阅读说明：本技术 一种青草纯露及其制备方法 (Green grass hydrolat and preparation method thereof ) 是由 李艳 李梓源 章文浩 孙学华 范冠宝 于 2021-07-31 设计创作，主要内容包括：本发明提供一种青草纯露及其制备方法,所述青草纯露每25kg水加入玫瑰花提取物120-140g、紫苏提取物90-110g、柑橘果皮提取物85-95g、枸杞提取物85-95g、鱼腥草提取物75-85g、降香提取物75-85g、积雪草提取物45-55g、香茅提取物38-43g和广藿香叶/茎提取物38-43g。本发明青草纯露,不但具有良好的美白、抗炎功效,而且具有一定烫伤修复功效。另外,本发明制备青草香露,以纯植物为原料,可适于长期使用。(The invention provides a green grass pure dew and a preparation method thereof, wherein 120-140g of rose extract, 90-110g of perilla extract, 85-95g of citrus peel extract, 85-95g of medlar extract, 75-85g of houttuynia cordata extract, 75-85g of dalbergia wood extract, 45-55g of asiatic centella extract, 38-43g of citronella extract and 38-43g of patchouli leaf/stem extract are added into 25kg of water. The green grass hydrolat has good whitening and anti-inflammatory effects and certain scald repair effect. In addition, the green grass balsam prepared by the invention takes pure plants as raw materials and can be suitable for long-term use.)

1. The green grass pure dew is characterized in that 140g of rose extract, 90-110g of perilla extract, 85-95g of citrus peel extract, 85-95g of medlar extract, 75-85g of houttuynia cordata extract, 75-85g of dalbergia wood extract, 45-55g of asiatic centella extract, 38-43g of citronella extract and 38-43g of patchouli leaf/stem extract are added into 25kg of water.

2. The grass hydrolat of claim 1, wherein 130g of rose extract, 100g of perilla extract, 90g of citrus peel extract, 90g of medlar extract, 80g of houttuynia cordata extract, 80g of dalbergia wood extract, 50g of centella asiatica extract, 40g of citronella extract and 40g of patchouli leaf/stem extract are added per 25kg of water.

3. The method for preparing the grass hydrolat according to claim 1 or 2, characterized by comprising the following steps: adding water accounting for 15-25% of the total amount of the prepared rosewood heart wood extract into a stirring pot, and hermetically soaking at 40-45 ℃; adding the extract of citrus peel and the extract of Chinese wolfberry, and stirring at 1200-1500r/min at 30-35 ℃ for 4-6 min; adding flos Rosae Rugosae extract and Perillae herba extract, and stirring at 20-25 deg.C for 4-6min at 500r/min at 300-; and finally, adding the balance of water, adding the houttuynia cordata extract, the centella asiatica extract, the citronella extract and the patchouli leaf/stem extract to obtain a primary mixed solution, and homogenizing in a homogenizer to obtain a homogenized solution, namely the grass hydrolat.

4. The preparation method of the grass hydrolat as claimed in claim 3, wherein the soaking time is 1-2 h.

5. The method for preparing herbal hydrolat according to claim 3 or 4, wherein the homogenization temperature is 25-40 ℃ and the homogenization pressure is 20-35 MPa.

6. The method for preparing herbal hydrolat according to claim 5, wherein the homogenization is performed according to the following procedure:

homogenization temperature (. degree. C.) Homogeneous pressure (MPa) Time of homogeneity (min) 25 35 2 35 25 2 40 20 2 35 25 2 25 35 2

Technical Field

The invention relates to the field of plant hydrolat, and particularly relates to green grass hydrolat and a preparation method thereof.

Background

The plant hydrolat is generally prepared as a byproduct in the process of extracting plant essential oil, is usually prepared simultaneously in the process of extracting the essential oil by water vapor, an oil-water mixed solution is formed by the action of condensed water, oil and water are separated according to different densities, the part of the separated oil layer is the plant essential oil, and the part of the water layer is the plant hydrolat. The plant hydrolat has natural and pure components, light and pleasant fragrance, is easy to be absorbed by skin, is mild and non-irritant, can be frequently used and has good market prospect. The plant hydrolat in the market is generally a single plant, mainly replenishes water and moisturizes, can be used as skin moisturizer for a long time and is a daily product, but other effects are not obvious or are not sufficient. The scald is inevitably sometimes burnt in daily life, but the scald repair product is not provided in the house, the scald repair product can not be treated in time, not only scars are easy to leave, but also the difficulty of complete repair of later medical and beauty is increased, and the skin appearance is influenced. Therefore, the plant hydrolat is developed, the daily skin lotion requirement can be met, and meanwhile, the scald can be repaired, so that the plant hydrolat has important significance.

Disclosure of Invention

In view of this, the invention provides a preparation method of the grass hydrolat, which solves the above problems.

The technical scheme of the invention is realized as follows: a green grass pure dew is prepared by adding 140g of rose extract 120-. The preferable mixture ratio is as follows: adding flos Rosae Rugosae extract 130g, Perillae herba extract 100g, Mandarin orange pericarp extract 90g, fructus Lycii extract 90g, herba Houttuyniae extract 80g, lignum Dalbergiae Odoriferae extract 80g, herba Centellae extract 50g, herba Cymbopogonis Citrari extract 40g, and folium Agastaches/stem extract 40g into 25kg water.

The preparation method of the green grass hydrolat comprises the following steps: adding water accounting for 15-25% of the total amount of the prepared rosewood heart wood extract into a stirring pot, and hermetically soaking at 40-45 ℃; adding the extract of citrus peel and the extract of Chinese wolfberry, and stirring at 1200-1500r/min at 30-35 ℃ for 4-6 min; adding flos Rosae Rugosae extract and Perillae herba extract, and stirring at 20-25 deg.C for 4-6min at 500r/min at 300-; and finally, adding the balance of water, adding the houttuynia cordata extract, the centella asiatica extract, the citronella extract and the patchouli leaf/stem extract to obtain a primary mixed solution, and homogenizing in a homogenizer to obtain a homogenized solution, namely the grass hydrolat.

Further, the soaking time is 1-2 hours.

Further, the homogenizing temperature is 25-40 ℃, the homogenizing pressure is 20-35MPa,

further, the homogenization is performed according to the following procedure:

homogenization temperature (. degree. C.)	Homogeneous pressure (MPa)	Time of homogeneity (min)
			25	35	2
35	25	2
			40	20	2
35	25	2
			25	35	2

Compared with the prior art, the invention has the beneficial effects that:

the rose extract, the perilla extract, the citrus peel extract, the medlar extract, the houttuynia cordata extract, the rosewood extract, the centella asiatica extract, the citronella extract and the patchouli leaf/stem extract are added for compounding, and the synergy is realized, so that the green grass fragrance hydrolat is prepared, and the green grass fragrance hydrolat not only has good whitening and anti-inflammatory effects, but also can promote the wound to heal quickly and has a certain scald repair effect. In addition, the preparation method adopts a specific stirring procedure and a gradient homogenization procedure, so that the product efficacy is further improved. The green grass flavor hydrolat prepared by the invention takes pure plants as raw materials and can be suitable for long-term use.

Detailed Description

In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.

The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.

The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.

Examples 1 to 4

(1) Formulation components

(2) Preparation process

S1, accurately weighing various raw materials in the formula, and placing the raw materials in a clean and sterilized utensil;

s2, putting the raw materials into a main pot, and uniformly stirring at room temperature to obtain a mixed solution;

s3, standing the obtained mixed solution, and discharging to obtain a semi-finished product;

and S4, sterilizing, inspecting, filling and packaging to obtain the finished product.

Example 5

The formulation was the same as in example 1. Adjusting the preparation process:

s1, accurately weighing various raw materials in the formula, and placing the raw materials in a clean and sterilized utensil;

s2, adding pure water with the total amount of 15% into a stirring pot, adding the dalbergia wood extract, and soaking for 1h in a sealed manner at the temperature of 45 ℃; adding the extract of citrus peel and the extract of Chinese wolfberry, and stirring at 35 deg.C for 5min at 1500 r/min; adding flos Rosae Rugosae extract and Perillae herba extract, and stirring at 25 deg.C for 5min at 500 r/min; adding the rest pure water, adding herba Houttuyniae extract, herba Centellae extract, herba Cymbopogonis Citrari extract, and folium Agastaches/stem extract to obtain a primary mixed solution, placing in a homogenizer,

homogenization temperature (. degree. C.)	Homogeneous pressure (MPa)	Time of homogeneity (min)
			25	35	2
35	25	2
			40	20	2
35	25	2
			25	35	2

Homogenizing according to the above procedure to obtain a homogenized liquid;

s3, standing the obtained homogeneous liquid, and discharging to obtain a semi-finished product;

and S4, sterilizing, inspecting, filling and packaging to obtain the finished product.

Example 6

The formulation was the same as in example 1. Adjusting the preparation process:

adding purified water with a total amount of 25% into a stirring pan, adding lignum Dalbergiae Odoriferae extract, and soaking at 40 deg.C under sealed condition for 2 hr; adding the extract of citrus peel and the extract of Chinese wolfberry, and stirring at 1200r/min and 30 ℃ for 5 min; adding flos Rosae Rugosae extract and Perillae herba extract, and stirring at 20 deg.C for 5min at 300 r/min; adding the rest pure water, adding herba Houttuyniae extract, herba Centellae extract, herba Cymbopogonis Citrari extract, and folium Agastaches/stem extract to obtain a primary mixed solution, placing in a homogenizer,

homogenization temperature (. degree. C.)	Homogeneous pressure (MPa)	Time of homogeneity (min)
			25	35	2
35	25	2
			40	20	2
35	25	2
			25	35	2

Homogenizing according to the above procedure to obtain a homogenized liquid;

s3, standing the obtained homogeneous liquid, and discharging to obtain a semi-finished product;

and S4, sterilizing, inspecting, filling and packaging to obtain the finished product.

Comparative example 1

(1) Recipe (adjusting recipe component dosage)

Serial number	Raw materials	Comparative example 1
			1	Water (W)	50 jin
2	Extract of flos Rosae Rugosae (ROSA RUGOSA) flower	50g
			3	Perilla (Perilla octaOIDES) extract	50g
4	Citrus (Citrus RETICULATA) pericarp extract	50g
			5	Fructus Lycii (Lycium CHINENSE) extract	130g
6	Herba Houttuyniae (HOUTTUYNIA CORDATA) extract	100g
			7	Lignum Dalbergiae Odoriferae (Dalbergia ODORIFERA) extract	60 g
8	Centella Asiatica (CENTELLAASIATICA) extract	90g
			9	Cymbopogoncitratus (Cymbopogoncitratus) extract	90g
10	Extract of leaf/stem of Pogostemon CABLIN	90g

(2) The preparation process was identical to example 1.

Comparative example 2

The main difference from example 1 is that the formulation does not contain dalbergia wood extract, citronella extract, dalbergia wood extract is replaced by an equal amount of centella asiatica extract, citronella extract is replaced by an equal amount of patchouli leaf/stem extract.

The formulation is given in the following table:

serial number	Raw materials	Example 1
			1	Pure water	50 jin
2	Extract of flos Rosae Rugosae (ROSA RUGOSA) flower	130g
			3	Perilla (Perilla octaOIDES) extract	100g
4	Citrus (Citrus RETICULATA) pericarp extract	90g
			5	Fructus Lycii (Lycium CHINENSE) extract	90g
6	Herba Houttuyniae (HOUTTUYNIA CORDATA) extract	80g
			7	Centella Asiatica (CENTELLAASIATICA) extract	130g
8	Extract of leaf/stem of Pogostemon CABLIN	80g

The preparation process was identical to example 1.

Test example 1 Scald repair efficacy test

Molding and administration: after the rats are raised for three days conventionally, the long hairs on the back are removed, then depilatory cream is coated, the hairs on the back area are removed after three minutes, and the rats are cleaned, wiped dry and are suitable for being fed for 24 hours. The rats in the experimental group are anesthetized with 10% chloral hydrate, the dosage of the anesthetized chloral hydrate is 0.3mL/100g, the unhairing area is sterilized by ethanol, 15mL of 100-DEG C hot water is injected into self-made centrifugal tubes with the diameter of 18mm at the positions of 1cm of the left side and the right side of a spinal column of the back of the rats respectively, the back of the rats is continuously scalded for 8s, symmetrical 2 round scald wounds with the depth of II degrees are prepared, the rats are kept warm, the rats are placed on the side, and the rats are prevented from being scaldedDeath was obtained by asphyxia. After the model building is successful, keeping conventional breeding, randomly dividing the rats into 9 groups, and enabling the rats to scald the model group: the medicine is not taken, and the iodine is coated for disinfection; test groups 1-7: the grass of examples 1-6 and comparative example 1 was sprayed with the herbal solution once a day, and the amount of each scald wound was about 0.05g/m²(ii) a A positive drug group: the ointment of Jingwanhong is externally applied once a day, and the dosage of each scald wound surface is about 0.05g/m². The rats in each group were observed at 3, 6, 11, 15, 21d and were obtained. Morphology observation of rats was performed by visual observation when rats were resuscitated after scald molding, wound surface state was observed 5, 9, 15, and 23 days after scald, and exudate, infection, scab, red swelling, etc. were recorded and photographed. The healing rate is mainly determined by taking the area of the 1 st day after molding as the original area and calculating the healing rate of the scald wound surface in 5, 9, 15 and 23 days.

The healing rate of the scald wound is (original scald area-non-healing area at different time points)/original scald area is multiplied by 100 percent

The results are as follows:

the results show that compared with the blank control, the herbal hydrolat prepared in examples 1-6 has better repairing effect on rat scalds, the 5d healing rate is 45-54%, the 9d healing rate is 55-64%, the 15d healing rate is 77-84%, and the 23d healing rate is 92-98%.

Test example 2 anti-inflammatory efficacy test

The green grass hydrolat prepared in examples 1-6 is adopted to respectively carry out tests of inhibiting NO release and inhibiting histamine release, wherein the NO release inhibiting capacity is 85-89%, and the histamine release inhibiting capacity is 80-84%, which shows that the green grass hydrolat prepared in examples 1-6 has good anti-inflammatory effect, and the green grass hydrolat prepared in examples 5-6 is obviously better than the green grass hydrolat prepared in examples 1-4.

Test example 3 whitening efficacy test

Tyrosinase activity inhibition assay and B16 cell melanin synthesis inhibition test were performed on the green grass hydrolat prepared in examples 1-6, respectively, and the inhibition rates for tyrosinase activity and B16 cell melanin synthesis were 89-91% and 78-80%, respectively, which shows that the green grass hydrolat prepared in examples 1-6 has excellent whitening effect, and the green grass hydrolat prepared in examples 5-6 is superior to the green grass hydrolat prepared in examples 1-4.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.