阅读说明：本技术 一种烟草种子的活力测定方法 (A kind of vigour-testing method of tobacco seed ) 是由 马文广 潘威 杨晓东 宋碧清 乔雨 杜景诚 于 2019-09-29 设计创作，主要内容包括：本发明公开了一种烟草种子的活力测定方法,所述的烟草种子的活力测定方法包括置种、低温吸涨、标准发芽、正常幼苗统计和结果记录步骤。本发明采用置种、低温吸涨、标准发芽、正常幼苗统计的步骤进行烟草种子活力测定,相对于标准发芽理想条件,该先法首先利用我国烟草种子漂浮育苗过程中,最常遭遇的低温、快速吸涨逆境处理种子,再进行标准发芽,其活力结果比标准发芽条件下发芽率与出苗率相关性更高,更能代表种子田间实际出苗情况,可指导烟草种子生产单位选择高活力种子进行加工销售,提高产品质量,降低种子在烟区育苗过程遭遇逆境后出苗率不达标风险,同时降低烟草育苗单位的管理、间苗成本等。该法标准发芽时间156 h~180 h,相对于标准发芽规定的14 d,大大节省了检测时间。总之,该法详细规定了成本低廉,操作简单,效果显著,已具实际规模应用可能。(The invention discloses a kind of vigour-testing method of tobacco seed, the vigour-testing method of the tobacco seed include set kind, low temperature imbibition, standard germination, normal seedling statistics and result recording step.The present invention uses and sets kind, low temperature imbibition, standard germination, the step of normal seedling counts carries out Tobacco seed vitality measurement, relative to standard germination ideal conditions, during elder generation's method is first with China's tobacco seed floating seedlings, the low temperature most often met with, quick imbibition adverse circumstance handles seed, standard germination is carried out again, its vigor result is higher than germination percentage under the conditions of standard germination and emergence rate dependence, more representative of the practical emergence in seed field, it can instruct tobacco seed production unit that high vigor seed is selected to carry out processing sell, improve product quality, reduce seed emergence rate risk not up to standard after cigarette district seedling raising process meets with adverse circumstance, the management of tobacco seedling unit is reduced simultaneously, thinning cost etc..Detection time is greatly saved relative to 14 d as defined in standard germination in 156 h of h ~ 180 of the method standard germination time.In short, low in cost, easy to operate, the significant effect of the method specified in more detail, having had practical sizable application may.)

1. a kind of vigour-testing method of tobacco seed, it is characterised in that the vigour-testing method of the tobacco seed includes setting Kind, low temperature imbibition, standard germination, normal seedling statistics and result recording step, specifically include:

A, it sets kind: taking and set kind of a container, set reservoir bed setting kind of a container bottom pad, pad sets water absorption layer, reservoir bed storage above reservoir bed Full water to water suction layer surface is moisture state, then uniformly places tobacco seed to be measured in water suction layer surface；

B, low temperature imbibition: kind of the container of setting for placing tobacco seed to be measured is placed under 3 ~ 8 DEG C of environment of temperature and allows tobacco seed low temperature Imbibition 90h ~ 100h；

C, kind of a container of setting for the placement tobacco seed to be measured that step B obtains standard germination: is placed in 20 DEG C ~ 30 DEG C conditions of temperature Under, by daily illumination 12h, dark 12h to Tobacco Seed Germination, during which keeping water suction layer surface is moisture state；

D, normal seedling counts: 156h ~ 180h records germination quantity after tobacco seed standard germination, carries out germination system Meter；

E, result records: Tobacco seed vitality is recorded according to following equation, counts 3 ~ 4 repetition average values, formula is as follows:

。

2. the vigour-testing method of tobacco seed according to claim 1, it is characterised in that described sets kind of container as training Support ware.

3. the vigour-testing method of tobacco seed according to claim 1, it is characterised in that the reservoir bed is sponge.

4. the vigour-testing method of tobacco seed according to claim 1, it is characterised in that the water absorption layer is filter paper.

5. the vigour-testing method of tobacco seed according to claim 1, it is characterised in that the kind of setting is to take 100 Mm culture dish, pad sets one layer of sponge in culture dish, then pads two layers of filter paper, it is complete to filter paper that 20 ml tap water are added in sponge Until portion is wet, 100 tobacco seeds, cross 10 × perpendicular 10 are uniformly placed on filter paper, kind grain is spaced 5 mm or more anyhow, raises Filter paper but guarantee that seed position is constant, is added on 10 ml tap water to sponge again, covers culture dish lid, each detection batch 3 ~ 4 repetitions are arranged in number seed.

6. the vigour-testing method of tobacco seed according to claim 1, it is characterised in that when germination statistics, seedling radicle Normally, can normal growth elongation, there are 2 green cotyledons, cotyledon shape is complete and is ellipse, and seedling entirety size is unobvious It is weaker than seedling around and is calculated as normal chitting piece, and has no prominent kind of the skin of radicle, radicle atrophy, rarely seen radicle and have no cotyledon, 2 Cotyledon does not completely disengage that kind of a skin, cotyledon yellow, unifacial leaf, 3 cotyledons, cotyledon deformity, seedling is extremely weak etc. is counted as abnormal kind Son.

Technical field

The invention belongs to technical field of tobacco, and in particular to a kind of vigour-testing method of tobacco seed.

Background technique

Enter high quality developing new stage with tobacco business, promotes tobacco product quality using scientific and technical innovation, it is more preferable full Sufficient customer demand becomes the road of certainty.Tobacco seed improves its quality as the means of production most basic in leaf tobacco production It is that tobacco seed company struggles away to develop forefront important means in high quality with scientific and technological content.Currently, tobacco seed quality is main It is detected by the germination percentage that standard germination is tested, and germination percentage only represents seed whether has viability, is under ecotopia It is no to sprout, it not can accurately reflect seed actually emergence performance in complicated field conditions.And seed vitality is seed science The New Set for the measurement seed quality that worker puts forward, international Seed Inspection association (ISTA) also make seed vitality concept Regulation, refers to the overall characteristic of different aspect when the seed lot that can measure high-incidence bud rate is emerged under extensive environment, can be accurate Reflect seed field practical manifestation, can further promote tobacco seed quality using it as examination criteria.Seed vitality is to measure kind The important indicator of protonatomic mass, high vigor seed have apparent growth vigor and productive potentialities, have to agricultural production very heavy The meaning wanted.Though it is had been reported that both at home and abroad about Tobacco seed vitality detection method, it is mostly time-consuming, expensive, laborious, it is actually suitable for The method of enterprise practical application does not have but.

Summary of the invention

The purpose of the present invention is to provide a kind of vigour-testing methods of tobacco seed.

The object of the present invention is achieved like this, the vigour-testing method of the tobacco seed include set kind, low temperature is inhaled Rise, standard germination, normal seedling statistics and result recording step, specifically include:

A, it sets kind: taking and set kind of a container, set reservoir bed setting kind of a container bottom pad, pad sets water absorption layer, reservoir bed storage above reservoir bed Full water to water suction layer surface is moisture state, then uniformly places tobacco seed to be measured in water suction layer surface；

B, low temperature imbibition: kind of the container of setting for placing tobacco seed to be measured is placed under 3 ~ 8 DEG C of environment of temperature and allows tobacco seed low temperature Imbibition 90h ~ 100h；

C, standard germination: by setting under the conditions of kind of a container is placed in 20 ~ 30 DEG C of temperature for placement tobacco seed to be measured that step B obtains, By daily illumination 12h, dark 12h to Tobacco Seed Germination, during which keeping water suction layer surface is moisture state；

D, normal seedling counts: 156h ~ 180h records germination quantity after tobacco seed standard germination, carries out germination system Meter；

E, result records: Tobacco seed vitality is recorded according to following equation, counts 3 ~ 4 repetition average values, formula is as follows:

。

The specific operation method is as follows for the vigour-testing method of tobacco seed of the present invention:

(1) it sets kind: taking 100 mm culture dishes, pad sets one layer of sponge in culture dish, then pads two layers of filter paper, is added 20 in sponge Ml tap water uniformly places 100 tobacco seeds, cross 10 × perpendicular 10, kind grain is vertical and horizontal until filter paper is all wet on filter paper 5 mm or more are spaced, filter paper is raised but guarantee that seed position is constant, is added on 10 ml tap water to sponge again, covers culture 3 ~ 4 repetitions are arranged in ware lid, each detection lot number seed；

(2) low temperature imbibition:；The culture dish that tobacco seed is housed in (1) is placed as in growth cabinet, incubator temperature is adjusted It is 3 DEG C ~ 8 DEG C, allows the tobacco seed low temperature imbibition h of 90h ~ 100, keep incubator temperature-resistant during imbibition, and moisture is sufficient；

(3) standard germination: culture dish after imbibition is taken in (2), is placed in 25 DEG C of incubators, daily 12 h of illumination, 12 h of dark, light Keep filter paper wet according to 1000 lx or more of intensity, during germination, within the temperature difference ± 2 DEG C；

(4) normal seedling counts: the h of 156 h ~ 180 records germination quantity after seed standard germination, counts normal germination Seed amount.When germination statistics, seedling radicle is normal, can normal growth elongation, there are 2 green cotyledons, cotyledon shape is complete And be ellipse, seedling entirety size is unobvious to be weaker than seedling around and is calculated as normal chitting piece, and have no prominent kind of the skin of radicle, Radicle atrophy, rarely seen radicle have no that cotyledon, 2 cotyledon do not completely disengage kind of a skin, cotyledon yellow, unifacial leaf, 3 cotyledons, cotyledon Deformity, seedling is extremely weak etc. is counted as abnormal seed；

(5) result records: Tobacco seed vitality is recorded according to following equation, counts 3 ~ 4 repetition average values, formula is as follows:

。

The present invention is directed to existing tobacco seed quality determining method --- and what standard germination detected can not Accurate Prediction seed-breeding field Between emerge result the shortcomings that, provide more can accurately reflect tobacco seed quality and can practical operation application vigor testing methods, Further promote tobacco seed quality.

The present invention using set kind, low temperature imbibition, standard germination, normal seedling statistics the step of carry out Tobacco seed vitality survey It is fixed, it is most often met with during elder generation's method is first with China's tobacco seed floating seedlings relative to standard germination ideal conditions Low temperature, quick imbibition adverse circumstance handle seed, then carry out standard germination, and vigor result is than germination percentage under the conditions of standard germination and goes out Seedling rate dependence is higher, more representative of the practical emergence in seed field, tobacco seed production unit can be instructed to select high vigor Seed carries out processing sell, improves product quality, reduces seed emergence rate wind not up to standard after cigarette district seedling raising process meets with adverse circumstance Danger, while reducing the management of tobacco seedling unit, thinning cost etc..In addition, the method sets kind of process before adverse circumstance processing, seed is not Absorb water moisture content, and vacuum can be used and set kind of instrument or the progress scale sowing of other seeding apparatus, improve sowing efficiency, be conducive to the method Scale application；The adverse environmental factor is that China's cigarette district most often meets with adverse circumstance, and result necessarily has higher generation table, and the adverse circumstance Condition is easily realized in laboratory.156 h of h ~ 180 of the method standard germination time, relative to 14 d as defined in standard germination, Detection time is greatly saved.In short, the method specified in more detail is low in cost, easy to operate, significant effect had practical scale Using possible.

Detailed description of the invention

Fig. 1 is to utilize 97 seed 7d germination performance schematic diagrames of lot number cloud and mist different under the conditions of standard germination；

Fig. 2 is to show schematic diagram using 97 seed vitality of the method for the present invention difference lot number cloud and mist.

Specific embodiment

Below with reference to embodiment and attached drawing, the present invention is further illustrated, but is not subject in any way to the present invention Limitation, based on present invention teach that it is made it is any transform or replace, all belong to the scope of protection of the present invention.

The vigour-testing method of tobacco seed of the present invention include set kind, low temperature imbibition, standard germination, normal seedling Statistics and result recording step, specifically include:

A, it sets kind: taking and set kind of a container, set reservoir bed setting kind of a container bottom pad, pad sets water absorption layer, reservoir bed storage above reservoir bed Full water to water suction layer surface is moisture state, then uniformly places tobacco seed to be measured in water suction layer surface；

B, low temperature imbibition: kind of the container of setting for placing tobacco seed to be measured is placed under 3 DEG C ~ 8 DEG C environment of temperature and makes tobacco seed low Warm imbibition 90h ~ 100h；

C, kind of a container of setting for the placement tobacco seed to be measured that step B obtains standard germination: is placed in 20 DEG C ~ 30 DEG C conditions of temperature Under, by daily illumination 12h, dark 12h to Tobacco Seed Germination, during which keeping water suction layer surface is moisture state；

D, normal seedling counts: 156h ~ 180h records germination quantity after tobacco seed standard germination, carries out germination system Meter；

E, result records: Tobacco seed vitality is recorded according to following equation, counts 3 ~ 4 repetition average values, formula is as follows:

。

Kind of the container of setting is culture dish.

The reservoir bed is sponge.

The water absorption layer is filter paper.

The kind of setting is to take 100 mm culture dishes, and pad sets one layer of sponge in culture dish, then pads two layers of filter paper, sponge 20 ml tap water of middle addition uniformly place 100 tobacco seeds until filter paper is all wet on filter paper, cross 10 × perpendicular 10, Kind grain is spaced 5 mm or more anyhow, raises filter paper but guarantees that seed position is constant, is added on 10 ml tap water to sponge again, Culture dish lid is covered, 3 ~ 4 repetitions are arranged in each detection lot number seed.

When germination statistics, seedling radicle is normal, can normal growth elongation, there are 2 green cotyledons, cotyledon shape it is complete and For ellipse, seedling entirety size is unobvious to be weaker than seedling around and is calculated as normal chitting piece, and has no radicle prominent kind of skin, embryo It is abnormal that root atrophy, rarely seen radicle have no that cotyledon, 2 cotyledon do not completely disengage kind of a skin, cotyledon yellow, unifacial leaf, 3 cotyledons, cotyledon Shape, seedling are extremely weak etc. to be counted as abnormal seed.

With specific embodiment, the present invention will be further described below, these embodiments are of the invention and unlimited for illustrating In limiting the scope of the invention.Implementation condition used in the examples can condition according to specific needs do further adjustment, The implementation condition being not specified is usually the condition in routine experiment.