阅读说明：本技术 一种利用表面等离子体热效应分区加热的生物芯片 (Biological chip for zone heating by surface plasma heat effect ) 是由 张海汐 何浩培 于 2019-09-25 设计创作，主要内容包括：本发明公开了一种利用表面等离子体热效应分区加热的生物芯片,包含透明基体,所述透明基体的任意一侧设有纳米金属岛膜,纳米金属岛膜在微观上形貌为岛屿状分布,岛的尺度在微米以下,岛之间间距在微米以下,纳米金属岛膜上表面覆盖或者镀有一层用于提供化学保护和防止荧光淬灭的包覆层,包覆层的上方设有生物样本层,本发明即基于表面等离子体热效应分区加热的生物芯片利用了可以强吸收光能制热的纳米金属岛膜作为材料,在具有微纳尺度大小光斑的光源照射下,实现了发热区域小、热源局域性高、非接触式制热等特性。(The invention discloses a biochip for heating in a partition mode by utilizing a surface plasma thermal effect, which comprises a transparent substrate, wherein a nano metal island film is arranged on any one side of the transparent substrate, the nano metal island film is microscopically distributed in an island shape, the size of islands is below micrometers, the interval between islands is below micrometers, a coating layer for providing chemical protection and preventing fluorescence quenching is covered or plated on the upper surface of the nano metal island film, and a biological sample layer is arranged above the coating layer.)

1. The biochip for zone heating by utilizing the thermal effect of the surface plasma comprises a transparent substrate (1), and is characterized in that a nano metal island film is arranged on any one side of the transparent substrate (1), the shape of the nano metal island film is microscopically distributed in an island shape, the size of each island is below micrometers, the distance between the islands is below micrometers, a coating layer (2) used for providing chemical protection and preventing fluorescence quenching is covered or plated on the upper surface of the nano metal island film, and a biological sample layer (3) is arranged above the coating layer (2).

2. The biochip for heating by zoning based on thermal effect of surface plasma as claimed in claim 1, wherein the nano metal island film is made of nano gold/silver by quenching.

3. A surface plasmon heating effect zone heating biochip according to claim 1, characterized in that the coating layer (2) is one of a silicon dioxide layer, organic glass pmma layer or pdms layer.

4. A biochip for heating by zones with thermal effect of surface plasmons according to claim 3, characterized in that the thickness of the coating (2) is between 10nm and 50 nm.

5. A biochip for zonal heating by the thermal effect of surface plasmons according to claim 1, wherein the transparent substrate (1) is glass.

Technical Field

The invention relates to the technical field of biochips, in particular to a biochip for heating in a subarea mode by utilizing the thermal effect of surface plasmas.

Background

Biochips, also called protein chips or gene chips, originate from crystallization combining DNA hybridization probe technology with semiconductor industry technology. The technology is that a large number of probe molecules are fixed on a support and then hybridized with DNA with fluorescent labels or other sample molecules (such as proteins, factors or small molecules), and the quantity and sequence information of the sample molecules are obtained by detecting the hybridization signal intensity of each probe molecule.

The existing biochip aims to integrate biochemical reaction in an area as small as possible, but simultaneously, separate temperature control is often required to be carried out on different partitioned cavities, and inevitable contradiction exists between pursuing high integration level and partitioned heating. Heating by electronic means may be subject to the size limitations of electronic temperature control elements and may not further improve integration.

Summary of the invention

The present invention aims to provide a biochip for heating by surface plasma thermal effect in a partitioned manner, so as to solve the problems in the background art.

In order to achieve the above purpose, the invention provides the following technical scheme:

the utility model provides an utilize biological chip of surface plasma heat effect subregion heating, contains transparent base member, arbitrary one side of transparent base member is equipped with nanometer metal island membrane, and nanometer metal island membrane is distribution for island form in the microcosmic appearance, and the yardstick of island is below the micron, and the interval is below the micron between the island, and nanometer metal island membrane upper surface covers or plates one deck and is used for providing chemical protection and prevent the coating of fluorescence quenching, and the top of coating is equipped with biological sample layer.

The invention adopts the following further technical scheme: the nano metal island film is made of nano gold/silver through quenching.

The invention adopts the following further technical scheme: the coating layer is one of a silicon dioxide layer, an organic glass pmma layer or a pdms layer.

The invention adopts the following further technical scheme: the thickness of the coating layer is 10nm to 50 nm.

The invention adopts the following further technical scheme: the transparent substrate is glass.

Compared with the prior art, the invention has the beneficial effects that: the invention is a biochip based on surface plasma heat effect zone heating, which utilizes a nano metal island film capable of strongly absorbing light energy to heat as a material, and realizes the characteristics of small heating area, high heat source locality, non-contact heating and the like under the irradiation of a light source with light spots of micro-nano scale.

Drawings

Fig. 1 is an overall structural view of the present invention.

FIG. 2 is a schematic diagram of island subsections of a nano-metal island film.

In the figure: 1-transparent matrix, 2-coating layer and 3-biological sample layer.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments to be described herein without making any creative effort, shall fall within the protection scope of the present invention.