阅读说明：本技术 黄粉虫蛋白质提取多肽胺基酸的方法以及氨基酸水溶肥 (The method and amino acid Water soluble fertilizer of tenebrio molitor protein extraction polypeptide Amino acid ) 是由 朱湘林 许建初 萨迪亚·纳迪尔 塞隆·汗 于 2019-09-23 设计创作，主要内容包括：本发明提供一种黄粉虫蛋白质提取多肽胺基酸的方法以及氨基酸水溶肥,将黄粉虫幼虫干品粉碎成粉末、高温灭菌得到黄粉虫粉,与纯净水混合,虫粉质量占30％；放入生物反应器,加入碱性蛋白酶,水解反应10小时,再将温度升高至80℃,灭酶待温度降50℃时出罐；将水解的混合液泵进脱脂分离器中,在冷却过程中产生脂液离层,将脱脂分离器下部的开关打开让液体流出,进入离心机分离,即获得氨基酸水解液。本发明通过对蛋白酶的不用脱脂直接水解,在工艺上进行改进,将脱脂环节改在水解之后,这样就能大幅减少成本,使工艺简化。在此基础上再将硼酸和硫酸锌熬合到该水解氨基酸中,得到高档水溶肥。(The present invention provides the method and amino acid Water soluble fertilizer that a kind of tenebrio molitor protein extracts polypeptide Amino acid, Yellow meal worm larva dry product is ground into powder, high-temperature sterilization obtains yellow meal worm powder, mixes with pure water, and worm silty amount accounts for 30%；It is put into bioreactor, is added alkali protease, hydrolysis 10 hours, then raise the temperature to 80 DEG C, enzyme deactivation tank out when temperature drops 50 DEG C；By the mixture pump of hydrolysis into degreasing separator, rouge liquid absciss layer is generated in cooling procedure, is allowed liquid to flow out the switch opening of degreasing separator lower part, is separated into centrifuge, i.e. acquisition amino acid hydrolyticsolution.The present invention improves in technique by not having to degreasing direct hydrolysis to protease, degreasing link is changed after hydrolysis, cost can thus be greatly decreased, simplify technique.Boric acid and zinc sulfate are boiled together into the hydrolysis amino acid again on this basis, obtain high-grade Water soluble fertilizer.)

1. the method for tenebrio molitor protein extraction polypeptide Amino acid, which comprises the following steps:

(1) Yellow meal worm larva dry product is ground into 100 mesh powder, high-temperature sterilization 20 minutes, obtains yellow meal worm powder；

(2) yellow meal worm powder is mixed with pure water, worm silty amount accounts for 30%, and water quality accounts for 70%；

(3) mixing liquid is put into bioreactor, parameter is set, PH:7, temperature: 55 DEG C, agitation revolution: 200rpm, oxygen-containing: 20%；

(4) alkali protease is added into bioreactor, additional amount is the 0.5% of yellow meal worm dry product weight；

(5) hydrolysis 10 hours, then raise the temperature to 80 DEG C, reaction half an hour enzyme deactivation tank out when temperature drops 50 DEG C；

(6) mixture pump of hydrolysis is generated rouge liquid absciss layer in cooling procedure, then separates degreasing into degreasing separator The switch opening of device lower part allows liquid to flow out, and lacca then stays in filter frame, takes out filter frame and separates lacca；

(7) liquid separated from degreasing separator is entered into centrifuge separation, i.e. acquisition amino acid hydrolyticsolution.

2. the method that tenebrio molitor protein according to claim 1 extracts polypeptide Amino acid, which is characterized in that described is de- Rouge separator, including taper cone barrel, the taper cone barrel upper half are cylinder, and lower half is conical bottom, are placed in the cylinder There is cartridge filter, filter screen is cased in the cartridge filter, the conical bottom is connected with outlet tube, and the outlet tube is equipped with Switch.

3. amino acid Water soluble fertilizer, which is characterized in that boiled together institute by claim 1 gained amino acid hydrolyticsolution and boric acid and zinc sulfate .

Technical field

The invention belongs to bio-fertilizer technical fields, and in particular to a kind of method that tenebrio molitor protein extracts polypeptide Amino acid And amino acid Water soluble fertilizer.

Background technique

Yellow meal worm is insect ridge, coleoptera, meal beetle Eimeria, and larva dry product protein content is up to 40%, fat 30%, Larva dry product is the optimum feed stock of hydrolysis amino acid, is first to slough insect fat, then the elder brother that will be left behind according to existing technology Worm albumen is hydrolyzed, and the main purpose done so is to drop bottom worm powder and the ratio (ratio of water to material) of water to make if ratio of water to material is excessively high It is feeding-up at mixed liquor in reactor, coming into full contact with for protease and protein substrate is influenced, hydrolysis effect is influenced, meanwhile, hydrolyzate It reduces.Prior art is more complicated and at high cost.

The number of applying for a patent: CN201110299242.1, this method have the following problems, 1, yellow meal worm degreasing is in enzyme digestion reaction Before, that is, yellow meal worm want first degreasing it is complete after obtain degreasing worm powder digested, the technique of this insect degreasing is exactly that Asia is faced Boundary is actually generating, and the equipment of subcritical degreasing is more complicated, and there are also matched boilers in addition to degreasing equipment, accounts for place Area is big, and energy consumption is high, maintenance usually, and maintenance has cost, and in-house facility is also expensive；2, enzyme and tryptose used in the program Enzyme, the protein hydrolysate concentration of substrate ratio of water to material of the protease are 1:3 (degreasing worm powder 1, water 3) if calculated to the dry worm powder of non-degreasing Ratio should be 1:1, and such insect dosage is with regard to big.

In addition, the scheme of the prior art is yellow meal worm, preceding degreasing is hydrolyzed, degreasing equipment used in this method is subcritical It is sudden to take technology, steam is needed due to subcritical, so corollary equipment is there are also an electric heating steam boiler, power consumption is big, equipment land occupation Area is also big, this kind of equipment usually maintenance expenditure be also it is bigger,

Summary of the invention

In view of the above technical problems, the present invention provides a kind of method that tenebrio molitor protein extracts polypeptide Amino acid, selects Alkali protease yellow meal worm does not have to degreasing direct hydrolysis, the hydrolyzate of amino acid content >=10% can be equally obtained, after hydrolysis Mixing liquid can generate water rouge absciss layer while cooling, and lacca is separated, and avoid and use brought by subcritical degreasing not Just and Gao Chengben, worm amount is reduced.

The method of tenebrio molitor protein extraction polypeptide Amino acid, comprising the following steps:

(1) Yellow meal worm larva dry product is ground into 100 mesh powder, high-temperature sterilization 20 minutes, obtains yellow meal worm powder；

(2) yellow meal worm powder is mixed with pure water, worm silty amount accounts for 30%, and water quality accounts for 70%；

(3) mixing liquid is put into bioreactor, parameter is set, PH:7, temperature: 55 DEG C, agitation revolution: 200rpm, It is oxygen-containing: 20%；

(4) alkali protease is added into bioreactor, additional amount is the 0.5% of yellow meal worm dry product weight；

(5) hydrolysis 10 hours, then 80 DEG C are raised the temperature to, reaction half an hour enzyme deactivation goes out when temperature drops 50 DEG C Tank；

(6) mixture pump of hydrolysis is generated into rouge liquid absciss layer, then by degreasing into degreasing separator in cooling procedure The switch opening of separator lower part allows liquid to flow out, and lacca then stays in filter frame, takes out filter frame and separates lacca；

(7) liquid separated from degreasing separator is entered into centrifuge separation, i.e. acquisition amino acid hydrolyticsolution.

The degreasing separator, including taper cone barrel, the taper cone barrel upper half are cylinder, and lower half is conical bottom, It is placed with cartridge filter in the cylinder, filter screen is cased in the cartridge filter, the conical bottom is connected with outlet tube, institute The outlet tube stated is equipped with switch.

The present invention also provides a kind of amino acid Water soluble fertilizer, above-mentioned gained amino acid hydrolyticsolution and boric acid and zinc sulfate boil together institute .

The present invention by protease to filter out a kind of pair of yellow meal worm albumen identity more preferable, determine that alkali protease is done For hydrolase, due to the specificity of enzyme, alkali protease only to protein have a hydrolysis and to fatty Fails To Respond, thus it is yellow Mealworm powder can be hydrolyzed with rouge.It without degreasing direct hydrolysis, is improved in technique, degreasing link is changed after hydrolysis, Cost can thus be greatly decreased, technique is made to simplify the index for being also easy to operate while can also reach design.

The present invention is that Yellow meal worm larva dry powder is hydrolyzed by protease, and the protein in yellow meal worm is hydrolyzed At polypeptide amino acid hydrolyzate, the amino acid content in the liquid, which will reach state specified standards i.e. 18 kind total amino acid content, to be wanted >=10%, boric acid and zinc sulfate are boiled together into the hydrolysis amino acid again on this basis, the total amount of zinc and boron will reach 2%, Make a high-grade Water soluble fertilizer agriculturally used.

Detailed description of the invention

Fig. 1 is the structural schematic diagram of degreasing separator of the invention.

Specific embodiment

The subject matter of technical solution of the present invention:

The method of tenebrio molitor protein extraction polypeptide Amino acid, comprising the following steps:

(1) Yellow meal worm larva dry product is ground into 100 mesh powder, high-temperature sterilization 20 minutes, obtains yellow meal worm powder；

(2) yellow meal worm powder is mixed with pure water, worm silty amount accounts for 30%, and water quality accounts for 70%；

(3) mixing liquid is put into bioreactor, parameter is set, PH:7, temperature: 55 DEG C, agitation revolution: 200rpm, It is oxygen-containing: 20%；

(4) alkali protease is added into bioreactor, additional amount is the 0.5% of yellow meal worm dry product weight；

(5) hydrolysis 10 hours, then 80 DEG C are raised the temperature to, reaction half an hour enzyme deactivation goes out when temperature drops 50 DEG C Tank；

(6) mixture pump of hydrolysis is generated into rouge liquid absciss layer, then by degreasing into degreasing separator in cooling procedure The switch opening of separator lower part allows liquid to flow out, and lacca then stays in filter frame, takes out filter frame and separates lacca；

(7) liquid separated from degreasing separator is entered into centrifuge separation, i.e. acquisition amino acid hydrolyticsolution.

Present invention eliminates subcritical kingfisher taking techniques needed for insect degreasing, and the degreasing work of lacca is placed on hydrolysis and is completed After, the present invention provides a kind of simple degreasing separator of structure, and lacca is separated.

As shown in Figure 1, the degreasing separator, including taper cone barrel 1,1 upper half of taper cone barrel are cylinder, lower half Portion is conical bottom, and cartridge filter 2 is placed in the cylinder, filter screen 3, the conical bottom are cased in the cartridge filter 2 It is connected with outlet tube 4, the outlet tube 4 is equipped with switch.

Taper cone barrel 1 is stainless steel structure, and discharge port and switch are arranged at bottom.

Cartridge filter 2 is two cylinder-shaped stainless steel screen frames for grabbing ear, and the overall diameter of cartridge filter 2 is bore in taper cone barrel 1 Sub-calibre, cartridge filter 2 are wanted to go in.Filter screen 3 is the filter cloth of 150 mesh covered on cartridge filter 2.

Degreasing separator work principle: the mixing liquid hydrolyzed in bioreactor being pumped and is carried out in degreasing separator, Since hydrolyzed insect protein is changed into polypeptide and amino acid, is completely disengaged with insect fat, will appear absciss layer naturally, Fat floats on above, and hydrolysis amino acid sinks to following.Amino acid hydrolyticsolution is opened from 4 gate out switch of outlet tube, hydrolyzes liquid stream Out, it is drawn off removing lacca when more dry to the lacca in stainless steel framework.

Ratio of water to material of the present invention: referring to the ratio of worm powder and water, and the ratio of water to material of selection is 30%, i.e. 30% water of the powder of worm containing rouge 70%, since the fat of Yellow meal worm larva dry product is 30%, albumen is 40%, and protein is 12% in 30% yellow meal worm powder, As long as therefore rationally, the hydrolysis amino acid that 12% protein is hydrolyzed into 10% can obtain for hydrolysis parameters design.

=after protease enters bioreactor, 30% ratio of water to material can make mixed liquor feeding-up, be detrimental in this way What protease and protein substrate came into full contact with, therefore being unfavorable for hydrolysis should be turned up in the preceding half an hour blender revolution of hydrolysis A bit, it should be adjusted to 300rpm, recalling to 200rpm after half an hour again keeps its stirring steady, while oxygen supply 20%.

The present invention extends hydrolysis time, extends to 10 hours by original 6 hours, can obtain more hydrolysis amino in this way Acid.

Technical solution of the present invention bring is effective to be omitted insect degreasing link, simplifies technique and reduces costs, By 1 ton of amino acid hydrolyticsolution of daily output, required subcritical kingfisher takes tank approximately 10 contingency platform, the electric steam boiler of mating 700KW 3.5 ten thousand yuan are wanted, required pipeline and water pump also want 2.5 ten thousand yuan or so in system, press 8 hours production institute power consumption, equipment within one day When system power consumption 350KW/, these are all extra spendings, need 2 people operation, and the present invention does not have to because changing protease, yellow meal worm Degreasing ratio of water to material is concentration 30%, and actually fewer than amount used in prior art, the present invention is than prior art side Case uses yellow meal worm less, and the prior art is 2.8 times or so of worm amount of the invention.