阅读说明：本技术 一种提高红霉素发酵效价的发酵方法 (A kind of fermentation process improving abomacetin fermentation potency ) 是由 于荣 王晓军 王少云 郭佳 梁丽娟 于 2019-09-18 设计创作，主要内容包括：本发明涉及一种提高红霉素发酵效价的发酵方法,该发酵方法是采用以红色链霉菌为发酵菌种、由种子培养和发酵培养构成的二级发酵模式发酵生产,本发明通过改变种子及发酵培养基的配方结构,祛除了黄豆饼粉的使用,补入硝酸钠溶液作为氮源,并引入微量元素钴,改善了发酵过程中的供氧条件,降低了供氧的能源消耗,从而增强菌体活力,延长红霉素分泌期,提高了红霉素发酵水平。(The present invention relates to a kind of fermentation process for improving abomacetin fermentation potency, the fermentation process is to use using streptomyces erythreus as fermenting microbe, the second order fermentation mode fermenting and producing being made of seed culture and fermented and cultured, the present invention passes through change seed and the formulation composition of fermentation medium, the use of soybean cake powder is dispelled, sodium nitrate solution is filled into as nitrogen source, and introduce trace element cobalt, improve the oxygen supply condition in fermentation process, reduce the energy consumption of oxygen supply, to enhance thallus vigor, extend the erythromycin secretory phase, improves abomacetin fermentation level.)

1. it is a kind of improve abomacetin fermentation potency fermentation process, it is characterised in that use using streptomyces erythreus as fermenting microbe, The second order fermentation mode fermenting and producing being made of seed culture and fermented and cultured,

Wherein seed culture medium used in seed culture forms are as follows: starch 2~4%, bean powder 2~4%, dextrin 1~3%, corn pulp 1 ~3%, ammonium sulfate 0.1~0.3%, precipitated calcium carbonate 0.3~0.8%, magnesium sulfate 0.1~0.3%, cobalt chloride 0.01~0.03%, fit Dimethicone defomaing agent is measured, remaining is water, by weight percentage；

The composition of fermentation medium used in fermented and cultured are as follows: starch 2.0~4.0%, corn pulp 2.0~4.0%, ammonium sulfate 0.1~ 0.4%, calcium carbonate 0.7~1.0%, magnesium sulfate 0.1~0.3%, cobalt chloride 0.02~0.05%, appropriate dimethicone defomaing agent, Remaining is water, with weight by volume basis.

2. the fermentation process described in accordance with the claim 1 for improving abomacetin fermentation potency, it is characterised in that the seed culture Condition are as follows: 34 DEG C~36 DEG C of cultivation temperature, ventilatory capacity 1:08~1.0v/v/min, 45 hr of incubation time~50hr.

3. the fermentation process described in accordance with the claim 1 for improving abomacetin fermentation potency, it is characterised in that the fermented and cultured Condition are as follows: 32 DEG C~36 DEG C of cultivation temperature, dissolved oxygen is greater than 20%, 160~170hr of incubation time.

4. according to the fermentation process for improving abomacetin fermentation potency described in claim 1 or 3, which is characterized in that fermenting Feed supplement is carried out in journey, including is mended sodium nitrate, mended sugar and correction butanol, wherein

It mends sodium nitrate: filling into sodium nitrate solution in 40~150hr of fermented and cultured, control amino nitrogen content 20~30% in fermentation liquid；

Mend sugar: starting to fill into glucose solution when total reducing sugar is down to 1.8% or less in fermentation liquid, control total reducing sugar 1.0 in fermentation liquid~ 2.0%；

Correction propyl alcohol: filling into n-butanol after the 20hr that ferments, and controls normal propyl alcohol content 0 .05~0 .10% in fermentation liquid.

5. improving the fermentation process of abomacetin fermentation potency according to claim 4, it is characterised in that the sodium nitrate is molten Liquid mass-volume concentration be 15~25%, glucose solution mass concentration be 20~30%, normal propyl alcohol concentration of polymer solution be 30~ 40%。

Technical field

The present invention relates to field of microbial fermentation, more particularly to a kind of fermentation process for improving abomacetin fermentation potency.

Background technique

Erythromycin (erythromycin, Er) is a kind of alkaline antibiotic, mainly includes Erythromycin A (ErA), berythromycin (ErB), the components such as Erythromycin C (ErC) and Erythromycin E (ErE), antimicrobial spectrum is approximate with penicillin, and clinic is mainly used in chain Coccigenic tonsillitis, scarlet fever, diphtheria and carrier, stranguria syndrome etc..Recently as the research and development to erythromycin, make The demand of erythromycin continues to increase.

Summary of the invention

The object of the invention is that overcoming the defect of the above-mentioned prior art, one kind is provided and effectively improves the plain energy of erythromycin production The erythrocin fermentation method of power and quality of finished.

The technical solution taken for achieving the above object are as follows:

It is a kind of improve abomacetin fermentation potency fermentation process, it is characterised in that use using streptomyces erythreus as fermenting microbe, by The second order fermentation mode that seed culture and fermented and cultured are constituted,

Wherein seed culture medium used in seed culture forms are as follows: starch 2~4%, bean powder 2~4%, dextrin 1~3%, corn pulp 1 ~3%, ammonium sulfate 0.1~0.3%, precipitated calcium carbonate 0.3~0.8%, magnesium sulfate 0.1~0.3%, cobalt chloride 0.01~0.03%, fit Dimethicone defomaing agent is measured, remaining is water, by weight percentage；

The composition of fermentation medium used in fermented and cultured are as follows: starch 2.0~4.0%, corn pulp 2.0~4.0%, ammonium sulfate 0.1~ 0.4%, calcium carbonate 0.7~1.0%, magnesium sulfate 0.1~0.3%, cobalt chloride 0.02~0.05%, appropriate dimethicone defomaing agent, Remaining is water, with weight by volume basis.

The seed culture condition are as follows: 34~36 DEG C of cultivation temperature, ventilatory capacity 1:08~1.0v/v/min, incubation time 45 ~50hr.

Fermentation culture conditions are as follows: 32~36 DEG C of cultivation temperature, dissolved oxygen is greater than 20%, 160~170hr of incubation time.

Feed supplement is carried out during the fermentation, including is mended sodium nitrate, mended sugar and correction butanol, wherein

It mends sodium nitrate: filling into sodium nitrate solution in 40~150hr of fermented and cultured, control amino nitrogen content 20~30% in fermentation liquid；

Mend sugar: starting to fill into glucose solution when total reducing sugar is down to 1.8% or less in fermentation liquid, control total reducing sugar 1.0 in fermentation liquid~ 2.0%；

Correction propyl alcohol: filling into n-butanol after the 20hr that ferments, and controls normal propyl alcohol content 0 .05~0 .10% in fermentation liquid.

The sodium nitrate solution mass-volume concentration is 15~25%, and glucose solution mass concentration is 20~30%, positive third Alcoholic solution mass concentration is 30~40%.

Trace element cobalt is added in the present invention in seed culture medium and fermentation medium, activates various enzymes in bacterial spawn, changes The metabolic capability and approach of thallus are become；The use for eliminating soybean cake powder in the fermentation medium fills into sodium nitrate solution work For nitrogen source, desaturation fermentation medium improves the oxygen supply condition in fermentation process, reduces the energy consumption of oxygen supply, thus Enhance thallus vigor, extend the erythromycin secretory phase, improves abomacetin fermentation level.The present invention passes through to seed and fermented and cultured The formulation composition of base changes, and the formulation composition and control mode of feed supplement change, and introduce trace element cobalt, to change thallus Metabolic pathway, second order fermentation is shorten to by the three grade fermemtation of original process, improves fermentation titer, shortens fermentation period, drop The low cost of raw material.Simultaneously because the reduction of effect carbon nitrogen source late, the extraction of erythromycin is more easy, and final product quality is also mentioned It is high.

Specific embodiment

Example is used below, the technical program is specifically described, it should be understood that example is for illustrating this hair It is bright rather than limiting the invention.The scope of the present invention is determined with core content according to claims.