阅读说明：本技术 一种基于h2o2原位合成的uv/h2o2室内空气消毒技术 (Based on H2O2In situ synthesized UV/H2O2Indoor air sterilizing technology ) 是由 李楠 乔羽婕 于 2021-06-18 设计创作，主要内容包括：本发明公开了一种基于H-(2)O-(2)原位合成的紫外线(UV)/H-(2)O-(2)室内空气消毒技术。利用双电极系统中,使用Pt电极做阳极,炭黑/石墨空气呼吸电极做阴极通过电化学反应原位合成H-(2)O-(2),将雾化H-(2)O-(2)与UV消毒结合,高效进行室内消毒。空气中存在大量的细菌微生物,室外空气中常见的微生物有产芽胞杆菌、产色素细菌及真菌孢子等,室内空气中常见的病原菌有结核杆菌、溶血性球菌、白喉杆菌、百日咳杆菌等。但室内空气中的微生物的数量比室外更多,尤其是在公共场所、医院病房、门诊等人口密集的地方,容易受到带菌者和病人的污染。所以利用UV/H-(2)O-(2)结合消毒技术对室内空气进行灭菌,提高消毒效果和效率,UV处理对空气内细菌灭菌率为85％左右,在3min内仍有部分细菌存活,相比之下,UV/H-(2)O-(2)处理可以在3min内完全对空气内细菌进行灭菌(100％)。同时,原位生成H-(2)O-(2)也是一种绿色清洁生产工艺。(The invention discloses a method based on H 2 O 2 In situ synthesized Ultraviolet (UV)/H 2 O 2 Indoor air disinfection technology. H is synthesized in situ by electrochemical reaction by using Pt electrode as anode and carbon black/graphite air breathing electrode as cathode in a double-electrode system 2 O 2 Will atomize H 2 O 2 And the indoor disinfection is efficiently performed by combining with the UV disinfection. The air contains a large amount of bacterial microorganisms, and the common microorganisms in outdoor air include bacillus, pigment-producing bacteria and fungiSpores, etc., and the common pathogenic bacteria in indoor air include tubercle bacillus, hemolytic coccus, diphtheria bacillus, pertussis bacillus, etc. However, the amount of microorganisms in the indoor air is larger than that in the outdoor air, and particularly in places with dense population such as public places, hospital wards, clinics and the like, the indoor air is easily polluted by bacteria carriers and patients. So using UV/H 2 O 2 The sterilization technology is combined to sterilize indoor air, the sterilization effect and efficiency are improved, the sterilization rate of bacteria in the air by UV treatment is about 85%, and partial bacteria still survive within 3min 2 O 2 The treatment can completely sterilize the bacteria in the air within 3min (100%). Simultaneously, H is generated in situ 2 O 2 Is also a green clean production process.)

1. The invention utilizes carbon black/graphite air breathing cathode to produce H in situ₂O₂And a UV/H is prepared₂O₂Combined sterilizing devices using atomisation H₂O₂Sterilizing bacteria microorganism in air with ultraviolet lamps with different powers, comparing UV with UV/H₂O₂The bacteria removing effect under different disinfection processes.

2. The method of claim 1, comprising the steps of:

(1) the disinfection device is shown in figure 1. The detachable ultraviolet lamps are arranged around the device (15cm × 15cm × 15cm, 30cm × 30cm × 30cm, 45cm × 45cm × 45cm) in parallel, the ultraviolet devices adopt low-pressure ultraviolet lamps with different powers, and H is arranged at the top₂O₂The side lower part of the atomizing nozzle is provided with a movable opening for placing a nutrient agar plate for sampling.

(2)H₂O₂In situ generation of (a): h₂O₂The production of (a) was carried out in a two-electrode system in a single-chamber reactor (28mL, 3cm diameter and 4cm length), with a Pt electrode for the anode and a carbon black/graphite air breathing cathode for the cathode. H accumulated in 1H₂O₂(2044±126mg L^-1) Continuously atomizing and spraying into the disinfection device.

(3) Set control group experiment: setting single UV disinfection, UV/H₂O₂And (5) sterilizing. After the radiation of ultraviolet lamps with different powers for 0, 1, 3 and 5min, opening the container cover of the nutrient agar plate, placing the nutrient agar plate in devices with different sizes, exposing the nutrient agar plate in air for 5min, covering the container cover, turning the plate upside down, placing the nutrient agar plate in a constant-temperature incubator at 37 ℃ for culturing for 24h, and counting the number of colonies.

3. The manufacture of a disinfection device as claimed in claim 2, characterized in that in step (1): the ultraviolet lamp adopts violet with different powersOuter lamp (4-100W, 254nm) and atomization H₂O₂。

4. H according to claim 2₂O₂Characterized in that in step (4): carbon black/graphite air breathing electrode as cathode at 20mA cm^-2，50mM Na₂SO₄(pH 7) in situ formation of H in electrolyte solution₂O₂Accumulated H₂O₂For UV/H₂O₂And (6) sterilizing.

(I) technical field

The invention relates to the field of electrochemical synthesis, in particular to ultraviolet and H₂O₂In combination with techniques for indoor air disinfection.

(II) background of the invention

A large amount of bacterial microorganisms exist in the air, the microorganisms commonly found in outdoor air comprise bacillus, pigment-producing bacteria, fungal spores and the like, and the pathogenic bacteria commonly found in indoor air comprise tubercle bacillus, hemolytic coccus, diphtheria bacillus, bordetella pertussis and the like. However, the amount of microorganisms in the indoor air is larger than that in the outdoor air, and particularly in places with dense population such as public places, hospital wards, clinics and the like, the indoor air is easily polluted by bacteria carriers and patients.

Ultraviolet (UV)/H₂O₂The process is the most widely used Advanced Oxidation Process (AOP) for degradation and disinfection of organic compounds. With Fenton, UV/peroxodisulphate, UV/H₂O₂/NaHCO₃UV/H compared to other AOP₂O₂Hydroxyl free radical (. OH) plays a main role in the process to lead to the inactivation of microorganisms, other ions are not introduced in the process, and no harmful substance remains after the disinfection is finished. Atomized H₂O₂The fog drops have the advantages of small particle size, higher activation speed and the like, can permeate into all corners of air in a short time, effectively kill bacteria or viruses in the air, and meet the requirements of large area, short time effect and omnibearing disinfection. Simultaneous atomization of H₂O₂Then, ultraviolet ray to H₂O₂Has a higher transmittance of H₂O₂The photolysis is accelerated, more OH with higher activity is generated, and the sterilization effect is improved.

H₂O₂The in-situ synthesis of (A) is a green and efficient H generation₂O₂In this way, the green process avoids the traditional process of synthesizing H₂O₂Problems such as large energy input, complicated steps and much waste generated in the Anthraquinone Oxidation (AO) processEtc. while avoiding the problem at H₂O₂Potential hazards and additional costs associated with storage and handling during transportation.

Carbon-based materials are widely used as catalytic materials due to their advantages of large surface area, high electrical conductivity, corrosion resistance, high overall abundance, and low price. Currently, air breathing cathodes are a very potential cathode form in electrochemical systems. The air breathing cathode consists of a Catalytic Layer (CL) immersed in an electrolyte solution and a hydrophobic Gas Diffusion Layer (GDL) exposed to air, wherein oxygen in the air is diffused into the catalytic layer through the diffusion layer and generates H in situ by ORR reaction at the cathode₂O₂。

(III) purpose

The object of the invention is to produce H by using H which can be generated in situ₂O₂UV/H of₂O₂And a system for sterilizing indoor air. The carbon black/graphite air breathing cathode can produce H in situ in an electrochemical system in a green and efficient manner₂O₂By reacting H with₂O₂The atomized water is sprayed into the air, and UV radiation is combined, so that the indoor air disinfection efficiency and disinfection effect are further improved.

(IV) technical scheme

In order to solve the problems, the invention adopts the following technical scheme:

the invention utilizes carbon black/graphite air breathing cathode to produce H in situ₂O₂Will atomize H₂O₂And (3) combining with UV radiation, disinfecting air in a disinfecting device, collecting and culturing bacteria in the air before and after disinfection by adopting a flat plate exposure sedimentation method after disinfection, and observing the disinfection effect.

(1) A disinfection device: as shown in fig. 1. The device (15cm × 15cm × 15cm, 30cm × 30cm × 30cm, 45cm × 45cm × 45cm) comprises detachable ultraviolet lamps arranged in parallel at the periphery, low-pressure ultraviolet lamps (4-100W, 254nm) with different power and mounted on the top₂O₂The side lower part of the atomizing nozzle is provided with a movable opening for placing a nutrient agar plate for sampling.

(2) Preparing a nutrient agar culture medium: mixing peptone 10g and beefExtract 3g, NaCl 3g, NaH₂PO₄1g of agar, 20g of agar and 1000mL of distilled water are mixed and then heated to be dissolved, the pH value is adjusted to 7.8-8.0, impurities are filtered, the mixture is subpackaged in conical flasks and sterilized by high-pressure steam for 20min, and a proper amount of the mixture is poured into a sterilized culture dish to prepare the nutrient agar plate.

(3) Preparing an air breathing cathode: the air breathing cathode consists of GDL, CL and steel mesh serving as a current collector. CL consists of Carbon Black (CB), graphite and PTFE emulsion as binder (60 wt.%), in a ratio of 1 g: 5 g: 0.57 mL. GDL consisted of CB and PTFE emulsion (60 wt.%) in a ratio of 4 g: 6 mL.

(4)H₂O₂In situ generation of (a): h₂O₂The production of (a) was carried out in a two-electrode system in a single-chamber reactor (28mL, 3cm diameter and 4cm length), with a Pt electrode for the anode and a carbon black/graphite air breathing cathode for the cathode. At 20mAcm^-2，50mM Na₂SO₄(pH 7) in situ production of H as an electrolyte solution₂O₂. H accumulated in 1H₂O₂(2044±126mg L^-1) Atomized and sprayed into the disinfection device.

(5) Set control group experiment: setting single UV disinfection, UV/H₂O₂Sterilizing groups using UV lamps of different powers in combination with atomization H₂O₂Or H₂O₂And (3) disinfecting the solution, opening the dish cover of the nutrient agar plate after disinfection, placing the nutrient agar plate in devices with different sizes, exposing the nutrient agar plate in air for 5min, covering the dish cover, turning the plate upside down, placing the plate in a constant-temperature incubator at 37 ℃ for culturing for 24h, and counting the number of colonies.

Compared with the prior reports, the beneficial effect of the invention is embodied in that H is generated in situ₂O₂The green method avoids the synthesis of H by the traditional process₂O₂Problems such as large energy input, complicated steps, more waste generated and the like in the Anthraquinone Oxidation (AO) process are solved, and H is avoided₂O₂Potential hazards and additional costs associated with storage and handling during transportation. By mixing H₂O₂Atomizing and spraying into air to increase the contact surface of hydroxyl free radical and bacteria in airAnd thus higher sterilization. At the same time, UV/H compared to other AOP₂O₂In the process, hydroxyl free radicals (. OH) play a main role in inactivating microorganisms, and other ions are not introduced in the process, so that the sterilization effect is better.