阅读说明：本技术 一种淀粉基可降解包装材料 (Starch-based degradable packaging material ) 是由 崔波 赵焰 盛王民 于 2021-08-13 设计创作，主要内容包括：本发明提供了一种淀粉基可降解包装材料,属于包装材料技术领域。本发明提供的淀粉基包装材料为一种淀粉基保鲜膜,该保鲜膜能够有效的抑制革兰氏阴性菌和革兰氏阳性菌,并且可以有效的清除DPPH自由基,从而有效的抗氧化,因此,使用本发明提供的保鲜膜包装食物时,可以有效的抑制细菌和抗氧化,从而延长食物的保存时间。(The invention provides a starch-based degradable packaging material, and belongs to the technical field of packaging materials. The starch-based packaging material provided by the invention is a starch-based preservative film, which can effectively inhibit gram-negative bacteria and gram-positive bacteria and can effectively remove DPPH free radicals, so that the preservative film is effectively antioxidant, and therefore, when the preservative film provided by the invention is used for packaging food, bacteria and oxidation can be effectively inhibited, so that the storage time of the food is prolonged.)

1. The starch-based degradable preservative film is characterized by comprising the following preparation raw materials in percentage by mass: corn starch 5%, Ardisia japonica Makino polysaccharide 0.5-1.5%, glycerin 0.5%, sodium carboxymethylcellulose 0.5%, and water in balance.

2. The starch-based degradable preservative film according to claim 1, wherein the preparation method of the packaging material comprises the following steps:

(1) preparing 5% corn starch suspension, stirring at 70 deg.C, and gelatinizing for 30 min;

(2) adding 0.5% glycerol and 0.5% sodium hydroxymethyl cellulose, and stirring for 20 min;

(3) cooling to 40 ℃, adding 0.5-1.5% of flos lonicerae polysaccharide, and uniformly stirring to obtain a fresh-keeping solution;

(4) and adding the preservative solution into the mold, and drying to obtain the preservative film.

3. The starch-based degradable preservative film according to claim 1 or 2, wherein the preparation method of the Argentina malabarica polysaccharide comprises the following steps:

(1) cleaning flos Lonicerae, oven drying in a dryer, and soaking in 95% ethanol for 12 hr;

(2) taking out, drying in a dryer, and pulverizing into powder with a pulverizer;

(3) according to the following steps of 1: adding water in a material-liquid ratio of 20, uniformly stirring, and performing ultrasonic extraction for 2 hours at 500W ultrasonic power to obtain a flos lonicerae leaching solution;

(4) putting the flos Lonicerae extract into a centrifuge, centrifuging to remove precipitate, and concentrating the obtained supernatant under reduced pressure to 1/5 of the original volume to obtain crude polysaccharide extract of flos Lonicerae;

(5) adding a Sevage reagent with the volume of 1/4, fully oscillating, centrifuging, collecting supernatant, and repeating for 3 times to completely remove protein to obtain crude Ardisia japonica polysaccharide filtrate;

(6) adding 95% ethanol into the filtrate until the ethanol content reaches 80% to obtain crude Ardisia Maculosa polysaccharide precipitate, and washing the precipitate with anhydrous ethanol to obtain crude Ardisia Maculosa polysaccharide;

(7) adding water into the obtained crude Ardisia Maculosa polysaccharide to prepare 10mg/ml solution, filtering with 100KD ultrafiltration membrane, collecting filtrate, concentrating, and freeze drying to obtain Ardisia Maculosa polysaccharide.

4. The application of the Ardisia japonica Makino polysaccharide in preparing the antibacterial starch-based degradable preservative film is characterized in that the preparation method of the Ardisia japonica Makino polysaccharide comprises the following steps:

(1) cleaning flos Lonicerae, oven drying in a dryer, and soaking in 95% ethanol for 12 hr;

(2) taking out, drying in a dryer, and pulverizing into powder with a pulverizer;

(3) according to the following steps of 1: adding water in a material-liquid ratio of 20, uniformly stirring, and performing ultrasonic extraction for 2 hours at 500W ultrasonic power to obtain a flos lonicerae leaching solution;

(4) putting the flos Lonicerae extract into a centrifuge, centrifuging to remove precipitate, and concentrating the obtained supernatant under reduced pressure to 1/5 of the original volume to obtain crude polysaccharide extract of flos Lonicerae;

(5) adding a Sevage reagent with the volume of 1/4, fully oscillating, centrifuging, collecting supernatant, and repeating for 3 times to completely remove protein to obtain crude Ardisia japonica polysaccharide filtrate;

(6) adding 95% ethanol into the filtrate until the ethanol content reaches 80% to obtain crude Ardisia Maculosa polysaccharide precipitate, and washing the precipitate with anhydrous ethanol to obtain crude Ardisia Maculosa polysaccharide;

(7) adding water into the obtained crude Ardisia Maculosa polysaccharide to prepare 10mg/ml solution, filtering with 100KD ultrafiltration membrane, collecting filtrate, concentrating, and freeze drying to obtain Ardisia Maculosa polysaccharide.

5. Use according to claim 4, wherein the inhibition of bacteria comprises inhibition of gram-negative and gram-positive bacteria.

6. Use according to claim 5, wherein the gram-negative bacterium is E.coli and the gram-positive bacterium is S.aureus.

7. The use according to claim 4, wherein the preservative film is prepared by the following steps:

(1) preparing 5% corn starch suspension, stirring at 70 deg.C, and gelatinizing for 30 min;

(2) adding 0.5% glycerol and 0.5% sodium hydroxymethyl cellulose, and stirring for 20 min;

(3) cooling to 40 ℃, adding 0.5-1.5% of flos lonicerae polysaccharide, and uniformly stirring to obtain a fresh-keeping solution;

(4) and adding the preservative solution into the mold, and drying to obtain the preservative film.

8. The application of the Ardisia Mayiensis polysaccharide in preparing the antioxidant starch-based degradable preservative film is characterized in that the preparation method of the Ardisia Mayiensis polysaccharide comprises the following steps:

(1) cleaning flos Lonicerae, oven drying in a dryer, and soaking in 95% ethanol for 12 hr;

(2) taking out, drying in a dryer, and pulverizing into powder with a pulverizer;

(3) according to the following steps of 1: adding water in a material-liquid ratio of 20, uniformly stirring, and performing ultrasonic extraction for 2 hours at 500W ultrasonic power to obtain a flos lonicerae leaching solution;

(4) putting the flos Lonicerae extract into a centrifuge, centrifuging to remove precipitate, and concentrating the obtained supernatant under reduced pressure to 1/5 of the original volume to obtain crude polysaccharide extract of flos Lonicerae;

(5) adding a Sevage reagent with the volume of 1/4, fully oscillating, centrifuging, collecting supernatant, and repeating for 3 times to completely remove protein to obtain crude Ardisia japonica polysaccharide filtrate;

(6) adding 95% ethanol into the filtrate until the ethanol content reaches 80% to obtain crude Ardisia Maculosa polysaccharide precipitate, and washing the precipitate with anhydrous ethanol to obtain crude Ardisia Maculosa polysaccharide;

(7) adding water into the obtained crude Ardisia Maculosa polysaccharide to prepare 10mg/ml solution, filtering with 100KD ultrafiltration membrane, collecting filtrate, concentrating, and freeze drying to obtain Ardisia Maculosa polysaccharide.

9. The use according to claim 8, wherein the use comprises the use of the Ardisia japonica Makino polysaccharide in the preparation of starch-based degradable preservative films capable of scavenging DPPH radicals.

10. The use according to claim 8, wherein the preservative film is prepared by the following steps:

(1) preparing 5% corn starch suspension, stirring at 70 deg.C, and gelatinizing for 30 min;

(2) adding 0.5% glycerol and 0.5% sodium hydroxymethyl cellulose, and stirring for 20 min;

(3) cooling to 40 ℃, adding 0.5-1.5% of flos lonicerae polysaccharide, and uniformly stirring to obtain a fresh-keeping solution;

(4) and adding the preservative solution into the mold, and drying to obtain the preservative film.

Technical Field

The invention belongs to the technical field of packaging materials, and particularly relates to a starch-based degradable packaging material.

Background

At present, when food is packaged using a conventional packaging material, since the packaging material does not have antibacterial properties, it cannot effectively inhibit bacterial growth on the surface of the food, and thus the food is easily rotted when stored for a long period of time. Secondly, traditional packaging material is mostly the plastics material, can't be effectual degradation after the use, produces serious harm to natural environment and ecological balance easily.

With the improvement of environmental awareness of people, natural degradable food packaging gradually becomes a hot point of research, and the packaging material taking starch as a base material has wide sources and low price, so the packaging material has wide application prospects. However, the degradable packaging material prepared using starch has poor antibacterial properties, and thus it is necessary to add an antibacterial material thereto. At present, common food antibacterial agents are mostly chemically synthesized, and certain damage is generated to human bodies when the food antibacterial agents are used for a long time, so that a new natural antibacterial substance needs to be searched.

Disclosure of Invention

The invention aims to provide a bacteriostatic and antioxidant starch-based degradable packaging material.

In order to achieve the purpose, the invention provides the following technical scheme:

the invention provides a starch-based degradable preservative film, which comprises the following preparation raw materials in percentage by mass: corn starch 5%, Ardisia japonica Makino polysaccharide 0.5-1.5%, glycerin 0.5%, sodium carboxymethylcellulose 0.5%, and water in balance.

Preferably, the preparation method of the packaging material comprises the following steps:

(1) preparing 5% corn starch suspension, stirring at 70 deg.C, and gelatinizing for 30 min;

(2) adding 0.5% glycerol and 0.5% sodium hydroxymethyl cellulose, and stirring for 20 min;

(3) cooling to 40 ℃, adding 0.5-1.5% of flos lonicerae polysaccharide, and uniformly stirring to obtain a fresh-keeping solution;

(4) and adding the preservative solution into the mold, and drying to obtain the preservative film.

Preferably, the preparation method of the Ardisia argentea polysaccharide comprises the following steps:

(1) cleaning flos Lonicerae, oven drying in a dryer, and soaking in 95% ethanol for 12 hr;

(2) taking out, drying in a dryer, and pulverizing into powder with a pulverizer;

(3) according to the following steps of 1: adding water in a material-liquid ratio of 20, uniformly stirring, and performing ultrasonic extraction for 2 hours at 500W ultrasonic power to obtain a flos lonicerae leaching solution;

(4) putting the flos Lonicerae extract into a centrifuge, centrifuging to remove precipitate, and concentrating the obtained supernatant under reduced pressure to 1/5 of the original volume to obtain crude polysaccharide extract of flos Lonicerae;

(5) adding a Sevage reagent with the volume of 1/4, fully oscillating, centrifuging, collecting supernatant, and repeating for 3 times to completely remove protein to obtain crude Ardisia japonica polysaccharide filtrate;

(6) adding 95% ethanol into the filtrate until the ethanol content reaches 80% to obtain crude Ardisia Maculosa polysaccharide precipitate, and washing the precipitate with anhydrous ethanol to obtain crude Ardisia Maculosa polysaccharide;

(7) adding water into the obtained crude Ardisia Maculosa polysaccharide to prepare 10mg/ml solution, filtering with 100KD ultrafiltration membrane, collecting filtrate, concentrating, and freeze drying to obtain Ardisia Maculosa polysaccharide.

Secondly, the invention provides an application of the Ardisia Majorana Hance polysaccharide in preparing the antibacterial starch-based degradable preservative film, wherein the preparation method of the Ardisia Majorana Hance polysaccharide comprises the following steps:

(1) cleaning flos Lonicerae, oven drying in a dryer, and soaking in 95% ethanol for 12 hr;

(2) taking out, drying in a dryer, and pulverizing into powder with a pulverizer;

(3) according to the following steps of 1: adding water in a material-liquid ratio of 20, uniformly stirring, and performing ultrasonic extraction for 2 hours at 500W ultrasonic power to obtain a flos lonicerae leaching solution;

(4) putting the flos Lonicerae extract into a centrifuge, centrifuging to remove precipitate, and concentrating the obtained supernatant under reduced pressure to 1/5 of the original volume to obtain crude polysaccharide extract of flos Lonicerae;

(5) adding a Sevage reagent with the volume of 1/4, fully oscillating, centrifuging, collecting supernatant, and repeating for 3 times to completely remove protein to obtain crude Ardisia japonica polysaccharide filtrate;

(6) adding 95% ethanol into the filtrate until the ethanol content reaches 80% to obtain crude Ardisia Maculosa polysaccharide precipitate, and washing the precipitate with anhydrous ethanol to obtain crude Ardisia Maculosa polysaccharide;

(7) adding water into the obtained crude Ardisia Maculosa polysaccharide to prepare 10mg/ml solution, filtering with 100KD ultrafiltration membrane, collecting filtrate, concentrating, and freeze drying to obtain Ardisia Maculosa polysaccharide.

Preferably, the inhibiting includes inhibiting gram negative and gram positive bacteria.

Preferably, the gram-negative bacterium is escherichia coli and the gram-positive bacterium is staphylococcus aureus.

Preferably, the preparation steps of the preservative film are as follows:

(1) preparing 5% corn starch suspension, stirring at 70 deg.C, and gelatinizing for 30 min;

(2) adding 0.5% glycerol and 0.5% sodium hydroxymethyl cellulose, and stirring for 20 min;

(3) cooling to 40 ℃, adding 0.5-1.5% of flos lonicerae polysaccharide, and uniformly stirring to obtain a fresh-keeping solution;

(4) and adding the preservative solution into the mold, and drying to obtain the preservative film.

In addition, the invention provides application of the Ardisia japonica Makino polysaccharide in preparation of an antioxidant starch-based degradable preservative film, which is characterized in that the preparation method of the Ardisia japonica Makino polysaccharide comprises the following steps:

(1) cleaning flos Lonicerae, oven drying in a dryer, and soaking in 95% ethanol for 12 hr;

(2) taking out, drying in a dryer, and pulverizing into powder with a pulverizer;

(3) according to the following steps of 1: adding water in a material-liquid ratio of 20, uniformly stirring, and performing ultrasonic extraction for 2 hours at 500W ultrasonic power to obtain a flos lonicerae leaching solution;

(4) putting the flos Lonicerae extract into a centrifuge, centrifuging to remove precipitate, and concentrating the obtained supernatant under reduced pressure to 1/5 of the original volume to obtain crude polysaccharide extract of flos Lonicerae;

(5) adding a Sevage reagent with the volume of 1/4, fully oscillating, centrifuging, collecting supernatant, and repeating for 3 times to completely remove protein to obtain crude Ardisia japonica polysaccharide filtrate;

(6) adding 95% ethanol into the filtrate until the ethanol content reaches 80% to obtain crude Ardisia Maculosa polysaccharide precipitate, and washing the precipitate with anhydrous ethanol to obtain crude Ardisia Maculosa polysaccharide;

(7) adding water into the obtained crude Ardisia Maculosa polysaccharide to prepare 10mg/ml solution, filtering with 100KD ultrafiltration membrane, collecting filtrate, concentrating, and freeze drying to obtain Ardisia Maculosa polysaccharide.

Preferably, the application comprises the application of the lonicera malabarica polysaccharide in preparing the starch-based degradable preservative film capable of removing DPPH free radicals.

Preferably, the preparation steps of the preservative film are as follows:

(1) preparing 5% corn starch suspension, stirring at 70 deg.C, and gelatinizing for 30 min;

(2) adding 0.5% glycerol and 0.5% sodium hydroxymethyl cellulose, and stirring for 20 min;

(3) cooling to 40 ℃, adding 0.5-1.5% of flos lonicerae polysaccharide, and uniformly stirring to obtain a fresh-keeping solution;

(4) and adding the preservative solution into the mold, and drying to obtain the preservative film.

The invention has the beneficial effects that:

the starch-based degradable preservative film added with the Ardisia crenata polysaccharide can effectively inhibit gram-negative bacteria and gram-positive bacteria and can effectively clear DPPH free radicals, so that the preservative film has the functions of sterilization and oxidation resistance.

Drawings

FIG. 1 shows the inhibitory effect of wrap films A-D on E.coli;

FIG. 2 shows the inhibitory effect of preservative films A-D on Staphylococcus aureus;

FIG. 3 shows the effect of cling films A-D on DPPH radical scavenging.

Detailed Description

In order to clearly illustrate the technical features of the present solution, the present solution is explained below by way of specific embodiments.

Example 1

(1) Cleaning flos Lonicerae, oven drying in a dryer, and soaking in 95% ethanol for 12 hr;

(2) taking out, drying in a dryer, and pulverizing into powder with a pulverizer;

(3) adding 1000ml of the honeysuckle powder into 50g of the powder, uniformly stirring, and carrying out ultrasonic extraction for 2 hours at 500W of ultrasonic power to obtain a flos lonicerae leaching liquor;

(4) putting the flos Lonicerae extract into a centrifuge, centrifuging to remove precipitate, and concentrating the obtained supernatant under reduced pressure to 200ml to obtain flos Lonicerae crude polysaccharide extract;

(5) adding 50ml Sevage reagent, fully oscillating, centrifuging, collecting supernatant, repeating for 3 times to completely remove protein to obtain crude Ardisia crenata polysaccharide filtrate;

(6) adding 95% ethanol into the filtrate until the ethanol content reaches 80% to obtain crude Ardisia Maculosa polysaccharide precipitate, and washing the precipitate with anhydrous ethanol to obtain crude Ardisia Maculosa polysaccharide;

(7) adding water into the obtained crude Ardisia Maculosa polysaccharide to prepare 10mg/ml solution, filtering with 100KD ultrafiltration membrane, collecting filtrate, concentrating, and freeze drying to obtain Ardisia Maculosa polysaccharide.

Example 2

(1) Adding 5g of corn starch into 100ml of water, stirring and gelatinizing at 70 ℃ for 30 min;

(2) adding 0.5g of glycerol and 0.5g of sodium carboxymethylcellulose, stirring for 20min, and uniformly stirring to obtain a fresh-keeping solution;

(3) and adding the preservative solution into the mold, and drying to obtain the preservative film A.

Example 3

(1) Adding 5g of corn starch into 100ml of water, stirring and gelatinizing at 70 ℃ for 30 min;

(2) adding 0.5g of glycerol and 0.5g of sodium carboxymethylcellulose, and stirring for 20 min;

(3) cooling to 40 ℃, adding 0.5g of flos lonicerae polysaccharide, and uniformly stirring to obtain a fresh-keeping solution;

(4) and adding the preservative solution into the mold, and drying to obtain the preservative film B.

Example 4

(1) Adding 5g of corn starch into 100ml of water, stirring and gelatinizing at 70 ℃ for 30 min;

(2) adding 0.5g of glycerol and 0.5g of sodium carboxymethylcellulose, and stirring for 20 min;

(3) cooling to 40 ℃, adding 1g of flos lonicerae polysaccharide, and uniformly stirring to obtain a fresh-keeping solution;

(4) and adding the preservative solution into the mold, and drying to obtain the preservative film C.

Example 5

(1) Adding 5g of corn starch into 100ml of water, stirring and gelatinizing at 70 ℃ for 30 min;

(2) adding 0.5g of glycerol and 0.5g of sodium carboxymethylcellulose, and stirring for 20 min;

(3) cooling to 40 ℃, adding 1.5g of flos lonicerae polysaccharide, and uniformly stirring to obtain a fresh-keeping solution;

(4) and adding the preservative solution into the mold, and drying to obtain the preservative film D.

Example 6

(1) Diluting activated Escherichia coli or Staphylococcus aureus with sterile physiological saline to obtain 10⁶CFU/ml bacterial liquid;

(2) sterilizing the prepared beef extract peptone culture medium by high-pressure steam at 121 ℃, cooling to 50 ℃, pouring 30ml of the culture medium into a 90mm sterile flat plate, and solidifying for later use;

(3) uniformly coating 50ul of the bacterial liquid on a sterile flat plate;

(3) punching the preservative films A-D into disk films with the diameter of 8mm by using a puncher, and irradiating for 30min by using an ultraviolet lamp for sterilization;

(4) the membrane was clamped onto the medium using sterile clamps, with 3 replicates per group;

(5) and (3) placing the flat plate in an incubator, continuously culturing for 16h, and measuring the diameter of the inhibition zone after the culture is finished.

TABLE 1 detection of bacteriostatic effect of preservative film

Bacterial types	Plastic wrap A	Preservative film B	Plastic wrap C	Plastic wrap D
					Escherichia coli (mm)	0	10.67±0.94	14.18±0.71	16.09±0.58
Staphylococcus aureus (mm)	0	12.19±0.78	16.76±0.78	19.73±0.73

As can be seen from Table 1, the preservative film prepared by using only corn starch has no antibacterial effect, while the preservative film prepared by using the lonicera malabarica polysaccharide can effectively inhibit Escherichia coli, which is a gram-negative bacterium, and can effectively inhibit Staphylococcus aureus, which is a gram-positive bacterium, wherein the preservative film has a more excellent inhibitory effect on Staphylococcus aureus.

Example 7

(1) Preparation of 2X 10 with Anhydrous ethanol^-4Storing the DPPH solution in a brown bottle at low temperature for later use;

(2) punching the preservative films A-D into disk films with the diameter of 8mm by using a puncher;

(3) adding the preservative films A-D into DPPH solution (3 repeats are set for each group, no preservative film is added for a control group), and stirring for 30min in a dark place at room temperature;

(4) DPPH radical clearance = a₀-A₁/A₀×100%；

TABLE 2 DPPH radical scavenging Effect of cling films

From the results, the preservative film prepared by only using the corn starch has weaker antioxidant capacity, while the preservative film added with the lonicera malabarica polysaccharide can obviously reduce DPPH free radicals, which shows that the preservative film prepared by the lonicera malabarica polysaccharide can effectively remove DPPH free radicals, so that the preservative film has antioxidant effect.