阅读说明：本技术 一种生物指示剂辅助破碎培养装置及其使用方法 (Biological indicator assisted crushing culture device and using method thereof ) 是由 郭洪 何文燕 陈春竹 刘圣艳 于 2019-10-12 设计创作，主要内容包括：本发明公开了一种生物指示剂辅助破碎培养装置,包括培养箱体、安装架和破碎器,培养箱体为无色透明箱体,所述安装架设置在培养箱体上方,安装架内设有若干生物指示剂存放单元,所述培养箱体一侧连接有破碎器连接件,破碎器连接件与所述破碎器可拆卸相连。该装置能够有效辅助生物指示剂的破碎,避免出现扎手或培养基倒流的问题,并且能够辅助生物指示剂的培养,避免芽孢管倒置泄露引起的生物安全危害。本发明还公开一种生物指示剂辅助破碎培养装置的使用方法。(The invention discloses a biological indicator auxiliary crushing culture device which comprises a culture box body, an installation frame and a crusher, wherein the culture box body is a colorless transparent box body, the installation frame is arranged above the culture box body, a plurality of biological indicator storage units are arranged in the installation frame, one side of the culture box body is connected with a crusher connecting piece, and the crusher connecting piece is detachably connected with the crusher. The device can effectively assist the breakage of biological indicator, avoids appearing pricking hand or the problem of culture medium refluence to can assist the cultivation of biological indicator, avoid the spore tube to invert and reveal the biosafety harm that arouses. The invention also discloses a use method of the biological indicator assisted disruption culture device.)

1. The utility model provides a supplementary broken culture apparatus of biological indicator, its characterized in that, including cultivateing box (1), mounting bracket (2) and cracker (3), cultivateing box (1) and being colorless transparent box, mounting bracket (2) set up cultivates box (1) top, are equipped with a plurality of biological indicator in mounting bracket (2) and deposit the unit, cultivate box (1) one side and be connected with cracker connecting piece (4), cracker connecting piece (4) with cracker (3) can dismantle the link, cultivate box (1) and be the rectangle box, the rectangle box includes bottom plate and four curb plates (11) that link to each other with the bottom plate, cracker connecting piece (4) set up on curb plate (11) of cultivateing box (1), cracker (3) include mounting panel (5) and stripper plate (6), be equipped with a spout (51) that runs through mounting panel (5) on mounting panel (5) lateral wall, mounting panel (5) central authorities are equipped with circular shape broken hole (52), and broken hole (52) axis perpendicular to spout (51) place plane, and broken hole (52) are linked together with spout (51), stripper plate (6) are two, and two stripper plate (6) insert spout (51) from spout (51) both ends separately, stripper plate (6) are kept away from spout (51) one end and still are connected with handle (61).

2. The biological indicator assisted disruption culture apparatus according to claim 1, wherein the mounting frame (2) comprises a frame (21) and a plurality of partition plates (22), the frame (21) is rectangular, the rectangular frame (21) is mounted on the top surfaces of four side plates (11) of the culture box body (1), the plurality of partition plates (22) comprise transverse plates and longitudinal plates, the transverse plates and the longitudinal plates are vertically staggered, two ends of the partition plates (22) are fixed on the inner wall of the frame (21), and the partition plates (22) divide the inner space of the frame (21) into a plurality of biological indicator storage units.

3. The biological indicator assisted disruption culture apparatus according to claim 2, wherein the biological indicator storage unit is a culture chamber (23), the inner walls of the frame (21) and the partition plate (22) which enclose the culture chamber (23) are provided with short shafts (24), and the space enclosed by the short shafts (24) is used for placing the biological indicator tube (7).

4. The biological indicator assisted disruption culture apparatus according to claim 1, wherein the mounting plate (5) is a rectangular plate, two sides of the rectangular plate far away from the sliding groove (51) are connected with a round rod (53), and the round rod (53) is parallel to the sliding direction of the extrusion plate (6).

5. The biological indicator assisted disruption culture apparatus of claim 1, wherein the end of the squeezing plate (6) far away from the handle (61) is provided with a circular through hole (62), and the diameters of the circular through hole (62) and the disruption hole (52) are not smaller than the diameter of the biological indicator tube body (7).

6. The biological indicator assisted disruption culture apparatus according to claim 5, wherein two squeezing plates (6) are stacked in a sliding groove (51), the inner wall of the sliding groove (51) is provided with a plurality of limiting grooves (54), and the squeezing plates (6) are provided with a plurality of sliding blocks (63) matched with the limiting grooves (54).

7. The biological indicator assisted disruption culture apparatus of claim 6, wherein the two squeezing plates (6) are moved synchronously, when the squeezing plates (6) slide until the circular through holes (62) coincide with the disruption holes (52) or the circular through holes (62) are positioned at the center of the disruption holes (52), the circular through holes (62) of the two squeezing plates (6) coincide with each other, and the squeezing plates (6) are pressed continuously, the circular through holes (62) of the two squeezing plates (6) intersect with each other gradually, and the gap formed between the two squeezing plates is smaller than the diameter of the biological indicator ampoule.

8. The biological indicator assisted disruption culture apparatus according to claim 5, wherein the disruptor connecting piece (4) is a bar fixed on one side of the culture box body (1), the bar is provided with a strip-shaped groove (41) with a circular arc-shaped cross section, the strip-shaped groove (41) penetrates through the bar, the diameter of the strip-shaped groove (41) is matched with that of the round bar (53), the disruptor (3) is connected with the culture box body (1) through the round bar (53) and the strip-shaped groove (41), and the round bar (53) can freely turn over in the strip-shaped groove (41).

9. The method of using a biological indicator assisted disruption culture apparatus of any one of claims 1-8, comprising the steps of:

1) taking out the biological indicator tube body (7) after sterilization, and crushing an ampoule of the biological indicator tube body (7) by using a crusher (3) to enable the culture medium in the ampoule to flow out and to be in contact with the bacillus on the paper sheet;

2) vertically placing a biological indicator tube body (7) in a culture cavity (23) of a mounting frame (2) on a culture box body (1);

3) the culture box body (1) is sent into a special culture room for constant-temperature culture, and is taken out after the culture is finished, and the color change in the biological indicator tube body (7) is observed through a side plate (11) of the culture box body (1), so that the sterilization effect is judged.

10. The use method of the biological indicator assisted disruption culture apparatus of claim 9, wherein: in the step 1), the ampoule crushing method of the biological indicator tube body (7) comprises the following steps: the upper end of the biological indicator tube body (7) is placed in a crushing hole (52) of a mounting plate (5) of the crusher (3), the handle (61) is held by hand, the extrusion plate (6) is pushed inwards, the extrusion plate (6) extrudes the biological indicator tube body (7) in the crushing hole (52), an ampoule in the biological indicator tube body is crushed, and the internal culture medium flows out and contacts with bacillus on paper sheets.

Technical Field

The invention relates to the field of sterilization detection, in particular to a biological indicator auxiliary crushing culture device and a using method thereof.

Background

The bacillus biological indicator can be used for monitoring the high-low temperature disinfection effect, wherein the pressure steam sterilization biological culture indicator is used for monitoring the pressure steam sterilization effect: monitoring the sterilization effect of the exhaust pressure steam at 121 ℃ and the pre-vacuum pressure steam at 132 ℃ (including an exhaust pot and a pre-vacuum pot); the low-temperature biological culture indicator can be used for monitoring the sterilization effects of a plasma furnace, ethylene oxide sterilization and the like, a glass ampoule containing the indicator in the biological indicator is broken to contact with bacillus on a paper sheet, and then the glass ampoule is placed into an incubator at a desired temperature for culture, and reaction bacillus spores are alive or not through the color change of a culture medium, so that the pressure steam sterilization biological monitoring result is judged.

During the pre-treatment process of the culture, the crushing work has great potential safety hazard, namely, the work of crushing the glass ampoule in the biological indicator has great danger. Because the material outside the spore tube is a multi-purpose flexible plastic product, no special crushing device is provided for crushing, the risk that the glass ampoule in the biological indicator penetrates through the plastic product to prick the hand after being crushed by hands is difficult to avoid, or the problems of culture medium backflow in the spore tube caused by the acting force of other non-special foreign objects and the like are difficult to avoid. Because the spore tube is small in size and about 5mm in diameter, the spore tube is not favorable for standing and easy dumping, and the existing bacillus stearothermophilus used for biological indicators, particularly high-pressure steam sterilization, has higher culture temperature and has biological safety hazard caused by inverted leakage of the indicators such as the spore tube and the like.

Disclosure of Invention

In order to solve the technical problems, the invention discloses a biological indicator auxiliary crushing culture device which can effectively assist the crushing of a biological indicator, avoid the problem of pricking hands or backflow of a culture medium, assist the culture of the biological indicator and avoid biosafety hazards caused by inverted leakage of a spore tube.

The invention also discloses a use method of the biological indicator assisted disruption culture device.

The invention is realized by the following technical scheme:

the utility model provides a supplementary broken culture apparatus of biological indicator, is including cultivateing box, mounting bracket and cracker, cultivates the box and is colorless transparent box, the mounting bracket setting is in cultivateing the box top, is equipped with a plurality of biological indicators in the mounting bracket and deposits the unit, it is connected with the cracker connecting piece to cultivate box one side, the cracker connecting piece with the cracker can be dismantled continuously.

According to the invention, the biological indicator storage unit in the mounting rack is used for placing the biological indicator, so that the biological indicator can be kept in a vertical state in the culture process, and biological safety hazards caused by inverted leakage of a spore tube are avoided.

Further, the cultivation box body is a rectangular box body, the rectangular box body comprises a bottom plate and four side plates connected with the bottom plate, and the crusher connecting piece is arranged on the side plates of the cultivation box body.

Further, the mounting bracket includes frame and a plurality of division board, and the frame is the rectangle, and the frame of rectangle is installed at four curb plate top surfaces of cultivation box, and a plurality of division boards include horizontal board and vertical board, and horizontal board and vertical board are crisscross to be set up perpendicularly, and the division board both ends are fixed at the frame inner wall, the division board is for a plurality of biological indicator storage unit with frame inner space separation.

Furthermore, biological indicator deposits the unit and is cultivateed the chamber, and the frame, the division board inner wall that enclose into cultivateing the chamber all are equipped with the minor axis, and the space that several minor axis encloses is used for placing biological indicator body.

In the invention, the frame of the mounting rack is divided into a plurality of biological indicator storage units, namely a culture cavity, by a plurality of partition plates, and a space for the biological indicator tube body to pass through is enclosed among the short shafts arranged on the inner wall of the culture cavity, thus being beneficial to the vertical placement of the biological indicator tube body.

Further, the knapper comprises a mounting plate and two extrusion plates, a sliding groove penetrating through the mounting plate is formed in the side wall of the mounting plate, a circular breaking hole is formed in the center of the mounting plate, the axis of the breaking hole is perpendicular to the plane of the sliding groove, the breaking hole is communicated with the sliding groove, the two extrusion plates are inserted into the sliding groove from the two ends of the sliding groove respectively, and a handle is further connected to one end, far away from the sliding groove, of each extrusion plate.

According to the invention, when the biological indicator ampoule is crushed, the biological indicator tube body is placed in the crushing hole of the mounting plate, then the handle is held by hand to push the extrusion plate inwards, the extrusion plate extrudes the biological indicator tube body in the crushing hole and crushes the ampoule in the crushing hole, the biological indicator does not need to be received by hand in the whole process, the risk that the glass ampoule in the biological indicator penetrates through a plastic product to tie the hand or the problem that the culture medium in the spore tube flows backwards due to the acting force of other non-special foreign objects is avoided by pinching with hand, the crushing efficiency is high, and the smooth contact of the culture medium in the ampoule and the bacillus on the paper sheet is facilitated.

Furthermore, the mounting panel is the rectangular plate, and two sides that the spout was kept away from to the rectangular plate are connected with the round bar, and the round bar is parallel with stripper plate slip direction.

Furthermore, one end of the extrusion plate, which is far away from the handle, is provided with a circular through hole, and the diameters of the circular through hole and the crushing hole are not smaller than the diameter of the biological indicator pipe body.

Furthermore, two stripper plates stack in the spout, the spout inner wall is equipped with several spacing grooves, is equipped with the slider that several and spacing groove match on the stripper plate.

Furthermore, the two extrusion plates are synchronously moved, when the extrusion plates slide until the circular through hole is superposed with the crushing hole or the circular through hole is arranged in the center of the crushing hole, the circular through holes of the two extrusion plates are superposed, the extrusion plates are continuously pressed, the circular through holes of the two extrusion plates are gradually intersected, and a gap formed by the two extrusion plates is smaller than the diameter of the biological indicator ampoule.

According to the invention, the circular through holes are formed in the mounting plates, when the biological indicator needs to be crushed, the circular through holes in the two mounting plates can be overlapped, then the biological indicator pipe body penetrates through the circular through holes and the crushing holes, then the two extrusion plates are simultaneously extruded, the circular through holes are gradually intersected and separated, and the ampoule is crushed under the extrusion of the two circular through holes, so that the biological indicator crushing effect is achieved.

Further, the knapper connecting piece is for fixing the bar pole in cultivation box one side, is equipped with the cross-section on the bar pole and is convex bar groove, and the bar groove runs through the bar pole, and bar groove diameter matches with the round bar, the knapper passes through the round bar with the cultivation box and the bar groove links to each other, and the round bar can freely overturn at the bar inslot.

A use method of a biological indicator assisted disruption culture device comprises the following steps:

1) taking out the biological indicator tube body after sterilization, and crushing an ampoule of the biological indicator tube body by using a crusher to enable the culture medium in the ampoule to flow out and contact with the bacillus on the paper sheet;

2) vertically placing the biological indicator tube body in a mounting rack culture cavity on a culture box body;

3) and (3) feeding the culture box body into a special culture chamber for constant-temperature culture, taking out after the culture is finished, observing the color change in the biological indicator tube body through a side plate of the culture box body, and judging the sterilization effect according to the color change.

In the step 1), the method for crushing the biological indicator tube ampoule comprises the following steps: the upper end of the biological indicator tube body is arranged in a breaking hole of the breaker mounting plate, the handle is held by a hand, the extrusion plate is pushed inwards, the biological indicator tube body in the breaking hole is extruded by the extrusion plate, an ampoule in the biological indicator tube body is crushed, and the internal culture medium flows out and contacts with the bacillus on the paper sheet.

Compared with the prior art, the invention has the following advantages and beneficial effects:

1. according to the auxiliary crushing culture device for the biological indicator, the biological indicator storage unit in the mounting frame is used for placing the biological indicator, so that the biological indicator can keep a vertical state in the culture process, and biological safety hazards caused by reverse leakage of a spore tube are avoided;

2. according to the auxiliary crushing culture device for the biological indicator, when the ampoule of the biological indicator is crushed, the tube body of the biological indicator is placed in the crushing hole of the mounting plate, the handle is held by hands, the extrusion plate is pushed inwards, the extrusion plate extrudes the tube body of the biological indicator in the crushing hole and crushes the ampoule in the crushing hole, the biological indicator is not needed to be received by hands in the whole process, the risk that the glass ampoule in the biological indicator penetrates through a plastic product to be pricked by hand through pinching by hand or the problem that culture medium in a spore tube flows backwards due to the acting force of other non-special foreign objects is avoided, the crushing efficiency is high, and the smooth contact of the culture medium in the ampoule and bacillus on a piece of paper is facilitated;

3. according to the auxiliary crushing culture device for the biological indicator, the circular through holes are formed in the mounting plates, when the biological indicator needs to be crushed, the circular through holes in the two mounting plates can be overlapped, then the biological indicator pipe body penetrates through the circular through holes and the crushing holes, then the two extrusion plates are simultaneously extruded, the circular through holes are gradually intersected and separated, the ampoule is crushed under the extrusion of the two circular through holes, the crushing effect of the biological indicator is achieved, and the two extrusion plates are simultaneously adopted to act on the ampoule, so that the crushing efficiency is higher.

Drawings

The accompanying drawings, which are included to provide a further understanding of the embodiments of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the principles of the invention. In the drawings:

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of a disruptor of the present invention;

FIG. 3 is a longitudinal section of the fragmenter of the present invention;

FIG. 4 is a cross-sectional view of the fragmenter of the present invention;

FIG. 5 is an intersection of two circular through holes of the breaker of the present invention;

fig. 6 is a longitudinal sectional view of the breaker of the present invention in the state of fig. 5.

Reference numbers and corresponding part names in the drawings:

1-culture box body, 11-side plate, 2-mounting frame, 21-frame, 22-partition plate, 23-culture cavity, 24-short shaft, 3-knapper, 4-knapper connecting piece, 41-strip groove, 5-mounting plate, 51-sliding groove, 52-breaking hole, 53-round rod, 54-limiting groove, 6-extrusion plate, 61-handle, 62-round through hole, 63-sliding block and 7-biological indicator tube body.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples and accompanying drawings, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not meant to limit the present invention.