阅读说明：本技术 一种禽用中药组合物及其制备方法和应用 (Traditional Chinese medicine composition for poultry and preparation method and application thereof ) 是由 刘澜 卢贵梅 黄灵妍 于 2021-08-04 设计创作，主要内容包括：本发明公开了一种禽用中药组合物,涉及兽用中药领域。该中药组合物含有活性成分,所述活性成分由：黄芪、党参、麦冬、五味子、岩豇豆、鸡屎藤、青蒿、甘露寡糖制成。本发明药物组合物具有益气固精、扶正培元、生津补虚、止咳平喘之功效,适用于动物增强免疫力、预防疾病。本发明药物组合物组方精当、疗效确切、安全性高、制剂稳定、质量可控、制备工艺简单、适合工业化生产等特点。(The invention discloses a Chinese medicinal composition for poultry, and relates to the field of veterinary Chinese medicaments. The traditional Chinese medicine composition contains active ingredients, wherein the active ingredients comprise: radix astragali, radix Codonopsis, radix Ophiopogonis, fructus Schisandrae chinensis, herba Cissampelotis Pareiae, herba Paederiae, herba Artemisiae Annuae, and mannooligosaccharide. The pharmaceutical composition has the effects of tonifying qi and securing essence, strengthening body resistance and cultivating vigor, promoting the production of body fluid and tonifying deficiency, and relieving cough and asthma, and is suitable for enhancing the immunity of animals and preventing diseases. The pharmaceutical composition has the characteristics of precise and appropriate formula, definite curative effect, high safety, stable preparation, controllable quality, simple preparation process, suitability for industrial production and the like.)

1. A Chinese medicinal composition for poultry contains active ingredients, and is characterized in that the active ingredients are prepared from astragalus, codonopsis pilosula, dwarf lilyturf tuber, Chinese magnoliavine fruit, rock cowpea, Chinese fevervine herb, sweet wormwood herb and mannan oligosaccharide.

2. The poultry traditional Chinese medicine composition as claimed in claim 1, wherein the active ingredients are prepared from 20-70 parts of astragalus membranaceus, 10-50 parts of codonopsis pilosula, 10-40 parts of radix ophiopogonis, 5-25 parts of schisandra chinensis, 5-20 parts of rock cowpea, 10-30 parts of paederia scandens, 8-26 parts of artemisia apiacea and 70-100 parts of mannan oligosaccharide.

3. The poultry traditional Chinese medicine composition as claimed in claim 2, wherein the active ingredients are prepared from 30-60 parts of astragalus, 20-40 parts of codonopsis pilosula, 20-35 parts of radix ophiopogonis, 10-20 parts of schisandra chinensis, 8-16 parts of rock cowpea, 15-25 parts of paederia scandens, 12-22 parts of sweet wormwood herb and 80-90 parts of mannan oligosaccharide.

4. The avian traditional Chinese medicine composition according to claim 3, wherein the active ingredients consist of: 50 parts of astragalus, 30 parts of codonopsis pilosula, 28 parts of dwarf lilyturf tuber, 16 parts of Chinese magnoliavine fruit, 15 parts of rock cowpea, 20 parts of Chinese fevervine herb, 18 parts of sweet wormwood herb and 85 parts of mannan oligosaccharide.

5. A method for preparing the poultry Chinese medicinal composition as claimed in any one of claims 1 to 4, which comprises the following steps:

(1) the raw materials are respectively weighed according to the weight ratio.

(2) Pulverizing the above Chinese medicinal materials except mannooligosaccharide, decocting with 10-12 times of water for 2-3 times (each for 1-2 hr), filtering, mixing filtrates, and concentrating under reduced pressure to obtain concentrated extract (1g is 3g of crude drug).

(3) And (3) adding weighed mannan oligosaccharide into the thick extract obtained in the step (2), uniformly mixing, adding sucrose and dextrin, uniformly mixing, and preparing into granules.

6. The preparation method of the poultry traditional Chinese medicine composition according to claim 5, wherein in the step 3, the adding ratio of the sucrose to the dextrin is 1: 1.

7. use of the poultry Chinese medicinal composition as defined in any one of claims 1-4 in preparing medicament for enhancing poultry immunity and disease resistance.

8. Use according to claim 7, wherein the avian is a vaccinated avian.

9. The use according to claim 8, wherein the vaccination is a newcastle disease vaccine, an avian influenza vaccine.

10. A premix, concentrate, or batch comprising the avian herbal composition of any one of claims 1 to 4.

Technical Field

The invention relates to the field of veterinary traditional Chinese medicines, and in particular relates to a traditional Chinese medicine composition for poultry and a preparation method and application thereof.

Background

The intensive large-scale breeding mode ensures that livestock and poultry are raised in the environment with high nutrition, high density, strong stress and over-speed growth without burden, so that the livestock and poultry are low in immunity, weak in disease resistance and greatly increased in disease susceptibility, and particularly under the coexistence of factors such as excessive immunity, mycotoxin, heavy metal pollution, increase in immunosuppressive diseases, large dose of chemical drugs and the like, the immune system of the animal body is seriously damaged, diseases are abused frequently, diseases are outbreak, a large amount of livestock and poultry die, old diseases are continuous, new diseases occur, and great harm is brought to the development of the breeding industry. Even if livestock and poultry which are reluctant to survive are mostly in a sub-health state for a long time, in order to enable the livestock and poultry to get rid of the sub-health state and maintain the high-speed growth and reproductive capacity of the livestock and poultry, the breeding personnel have to use a large amount of medicines, vaccines and chemical additives, which greatly increases the risk of food safety hidden dangers. Therefore, the development of green, safe and efficient medicaments for enhancing the immunity of the organism and improving the disease resistance is not slow enough.

The traditional Chinese medicine has unique advantages in enhancing the immunity of organisms and improving the disease resistance of animals, and becomes the most important field of the current research. Most people in the field usually only consider the medicine for enhancing immunity when selecting the immunity enhancing medicine, but neglecting that the animal is an organic whole, and under the condition that the animal has diseases, the animal cannot completely eliminate the diseases even if the immunity of the animal body is improved, and the traditional medicine only considers the aspect of enhancing the immunity of the animal body but neglects the aspect of disease resistance. During the process of disease occurrence and development, there are always strength contrast between "healthy qi" and "pathogenic qi" and the changes of growth and weakness, and the development and prognosis of disease are directly influenced.

Disclosure of Invention

In order to solve the technical problems, the invention aims to provide a safe and efficient poultry traditional Chinese medicine composition.

The purpose of the invention is realized by the following technical scheme:

the invention provides a Chinese medicinal composition for poultry, which contains active ingredients, wherein the active ingredients comprise: radix astragali, radix Codonopsis, radix Ophiopogonis, fructus Schisandrae chinensis, herba Cissampelotis Pareiae, herba Paederiae, herba Artemisiae Annuae, and mannooligosaccharide.

Furthermore, the active ingredients are prepared from 20-70 parts of astragalus root, 10-50 parts of codonopsis pilosula, 10-40 parts of dwarf lilyturf tuber, 5-25 parts of Chinese magnoliavine fruit, 5-20 parts of rock cowpea, 10-30 parts of Chinese fevervine herb, 8-26 parts of sweet wormwood herb and 70-100 parts of mannan oligosaccharide by weight.

Further, the active ingredients are prepared from 30-60 parts by weight of astragalus, 20-40 parts by weight of codonopsis pilosula, 20-35 parts by weight of radix ophiopogonis, 10-20 parts by weight of schisandra chinensis, 8-16 parts by weight of rock cowpea, 15-25 parts by weight of paederia scandens, 12-22 parts by weight of sweet wormwood herb and 80-90 parts by weight of mannan oligosaccharide.

Furthermore, the active ingredients are prepared from 50 parts by weight of astragalus, 30 parts by weight of codonopsis pilosula, 28 parts by weight of radix ophiopogonis, 16 parts by weight of schisandra chinensis, 15 parts by weight of rock cowpea, 20 parts by weight of Chinese fevervine herb, 18 parts by weight of sweet wormwood herb and 85 parts by weight of mannan oligosaccharide.

The invention also provides a preparation method of the poultry traditional Chinese medicine composition, which comprises the following steps:

(1) the raw materials are respectively weighed according to the weight ratio.

(2) Pulverizing the above Chinese medicinal materials except mannooligosaccharide, decocting with 10-12 times of water for 2-3 times (each for 1-2 hr), filtering, mixing filtrates, and concentrating under reduced pressure to obtain concentrated extract (1g is 3g of crude drug).

(3) And (3) adding weighed mannan oligosaccharide into the thick extract obtained in the step (2), uniformly mixing, adding sucrose and dextrin (the ratio of the sucrose to the dextrin is 1: 1), uniformly mixing, and preparing into granules.

The poultry Chinese medicinal composition can be used for enhancing the immunity of organisms and improving the disease resistance of poultry.

In particular to a medicine which is prepared from the poultry Chinese medicinal composition and is used for enhancing the immunity of poultry organisms and improving disease resistance. Preferably, the avian is an already vaccinated avian; preferably, the vaccination vaccine is a newcastle disease vaccine and an avian influenza vaccine.

The invention also provides a premix, a concentrated material and a batch containing the poultry traditional Chinese medicine composition.

Compared with the prior art, the traditional Chinese medicine composition has the beneficial effects that:

the traditional Chinese medicine composition is prepared by carrying out repeated verification and practice on various medicinal components and the dosage thereof according to the dialectical theory of Chinese veterinary medicine formulas and a large number of tests.

The traditional Chinese medicine holds that the occurrence of any disease is the result of the struggle between vital qi and pathogenic factors under certain conditions. The pathogenic factors must be removed from the body, the pathogenic factors must be weakened, and the pathogenic factors must be weakened, so that the strength contrast and the growth and decline of the pathogenic factors will always exist in the process of disease occurrence and development, and the development and prognosis of the disease are directly influenced. The defensive ability of healthy qi is manifested in resisting invasion of exogenous pathogenic factors, expelling pathogenic factors, restoring and regulating ability, and maintaining coordination of viscera and meridians. The invasion of pathogenic qi is mainly manifested by physiological dysfunction, physical damage to viscera and tissues, and changes of constitutions and types. When healthy qi is larger than pathogenic qi, the body is in a healthy state, otherwise in a sick state. Therefore, improving immunity and disease resistance should improve healthy qi of the body. The therapeutic principle should be strengthening healthy qi and eliminating pathogens, and the strengthening healthy qi and eliminating pathogens should be closely linked. Strengthening the body resistance is to strengthen the resistance of the animal body to overcome pathogenic factors; the elimination of pathogenic factors is to maintain healthy qi, i.e., to eliminate pathogenic factors, so as to restore the qi and blood of viscera of the animal body to normal. The vital qi in veterinary medicine includes the functional activities of the viscera, tissues and organs, the resistance of the body to environmental stress and the resistance of the body to the specificity of pathogenic factors. In order to achieve the aim of strengthening the body resistance, which is known to veterinarians, in one aspect, the functional activities of viscera, tissues and organs must be enhanced; to enhance the functional activities of the zang-fu organs, tissues and organs, the yang qi must be tonified. For example, spleen qi and kidney yang.

In the invention, the astragalus and the codonopsis pilosula can promote the production of body fluid, recover pulse and greatly tonify primordial qi, and are used as monarch drugs; the radix ophiopogonis is used as a ministerial drug for nourishing yin and promoting the production of body fluid and increasing the effects of nourishing yin and promoting the production of body fluid and tonifying qi by being matched with the radix codonopsis and the radix astragali; wu Wei Zi can astringe yin to check sweating, promote the production of body fluid and replenish qi, and is good at astringing the dissipating real qi. The rock cowpea disperses wind to relieve cough, digests food and removes food retention, and is used as adjuvant drug together; the Chinese fevervine herb has the functions of dispelling wind, activating blood, relieving pain, detoxifying, promoting digestion, removing food stagnation, eliminating dampness, reducing swelling, tonifying consumptive disease, regulating spleen and stomach primordial qi and reducing heat stress. Sweet wormwood clears deficiency heat, cools blood, removes heat from the blood, removes dampness, relieves summer heat, intercepts malaria, can improve the conversion rate of lymphocytes, promotes the immunity of body cells, has the functions of bacteriostasis and disinsection, and is a guiding drug together. The medicines are combined together to have the effects of nourishing yin, promoting the production of body fluid, tonifying qi, activating blood circulation, promoting digestion, removing food stagnation and enhancing immunity.

According to the invention, the mannan oligosaccharide is added on the basis of the traditional Chinese medicine composition, and the mannan oligosaccharide are combined for use through experiments, so that the antibody level of the newcastle disease vaccine inoculated chicks can be further improved. Meanwhile, tests prove that compared with the existing products, the traditional Chinese medicine composition provided by the invention has excellent effects in the aspects of improving the poultry antibody level and preventing poultry diseases.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

Example 1

(1) Weighing the following Chinese medicinal materials in parts by weight: 20 parts of astragalus membranaceus, 10 parts of codonopsis pilosula, 10 parts of radix ophiopogonis, 5 parts of schisandra chinensis, 5 parts of rock cowpea, 10 parts of Chinese fevervine herb, 8 parts of sweet wormwood herb and 70 parts of mannan oligosaccharide.

(2) Pulverizing the above Chinese medicinal materials except mannooligosaccharide, decocting with 10-12 times of water for 2-3 times (each for 1-2 hr), filtering, mixing filtrates, and concentrating under reduced pressure to obtain concentrated extract (1g is 3g of crude drug).

(3) And (3) adding weighed mannan oligosaccharide into the thick extract obtained in the step (2), uniformly mixing, adding sucrose and dextrin (the ratio of the sucrose to the dextrin is 1: 1), uniformly mixing, and preparing into granules.

Example 2

(1) Weighing the following Chinese medicinal materials in parts by weight: 70 parts of astragalus, 50 parts of codonopsis pilosula, 40 parts of dwarf lilyturf tuber, 25 parts of Chinese magnoliavine fruit, 20 parts of rock cowpea, 30 parts of Chinese fevervine herb, 26 parts of sweet wormwood herb and 100 parts of mannan oligosaccharide

(2) Pulverizing the above Chinese medicinal materials except mannooligosaccharide, decocting with 10-12 times of water for 2-3 times (each for 1-2 hr), filtering, mixing filtrates, and concentrating under reduced pressure to obtain concentrated extract (1g is 3g of crude drug).

(3) And (3) adding weighed mannan oligosaccharide into the thick extract obtained in the step (2), uniformly mixing, adding sucrose and dextrin (the ratio of the sucrose to the dextrin is 1: 1), uniformly mixing, and preparing into granules.

Example 3

(1) Weighing the following Chinese medicinal materials in parts by weight: 30 parts of astragalus, 20 parts of codonopsis pilosula, 20 parts of dwarf lilyturf tuber, 10 parts of Chinese magnoliavine fruit, 8 parts of rock cowpea, 15 parts of Chinese fevervine herb, 12 parts of sweet wormwood herb and 80 parts of mannan oligosaccharide

(2) Pulverizing the above Chinese medicinal materials except mannooligosaccharide, decocting with 10-12 times of water for 2-3 times (each for 1-2 hr), filtering, mixing filtrates, and concentrating under reduced pressure to obtain concentrated extract (1g is 3g of crude drug).

(3) And (3) adding weighed mannan oligosaccharide into the thick extract obtained in the step (2), uniformly mixing, adding sucrose and dextrin (the ratio of the sucrose to the dextrin is 1: 1), uniformly mixing, and preparing into granules.

Example 4

(1) Weighing the following Chinese medicinal materials in parts by weight: 60 parts of astragalus, 40 parts of codonopsis pilosula, 35 parts of dwarf lilyturf tuber, 20 parts of Chinese magnoliavine fruit, 16 parts of rock cowpea, 25 parts of Chinese fevervine herb, 22 parts of sweet wormwood herb and 90 parts of mannan oligosaccharide

(2) Pulverizing the above Chinese medicinal materials except mannooligosaccharide, decocting with 10-12 times of water for 2-3 times (each for 1-2 hr), filtering, mixing filtrates, and concentrating under reduced pressure to obtain concentrated extract (1g is 3g of crude drug).

(3) And (3) adding weighed mannan oligosaccharide into the thick extract obtained in the step (2), uniformly mixing, adding sucrose and dextrin (the ratio of the sucrose to the dextrin is 1: 1), uniformly mixing, and preparing into granules.

Example 5

(1) Weighing the following Chinese medicinal materials in parts by weight: 50 parts of astragalus membranaceus, 30 parts of codonopsis pilosula, 28 parts of radix ophiopogonis, 16 parts of schisandra chinensis, 15 parts of rock cowpea, 20 parts of Chinese fevervine herb, 18 parts of sweet wormwood herb and 85 parts of mannan oligosaccharide.

(2) Pulverizing the above Chinese medicinal materials except mannooligosaccharide, decocting with 10-12 times of water for 2-3 times (each for 1-2 hr), filtering, mixing filtrates, and concentrating under reduced pressure to obtain concentrated extract (1g is 3g of crude drug).

(3) And (3) adding weighed mannan oligosaccharide into the concentrated extract obtained in the step (2), uniformly mixing, adding sucrose and dextrin (the ratio of the sucrose to the dextrin is 1: 1), uniformly mixing, and preparing into granules (1g of granules is equivalent to 3g of crude drug).

Comparative example 1

A Chinese herbal medicine immunopotentiator for chicken, which can improve the immunity of chicken and enhance the disease resistance and disease prevention capability, comprises the following components in parts by weight: 40 parts of echinacea purpurea, 20 parts of astragalus membranaceus, 10 parts of rhodiola rosea, 20 parts of codonopsis pilosula and 10 parts of liquorice.

The preparation method of the Chinese herbal medicine immunopotentiator for the chicken, which can improve the immunity of the chicken and enhance the disease resistance and disease prevention capability, comprises the following steps:

(1) the raw materials are respectively weighed according to the weight ratio.

(2) Pulverizing the above materials, decocting with 10-12 times of water for 2-3 times (each for 1-2 hr), filtering, mixing filtrates, and concentrating the filtrate under reduced pressure to obtain concentrated extract (1g is 3g of crude drug).

(3) Adding sucrose and dextrin (the ratio of sucrose to dextrin is 1: 1) into the concentrated extract obtained in the step 2, uniformly mixing, and preparing into granules (1g of granules is equivalent to 3g of crude drug).

Comparative example 2

A pharmaceutical composition of an astragalus root pulse-activating decoction, a preparation method and a feed are as follows according to parts by weight: 50 parts of astragalus, 30 parts of codonopsis pilosula, 25 parts of radix ophiopogonis, 15 parts of schisandra chinensis, 30 parts of echinacea, 25 parts of maca, 20 parts of vitamin C and 20 parts of citric acid.

The pharmaceutical composition, the preparation method and the preparation method of the feed of the astragalus root pulse-activating decoction are as follows:

(1) weighing the Chinese medicinal materials containing the above Chinese medicinal components, coarsely pulverizing, adding 10 parts of water, decocting for 2 times, each time for 1 hr, filtering, mixing filtrates, concentrating the filtrate under reduced pressure, drying to obtain dry extract, and pulverizing.

(2) And (3) respectively weighing vitamin C and citric acid, and uniformly mixing with the dry paste powder in the step (1) (1g of particles are equivalent to 3g of crude drug).

Comparative example 3

The astragalus pulse-activating decoction comprises the following components in parts by weight: 15 parts of astragalus, 9 parts of codonopsis pilosula, 9 parts of radix ophiopogonis and 5 parts of schisandra chinensis.

The preparation method of the radix astragali pulse-activating decoction comprises weighing the above medicines, pulverizing, adding 10 times of water, decocting for 2 times, each time for 1h, filtering, mixing filtrates, concentrating the filtrate under reduced pressure to obtain concentrated extract, adding dextrin, and making into granule (1g granule is equivalent to 3g crude drug).

Comparative example 4

50 parts of astragalus, 30 parts of codonopsis pilosula, 28 parts of radix ophiopogonis, 16 parts of schisandra chinensis, 15 parts of rock cowpea, 20 parts of Chinese fevervine herb and 18 parts of sweet wormwood.

The preparation method of the above comparative example 4 was:

(1) weighing the above traditional Chinese medicines, coarsely pulverizing, adding 10-12 times of water, decocting for 2-3 times, each time for 1-2 hours, filtering, mixing filtrates, and concentrating the filtrate under reduced pressure to obtain concentrated extract (1g is equivalent to 3g of crude drug) for use.

(2) Adding sucrose and dextrin (the ratio of sucrose to dextrin is 1: 1) into the concentrated extract obtained in the step 1, uniformly mixing, and preparing into granules (1g of granules is equivalent to 3g of crude drug).

In order to show the effects of the traditional Chinese medicine composition, the applicant further carries out clinical efficacy observation tests on the traditional Chinese medicine composition, and the beneficial effects of the traditional Chinese medicine composition are further illustrated by the following test examples.

Experimental example 1 test on the effect of the Chinese medicinal composition of the present invention on the antibody level of Newcastle disease vaccine of chicken

By observing the influence of the traditional Chinese medicine composition prepared in the embodiment of the invention on the antibody level of the chicken Newcastle disease vaccine, the immune enhancement effect of the traditional Chinese medicine composition is investigated.

1 Material

Test drugs: the traditional Chinese medicine composition prepared in the embodiments 1, 2, 3, 4 and 5 of the invention; the medicaments prepared in comparative examples 1, 2, 3, 4; control drugs: an Astragalus polysaccharides extract.

The test articles comprise NDV standard antigen and positive serum, and are used for determining the NDVHI antibody titer.

Test animals: hailan brown chicken

Test methods and groups: 240 helan brown chicks are randomly divided into 12 groups, 4 replicates are arranged in each group, 5 chicks are replicated in each group, the chicks are flatly raised on the net according to a conventional raising method, and the chicks are fed with complete formula feed without any medicine, are fed freely and are drunk with water. When the chicks are raised to 14 days old, ND vaccines are used for carrying out primary immunization through eye drop and nose drop; the second immunization was carried out by drinking water at the age of 28 days. After the first and second immunizations, the drugs were administered continuously for 7 days, with free water, at doses as given in table 1 below, 2 chickens were randomly selected from each replicate group at 21 and 35 days of age, and 8 chickens per group were collected intravenously at 0.2ml, and placed in 1.5ml of serum for centrifugal separation, and the ND antibody levels of the chickens were determined by HI method, and the specific grouping and treatment methods are as given in table 2 below.

TABLE 1 test grouping and dosing

2. Test results and analysis

TABLE 2 test results

As can be seen from table 2, the antibody levels in the example 5 group were the best elevated compared to the control drug, and were the optimal prescription for the present invention. Compared with comparative examples 1, 2 and 3, the 21 st and 35 th balance antibody levels after the first and second immunizations in the example 5 group are significantly improved, which shows that compared with the existing products with the same basic formula, the formula of the invention has significant effect. Compared with the comparative example 4, the 21 st balance antibody level of the group in the example 5 is increased from 4.25 to 5.56 after the first immunization, and the 35 th balance antibody level is increased from 6.73 to 7.67 after the second immunization, so that the addition of the mannooligosaccharides has obvious synergistic effect.

Experimental example 2 Observation of the preventive effect of the Chinese medicinal composition of the present invention on Newcastle disease

In 7 months of 2020, a chicken farm in Shandong with a history of Newcastle disease was selected for the test. 6000 avium broilers of 5 days old in the chicken farm were randomly divided into 4 groups of 1500, test 1, test 2, test 3 and test 4. The test 1 group and the test 2 group are the drug group of the invention, the test 3 group is the test group of the comparative example 1, and the test 1 group is added with 1 kg of the traditional Chinese medicine composition prepared in the embodiment 5 of the invention per ton of feed, is fed freely and is continuously administrated for 14 days; experiment 2 groups added 1 kg of the Chinese medicinal composition prepared in the invention example 1 per ton of feed, fed freely and administered for 14 days continuously; test 3 groups used the drug of comparative example 1, added 1 kg of the drug of comparative example 1 per ton of feed, fed freely and administered for 14 days continuously; test 4 group was a blank control group, and no drug was administered, and the feeding conditions were substantially identical. During the test, the work of isolation protection and disinfection is well done. After the administration, the observation was carried out for 40 days, and the test results are shown in Table 3.

TABLE 3 Newcastle disease prevention test results

Test results show that the traditional Chinese medicine composition prepared in the embodiment 5 and the embodiment 1 and the medicine in the comparative example 1 can effectively prevent the occurrence of the newcastle disease, the traditional Chinese medicine composition prepared in the embodiment 5 has the best effect, the morbidity is 0, the effective rate can reach 100 percent, and the traditional Chinese medicine composition is the optimal prescription of the invention, and the effect of preventing the newcastle disease of the invention is far better than that of the medicine group in the comparative example 1, and the difference of the traditional Chinese medicine composition and the medicine group is obvious compared with that of a blank control group. The medicine has obvious effect of preventing the newcastle disease.

Test example 3 Observation of the preventive Effect of the Chinese medicinal composition of the present invention on avian influenza

A chicken farm of Jiangsu Nanjing with a history of avian influenza is selected to carry out an avian influenza prevention test, and 2000 chicks are randomly divided into 4 groups of 500 chickens each. Group 1, 1 kg of the traditional Chinese medicine composition prepared in the embodiment 5 of the invention is added into each ton of feed for mixed feeding administration, and the feed is freely taken for 7 days; group 2, adding 1 kg of the traditional Chinese medicine composition prepared in the embodiment 3 of the invention into each ton of feed, and carrying out mixed feeding administration for 7 days after free feeding; group 3, 1 kg of the traditional Chinese medicine composition prepared in the comparative example 4 is added into each ton of feed for mixed feeding administration, and the feed is freely eaten for 7 days; in the first group, no drug was added to the feed. After the administration, the observation was carried out for 40 days. The test results are shown in Table 4.

TABLE 4 avian influenza prevention test results

Group of	Animal number (head)	Number of animals with disease (head)	Incidence of disease
				Group 1 (example 5)	500	0	0.00％
Group 2 (example 3)	500	1	0.200％
				Group 3 (comparative example 4)	500	25	5.00％
Group 4 (blank)	500	89	17.80％

Test results show that the traditional Chinese medicine composition of the embodiment 5 and the embodiment 3 and the medicine of the comparative example 4 have obvious effect on preventing avian influenza, the protection rates of the traditional Chinese medicine composition of the invention can reach 100 percent and 99.80 percent respectively, and are better than 95 percent of the comparative example 4, and the effect of synergy between the mannooligosaccharide and the traditional Chinese medicine composition of the invention is demonstrated. The test 1, 2 and 3 groups are significantly different from the blank control group. The Chinese medicinal composition has very good effect of preventing avian influenza.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.