阅读说明：本技术 浒苔多世代连续培养装置及其培养方法 (Enteromorpha prolifera multi-generation continuous culture device and culture method thereof ) 是由 王影 侯承宗 唐学玺 曲同飞 赵新宇 许金慧 钟怡 管晨 林志豪 孙白雪 于 2021-09-10 设计创作，主要内容包括：本发明提供了一种浒苔多世代连续培养装置及其培养方法,涉及浒苔扩培技术领域。所述浒苔多世代连续培养装置包括光照培养单元、底照培养单元和抽滤单元；其中,所述抽滤单元包括具有滤网的水泵,可以将光照培养单元中的浒苔孢子或配子抽滤至底照培养单元中；所述浒苔孢子或配子附着于底照培养单元的附着基质上进行生长,得到多世代浒苔。通过本申请上述培养装置有效实现了浒苔的多世代连续培养,缓解了现有培养箱单一培养浒苔所存在的样本不稳定,以及难以满足科研人员对浒苔孢子体,配子体萌发过程及特定生活史阶段的实验及观测需求的问题。(The invention provides an enteromorpha prolifera multi-generation continuous culture device and a culture method thereof, and relates to the technical field of enteromorpha prolifera expanding culture. The enteromorpha multi-generation continuous culture device comprises an illumination culture unit, a bottom illumination culture unit and a suction filtration unit; the suction filtration unit comprises a water pump with a filter screen, and can suction filter enteromorpha spores or gametes in the illumination culture unit into the base illumination culture unit; and the enteromorpha spores or gametes are attached to the attachment matrix of the substrate culture unit for growth, so that the multi-generation enteromorpha is obtained. Through the above-mentioned culture apparatus of this application effectively realized the many generations continuous culture of waterside tongue, it is unstable to have alleviated the sample that current incubator list one was cultivateed the waterside tongue and exists to and be difficult to satisfy the scientific research personnel to the experiment and the problem of observing the demand of waterside tongue sporophyte, gametophyte germination process and specific life history stage.)

1. The multi-generation continuous enteromorpha culture device is characterized by comprising a light culture unit (1), a bottom light culture unit (2) and a suction filtration unit (3);

the suction filtration unit (3) comprises a water pump with a filter screen (31) and is used for suction filtration of enteromorpha spores or gametes in the illumination culture unit (1) into the base illumination culture unit (2);

the base illumination culture unit (2) comprises an attachment matrix (21) for attachment of enteromorpha spores or gametes.

2. The multi-generation continuous enteromorpha culture device according to claim 1, wherein the under-illumination culture unit (2) further comprises an under-illumination culture box (22) and an under-illumination light source (23).

3. The multi-generation continuous enteromorpha cultivation device according to claim 1, wherein the illumination cultivation unit (1) comprises an illumination incubator (11) and an intense light source (12).

4. The multi-generation continuous enteromorpha cultivation device according to claim 1, wherein the filter screen (31) in the water pump with the filter screen (31) is a filter screen (31) which is 900-1600 meshes, preferably a filter screen (31) which is 1600 meshes.

5. The multi-generation continuous enteromorpha culture device according to claim 1, further comprising a liquid feeding unit (4) and a liquid discharging unit (5);

preferably, the liquid feeding unit (4) comprises an artificial seawater bottle, and the liquid feeding unit (4) is communicated with the illumination culture unit (1) through a pipeline;

preferably, the drainage unit (5) comprises a waste liquid tank, and the drainage unit (5) is in pipeline communication with the bottom illumination culture unit (2).

6. The enteromorpha multi-generation continuous culture device according to claim 5, wherein peristaltic pumps are arranged on the pipelines of the liquid feeding unit (4) and the illumination culture unit (1);

preferably, peristaltic pumps are arranged on the pipelines of the liquid discharge unit (5) and the bottom illumination culture unit (2).

7. The multi-generation continuous enteromorpha culture device according to claim 1, further comprising a carbon dioxide generator (6), wherein the carbon dioxide generator (6) is respectively communicated with the liquid feeding unit (4) and the illumination culture unit (1) through pipelines.

8. The multi-generation continuous culture method of enteromorpha is characterized by comprising the following steps:

(a) selecting a whole plant of the enteromorpha prolifera which is fixed as a mother plant and placing the mother plant in the illumination culture unit (1) for culture; then, a culture solution containing enteromorpha spores or gametes in the illumination culture unit (1) is pumped and filtered into the base illumination culture unit (2) by using a pumping and filtering unit (3);

(b) leaching the enteromorpha spores or gametes in the step (a) until the enteromorpha spores or gametes in the base illumination culture unit (2) are attached to an attachment matrix (21) of the base illumination culture unit (2) for growth to obtain enteromorpha plants;

(c) and (c) separating the enteromorpha plants obtained by the culture in the step (b) from the attachment matrix (21), and then placing the enteromorpha plants in a light culture unit (1) for culture to obtain the enteromorpha cultured in a multi-generation continuous manner.

9. The multi-generation continuous cultivation method of enteromorpha according to claim 8, wherein the components of the culture solution in the illumination culture unit (1) and/or the bottom illumination culture unit (2) comprise: 0.1203mg/L nitrate, 0.043mg/L ammonia salt and 0.0135mg/L phosphate;

preferably, the salinity of the culture solution is 27-32%, preferably 30%.

10. The multi-generation continuous cultivation method of enteromorpha according to claim 8, wherein the light culture sheet of step (a)The illumination intensity of the element (1) is 100 to 150 [ mu ] mol/m²s, at the temperature of 15-22 ℃;

preferably, the light intensity of the light culture unit (1) in the step (a) is 120 [ mu ] mol/m²s, temperature 20 ℃;

preferably, the illumination intensity of the bottom illumination culture unit (2) in the step (b) is 70-80 mu mol/m²s, at the temperature of 15-22 ℃;

more preferably, the illumination intensity of the step (b) bottom illumination culture unit (2) is 72 mu mol/m²s, temperature 20 ℃.

Technical Field

The invention relates to the technical field of enteromorpha expanding culture, in particular to an enteromorpha multi-generation continuous culture device and a culture method thereof.

Background

At present, most of enteromorpha algae are cultured in a single quick and continuous culture box in a laboratory, and the aim is to realize the amplification of enteromorpha biomass in a short time.

However, the disadvantage of single culture in an incubator is that: and (I) although a large amount of enteromorpha fronds are obtained in a short period, the experimental and observation requirements of scientific researchers on the germination process and the gametophyte germination process of the enteromorpha sporophytes and the gametophyte and a specific life history stage are difficult to meet. Secondly, vegetative reproduction is usually carried out by biomass amplification with algae segments, and the activity and survival time of the vegetative reproduction are mainly determined by the activity state of the mother plant. In addition, the algae body amplified by the algae section is usually in a floating state, the activity of the algae body is possibly inferior to that of the enteromorpha which is directly grown and developed by fixed spores or gametes, and finally, an enteromorpha sample in a laboratory is difficult to stably culture, and the phenomenon of whitening or death occurs, so that the experiment burden of scientific researchers is further increased.

In view of the above, a method for obtaining samples of enteromorpha in each stage of life history in a laboratory is researched and constructed, so that the enteromorpha can be stably cultured in multiple generations, the problem that experimental samples are insufficient for long-term simulation observation of enteromorpha in the laboratory of existing scientific researchers is solved, and good samples are provided for researching changes of important life history processes of enteromorpha and response differences of each stage of life history of enteromorpha to changes and stresses of a plurality of typical environmental factors, and the method becomes necessary and urgent.

In view of the above, the present invention is particularly proposed.

Disclosure of Invention

The invention provides a multi-generation continuous enteromorpha culture device, which effectively realizes multi-generation continuous culture of enteromorpha and solves the problems that a sample for singly culturing enteromorpha in the conventional culture box is unstable and the experimental and observation requirements of scientific researchers on the enteromorpha sporophyte and gametophyte germination process and a specific life history stage are difficult to meet.

The second purpose of the invention is to provide a multi-generation continuous culture method of enteromorpha, which effectively realizes multi-generation continuous culture of enteromorpha by utilizing the multi-generation continuous culture device of enteromorpha.

The invention provides a multi-generation continuous enteromorpha culture device which comprises an illumination culture unit, a bottom illumination culture unit and a suction filtration unit;

the suction filtration unit comprises a water pump with a filter screen and is used for suction filtration of enteromorpha spores or gametes in the illumination culture unit into the base illumination culture unit;

the base illumination culture unit comprises an attachment substrate for attachment of enteromorpha spores or gametes.

Further, the bottom lighting culture unit also comprises a bottom lighting culture box and a bottom lighting light source.

Further, the illumination culture unit comprises an illumination culture box and an intense light source.

Furthermore, the filter screen in the water pump with the filter screen is 900-1600 meshes of filter screen, and is preferably 1600 meshes of filter screen.

Further, the culture device also comprises a liquid feeding unit and a liquid discharging unit;

preferably, the liquid feeding unit comprises an artificial seawater bottle, and is communicated with the illumination culture unit through a pipeline;

preferably, the drainage unit comprises a waste liquid tank, and the drainage unit is in pipeline communication with the bottom illumination culture unit.

Furthermore, peristaltic pumps are arranged on the pipelines of the liquid feeding unit and the illumination culture unit;

preferably, peristaltic pumps are arranged on the pipelines of the liquid discharge unit and the bottom illumination culture unit.

Further, the culture device also comprises a carbon dioxide generator which is respectively communicated with the liquid feeding unit and the illumination culture unit through pipelines.

The invention provides a multi-generation continuous culture method of enteromorpha, which comprises the following steps:

(a) selecting a whole plant of the Enteromorpha prolifera which is fixed as a mother plant and placing the mother plant in an illumination culture unit for culture; then, a culture solution containing enteromorpha spores or gametes in the illumination culture unit is pumped and filtered into the base illumination culture unit by using a pumping and filtering unit;

(b) leaching the enteromorpha spores or gametes in the base illumination culture unit to be attached to the attachment matrix of the base illumination culture unit for growth to obtain enteromorpha plants;

(c) and (c) separating the enteromorpha plants obtained by the culture in the step (b) from the attachment matrix, and then placing the enteromorpha plants in a light culture unit for culture to obtain the enteromorpha cultured continuously for multiple generations.

Further, the culture solution in the illumination culture unit and/or the bottom illumination culture unit comprises the following components: 0.1203mg/L nitrate, 0.043mg/L ammonia salt and 0.0135mg/L phosphate;

preferably, the salinity of the culture solution is 27-32 per mill, and preferably 30 per mill.

Further, the illumination intensity of the illumination culture unit in the step (a) is 100-150 mu mol/m²s, at the temperature of 15-22 ℃;

preferably, the light intensity of the light culture unit of the step (a) is 120. mu. mol/m²s, temperature 20 ℃;

preferably, the illumination intensity of the bottom illumination culture unit in the step (b) is 70-80 mu mol/m²s, at the temperature of 15-22 ℃;

more preferably, the light intensity of the step (b) bottom illumination culture unit is 72 μmol/m²s, temperature 20 ℃.

Compared with the prior art, the invention has the beneficial effects that:

the invention provides an enteromorpha multi-generation continuous culture device which comprises a light culture unit, a bottom light culture unit and a suction filtration unit; the suction filtration unit comprises a water pump with a filter screen, and can suction filter enteromorpha spores or gametes in the illumination culture unit into the base illumination culture unit; and the enteromorpha spores or gametes are attached to the attachment matrix of the substrate culture unit for growth, so that the multi-generation enteromorpha is obtained. Through the above-mentioned culture apparatus of this application effectively realized the many generations continuous culture of waterside tongue, it is unstable to have alleviated the sample that current incubator list one was cultivateed the waterside tongue and exists to and be difficult to satisfy the scientific research personnel to the experiment and the problem of observing the demand of waterside tongue sporophyte, gametophyte germination process and specific life history stage.

According to the multi-generation continuous culture method of enteromorpha provided by the invention, firstly, a whole plant of the enteromorpha which is fixedly grown is selected as a mother plant and is placed in an illumination culture unit for culture; then, a culture solution containing enteromorpha spores or gametes in the illumination culture unit is pumped and filtered into the base illumination culture unit by using a pumping and filtering unit; then, the enteromorpha spores or gametes are attached to the attachment matrix of the base illumination culture unit for growth to obtain enteromorpha plants; and separating the enteromorpha plants from the attachment matrix, and culturing in an illumination culture unit to obtain the enteromorpha cultured in a multi-generation continuous manner. According to the method, the multi-generation continuous culture of the enteromorpha is effectively realized by using the multi-generation continuous culture device of the enteromorpha, and the problems that a sample is unstable and observation of each living section is not facilitated when the enteromorpha is cultured in a single culture box in the prior art are solved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

Fig. 1 is a schematic structural diagram of an enteromorpha multi-generation continuous culture device provided in embodiment 1 of the present invention;

FIG. 2 is a schematic structural diagram of a multi-generation continuous cultivation apparatus for Enteromorpha prolifera including a water supply and drainage unit according to example 1 of the present invention;

FIG. 3 is a diagram of a spore and male and female gametes of example 1 of the present invention swimming onto the bottom plate and fixing;

FIG. 4 is a diagram of an Enteromorpha prolifera in an illumination incubator after seedlings are peeled off a plate according to example 1 of the present invention;

FIG. 5 is a diagram of an Enteromorpha prolifera entity obtained by continuously culturing Enteromorpha prolifera cultured in an illumination incubator for 7-14 days, which is provided by embodiment 1 of the invention.

Icon: 1-light culture unit; 2-a bottom illumination culture unit; 3-a suction filtration unit; 31-a filter screen; 21-an attachment matrix; 22-bottom illumination incubator; 23-a bottom illumination source; 11-light incubator; 12-an intense light source; 4-a liquid feeding unit; 5-a liquid discharge unit; 6-carbon dioxide generator.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

According to one aspect of the invention, the enteromorpha multi-generation continuous culture device comprises a light culture unit 1, a bottom light culture unit 2 and a suction filtration unit 3;

the suction filtration unit 3 comprises a water pump with a filter screen 31 and is used for suction filtration of enteromorpha spores or gametes in the illumination culture unit 1 into the base illumination culture unit 2;

the base illumination culture unit 2 comprises an attachment matrix 21 for attachment of enteromorpha spores or gametes.

The invention provides a multi-generation continuous enteromorpha culture device, which comprises an illumination culture unit 1, a bottom illumination culture unit 2 and a suction filtration unit 3; the suction filtration unit 3 comprises a water pump with a filter screen 31, and can suction filter enteromorpha spores or gametes in the illumination culture unit 1 into the base illumination culture unit 2; and the enteromorpha spores or gametes are attached to the attachment matrix 21 of the base illumination culture unit 2 for growth, so that the multi-generation enteromorpha is obtained. Through the above-mentioned culture apparatus of this application effectively realized the many generations continuous culture of waterside tongue, it is unstable to have alleviated the sample that current incubator list one was cultivateed the waterside tongue and exists to and be difficult to satisfy the scientific research personnel to the experiment and the problem of observing the demand of waterside tongue sporophyte, gametophyte germination process and specific life history stage.

In a preferred embodiment of the present invention, the underillumination incubation unit 2 further comprises an underillumination incubator 22 and an underillumination light source 23.

Preferably, the bottom illumination incubator 22 is covered with black shading cloth at the top and a disposable plate is placed at the bottom as the attachment substrate 21, and light transmission from the bottom of the cylinder to the upper light is induced only by the bottom illumination light source 23.

In a preferred embodiment of the present invention, the light culturing unit 1 comprises a light culturing chamber 11 and an intense light source 12.

Preferably, the illumination incubator 11 utilizes the strong light source 12 to perform strong light irradiation stimulation on the enteromorpha to induce the generation of enteromorpha germ cells.

In a preferred embodiment of the present invention, the screen 31 of the water pump with the screen 31 is a screen 31 passing through 900-1600 meshes, and preferably a screen 31 passing through 1600 meshes.

In a preferred embodiment, the water pump with the filter screen 31 can ensure that the microscopic propagules filtered by the bottom illumination incubator 22 are basically spores and male and female gametes released by enteromorpha plants.

In a preferred embodiment of the present invention, the culture apparatus further comprises a liquid feeding unit 4 and a liquid discharging unit 5;

in the above preferred embodiment, the liquid feeding unit 4 comprises an artificial seawater bottle, and the liquid feeding unit 4 is in pipeline communication with the light culture unit 1;

in the preferred embodiment described above, the drainage unit 5 comprises a waste liquid tank, and the drainage unit 5 is in pipe communication with the bottom-lighting culture unit 2.

As a preferred embodiment, the liquid feeding unit 4 and the liquid discharging unit 5 are used for liquid conveying and discharging of the light irradiation culture unit 1 and the bottom irradiation culture unit 2, and the stability of the enteromorpha culture environment in the culture process is ensured by a water changing method.

Preferably, the culture solution in the artificial seawater bottle has the same composition as the culture solution in the light irradiation culture unit 1 and the bottom irradiation culture unit 2.

In the above preferred embodiment, peristaltic pumps are arranged on the pipelines of the liquid feeding unit 4 and the light irradiation culture unit 1;

preferably, peristaltic pumps are arranged on the pipelines of the liquid discharge unit 5 and the bottom illumination culture unit 2.

In a preferred embodiment of the present invention, the culture apparatus further comprises a carbon dioxide generator 6, and the carbon dioxide generator 6 is in pipe communication with the liquid feeding unit 4 and the light irradiation culture unit 1, respectively.

As a preferred embodiment, the carbon dioxide generator 6 can provide more carbon sources for enteromorpha, and is helpful for the growth and development of enteromorpha.

According to one aspect of the present invention, a multi-generation continuous culture method of enteromorpha comprises the following steps:

(a) selecting a whole plant of the Enteromorpha prolifera which is fixed as a mother plant and placing the mother plant in the illumination culture unit 1 for culture; then, a culture solution containing enteromorpha spores or gametes in the illumination culture unit 1 is pumped and filtered into the base illumination culture unit 2 by using a pumping and filtering unit 3;

(b) leaching the enteromorpha spores or gametes in the base illumination culture unit 2 to adhere to the adhesion matrix 21 of the base illumination culture unit 2 for growth to obtain enteromorpha plants;

(c) separating the enteromorpha plants obtained by the culture in the step (b) from the attachment matrix 21, and then placing the enteromorpha plants in the illumination culture unit 1 for culture to obtain the enteromorpha cultured in a multi-generation continuous way.

According to the multi-generation continuous culture method of enteromorpha provided by the invention, firstly, a whole plant of the enteromorpha which is fixedly grown is selected as a mother plant and is placed in an illumination culture unit 1 for culture; then, a culture solution containing enteromorpha spores or gametes in the illumination culture unit 1 is pumped and filtered into the base illumination culture unit 2 by using a pumping and filtering unit 3; then, enteromorpha spores or gametes are attached to the attachment matrix 21 of the base illumination culture unit 2 to grow, and enteromorpha plants are obtained; and separating the enteromorpha plants from the attachment matrix 21, and culturing the enteromorpha plants in the illumination culture unit 1 to obtain the enteromorpha which is continuously cultured for multiple generations. According to the method, the multi-generation continuous culture of the enteromorpha is effectively realized by using the multi-generation continuous culture device of the enteromorpha, and the problems that a sample is unstable and observation of each living section is not facilitated when the enteromorpha is cultured in a single culture box in the prior art are solved.

In a preferred embodiment of the present invention, the culture solution in the light culture unit 1 and/or the bottom light culture unit 2 comprises: 0.1203mg/L nitrate, 0.043mg/L ammonia salt and 0.0135mg/L phosphate;

preferably, the salinity of the culture solution is 27-32 per mill, and preferably 30 per mill.

In a preferred embodiment of the present invention, the light intensity of the light culturing unit 1 in the step (a) is 100 to 150. mu. mol/m²s, at the temperature of 15-22 ℃;

in the above preferred embodiment, the light intensity of the light culturing unit 1 of the step (a) is 120. mu. mol/m²s, temperature 20 ℃; in a preferred embodiment, the light irradiation culture unit 1 induces the enteromorpha to produce spores or gametes by strong light, thereby realizing generation continuity.

In a preferred embodiment of the present invention, the illumination intensity of the bottom illumination culture unit 2 in the step (b) is 70 to 80 μmol/m²s, at the temperature of 15-22 ℃;

in the above preferred embodiment, the light intensity of the bottom-illuminated culture unit 2 of the step (b) is 72. mu. mol/m²s, temperature 20 ℃. In a preferred embodiment, the bottom illumination culture unit 2 is adapted to further germinate by allowing spores or gametes to sit on a bottom dish by utilizing phototaxis.

The technical solution of the present invention will be further described with reference to the following examples.

Example 1

As shown in figure 1, the enteromorpha multi-generation continuous culture device comprises a light culture unit 1, a bottom light culture unit 2 and a suction filtration unit 3;

the suction filtration unit 3 comprises a water pump with a filter screen 31 and is used for suction filtration of enteromorpha spores or gametes in the illumination culture unit 1 into the base illumination culture unit 2;

the base illumination culture unit 2 comprises an attachment matrix 21 for attachment of enteromorpha spores or gametes.

The enteromorpha multi-generation continuous culture device provided by the embodiment mainly comprises an illumination culture unit 1, a bottom illumination culture unit 2 and a suction filtration unit 3; the suction filtration unit 3 comprises a water pump with a filter screen 31, and can suction filter enteromorpha spores or gametes in the illumination culture unit 1 into the base illumination culture unit 2; the enteromorpha spores or gametes are attached to the attachment matrix 21 of the base illumination culture unit 2 to grow, so that multi-generation enteromorpha is obtained, and further multi-generation continuous culture of enteromorpha is effectively realized through the culture device.

Referring to fig. 2, in the present embodiment, the under illumination culturing unit 2 further includes an under illumination culturing chamber 22 and an under illumination light source 23. The top of the bottom illumination incubator 22 is covered with black shading cloth, a disposable plate is placed at the bottom as an attached substrate 21, and light transmission and upward light induction of the bottom of the cylinder are performed only by using a bottom illumination light source 23.

With continued reference to FIG. 2, in a preferred embodiment of the present invention, the light culturing unit 1 includes a light culturing chamber 11 and an intense light source 12. The illumination incubator 11 utilizes the strong light source 12 to carry out strong light irradiation stimulation on the enteromorpha to induce the generation of enteromorpha germ cells.

In a preferred embodiment, the screen 31 of the water pump with the screen 31 is a screen 31 with 900-1600 meshes, and the water pump with the screen 31 can ensure that the microscopic propagules filtered to the bottom illumination incubator 22 are basically spores and male and female gametes released by enteromorpha plants.

Referring to fig. 2, in the present embodiment, the culture apparatus further includes a liquid feeding unit 4 and a liquid discharging unit 5; the liquid feeding unit 4 comprises an artificial seawater bottle, and the liquid feeding unit 4 is communicated with the illumination culture unit 1 through a pipeline; the liquid discharge unit 5 comprises a waste liquid cylinder, and the liquid discharge unit 5 is communicated with the base illumination culture unit 2 through a pipeline.

The liquid feeding unit 4 and the liquid discharging unit 5 are used for conveying and discharging liquid of the illumination culture unit 1 and the bottom illumination culture unit 2, and the stability of the enteromorpha culture environment in the culture process is ensured by a water changing method.

Preferably, the culture solution in the artificial seawater bottle has the same composition as the culture solution in the light irradiation culture unit 1 and the bottom irradiation culture unit 2.

In the above preferred embodiment, peristaltic pumps are arranged on the pipelines of the liquid feeding unit 4 and the light irradiation culture unit 1; peristaltic pumps are arranged on the liquid discharge unit 5 and the pipeline of the bottom illumination culture unit 2.

Preferably, the peristaltic pumps on the pipelines of the liquid feeding unit 4 and the illumination culture unit 1 and the peristaltic pumps on the pipelines of the liquid discharging unit 5 and the bottom illumination culture unit 2 are the same peristaltic pumps.

With continued reference to FIG. 2, the culture apparatus further comprises a carbon dioxide generator 6, and the carbon dioxide generator 6 is in pipe communication with the liquid feeding unit 4 and the light culture unit 1, respectively.

Note: in this embodiment, the light incubator 11 and the bottom light incubator 22 are 600 × 350 × 400mm ultra-white glass jars; the illumination and carbon dioxide system is provided for a Biotron NC Type high-end multifunctional artificial climate box; the water pumps on the pipelines are an integral Peristaltic Pump system Ismatec standard Speed planetary gear drive Digital Pump Ismatec ISM946C IP-N Low-Speed Digital proprietary Pump; 8-Channel.

The specific working process of this embodiment is as follows:

according to the natural occurrence process of enteromorpha green tide in 2008-2020, the enteromorpha is selected as a parent plant to be cultured in a laboratory, and generally the enteromorpha grows on the culture raft in 3 months (stationary growth period), so that the whole enteromorpha with good state and strong cell differentiation capability can be obtained easily.

And (II) putting the whole enteromorpha taken back in the field into a light incubator 11, and culturing by using a common L1 culture solution. The generation of germ cells is stimulated by intense light irradiation. The culture conditions are referred to in table 1 below.

And (3) pumping and filtering water in the illumination culture box 11 to the bottom illumination culture box 22 by using a peristaltic pump, wherein 1600-mesh bolting silk is selected during pumping and filtering, so that the microscopic propagules filtered to the bottom illumination culture box 22 are basically spores and male and female gametes released by enteromorpha plants. The culturing conditions of the under-illumination incubator 22 are similar to those of the illumination incubator 11, and only the illumination conditions are different.

The top of the bottom illumination incubator 22 is covered by black shading cloth, the bottom is provided with a disposable plate, only the bottom of the incubator is upward light-transmitting for light induction, and the light intensity is set to 72 mu mol/m²And(s) according to the phototaxis of the spores and the male and female gametes, the spores and the male and female gametes move to the bottom plate to be fixed, and 30-60 days are required from fixing to growing of macroscopic plants. The plantlets can now be peeled from the plate and moved to the light incubator 11 for cultivation, and so forth in cycles. This ensures that the new enteromorpha is generated by sexual reproduction (combination of male and female gametes) or parthenogenesis (development of only male gametes or female gametes), and ensures the generation continuity.

Referring to FIGS. 3-5, FIG. 3 is a diagram of the spore and male and female gametes of this example swimming onto the bottom plate and fixing;

FIG. 4 is a diagram of an Enteromorpha prolifera in this example, which is peeled from the plate and then moved to the illumination incubator 11 for cultivation; as can be seen from FIG. 4, no significant air sac structure was produced by Enteromorpha at this stage.

FIG. 5 is a diagram of a real Enteromorpha prolifera object obtained by culturing Enteromorpha prolifera in the illumination incubator 11 for 7-14 days; as can be seen from FIG. 5, at this time, the branches of Enteromorpha prolifera are longer, and the main branches are still visible, so that different plants can be still distinguished in the subsequent experimental process, and the generation continuity is further ensured.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.