阅读说明：本技术 一种电磁耦合发酵淡紫链霉菌海南变种微生物生物电池 (Electromagnetic coupling fermentation streptomyces lavendulae Hainan variant microorganism biological battery ) 是由 潘忠成 沈伟杰 赵珺涵 翁婧 陈豪 师维 李蒲民 于 2021-09-14 设计创作，主要内容包括：本发明涉及一种电磁耦合发酵淡紫链霉菌海南变种微生物生物电池,所述微生物生物电池包括阳极碳棒,阴极碳棒,摇瓶培养基,定值电阻,可变电阻,质子交换膜,废水配置液,外加电场,工作站,pH传感器,溶解氧传感器,搅拌器,充氧装置,外加磁场,阳极碳棒接种淡紫链霉菌海南变种。本发明采用电磁耦合环境下可以提高淡紫链霉菌的生产效价的同时还能降解生物农药在发酵生产过程的废水。(The invention relates to an electromagnetic coupling fermentation Streptomyces lavendulae Hainan variant microbial cell which comprises an anode carbon rod, a cathode carbon rod, a shake flask culture medium, a fixed value resistor, a variable resistor, a proton exchange membrane, a wastewater preparation liquid, an external electric field, a workstation, a pH sensor, a dissolved oxygen sensor, a stirrer, an oxygenating device and an external magnetic field, wherein the anode carbon rod is inoculated with the Streptomyces lavendulae Hainan variant. The invention can improve the production titer of the streptomyces lavendulae and degrade the wastewater of the biological pesticide in the fermentation production process under the electromagnetic coupling environment.)

1. An electromagnetic coupling fermentation Hainan variant microbial cell of streptomyces lavendulae is characterized by comprising an anode carbon rod, a cathode carbon rod, a shake flask culture medium, a fixed-value resistor, a variable resistor, a proton exchange membrane, a wastewater preparation liquid, an external electric field, a workstation, a pH sensor, a dissolved oxygen sensor, a stirrer, an oxygenating device and an external magnetic field, wherein the anode carbon rod, the pH sensor, the dissolved oxygen sensor and the stirrer are positioned in the shake flask culture medium, the cathode carbon rod is positioned in the wastewater preparation liquid, the anode carbon rod and the cathode carbon rod are electrically connected with the variable resistor through the fixed-value resistor, the proton exchange membrane is positioned between the shake flask culture medium and the wastewater preparation liquid, the shake flask culture medium and the wastewater preparation liquid are positioned between the external electric field, the shake flask culture medium and the wastewater preparation liquid are positioned between the external magnetic field, and the oxygenating device is connected with the shake flask culture medium, inoculating Hainan variety of Streptomyces lavendulae to the carbon rod of the anode.

2. The microbial bio-cell of claim 1, further comprising an acid and/or base addition flask for conditioning the shake flask culture medium, and a vessel for automatic feeding of the shake flask culture medium.

3. The microbial bio-cell of claim 1, wherein both said applied magnetic field and said applied electric field are adjustable.

4. The microbial bio-battery according to claim 3, wherein the magnetic induction of said externally applied magnetic field is adjustable within a range of 0.1-0.6T.

5. The device of claim 3, wherein the applied electric field is adjusted by a DC power supply, and the current intensity of the applied electric field is adjustable within a range of 0-100 mA.

6. A method for simultaneously producing zhongshengmycin and treating wastewater, which is characterized by adopting the microbial biological battery of any one of claims 1 to 5, wherein the zhongshengmycin is obtained from a shake flask culture medium, and the wastewater is treated in a wastewater preparation solution.

7. The method as claimed in claim 6, wherein the wastewater preparation solution is the wastewater from the production of kasugamycin by biological fermentation, and the initial COD concentration of the wastewater is 600-700mg/mL and the initial ammonia nitrogen concentration is 200-300 mg/mL.

8. The method of claim 6, wherein the shake flask culture medium is formulated as: soluble starch (AR) 1.0%, MgSO₄·7H₂O（AR）0.045%、KH₂PO₄（AR）0.10%、KCl（AR）0.045%、NaNO₃（AR）0.125%、FeSO₄·7H₂O (AR) 0.001%, and distilled water in balance, and has a pH of 6.8-7.0.

9. The method as claimed in claim 6, wherein the shaking culture medium is stirred at a speed of 120-160rmp, the culture temperature is 28-30 ℃, and the aeration rate per minute is 1:1-2(V/V) of sterilized air; the automatic refueling amount is 1/5-1/3 of the liquid volume of the anode chamber per day.

10. The method according to any one of claims 6 to 9, wherein the direct current is 30 to 50mA and the magnetic field is 0.15 to 0.25T.

Technical Field

The invention belongs to the field of microbial pharmacy, and particularly relates to a Hainan variant microbial battery prepared by fermenting Streptomyces lavendulae through electromagnetic coupling and application of the Hainan variant microbial battery.

Background

Zhongshengmycin (Zhongshengmycin) belongs to N-glucoside agricultural antibiotics, contains 6 effective components, wherein a beta-lysine is added between each component in turn, the activity is realized on bacteria, saccharomycetes and filamentous fungal diseases, a biological bactericide formed by processing and preparing the Zhongshengmycin is an environment resource protection type biological sterilization treatment medicine, the medicine has good inhibition or effective inactivation effect on various gram rod-shaped positive bacteria, gram rod-shaped negative bacteria and filamentous negative fungi, the application of the Zhongshengmycin in agriculture is very wide, and the Zhongshengmycin is mainly used for preventing and treating rice bacterial streak, streak disease, bacterial leaf blight, black rot, soft leather disease and red pepper disease of Chinese cabbage, purple canker disease of cucumber, ginger blast and angular leaf spot disease, perforation disease of peach tree, apricot tree and plum tree, bacterial influenza of kidney bean and the like; the bactericide has low toxicity to human and livestock and no pollution to environment, and is an ideal biological pesticide with antibacterial and antifungal effects.

Compared with other novel prior art which utilizes organic production capacity, the Microbial Fuel Cell (MFC) has the characteristics and functional advantages of simplicity, convenience and easiness in operation: firstly, they directly transform the original matrix into electricity, ensuring high energy conversion; second, unlike all current bio-energy treatment methods, microbial fuel solar cells can operate effectively in the environment; third, the microbial cells do not have to be treated with exhaust gas, because the main emission component of the exhaust gas generated in the cells is carbon dioxide, and generally, none of these has a reusable energy source; fourthly, the microbial fuel cell does not need to input much energy into the air, because a single-chamber microbial cell only needs ventilation by air alone, and can passively supplement anode gas into the air; fifth, in some countries where the countries are still poor, because the countries are still poor at present, the governments of China do not have enough funds to support the development of microbial fuel solar cells, and the fuels in the development are more diversified. Despite the advantages of the microbial biological battery, the microbial biological battery has insufficient utilization of anode microorganisms and exploitation of biological value, the anode microorganisms only transport redundant electrons generated by metabolism to a cathode chamber through an anode region of an anode chamber and an external connecting lead in the conventional biological battery, the oxygen and the wastewater substrate in the cathode chamber are used for decomposing wastewater organic matters, although the device has two functions of degrading cathode wastewater and generating electricity, however, metabolites produced by microbial fermentation in the anode chamber are often ignored, even the microbial fermentation products in the anode chamber are treated as three wastes, so that the utilization of the anode microbial value of the microbial cell is greatly influenced, and huge resource waste is caused.

Disclosure of Invention

In order to solve the problems of the prior art that the Streptomyces lavendulae Hainan variety (Streptomyces lavendulae var. hainanensis new var.) The invention provides a microbial cell for electromagnetically coupling and fermenting Hainan variant streptomyces lavendulae, which solves the technical problem of low content of zhongshengmycin in the existing strain. The starting strain of the invention is a Hainan variety of Streptomyces lavendulae (a)Streptomyces lavendulae var. hainanensis new var.)。

More specifically, the invention relates to an electromagnetic coupling fermentation streptomyces lavendulae variety microbial cell, which is characterized by comprising an anode carbon rod, a cathode carbon rod, a shake flask culture medium, a fixed value resistor, a variable resistor, a proton exchange membrane, a wastewater preparation liquid, an external electric field, a workstation, a pH sensor, a dissolved oxygen sensor, a stirrer, an oxygenating device and an external magnetic field, wherein the anode carbon rod, the pH sensor, the dissolved oxygen sensor and the stirrer are positioned in the shake flask culture medium, the cathode carbon rod is positioned in the wastewater preparation liquid, the anode carbon rod and the cathode carbon rod are electrically connected with the variable resistor through the fixed value resistor, the proton exchange membrane is positioned between the shake flask culture medium and the wastewater preparation liquid, the shake flask culture medium and the wastewater preparation liquid are positioned between the external electric field, the shake flask culture medium and the wastewater preparation liquid are positioned between the external magnetic field, and the oxygenating device is connected with the shake flask culture medium, inoculating Hainan variety of Streptomyces lavendulae to the carbon rod of the anode.

In a preferred embodiment of the present invention, the microbial bio-cell further comprises an acid and/or base addition flask for adjusting the shake flask culture medium, and a vessel for automatic feeding of the shake flask culture medium.

In a preferred embodiment of the invention, the external magnetic field and the external electric field are adjustable.

In a preferred embodiment of the present invention, the magnetic induction of the external magnetic field is adjustable within a range of 0.1 to 0.6T, preferably 0.15 to 0.25T.

In a preferred embodiment of the invention, the external electric field is adjusted by a direct current power supply, and the adjustable range of the current intensity is 0-100 mA.

The invention also relates to a method for simultaneously producing the zhongshengmycin and treating wastewater, which is characterized by adopting the microbial biological battery, wherein the zhongshengmycin is obtained from a shake flask culture medium, and the wastewater is treated in a wastewater preparation solution.

In a preferred embodiment of the invention, the wastewater preparation liquid is wastewater for producing kasugamycin by biological fermentation, the initial COD concentration of the wastewater is 600-. Preferably, the COD reduction rate of the treated wastewater preparation liquid is over 95 percent, preferably over 98 percent, and the ammonia nitrogen reduction rate is over 90 percent, preferably over 94 percent.

In a preferred embodiment of the present invention, the formulation of the shake flask culture medium is: solubility in waterStarch (AR) 1.0%, MgSO₄·7H₂O（AR）0.045%、KH₂PO₄（AR）0.10%、KCl（AR）0.045%、NaNO₃（AR）0.125%、FeSO₄·7H₂O (AR) 0.001%, and distilled water in balance, and has a pH of 6.8-7.0.

In a preferred embodiment of the invention, the shaking flask culture medium is stirred at the speed of 120-160rmp, the culture temperature is 28-30 ℃, and the aeration rate per minute is 1:1-2(V/V) of sterile air; the automatic refueling amount is 1/5-1/3 of the liquid volume of the anode chamber per day.

In a preferred embodiment of the present invention, the direct current is 30 to 50mA, and the magnetic field is 0.15 to 0.25T.

Further, the culture broth of the S.lavendulae Hainan variant mutant strain inoculated is 5-15% (V/V), preferably 10% of the culture medium in claim 6; the stirring speed of the anode chamber is 120-160rmp, preferably 150rmp, the culture temperature is 28-30 ℃, and the ventilation per minute is 1:2-1:4(V/V) of sterile air, preferably 1: 1; the automatic refueling amount is 1/10-1/3, preferably 1/5 of the liquid volume of the anode chamber per day; the culture time is 72-128h, preferably 84 h.

Compared with the prior art, the invention has the following advantages: on one hand, the production titer of the streptomyces lavendulae can be improved under the electromagnetic coupling environment, on the other hand, the MFC constructed by the device can be used for degrading the wastewater of the biological pesticide in the fermentation production process, the construction effect of the device is obviously different from that of the conventional biological battery, the device can fully excavate the anode microorganism economic benefit of the MFC, and the device can also treat the wastewater of the cathode region of the MFC at the same time.

Drawings

The following is further described with reference to the accompanying drawings:

FIG. 1 is a schematic structural diagram of a Hainan variant microbial bio-cell for electromagnetically-coupled fermentation of Streptomyces lavendulae, wherein 1-an anode carbon rod, 2-a cathode carbon rod, 3-a shake flask culture medium, 4-a customized resistor, 5-a variable resistor, 6-a proton exchange membrane, 7-a wastewater artificial preparation liquid, 8-an external electric field, 9-a workstation, 10-a pH sensor, 11-a dissolved oxygen sensor, 12-a stirrer, 13-an HCl (0.1mol/L) micro-adding bottle, 14-an NaOH (0.1mol/L) micro-adding bottle, 15-an automatic feeding bottle, 16-an oxygenating device and 17-an external magnetic field;

FIG. 2 shows the change of zhongshengmycin and mitochondrial enzyme in Hainan variant of Streptomyces lavendulae fermented without electric field and magnetic field;

FIG. 3. Zhongshengmycin and mitochondrial enzyme changes from Hainan variant of Streptomyces lavendulae with the addition of 20 mA;

FIG. 4. Zhongshengmycin and mitochondrial enzyme changes from S.lavendulae variant with an additional 40 mA;

FIG. 5. variation of zhongshengmycin and mitochondrial enzymes from Hainan variety of Streptomyces lavendulae with the addition of 80 mA;

FIG. 6 shows the change of zhongshengmycin and mitochondrial enzymes of Hainan variant of Streptomyces lavendulae fermented under an applied magnetic field of 40mA and 0.1T;

FIG. 7 shows the change of zhongshengmycin and mitochondrial enzymes of Hainan variant of Streptomyces lavendulae fermented under an applied magnetic field of 40mA and 0.2T;

FIG. 8 shows the change of zhongshengmycin and mitochondrial enzymes of Hainan variant of S.lavendulae fermented under an applied magnetic field of 40mA and 0.4T.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not to be construed as limiting the invention.

The invention is further illustrated by the following examples:

example 1: inoculating Streptomyces lavendulae in MFC anode region, wherein the anode inoculation liquid comprises glucose (AR) 1.5%, cold-pressed soybean cake powder (IR) 2.0%, corn Flour (FR) 0.15%, corn starch (FR) 1.5%, and NH₄Cl（AR）0.45%、NaCl（AR）0.35%、MgSO₄·7H₂O（AR）0.03%、KH₂PO₄(AR) 0.03% light CaCO₃(AR) 0.04% and tap water, wherein the pH value before elimination of the shake flask seed culture medium is 6.5-7.5, the fermentation temperature of the zhongshengmycin is 25 ℃, the ventilation capacity is 600-650L/min, the stirring speed is 120rmp, the inoculation amount of the streptomyces lavendulae is 10%, and the supplement is providedThe material amount is 3 mL/L.h, the cathode liquid is wastewater of Shaanxi Mackelukasugamycin fermentation workshop, the COD initial concentration of the wastewater is 650mg/L, and the ammonia nitrogen concentration is 65mg/L, so that the MFC of the streptomyces lavendulae is constructed.

Example 2:

inoculating Streptomyces lavendulae in MFC anode region, wherein the anode inoculation liquid comprises glucose (AR) 1.5%, cold-pressed soybean cake powder (IR) 2.0%, corn Flour (FR) 0.15%, corn starch (FR) 1.5%, and NH₄Cl（AR）0.45%、NaCl（AR）0.35%、MgSO₄·7H₂O（AR）0.03%、KH₂PO₄(AR) 0.03% light CaCO₃(AR) 0.04% and tap water, wherein the pH value of the shake flask seed culture medium before digestion is 6.5-7.5, the fermentation temperature of the zhongshengmycin is 25 ℃, the ventilation quantity is 600-650L/min, the stirring speed is 120rmp, the inoculation quantity of the streptomyces lavendulae is 10%, the supplement quantity is 3 mL/L.h, the change trend of the zhongshengmycin F content along with the extension of the fermentation time in the absence of an external electric field is increased firstly and then basically unchanged and then reduced, and the maximum value is reached after the fermentation time is 24 h. The change trend of the content of the mitochondrial enzyme I along with the extension of the fermentation time is gradually increased and then reduced, and the content reaches the maximum value about 72 hours of the fermentation time. The trend of the mitochondrial enzyme II content with increasing fermentation time was continuously and slowly increasing. The change trend of the content of the mitochondrial enzyme III along with the extension of the fermentation time is gradually increased and then reduced, and reaches a maximum value around 36h of the fermentation time (the specific change is shown in figure 2).

The method for measuring the mitochondrial enzyme in the fermentation process of the Hainan variant mutant strain of the Streptomyces lavendulae is characterized by mainly comprising the following steps of: a: pretreating fermentation liquor of Hainan variant mutant strains of Streptomyces lividans: taking 0.5-2mL of fermentation liquid in different fermentation periods, preferably 0.9 mL; the centrifugal speed of 15000 and 20000rmp, preferably 15000 rmp; ultracentrifuging for 10-20min, preferably 15 min; taking the supernatant as a mitochondrial complex enzyme system sample for testing the Hainan variant mutant strain of the streptomyces lavendulae; b: the method for determining the mitochondrial complex enzyme I of the Hainan variant mutant strain of the Streptomyces lividans comprises the following steps: taking 900 microliter of the sample treated by A, adding 100 microliter of glycylglycine buffer solution (6mmol/L NADH 0.2mmol/L, glycylglycine 0.5mmol, cytochrome C, pH8.5, reacting for 2 minutes, measuring the absorbance at 550nm of an ultraviolet spectrophotometer, and measuring C: 900 microliter of the mitochondrial complex enzyme II of the Hainan variant mutagenized strain of the Streptomyces avermitilis by taking 100 microliter of the sample treated by A, adding buffer solution (0.03M/L potassium iron hydride 0.2mmol/LPBS, 1% bovine serum albumin and 0.6mol/L succinic acid) 900 microliter of the sample treated by A, reacting for 2 minutes, measuring at 420nm of the ultraviolet spectrophotometer, and measuring D: the mitochondrial complex enzyme III of the Hainan variant mutagenized strain of the Streptomyces avermitilis is measured by adding 100 microliter of the sample treated by A, adding 900 microliter of buffer solution (0.3mmol/L cytochrome C) to 75mM/L of phosphate buffer solution, reacting for 2 minutes, measuring the light absorption value at 550nm of an ultraviolet spectrophotometer

Example 3:

inoculating Streptomyces lavendulae in MFC anode region, wherein the anode inoculation liquid comprises glucose (AR) 1.5%, cold-pressed soybean cake powder (IR) 2.0%, corn Flour (FR) 0.15%, corn starch (FR) 1.5%, and NH₄Cl（AR）0.45%、NaCl（AR）0.35%、MgSO₄·7H₂O（AR）0.03%、KH₂PO₄(AR) 0.03% light CaCO₃(AR) 0.04% and tap water, wherein the pH value before the extinction of the shake flask seed culture medium is 6.5-7.5, the fermentation temperature of the zhongshengmycin is 25 ℃, the ventilation quantity is 600-650L/min, the stirring speed is 120rmp, the inoculation quantity of the streptomyces lavendulae is 10%, the supplement quantity is 3 mL/L.h, the change situation of the zhongshengmycin content along with the fermentation time under the action of an external electric field of 20mA is shown in figure 3, the change trend of the zhongshengmycin F content along with the extension of the fermentation time is basically unchanged after the zhongshengmycin F content is firstly increased when 20mA direct current is applied to the medium, and the maximum value is reached after the fermentation time is 36 h. The trend of the mitochondrial enzyme I content with increasing fermentation time is continuously increasing. The trend of the mitochondrial enzyme II content with increasing fermentation time was continuously and slowly increasing. The change trend of the content of the mitochondrial enzyme III along with the extension of the fermentation time is gradually increased and then reduced, and the content reaches the maximum value about 72 hours of the fermentation time.

Example 4:

inoculating Streptomyces lavendulae in MFC anode region, wherein the anode inoculation liquid comprises glucose (AR) 1.5%, cold-pressed soybean cake powder (IR) 2.0%, and corn Flour (FR)0.15%, corn starch (FR) 1.5%, NH₄Cl（AR）0.45%、NaCl（AR）0.35%、MgSO₄·7H₂O（AR）0.03%、KH₂PO₄(AR) 0.03% light CaCO₃(AR) 0.04% and tap water, wherein the pH value of the shake flask seed culture medium before elimination is 6.5-7.5, the fermentation temperature of the zhongshengmycin is 25 ℃, the ventilation quantity is 600-650L/min, the stirring speed is 120rmp, the inoculation quantity of the streptomyces lavendulae is 10%, the supplement quantity is 3 mL/L.h, the change situation of the zhongshengmycin content along with the fermentation time under the action of an external electric field 40mA is shown in figure 4, and as can be seen from figure 4, the change trend of the zhongshengmycin F content along with the extension of the fermentation time under the external 40mA direct current is basically unchanged after being increased, and the maximum value is reached after the fermentation time is 36 h. The trend of the mitochondrial enzyme I content with increasing fermentation time is continuously increasing. The trend of the mitochondrial enzyme II content with increasing fermentation time was continuously increasing. The trend of the mitochondrial enzyme III content with increasing fermentation time was first slowly increasing.

Example 5:

inoculating Streptomyces lavendulae in MFC anode region, wherein the anode inoculation liquid comprises glucose (AR) 1.5%, cold-pressed soybean cake powder (IR) 2.0%, corn Flour (FR) 0.15%, corn starch (FR) 1.5%, and NH₄Cl（AR）0.45%、NaCl（AR）0.35%、MgSO₄·7H₂O（AR）0.03%、KH₂PO₄(AR) 0.03% light CaCO₃(AR) 0.04% and tap water, wherein the pH value before the extinction of the shake flask seed culture medium is 6.5-7.5, the fermentation temperature of the zhongshengmycin is 25 ℃, the ventilation quantity is 600-650L/min, the stirring speed is 120rmp, the inoculation quantity of the streptomyces lavendulae is 10%, the supplement quantity is 3 mL/L.h, the change situation of the zhongshengmycin content along with the fermentation time under the action of an external electric field of 80mA is shown in figure 5, and the change trend of the zhongshengmycin F content along with the extension of the fermentation time under the action of an external 80mA direct current is continuously reduced from figure 5. The trend of the mitochondrial enzyme I content with increasing fermentation time is continuously decreasing. The trend of the mitochondrial enzyme II content with increasing fermentation time was a continuous slow decrease. The trend of the mitochondrial enzyme III content with increasing fermentation time was first slowly decreasing.

Example 6:

inoculating Streptomyces lavendulae in MFC anode region, wherein the anode inoculation liquid comprises glucose (AR) 1.5%, cold-pressed soybean cake powder (IR) 2.0%, corn Flour (FR) 0.15%, corn starch (FR) 1.5%, and NH₄Cl（AR）0.45%、NaCl（AR）0.35%、MgSO₄·7H₂O（AR）0.03%、KH₂PO₄(AR) 0.03% light CaCO₃(AR) 0.04% and tap water, wherein the pH value before the extinction of the shake flask seed culture medium is 6.5-7.5, the fermentation temperature of the zhongshengmycin is 25 ℃, the ventilation quantity is 600-650L/min, the stirring speed is 120rmp, the inoculation quantity of the streptomyces lavendulae is 10%, the supplement quantity is 3 mL/L.h, the univariate is taken as the direct current setting quantity, the external direct current of 40mA is respectively used, the single variable is taken as the magnetic field setting increment, the external magnetic fields of 0T, 0.1T, 0.2T and 0.4T are respectively used, the two factors are combined in a cross mode, when the external direct current of 40mA and the external magnetic field are 0.2T, the content of the zhongshengmycin F component is highest, the fermentation time is 84h, and the content of the zhongshengmycin is 3.05g/L (see fig. 6, 7 and 8 in particular).

Example 7:

inoculating Streptomyces lavendulae in MFC anode region, wherein the anode inoculation liquid comprises glucose (AR) 1.5%, cold-pressed soybean cake powder (IR) 2.0%, corn Flour (FR) 0.15%, corn starch (FR) 1.5%, and NH₄Cl（AR）0.45%、NaCl（AR）0.35%、MgSO₄·7H₂O（AR）0.03%、KH₂PO₄(AR) 0.03% light CaCO₃(AR) 0.04% and tap water, wherein the pH value before elimination of the shake flask seed culture medium is 6.5-7.5, the fermentation temperature of the zhongshengmycin is 25 ℃, the ventilation volume is 600-year-old 650L/min, the stirring speed is 120rmp, the inoculation amount of the streptomyces lavendulae is 10%, the feeding amount is 3 mL/L.h, the cathode liquid is waste water from Shaanxi Mackeochun Leoeritin fermentation workshops, the COD initial concentration of the waste water is 650mg/L, and the ammonia nitrogen concentration is 65 mg/L. When the external electric field is 40mA and the magnetic field is 0.2T, the COD effluent concentration of the kasugamycin wastewater in the cathode region is 11.8mg/L (the COD reduction rate exceeds 95%, preferably exceeds 98%), the effluent ammonia nitrogen concentration is 3.83mg/L (the ammonia nitrogen reduction rate exceeds 90%, preferably exceeds 94%), and the effluent COD concentration and the ammonia nitrogen concentration are obviously lower than any combination of other external electric field strength and magnetic field strength.

The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that are not thought of through the inventive work should be included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope defined by the claims.