阅读说明：本技术 一种含橘类药材提取物的组合物及其制备方法和应用 (Composition containing orange medicinal material extract and preparation method and application thereof ) 是由 黄桦 于 2021-08-27 设计创作，主要内容包括：本发明公开了一种含橘类药材提取物的组合物及其制备方法和应用,包括如下重量份的组分：橘类药材提取物0.1-0.4份、西砒氯铵0.05-0.2份、防腐剂0.02-0.1份、壳寡糖0.2-0.4份、消泡剂0.3-0.5份、无菌水500份,本发明中添加的橘类药材提取物含有黄酮类化合物,黄酮类化合物具有很强的抑菌活性,其能够破坏微生物细胞壁及细胞膜的完整性,导致胞内成分释放而引起膜的电子传递、营养吸收、核苷酸合成及ATP活性等功能障碍,从而抑制微生物生长,对变形链球菌和白色念珠菌等均有抑制作用,同时壳寡糖与磷壁酸的静电相互作用破坏了磷壁酸的功能,造成了细菌的功能性紊乱,从而造成细菌死亡,橘类药材提取物和壳寡糖具有协同杀菌的作用。(The invention discloses a composition containing an orange medicinal material extract, a preparation method and an application thereof, wherein the composition comprises the following components in parts by weight: 0.1 to 0.4 portion of orange medicinal material extract, 0.05 to 0.2 portion of arsenic trioxide, 0.02 to 0.1 portion of preservative, 0.2 to 0.4 portion of chitosan oligosaccharide, 0.3 to 0.5 portion of defoaming agent and 500 portions of sterile water, wherein the orange medicinal material extract added in the invention contains flavonoid compounds which have strong bacteriostatic activity, it can destroy the integrity of microbial cell wall and cell membrane, and release intracellular components to cause dysfunction of membrane in electron transfer, nutrient absorption, nucleotide synthesis and ATP activity, so as to inhibit microbial growth, has inhibiting effect on Streptococcus mutans and Candida albicans, etc., and the electrostatic interaction between chitosan oligosaccharide and teichoic acid destroys the function of teichoic acid, causes functional disorder of bacteria, thereby causing bacterial death, and the tangerine extract and the chitosan oligosaccharide have synergistic bactericidal effect.)

1. A mouthwash containing an orange medicinal material extract is characterized by comprising the following components in parts by weight: 0.1-0.4 part of orange medicinal material extract, 0.05-0.2 part of arsenic trioxide, 0.02-0.1 part of preservative, 0.2-0.4 part of chitosan oligosaccharide, 0.3-0.5 part of defoaming agent and 500 parts of sterile water;

the extraction process of the orange medicinal material extract comprises the following steps: adding orange medicinal materials into an extraction kettle, then adding entrainer absolute ethyl alcohol accounting for 10-30% of the addition amount of the orange medicinal materials, extracting at 22MPa and 35 ℃, and separating the extract through a separation kettle after the extraction is finished to obtain the orange medicinal material extract.

2. The mouthwash containing the orange extract as claimed in claim 1, is characterized by comprising the following components in parts by weight: 0.2 part of orange medicinal material extract, 0.1 part of cetylpyridinium chloride, 0.05 part of preservative, 0.3 part of chitosan oligosaccharide, 0.4 part of defoaming agent and 500 parts of sterile water.

3. The mouthwash containing the orange extract according to claim 1, wherein the orange extract is one or more of a tangerine peel extract, a citrus aurantium extract and a citrus aurantium extract.

4. The mouthwash containing orange extract according to claim 1, wherein the preservative is one or more of sodium benzoate, methyl paraben and ethyl paraben.

5. The mouthwash containing orange extract according to claim 1, wherein the antifoaming agent is a solution containing 0.8 wt% of polydimethylsiloxane.

6. The preparation method of the mouthwash containing orange extract according to any one of claims 1 to 5, is characterized by comprising the following preparation steps: adding arsenic trioxide and a preservative into absolute ethyl alcohol, stirring and dissolving, adding 250 parts of sterile water, uniformly dispersing by ultrasonic, then adding the orange medicinal material extract, chitosan oligosaccharide and a defoaming agent, adding 250 parts of sterile water, and uniformly dispersing by ultrasonic to obtain the mouthwash containing the orange medicinal material extract.

7. The application of the orange medicinal material extract in preparing the vaginal lotion is characterized in that the vaginal lotion is prepared from the following components in parts by weight: 0.4 part of orange medicinal material extract, 0.2 part of cetylpyridinium chloride, 0.1 part of preservative, 0.3 part of chitosan oligosaccharide, 0.4 part of defoaming agent and 500 parts of sterile water.

8. The application of the orange medicinal material extract in preparing the disinfectant is characterized in that the disinfectant is prepared from the following components in parts by weight: 0.4 part of orange medicinal material extract, 0.2 part of cetylpyridinium chloride, 0.1 part of preservative, 0.3 part of chitosan oligosaccharide, 0.4 part of defoaming agent and 500 parts of sterile water.

9. The use of claim 7 or 8, wherein the citrus extract is one or more of a citrus peel extract, a citrus aurantium extract and a citrus aurantium extract.

Technical Field

The invention belongs to the technical field of preparation and application of extracts of orange medicinal materials, and particularly relates to a composition containing extracts of orange medicinal materials, and a preparation method and application thereof.

Background

The mouthwash is an indispensable oral cavity cleaning sanitary product in daily life of people, and has the basic functions of cleaning teeth, refreshing breath and achieving the purpose of keeping the oral cavity clean. In recent years, as health care consciousness of consumers is enhanced, more requirements are put forward on mouthwash, besides tooth cleaning, a plurality of functions for assisting in preventing oral diseases and nursing the oral cavity are added, so that products with various functions in the mouthwash at home and abroad become the mainstream of the market, and as the active ingredients of traditional Chinese medicines and plants have the advantages that the active ingredients of the traditional Chinese medicines are natural, safe and the like, which cannot be compared with western medicines, the functional mouthwash containing the active ingredients of the traditional Chinese medicines in the market becomes the key point of the current development. In recent years, with the development of the modernization technology of traditional Chinese medicines, a method for researching and utilizing active ingredients of traditional Chinese medicines is well developed, and good technical support is provided for assisting in preventing oral diseases by utilizing the active ingredients of traditional Chinese medicines in mouthwash.

Patent document (CN201310208493.3) discloses a mouthwash containing panax notoginseng extract, which comprises the following components in percentage by weight: 0.1-1.5% of pseudo-ginseng extract, 12-35% of humectant, 0.8-2.5% of surfactant, 0.01-0.5% of sweetening agent, 0.5-1% of flavoring agent, 0.01-0.15% of preservative, 0.1-0.2% of sodium pyrophosphate, 0.05-0.15% of sodium fluoride and the balance of deionized water.

Patent document (CN201410425062.7) discloses a mouthwash containing enzymes and a dried orange peel extract, wherein the dosage relationship of the substances in the mouthwash is that every 100mL of mouthwash consists of 2.5-10 mL of dried orange peel extract, 0.25-10 mg of alpha-glucanase, 0.25-10 mg of beta-glucanase, 0.25-10 mg of alpha-amylase, 2.5-5 mL of mint extract, 1g of salt and the balance of water.

Patent document (CN201810224269.6) discloses a multi-effect mouthwash, which comprises the following components in parts by weight: 2.8-3.6 parts of solubilizer, 2-3 parts of glycerol, 0.8-1.1 parts of efficacy additive, 0.3-0.4 part of preservative, 18.4-20.3 parts of taste modifier and the balance of water, the invention adopts a method of compounding PEG-40 hydrogenated castor oil and polysorbate-20, the dosage of the method can be reduced to 0.2-0.5%, and the problem of poor taste is solved; the propylene glycol is used for assisting capacity increasing and improving transparency, the menthol for assisting cooling is added, fresh taste is brought without stimulating oral cavity, in the aspect of sweet taste, the sorbitol and the xylitol are used, the trichlorogalactose is added for coordinating the compound method, the traditional sweetening agent saccharin sodium is abandoned, the defect that the flavor is poor and bitter is caused is overcome, pleasant and suitable taste experience is brought, and finally, the taste of the mouthwash is improved on the whole, and the customer experience is improved.

At present, although the traditional Chinese medicine active ingredients are utilized to assist in preventing oral diseases, the problems of dark color, poor taste, much foam, poor antibacterial performance and the like of the mouthwash still exist.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a composition containing an orange medicinal material extract, a preparation method and an application thereof, and solves the technical problems of more foams and poor antibacterial performance of the existing mouthwash.

In order to achieve the purpose, the invention adopts the following technical scheme:

a mouthwash containing orange medicinal material extracts comprises the following components in parts by weight: 0.1-0.4 part of orange medicinal material extract, 0.05-0.2 part of arsenic trioxide, 0.02-0.1 part of preservative, 0.2-0.4 part of chitosan oligosaccharide, 0.3-0.5 part of defoaming agent and 500 parts of sterile water;

the extraction process of the orange medicinal material extract comprises the following steps: adding orange medicinal materials into an extraction kettle, then adding entrainer absolute ethyl alcohol accounting for 10-30% of the addition amount of the orange medicinal materials, extracting at 22MPa and 35 ℃, and separating the extract through a separation kettle after the extraction is finished to obtain the orange medicinal material extract.

Preferably, the composition comprises the following components in parts by weight: 0.2 part of orange medicinal material extract, 0.1 part of cetylpyridinium chloride, 0.05 part of preservative, 0.3 part of chitosan oligosaccharide, 0.4 part of defoaming agent and 500 parts of sterile water.

Preferably, the orange medicinal material extract is one or more of dried orange peel extract, immature bitter orange extract and bitter orange extract.

Preferably, the preservative is one or more of sodium benzoate, methyl paraben and ethyl paraben.

Preferably, the defoamer is a solution containing 0.8 wt% polydimethylsiloxane.

The invention provides a preparation method of mouthwash containing orange medicinal material extracts, which is characterized by comprising the following preparation steps: adding arsenic trioxide and a preservative into absolute ethyl alcohol, stirring and dissolving, adding 250 parts of sterile water, uniformly dispersing by ultrasonic, then adding the orange medicinal material extract, chitosan oligosaccharide and a defoaming agent, adding 250 parts of sterile water, and uniformly dispersing by ultrasonic to obtain the mouthwash containing the orange medicinal material extract.

The invention also provides an application of the tangerine medicinal material extract in preparing vaginal lotion, which comprises the following components in parts by weight: 0.4 part of orange medicinal material extract, 0.2 part of cetylpyridinium chloride, 0.1 part of preservative, 0.3 part of chitosan oligosaccharide, 0.4 part of defoaming agent and 500 parts of sterile water.

The invention provides an application of an orange medicinal material extract in preparing a disinfectant, which comprises the following components in parts by weight: 0.4 part of orange medicinal material extract, 0.2 part of cetylpyridinium chloride, 0.1 part of preservative, 0.3 part of chitosan oligosaccharide, 0.4 part of defoaming agent and 500 parts of sterile water.

Compared with the prior art, the invention has the following beneficial effects:

(1) the invention provides a composition containing an orange medicinal material extract, a preparation method and an application thereof, wherein chitosan oligosaccharide and a defoaming agent added in the composition have a synergistic defoaming effect, polydimethylsiloxane is a defoaming agent with better performance but has poorer hydrophilicity, the negative charge of the polydimethylsiloxane strongly adsorbs chitosan oligosaccharide with positive charge, and the chitosan oligosaccharide is easy to dissolve in water, so that the hydrophilic performance of the defoaming agent is improved, the chitosan oligosaccharide is easy to spread in a foaming medium, and the defoaming capability is enhanced.

(2) The invention provides a composition containing orange medicinal extract and a preparation method and application thereof, the orange medicinal extract and chitosan oligosaccharide added in the composition have a synergistic sterilization effect, the orange medicinal extract contains flavonoid compounds, the flavonoid compounds have strong bacteriostatic activity and can destroy the integrality of microbial cell walls and cell membranes, so that intracellular components are released to cause functional disorders such as electronic transmission, nutrient absorption, nucleotide synthesis, ATP activity and the like of membranes, thereby inhibiting the growth of microorganisms, inhibiting streptococcus mutans, candida albicans and the like, simultaneously, cationic charges in a chitosan oligosaccharide skeleton have an important effect on the antibacterial activity, and the electrostatic interaction of the chitosan oligosaccharide and the teichoic acid destroys the function of the teichoic acid, causes the functional disorder of bacteria, thereby causing the death of the bacteria, the composition prepared by the invention is suitable for cleaning and disinfecting the vagina, and can also be used as disinfectant.

(3) The orange medicinal material extract can be applied to mouthwash, vaginal lotion and disinfectant, has excellent sterilization and disinfection effects, and expands the application range of the orange medicinal material extract.

Detailed Description

The present invention will be described in more detail with reference to specific preferred embodiments, but the present invention is not limited to the following embodiments.

It should be noted that, unless otherwise specified, the chemical reagents involved in the present invention are commercially available.

The orange medicinal material is selected from tangerine peel, the tangerine peel is purchased from Hunan Zhenxing decoction piece industry limited company, and the extraction process of the tangerine peel extract comprises the following steps: weighing 600g of dried orange peel, adding the dried orange peel into an extraction kettle, adding 120mL of ethanol as an entrainer, extracting for 2 hours at the temperature of 35 ℃ under the pressure of 22MPa, and then separating by a separation kettle to obtain the dried orange peel extract.

Example 1

A preparation method of mouthwash containing dried orange peel extract comprises the following steps:

adding 0.1g of cetylpyridinium chloride and 0.02g of antiseptic methyl paraben into 10mL of absolute ethyl alcohol, stirring for dissolving, adding 250g of sterile water, uniformly dispersing by ultrasonic, then adding 0.1g of dried orange peel extract, 0.2g of chitosan oligosaccharide, 0.3g of solution containing 0.8 wt% of polydimethylsiloxane, adding 250g of sterile water, and uniformly dispersing by ultrasonic to obtain the mouthwash containing the dried orange peel extract.

Example 2

A preparation method of mouthwash containing dried orange peel extract comprises the following steps:

adding 0.1g of cetylpyridinium chloride and 0.05g of antiseptic methyl paraben into 10mL of absolute ethyl alcohol, stirring for dissolving, adding 250g of sterile water, uniformly dispersing by ultrasonic, then adding 0.2g of dried orange peel extract, 0.3g of chitosan oligosaccharide, 0.4g of solution containing 0.8 wt% of polydimethylsiloxane, adding 250g of sterile water, and uniformly dispersing by ultrasonic to obtain the mouthwash containing the dried orange peel extract.

Example 3

A preparation method of mouthwash containing dried orange peel extract comprises the following steps:

adding 0.15g of cetylpyridinium chloride and 0.05g of antiseptic methyl paraben into 10mL of absolute ethyl alcohol, stirring for dissolving, adding 250g of sterile water, uniformly dispersing by ultrasonic, then adding 0.3g of dried orange peel extract, 0.35g of chitosan oligosaccharide, 0.4g of solution containing 0.8 wt% of polydimethylsiloxane, adding 250g of sterile water, and uniformly dispersing by ultrasonic to obtain the mouthwash containing the dried orange peel extract.

Example 4

A preparation method of mouthwash containing dried orange peel extract comprises the following steps:

adding 0.2g of cetylpyridinium chloride and 0.08g of antiseptic methyl paraben into 10mL of absolute ethyl alcohol, stirring for dissolving, adding 250g of sterile water, uniformly dispersing by ultrasonic, then adding 0.4g of dried orange peel extract, 0.35g of chitosan oligosaccharide, 0.4g of solution containing 0.8 wt% of polydimethylsiloxane, adding 250g of sterile water, and uniformly dispersing by ultrasonic to obtain the mouthwash containing the dried orange peel extract.

Comparative example 1

A preparation method of mouthwash comprises the following steps:

adding 0.2g of cetylpyridinium chloride and 0.08g of antiseptic methyl paraben into 10mL of absolute ethyl alcohol, stirring for dissolving, adding 250g of sterile water, uniformly dispersing by ultrasonic, then adding 0.35g of chitosan oligosaccharide and 0.4g of solution containing 0.8 wt% of polydimethylsiloxane, adding 250g of sterile water, and uniformly dispersing by ultrasonic to obtain the mouthwash containing the dried orange peel extract.

Comparative example 2

A preparation method of mouthwash containing dried orange peel extract comprises the following steps:

adding 0.2g of cetylpyridinium chloride and 0.08g of antiseptic methyl-p-hydroxybenzoate into 10mL of absolute ethyl alcohol, stirring for dissolving, adding 250g of sterile water, uniformly dispersing by ultrasonic, adding 0.4g of dried orange peel extract and 0.4g of solution containing 0.8 wt% of polydimethylsiloxane, adding 250g of sterile water, and uniformly dispersing by ultrasonic to obtain the mouthwash containing the dried orange peel extract.

20mL of the mouth rinses prepared in the examples 1-4 and the comparative example 2 are respectively put into a shaker to be shaken for 2min, and after the shaking is finished, whether foam is generated or not is observed, and the experimental results are shown in the following table:

item	Example 1	Example 2	Example 3	Example 4	Comparative example 2
						Generating foam conditions	Non-foam	Non-foam	Non-foam	Non-foam	With foam

As can be seen from the table, the mouthwash prepared in this example generates no foam after being shaken, and the chitosan oligosaccharide is not added in comparative example 2, and the prepared mouthwash generates foam after being shaken, which indicates that the addition of chitosan oligosaccharide is beneficial to reducing the generation of foam.

The antibacterial property test is carried out on the mouth rinses prepared in the examples 1-4 and the comparative examples 1-2, the blank control group selects physiological saline with the mass fraction of 0.9%, and the specific test steps are as follows:

0.5mL of the mouth rinse prepared in examples 1 to 4 and comparative examples 1 to 2 was dropped into a volume of 10mL of a medium (surface area of 10 cm)²3% tryptose Soy Broth mixed with agarSolid agar), inoculating candida albicans, streptococcus mutans and staphylococcus aureus respectively, placing the inoculated candida albicans, streptococcus mutans and staphylococcus aureus in a constant-temperature incubator at 37 ℃ for culturing for 24 hours, forming a circular antibacterial ring on the surface of a culture medium, and measuring the diameter of the antibacterial ring (unit: mm), the experimental results are shown in the following table:

as can be seen from the table, the mouth wash prepared in this example has good antibacterial effects on candida albicans, streptococcus mutans and staphylococcus aureus, the tangerine peel extract is not added in comparative example 1, and the chitosan oligosaccharide is not added in comparative example 2, although the mouth wash has a certain antibacterial effect, the effect is inferior to that of this example, and the chitosan oligosaccharide and the tangerine peel extract have a synergistic bactericidal effect.

Finally, it is to be noted that: the above examples do not limit the invention in any way. It will be apparent to those skilled in the art that various modifications and improvements can be made to the present invention. Accordingly, any modification or improvement made without departing from the spirit of the present invention is within the scope of the claimed invention.