阅读说明：本技术 通过适体调节的rna酶p切割调控基因表达 (RNAse P cleavage regulated Gene expression by aptamer modulation ) 是由 郭雪翠 于 2018-03-02 设计创作，主要内容包括：本公开提供了用于通过靶基因RNA的适体介导的核糖核酸酶切割来调节靶基因表达的多核苷酸构建体,以及使用所述构建体来响应于结合适体的配体的存在或不存在来调节基因表达的方法。所述多核苷酸构建体含有核糖核酸酶底物序列(例如,RNA酶P底物)和包含效应物区和适体的核糖开关,使得当适体结合配体时,发生靶基因表达。(The present disclosure provides polynucleotide constructs for modulating expression of a target gene by aptamer-mediated ribonuclease cleavage of the target gene RNA, and methods of using the constructs to modulate gene expression in response to the presence or absence of a ligand that binds to the aptamer. The polynucleotide construct contains a ribonuclease substrate sequence (e.g., an rnase P substrate) and a riboswitch comprising an effector region and an aptamer such that when the aptamer binds to the ligand, target gene expression occurs.)