阅读说明：本技术 一种白芷荧光快速检测技术 (Radix angelicae fluorescence rapid detection technology ) 是由 刘青 陈含笑 罗莎 张晓菊 王丹 位笑娜 黄继红 于 2021-08-03 设计创作，主要内容包括：本发明公开了一种白芷荧光快速检测技术,属于荧光检测技术领域,包括如下步骤：(1)母液制备；(2)全波长扫描；(3)选择三维荧光光谱测试条件；(4)三维荧光检测；(5)三维荧光图谱进行分析；(6)质量检测；(7)荧光检测方法的适用性的考察；(8)判定白芷的荧光检测样品浓度的限度；(9)考察放置时间对其荧光强度的影响。本申请是通过三维荧光方法建立一套快速、特异性强的白芷质量指纹图谱是提高中药材白芷质量的一种新方法。(The invention discloses a radix angelicae fluorescence rapid detection technology, belongs to the technical field of fluorescence detection, and comprises the following steps: (1) preparing mother liquor; (2) scanning at full wavelength; (3) selecting three-dimensional fluorescence spectrum test conditions; (4) three-dimensional fluorescence detection; (5) analyzing the three-dimensional fluorescence spectrum; (6) detecting the quality; (7) investigating the applicability of a fluorescence detection method; (8) judging the limit of the concentration of the fluorescence detection sample of the angelica dahurica; (9) the influence of the standing time on the fluorescence intensity was examined. The application is a new method for improving the quality of the Chinese medicinal material angelica dahurica by establishing a set of rapid and high-specificity angelica dahurica quality fingerprint by a three-dimensional fluorescence method.)

1. A radix angelicae fluorescence rapid detection technology is characterized by comprising the following steps:

(1) pulverizing radix Angelicae Dahuricae, sieving with 60 mesh sieve, accurately weighing 5g of dried radix Angelicae Dahuricae powder, adding 50mL of methanol, soaking at room temperature, standing for 3 hr, and vacuum filtering to obtain extract solution of 0.1g of radix Angelicae Dahuricae per milliliter as mother liquor;

(2) carrying out full-wavelength scanning on the extracting solution in the step (1), and then observing a scanning result;

(3) selecting a three-dimensional fluorescence spectrum test condition according to the full-wavelength scanning result of the step (2);

(4) detecting the radix angelicae extract by using three-dimensional fluorescence under the conditions in the step (3) to obtain a three-dimensional fluorescence spectrum;

(5) analyzing the three-dimensional fluorescence spectrum to find out a characteristic peak;

(6) after the characteristic peak sample is dissolved, measuring a two-dimensional fluorescence spectrum of the characteristic peak sample under the conditions of Ex320nm and Ex330nm, and substituting the fluorescence spectrum into the fluorescence spectrum of the radix angelicae formosanae, so that the quality detection of the radix angelicae formosanae can be completed;

(7) taking 1g of radix angelicae powder of four producing areas respectively, soaking the radix angelicae powder in 10mL of methanol for 3 hours, filtering, and carrying out fluorescence detection to investigate the applicability of a fluorescence detection method;

(8) preparing mother liquor of the methanol leaching solution of the angelica dahurica into different concentrations, and measuring fluorescence intensity at the Ex330 nm/Em 410nm to investigate the influence of the concentration on the fluorescence intensity so as to judge the limit of the concentration of the fluorescence detection sample of the angelica dahurica;

(9) preparing an angelica dahurica methanol extracting solution, placing the same mother solution into a 10mL centrifugal tube, sealing the mother solution by using a sealing film, carrying out fluorescence detection once every 3h under the conditions of Ex320nm, Ex330nm, Ex340nm and Ex350nm, wherein the labels are respectively N1, N2, N3, N4, N5 and N6, and examining the influence of the placing time on the fluorescence intensity of the mother solution.

2. The radix angelicae dahuricae fluorescence rapid detection technology according to claim 1, wherein the concentration of the mother liquor in step (1) is 0.1 g/mL.

3. The radix angelicae dahuricae fluorescence rapid detection technology according to claim 1, wherein the different concentrations in step (8) are 0.02, 0.04, 0.06, 0.08, 0.1g/mL, respectively.

Technical Field

The invention belongs to the technical field of fluorescence detection, and particularly relates to a radix angelicae fluorescence rapid detection technology.

Background

Because the traditional Chinese medicinal materials are generally used as crops for cultivation and production, the quality of the traditional Chinese medicinal materials is easily influenced by environmental factors and the like, and the quality is unstable. Therefore, quality control of traditional Chinese medicinal materials is always an important part of traditional Chinese medicine modernization, and is also an important threshold for Chinese medicinal materials to enter the military world. The angelica dahurica is one of 40 common bulk medicinal materials in China, has large yield and dosage, particularly wide production area distribution, and is cultivated in northeast, Shandong, Yunnan and other places except four large production places of the Hangzhou angelica dahurica, the Chuan angelica dahurica, the Qizhi and the Yuzhi. How to evaluate the quality of the angelica dahurica in different producing areas is particularly important.

At present, quality control of traditional Chinese medicinal materials is mainly standardized by pharmacopoeia, and the detection methods of radix angelicae in the latest pharmacopoeia in 2020 mainly comprise character identification, thin-layer chromatography identification and high performance liquid chromatography identification of extracts. The character identification method mainly depends on subjective judgment during identification, and an identifier is required to have rich traditional Chinese medicine identification experience, otherwise, the error rate or the error rate is high; the thin-layer chromatography cannot judge the content of components in a sample and can only determine the nature; the high performance liquid chromatography is a commonly used detection means with better qualitative and quantitative effects at present, but the detection time is longer. The quality control fingerprint of radix Angelicae Dahuricae can be detected by ultraviolet and infrared detection. However, the ultraviolet specificity is poor, the infrared spectrum is too complex, and the judgment is not easy to carry out.

Disclosure of Invention

The invention aims to provide a radix angelicae fluorescence rapid detection technology aiming at the existing problems.

The invention is realized by the following technical scheme:

a radix angelicae fluorescence rapid detection technology comprises the following steps:

(1) pulverizing radix Angelicae Dahuricae, sieving with 60 mesh sieve, accurately weighing 5g of dried radix Angelicae Dahuricae powder, adding 50mL of methanol, soaking at room temperature, standing for 3 hr, and vacuum filtering to obtain extract solution of 0.1g of radix Angelicae Dahuricae per milliliter as mother liquor;

(2) carrying out full-wavelength scanning on the extracting solution in the step (1), and then observing a scanning result;

(3) selecting a three-dimensional fluorescence spectrum test condition according to the full-wavelength scanning result of the step (2);

(4) detecting the radix angelicae extract by using three-dimensional fluorescence under the conditions in the step (3) to obtain a three-dimensional fluorescence spectrum;

(5) analyzing the three-dimensional fluorescence spectrum to find out a characteristic peak;

(6) after the characteristic peak sample is dissolved, measuring a two-dimensional fluorescence spectrum of the characteristic peak sample under the conditions of Ex320nm and Ex330nm, and substituting the fluorescence spectrum into the fluorescence spectrum of the radix angelicae formosanae, so that the quality detection of the radix angelicae formosanae can be completed;

(7) taking 1g of radix angelicae powder of four producing areas respectively, soaking the radix angelicae powder in 10mL of methanol for 3 hours, filtering, and carrying out fluorescence detection to investigate the applicability of a fluorescence detection method;

(8) preparing mother liquor of the methanol leaching solution of the angelica dahurica into different concentrations, and measuring fluorescence intensity at the Ex330 nm/Em 410nm to investigate the influence of the concentration on the fluorescence intensity so as to judge the limit of the concentration of the fluorescence detection sample of the angelica dahurica;

(9) preparing an angelica dahurica methanol extracting solution, placing the same mother solution into a 10mL centrifugal tube, sealing the mother solution by using a sealing film, carrying out fluorescence detection once every 3h under the conditions of Ex320nm, Ex330nm, Ex340nm and Ex350nm, wherein the labels are respectively N1, N2, N3, N4, N5 and N6, and examining the influence of the placing time on the fluorescence intensity of the mother solution.

Further, the method is carried out. The concentration of the mother liquor in the step (1) is 0.1 g/mL.

Further, the different concentrations in step (8) are 0.02, 0.04, 0.06, 0.08 and 0.1g/mL respectively.

Compared with the prior art, the invention has the following advantages:

1. the characteristic that the main effective component in the angelica dahurica is the coumarin compound is used, and the coumarin compound has a larger conjugated system and is a kind with strong fluorescence in nature. Therefore, the method has low detection limit, and can achieve the detection result only by a small amount of samples.

2. The fluorescence method has specific absorption wavelength and emission wavelength for different compounds, so the selectivity is strong, for example, the specific detection condition of angelica dahurica is the range of excitation wavelength (Ex): 300-330 nm, sampling interval 10nm and Ex Slit 5 nm; the emission wavelength (Em) range is 375-650 nm, the sampling interval is 5nm, and the Em Slit is 5 nm; integration time 0.1 s.

3. The fluorescence method is generally not affected by external factors, such as temperature, humidity, solvent and the like, and is simple and convenient for operators to use.

4. The fluorescence method has no loss to the sample, the standard substance or the detection sample can be recovered, and a large amount of organic solvent waste liquid can not be generated like other detection methods.

5. The fluorescence method has high detection speed, a fluorescence detection map can be obtained within 1 minute, and other detection methods require long time, such as thin layer chromatography for about 15 minutes and high performance liquid chromatography for about 1 hour.

6. A rapid and specific radix angelicae dahuricae quality fingerprint spectrum is established by a three-dimensional fluorescence method, which is a new method for improving the quality of the traditional Chinese medicine radix angelicae dahuricae.

Drawings

FIG. 1 is a fluorescence spectrum of the radix Angelicae Dahuricae extractive solution at multiple wavelengths according to the present application;

FIG. 2 is a three-dimensional fluorescence spectrum of the radix Angelicae Dahuricae extractive solution under the three-dimensional fluorescence spectrum test condition of the present application;

FIG. 3 is a imperatorin fluorescence spectrum of the present application;

FIG. 4 is a fluorescent spectrum of isophellopterin according to the present application;

fig. 5 is a three-dimensional fluorescence spectrum of radix angelicae formosanae of the present application;

FIG. 6 is a three-dimensional fluorescence spectrum of Chuan Dahurian Angelica root of the present application;

fig. 7 is a three-dimensional fluorescence spectrum of dahurian angelica root according to the present application;

FIG. 8 is a three-dimensional fluorescence spectrum of Angelica keiskei according to the present application;

FIG. 9 shows fluorescence spectra of Angelica dahurica body fluid with different concentrations according to the present application;

FIG. 10 shows the results of fluorescence for six time periods for four emissions of the present application.

Detailed Description

A radix angelicae fluorescence rapid detection technology comprises the following steps:

(1) pulverizing radix Angelicae Dahuricae, radix Angelicae Dahuricae and radix Angelicae Pubescentis respectively, sieving with 60 mesh sieve, accurately weighing 5g dried radix Angelicae Dahuricae powder, adding 50mL methanol, soaking at room temperature, standing for 3 hr, and vacuum filtering to obtain 0.1g plant radix Angelicae Dahuricae extractive solution as mother liquor (0.1 g/mL);

(2) carrying out full-wavelength scanning on the extracting solution, wherein two fluorescence peaks in the range of Ex 300-330 nm can be seen according to the scanning result, the appearance of the peaks is obvious, and when the excitation wavelength is increased, the fluorescence peak with the emission wavelength near 500nm becomes not obvious any more (see figure 1);

(3) the selected three-dimensional fluorescence spectrum testing conditions are as follows: excitation wavelength (Ex) range: 300-330 nm, sampling interval 10nm and Ex Slit 5 nm; the emission wavelength (Em) range is 375-650 nm, the sampling interval is 5nm, and the Em Slit is 5 nm; integration time 0.1 s;

(4) detecting radix Angelicae Dahuricae extractive solution with three-dimensional fluorescence under the above conditions to obtain the following characteristic map (see FIG. 2);

(5) analyzing two characteristic peaks on the three-dimensional map, and finding characteristic absorption peaks of imperatorin and isophellopterin in the angelica dahurica respectively;

(6) dissolving 10mg of imperatorin sample in methanol, placing the solution in a 50mL volumetric flask, and fixing the volume to a scale to obtain 0.2mg/mL imperatorin solution; dissolving 88.9mg of isophellopterin with a small amount of methanol to obtain a constant volume of 100mL, and accurately transferring 2.3mL of the prepared isophellopterin solution into a 10mL volumetric flask by using a liquid transfer gun to obtain an isophellopterin solution with a concentration of 2.04 mg/mL. Under the conditions of Ex320nm and Ex330nm, two-dimensional fluorescence spectra of imperatorin and isophellopterin are respectively shown in figure 3 and figure 4;

(7) substituting the fluorescent spectrums of the two coumarins into the fluorescent spectrum of the radix angelicae dahuricae extracting solution to find that the peak emergence positions of the fluorescent spectrums of the two coumarins are basically consistent with the peak emergence position of the radix angelicae dahuricae, which shows that the fluorescent peak at the position of radix angelicae Ex330 nm/Em 410nm contains isophellandrin, and the fluorescent peak at the position of Ex320 nm/Em 490nm contains imperatorin;

(8) imperatorin and isophellopterin are coumarin compounds with larger content in radix angelicae, wherein the content of imperatorin is the quality standard of radix angelicae evaluation specified in 2020 edition Chinese pharmacopoeia;

(9) the main effective component in the angelica dahurica is furocoumarin compound which can generate fluorescence under the irradiation of ultraviolet light, so that the stronger the fluorescence is, the higher the content of the effective component in the angelica dahurica is, namely the better the drug effect is;

(10) taking 1g of radix angelicae dahuricae powder from four production places respectively, soaking the powder in 10mL of methanol for 3h, filtering, and performing fluorescence detection, wherein the method is suitable for quality detection of radix angelicae dahuricae from different production places, and the steps are as follows from the sequence shown in FIGS. 5-8: radix Angelicae Dahuricae, and radix Angelicae Dahuricae;

(11) the newly prepared methanol extract mother liquor of radix angelicae dahuricae is prepared into different concentrations (0.02, 0.04, 0.06, 0.08, 0.1g/mL), the fluorescence intensity is measured at the Ex330 nm/Em 410nm position to examine the influence of the concentrations on the fluorescence intensity, and the two-dimensional fluorescence spectrum of the methanol extract of radix angelicae dahuricae with different concentrations is shown in FIG. 9;

(12) the detection result shows that the fluorescence intensity of the test solution of 0.08g/mL and 0.1g/mL is basically the same, which indicates that the fluorescence of the radix angelicae test solution is quenched under the concentration gradient, so that the higher the concentration is, the lower the fluorescence intensity is;

(13) the concentration limit of the fluorescence detection sample of the angelica dahurica is 0.02-0.08 g/mL;

(14) preparing the methanol extract of the angelica dahurica, and investigating the influence of the standing time on the fluorescence intensity of the extract. Putting the same mother solution into a 10mL centrifuge tube, sealing the centrifuge tube by using a sealing film, and carrying out fluorescence detection once every 3h under the conditions of Ex320nm, Ex330nm, Ex340nm and Ex350nm, wherein the labels are respectively N1, N2, N3, N4, N5 and N6, and the fluorescence spectrum is shown in the figure 10;

(15) it can be seen from the figure that the detection results of six time points are basically coincident under different fluorescence intensities, which proves that the method has extremely small change under the influence of time factors.

The fluorescence method has specific absorption wavelength and emission wavelength for different compounds, so the selectivity is strong, for example, the specific detection condition of angelica dahurica is the range of excitation wavelength (Ex): 300-330 nm, sampling interval 10nm and Ex Slit 5 nm; the emission wavelength (Em) range is 375-650 nm, the sampling interval is 5nm, and the Em Slit is 5 nm; integration time 0.1 s; the method can highlight important active ingredients in radix Angelicae Dahuricae, such as imperatorin and isophellopterin; the concentration limit of the fluorescence detection sample of the angelica dahurica sample should be 0.02-0.08 g/mL.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the present invention is not limited to the illustrated embodiments, and all the modifications and equivalents of the embodiments may be made without departing from the spirit of the present invention.