阅读说明：本技术 一种富集油脂中n-3多不饱和脂肪酸甘油酯的方法 (Method for enriching n-3 polyunsaturated fatty acid glyceride in grease ) 是由 王小三 杨壮壮 程昕祎 高亮 邹硕 金青哲 王兴国 于 2019-10-14 设计创作，主要内容包括：本发明公开了一种富集油脂中n-3多不饱和脂肪酸甘油酯的方法,包括,取油脂、脂肪酶和缓冲溶液于反应器中,反应一定的时间后,得混合物,将混合物通过碱液中和脱酸,得到富含n-3多不饱和脂肪酸的甘油酯。本发明提供一种富集油脂中n-3多不饱和脂肪酸甘油酯的方法,脂肪酶催化水解法直接将鱼油作为原料,反应过程中不添加其他有机溶剂,反应原料为鱼油和缓冲溶液,催化剂为脂肪酶,与传统化学法相比,反应条件温和、副产物少、环保；与酶法酯交换和酯化相比,更加安全和成本更低,工艺更加简单,方法成熟,更适于工业化生产。(The invention discloses a method for enriching n-3 polyunsaturated fatty acid glyceride in grease, which comprises the steps of putting the grease, lipase and buffer solution into a reactor, reacting for a certain time to obtain a mixture, and neutralizing and deacidifying the mixture by alkali liquor to obtain the glyceride rich in n-3 polyunsaturated fatty acid. The invention provides a method for enriching n-3 polyunsaturated fatty acid glyceride in grease, a lipase catalytic hydrolysis method directly takes fish oil as a raw material, no other organic solvent is added in the reaction process, the reaction raw materials are the fish oil and a buffer solution, and the catalyst is lipase; compared with the enzymatic transesterification and esterification, the method is safer, has lower cost, simpler process and mature method, and is more suitable for industrial production.)

1. A method for enriching n-3 polyunsaturated fatty acid glyceride in grease is characterized in that: comprises the steps of (a) preparing a mixture of a plurality of raw materials,

taking oil, lipase and buffer solution to react in a reactor for a certain time to obtain a mixture, and neutralizing and deacidifying the mixture by alkali liquor to obtain the glyceride rich in n-3 polyunsaturated fatty acid.

2. The method for enriching n-3 polyunsaturated fatty acid glycerides in oils and fats according to claim 1, wherein: the oil comprises fish oil.

3. The method for enriching n-3 polyunsaturated fatty acid glycerides in oils and fats according to claim 1, wherein: the lipase is derived from one or more of Candida cylindracea and Rhizopus oryzae.

4. The method for enriching n-3 polyunsaturated fatty acid glycerides in oils and fats according to claim 1, wherein: the lipase derived from candida cylindracea is one or more of AY 'Amano' 400SD and AY 'Amano' 30SD, and the lipase derived from rhizopus oryzae is DF 'Amano' 15.

5. The method for enriching n-3 polyunsaturated fatty acid glycerides in oils and fats according to claim 1, wherein: the buffer solution is one of phosphoric acid buffer solution and citric acid buffer solution, wherein the concentration of the buffer solution is 0.05-0.5 mol/L, and the pH value range of the buffer solution is 5-8.

6. The method for enriching n-3 polyunsaturated fatty acid glycerides in oils and fats according to claim 1, wherein: the mass ratio of the buffer solution to the grease is 0.2-3: 1.

7. The method for enriching n-3 polyunsaturated fatty acid glycerides in oils and fats according to claim 1, wherein: the reaction time is 4-48 h, and the reaction temperature is 10-60 ℃.

8. The method for enriching n-3 polyunsaturated fatty acid glycerides in oils and fats according to claim 1, wherein: the addition amount of the lipase accounts for 0.04-1% of the mass percentage of the grease.

9. The method for enriching n-3 polyunsaturated fatty acid glycerides in oils and fats according to claim 1, wherein: and the alkali liquor neutralization deacidification comprises the steps of transferring the mixture to a separating funnel, adding n-hexane to separate the fish oil from water, adding a phenolphthalein indicator, titrating by using a KOH-ethanol aqueous solution until the system turns red, standing and layering, removing the water phase at the lower layer, distilling and washing, taking the upper clear oil phase, and evaporating the solvent to obtain the n-3 polyunsaturated fatty acid glyceride.

10. The method for enriching n-3 polyunsaturated fatty acid glycerides in fats and oils according to claim 1 or 9, wherein: the alkali liquor is KOH-ethanol aqueous solution or NaOH-ethanol aqueous solution.