阅读说明：本技术 一种抗衰老黄芩茶的制备方法及用途 (Preparation method and application of anti-aging scutellaria baicalensis tea ) 是由 周玉枝 冯雪 秦雪梅 张丽增 杜冠华 于 2019-09-03 设计创作，主要内容包括：本发明属于保健食品技术领域,为了解决目前市场上黄芩茶口感、色泽与功效等都无法得到固定,效果不理想的问题,提供了一种抗衰老黄芩茶的制备方法及用途。以5-8月份采摘的黄芩叶为原料进行抽风失水、杀青、揉捻和干燥,制备抗衰老黄芩茶。并采用LC-MS的方法对黄芩茶及其原料黄芩叶进行成分比较,找到9个最主要的差异成分且在黄芩茶中含量高。所制备的黄芩茶能够延缓衰老,原料成本低廉易获得,无毒副作用,黄芩茶制备工艺简单、安全性高,具有显著的保健功效。有很好的市场开发应用前景。(The invention belongs to the technical field of health-care food, and provides a preparation method and application of anti-aging scutellaria baicalensis tea in order to solve the problems that the taste, color, efficacy and the like of scutellaria baicalensis tea in the current market cannot be fixed and the effect is not ideal. The anti-aging scutellaria baicalensis tea is prepared by taking scutellaria baicalensis leaves picked in 5-8 months as raw materials and performing air draft dehydration, enzyme deactivation, rolling and drying. And the LC-MS method is adopted to compare the components of the scutellaria tea and the raw material scutellaria leaf thereof, and 9 most main different components are found and the content of the components in the scutellaria tea is high. The prepared scutellaria tea can delay senility, the raw materials are low in cost and easy to obtain, toxic and side effects are avoided, the preparation process of the scutellaria tea is simple, the safety is high, and the scutellaria tea has a remarkable health-care effect. Has good market development and application prospect.)

1. An anti-aging scutellaria baicalensis tea is characterized in that: the anti-aging scutellaria baicalensis tea is prepared by taking scutellaria baicalensis leaves picked in 5-8 months as raw materials and performing air draft dehydration, enzyme deactivation, rolling and drying.

2. The anti-aging scutellaria baicalensis tea according to claim 1, which is characterized in that: the preparation method comprises the following steps:

(1) picking: selecting 5-8 months old scutellaria leaves without plant diseases and insect pests, and removing impurities to be used as raw materials for later use;

(2) air draft dehydration, treating the selected Scutellariae radix leaves with air dryer at 110 deg.C ~ 150 deg.C for 3 ~ 5min, and deactivating enzyme;

(3) de-enzyming: carrying out 700W microwave enzyme deactivation on the scutellaria baicalensis leaves obtained in the step (2), wherein the enzyme deactivation time is 60s, and then carrying out color protection;

(4) rolling, namely rolling the scutellaria baicalensis leaves obtained in the step (3) by using a 45-type rolling machine, firstly performing air compression for 3 ~ 5min, then performing light compression for 20 ~ 25min, then performing heavy compression for 5 ~ 10min, finally performing loose compression for 2min, and then drying;

(5) and (4) drying the dried scutellaria baicalensis obtained in the step (4) by adopting microwave 700W for 10 ~ 20s, thus obtaining the anti-aging scutellaria baicalensis tea.

3. The method for detecting and analyzing anti-aging active ingredients in anti-aging scutellaria baicalensis tea according to claim 1 or 2, which is characterized in that: the components of the scutellaria tea water extract and the chemical components of the naturally dried scutellaria leaf water extract are analyzed by LC-MS, so that 9 main different components are obtained: the content of carthamin-7-O-glucuronide, baicalin, wogonoside, isocarthamin-7-O-glucuronide, isobaicalin-7-O-glucuronide, baicalein, scutellarin, chrysin-7-O-glucuronide and 5, 6, 7, 3', 4' pentahydroxyflavanone-7-O-glucuronide in the scutellaria tea is respectively higher than that in the scutellaria tea.

4. The use of the anti-aging scutellaria baicalensis tea of claim 1 or 2, which is characterized in that: the anti-aging scutellaria baicalensis tea can obviously prolong the average life, median survival time and maximum life of fruit flies, p is less than 0.05, and the anti-aging scutellaria baicalensis tea is applied to prolonging the life and delaying aging.

5. The method for detecting and analyzing anti-aging active ingredients in anti-aging scutellaria baicalensis tea according to claim 3, characterized in that: the specific method comprises the following steps:

(1) obtaining the scutellaria tea water extract and the scutellaria leaf water extract: scutellaria baicalensis tea water extract: weighing Scutellariae radix tea, and extracting at 80-100 deg.C twice; adding water 15 times the weight of Scutellariae radix tea for the first time, extracting for 1 hr, filtering, adding water 10 times the weight of Scutellariae radix tea for the second time, extracting for 1 hr, filtering, mixing filtrates, and concentrating with rotary evaporator; vacuum freeze-drying the obtained concentrated solution to obtain Scutellariae radix tea extract;

(2) aqueous extract of scutellaria baicalensis leaves: weighing Scutellariae radix leaves, and extracting at 89 deg.C twice; adding water 15 times the weight of the folium Scutellariae for the first time, extracting for 1h, filtering, adding water 10 times the weight of the folium Scutellariae for the second time, extracting for 1h, filtering, mixing filtrates, and concentrating with rotary evaporator; vacuum freeze-drying the obtained concentrated solution to obtain Scutellariae radix leaf extract;

(2) analyzing the components of the scutellaria tea water extract and the scutellaria leaf water extract by LC-MS:

sample preparation: accurately weighing Scutellariae radix tea water extract and Scutellariae radix leaf water extract 150 mg respectively, placing into conical flask with plug, adding 70% ethanol 50 mL, weighing, ultrasonic extracting at 80 deg.C for 1h, weighing, supplementing with 70% ethanol, reducing weight loss, standing, and filtering; filtering the filtrate with 0.22 μm microporous membrane;

all detection samples are parallelly prepared into 6 parts; the QC (quality control) sample is formed by mixing equal amounts of all samples to be detected; after the sample introduction of each 8-pin sample is finished, the QC sample is subjected to sample introduction once, and the stability of an instrument is detected;

and comparing the total ion flow diagrams of the scutellaria tea water extract and the scutellaria leaf water extract to obtain different components through multivariate statistics.

6. Use of the scutellaria tea of claim 1 or 2 for the preparation of an anti-aging medicament.

7. Use of the scutellaria tea of claim 1 or 2 for the preparation of an anti-aging health product.

Technical Field

The invention belongs to the technical field of health-care food, and particularly relates to a preparation method and application of anti-aging scutellaria baicalensis tea

Background

Aging is a degenerative change in the function of tissues and organs of the body with age, and is the result of the combined action of many factors in vitro and in vivo. At present, China faces the development trend of large total population and rapid acceleration of the elderly, and the health of the elderly, the prevention and control of chronic diseases and the like become the most prominent problems in the current society. With the continuous development of Chinese medicine theory and the improvement of health consciousness of people, anti-aging is more and more concerned by people, people begin to develop beverages or health products for conditioning the body to achieve the effects of health care, prevention and treatment of diseases. Therefore, the research and development of the traditional Chinese medicine health food capable of delaying senility and improving memory dysfunction caused by senility have important significance.

The Scutellariae radix tea is prepared from Scutellariae radix (Scutellaria baicalensis Georgi) of Scutellaria of LabiataeScutellaria baicalensis Georgi) The leaves of the scutellaria baicalensis is harvested in 5 ~ 8 months and processed, researchers partially study chemical components and pharmacological effects of the scutellaria baicalensis leaves, wherein the scutellaria baicalensis leaves mainly contain flavonoids, organic acids, diterpenes, volatile oils, polysaccharides, inorganic elements and the like in the aspect of chemical components, and the scutellaria baicalensis leaves have various pharmacological activities of myocardial ischemia-reperfusion injury protection, cardiovascular protection, liver protection, antipyretic and analgesic effects, antibacterial effects, antiviral effects, anti-inflammatory effects, antioxidation effects, immunity enhancement effects, central nervous protection effects, blood sugar reduction effects, blood fat reduction effects, anti-tumor effects and the like in the aspect of pharmacological effects.

The Shanxi Baikal skullcap root has rich resources, accounts for more than 40 percent of the national yield, and the Baikal skullcap root is a perennial root herb, so the Baikal skullcap root tea raw material, namely the Baikal skullcap root leaves, has a considerable reserve. At present, most of the scutellaria baicalensis tea on the market is prepared by mixing overground parts, and the taste, color and effect are not ideal.

Disclosure of Invention

The invention provides anti-aging scutellaria baicalensis tea, a preparation method thereof and an anti-aging active ingredient detection and analysis method, aiming at solving the problems that the taste, color, efficacy and the like of scutellaria baicalensis tea in the current market cannot be fixed and the effect is not ideal. The anti-aging scutellaria baicalensis tea is prepared by taking scutellaria baicalensis leaves as raw materials, and the components of the scutellaria baicalensis tea and the scutellaria baicalensis leaves (naturally dried in the shade) which are used as the raw materials of the scutellaria baicalensis tea are compared by adopting an LC-MS method, so that the 9 most main different components are found, and the content of the components in the scutellaria baicalensis tea is high. The prepared scutellaria tea can delay senility, the raw materials are low in cost and easy to obtain, toxic and side effects are avoided, the preparation process of the scutellaria tea is simple, the safety is high, and the scutellaria tea has a remarkable health-care effect.

In order to achieve the purpose, the invention adopts the following technical scheme:

an antiaging Scutellariae radix tea is prepared from Scutellariae radix leaves picked in 5-8 months by air draft dehydration, enzyme deactivation, rolling and drying.

The preparation method comprises the following steps:

(1) picking: selecting 5-8 months old scutellaria leaves without plant diseases and insect pests, and removing impurities to be used as raw materials for later use;

(2) air draft dehydration, treating the selected Scutellariae radix leaves with air dryer at 110 deg.C ~ 150 deg.C for 3 ~ 5min, and deactivating enzyme;

(3) de-enzyming: carrying out 700W microwave enzyme deactivation on the scutellaria baicalensis leaves obtained in the step (2), wherein the enzyme deactivation time is 60s, and then carrying out color protection;

(4) rolling, namely rolling the scutellaria baicalensis leaves obtained in the step (3) by using a 45-type rolling machine, firstly performing air compression for 3 ~ 5min, then performing light compression for 20 ~ 25min, then performing heavy compression for 5 ~ 10min, finally performing loose compression for 2min, and then drying;

(5) and (4) drying the dried scutellaria baicalensis obtained in the step (4) by adopting microwave 700W for 10 ~ 20s, thus obtaining the anti-aging scutellaria baicalensis tea.

The method for detecting and analyzing the anti-aging active ingredients in the anti-aging scutellaria baicalensis tea comprises the following steps of carrying out ingredient analysis on chemical ingredients in a scutellaria baicalensis tea water extract and a scutellaria baicalensis leaf water extract through LC-MS to obtain 9 main different ingredients: the content of carthamin-7-O-glucuronide, baicalin, wogonoside, isocarthamin-7-O-glucuronide, isobaicalin-7-O-glucuronide, baicalein, scutellarin, chrysin-7-O-glucuronide and 5, 6, 7, 3', 4' pentahydroxyflavanone-7-O-glucuronide in the scutellaria tea is respectively higher than that in the scutellaria tea.

The anti-aging scutellaria baicalensis tea can obviously prolong the average life, median survival time and maximum life of fruit flies, p is less than 0.05, and the anti-aging scutellaria baicalensis tea is applied to prolonging the life and delaying aging.

The specific analysis method comprises the following steps:

(1) obtaining the scutellaria tea water extract and the scutellaria leaf water extract: scutellaria baicalensis tea water extract: weighing Scutellariae radix tea, and extracting at 89 deg.C twice; adding water 15 times the weight of Scutellariae radix tea for the first time, extracting for 1 hr, filtering, adding water 10 times the weight of Scutellariae radix tea for the second time, extracting for 1 hr, filtering, mixing filtrates, and concentrating with rotary evaporator; vacuum freeze-drying the obtained concentrated solution to obtain Scutellariae radix tea extract;

aqueous extract of scutellaria baicalensis leaves: weighing Scutellariae radix leaves, and extracting at 80-100 deg.C twice; adding water 15 times the weight of the folium Scutellariae for the first time, extracting for 1h, filtering, adding water 10 times the weight of the folium Scutellariae for the second time, extracting for 1h, filtering, mixing filtrates, and concentrating with rotary evaporator; vacuum freeze-drying the obtained concentrated solution to obtain Scutellariae radix leaf extract;

(2) analyzing the components of the scutellaria tea water extract and the scutellaria leaf water extract by LC-MS:

sample preparation: accurately weighing Scutellariae radix tea water extract and Scutellariae radix leaf water extract 150 mg respectively, placing into conical flask with plug, adding 70% ethanol 50 mL, weighing, ultrasonic extracting at 80 deg.C for 1h, weighing, supplementing with 70% ethanol, reducing weight loss, standing, and filtering; filtering the filtrate with 0.22 μm microporous membrane;

all detection samples are parallelly prepared into 6 parts; the QC (quality control) sample is formed by mixing equal amounts of all samples to be detected; after the sample introduction of each 8-pin sample is finished, the QC sample is subjected to sample introduction once, and the stability of an instrument is detected;

and comparing the total ion flow diagrams of the scutellaria tea water extract and the scutellaria leaf water extract to obtain different components through multivariate statistics.

The chromatographic conditions of the LC-MS are as follows: mobile phase: a is acetonitrile, B is 0.1% aqueous formic acid, gradient elution procedure: 0-3 min, 15% -23% A; 3-7 min, 23% -25% A; 7-9 min, 25% -27% A; 9-11 min, 27% -50% A; 11-14 min, 50% -52% A; 14-15 min, 52% -90% A; 15-17 min, 90% A; 17-17.5 min, 90% -15% A; 17.5-20 min, 15% A; the flow rate is 0.30mL min < -1 >, the column temperature is 40 ℃, the temperature of the sample pool is 15 ℃, and the sample injection volume is 3 mu L;

the mass spectrum condition adopts ESI positive and negative ion detection mode, the atomization gas is N2, the collision gas is He, the mass spectrum full scan and secondary scan are adopted, the mass scan range is 100 ~ 1500 m/z, the capillary voltage under the positive and negative ion mode is 3.5KV and 2.5KV respectively, the desolvation gas temperature is 300 ℃, and the capillary temperature is 320 ℃.

Through detection, compared with a control group, the prepared scutellaria baicalensis tea can obviously improve the life condition of the fruit flies (p is less than 0.01), can obviously improve the average life, the median life and the maximum life of the fruit flies (p is less than 0.05), and has an anti-aging effect.

The application of the scutellaria tea in preparing anti-aging medicaments. The application of the scutellaria tea in preparing an anti-aging health product.

The invention adopts natural traditional Chinese medicines as raw materials, more exactly selects the scutellaria baicalensis leaves as the raw materials for preparing the scutellaria baicalensis tea, has low cost, easy obtainment and no toxic or side effect, has simple and easy operation of the preparation process of the scutellaria baicalensis tea, and has difference in the content of the scutellaria baicalensis leaves of the raw materials of the scutellaria baicalensis tea. The prepared scutellaria tea can obviously prolong the life of fruit flies and has definite effect. Fully shows that the anti-aging scutellaria baicalensis tea prepared by the invention can fully play an anti-aging role.

Drawings

FIG. 1 shows UHPLC-ESI of aqueous extract of Scutellaria baicalensis Georgi and aqueous extract of Scutellaria baicalensis Georgi^-MS/MS total ion flow diagram, in which: a is water extract of Scutellariae radix leaf; b is Scutellariae radix tea water extract;

FIG. 2 is a multivariate statistical analysis of chemical components of different growing parts of Scutellaria baicalensis Georgi; in the figure: a is PCA Scatter plot; b is PLS-DA Scatter plot; c is PLS-DA permatation test; d is S-plot;

FIG. 3 shows the relative contents of different components of the aqueous extract of Scutellaria baicalensis Georgi and the aqueous extract of Scutellaria baicalensis Georgi (n = 6);

FIG. 4 is a graph of life span of male Drosophila in a model of natural senescence.

Detailed Description

The present invention is further described with reference to specific embodiments, so that the objects, technical solutions and advantages of the present invention are more clearly understood. These examples are only illustrative and are not intended to limit the scope of the invention.