阅读说明：本技术 一种幼犬营养膏及其制备方法和应用 (Nutrient paste for puppies and preparation method and application thereof ) 是由 张泽宇 吴怡 胡金华 彭晓培 郑美大 夏黎明 马曦 马东立 于 2020-05-11 设计创作，主要内容包括：本发明提供了一种幼犬营养膏及其制备方法和应用。具体地说,所述幼犬营养膏包含如下组分或由如下组分组成：功能卵磷脂、蛋白质、风味诱食料、氨基酸类组分、维生素、矿物质、有机酸、油脂、功能寡糖、载体、着色剂、增稠剂和水。本发明还提供了一种幼犬营养膏在幼犬饲喂中的应用以及制备所述营养膏的制备方法。本发明能够显著促进幼犬的大脑发育和身体的生长发育,避免发生过敏反应,有利于毛发健康,促进消化吸收,避免因乳糖不耐受导致的腹泻问题,并提高幼犬食欲。(The invention provides a nutrient paste for puppies and a preparation method and application thereof. Specifically, the puppy nutrition paste comprises or consists of the following components: functional lecithin, protein, flavor inducing materials, amino acid components, vitamins, minerals, organic acids, grease, functional oligosaccharide, a carrier, a coloring agent, a thickening agent and water. The invention also provides application of the puppy nutrition paste in puppy feeding and a preparation method for preparing the nutrition paste. The feed additive can obviously promote the brain development and the body growth development of puppies, avoid anaphylactic reaction, is beneficial to hair health, promotes digestion and absorption, avoids the problem of diarrhea caused by lactose intolerance, and improves the appetite of the puppies.)

1. A nutritional paste for puppies, wherein the nutritional paste comprises or consists of the following components: functional lecithin, protein, flavor inducing materials, amino acid components, vitamins, minerals, organic acids, grease, functional oligosaccharide, a carrier, a coloring agent, a thickening agent and water.

2. The nutritional paste of claim 1, wherein:

the nutritional cream includes 11.5 to 19.5 parts by weight of functional lecithin, 4.0 to 14 parts by weight of protein, 2.4 to 9.5 parts by weight of flavor inducer material, 1.1 to 4.4 parts by weight of amino acid component, 0.042 to 0.305 parts by weight of vitamin, 0.4 to 3 parts by weight of mineral, 0.01 to 0.5 parts by weight of organic acid, 1.1 to 9 parts by weight of fat and oil, 0.003 to 0.05 parts by weight of functional oligosaccharide, 35 to 55 parts by weight of carrier, 0.1 to 0.6 parts by weight of colorant, 0.5 to 1.5 parts by weight of thickener, and 8 to 24 parts by weight of water.

3. The nutritional paste of claim 1 or 2, wherein:

the functional lecithin comprises one or more components selected from the group consisting of egg yolk powder and soybean lecithin;

the protein comprises one or more selected from the group consisting of chicken protein powder and chicken meal;

the flavor attractant comprises one or more selected from the group consisting of chicken liver powder and chicken plasma;

the amino acid component comprises one or more selected from the group consisting of taurine, cystine, and lysine;

the vitamins comprise one or more selected from the group consisting of vitamin B1, vitamin B2, vitamin B12, and vitamin E;

the mineral comprises dibasic calcium phosphate;

the organic acid comprises lactic acid;

the fats and oils comprise one or more selected from the group consisting of deep sea fish oil and linseed oil;

the fructooligosaccharide comprises fructooligosaccharide;

the carrier comprises maltose syrup;

the colorant comprises caramel color; and/or

The thickener comprises sodium carboxymethylcellulose.

4. The nutritional paste according to any one of claims 1 to 3, wherein:

the functional lecithin is one or more components selected from the group consisting of egg yolk powder and soybean lecithin;

the protein is selected from one or more of the group consisting of chicken protein powder and chicken meal;

the flavor attractant is selected from one or more of the group consisting of chicken liver powder and chicken soluble paste;

the amino acid component is selected from one or more of the group consisting of taurine, cystine and lysine;

the vitamins are selected from one or more of the group consisting of vitamin B1, vitamin B2, vitamin B12 and vitamin E;

the mineral substance is calcium hydrophosphate;

the organic acid is lactic acid;

the grease is selected from one or more of the group consisting of deep sea fish oil and linseed oil;

the fructo-oligosaccharide is fructo-oligosaccharide;

the carrier is maltose syrup;

the colorant is caramel color; and/or

The thickening agent is sodium carboxymethyl cellulose.

5. The nutritional paste according to any one of claims 1 to 4, wherein:

the functional lecithin is composed of egg yolk powder and soybean lecithin;

the protein consists of chicken protein powder and chicken powder;

the flavor inducing food is composed of chicken liver powder and chicken soluble paste;

the amino acid component consists of taurine, cystine and lysine;

the vitamins consist of vitamin B1, vitamin B2, vitamin B12 and vitamin E;

the mineral substance is calcium hydrophosphate;

the organic acid is lactic acid;

the grease consists of deep sea fish oil and linseed oil;

the fructo-oligosaccharide is fructo-oligosaccharide;

the carrier is maltose syrup;

the colorant is caramel color; and/or

The thickening agent is sodium carboxymethyl cellulose.

6. The nutritional paste according to any one of claims 1 to 5, wherein:

when present, the content of each component is as follows in parts by weight:

7. the nutritional paste according to any one of claims 1 to 6, wherein:

the puppy nutritional cream further comprises a preservative and/or a flavoring agent; preferably, the preservative is potassium sorbate, and/or the flavoring agent is sodium chloride; more preferably, the puppy nutrition paste further comprises 0 to 0.2 parts by weight of potassium sorbate and/or 0 to 2 parts by weight of sodium chloride, and further preferably further comprises 0.1 to 0.2 parts by weight of potassium sorbate and/or 0.1 to 2 parts by weight of sodium chloride.

8. The nutritional paste according to any one of claims 1 to 7, wherein:

the puppy nutritional cream comprises one or more selected from the group consisting of coated nano zinc oxide, hawthorn extract and goat milk powder; preferably, the puppy nutrition paste comprises 0.005 to 0.03 weight part of coated nano zinc oxide, 0.01 to 0.1 weight part of hawthorn extract and 0.5 to 3.5 weight parts of goat milk powder.

9. Use of a puppy nutritional cream in the feeding of a puppy or in the preparation of a feed for a puppy, wherein:

the puppy nutritional cream comprises 0 to 0.03 parts by weight of coated nano zinc oxide, 0 to 0.1 parts by weight of hawthorn extract and 0 to 3.5 parts by weight of goat milk powder, and the puppy nutritional cream comprises at least one, at least two or all three selected from the group consisting of coated nano zinc oxide, hawthorn extract and goat milk powder; preferably, the puppy nutrition paste comprises 0.005 to 0.03 weight part of coated nano zinc oxide, 0.01 to 0.1 weight part of hawthorn extract and 0.5 to 3.5 weight parts of goat milk powder; or

The puppy nutritional cream of any one of claims 1 to 8.

10. A process for preparing a puppy nutritional paste according to any one of claims 1 to 8, wherein the process comprises the steps of:

(1) mixing the carrier, the functional oligosaccharide and water to prepare carrier syrup;

(2) adding optional fructus crataegi extract, yolk powder, protein, flavor inducing material and optional antiseptic, stirring and homogenizing to obtain mixture; in addition, homogenizing, stirring and heating the deep sea fish oil and the linseed oil to 75-80 ℃ to obtain mixed oil;

(3) adding optional soybean lecithin and thickener into the mixture, homogenizing and emulsifying, slowly dropping the mixed oil for three times, homogenizing and emulsifying to obtain a first emulsion;

(4) adding organic acid, mineral substances, sodium chloride, amino acid components, vitamin components, optional coating nano zinc oxide and optional goat milk powder into the first emulsion, uniformly mixing, adding a colorant for color mixing, stirring and emulsifying to obtain a second emulsion, and cooling to room temperature to form a paste serving as a nutritional paste;

preferably, the method further comprises the steps of: (5) sterilizing the paste by a magnetic sterilization method; and (6) filling and sealing the sterilized paste, and performing secondary sterilization to obtain the nutritional paste product.

Technical Field

The invention relates to the technical field of pet food preparations, in particular to a nutrient paste for puppies and a preparation method and application thereof.

Background

With the improvement of living standard and the development of society, more and more families are raising pets, especially pet dogs. According to the American Pet Products Association (APPA), nearly three quarters of American domestic pets are willing to spend a large amount of time and money for the young partners of the hairy antler to be happy. The pet industry has grown 2 times over the past 15 years and pet food has rapidly become a popular culture. How to raise the pet is a general concern for pet raising families.

For the young, postpartum, postoperative,The dog with weak body can rapidly supplement nutrition required by body, enhance immunity, and helpDogThe weak body is quickly recovered, and the nutrition needs to be supplemented frequently. The means of supplementing the nutrition may be in the form of a nutritional supplement. At present, the nutritional supplement usually adopts ointment for supplementing nutrition for pets, and is mainly used for health conditioning of pets, supplementing insufficient nutrition, preventing diseases and relieving clinical discomfort symptoms of pets.

For young dogs, gastrointestinal function is not well established and resistance is poor. Special attention is paid to diet conditions, namely balanced nutrition and enough nutrient substances. If the young dog is overerased, the stomach is easily injured, and the gastrointestinal function is affected. If the food is too little, the nutrient substances necessary for the growth and development of the organism can not be ensured, so that the immunity resistance is poor and the disease is easy to occur. Thus, puppies are in great need of additional high concentration nutritional supplements.

The current nutrient paste for puppies mainly has one or more of the following problems: the young dog is not like eating, and the problems of insufficient nutrition, insufficient development assistance to the body and the brain, anaphylactic reaction, poor digestion and absorption, easy diarrhea, poor fur and the like are solved.

Disclosure of Invention

In order to solve one or more of the above technical problems in the prior art, the present invention provides, in a first aspect, a puppy nutrition paste, wherein the nutrition paste comprises or consists of the following components: functional lecithin, protein, flavor inducing materials, amino acid components, vitamins, minerals, organic acids, grease, functional oligosaccharide, a carrier, a coloring agent, a thickening agent and water.

The invention provides in a second aspect the use of a puppy nutrition cream comprising from 0 to 0.03 parts by weight of coated nano zinc oxide, from 0 to 0.1 parts by weight of hawthorn extract and from 0 to 3.5 parts by weight of goat milk powder, and the puppy nutrition cream comprising at least one, at least two or all three selected from the group consisting of coated nano zinc oxide, hawthorn extract and goat milk powder, for feeding to or preparing a feed for a puppy.

The present invention also provides in a third aspect a method of preparing a nutritional paste according to the first aspect of the invention, the method comprising:

(1) mixing the carrier, the functional oligosaccharide and water to prepare carrier syrup;

(2) adding optional fructus crataegi extract, yolk powder, protein, flavor inducing material and optional antiseptic, stirring and homogenizing to obtain mixture; in addition, homogenizing, stirring and heating the deep sea fish oil and the linseed oil to 75-80 ℃ to obtain mixed oil;

(3) adding optional soybean lecithin and thickener into the mixture, homogenizing and emulsifying, slowly dropping the mixed oil for three times, homogenizing and emulsifying to obtain a first emulsion;

(4) adding organic acid, mineral substances, sodium chloride, amino acid components, vitamin components, optional coating nano zinc oxide and optional goat milk powder into the first emulsion, uniformly mixing, adding a coloring agent for color mixing, stirring and emulsifying to obtain a second emulsion, and cooling to room temperature to form a paste serving as the nutritional paste.

Compared with the prior art, the invention has the following technical advantages: the brain development and the body growth development of the puppy can be remarkably promoted; the anaphylactic reaction is avoided; is beneficial to the health of hair; the digestive absorption rate is high, and the problem of diarrhea caused by lactose intolerance is avoided; improve the appetite of puppies, and the like.

Drawings

Figure 1 is a selection of puppy nutritional cream taste tests.

Figure 2 is a graph of the effect of puppy nutrition cream on test dog body weight.

Figure 3 is a graph of the effect of puppy nutrition cream on the average daily gain of test dogs.

Figure 4 is a graph of the effect of puppy nutritional cream on average daily food intake of test dogs.

FIG. 5-A is a graph of the effect of puppy nutrition cream on the coat status of test dogs.

FIG. 5-B is a graph of the effect of puppy nutrition cream on the coat status of test dogs.

FIG. 5-C is the effect of puppy nutrition cream on the coat status of test dogs.

Detailed Description

In order to solve one or more of the above technical problems in the prior art, the present invention provides, in a first aspect, a puppy nutrition paste, wherein the nutrition paste comprises or consists of the following components: functional lecithin, protein, flavor inducing materials, amino acid components, vitamins, minerals, organic acids, grease, functional oligosaccharide, a carrier, a coloring agent, a thickening agent and water.

In some preferred embodiments, the nutritional cream comprises 11.5 to 19.5 parts by weight (e.g., 12, 13, 14, 15, 16, 17, or 18 parts by weight) of functional lecithin, 4.0 to 14 parts by weight (e.g., 6, 8, 10, or 12 parts by weight) of protein, 2.4 to 9.5 parts by weight (e.g., 3, 5, or 7 parts by weight) of flavor-inducing material, 1.1 to 4.4 parts by weight (e.g., 2, 3, or 4 parts by weight) of amino acid component, 0.042 to 0.305 parts by weight (e.g., 0.05, 0.1, 0.2, or 0.3 parts by weight) of vitamin, 0.4 to 3 parts by weight (e.g., 0.5, 1.0, or 2.0 parts by weight) of mineral, 0.01 to 0.5 parts by weight (e.01, 0.02, or 0.5 parts by weight) of organic acid, 1.1 to 9 parts by weight (e.2, 4, 6, or 8 parts by weight) of mineral, 0.01 to 0.02 parts by weight) of functional oligosaccharide, 0.04 parts by weight (e.05 parts by weight) of carrier, 0.02 to 9 parts by weight) of functional lecithin, 0.35 parts by weight (e.05 parts by weight) of carrier, 0.3 parts by weight) of functional oligosaccharide, 0.3 parts by weight, or 3 parts by weight of functional oligosaccharide, or 0.0.3 parts by weight of carrier, or 6 parts by weight of functional lecithin, 0.1 to 0.6 parts by weight (e.g., 0.1, 0.3, or 0.6 parts by weight) of a colorant, 0.5 to 1.5 parts by weight (e.g., 0.5, 1.0, or 1.5 parts by weight) of a thickener, and 8 to 24 parts by weight (e.g., 10, 15, or 20 parts by weight) of water.

In other preferred embodiments, the functional lecithin comprises one or more components selected from the group consisting of egg yolk powder and soy lecithin; the protein comprises one or more selected from the group consisting of chicken protein powder and chicken meal; the flavor attractant comprises one or more selected from the group consisting of chicken liver powder and chicken plasma; the amino acid component comprises one or more selected from the group consisting of taurine, cystine, and lysine; the vitamins comprise one or more selected from the group consisting of vitamin B1, vitamin B2, vitamin B12, and vitamin E; the mineral comprises dibasic calcium phosphate; the organic acid comprises lactic acid; the fats and oils comprise one or more selected from the group consisting of deep sea fish oil and linseed oil; the fructooligosaccharide comprises fructooligosaccharide; the carrier comprises maltose syrup; the colorant comprises caramel color; and/or the thickener comprises sodium carboxymethylcellulose.

In other preferred embodiments, the functional lecithin is selected from one or more components of the group consisting of egg yolk powder and soy lecithin; the protein is selected from one or more of the group consisting of chicken protein powder and chicken meal;

the flavor attractant is selected from one or more of the group consisting of chicken liver powder and chicken soluble paste; the amino acid component is selected from one or more of the group consisting of taurine, cystine and lysine; the vitamins are selected from one or more of the group consisting of vitamin B1, vitamin B2, vitamin B12 and vitamin E; the mineral substance is calcium hydrophosphate; the organic acid is lactic acid; the grease is selected from one or more of the group consisting of deep sea fish oil and linseed oil; the fructo-oligosaccharide is fructo-oligosaccharide; the carrier is maltose syrup; the colorant is caramel color; and/or the thickener is sodium carboxymethylcellulose.

In other preferred embodiments, the functional lecithin is comprised of egg yolk powder and soy lecithin; the protein consists of chicken protein powder and chicken powder; the flavor inducing food is composed of chicken liver powder and chicken soluble paste; the amino acid component consists of taurine, cystine and lysine; the vitamins consist of vitamin B1, vitamin B2, vitamin B12 and vitamin E; the mineral substance is calcium hydrophosphate; the organic acid is lactic acid; the grease consists of deep sea fish oil and linseed oil; the fructo-oligosaccharide is fructo-oligosaccharide; the carrier is maltose syrup; the colorant is caramel color; and/or the thickener is sodium carboxymethylcellulose.

In other preferred embodiments, the amounts of the components, when present, in parts by weight are as follows:

in other preferred embodiments, the puppy nutritional cream further comprises a preservative and/or a flavoring agent; preferably, the preservative is potassium sorbate, and/or the flavoring agent is sodium chloride; more preferably, the puppy nutrition paste further comprises 0 to 0.2 parts by weight of potassium sorbate and/or 0 to 2 parts by weight of sodium chloride, and further preferably further comprises 0.1 to 0.2 parts by weight of potassium sorbate and/or 0.1 to 2 parts by weight of sodium chloride.

In other preferred embodiments, the puppy nutritional cream comprises one or more selected from the group consisting of coated nano zinc oxide, hawthorn extract, and goat milk powder; preferably, the puppy nutrition paste comprises 0.005 to 0.03 weight part of coated nano zinc oxide, 0.01 to 0.1 weight part of hawthorn extract and 0.5 to 3.5 weight parts of goat milk powder.

The feeding method of the nutrient paste is not particularly limited, and can be carried out in the following way: (1) direct smearing: can be applied to nose or mouth of dog for licking. (2) Feeding the daily ration: can be mixed with daily ration for feeding, and the addition amount in the complete pet food is not more than 10%. (3) Feeding in a dosing manner: the young dogs recovering or having poor appetite can be fed with appropriate dosage.

The feeding amount is not particularly limited in the present invention, but it is preferable to provide corresponding daily feeding amounts according to different body weights: 1-2 kg: about 1-3 g; 3-4 kg: about 4-6 g; 5-6 kg: about 7-8 g; 6kg above: about 8 g. The feeding amount can be adjusted according to the age, weight and activity of pets.

The invention provides in a second aspect the use of a puppy nutrition cream comprising from 0 to 0.03 parts by weight of coated nano zinc oxide, from 0 to 0.1 parts by weight of hawthorn extract and from 0 to 3.5 parts by weight of goat milk powder, and the puppy nutrition cream comprising at least one, at least two or all three selected from the group consisting of coated nano zinc oxide, hawthorn extract and goat milk powder, for feeding to or preparing a feed for a puppy. In some preferred embodiments, the puppy nutrition cream comprises 0.005 to 0.03 parts by weight of coated nano zinc oxide, 0.01 to 0.1 parts by weight of hawthorn extract, and 0.5 to 3.5 parts by weight of goat milk powder.

In other preferred embodiments, the puppy nutritional jelly is a puppy nutritional jelly according to the first aspect of the invention.

The present invention also provides in a third aspect a method of preparing a nutritional paste according to the first aspect of the invention, the method comprising:

(1) mixing the carrier, the functional oligosaccharide and water to prepare carrier syrup;

(2) adding optional fructus crataegi extract, yolk powder, protein, flavor inducing material and optional antiseptic, stirring and homogenizing to obtain mixture; in addition, homogenizing, stirring and heating the deep sea fish oil and the linseed oil to 75-80 ℃ to obtain mixed oil;

(3) adding optional soybean lecithin and thickener into the mixture, homogenizing and emulsifying, slowly dropping the mixed oil for three times, homogenizing and emulsifying to obtain a first emulsion;

(4) adding organic acid, mineral substances, sodium chloride, amino acid components, vitamin components, optional coating nano zinc oxide and optional goat milk powder into the first emulsion, uniformly mixing, adding a coloring agent for color mixing, stirring and emulsifying to obtain a second emulsion, and cooling to room temperature to form a paste serving as the nutritional paste.

Preferably, the method further comprises the steps of: (5) sterilizing the paste by a magnetic sterilization method; and (6) filling and sealing the sterilized paste, and performing secondary sterilization to obtain the nutritional paste product.

In the above method, if the nutritional paste to be prepared does not contain the corresponding component, the component is not added when referring to the component.

The paste can be prepared into 120 g/piece or a plurality of small packages, and the daily dose of each dog can be 6 g.

In some embodiments, the nutritional paste of the invention contains nano zinc oxide as a nano material, the particle size of the nano zinc oxide can be between 1 and 100nm, and the nano zinc oxide is different from common zinc oxide, so that the nutritional paste can meet the requirement of young animals on zinc under the condition of low addition amount, reduce the pollution of excrement and urine of the young animals to the environment, has the characteristics of high-efficiency biological activity, high absorption rate, strong oxidation resistance, safety, stability and the like, has the antibacterial and bacteriostatic effects, can be released at fixed points in the small intestine of the young dogs, optimizes the intestinal flora, and effectively relieves the stress caused by daily ration replacement after the young dogs are weaned. Moreover, the combination of the nano zinc oxide, the goat milk powder and the hawthorn extract, particularly the combination of the nano zinc oxide, the goat milk powder and the hawthorn extract, deep sea fish oil rich in EPA, DHA and the like, linseed oil, lecithin, B vitamins, cystine, lysine and the like can also promote the brain development of a puppy, avoid the allergy of a pet, contribute to hair health, promote the digestibility and avoid the diarrhea problem of the puppy caused by lactose intolerance.

Examples

The present invention will be further illustrated by the following examples, but the scope of the invention as claimed is not limited to these examples. The starting materials used in the examples are, unless otherwise indicated, conventional starting materials which are commercially available.

Preparation example

The following formula was used, 2kg for each nutritional cream:

the preparation method is carried out in the following way (if a certain component is not present, the use of the corresponding component is omitted):

(1) feeding in a first group: adding maltose syrup, fructo-oligosaccharide and water into an emulsifying pot. Starting a heating switch of the homogenizing emulsifying machine, and heating to 80 ℃ while stirring to prepare the carrier syrup.

(2) Feeding in a second group: adding fructus crataegi extract (Xianruin biotechnology limited), yolk powder, chicken protein powder, chicken liver powder, chicken slurry and potassium sorbate into an emulsifying pot, vacuumizing, and stirring for 15 min. Stirring, homogenizing, and slowly heating to 80 deg.C. Meanwhile, adding the deep sea fish oil and the linseed oil into an oil phase pot according to the formula proportion, vacuumizing, stirring, homogenizing, stirring and heating to 75-80 ℃.

(3) Third group of feeding + emulsification 1: adding soybean lecithin and sodium carboxymethylcellulose into the product obtained in the emulsifying pot according to the formula proportion, starting a homogenizing emulsifying machine, vacuumizing until the vacuum degree is 30-100 kPa, slowly dripping the mixed oil body in an oil phase pot into the emulsifying pot for three times until all oil phases are completely and uniformly coated by the water phase, and homogenizing and emulsifying for 4 minutes at 78 ℃ at the stirring speed of 60-300 r/min.

(4) Fourth group feeding + emulsification 2: the product obtained by the method is weighed according to the formula proportion, added with lactic acid, calcium hydrophosphate, sodium chloride, amino acid components (taurine, cystine and lysine), vitamin components (vitamin E, vitamin B1, vitamin B2 and vitamin B12), coating nano zinc oxide (Zhejiang Wanfang biological science and technology limited) and goat milk powder (Shanxi and pet food limited), and added with caramel for color matching, stirred at 78 ℃ for starting emulsification for 5-10 minutes, stopped for emulsification, continuously stirred for 30-40 minutes, and cooled to 45-50 ℃ while stirred.

(5) And (3) sterilization treatment: after the paste is cooled to normal temperature, the paste is sterilized by a magnetic sterilization method, and the magnetic strength is selected to be 0.6 tex.

(6) Filling: and transferring the sterilized paste to a filling line, and filling and sealing under the condition of opening a 2000-air-volume dynamic air sterilizer for 60 minutes (to avoid secondary pollution). The specification of the finished product is 120 g/count.

(7) And (3) sterilizing after filling: and (4) after filling, sterilizing by adopting a pasteurization method (at 70-80 ℃ for 30-40 minutes).

Nutritional pastes 1 to 7 were prepared according to the above formulations 1 to 7, respectively.

Test example 1

42 beagle dogs with the age of 4-6 months are selected in the test, and are divided into 7 groups according to the principle that the body weights are similar and the male half and the female half are respectively prepared into 1 to 7 nutrient pastes according to the formulas 1 to 7. The test period is 28 days, blood is collected after the test is finished, and antioxidant indexes (SOD and T-AOC) and immune indexes (IL-6) in blood plasma are detected. The blood sampling steps are as follows: (1) preparing tourniquet, dry cotton ball, alcohol cotton ball, blood sampling tube, and soft connection type blood sampling needle. (2) Animals were housed. (3) Animal hair was wetted with an alcohol cotton ball, and the wetted hair was separated to expose the approximate location of the blood vessel and disinfected. (4) And (5) tightening the tourniquet to find the blood vessel. (5) And (4) inserting the blood taking needle into the blood vessel, connecting the blood taking tube and loosening the tourniquet when blood flowing out of the blood collecting needle rubber tube is observed. (6) The blood taking needle is pulled out and stopped by a dry cotton ball. (7) The blood sample is sent for detection as soon as possible, and the shorter the storage time of the blood sample, the higher the accuracy. See table II for results.

TABLE II

The test results are shown in the following table. Compared with the nutrient pastes 1 to 4, the nutrient pastes 5 to 7 have higher antioxidant capacity, which is reflected in that the contents of antioxidant enzyme SOD and T-AOC in the blood plasma of dogs are obviously improved (P <0.05), and no obvious difference exists between the nutrient pastes 5 to 7 (P > 0.10). Compared with the nutrient pastes 1 to 4, the nutrient pastes 5 to 7 have better anti-inflammatory effect, which is reflected in that the content of the inflammatory factor IL-6 in the plasma of the dogs is obviously reduced (P <0.05), and no obvious difference exists between the nutrient pastes 5 to 7 (P > 0.10). In general, the nutritional paste 5 performed best.

Test example 2

First, test procedure

5kg of the nutrient pastes B3 and A3 were prepared in the same manner as in the above preparation examples, respectively, according to the formula 1 and the formula 5 in the above preparation examples.

Grouping condition, dosage and frequency of the nutrient paste:the experimental animals used in the examples were as follows: 15 beagle dogs of 4-6 months of age (initial body weight individual difference is within 1 kg). The test groups are divided into 3 groups according to the principle that the body weights are similar and the male and female halves are in each group. Group A is basic full-value dog food and A3 nutrition paste; group B is basic full-value dog food + B3 nutrition paste. Group C was a basal full-rate dog food + C3 control nutritional cream. Wherein the basic full-value dog food is ROYAL CANIN Royal dog food A3Preferably puppy dog food), the control nutritional cream was purchased as weishi nutritional cream. See table 1-1 below for details.

Tables 1 to 1: grouping condition, dosage and frequency of nutrient paste

The feeding mode and test time of the nutrient paste are as follows: the paste was squeezed into the dog mouth directly into the appropriate length using a hand-held nutrition paste packaging tube, daily at 8 am and 16 pm. The test period was 30 days.

The experimental contents are as follows:

15 beagle dogs were raised in a single cage. The body weight was weighed on days 0, 7, 14, 21, 30 at the start of the test. The night soil is regularly cleaned, the dog house is cleaned, the water basin is cleaned and the clean drinking water is replaced every morning. Recording the state of the excrement during the excrement cleaning. Feeding directly at 8 am every day. A3 nutrition paste 3g for each dog in group A, B3 nutrition paste 3g for each dog in group B, and C3 control nutrition paste 3g for each dog in group C. The dogs were fed 80g of basal full-rate dog food at 9 am. The water is freely drunk all day. 9 in the morning: the inspection is performed for 1 time 30 times, and the contents comprise photographing and recording the fur condition, the skin condition and the mental condition of each dog. The feed is directly fed at 16 pm, the A3 nutrient paste for each dog in group A, the B3 nutrient paste for each dog in group B, the C3 control nutrient paste for each dog in group C are 3g, and the basal full-rate dog food for each dog is fed after half an hour. Dogs were tethered out of the kennel at 17 o' clock each day for 1 hour of appropriate exercise. 7 at night: the dog was observed for 1 round 30 times and the mental state and the like of the dog were recorded. Feeding 140g of basic full-price dog food for each dog at 20 o' clock in the evening, removing the feed basin and weighing the rest feed in the feed basin when the dog turns for the first time and does not eat any more, calculating the feed intake of each dog in one day, and counting by 300g of feed intake in one day if the feed intake is finished completely.

And simultaneously carrying out comprehensive effect evaluation, carrying out product taste test 3 days before the test, and carrying out 8 o' clock once every morning for three times in total. 3 portions of 3g of each nutritional cream were prepared for each group, and 9 portions of the nutritional cream were randomly placed in the same parallel area with a 15 cm interval between each two plates. Each time 1 dog was led to an independent experimental area, the pet dogs were allowed to freely move to feed, and 15 dogs were individually selected. All the operations of the first 3 days are the same as the conventional operations except for the feeding mode of the nutrient paste.

Sampling indexes and methods:

(1) weight: a total of 5 body weights were recorded for each dog.

(2) Food intake: feed intake was recorded 1 total per dog daily.

(3) The situation of the quilt feathers: the condition of the hair of each dog was recorded 1 time by photographing every morning round, and the photograph required that the fullness and glossiness of the hair could be clearly seen.

(4) Skin condition: the skin state of each dog is recorded 1 time every morning round, and the hair on the back, chest, face (near the ear root) and the right neck of each dog is pulled out to observe the abnormal conditions of erythema, erythra, dandruff and the like.

(5) Defecation condition: feces were recorded for each dog 1 time daily in the morning at the time of feces clearance.

(6) Mental state: mental status was recorded 2 times daily for each dog on morning and evening tours.

(7) Selecting a record: each dog was pulled daily in the morning on the induction diet 3 days prior to the trial and the first selected nutrition was recorded. After the traction test was completed, the 8 extra pots were removed and the above routine recording was performed. Second, test results

Taste selection test for nutritional creams

As shown in table 2-1, the nutritional pastes of group a and group B were selected significantly more times in three independent taste tests than the positive control. In a total of 45 independent selections, the nutrition paste for the A3 puppy was selected 28 times with a ratio of 62.2%, the nutrition paste for the B3 puppy was selected 12 times with a ratio of 26.7%, while the nutrition paste for the positive control group for the commercial red dogs was selected only 5 times with a ratio of 11.1%, indicating that the nutrition paste for the A3 puppy has the best food calling property, and the nutrition paste for the B3 puppy has the second best nutrition paste for the commercial Weishi.

As shown in table 2-2 and fig. 1, the frequency of selection of the A3 puppy nutrition cream is significantly higher than that of the B3 puppy nutrition cream (P <0.01) and the commercially available weishi nutrition cream (P <0.01), and the frequency of selection of the B3 puppy nutrition cream and the commercially available red dog nutrition cream is also significantly higher than that of the commercially available weishi nutrition cream (P <0.01), which indicates that the food calling performance of both the A3 puppy nutrition cream and the B3 puppy nutrition cream is significantly better than that of the commercially available weishi nutrition cream. And the food calling performance of the A3 puppy nutrition paste is remarkably superior to that of the B3 puppy nutrition paste.

TABLE 2-1 puppy nutritional cream taste test selection results

	First taste test	Second taste test	Third taste test
				Number of times of selection of group A	8	10	10
Number of times of selection of group B	5	3	4
				Number of C group selections	2	2	1

Table 2-2 puppy nutrition paste taste test selection significance analysis

	Group A	Group B	Group C	P value
					Average number of selections (times)	9.33±0.67a	4.00±0.58b	1.67±0.33c	<0.01

Note: data are presented as mean ± standard deviation, with differences from the shoulder mark indicating significant differences (P < 0.05).

Test of comprehensive Effect

1. Body weight

As can be seen from tables 2-3 and FIG. 2, the weights of the three dogs gradually increased throughout the test period, which indicates that the A3 puppy nutrition cream, the B3 puppy nutrition cream and the commercially available Weishi nutrition cream have certain growth promoting effects on the test dogs.

As can be seen from tables 2-4 and fig. 3, the a3 juvenile nutritional cream of group a significantly increased the average daily gain (P <0.05) of the test dogs compared to group C during the test period, whereas the average daily gain of group B tended to be elevated but was not significant compared to group C. The results show that the growth promoting effect of the A3 puppy nutrition paste and the B3 puppy nutrition paste on test dogs is better than that of the commercial Weishi nutrition paste, and the effect of the A3 puppy nutrition paste is relatively better than that of the B3 puppy nutrition paste.

TABLE 2-3 weight record table for puppy nutrition paste test dog

TABLE 2-4 Effect of puppy nutritional pastes on average daily gain of test dogs

	Group A	Group B	Group C	P value
					Average daily gain (kg/d)	0.044±0.003a	0.037±0.003ab	0.033±0.004b	0.034

Note: data are presented as mean ± standard deviation, with differences from the shoulder mark indicating significant differences (P < 0.05).

2. Feed intake

As can be seen from tables 2-5 and fig. 4, the influence of the three groups of nutrient pastes on the average daily food intake of the test dogs in the test period has no significant difference, and 300g of the ration can be completely consumed by each young test dog in each test group in each test period without residue. The results show that the A3 puppy nutrition paste, the B3 puppy nutrition paste and the commercial Weishi nutrition paste have no influence on the feed intake.

TABLE 2-5 Effect of puppy Nutrition pastes on average daily food intake of test dogs

Note: data are presented as mean ± standard deviation, with differences from the shoulder mark indicating significant differences (P < 0.05).

3. Case of quilt fur

As shown in tables 2-6 and fig. 5, the coat condition of the three groups of test puppies was overall good over the test period. On test day 10, 1 dog in groups A and B had dull hair, while 2 dogs in group C had dull hair, accounting for 67% of group C. On day 15 of the test, 1 dog in each of group A and group B had dull and grey hair, while 2 dogs in group C had dull and grey hair, which continued until day 20 of the test disappeared. All dogs in each group had only slight alopecia from test day 1, which is normal seasonal alopecia in spring for beagle dogs, regardless of the test article. Dehairing occurred in all dogs of each group on test days 1, 5, 10, 15, 20. The depilation phenomenon was relieved in the later period of the experiment: on day 25 of the test, the dehairing phenomenon of 4 dogs in group a disappeared, and only 2 dogs were still slightly unhairing; the hair removal phenomenon of 5 dogs in group B disappeared, and only 1 dog still slightly removed hair; while only 1 dog in group C had no depilation and 2 had slight depilation. On day 30 of the test, the dehairing phenomenon disappeared in all dogs of group B, only 1 remained slightly depilated in group A, and 1 remained slightly depilated in group C. All dogs in the test group were more shiny than the initial hair. From the viewpoint of glossiness of the quilt hair and later depilation recovery, the effect of the nutrition cream of the two test groups on the quilt hair is slightly better than that of the commercially available Weishi nutrition cream.

TABLE 2-6 Effect of puppy Nutrition pastes on coat status of test dogs

4. Skin condition

As shown in tables 2-7, the skin condition of three groups of test puppies was overall good throughout the test period, with slight seasonal alopecia on the backs of all dogs in each group from day 1 of the test, with no apparent difference between groups. The depilation was relieved at the later stage of the test. All dogs have no abnormal conditions such as scurf, red rash, red swelling and the like in the whole test period, and the results show that the A3 nutrient cream and the B3 nutrient cream do not have adverse effects on the skin state of the test dogs, and the overall effects on the skin are not obviously different from those of the commercially available C3 Weishi control nutrient cream.

TABLE 2-7 Effect of puppy nutritional creams on skin status of test dogs

5. Fecal status

As shown in tables 2-8, during the test period, the feces of three groups of test puppies were mostly yellow soft feces and a few yellow brown hard feces, both of which were normal, and the dogs in groups A and B were free from diarrhea and other abnormal conditions. However, the phenomenon of defecation of 1 time appears on the 30 th day of the test in the group C, which indicates that neither the A3 puppy nutrition cream nor the B3 puppy nutrition cream can cause the digestion problems of diarrhea and the like of the test dog, while the commercial C3 Weishi control nutrition cream has no obvious influence on the feces on the whole, but has slight diarrhea phenomenon.

TABLE 2-8 Effect of puppy Nutrition pastes on stool status of test dogs

6. Mental state

As shown in tables 2-9, the mental states of three groups of the test puppies were very active throughout the test period. When the testers pass, all the three groups of test dogs bark, tail shake, rail climbing and forepaw extending, the mental state score reaches 7 points, and all the dogs have no phenomenon of listlessness or lying down. The results show that neither the A3 puppy nutrition cream nor the B3 puppy nutrition cream adversely affects the mental state of the test dogs.

TABLE 2-9 Effect of puppy Nutrition pastes on mental status of test dogs

Combining the results, the A3 puppy nutrition paste and the B3 puppy nutrition paste as the puppy nutrition supplement have remarkable food calling and growth promoting effects on test dogs, wherein the A3 puppy nutrition paste has better effects than the B3 puppy nutrition paste, and the A3 and B3 nutrition pastes have positive effects on the fur state of the test puppies and have no adverse effect on feed intake, skin state, fecal state, mental state and the like.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit of the corresponding technical solutions of the embodiments of the present invention.