阅读说明：本技术 一种高通量病理切片扫描成像装置 (High-flux pathological section scanning imaging device ) 是由 华道柱 方奕彪 兰敬隆 张立琛 吉海泉 于 2021-08-10 设计创作，主要内容包括：本发明公开了一种高通量病理切片扫描成像装置,包括依次固定设置在机械支架上的光源、光束整形模块、分光器件、透镜组、成像模块,机械支架与运动模块级联在一起,由运动模块带动整个机械支架运动,机械支架对应分光器件的两侧固定有显微物镜及物镜焦距调节模块,显微物镜用于将切片样品的反射光或者荧光反射到透镜组和成像模块进行成像。本发明通过设置的多棱锥或多棱台状结构的分光器件,侧面数量与成像通道对应,通过增大分光器件体积或者增加侧面数量,并匹配更多的成像通道,实现系统成像通道扩增,同一时间更多的通道对样品进行成像,提高切片样品成像装置的通量,提高工作效率。(The invention discloses a high-flux pathological section scanning imaging device which comprises a light source, a light beam shaping module, a light splitting device, a lens group and an imaging module which are sequentially and fixedly arranged on a mechanical support, wherein the mechanical support and a motion module are cascaded together, the motion module drives the whole mechanical support to move, a microscope objective and an objective focal length adjusting module are fixed on two sides of the mechanical support corresponding to the light splitting device, and the microscope objective is used for reflecting reflected light or fluorescence of a section sample to the lens group and the imaging module for imaging. According to the invention, the number of the side surfaces of the arranged light splitting devices with the polygonal pyramid or polygonal frustum structure corresponds to that of the imaging channels, the amplification of the imaging channels of the system is realized by increasing the volume of the light splitting devices or increasing the number of the side surfaces and matching more imaging channels, more channels image the sample at the same time, the flux of the sliced sample imaging device is improved, and the working efficiency is improved.)

1. The utility model provides a high flux pathological section scanning imaging device, its characterized in that is including light source (2), beam shaping module (3), beam splitter (4), battery of lens (5), imaging module (6) of fixed setting on mechanical support (1) in proper order, mechanical support (1) and motion module (7) cascade are in the same place, are driven whole mechanical support (1) motion by motion module (7), mechanical support (1) correspond the both sides of beam splitter (4) and are fixed with microscope objective (8) and objective focus adjusting module (9), microscope objective (8) are used for reflecting the reverberation or the fluorescence of section sample (10) to battery of lens (5) and imaging module (6) and take images.

2. The high-throughput pathological section scanning imaging device as defined in claim 1, wherein the light source (2) is a wide-field light source or a laser light source.

3. A high throughput pathological section scanning imaging device according to claim 1, wherein said beam splitter (4) is a polygonal pyramid-like structure or a polygonal frustum-like structure, each side surface of which has an optical interface (11) for reflecting light from a light source and irradiating it onto the section sample (10) through the microscope objective (8).

4. A high throughput pathological section scanning imaging device according to claim 3, wherein said optical interface (11) is a half-mirror or a dichroic mirror.

5. The high-throughput pathological section scanning imaging device of claim 1, wherein the light source (2), the beam shaping module (3), the beam splitter (4), the lens assembly (5) and the imaging module (6) are located on a straight line.

6. A high throughput pathological section scanning imaging device according to claim 1, characterized in that the section samples (10) are placed in parallel on both sides of the motion module (7) or distributed annularly around the central axis of the mechanical support (1).

7. A high throughput pathological section scanning imaging device according to claim 1, wherein said lens assembly (5) is a combination of multiple lenses or an integrated lens assembly.

8. The high throughput pathological section scanning imaging device according to claim 1, wherein the imaging module (6) is formed by combining a plurality of photodetectors or an integrated photodetector.

Technical Field

The invention belongs to the technical field of medical equipment, and particularly relates to a high-flux pathological section scanning imaging device.

Background

The pathological section imaging device is used for carrying out continuous scanning imaging on pathological sections through an optical microscopic imaging system to obtain a high-resolution cell tissue image, and carrying out functional processing such as splicing, calibration and the like on the obtained microscopic image by applying a computer technology to obtain a complete cell tissue sample digital image. With the development of telemedicine, the demand of pathological section imaging devices is increasing. The pathological section imaging device can realize high-resolution digital imaging of a far-end cell tissue sample, and by matching with the Internet technology and a remote consultation platform, medical experts in different regions can quickly read and analyze slices of different-place patients, so that multiple persons can simultaneously consult the state of an illness of the same patient, the regional boundary of medical resources is broken, and the medical service level of the whole society is improved.

Currently, pathological section scanning devices can be classified into a single-chip type, a multi-chip type, and a high-throughput type according to the number of loaded slices. The multi-sheet type and high-flux type slice scanning device can simultaneously realize unattended imaging of a plurality of pathological slices, and particularly, the high-flux slice scanning device can automatically scan and image hundreds of slice samples. In situations where a large number of pathological sections require digital images, the need for high throughput slice scanning imaging devices is more pressing. Because of the large sample size, the working time of the scanning device is more demanding in order to quickly obtain digitized images of all samples. And the conventional scanning method is used for carrying out continuous scanning imaging on a large number of slice samples one by one, which is long in time consumption. Therefore, it is necessary to develop a high-resolution, high-throughput imaging apparatus and method for pathological section samples by optical imaging system design.

Disclosure of Invention

In view of the problems in the prior art, the present invention is designed to provide a high-throughput pathological section scanning imaging device.

The invention is realized by the following technical scheme:

the high-flux pathological section scanning imaging device is characterized by comprising a light source, a light beam shaping module, a light splitting device, a lens group and an imaging module which are sequentially and fixedly arranged on a mechanical support, wherein the mechanical support and a motion module are cascaded together, the motion module drives the whole mechanical support to move, so that the rapid scanning imaging of a section sample is realized, a microscope objective and an objective focal length adjusting module are fixed on two sides of the mechanical support corresponding to the light splitting device, and the microscope objective is used for reflecting reflected light or fluorescence of the section sample to the lens group and the imaging module for imaging.

The imaging module shapes and transmits light beams of the light source to the light splitting device, and the light beams are reflected by the light splitting device and then are irradiated on the slice sample through the plurality of microobjectives; reflected light or excited fluorescence on the sliced sample is collected through the microscope objective, and the reflected light or the excited fluorescence is reflected by the same light splitter and enters the imaging module through the lens group, so that multi-channel imaging is realized.

The objective lens focal length adjusting module and the microscope objective lens are cascaded together and then are fixed on the mechanical support together, and the microscope objective lens focal length adjusting module can be any linear motion module, controls the distance between the microscope objective lens and a sample and adjusts the definition of microscopic imaging.

Furthermore, the light source adopts a wide-field light source to realize wide-field imaging of the sliced sample, and can also be a laser light source with a certain specific wavelength or a plurality of laser light sources with specific wavelengths to realize imaging of different fluorescence bands of the sliced sample by matching with a film system on the surface of the light splitting device.

Further, the light splitting device is a polygonal pyramid-shaped or polygonal frustum-shaped structure, each of the side surfaces of which has an optical interface for reflecting light from the light source and irradiating it onto the sliced sample through the microscope objective.

The side surface of the light splitting device is plated with a specific film system, so that light emitted by a light source is reflected to different directions through the light beam after shaping transmission, and is irradiated on a slice sample after passing through the microscope objective, and meanwhile, reflected light or fluorescence received by the microscope objective is reflected to the lens group and the imaging module for imaging.

The number of the side faces of the light splitting device corresponds to that of the imaging channels, the amplification of the imaging channels of the system is realized by increasing the volume of the light splitting device or increasing the number of the side faces and matching more imaging channels, more channels image samples at the same time, and the flux of the pathological section imaging device is improved.

Further, the optical interface adopts a half-transmitting and half-reflecting mirror or a dichroic mirror.

Furthermore, the light source, the light beam shaping module, the light splitting device, the lens group and the imaging module are positioned on the same straight line.

Furthermore, the slice samples are parallelly arranged on two sides of the motion module, and the motion module can drive the whole scanning imaging device to move along the parallel plane of the slice samples, so that the scanning imaging of a plurality of slice samples is realized simultaneously; or the slice samples are distributed annularly around the central shaft of the mechanical support, the motion module drives the scanning imaging device to rotate around the central shaft of the mechanical support, the axial motion is superposed to form spiral motion, the simultaneous scanning of different slice samples is realized, and the distortion correction and splicing treatment are subsequently carried out on the images.

Further, the lens group adopts a plurality of lens combinations or is an integrated lens group.

Furthermore, the imaging module is formed by combining a plurality of photodetectors, and an integral large-area photodetector can be selected.

According to the invention, through the arranged light splitting device with the polygonal pyramid or polygonal frustum structure, the side surface is provided with the optical interface, the number of the side surfaces corresponds to that of the imaging channels, the amplification of the imaging channels of the system is realized by increasing the volume of the light splitting device or increasing the number of the side surfaces and matching more imaging channels, the sample is imaged through more imaging channels at the same time, the flux of the imaging device for slicing the sample is improved, the time for finishing the digital scanning imaging of a large number of slices is shortened, and the working efficiency is improved.

Drawings

FIG. 1 is a schematic view of a yz plane scanning imaging device;

FIG. 2 is a schematic structural diagram of a pathological section scanning imaging device with multiple light sources;

FIG. 3 is a schematic view of a rotational + z-axis linear scanning imaging device of an imaging assembly;

FIG. 4 is a schematic structural diagram of a four-channel pathological section scanning imaging device;

FIG. 5 is a schematic view of the overall structure of the present invention;

in the figure, 1-mechanical support, 2-light source, 3-beam shaping module, 4-spectroscope, 5-lens group, 6-imaging module, 7-motion module, 8-microscope objective, 9-objective focal length adjusting module, 10-slice sample and 11-optical interface.

Detailed Description

The invention is described in further detail below with reference to the accompanying drawings in order to better understand the technical solution.

As shown in fig. 1-5, the high-throughput pathological section scanning imaging device of the present invention mainly includes a light source 2, a beam shaping module 3, a light splitter 4, a lens group 5, and an imaging module 6, which are sequentially and fixedly disposed on a mechanical support 1, and are disposed on the same straight line, wherein the mechanical support 2 and a motion module 7 are cascaded together, the motion module 7 drives the whole mechanical support 2 to move, a microscope objective 8 and an objective focal length adjusting module 9 are fixed on two sides of the mechanical support 2 corresponding to the light splitter 4, and the microscope objective 8 is used for reflecting reflected light or fluorescence of a slice sample 10 to the lens group 5 and the imaging module 6 for imaging.

The light source 2 adopts a wide-field light source or a laser light source, the wide-field light source realizes wide-field imaging of the sliced sample 10, and the laser light source realizes imaging of different fluorescence bands of the sliced sample 10; the light beam shaping module 3 is mainly an optical lens group and mainly used for shaping and transmitting light beams of a light source; the light splitting device 4 is made of special materials, an optical interface 11 arranged on the light splitting device can be a half-transmitting and half-reflecting mirror or a dichroic mirror, and the main function is to reflect light of a light source and irradiate the light on a slice sample 10 through a microscope objective 8; the light-splitting device 4 is a polygonal pyramid or a polygonal frustum structure, the side surface of the light-splitting device is provided with an optical interface 11, the optical interface 11 is used for reflecting light of a light source and irradiating the light on a slice sample 10 through a microscope objective 8, and the optical interface 11 adopts a semi-transparent and semi-reflective mirror or a dichroic mirror; the lens group 5 adopts a plurality of lens combinations or is an integrated lens group; the imaging module 6 is formed by combining a plurality of photodetectors or can be an integrated photodetector.

The slice sample 10 is placed on two sides of the motion module 7 in parallel or distributed annularly around the central axis of the mechanical support 1, the reflected light or the excited fluorescence from the surface of the slice sample 10 irradiates on the optical interface 11 of the light splitter 4 through the microscope objective 8, and is received by the imaging module 6 for imaging after being reflected through the lens group 5; the imaging module 6 is a single or a combination of a plurality of imaging devices. The objective focal length adjusting module 9 is a linear motion device, and is used for adjusting the distance between the microscope objective 8 and the slice sample 10 and adjusting the definition of microscopic imaging.

Fig. 1 is a yz-direction planar scanning imaging device, fig. 2 is a scanning imaging device for pathological sections with multiple light sources, fig. 3 is a device in which a motion module drives an imaging component to rotate and simultaneously scans an imaging device linearly along a z-axis, fig. 4 is a four-channel pathological section scanning imaging device, and fig. 5 is a schematic three-dimensional structure diagram of the device.