阅读说明：本技术 一种淋巴灌注显示方法 (Lymph perfusion display method ) 是由 周鸿鹰 汪贵明 刘灵军 熊航 易韬 陈国庆 于 2021-08-10 设计创作，主要内容包括：本发明公开了一种淋巴灌注显示方法,涉及淋巴灌注显示技术领域,其技术方案要点是：S1、选取恒河猴新鲜标本；S2、配制亚甲蓝显色剂；S3、制备明胶凝胶；S4、定位淋巴结；S5、淋巴结灌注,边推注边观察,避免推注压力过大穿破淋巴结,至肉眼可见淋巴结显示蓝色并向心行进；S6、蘸取明胶凝胶将进针点封堵；S7、反复多次步骤S5至S6的灌注显示,直到淋巴管显示完全。本发明采用亚甲蓝水溶液进行淋巴灌注,可显示微细的淋巴管,能避免传统墨汁等在逆压力灌注过程中会很快冲破淋巴管壁,造成筋膜间弥散的问题；通过在恒河猴标本的体内注射显示淋巴导管,能明确人体淋巴回流的具体模式,同时结合临近标志结构进行记录描述,可为手术新术式提供理论指导。(The invention discloses a lymph perfusion display method, which relates to the technical field of lymph perfusion display, and has the technical scheme key points that: s1, selecting fresh rhesus monkey specimens; s2, preparing a methylene blue color developing agent; s3, preparing gelatin gel; s4, positioning lymph nodes; s5, lymph node perfusion is carried out, and the lymph node is observed while injecting, so that the lymph node is prevented from being broken by excessive injection pressure until the lymph node is blue and moves centripetally; s6, dipping gelatin gel to block the needle insertion point; s7, repeating the perfusion display of steps S5 to S6 a plurality of times until the lymphatic vessel display is complete. The invention adopts methylene blue water solution to carry out lymph perfusion, can display fine lymphatic vessels, and can avoid the problem that the traditional Chinese ink and the like can quickly break through the lymphatic vessel wall in the process of back pressure perfusion to cause inter-fascia diffusion; the lymphatic vessel is displayed by injecting in the body of the rhesus monkey specimen, so that the specific mode of the lymphatic reflux of the human body can be determined, and meanwhile, the record description is carried out by combining with the adjacent mark structure, so that the theoretical guidance can be provided for the new surgical operation.)

1. A lymph perfusion display method is characterized in that: the method specifically comprises the following steps:

s1, selecting fresh rhesus monkey specimens;

s2, preparing a methylene blue color developing agent, and uniformly mixing methylene blue powder and pure water to prepare a methylene blue aqueous solution, wherein the ratio of methylene blue is 0.25%;

s3, preparing gelatin gel, mixing gelatin particles with pure water, and continuously heating to obtain gelatin gel;

s4, longitudinally cutting the skin of the inguinal region of the fresh rhesus monkey specimen by using a No. 23 scalpel, separating the soft tissue of the fresh rhesus monkey specimen by using dissecting forceps in an operation mode, and positioning lymph nodes;

s5, lymph node perfusion, wherein a 1ml syringe is connected with a 32G needle, a methylene blue aqueous solution is sucked into the syringe, the syringe and the skin of the inguinal region of a fresh rhesus monkey specimen are in an angle of 45-60 degrees, the syringe is inserted into the positioned lymph node, the methylene blue solution is slowly injected in a centripetal direction, the lymph node is prevented from being punctured by injecting the solution while observing the solution, and the lymph node is prevented from being punctured due to overlarge injection pressure until the lymph node is visible to naked eyes and is blue and advances in the centripetal direction;

s6, plugging the needle points, loosening hands when the lymph nodes are quickly broken by injecting methylene blue water solution until the lymph nodes are quickly burst by pressure, and quickly plugging the needle points by dipping gelatin gel with a cotton swab;

s7, using dissecting forceps to trace the trend of the lymphatic vessels, continuing the injection after the upper stage lymph nodes are determined, and repeating the perfusion display of the steps S5 to S6 for a plurality of times until the lymphatic vessels are completely displayed.

2. The lymphatic perfusion display method of claim 1, wherein: the fresh rhesus monkey specimen described in step S1 was prepared from a rhesus monkey dead in inflammatory disease.

3. The lymphatic perfusion display method of claim 1, wherein: in step S5, when the bolus injection process is blocked, the injector is withdrawn or the bolus injection is stopped, and the pressure of the injector is kept unchanged, and then the bolus injection process is slowed.

Technical Field

The invention relates to the technical field of lymph perfusion display, in particular to a lymph perfusion display method.

Background

With the rapid development of observation means and related subject technologies, the current methods for observing lymphatic vessels mainly comprise a lymphatic vessel injection method, a lymphatic vessel angiography method, an intra-arterial ink-silver nitrate aqueous solution injection method, a lymphatic vessel isotope scanning method, a hydrogen peroxide smearing method, an optical microscope observation method, a scanning electron microscope observation method and the like. The methods have respective characteristics and respectively obtain good effects in practical application. The lymph duct perfusion is a technology that a colored injection is directly injected into the lymph node of a human, or the lymph duct in the field can absorb larger particles, and a pigment is injected into the tissue and absorbed into the lymph duct to enable the lymph duct and the lymph node to be full of color.

In the traditional technology, the lymphatic vessel perfusion display method generally adopts ink, oil painting pigment and the like for perfusion, the ink and oil painting pigment have large particles and large molecular weight, can not pass through certain tissues, and has poor display effect, and meanwhile, the ink, the oil painting pigment and the like can quickly break through the lymphatic vessel wall in the process of reverse pressure perfusion to cause the problem of inter-fascia dispersion.

Therefore, the present invention is directed to provide a lymphatic perfusion display method to solve the above problems.

Disclosure of Invention

The invention aims to provide a lymphatic perfusion display method based on solving the problems that the prepared ink and oil painting pigment particles are large, the molecular weight is large, the prepared ink and oil painting pigment particles cannot pass through certain tissues and the display effect is poor, and meanwhile, the prepared ink and oil painting pigment particles can quickly break through the lymphatic wall in the process of reverse pressure perfusion to cause dispersion among fascia, and the invention adopts methylene blue aqueous solution to carry out lymphatic perfusion to display fine lymphatic vessels and can avoid the problem that the traditional prepared ink particles can quickly break through the lymphatic wall in the process of reverse pressure perfusion to cause dispersion among fascia; in the process of lymph node perfusion, the lymph node state is slowly injected and continuously observed in the whole process, so that the condition that the penetrating lymph node methylene blue water solution is leaked from surrounding soft tissues to influence the perfusion display effect can be prevented; the lymphatic vessel is displayed by injecting in the body of the rhesus monkey specimen, so that the specific mode of the lymphatic reflux of the human body can be determined, and meanwhile, the record description is carried out by combining with the adjacent mark structure, so that the theoretical guidance can be provided for the new surgical operation.

The technical purpose of the invention is realized by the following technical scheme: a lymph perfusion display method specifically comprises the following steps:

s1, selecting fresh rhesus monkey specimens;

s2, preparing a methylene blue color developing agent, and uniformly mixing methylene blue powder and pure water to prepare a methylene blue aqueous solution, wherein the ratio of methylene blue is 0.25%;

s3, preparing gelatin gel, mixing gelatin particles with pure water, and continuously heating to obtain gelatin gel;

s4, longitudinally cutting the skin of the inguinal region of the fresh rhesus monkey specimen by using a No. 23 scalpel, separating the soft tissue of the fresh rhesus monkey specimen by using dissecting forceps in an operation mode, and positioning lymph nodes;

s5, lymph node perfusion, wherein a 1ml syringe is connected with a 32G needle, a methylene blue aqueous solution is sucked into the syringe, the syringe and the skin of the inguinal region of a fresh rhesus monkey specimen are in an angle of 45-60 degrees, the syringe is inserted into the positioned lymph node, the methylene blue solution is slowly injected in a centripetal direction, the lymph node is prevented from being punctured by injecting the solution while observing the solution, and the lymph node is prevented from being punctured due to overlarge injection pressure until the lymph node is visible to naked eyes and is blue and advances in the centripetal direction;

s6, plugging the needle points, loosening hands when the lymph nodes are quickly broken by injecting methylene blue water solution until the lymph nodes are quickly burst by pressure, and quickly plugging the needle points by dipping gelatin gel with a cotton swab;

s7, using dissecting forceps to trace the trend of the lymphatic vessels, continuing the injection after the upper stage lymph nodes are determined, and repeating the perfusion display of the steps S5 to S6 for a plurality of times until the lymphatic vessels are completely displayed.

Further, the fresh rhesus monkey specimen described in step S1 was prepared from a rhesus monkey dead in inflammatory disease.

Further, in step S5, when a blockage is encountered during the bolus injection, the syringe is withdrawn or the bolus injection is stopped, and the pressure of the syringe is kept unchanged, and then the bolus injection is slowly advanced.

In conclusion, the invention has the following beneficial effects:

1. according to the method, rhesus monkeys with inflammatory disease death are adopted to prepare fresh rhesus monkey specimens for lymph perfusion display, and lymph nodes of the specimens are swollen, large in size and convenient to inject and perfuse;

2. according to the method, the methylene blue aqueous solution is adopted for lymph perfusion, the methylene blue aqueous solution has good perfusion fluency and low resistance, can display fine lymphatic vessels, and can avoid the problem that the traditional Chinese ink and the like can quickly break through the lymphatic vessel wall in the process of reverse pressure perfusion to cause inter-fascia dispersion;

3. in the lymph node perfusion process, the method slowly injects the liquid in the whole process and continuously observes the lymph node state, so that the lymph node is prevented from being punctured, and the methylene blue aqueous solution is prevented from seeping from surrounding soft tissues to influence the perfusion display effect;

4. the method of the invention can show the lymphatic vessel by injecting in the body of the rhesus monkey specimen, can determine the specific mode of the lymphatic return of the human body, and can provide theoretical guidance for the new surgical operation by recording and describing the structure of the adjacent mark.

Drawings

FIG. 1 is a flow chart in an embodiment of the invention;

FIG. 2 is a schematic diagram of the operational state in an embodiment of the present invention;

FIG. 3 is a diagram illustrating the effect of perfusion display in an embodiment of the present invention.

Detailed Description

The present invention is described in further detail below with reference to figures 1-3.

Example (b): a lymphatic perfusion display method, as shown in fig. 1, specifically comprising the following steps:

s1, selecting fresh rhesus monkey specimens, wherein the selected fresh rhesus monkey specimens are the best specimens for inflammatory diseases and the specimens for inflammatory diseases, and have swollen lymph nodes, large volume and convenient injection and perfusion;

s2, preparing a methylene blue color developing agent, and uniformly mixing methylene blue powder and pure water to prepare a methylene blue aqueous solution, wherein the ratio of methylene blue is 0.25%;

s3, preparing gelatin gel, mixing gelatin particles with pure water, and then continuously heating to prepare the gelatin gel, wherein the prepared gelatin gel is convenient for blocking a needle hole in the subsequent perfusion operation, so that liquid is prevented from spreading out to influence the deformation of lymphatic vessels and tissues observed by the vessels;

s4, longitudinally cutting the skin of the inguinal region of the fresh rhesus monkey specimen by using a No. 23 scalpel, separating the soft tissue of the fresh rhesus monkey specimen by using dissecting forceps in an operation mode, positioning lymph nodes, and exposing the lymph nodes;

s5, lymph node perfusion, wherein a 1ml syringe is connected with a 32G needle, a methylene blue aqueous solution is sucked into the syringe, the syringe and the skin of the inguinal region of a fresh rhesus monkey specimen are in an angle of 45-60 degrees and are inserted into the positioned lymph node, the methylene blue solution is slowly injected in the centripetal direction, the lymph node is prevented from being punctured by injecting the solution while observing the solution, and the lymph node is prevented from being punctured due to excessive injection pressure until the lymph node is visible to naked eyes and is blue and advances centripetally;

s6, plugging the needle points, loosening hands when the lymph nodes are quickly broken by injecting methylene blue water solution until the lymph nodes are quickly burst by pressure, and quickly plugging the needle points by dipping gelatin gel with a cotton swab;

s7, using dissecting forceps to trace the trend of the lymphatic vessels, continuing the injection after the upper stage lymph nodes are determined, and repeating the perfusion display of the steps S5 to S6 for a plurality of times until the lymphatic vessels are completely displayed.

In step S5, when the bolus injection process is blocked, the injector is withdrawn or the bolus injection is stopped, and the pressure of the injector is kept unchanged, and then the bolus injection process is slowed.

In this embodiment, Methylene blue (also called Methylene blue or Methylene blue) is an aromatic heterocyclic compound. Methylene blue is a blue basic dye that binds nucleic acids, while cellular DNA is readily stained by basic dyes, rendering the nucleus blue. Furthermore, methylene blue can be used for anti-inflammation, anti-oxidation, tumor photothermal therapy and the like in clinic, and the aqueous solution of the methylene blue can be directly used for imaging of living lymphatic vessels.

At present, the technical bottleneck of clinical lymph contrast is that no proper contrast agent is available, and a feasible contrast agent injection point is not determined. The specific mode of the lymphatic reflux of the human body can be determined by injecting and displaying the lymphatic vessels in the rhesus monkey body, and meanwhile, the recording description is carried out by combining the adjacent mark structure, so that theoretical guidance can be provided for a new surgical operation.

In the embodiment of the invention, the methylene blue aqueous solution is adopted for lymph perfusion, the methylene blue aqueous solution has good perfusion fluency and low resistance, can display fine lymphatic vessels, and can avoid the problem that the traditional Chinese ink and the like can quickly break through the lymphatic vessel wall in the process of reverse pressure perfusion to cause inter-fascia dispersion; in addition, in the lymph node perfusion process, the lymph node state is slowly injected and continuously observed in the whole process, so that the lymph node is prevented from being punctured, and the methylene blue aqueous solution is prevented from seeping from surrounding soft tissues to influence the perfusion display effect; in addition, the specific mode of the lymphatic return of the human body can be determined by selecting the lymphatic vessel displayed by in-vivo injection of the rhesus monkey specimen, and meanwhile, the record description is carried out by combining the adjacent mark structure, so that theoretical guidance can be provided for a new surgical mode.

The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.