阅读说明：本技术 一种荧光检测系统 (Fluorescence detection system ) 是由 朱灵 杨柯 花昌义 潘井宇 李志刚 朱灿灿 赵俊 汪磊 刘勇 于 2021-08-18 设计创作，主要内容包括：本发明公开了一种荧光检测系统,包括底座以及固定在底座上的旋转滤光盘模块、激发光源模块、光纤导光模块和荧光接收模块；所述旋转滤光盘模块包括支架、激发旋转滤光盘、发射旋转滤光盘、激发滤光片和发射滤光片,所述激发光源模块的输出端靠近激发滤光片设置；所述光纤导光模块包括多组光纤、激发聚焦透镜组件和发射聚焦透镜组件。本发明的优点在于,该检测系统能够实现至少两种不同波段的荧光检测,提高该系统的检测通量；同时还能够有效降低激发光源模块与检测试剂的距离对荧光激发和收集效率的影响,提高荧光激发和收集的效率,提高该系统的检测精度。(The invention discloses a fluorescence detection system, which comprises a base, and a rotary optical filter module, an excitation light source module, an optical fiber light guide module and a fluorescence receiving module which are fixed on the base; the rotary filter disc module comprises a bracket, an excitation rotary filter disc, an emission rotary filter disc, an excitation optical filter and an emission optical filter, and the output end of the excitation light source module is arranged close to the excitation optical filter; the optical fiber light guide module comprises a plurality of groups of optical fibers, an excitation focusing lens assembly and a transmitting focusing lens assembly. The invention has the advantages that the detection system can realize the fluorescence detection of at least two different wave bands, and the detection flux of the system is improved; meanwhile, the influence of the distance between the excitation light source module and the detection reagent on the fluorescence excitation and collection efficiency can be effectively reduced, the fluorescence excitation and collection efficiency is improved, and the detection precision of the system is improved.)

1. A fluorescence detection system, characterized by: the device comprises a base, and a rotary optical filter disc module, an excitation light source module, an optical fiber light guide module and a fluorescence receiving module which are fixed on the base;

the rotary filter disc module comprises a support, an excitation rotary filter disc, an emission rotary filter disc, excitation optical filters and emission optical filters, wherein the support is fixed on a base;

the output end of the excitation light source module is arranged close to the excitation optical filter;

the optical fiber light guide module comprises a plurality of groups of optical fibers, an excitation focusing lens assembly and a transmission focusing lens assembly, one end of each group of optical fibers is an emission end, the other end of each group of optical fibers is divided into two parts which are respectively an excitation end and a collection end, the excitation end of each group of optical fibers is connected with the excitation focusing lens assembly, the excitation focusing lens assembly is fixed on the support in the circumferential direction of the center of the excitation rotary filter disc, the diameter of the circumferential direction of the excitation focusing lens assembly is the same as the diameter of the circumferential direction of the excitation filter disc, the collection end of each group of optical fibers is connected with the transmission focusing lens assembly, the transmission focusing lens assembly is fixed on the support in the circumferential direction of the center of the emission rotary filter disc, and the diameter of the circumferential direction of the transmission focusing lens assembly is the same as the diameter of the circumferential direction of the emission filter disc;

the fluorescence receiving module is fixed on the bracket close to one side of the emission rotary filter disc.

2. A fluorescence detection system according to claim 1, wherein: the excitation light source module comprises an excitation light source control plate and excitation light sources, the excitation light source control plate is fixed on the excitation rotary filter disc, and the excitation light sources corresponding to the excitation light filters in position and quantity are fixed on the excitation light source control plate.

3. A fluorescence detection system according to claim 2, wherein: the excitation light source is LED lamp beads with different wavelengths.

4. A fluorescence detection system according to claim 1, wherein: the fluorescence receiving module is a photomultiplier tube.

5. A fluorescence detection system according to claim 1, wherein: and the base is also fixedly provided with a control system, and the excitation light source module and the fluorescence receiving module are both connected with the control system.

6. A fluorescence detection system according to claim 5, wherein: the base is further fixed with a conducting ring support, the conducting ring support is fixed with a conducting ring, and the control system is connected with the excitation light source module through the conducting ring.

7. A fluorescence detection system according to claim 1, wherein: the filter disc type laser device is characterized by further comprising a channel switching module fixed on the base, wherein the output end of the channel switching module penetrates through the support and is connected with the excitation rotary filter disc and the emission rotary filter disc, and the channel switching module is driven to enable the excitation rotary filter disc and the emission rotary filter disc to synchronously rotate.

8. A fluorescence detection system according to claim 7, wherein: the passageway switches the module and includes driving motor, driving shaft, driven shaft, action wheel, follows driving wheel and belt, driving motor fixes on the base, driving motor's output is connected the driving shaft, driving motor's one end is kept away from to the driving shaft runs through support and coaxial coupling arouse rotatory filter disc, support and coaxial coupling are run through to driven shaft one end the rotatory filter disc of transmission, fix on the driving shaft the action wheel, fix on the driven shaft follow the driving wheel, pass through the belt between action wheel and the follow driving wheel and be connected.

9. A fluorescence detection system according to claim 8, wherein: and one end of the driven shaft, which is far away from the emission rotary filter disc, is also connected with an encoder.

10. A fluorescence detection system according to claim 8, wherein: the driving motor is a progressive motor.

Technical Field

The invention relates to the technical field of medical instruments, in particular to a fluorescence detection system.

Background

In recent years, various infectious diseases seriously harm human health, and bring great influence to social economy and production and life of people. Therefore, the development of rapid and efficient detection of infectious diseases is of great significance to human life health and social economic development.

Among the methods for detecting infectious diseases, the Nucleic acid detection technology (Nucleic acid testing/NAT) is becoming the mainstream method for detecting infectious diseases because of its advantages of high detection speed, high sensitivity and good specificity. A complete NAT process typically includes three steps: nucleic acid extraction, nucleic acid amplification and result detection. The qualitative or quantitative detection of nucleic acid is realized by a method of labeling with fluorescent dye or probe aiming at the nucleic acid amplification result. The performance of the fluorescence detection system directly determines the nucleic acid detection result.

In current nucleic acid analysis instruments, the fluorescence detection light path usually adopts an orthogonal, oblique or transmissive optical structure, and the orthogonal optical structure is most commonly used. Taking an orthogonal fluorescence detection path as an example, the main devices comprise: excitation light source, collimating lens group, excitation and emission filter, dichroic mirror and photoelectric detector. The broadband light emitted by the excitation light source is focused and collimated by the collimating lens group, then passes through the excitation filter to obtain excitation light with a required waveband, is reflected by the dichroic mirror and irradiates on reaction liquid in the detection chip arranged on the excitation surface, and fluorescence is excited. The fluorescence is received by the photoelectric detector through the dichroic mirror and the emission filter, and the amplification result of the nucleic acid is obtained by analyzing the strength of the fluorescence signal received by the photoelectric detector.

The fluorescence detection optical paths with these optical structures lead to that the excitation light source and the photodetector are far away from the detection chip when the current nucleic acid analysis instrument is designed, which not only increases the volume of the instrument and the complexity of the structural design, but also as for the excitation optical path, because the excitation light source has a certain divergence angle, although the collimating lens group is designed, the intensity and uniformity of the excitation light on the detection chip are still difficult to ensure; for the emission light path, although there is an optical filter to filter out stray light, the photodetector is still very susceptible to the stray light when collecting the fluorescence signal. These problems result in inefficient fluorescence excitation and collection of the instrument, which affects the detection accuracy of the instrument. In addition, the flux of the highest detection signal of the current nucleic acid analysis instrument is low, which also limits the application scenario of the nucleic acid analysis instrument.

Patent document CN201911111985.4 discloses an optical fiber sensing microfluidic chip nucleic acid amplification in-situ real-time detection system and method, the detection system includes a first optical fiber sensor and a second optical fiber sensor, the first optical fiber sensor transmits excitation light emitted by an excitation light source to the microfluidic chip for fluorescence excitation; the second optical fiber sensor collects fluorescence at the microfluidic chip and transmits the fluorescence to the spectrum acquisition processing display module, and changes of fluorescence signals are displayed in real time.

Patent document CN201911061352.7 discloses a nucleic acid amplification instrument, which comprises a shooting component and a fluorescence module, wherein the shooting component comprises a lens and a camera and is used for shooting after nucleic acid amplification; the fluorescence module adopts an orthogonal fluorescence detection structure and comprises an excitation light source component and a rotary filter wheel; the rotary optical filter wheel comprises an optical filter assembly turntable and a turntable power device, the turntable power device drives the optical filter assembly turntable to rotate to switch the fluorescence channel, and the fluorescence channel can be excited aiming at more than six different fluorescences, so that the detection flux of the nucleic acid amplification instrument is improved.

The first patent uses optical fibers to transmit excitation light and emission light, so that the influence of the distance between the excitation light source and the photoelectric detector and the detection chip on the fluorescence excitation and collection efficiency is reduced, and the detection precision of the instrument is improved; but it cannot excite different fluorescence simultaneously, and the detection signal flux is low. In the second patent, a rotary filter assembly turntable and a turntable power device are used for forming a rotary filter wheel, so that excitation of more than six different types of fluorescence is realized, and the detection flux of an instrument is improved; however, the excitation light source assembly and the camera provided with the lens, which are arranged on the left side of the rotary filter wheel, are far away from the excitation surface, so that the fluorescence excitation and collection efficiency is low, and the detection precision of the instrument is influenced.

Disclosure of Invention

The invention aims to solve the technical problem of how to improve the detection flux and the detection precision of a fluorescence detection system.

In order to solve the technical problems, the invention provides the following technical scheme:

a fluorescence detection system comprises a base, and a rotary optical filter module, an excitation light source module, an optical fiber light guide module and a fluorescence receiving module which are fixed on the base.

The rotary filter disc module comprises a support, a rotary filter disc, a transmitting rotary filter disc, an exciting optical filter and a transmitting optical filter, wherein the support is fixed on the base, the support is fixedly provided with the rotary filter disc and the transmitting rotary filter disc which can rotate, at least two exciting optical filters are arranged on the rotary filter disc, the exciting optical filters are arranged in a circumferential direction in the center of the rotary filter disc, the transmitting optical filters with the same number as the exciting optical filters are arranged on the transmitting rotary filter disc, and the transmitting optical filters are arranged in a circumferential direction in the center of the transmitting rotary filter disc.

The output end of the excitation light source module is arranged close to the excitation filter.

The optical fiber light guide module comprises a plurality of groups of optical fibers, an excitation focusing lens assembly and a transmission focusing lens assembly, one end of each group of optical fibers is an emission end, the other end of each group of optical fibers is divided into two parts which are respectively an excitation end and a collection end, the excitation end of each group of optical fibers is connected with the excitation focusing lens assembly, the excitation focusing lens assembly is fixed on the support in the circumferential direction of the center of the excitation rotary filter disc, the diameter of the excitation focusing lens assembly in the circumferential direction is the same as the diameter of the excitation filter disc in the circumferential direction, the collection end of each group of optical fibers is connected with the transmission focusing lens assembly, the transmission focusing lens assembly is fixed on the support in the circumferential direction of the center of the transmission rotary filter disc in the circumferential direction, and the diameter of the transmission focusing lens assembly in the circumferential direction is the same as the diameter of the transmission filter disc in the circumferential direction.

The fluorescence receiving module is fixed on the bracket close to one side of the emission rotary filter disc.

The detection system can realize the fluorescence detection of at least two different wave bands by arranging at least two excitation light filters on the excitation rotary filter disc and arranging emission light filters on the corresponding emission rotary filter disc, thereby improving the detection flux of the system; meanwhile, the excitation light is transmitted and the fluorescence is collected through the optical fiber light guide module, so that the influence of the distance between the excitation light source module and the detection reagent on the fluorescence excitation and collection efficiency can be effectively reduced, the fluorescence excitation and collection efficiency is improved, and the detection precision of the system is improved.

Preferably, the excitation light source module comprises an excitation light source control board and excitation light sources, the excitation light source control board is fixed on the excitation rotary filter disc, and the excitation light sources corresponding to the excitation optical filters in position and quantity are fixed on the excitation light source control board.

Preferably, the excitation light source is LED lamp beads with different wavelengths.

Preferably, the fluorescence receiving module is a photomultiplier tube.

Preferably, a control system is further fixed on the base, and the excitation light source module and the fluorescence receiving module are both connected with the control system.

Preferably, a conducting ring support is further fixed on the base, a conducting ring is fixed on the conducting ring support, and the control system is connected with the excitation light source module through the conducting ring.

Preferably, the optical filter device further comprises a channel switching module fixed on the base, an output end of the channel switching module penetrates through the bracket and is connected with the excitation rotary filter disk and the emission rotary filter disk, and the channel switching module is driven to enable the excitation rotary filter disk and the emission rotary filter disk to synchronously rotate.

Preferably, the passageway switching module includes driving motor, driving shaft, driven shaft, action wheel, follows driving wheel and belt, driving motor fixes on the base, driving motor's output is connected the driving shaft, driving motor's one end is kept away from to the driving shaft runs through support and coaxial coupling arouse rotatory filter disc, support and coaxial coupling are run through to driven shaft one end the rotatory filter disc of transmission, fix on the driving shaft the action wheel, fix on the driven shaft follow the driving wheel, pass through the belt between action wheel and the follow driving wheel and be connected.

Preferably, the end of the driven shaft far away from the emission rotary filter disc is also connected with an encoder.

Preferably, the driving motor is a step motor.

Compared with the prior art, the invention has the beneficial effects that:

1. the detection system can realize the fluorescence detection of at least two different wave bands by arranging at least two excitation light filters on the excitation rotary filter disc and arranging emission light filters on the corresponding emission rotary filter disc, thereby improving the detection flux of the system; meanwhile, the excitation light is transmitted and the fluorescence is collected through the optical fiber light guide module, so that the influence of the distance between the excitation light source module and the detection reagent on the fluorescence excitation and collection efficiency can be effectively reduced, the fluorescence excitation and collection efficiency is improved, and the detection precision of the system is improved.

2. Through the arrangement of the LED lamp beads with different wavelengths, each LED lamp bead corresponds to one fluorescence channel, and the bandwidths of the six LED lamp beads are narrow, so that the fluorescence crosstalk between adjacent fluorescence channels can be effectively reduced; each LED lamp bead has the maximum luminous intensity on the corresponding fluorescence channel, and the complexity of the design of the excitation light source control circuit is reduced by selecting the LED lamp beads with small power.

3. Through the setting of passageway switching module, realize arousing the position synchronous rotation of rotatory filter disc and the rotatory filter disc of transmission, not only realize the synchronous switching of fluorescence passageway for when switching fluorescence passageway at every turn, all have the fluorescence passageway of four different wave bands to gather fluorescence simultaneously, still improved the fluorescence detection efficiency of system.

4. The control system is connected with the excitation light source control board through the conducting ring, so that the integration degree of the system can be improved, and efficient fluorescence channel switching can be performed.

Drawings

FIG. 1 is a schematic diagram of a fluorescence detection system according to an embodiment of the present invention;

FIG. 2 is a front view of an embodiment of the present invention;

fig. 3 is a partial structural diagram of an embodiment of the invention.

Detailed Description

In order to facilitate the understanding of the technical solutions of the present invention for those skilled in the art, the technical solutions of the present invention will be further described with reference to the drawings attached to the specification.

In this application, unless expressly stated or limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can include, for example, fixed connections, removable connections, or integral parts; the connection can be mechanical connection, electrical connection or communication; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.

In this application, unless explicitly stated or limited otherwise, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, "a plurality" means two or more unless specifically limited otherwise.

Referring to fig. 1 to 3, the present embodiment discloses a fluorescence detection system, which includes a base 1, and a rotary filter module 2, an excitation light source module 3, an optical fiber light guide module 4, a fluorescence receiving module 5, a channel switching module 6, and a control system 7 on the base 1.

The rotary filter disk module 2 comprises a support 21, an excitation rotary filter disk 22, an emission rotary filter disk 23, excitation filters 24 and emission filters 25, wherein the support 21 is fixed on the base 1, in this embodiment, the cross section of the support 21 is in a U shape, the excitation rotary filter disk 22 is rotatably fixed on the right side of the support 21, the emission rotary filter disk 23 is rotatably fixed on the inner side of the support 21, the excitation rotary filter disk 22 is provided with at least two excitation filters 24, the excitation filters 24 are circumferentially arranged at the center of the excitation rotary filter disk 22, the emission rotary filter disk 23 is provided with emission filters 25 with the same number as the excitation filters 24, the emission filters 25 are circumferentially arranged at the center of the emission rotary filter disk 23, and each fluorescence band is provided with a group of corresponding excitation filters 24 and emission filters 25, in the embodiment, the number of the excitation filter 24 and the emission filter 25 is 6, and the optical parameters of each filter are different, corresponding to six sets of fluorescence bands.

The excitation light source module 3 comprises an excitation light source control plate 31 and excitation light sources 32, the excitation light source control plate 31 is fixed on the excitation rotary filter disc 22, the excitation light source control plate 31 is connected with the control system 7, the excitation light sources 32 corresponding to the positions and the number of the excitation light filters 24 are fixed on the excitation light source control plate 31, in this embodiment, the excitation light sources 32 are LED lamp beads with different wavelengths, each LED lamp bead corresponds to one fluorescence channel, the bandwidths of the six LED lamp beads are narrow, and the fluorescence crosstalk between adjacent fluorescence channels can be effectively reduced; each LED lamp bead has the maximum luminous intensity on the corresponding fluorescence channel, and the complexity of the design of the excitation light source control circuit is reduced by selecting the LED lamp beads with small power.

The optical fiber light guide module 4 comprises a plurality of sets of optical fibers 41, an excitation focusing lens assembly 42 and an emission focusing lens assembly 43, which, in this embodiment, the detection system is used for detecting the quadruple pipe 8, the quadruple pipe 8 is fixed on the heat sink sheet 9, the side surface of the heat sink sheet 9 is provided with a small hole, correspondingly, 4 groups of optical fibers 41 are arranged, one end of each group of optical fibers 41 is a transmitting end 411, the other end of each group of optical fibers 41 is divided into two parts which are respectively an exciting end 412 and a collecting end 413, the four transmitting ends 411 extend into the small holes of the heat sink sheet, the exciting end 411 of each group of optical fibers 41 is connected with an exciting and focusing lens component 42, the excitation focusing lens assembly 42 is circumferentially fixed to the support 21 at the center of the excitation rotating filter wheel 22, the diameter of the excitation focusing lens assembly 42 arranged in the circumferential direction is the same as the diameter of the excitation filter 24 arranged in the circumferential direction, so that the excitation filter 24 can be aligned with the excitation focusing lens assembly 42 after the excitation rotary filter disc 22 rotates. Similarly, the collecting end 413 of each group of optical fibers 41 is connected to the emission focusing lens assembly 43, the emission focusing lens assembly 43 is circumferentially fixed on the support 21 at the center of the emission rotary filter disc 23, and the diameter of the emission focusing lens assembly 43 circumferentially set is the same as the diameter of the emission filter 25 circumferentially set, so that the emission filter 25 can be aligned to the emission focusing lens assembly 43 after the emission rotary filter disc 23 rotates.

The fluorescence receiving module 5 is fixed on the bracket 21 close to one side of the excitation rotary filter disc 22, and the fluorescence receiving module 5 is electrically connected with the control system 7. In this embodiment, the fluorescence receiving module 5 is a photomultiplier tube with the number corresponding to the number of the quadruple tubes 8.

Specifically, the fluorescence collected by the four emission focusing lens groups 43 is received by the four photomultiplier tubes at the rear through the four optical filters 51 on the emission rotary filter disk 23, and the received fluorescence is converted into a voltage signal by the four photomultiplier tubes 7 and sent to the control system 7, and a fluorescence intensity value is obtained through program analysis.

The channel switching module 6 comprises a driving motor 61, a driving shaft 62, a driven shaft 63, a driving wheel 64, a driven wheel 65, a belt 66 and an encoder 67, in this embodiment, the driving motor 61 is a stepping motor, the driving motor 61 is fixed on the base 1, the output end of the driving motor 61 is connected with the driving shaft 62, one end of the driving shaft 62 far away from the driving motor 61 penetrates through the bracket 21 and is coaxially connected with the excitation rotary filter disc 22, one end of the driven shaft 63 penetrates through the bracket 21 and is coaxially connected with the emission rotary filter disc 23, the driving wheel 64 is fixed on the driving shaft 62, the driven wheel 65 is fixed on the driven shaft, the driving wheel 64 is connected with the driven wheel 65 through a belt 66, one end of the driven shaft 63, which is far away from the emission rotary filter disc 23, is connected with the encoder 67, and the driving motor 61 and the encoder 67 are both connected with the control system 7.

Specifically, encoder 67 is used for recording driving motor 61's rotation angle to return angle numerical value for control system 7, control system 7 is according to angle numerical value control driving motor 61's rotation, angle feedback through encoder 67, driving motor 61 realizes arousing the position synchronous rotation of rotatory filter disc 22 and the rotatory filter disc 23 of transmission, not only realize the synchronous switch-over of fluorescence passageway, make when switching fluorescence passageway at every turn, all have the fluorescence passageway of four different wave bands to gather fluorescence simultaneously, the fluorescence detection efficiency of system has still been improved.

Further, a conducting ring support 10 is further fixed on the base 1, a conducting ring (not labeled in the figure) is fixed on the conducting ring support 10, and the control system 7 is connected with the excitation light source control board 31 through the conducting ring, so that the integration degree of the system can be improved, and efficient fluorescence channel switching can be performed.

The detection system can realize the fluorescence detection of six different wave bands by arranging six excitation light filters 24 on the excitation rotary filter disc 22 and six emission light filters 25 on the corresponding emission rotary filter disc 23, thereby improving the detection flux of the system; meanwhile, the excitation light is transmitted and the fluorescence is collected through the optical fiber light guide module 4, so that the influence of the distance between the excitation light source module 3 and the quadruple tube 8 on the fluorescence excitation and collection efficiency can be effectively reduced, the fluorescence excitation and collection efficiency is improved, and the detection precision of the system is improved.

The working principle of the embodiment is as follows: the excitation light source control board 31 is controlled by the control system 7, the excitation light source 32 is further controlled to be opened, excitation light generated by the excitation light source 32 penetrates through the excitation optical filter 24 and is transmitted to the emission end 411 by the excitation focusing lens assembly 42 and the excitation end 412, the excitation light output by the emission end 411 excites fluorescence of a detection reagent in the quadruple tube 8, then the fluorescence is transmitted to the collection end 413, the collection end 413 transmits the collected fluorescence to the emission focusing lens assembly 43, the fluorescence is received by the four photomultiplier tubes behind through the four optical filters 51 on the emission rotating filter disc 23, the received fluorescence is converted into voltage signals by the four photomultiplier tubes 7, the voltage signals are transmitted to the control system 7, and a fluorescence intensity value is obtained through program analysis.

Then the control system 7 controls the rotation of the driving motor 61, the positions of the excitation rotary filter disk 22 and the emission rotary filter disk 23 synchronously rotate through the driving shaft 52 and the driven shaft 63, the excitation light source 32 is driven to rotate, the excitation light source 32 is aligned with the next excitation rotary filter disk 22, and the detection steps are repeated, so that when the fluorescence channel is switched each time, four fluorescence channels with different wave bands simultaneously collect fluorescence.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein, and any reference signs in the claims are not intended to be construed as limiting the claim concerned.

The above-mentioned embodiments only represent embodiments of the present invention, and the protection scope of the present invention is not limited to the above-mentioned embodiments, and it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the concept of the present invention, and these embodiments are all within the protection scope of the present invention.