thereby realizing the parallel measurement of the plurality of specific proteins, shortening the detection time and meeting the requirement of emergency detection.

it is further noted that, in the present specification, relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.