阅读说明：本技术 基于带病毒昆虫残体的氨基酸水解液及其制备方法和应用 (Amino acid hydrolysate based on insect residues with viruses and preparation method and application thereof ) 是由 尹涛 陈娇 林春鸿 高琪 陶佩文 于 2019-10-21 设计创作，主要内容包括：本发明提供了一种基于带病毒昆虫残体的氨基酸水解液,其由按质量百分比计的如下组分制备得到：带病毒昆虫残体40～85%、稀酸液10～60%、稀碱液0～15%。该发明的原材料来源广泛并易于获得,而且昆虫病毒是纯度较高的蛋白质,其它种类的有机组分含量较低,水解后的氨基酸液中糖类、脂类等成分含量较低,对深加工过程涉及的提纯和除杂工段造成的影响较小。本发明还提供了基于带病毒昆虫残体的氨基酸水解液的制备方法,利用化学水解的方法将带病毒昆虫残体进行再利用,转化成新的产品,作为其它领域的生产原料,既节约了资源,提高了病毒工业本身的效益,也促进其它工业领域的发展,具有良好的市场应用前景。(The invention provides an amino acid hydrolysate based on virus-carrying insect residues, which is prepared from the following components in percentage by mass: 40-85% of virus-carrying insect residues, 10-60% of dilute acid solution and 0-15% of dilute alkali solution. The raw materials of the invention are widely available and easy to obtain, the insect virus is protein with higher purity, the content of other kinds of organic components is lower, the content of components such as carbohydrate, lipid and the like in the amino acid solution after hydrolysis is lower, and the influence on the purification and impurity removal working sections involved in the deep processing process is smaller. The invention also provides a preparation method of the amino acid hydrolysate based on the insect residues with the viruses, and the insect residues with the viruses are recycled by using a chemical hydrolysis method and are converted into new products to be used as production raw materials in other fields, so that the resources are saved, the benefits of the virus industry are improved, the development of other industrial fields is promoted, and the amino acid hydrolysate has good market application prospects.)

1. The amino acid hydrolysate based on the virus-carrying insect residues is characterized by being prepared from the following components in percentage by mass: 40-85% of virus-carrying insect residues, 10-60% of dilute acid solution and 0-15% of dilute alkali solution.

2. The amino acid hydrolysate based on virus-bearing insect residues according to claim 1, wherein the virus-bearing insect residues are at least one of Blattaria insect residues, Lepidoptera insect residues, and Coleoptera insect residues.

3. The amino acid hydrolysate based on viral insect residues according to claim 1, wherein the dilute acid solution is at least one of a dilute hydrochloric acid solution, a dilute sulfuric acid solution, a dilute acetic acid solution, and a dilute nitric acid solution.

4. The amino acid hydrolysate solution based on residual bodies of viral insects as set forth in claim 1, wherein the dilute alkali solution is at least one of a dilute sodium hydroxide solution, a dilute potassium hydroxide solution, a dilute sodium carbonate solution, a dilute sodium bicarbonate solution, a dilute potassium carbonate solution, and a dilute potassium bicarbonate solution.

5. The method for preparing the amino acid hydrolysate based on the residual bodies of the virus-bearing insects as claimed in any one of claims 1 to 4, which comprises the following steps:

1) pretreatment of insect residues with virus

Stirring and cleaning the insect residues with the viruses in a cleaning solution, filtering the cleaned insect residues with the viruses by two stages to remove impurities, separating the cleaning solution, drying the insect residues with the viruses after cleaning and filtering, and crushing the dried insect residues with the viruses into uniform powder;

hydrolysis

Adding the powdery insect residues with the viruses into a dilute acid solution for hydrolysis reaction;

neutralization

And neutralizing the hydrolyzed reaction liquid with dilute alkali liquor to be weakly acidic or neutral, and filtering the neutralized reaction liquid to obtain filtrate, namely the amino acid hydrolysate.

6. The method for preparing the amino acid hydrolysate based on the residual bodies of the insects with the viruses as claimed in claim 5, wherein the cleaning solution in the step 1) is tap water, the cleaning temperature is 20-30 ℃, and the cleaning time is 20 min; the two-stage filtration comprises coarse filtration for removing impurities in the residual bodies of the insects with the viruses and fine filtration for separating the residual bodies of the insects with the viruses from cleaning fluid; the drying medium used for drying the virus-carrying insect residues is hot air, the temperature of the hot air is 50-60 ℃, and the drying process lasts for 25-30 min.

7. The method of claim 5, wherein the insect residues with virus are pulverized into a uniform powder with a particle size of 80 mesh or larger.

8. The method for preparing the amino acid hydrolysate based on the residual bodies of the insects with the viruses as claimed in claim 5, wherein the hydrolysis temperature in the step 2) is 70-95 ℃, the hydrolysis time is 10-26 h, and the stirring is continued during the hydrolysis process.

9. The method for preparing the amino acid hydrolysate based on the residual bodies of the insects with the viruses according to claim 5, wherein the pH of the neutralized reaction solution in the step 3) is 6.0-7.0.

10. The use of the amino acid hydrolysate based on virus-containing insect residues according to any one of claims 1 to 4, wherein the amino acid hydrolysate based on virus-containing insect residues is used for preparing an amino acid-containing agricultural fertilizer.

Technical Field

The invention belongs to the technical field of amino acid preparation, and particularly relates to an amino acid hydrolysate based on virus-carrying insect residues, and a preparation method and application thereof.

Background

At present, insect viruses are a mature technology applied to the field of plant protection, and have the characteristics of high targeting property, strong infectivity in insect groups, environmental friendliness and the like, so that the insect viruses play an important role in a green ecological prevention and control system of agricultural insect pests. The species of the plant virus is related to a plurality of species and varieties of cockroaches, noctuids, cabbage caterpillars, pine moth, cotton bollworms, tea geometrids and the like, and the species and the source of the virus are extremely wide and are all taken from the nature.

After the insect virus is separated and purified in industrial production, a large amount of insect disease residues are left, a large amount of virosomes still remain in the insect disease residues, the virosomes comprise various specific amino acids, and most of the specific amino acids can be used for deep processing in the fields of agriculture, industry and even medicine. The fertilizer can be further processed into amino acid functional fertilizer in the agricultural field, and plays a special role in regulating and controlling the growth of crops; can be further purified or further processed into various products in the industrial field; can be used for purifying or further processing into various intermediates in the medical field.

In addition, the insect residues with viruses are main wastes in the insect virus production process, have special unpleasant odor, and can influence the living environment of people on one hand and increase the burden of waste treatment on the other hand if the insect residues are not treated. However, the technology for producing amino acid by recycling insect disease residues in the domestic virus industry is few, and the compatibility of specific amino acid constituting insect virus with the requirements of other industrial fields is not deeply researched mainly because of few mechanisms for relevant research in the domestic insect virus industry.

Disclosure of Invention

The invention aims to solve the problems of environmental pollution caused by the waste of the existing insect residues with viruses and resource waste caused by the increase of waste treatment burden.

Therefore, the invention provides an amino acid hydrolysate based on virus-carrying insect residues, which is prepared from the following components in percentage by mass: 40-85% of virus-carrying insect residues, 10-60% of dilute acid solution and 0-15% of dilute alkali solution.

Further, the virus-bearing insect residues are at least one of Blattaria insect residues, Lepidoptera insect residues and coleoptera insect residues; wherein the insect disease residues of Blattaria include periplaneta americana disease residues, Blattella germanica disease residues, Termite disease residues, and the like, the lepidoptera insect disease residues include Autographa californica disease residues, beet armyworm disease residues, prodenia litura disease residues, cabbage caterpillar disease residues, pine moth disease residues, cotton bollworm disease residues, tea geometrid disease residues, and coleoptera insect disease residues include Typha angustifolia disease residues, scarab beetle disease residues, and the like.

Further, the dilute acid solution is at least one of a dilute hydrochloric acid solution, a dilute sulfuric acid solution, a dilute acetic acid solution and a dilute nitric acid solution.

Further, the dilute alkali solution is at least one of dilute sodium hydroxide solution, dilute potassium hydroxide solution, dilute sodium carbonate solution, dilute sodium bicarbonate solution, dilute potassium carbonate solution and dilute potassium bicarbonate solution.

In addition, the invention also provides a preparation method of the amino acid hydrolysate based on the residual bodies of the virus-carrying insects, which comprises the following steps:

1) pretreatment of insect residues with virus

Stirring and cleaning the insect residues with the viruses in a cleaning solution, filtering the cleaned insect residues with the viruses by two stages to remove impurities, separating the cleaning solution, drying the insect residues with the viruses after cleaning and filtering, and crushing the dried insect residues with the viruses into uniform powder;

2) hydrolysis

Adding the powdery insect residues with the viruses into a dilute acid solution for hydrolysis reaction;

3) neutralization

And neutralizing the hydrolyzed reaction liquid with dilute alkali liquor to be weakly acidic or neutral, and filtering the neutralized reaction liquid to obtain filtrate, namely the amino acid hydrolysate.

Further, the cleaning liquid in the step 1) is tap water, the cleaning temperature is 20-30 ℃, and the cleaning time is 20 min; the two-stage filtration comprises coarse filtration for removing impurities in the residual bodies of the insects with the viruses and fine filtration for separating the residual bodies of the insects with the viruses from cleaning fluid; the drying medium used for drying the virus-carrying insect residues is hot air, the temperature of the hot air is 50-60 ℃, and the drying process lasts for 25-30 min.

Further, the insect residues with the viruses are crushed into uniform powder with the particle size being not less than 80 meshes.

Further, in the step 2), the hydrolysis temperature is 70-95 ℃, the hydrolysis time is 10-26 hours, and the stirring is continued in the hydrolysis process.

Further, the pH value of the reaction liquid after neutralization in the step 3) is 6.0-7.0.

The amino acid hydrolysate based on the insect residues with the viruses provided by the invention can be used for preparing agricultural fertilizers containing amino acids.

Compared with the prior art, the invention has the beneficial effects that:

(1) the insect virus serving as the raw material based on the amino acid hydrolysate with the virus insect residues is natural, and the insect virus is widely available and easily obtained in industrial production by taking insects bred in nature or artificially as the raw material; in addition, the insect virus contains rich specific proteome and rich specific amino acid library, and the insect virus is taken as a raw material and is assisted with other industrial amino acid extraction process technologies, so that amino acid types which are difficult to obtain under the conventional conditions can be obtained; and insect viruses are proteins with higher purity, the content of other organic components is lower, the content of components such as saccharides, lipids and the like in the amino acid solution after hydrolysis is lower, and the influence on purification and impurity removal sections involved in the deep processing process is smaller.

(2) The preparation method of the amino acid hydrolysate based on the virus-containing insect residues is simple to operate, and overcomes the defects that the residual residues after hydrolysis are excessive due to incomplete hydrolysis of the virus-containing insect residues, and the proteolysis efficiency is reduced due to overhigh or overlow acid liquid concentration in the acidolysis process.

(3) The amino acid hydrolysate based on the insect residues with the viruses provided by the invention recycles the insect residues with the viruses by utilizing a chemical hydrolysis method, converts the insect residues into new products, and is used as a production raw material in other fields, so that the resources are saved, the benefits of the virus industry are improved, the development of other industrial fields is promoted, and the amino acid hydrolysate has good market application prospects.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.