阅读说明：本技术 阿苯达唑亚砜在制备防治柑橘采后绿霉菌的农药杀菌剂的应用 (Application of albendazole sulfoxide in preparation of pesticide bactericide for preventing and treating citrus postharvest chloromycete ) 是由 彭程 邱继水 朱从一 陆育生 常晓晓 于 2021-05-07 设计创作，主要内容包括：本发明首次公开了式I所示的阿苯达唑亚砜在制备防治柑橘采后绿霉菌的农药杀菌剂的应用：,在防治柑橘采后绿霉菌引起的农作物病害,安全性显著高于商品化杀菌剂咪鲜胺。(The invention discloses the application of albendazole sulfoxide shown in a formula I in preparing a pesticide bactericide for preventing and treating citrus postharvest chloromycete for the first time: the safety of the bactericide is obviously higher than that of a commercial bactericide prochloraz when the bactericide is used for preventing and treating crop diseases caused by pseudomonas aeruginosa after citrus harvest.)

1. The application of albendazole sulfoxide shown in formula 1 in preparing pesticide bactericide for preventing and treating citrus postharvest chloromycete is as follows:

2. the application of the albendazole sulfoxide to the preparation of the pesticide bactericide for preventing and treating citrus postharvest chloromycete as claimed in claim 1, wherein the concentration of the albendazole sulfoxide is 1-10 μ g/mL in use.

3. The use of albendazole sulfoxide according to claim 1 or 2 for preparing a pesticide bactericide for controlling citrus postharvest chloromycete, wherein the pathogenic bacterium of citrus postharvest chloromycete is Penicillium digitatum.

The technical field is as follows:

the invention relates to application of albendazole sulfoxide in preparation of a pesticide bactericide for preventing and treating citrus postharvest chloromycete.

Background art:

the citrus belongs to the genus Citrus of the family Rutaceae, the citrus in China is mainly sold fresh, the maturation period is basically concentrated in 9-11 months, the production area and the consumption area of the citrus have a certain distance, the maturation period is relatively concentrated, a certain storage and transportation period is required to adjust the contradiction of market supply and demand, and during the period, fruit loss caused by microbial infection occurs in a large amount, such as rotting, nutrition and water loss frequently occur, and great economic loss is caused. Among them, fungal diseases are most serious, and common fungal diseases include green mold (Penicillium digitatum), Penicillium disease (p. italicum), acid rot (oosporitrialeurotii), black stalk rot (Diplodia natalensis), brown stalk rot (Phomopsis cytoplasma), black rot (Alternaria citri), anthracnose (colletotrichoides), brown rot (phohthora citrophthora), and the like. In recent years, it has been found that 30% to 50% of postharvest rot of citrus in our country is caused by green mold (p. Therefore, the storage and preservation of citrus is the direction and hot spot of domestic and foreign development and research in the coming years.

Although breeding resistant varieties is the most economical and environmentally friendly method for controlling the diseases, the use of chemical agents in the case of lack of resistant varieties or incomplete resistance is still the main means for controlling fungal diseases of crops. Albendazole sulfoxide can be used for expelling ascaris, pinworm, tapeworm, whipworm, hookworm, strongyloides stercoralis and the like in medicine. However, at present, no related research on the resistance of albendazole sulfoxide to the postharvest pseudomonas aeruginosa disease of citrus is available.

The invention content is as follows:

the invention aims to provide application of albendazole sulfoxide in preparation of a pesticide bactericide for preventing and treating citrus postharvest chloromycete.

The invention is realized by the following technical scheme:

the application of albendazole sulfoxide shown as a formula I in preparing a pesticide bactericide for preventing and treating citrus postharvest chloromycete is as follows:

the albendazole sulfoxide concentration is 1-10 μ g/mL when in use.

The pathogenic bacteria of the postharvest chlorophycea citrulli is Penicillium digitatum.

The invention has the following beneficial effects: the invention discovers the new application of albendazole sulfoxide as a pesticide bactericide in preventing and controlling citrus postharvest chloromycete for the first time. The pesticide composition can be used for preventing and treating crop diseases caused by the pathogenic bacteria Penicillium digitatum of the postharvest chlorophycea citri after citrus harvest, and the safety is obviously higher than that of the commercial fungicide prochloraz.

Description of the drawings:

FIG. 1 shows the inhibition of post-harvest Pythium species in citrus (day six).

FIG. 2 shows the inhibitory effect of albendazole sulfoxide at different concentrations on P.aeruginosa in citrus after harvest (6 days).

FIG. 3 shows the 7-day green mold growth of the fruit of Or Citrus aurantiaca when inoculated with the spores of Chloromyces fragrans.

The specific implementation mode is as follows:

the following is a further description of the invention and is not intended to be limiting.

Example 1: inhibition effect of albendazole sulfoxide on citrus postharvest chloromycete

1) Test drug

Albendazole sulfoxide, prochloraz (positive control compound, commercial pesticide), terbinafine.

(2) Test fungal species

The number of the tested fungal strains is 1, and the fungal strains are Penicillium digitatum which is a pathogen of the citrus greening mycosis.

(3) Determination of anti-plant-fungal Activity

Preparation of test bacterium plates: under aseptic condition, respectively inoculating pure test bacteria to a sterile PDA culture medium plate by a dense wave line drawing method, culturing at 27 deg.C for about one week, and allowing the plate to grow bacterial lawn.

Preparation of the culture medium and sterilization thereof: the culture medium is PDA solid culture medium, and the formula is as follows: 200g of potato, 20g of glucose, 20g of agar and 1000mL of tap water. The preparation process comprises the following steps: cutting peeled potato into 5mm square pieces, adding tap water, boiling for 20min, filtering with 8 layers of gauze, adding corresponding amount of glucose and agar powder into the filtrate, heating and stirring to dissolve, adding tap water to desired volume, packaging into 250mL triangular bottles, sealing (each bottle contains 100mL culture medium), and sterilizing with autoclave at 115 deg.C for 30 min.

Preparing a liquid medicine: weighing 1.00mg of each of the compounds of prochloraz, albendazole sulfoxide and terbinafine in a 1.5mL sterile centrifuge tube, and respectively adding 1mL of DMSO for dissolving.

Preparation of a culture medium with medicine: heating and melting a culture medium in a sterilized triangular flask, cooling to about 50 ℃, respectively adding the prepared liquid medicine on a clean bench through aseptic operation, slightly shaking the triangular flask to uniformly distribute the liquid medicine in the culture medium, preparing albendazole sulfoxide into a culture medium with medicine with a final concentration of 10 mu g/mL, immediately pouring the culture medium with medicine into 5 sterile culture dishes (20 mL of culture medium in each culture dish) while the culture medium is hot to prepare flat plates with uniform thickness, and marking, wherein prochloraz is a positive control; in the same procedure, plates of negative control DMSO (0.1mL directly in 100mL sterile medium) were prepared and labeled.

And (3) testing the bacteriostatic rate: under the aseptic condition, punching a certain amount of fungus cakes on a test fungus plate by using an aseptic puncher, taking the fungus cakes by using an inoculating ring holder, placing the fungus cakes on a prepared culture medium with medicine, enabling the hyphae to face downwards, marking 1 fungus cake per dish (three times), placing the fungus cakes in a thermostat at 27 ℃ for culturing for 72 hours, taking out a culture, measuring the diameter of a bacterial colony by using a caliper (measuring the cross quantity twice, measuring all three repeated plates, taking the average number of the plates), calculating the bacteriostasis rate according to the formula (1), and obtaining the calculation result of the activity of the albendazole sulfoxide against plant fungi at the concentration of 10 mu g/mL in the table 1.

Inhibition rate (negative control growth diameter-treated growth diameter)/(negative control growth diameter-5) × 100% … (1).

As can be seen from Table 1 and figure 1, the albendazole sulfoxide compound has a good sterilization effect on citrus postharvest chloromycete, wherein the concentration of the albendazole sulfoxide compound is 10 mu g/mL and can reach 100%.

TABLE 1 Albendazole sulfoxide and Prochloraz of the Compounds for the inhibition of plant fungi

Compound (I)	Bacteriostatic ratio (%)
		Prochloraz	100
Albendazole sulfoxide	100
		Terbinafine	43.91

Example 2: inhibition effect of albendazole sulfoxide with different concentrations on citrus postharvest chloromycete

Reference example 1, except that the preparation of the medium with drug: heating and melting a culture medium in a sterilized triangular flask, cooling to about 50 ℃, respectively adding the prepared liquid medicine on a clean bench through aseptic operation, slightly shaking the triangular flask to uniformly distribute the liquid medicine in the culture medium, preparing the albendazole sulfoxide into culture mediums with the final concentrations of 0.5 mu g/mL, 1 mu g/mL, 2 mu g/mL, 4 mu g/mL and 8 mu g/mL respectively, immediately pouring the media into 5 sterile culture dishes (20 mL of culture medium in each culture dish) while the media are hot, preparing flat plates with uniform thickness, and marking; in the same procedure, plates of negative control DMSO (0.1mL directly in 100mL sterile medium) were prepared and labeled.

The results of the calculation of the inhibitory activity of albendazole sulfoxide at different concentrations on citrus postharvest chloromycete are shown in table 2.

TABLE 2 inhibition of post-harvest Chloromyces in citrus by Albendazole sulfoxide at various concentrations (6 days)

The virulence equation is: 6.7264x +2.4537, coefficient of correlation R²＝0.9917，EC₅₀＝2.3908μg/ml

Example 3: inhibition effect of albendazole sulfoxide with different concentrations on citrus postharvest chloromycete

(1) Test drug

Albendazole sulfoxide (positive control compound, commercial pesticide).

(2) Test material

And (5) processing citrus.

(3) Inhibition effect of albendazole sulfoxide on pseudomonas aeruginosa of sugar orange after harvest

Preparing a liquid medicine: 1.00mg of albendazole sulfoxide is weighed into a 1.5mL sterile centrifuge tube, and 1mL of acetic acid is added to dissolve the albendazole sulfoxide.

And (3) testing the bacteriostatic rate: randomly dividing purchased Or into 2 groups after damaged fruits are removed, wherein each group contains more than or equal to 20 fruits. Each fruit is inoculated with 20 mul of albendazole sulfoxide with the concentration of 5 mul/ml, and after the medicament is completely absorbed, 5 mul of albendazole sulfoxide with the concentration of 1 multiplied by 10 is inoculated⁶And (4) spores. Air dried, and the results after seven days are shown in fig. 3.

As is clear from Table 2, albendazole sulfoxide of the present invention showed excellent inhibitory action against Pseudomonas aeruginosa after Citrus harvesting, and its EC₅₀The average value was 2.3908. mu.g/mL. In conclusion, the albendazole sulfoxide disclosed by the invention has a remarkable inhibitory activity on the citrus post-harvest pseudomonas aeruginosa.