阅读说明：本技术 一种分子诊断和化学发光检测一体机 (Molecular diagnosis and chemiluminescence detection all-in-one machine ) 是由 唐明忠 于 2021-07-30 设计创作，主要内容包括：本发明提供了一种分子诊断和化学发光检测一体机,上述分子诊断和化学发光检测一体机包括分子诊断检测系统、化学发光检测系统、多头核酸提取系统、检测板以及多头加液系统,上述化学发光检测系统与上述检测板在工作状态下接触连接,上述分子诊断检测系统与上述检测板相互对应,上述多头核酸提取系统和上述多头加液系统在上述检测板的上部；上述检测板上设有燕尾槽,上述燕尾槽位于上述检测板的尾部,上述检测板还包括盖板,上述盖板与上述燕尾槽配合连接,上述盖板与上述燕尾槽配合连接为两档配合连接,上述两档配合连接包括开盖连接和关盖连接,上述盖板在外力作用下由上述开盖连接变为上述关盖连接。同时实现了化学发光和分子检测的功能。(The invention provides a molecular diagnosis and chemiluminescence detection integrated machine, which comprises a molecular diagnosis detection system, a chemiluminescence detection system, a multi-head nucleic acid extraction system, a detection plate and a multi-head liquid adding system, wherein the chemiluminescence detection system is in contact connection with the detection plate in a working state, the molecular diagnosis detection system corresponds to the detection plate, and the multi-head nucleic acid extraction system and the multi-head liquid adding system are arranged on the upper part of the detection plate; the detection plate is provided with a dovetail groove, the dovetail groove is positioned at the tail part of the detection plate, the detection plate further comprises a cover plate, the cover plate is connected with the dovetail groove in a matched mode in two grades, the two grades are connected in the matched mode and comprise an uncovering connection and a closing connection, and the cover plate is changed from the uncovering connection to the closing connection under the action of external force. Simultaneously realizes the functions of chemiluminescence and molecular detection.)

1. The molecular diagnosis and chemiluminescence detection integrated machine is characterized by comprising a molecular diagnosis detection system (1), a chemiluminescence detection system (2), a multi-head nucleic acid extraction system (3), a detection plate (4) and a multi-head liquid adding system (5), wherein the chemiluminescence detection system (2) is in contact connection with the detection plate (4) in a working state, the molecular diagnosis detection system (1) and the detection plate (4) correspond to each other, and the multi-head nucleic acid extraction system (3) and the multi-head liquid adding system (5) are arranged on the upper part of the detection plate (4);

the detection plate is characterized in that a dovetail groove (42) is formed in the detection plate (4), the dovetail groove (42) is located at the tail of the detection plate (4), the detection plate (4) further comprises a cover plate (41), the cover plate (41) is connected with the dovetail groove (42) in a matched mode in a two-gear matched mode, the two-gear matched mode comprises cover opening connection and cover closing connection, and the cover plate (41) is changed from the cover opening connection into the cover closing connection under the action of external force.

2. The integrated molecular diagnostic and chemiluminescent detection system according to claim 1 wherein the detection plate (4) moves in the direction of the horizontal axis relative to the integrated molecular diagnostic and chemiluminescent detection machine, the molecular diagnostic detection system (1) and the chemiluminescent detection system (2) move in the direction of the vertical axis relative to the integrated molecular diagnostic and chemiluminescent detection machine, and the multi-head nucleic acid extraction system (3) and the multi-head liquid feeding system (5) move in the direction of the vertical axis relative to the integrated molecular diagnostic and chemiluminescent detection machine.

3. The integrated machine for molecular diagnosis and chemiluminescence detection according to claim 2, wherein a reaction cup (43) is placed in the detection plate (4), the molecular diagnosis detection system (1) is arranged at the side or upper part of the reaction cup (43), and the chemiluminescence detection system (2) is arranged at the upper part or side part of the reaction cup (43);

the molecular diagnosis detection system (1) and the detection plate (4) correspond to each other, namely, the molecular diagnosis detection system (1) moves from the side part or the upper part of the reaction cup (43) to the corresponding position of the reaction cup (43) of the detection plate (4) to realize the photographing function required by the molecular diagnosis detection system (1);

the chemical luminescence detection system (2) is in contact connection with the detection plate (4) in a working state, that is, the chemical luminescence detection system (2) moves from the upper part or the side part of the reaction cup (43) to the upper part of the reaction cup (43) and is in contact connection with the reaction cup (43) to realize the light-shielding function required by the chemical luminescence detection system (2).

4. The integrated molecular diagnosis and chemiluminescence detection machine according to claim 3, wherein the dovetail groove (42) is fixedly connected with the detection plate (4) to form an integral structure, the cover plate (41) is provided with positioning pins, the dovetail groove (42) is provided with positioning pin holes, the positioning pins are matched and connected with the positioning pin holes, the detection plate (4) moves relative to the integrated molecular diagnosis and chemiluminescence detection machine in a transverse axis direction, so that the cover plate (41) is changed from the open cover connection to the closed cover connection under the action of external force, the open cover connection enables the reaction cup (43) to be in an unclosed state, and the closed cover connection enables the reaction cup (43) to be in a closed state.

5. The integrated molecular diagnosis and chemiluminescence detection machine according to claim 4, wherein the integrated molecular diagnosis and chemiluminescence detection machine is further provided with a cross beam (11), the cross beam (11) is located between the molecular diagnosis detection system (1) and the detection plate (4), and the external force is that when the detection plate (4) moves in a transverse axis direction relative to the integrated molecular diagnosis and chemiluminescence detection machine, the cross beam (11) pushes the cover plate (41) to change the connection relationship between the cover plate (41) and the dovetail groove (42) from the open cover connection to the closed cover connection.

6. The integrated molecular diagnosis and chemiluminescence detection machine according to claim 4, wherein the cover plate (41) is provided with two positioning pins, the dovetail groove (42) is provided with four positioning pin holes, the two positioning pins are a first positioning pin (411) and a second positioning pin (412), and the four positioning pin holes are a first positioning pin hole (421), a second positioning pin hole (422), a third positioning pin hole (423) and a fourth positioning pin hole (424);

the first positioning pin (411) and the second positioning pin (412) are respectively inserted into the first positioning pin hole (421) and the second positioning pin hole (422) to be in matched connection for cover opening connection, and the first positioning pin (411) and the second positioning pin (412) are respectively inserted into the third positioning pin hole (423) and the fourth positioning pin hole (424) to be in matched connection for cover closing connection.

7. The integrated machine for molecular diagnosis and chemiluminescence detection according to claim 6, wherein a dovetail push plate (413) is provided at one end of the cover plate (41), the cover plate (41) further comprises a cover plate plane (414), the dovetail push plate (413) and the cover plate plane (414) form a first included angle (415), and the first included angle (415) is greater than 0 degree and less than 180 degrees;

the tail of the dovetail groove (42) is provided with a dovetail baffle (425), the dovetail groove (42) further comprises a dovetail groove plane (426), the dovetail baffle (425) and the dovetail groove plane (426) form a second included angle (427), and the second included angle (427) is larger than 0 degree and smaller than 180 degrees.

8. The integrated machine of claim 7, wherein the degree of the first included angle (415) is equal to the degree of the second included angle (427); and/or

The degree of said first included angle (415) is equal to 90 degrees and/or the degree of said second included angle (427) is equal to 90 degrees.

9. The integrated molecular diagnostic and chemiluminescent detection machine according to claim 7 wherein the first locating pin (411) and the second locating pin (412) are located at the front end of the cover plate plane (414), the front end of the cover plate plane (414) being the other end of the cover plate (41);

the first positioning pin hole (421) and the second positioning pin hole (422) are located at the rear end of the dovetail groove plane (426), the third positioning pin hole (423) and the fourth positioning pin hole (424) are located at the front end of the dovetail groove plane (426), and the distance between the rear end of the dovetail groove plane (426) and the dovetail baffle plate (425) is smaller than the distance between the front end of the dovetail groove plane (426) and the dovetail baffle plate (425).

10. The molecular detection plate according to claim 3, wherein the detection plate (4) further comprises a reaction chamber (44), a magnetic bead position (45), a reagent position (46), a magnetic sleeve position (47), a sample tube position (48) and a liquid suction head (49), the reaction cup (43), the reaction chamber (44), the magnetic bead position (45), the reagent position (46), the magnetic sleeve position (47), the sample tube position (48) and the liquid suction head (49) are sequentially positioned in the detection plate (4), a detection reagent is pre-sealed in the magnetic bead position (45) and the reagent position (46), and the reaction cup (43), the reaction chamber (44), the magnetic sleeve position (47), the sample tube position (48) and the liquid suction head (49) are in a non-sealed state.

11. Molecular assay plate according to claim 10, wherein the number of reagent sites (46) is equal to one or more than one.

12. The integrated molecular diagnosis and chemiluminescence detection machine according to claim 3, further comprising a molecular diagnosis temperature control system (6), wherein the molecular diagnosis temperature control system (6) wraps the outer wall of the reaction cup (43) to enable the reaction cup (43) to be heated uniformly.

13. The integrated machine for molecular diagnosis and chemiluminescence detection according to claim 12, wherein the molecular diagnosis temperature control system (6) is wrapped around the outer wall of the reaction cup (43) in a "U" shape.

14. The integrated molecular diagnostic and chemiluminescent detection machine of claim 12 wherein the molecular diagnostic temperature control system (6) is operable with the chemiluminescent detection system (2); or the molecular diagnosis and chemiluminescence detection integrated machine further comprises a chemiluminescence temperature control system (7), and the chemiluminescence temperature control system (7) is arranged at the bottom of the detection plate (4).

15. The integrated machine of molecular diagnostics and chemiluminescent detection according to claim 10, the multi-head nucleic acid extraction system (3) and the multi-head liquid adding system (5) move in the vertical axis direction relative to the molecular diagnosis and chemiluminescence detection all-in-one machine, the detection plate (4) moves in the direction of a transverse axis relative to the molecular diagnosis and chemiluminescence detection integrated machine, so that the multi-head nucleic acid extraction system (3) and the multi-head liquid adding system (5) respectively penetrate, contact or do not contact the reaction cup (43), the reaction bin (44), the magnetic bead position (45), the reagent position (46), the magnetic sleeve position (47), the sample tube position (48) and the liquid suction head (49) of the detection plate (4) according to the detection requirements of the molecular diagnosis detection system (1) and the chemiluminescence detection system (2).

Technical Field

The invention relates to the field of in-vitro diagnosis molecular detection instruments, in particular to an integrated machine for molecular diagnosis and chemiluminescence detection.

Background

The present in vitro diagnosis molecular detector is mainly used for molecular diagnosis, which is a technology for diagnosing by detecting the structure or expression level change of genetic material in a patient body by applying a molecular biology method, the molecular diagnosis is a main method for predicting diagnosis, and can be used for diagnosing individual genetic diseases and prenatal diagnosis, and the molecular diagnosis mainly refers to the detection of various structural proteins, enzymes, antigen antibodies and immune active molecular genes related to the diseases.

Chemiluminescence is a light radiation phenomenon accompanying substances in a chemical reaction process, can be divided into direct luminescence and indirect luminescence, the direct luminescence is the simplest chemiluminescence reaction, and comprises two key steps: i.e. excitation and radiation, e.g. A, B, chemically react to form C species, the energy released by the reaction being absorbed by the C species molecules and transferred to excited state C^＊At excited C^＊Generating optical radiation during the return to the ground state, where C^＊Is a luminous body, and in the process, the substance C directly participates in the reaction, so the direct chemiluminescence is called; indirect luminescence, also known as energy transfer chemiluminescence, is mainly composed of three steps: firstly, reactants A and B react to generate an excited intermediate C^＊(energy donor) when C^＊When decomposed, energy is released and transferred to F (energy acceptor), so that F is excited and transited to excited state F^＊And finally, when F^＊When the light returns to the ground state, light emission occurs.

In the prior art, a molecular diagnosis platform and a chemiluminescence platform are two independent platforms, and because the requirements of the environment and the consumable materials required by the two platforms are different, the molecular detection and the chemiluminescence detection can be completed by two or more instruments in the prior art, but for some small and medium hospitals, resources and environments sometimes do not allow the small and medium hospitals to be provided with the two independent platforms, and one instrument which can be used for both chemiluminescence and molecular diagnosis can be the first choice of the two independent platforms, so that an all-in-one machine for molecular diagnosis and chemiluminescence detection is to be provided.

Disclosure of Invention

The invention provides a molecular diagnosis and chemiluminescence detection integrated machine, which at least solves the technical problem that in the prior art, a molecular diagnosis platform and a chemiluminescence platform are two independent platforms, and one machine cannot simultaneously meet the requirements of molecular diagnosis and chemiluminescence detection under the condition of limited resources of medium and small hospitals.

The invention provides a molecular diagnosis and chemiluminescence detection integrated machine, which comprises a molecular diagnosis detection system, a chemiluminescence detection system, a multi-head nucleic acid extraction system, a detection plate and a multi-head liquid adding system, wherein the chemiluminescence detection system is in contact connection with the detection plate in a working state, the molecular diagnosis detection system corresponds to the detection plate, and the multi-head nucleic acid extraction system and the multi-head liquid adding system are arranged on the upper part of the detection plate;

the detection plate is provided with a dovetail groove, the dovetail groove is positioned at the tail part of the detection plate, the detection plate further comprises a cover plate, the cover plate is connected with the dovetail groove in a matched mode in two grades, the two grades are connected in the matched mode and comprise an uncovering connection and a closing connection, and the cover plate is changed from the uncovering connection to the closing connection under the action of external force.

Optionally, the detection plate moves in a horizontal axis direction relative to the integrated molecular diagnosis and chemiluminescence detection machine, the molecular diagnosis detection system 1 and the chemiluminescence detection system move in a vertical axis direction relative to the integrated molecular diagnosis and chemiluminescence detection machine, and the multi-head nucleic acid extraction system and the multi-head liquid adding system move in a vertical axis direction relative to the integrated molecular diagnosis and chemiluminescence detection machine.

Optionally, a reaction cup is placed in the detection plate, the molecular diagnosis detection system is arranged on the side part or the upper part of the reaction cup, and the chemiluminescence detection system is arranged on the upper part or the side part of the reaction cup;

the molecular diagnosis detection system and the detection plate correspond to each other, namely the molecular diagnosis detection system moves from the side part or the upper part of the reaction cup to the corresponding position of the reaction cup of the detection plate so as to realize the photographing function required by the molecular diagnosis detection system;

the contact connection of the chemiluminescence detection system and the detection plate in a working state means that the chemiluminescence detection system moves from the upper part or the side part of the reaction cup to the upper part of the reaction cup and is in contact connection with the reaction cup to realize the light-shielding function required by the chemiluminescence detection system.

Optionally, the dovetail groove is fixedly connected with the detection plate as an integral structure, the cover plate is provided with a positioning pin, the dovetail groove is provided with a positioning pin hole, the positioning pin is in fit connection with the positioning pin hole, the detection plate moves in a transverse axis direction relative to the molecular diagnosis and chemiluminescence detection integrated machine, so that the cover plate is changed from the open-cover connection to the close-cover connection under the action of external force, the open-cover connection enables the reaction cup to be in a non-closed state, and the close-cover connection enables the reaction cup to be in a closed state.

Optionally, the integrated molecular diagnosis and chemiluminescence detection machine further comprises a cross beam located between the molecular diagnosis detection system and the detection plate, and the external force is that when the detection plate moves in a transverse axis direction relative to the integrated molecular diagnosis and chemiluminescence detection machine, the cross beam pushes the cover plate to change the connection relationship between the cover plate and the dovetail groove from the open cover connection to the closed cover connection.

Optionally, two positioning pins are arranged on the cover plate, four positioning pin holes are arranged on the dovetail groove, the two positioning pins are respectively a first positioning pin and a second positioning pin, and the four positioning pin holes are respectively a first positioning pin hole, a second positioning pin hole, a third positioning pin hole and a fourth positioning pin hole;

the first positioning pin and the second positioning pin are respectively inserted into the first positioning pin hole and the second positioning pin hole to be in matched connection with each other for opening the cover, and the first positioning pin and the second positioning pin are respectively inserted into the third positioning pin hole and the fourth positioning pin hole to be in matched connection with each other for closing the cover.

Optionally, a dovetail push plate is arranged at one end of the cover plate, the cover plate further comprises a cover plate plane, a first included angle is formed between the dovetail push plate and the cover plate plane, and the first included angle is greater than 0 degree and smaller than 180 degrees;

the dovetail groove further comprises a dovetail groove plane, a second included angle is formed between the dovetail baffle and the dovetail groove plane, and the second included angle is larger than 0 degree and smaller than 180 degrees.

Optionally, the degree of the first included angle is equal to the degree of the second included angle; and/or

The degree of the first included angle is equal to 90 degrees, and/or the degree of the second included angle is equal to 90 degrees.

Optionally, the first positioning pin and the second positioning pin are located at a front end of the cover plate plane, and the front end of the cover plate plane is the other end of the cover plate;

the first positioning pin hole and the second positioning pin hole are located at the rear end of the dovetail groove plane, the third positioning pin hole and the fourth positioning pin hole are located at the front end of the dovetail groove plane, and the distance between the rear end of the dovetail groove plane and the dovetail baffle is smaller than the distance between the front end of the dovetail groove plane and the dovetail baffle.

Optionally, the detection plate further includes a reaction bin, a magnetic bead position, a reagent position, a magnetic sleeve position, a sample tube position, and a liquid suction head, the reaction cup, the reaction bin, the magnetic bead position, the reagent position, the magnetic sleeve position, the sample tube position, and the liquid suction head are sequentially located in the detection plate, a detection reagent is sealed in the magnetic bead position and the reagent position in advance, and the reaction cup, the reaction bin, the magnetic sleeve position, the sample tube position, and the liquid suction head are in a non-sealed state.

Optionally, the number of the reagent sites is equal to or greater than one.

Optionally, the integrated molecular diagnosis and chemiluminescence detection machine further comprises a molecular diagnosis temperature control system, and the molecular diagnosis temperature control system wraps the outer wall of the reaction cup to enable the reaction cup to be heated uniformly.

Optionally, the molecular diagnosis temperature control system is wrapped on the outer wall of the reaction cup in a U shape.

Optionally, the molecular diagnosis temperature control system may be used for the chemiluminescence detection system; or the molecular diagnosis and chemiluminescence detection integrated machine further comprises a chemiluminescence temperature control system, and the chemiluminescence temperature control system is arranged at the bottom of the detection plate.

Optionally, the multi-head nucleic acid extraction system and the multi-head liquid adding system move in a vertical axis direction relative to the integrated molecular diagnosis and chemiluminescence detection machine, and the detection plate moves in a horizontal axis direction relative to the integrated molecular diagnosis and chemiluminescence detection machine, so that the multi-head nucleic acid extraction system and the multi-head liquid adding system respectively penetrate into, contact with or do not contact with the reaction cup, the reaction chamber, the magnetic bead position, the reagent position, the magnetic sleeve position, the sample tube position and the liquid sucking head of the detection plate according to the detection requirements of the molecular diagnosis detection system and the chemiluminescence detection system.

The detection system and the temperature control system of the molecular diagnosis platform and the chemiluminescence platform in the prior art are different from each other, but the technical scheme of the invention solves the technical problem of simultaneously meeting the detection system and the temperature control system of the molecular diagnosis platform and the chemiluminescence platform, namely, the detection system and the temperature control system of the molecular diagnosis and chemiluminescence detection integrated machine are ingeniously arranged above and at the side of the reaction cup position of the detection plate, and the temperature control system can meet the temperature control requirements of the reaction cup for molecular detection and chemiluminescence and is uniformly arranged below the reaction cup.

On the other hand, the technical problem is solved by the novel molecular detection plate included in the molecular diagnosis and chemiluminescence detection integrated machine, the problem of cover closing is solved by the detection plate, so that a precondition is provided for realizing automation, and a space is provided for a molecular diagnosis detection system and a chemiluminescence detection system by skillfully designing a dovetail groove.

The detection plate solves the technical problems that when an oil seal is heated at high temperature, an amplification product escapes along with steam, a film sealing belt needs to be manually added and a waste material belt needs to be removed due to complex film sealing action, and a cover has a certain air leakage probability risk due to complex sealing action.

The use of chemiluminescence requires magnetic bead washing and transfer, and the need for sample and liquid addition, which is the same as the function of the nucleic acid extraction and reagent addition system for molecular diagnostics and can therefore be designed as the same part. The different places are consumable materials of molecular diagnosis, temperature control and detection systems are different, but all the consumable materials can be integrated on the same machine.

The machine realizes the functions of chemiluminescence and molecular detection, and can freely switch between chemiluminescence and molecular diagnosis.

Can realize on the consumptive material that nucleic acid extraction and corresponding chemiluminescence's magnetic bead wash, the application of sample, add reagent, and temperature control system, detecting system, the overall arrangement that all corresponds is on the consumptive material of two methodologies, and perfect integrates the function of two platforms to same platform on.

Drawings

The above and other objects, features and advantages of exemplary embodiments of the present invention will become readily apparent from the following detailed description read in conjunction with the accompanying drawings. Several embodiments of the invention are illustrated by way of example, and not by way of limitation, in the figures of the accompanying drawings and in which:

FIG. 1 is a schematic diagram of an alternative integrated molecular diagnostic and chemiluminescent detection system in accordance with an embodiment of the present invention;

FIG. 2 is a schematic diagram of the structural relationship of the main components in an alternative integrated machine for molecular diagnosis and chemiluminescence detection and the principle of molecular detection according to an embodiment of the invention;

FIG. 3 is a schematic view of the operation principle of the alternative molecular diagnostic test system and the test board according to the embodiment of the present invention;

fig. 4 is a schematic structural view of an alternative uncapped state of the detection board according to the embodiment of the present invention;

FIG. 5 is a schematic view of an alternative detection panel of an embodiment of the present invention in a closed position;

FIG. 6 is a schematic view of an alternative integrated molecular diagnostic and chemiluminescent detection machine having an extended beam according to embodiments of the present invention;

FIG. 7 is a schematic view of an alternative molecular diagnostic and chemiluminescent detection engine with an extended beam and showing vertical axis gears in accordance with embodiments of the present invention;

FIG. 8 is a schematic view of an alternative cover plate for the detector plate according to an embodiment of the present invention;

FIG. 9 is a schematic structural diagram of a dovetail groove of an alternative detection plate according to an embodiment of the present invention;

FIG. 10 is a schematic view of an alternative drop-out frame rail of an embodiment of the present invention shown without contacting the cover plate;

FIG. 11 is a schematic view of an alternative reach frame rail contacting the cover plate in accordance with an embodiment of the present invention;

FIG. 12 is a schematic view of an alternative embodiment of the present invention showing an extended frame beam pushing the cover in an auto-close position;

fig. 13 is a schematic external view of an alternative integrated molecular diagnosis and chemiluminescence detection apparatus according to an embodiment of the invention.

The following detailed description is intended to further illustrate but not limit the invention, the following example being only one preferred embodiment of the invention.

Detailed Description

The principles and spirit of the present invention will be described with reference to a number of exemplary embodiments. It is understood that these embodiments are given solely for the purpose of enabling those skilled in the art to better understand and to practice the invention, and are not intended to limit the scope of the invention in any way. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.

As shown in fig. 1 and fig. 2, wherein fig. 1 is a schematic diagram of an optional integrated machine for molecular diagnosis and chemiluminescence detection provided by an embodiment of the present invention, fig. 2 is a schematic diagram of a structural relationship and a molecular detection principle of main components inside the optional integrated machine for molecular diagnosis and chemiluminescence detection provided by an embodiment of the present invention, the integrated machine for molecular diagnosis and chemiluminescence detection comprises a molecular diagnosis detection system 1, a chemiluminescence detection system 2, a multi-head nucleic acid extraction system 3, a detection plate 4 and a multi-head liquid adding system 5, the chemiluminescence detection system 2 is in contact connection with the detection plate 4 in a working state, that is, the chemiluminescence detection system 2 is aligned with and in contact with a chemiluminescent reagent hole site of the detection plate 4 so as to provide a light-shielding condition for chemiluminescence detection, as shown in fig. 3, the molecular diagnosis detection system 1 corresponds to the detection plate 4, that is, the above-mentioned molecular diagnosis detecting system 1 and the above-mentioned molecular diagnosis test agent well of the above-mentioned detecting plate 4 correspond to each other so as to be able to take the required molecular photograph for molecular diagnosis, the above-mentioned multi-head nucleic acid extracting system 3 and the above-mentioned multi-head liquid adding system 5 are on the upper portion of the above-mentioned detecting plate 4, as shown in FIG. 2;

as shown in fig. 4, the detection plate 4 is provided with a dovetail groove 42, the dovetail groove 42 is located at the tail of the detection plate 4, the detection plate 4 further includes a cover plate 41, the cover plate 41 is in fit connection with the dovetail groove 42 in two stages, the two stages of fit connection includes an open cover connection and a close cover connection, and the cover plate 41 is changed from the open cover connection to the close cover connection by an external force, as shown in fig. 5.

The embodiment of the invention provides a molecular diagnosis and chemiluminescence detection integrated machine, which comprises a molecular detection plate capable of automatically closing a cover, wherein a positioning pin is arranged on a cover plate, the cover plate is positioned at a cover opening position before a PCR experiment, the cover plate is pushed to a cover closing position before the PCR experiment is started, namely the cover plate 41 is inserted into a reaction cup position of a dovetail groove 42 after being stressed, and the cover plate 41 stops moving when being pressed to the position.

The consumable material detection plate 4 is easy to realize automation, the cover plate can be tightly closed only by force in one direction, namely the cover plate 41 can be tightly closed by the force of the cover plate 41 to the dovetail groove structure 42 shown in the attached drawing 4, the force in the direction can be shared by a position switching motor for sample adding and nucleic acid extraction, the space and the cost are saved, and the operation is simpler.

Therefore, the cover and the detection consumable are integrated, the cover is a part of the consumable detection plate 4, and can be automatically closed in the use process.

Further, the detection plate 4 is moved in the lateral axis direction with respect to the integrated molecular diagnosis and chemiluminescence detection apparatus, the molecular diagnosis detection system 1 and the chemiluminescence detection system 2 are moved in the longitudinal axis direction with respect to the integrated molecular diagnosis and chemiluminescence detection apparatus, and the multi-head nucleic acid extraction system 3 and the multi-head liquid addition system 5 are moved in the vertical axis direction with respect to the integrated molecular diagnosis and chemiluminescence detection apparatus.

Further, as shown in FIG. 4, a cuvette 43 is placed in the detection plate 4, the molecular diagnosis detection system 1 is provided on the side or upper portion of the cuvette 43, and the chemiluminescence detection system 2 is provided on the upper or side portion of the cuvette 43;

the molecular diagnosis detecting system 1 and the detecting plate 4 correspond to each other, in which the molecular diagnosis detecting system 1 moves from a side portion or an upper portion of the cuvette 43 to a position corresponding to the cuvette 43 of the detecting plate 4 to realize a photographing function required for the molecular diagnosis detecting system 1; because the gear can move the above molecular diagnostic test system 1 in the vertical axis direction.

The contact connection of the chemiluminescence detection system 2 and the detection plate 4 in an operating state means that the chemiluminescence detection system 2 moves from the upper part or the side part of the reaction cup 43 to the upper part of the reaction cup 43 and is in contact connection with the reaction cup 43 to realize a light-shielding function required by the chemiluminescence detection system 2.

The structure of the cover plate 41 and the dovetail groove 42 ensures that when the cover plate 41 is pushed in place, the reaction cup 43 of the detection plate 4 is in interference fit with the cover plate 41 and is tightly pressed together, the positioning pin of the cover plate 41 is clamped into the hole of the consumable, the structure of the dovetail groove 42 ensures compaction, the positioning pin ensures locking, and the sealing of the cover and the reaction tube is ensured.

When the selected mode is molecular diagnosis, a molecular diagnosis consumable is inserted, a sample tube is placed into the sample position of the consumable detection plate 4, detection is started, the instrument performs nucleic acid extraction according to the nucleic acid extraction flow, then the multi-head liquid adding system adds a reagent system into the reaction cup 43, a cover closing program is started, PCR amplification and fluorescence detection are performed, and after the test is finished, a detection report is given by an interface.

When the selected mode is chemiluminescence, inserting a chemiluminescence consumable, placing a sample tube into a sample position of the consumable detection plate 4, starting detection, carrying out sample reagent reaction according to a chemiluminescence reaction process by using an instrument, incubating, cleaning magnetic beads by using a nucleic acid extraction system, adding a reagent system into the reaction tube by using a multi-head liquid adding system, detecting the quantity of photons in the reaction tube by using the detection system, and after the test is finished, giving a detection report by using an interface.

Further, the dovetail groove 42 is fixedly connected to the detection plate 4 as an integral structure, the cover plate 41 is provided with a positioning pin, the dovetail groove 42 is provided with a positioning pin hole, the positioning pin is engaged with the positioning pin hole, the detection plate 4 moves in a horizontal axis direction relative to the integrated molecular diagnosis and chemiluminescence detection machine, so that the cover plate 41 is changed from the open-cover connection to the close-cover connection by an external force, the open-cover connection enables the reaction cup 43 to be in a non-closed state, and the close-cover connection enables the reaction cup 43 to be in a closed state.

Further, as shown in fig. 1, the integrated molecular diagnosis and chemiluminescence detection apparatus is further provided with a cross beam 11, the cross beam 11 is located between the molecular diagnosis detection system 1 and the detection plate 4, and the external force is a force that the cross beam 11 pushes the cover plate 41 to change the connection relationship between the cover plate 41 and the dovetail groove 42 from the open cover connection to the closed cover connection when the detection plate 4 moves in the horizontal axis direction with respect to the integrated molecular diagnosis and chemiluminescence detection apparatus.

Alternatively, as shown in fig. 6, fig. 6 is a molecular diagnosis and chemiluminescence detection integrated machine of a preferred variation of the present embodiment, and specifically, is preferably embodied by a modification of the cross beam 11, and the cross beam 11 is preferably modified to be a protruding frame cross beam 12 having a protruding structure as shown in fig. 6, that is, a distance from the detection plate 4 is shorter than the original cross beam 11, and when the cross beam 11 pushes the cover plate 41 of the detection plate 4, the cross beam 11 needs to be closer to the detection plate 4 together with the molecular diagnosis detection system 1, that is, the displacement of the cross axis is larger, as seen from the fact that the distance between the cross beam 11 and the detection plate 4 is larger than the distance between the protruding frame cross beam 12 and the detection plate 4.

Further, as shown in fig. 7, the molecular diagnosis and chemiluminescence detection all-in-one machine further comprises a vertical shaft gear 21, the vertical axis gear 21 is positioned on the side of the chemiluminescence detection system 2, and is engaged with a member to which the chemiluminescence detection system 2 is fixedly attached, that is, the vertical shaft gear 21 rotates to drive the chemiluminescence detection system 2 to move up and down, and the chemiluminescence detection system 2 is aligned and contacted with the hole to be detected of the detection plate 4 by matching with the mechanical slide ways on the horizontal shaft and the vertical shaft to meet the requirement of chemiluminescence detection, at the same time, the extension-type frame beam 12 changes the cover plate 41 from the open-cover connection to the close-cover connection in accordance with the movement of the detection plate 4 on the lateral axis, the open-lid connection brings the cuvette 43 into an open state, and the close-lid connection brings the cuvette 43 into a closed state.

Further, as shown in fig. 8 and 9, the cover plate 41 is provided with two positioning pins, the dovetail groove 42 is provided with four positioning pin holes, the two positioning pins are a first positioning pin 411 and a second positioning pin 412, respectively, and the four positioning pin holes are a first positioning pin hole 421, a second positioning pin hole 422, a third positioning pin hole 423, and a fourth positioning pin hole 424, respectively;

the fitting connection in which the first positioning pin 411 and the second positioning pin 412 are inserted into the first positioning pin hole 421 and the second positioning pin hole 422 is an open-lid connection, and the fitting connection in which the first positioning pin 411 and the second positioning pin 412 are inserted into the third positioning pin hole 423 and the fourth positioning pin hole 424 is a closed-lid connection.

Further, as shown in fig. 8 and 9, a dovetail push plate 413 is disposed at one end of the cover plate 41, the cover plate 41 further includes a cover plate plane 414, the dovetail push plate 413 and the cover plate plane 414 form a first included angle 415, and the first included angle 415 is greater than 0 degree and less than 180 degrees;

a dovetail flap 425 is provided at a tail portion of the dovetail groove 42, the dovetail groove 42 further includes a dovetail groove plane 426, the dovetail flap 425 and the dovetail groove plane 426 have a second included angle 427, and the second included angle 427 is greater than 0 degree and less than 180 degrees.

Further, the degree of the first included angle 415 is equal to the degree of the second included angle 427; and/or

The degree of the first included angle 415 is equal to 90 degrees, and/or the degree of the second included angle 427 is equal to 90 degrees.

Further, the first positioning pin 411 and the second positioning pin 412 are located at the front end of the cover plate plane 414, and the front end of the cover plate plane 414 is the other end of the cover plate 41;

the first positioning pin holes 421 and the second positioning pin holes 422 are located at the rear end of the dovetail groove plane 426, the third positioning pin holes 423 and the fourth positioning pin holes 424 are located at the front end of the dovetail groove plane 426, and the distance between the rear end of the dovetail groove plane 426 and the dovetail baffle 425 is smaller than the distance between the front end of the dovetail groove plane 426 and the dovetail baffle 425.

Further, as shown in fig. 4, the detection plate 4 further includes a reaction chamber 44, a magnetic bead position 45, a reagent position 46, a magnetic sleeve position 47, a sample tube position 48, and a pipette tip 49, the reaction cup 43, the reaction chamber 44, the magnetic bead position 45, the reagent position 46, the magnetic sleeve position 47, the sample tube position 48, and the pipette tip 49 are sequentially located in the detection plate 4, detection reagents are sealed in the magnetic bead position 45 and the reagent position 46 in advance, and the reaction cup 43, the reaction chamber 44, the magnetic sleeve position 47, the sample tube position 48, and the pipette tip 49 are in a non-sealed state.

Further, the number of the reagent sites 46 is equal to or greater than one.

Further, as shown in fig. 2 and 4, the integrated molecular diagnosis and chemiluminescence detection machine further includes a molecular diagnosis temperature control system 6, and the molecular diagnosis temperature control system 6 wraps the outer wall of the reaction cup 43 to make the reaction cup 43 heated uniformly.

Further, the molecular diagnosis temperature control system 6 is in a "U" shape and wraps the outer wall of the reaction cup 43.

Further, as shown in fig. 2, the molecular diagnostic temperature control system 6 can be used in the chemiluminescence detection system 2; or the molecular diagnosis and chemiluminescence detection integrated machine further comprises a chemiluminescence temperature control system 7, and the chemiluminescence temperature control system 7 is arranged at the bottom of the detection plate 4.

The main difference between the consumable of molecular diagnosis and the consumable of chemiluminescence is the difference between the consumable cover plate of molecular diagnosis, namely the cover plate 41 of the detection plate 4, the structure of the consumable is basically consistent, while the difference of the whole machine is the detection system, the molecular diagnosis detection system 1 is arranged at the side surface of the reaction tube of the detection plate 4, the chemiluminescence detection system 2 is arranged above the detection plate 4, the other difference is the temperature control system, the molecular temperature control system is arranged at the side surface of the reaction tube, the chemiluminescence temperature control system is arranged below the reaction tube, and the common multi-head liquid adding system and the nucleic acid extraction system correspond to the chemiluminescence magnetic bead cleaning system.

Further, the multi-head nucleic acid extracting system 3 and the multi-head liquid adding system 5 are moved in the vertical axis direction with respect to the integrated molecular diagnosis and chemiluminescence detection system, and the detection plate 4 is moved in the horizontal axis direction with respect to the integrated molecular diagnosis and chemiluminescence detection system, so that the multi-head nucleic acid extracting system 3 and the multi-head liquid adding system 5 are inserted into, brought into contact with, or brought into contact with the cuvette 43, the reaction chamber 44, the bead position 45, the reagent position 46, the magnetic sleeve position 47, the sample tube position 48, and the liquid sucking head 49 of the detection plate 4, respectively, in accordance with the detection requirements of the molecular diagnosis detection system 1 and the chemiluminescence detection system 2.

The distance between the multi-head nucleic acid extraction system 3 and the detection plate 4 is greater than 5 mm, preferably 10-15 mm.

Fig. 10 is a view showing a state where the cover plate 41 on the detection plate 4 is not yet in contact with the cover plate 41, as shown in fig. 10, and when the cover plate 41 is further close to the projected frame cross member 12 on the horizontal axis, the cover plate 41 and the projected frame cross member 12 are in contact with each other as shown in fig. 11, and when the detection plate 4 is further moved on the horizontal axis, the cover plate 41 on the detection plate 4 is automatically closed by the urging force of the projected frame cross member 12 as shown in fig. 12, thereby providing a basis for the next molecular diagnosis and chemiluminescence detection.

As shown in FIG. 13, the molecular diagnosis and chemiluminescence detection integrated machine is small, compact, simple in structure, free of a liquid path system and reliable.

As described in this embodiment, the internal relationship of the molecular diagnosis and chemiluminescence integrated machine is as follows: the multi-head liquid adding system and the multi-head nucleic acid extracting system are arranged on the upper portion of a consumable, the chemiluminescence detecting system is arranged on the upper portion of a reaction cup, the molecular diagnosis detecting system is arranged on the side portion of the reaction cup, the chemiluminescence temperature control system is arranged at the bottom of the consumable, and the molecular diagnosis temperature control system is arranged on the left side and the right side of the reaction cup. The detection system for molecular diagnosis and chemiluminescence can also be placed at the upper, lower, left or right parts of the reaction cup as long as enough space is reserved.

The optional molecular diagnosis and chemiluminescence detection integrated machine provided by the embodiment of the invention has the following beneficial effects:

1. one machine realizes the functions of chemiluminescence and molecular detection;

2. nucleic acid extraction, cleaning of corresponding chemiluminescent magnetic beads, sample adding and reagent adding can be realized on the consumable detection plate;

3. the temperature control system and the detection system are correspondingly arranged on the consumables of the two methodologies.

The chemiluminescence is used for cleaning and transferring magnetic beads, a sample and liquid are added at the same time, the functions of the nucleic acid extraction and reagent adding system of the molecular diagnosis are the same, and therefore the same part can be designed, the different parts are consumables of the molecular diagnosis, the temperature control and the detection system are different, and the parts can be integrated on the same machine.

The consumable materials are all designed in a strip type disposable manner, and a placing reagent, a sample adding head, a detection tube and a sample tube are integrated in the consumable materials; the chemiluminescent temperature control system is arranged at the front half part of the consumable, the detection system is arranged at the top of the reaction cup, the molecular detection temperature control system is arranged at two sides of the reaction cup, and the detection system is arranged at the side surface of the cup; the negative pressure system of molecular diagnosis is on the top of the machine, wherein, a filtering membrane is used for filtering possible pollutants, an ultraviolet lamp is arranged on the top for removing the pollution in aerosol, a common control system, a man-machine interaction system, a power supply and a printer are arranged on the top.

The core of the invention is the improvement of the consumable detection plate, namely the design of automatic cover closing and tight sealing of the novel molecular detection plate, because the detection plate solves the problem of cover closing, thereby providing a precondition for realizing automation, and the ingenious design of the dovetail groove provides a space for a molecular diagnosis detection system and a chemiluminescence detection system.

Make two detecting system's position can both put down in the space, the integration of consumptive material detection board, the space has been saved in the improvement of consumptive material detection board, gives and puts into two detecting system simultaneously and become possible because the mechanism of closing the lid shares with the moving mechanism of consumptive material, and bull nucleic acid, inhale kind, close the lid also can share.