阅读说明：本技术 一种细胞培养系统及培养方法 (Cell culture system and culture method ) 是由 施忠林 于 2021-09-04 设计创作，主要内容包括：本发明涉及细胞培养领域,更具体的说是一种细胞培养系统及培养方法。一种细胞培养系统,包括有动物组织处理装置,所述动物组织处理装置将动物组织块切出多个竖刀槽和一个横刀槽。所述动物组织处理装置包括有处理板、升降条、L形柱和竖刀,升降条的前后两端都设置有L形柱,两个L形柱的上部都设置有竖刀,两个竖刀平行设置,两个竖刀都设置在处理板的上侧,两个竖刀前后错位设置。方法包括有以下步骤：S1：取动物组织块；S2：使用动物组织处理装置将动物组织块切出多个竖刀槽和一个横刀槽；S3：用胰蛋白酶处理动物组织,使动物组织分散成多个单细胞；S4：对动物细胞进行培养。(The invention relates to the field of cell culture, in particular to a cell culture system and a cell culture method. A cell culture system includes an animal tissue processing apparatus that cuts a plurality of vertical knife channels and a horizontal knife channel into an animal tissue piece. Animal tissue processing apparatus is including handling board, lifter bar, L shape post and perpendicular sword, and both ends all are provided with L shape post around the lifter bar, and the upper portion of two L shape posts all is provided with perpendicular sword, and two perpendicular sword parallel arrangement, two perpendicular swoves all set up the upside at the processing board, and dislocation set around two perpendicular swoves. The method comprises the following steps: s1: taking an animal tissue block; s2: cutting a plurality of vertical knife grooves and a transverse knife groove on an animal tissue block by using an animal tissue processing device; s3: treating animal tissue with trypsin to disperse the animal tissue into a plurality of single cells; s4: the animal cells are cultured.)

1. A cell culture system comprising an animal tissue processing device, characterized in that: the animal tissue processing device cuts a plurality of vertical knife grooves and a transverse knife groove on an animal tissue block.

2. A cell culture system according to claim 1, wherein: animal tissue processing apparatus is including handling board (101), lifter bar (301), L shape post (303) and perpendicular sword (304), and both ends all are provided with L shape post (303) around lifter bar (301), and the upper portion of two L shape posts (303) all is provided with perpendicular sword (304), and two perpendicular sword (304) parallel arrangement, two perpendicular sword (304) all set up the upside at handling board (101), and dislocation set around two perpendicular sword (304).

3. A cell culture system according to claim 2, wherein: the animal tissue treatment device further comprises fastening screws (302), the lower part of each L-shaped column (303) is transversely connected to the lifting bar (301) in a sliding mode, the front end and the rear end of the lifting bar (301) are connected with the fastening screws (302) in a threaded mode, and the fastening screws (302) are pressed against the corresponding L-shaped columns (303).

4. A cell culture system according to claim 3, wherein: the animal tissue processing device further comprises top strips (201) and needle rods (208), the left end and the right end of the processing plate (101) are respectively provided with the top strips (201), the lower sides of the two top strips (201) are respectively provided with the plurality of needle rods (208) from front to back, and the top strips (201) can move up and down and left and right relative to the processing plate (101).

5. A cell culture system according to claim 4, wherein: the animal tissue treatment device further comprises lifting columns (205) and sliders (206), wherein the vertical lifting columns (205) are arranged at the front end and the rear end of the lower side of each top strip (201), the sliders (206) are vertically and slidably connected to the lifting columns (205), and the two sliders (206) on the lower side of the same top strip (201) are respectively and transversely slidably connected to the front edge and the rear edge of the treatment plate (101).

6. A cell culture system according to claim 5, wherein: the animal tissue processing device further comprises a separation blade (207), the lower end of each lifting column (205) is fixedly connected with the separation blade (207), each lifting column (205) is sleeved with a compression spring, and the compression springs are located between the corresponding sliding blocks (206) and the separation blades (207).

7. A cell culture system according to claim 6, wherein: animal tissue processing apparatus still includes well seat (102), telescopic link I (103) and V pole (209), fixedly connected with V pole (209) between the downside of two sliders (206) of same top strip (201) downside, seat (102) in the downside middle part fixedly connected with of processing plate (101), the equal fixedly connected with telescopic link I (103) in both sides of well seat (102), the expansion end difference fixed connection of two telescopic link I (103) is on two V poles (209).

8. A cell culture system according to claim 7, wherein: the animal tissue treatment device further comprises threaded columns (202), pressure rods (203) and ribs (204), wherein the pressure rods (203) are arranged on the lower side of each top strip (201), the threaded columns (202) are fixedly connected to the front end and the rear end of the upper side of the pressure rods (203), the two threaded columns (202) on the pressure rods (203) are inserted into the front end and the rear end of each corresponding top strip (201) respectively, nuts are connected to the upper portions of the threaded columns (202) in a threaded mode, the ribs (204) are arranged on the lower side of the pressure rods (203), and the pressure rods (203) are located below the needle rods (208).

9. A cell culture system according to claim 8, wherein: the animal tissue treatment device further comprises a flat knife (501), the flat knife (501) is arranged on the right side of the treatment plate (101), and the flat knife (501) can move up and down, left and right relative to the treatment plate (101).

10. A method of culturing cells in a cell culture system according to claim 9, comprising the steps of:

s1: taking an animal tissue block;

s2: cutting a plurality of vertical knife grooves and a transverse knife groove on an animal tissue block by using an animal tissue processing device;

s3: treating animal tissue with trypsin to disperse the animal tissue into a plurality of single cells;

s4: the animal cells are cultured.

Technical Field

The invention relates to the field of cell culture, in particular to a cell culture system and a cell culture method.

Background

Cell culture fibre membrane support frame, cell culture support and cell culture device that patent number is CN201620066733.X discloses a cell culture fibre membrane support frame, cell culture support and cell culture device, relates to medical treatment consumptive material technical field. The invention aims to solve the problems of low inoculation efficiency and high culture and detection difficulty in cell culture by adopting a cell culture fibrous membrane in the prior art. The utility model discloses a cell culture fibrous membrane support frame is used for supporting cell culture fibrous membrane, cell culture fibrous membrane is used for cell culture, the support frame is formed with the support mouth, support the mouth with cell culture fibrous membrane matches, it is fixed to support the mouth and be used for supporting the border a week of cell culture fibrous membrane. The utility model discloses a cell culture fibrous membrane support frame, cell culture support and cell culture device can be used to improve cell inoculation efficiency and reduce cell culture and detect the degree of difficulty. However, this patent does not facilitate adequate handling of animal tissue by trypsin, which disperses the animal tissue into a plurality of single cells

Disclosure of Invention

The animal tissue processing device provided by the invention has the beneficial effects that a plurality of vertical cutter grooves and a transverse cutter groove are cut in an animal tissue block, so that animal tissues can be fully processed by trypsin, and the animal tissues are dispersed into a plurality of single cells.

A cell culture system includes an animal tissue processing apparatus that cuts a plurality of vertical knife channels and a horizontal knife channel into an animal tissue piece.

Animal tissue processing apparatus is including handling board, lifter bar, L shape post and perpendicular sword, and both ends all are provided with L shape post around the lifter bar, and the upper portion of two L shape posts all is provided with perpendicular sword, and two perpendicular sword parallel arrangement, two perpendicular swoves all set up the upside at the processing board, and dislocation set around two perpendicular swoves.

The animal tissue treatment device further comprises fastening screws, the lower portion of each L-shaped column is connected to the lifting strip in a transverse sliding mode, the front end and the rear end of the lifting strip are connected with the fastening screws in a threaded mode, and the fastening screws abut against the corresponding L-shaped columns.

The animal tissue treatment device further comprises top strips and needle rods, wherein the top strips are arranged at the left end and the right end of the treatment plate, a plurality of needle rods are arranged on the lower sides of the two top strips from front to back, and the top strips can move up and down and left and right relative to the treatment plate.

The animal tissue treatment device further comprises lifting columns and sliding blocks, the front end and the rear end of the lower side of each top strip are provided with vertical lifting columns, the lifting columns are vertically and slidably connected with the sliding blocks, and the two sliding blocks on the lower side of the same top strip are respectively and transversely and slidably connected to the front edge and the rear edge of the treatment plate.

The animal tissue processing device further comprises separation blades, the separation blades are arranged at the lower ends of the lifting columns, compression springs are sleeved on the lifting columns, and the compression springs are located between the corresponding sliding blocks and the separation blades.

Animal tissue processing apparatus is still including well seat, telescopic link I and V pole, is provided with the V pole between the downside of two sliders of same top strip downside, and the downside middle part of handling the board is provided with well seat, and the both sides of well seat all are provided with telescopic link I, and the expansion end of two telescopic link I sets up respectively on two V poles.

The animal tissue treatment device further comprises threaded columns, pressure rods and convex ribs, the pressure rods are arranged on the lower side of each top strip, the threaded columns are arranged at the front end and the rear end of the upper side of each pressure rod, the two threaded columns on the pressure rods are inserted into the front end and the rear end of the corresponding top strip respectively, nuts are connected to the upper portions of the threaded columns in a threaded mode, the convex ribs are arranged on the lower sides of the pressure rods, and the pressure rods are located below the needle rods.

The animal tissue treatment device also comprises a flat knife, wherein the flat knife is arranged on the right side of the treatment plate, and the flat knife can move up and down, left and right relative to the treatment plate.

A method for culturing cells by using a cell culture system comprises the following steps:

s1: taking an animal tissue block;

s2: cutting a plurality of vertical knife grooves and a transverse knife groove on an animal tissue block by using an animal tissue processing device;

s3: treating animal tissue with trypsin to disperse the animal tissue into a plurality of single cells;

s4: the animal cells are cultured.

Drawings

The invention is described in further detail below with reference to the accompanying drawings and specific embodiments.

FIG. 1 is a schematic diagram of a cell culture system;

FIG. 2 is a first schematic view of the structure of the animal tissue processing device;

FIG. 3 is a schematic structural view II of the animal tissue processing device;

FIG. 4 is a first schematic view of a processing plate;

FIG. 5 is a second schematic view of the processing plate;

FIG. 6 is a schematic structural view of the lifter bar;

FIG. 7 is a schematic structural view of the bottom frame;

FIG. 8 is a schematic structural view of a flat blade;

FIG. 9 is a first schematic structural view of an animal tissue body;

fig. 10 is a second schematic structural view of an animal tissue body.

In the figure: a processing plate 101; a middle seat 102; a telescopic rod I103; a top strip 201; a threaded post 202; a pressure lever 203; a rib 204; a lifting column 205; a slider 206; a stopper 207; a needle bar 208; a V-bar 209; a lifting bar 301; a fastening screw 302; an L-shaped post 303; a vertical knife 304; a bottom frame 401; a telescopic rod II 402; a slide sheet 403; a lead screw 404; a motor I405; a telescopic rod II 406; a trapezoidal rail 407; a side bar 408; a center pillar 409; a flat blade 501; a motor base 502; a motor II 503; a friction wheel 504; a friction lever 505; an animal tissue body 601; a lateral knife channel 602; vertical knife slots 603.

Detailed Description

A cell culture system includes an animal tissue processing apparatus that cuts a plurality of vertical knife slots and a lateral knife slot into an animal tissue piece.

Referring to fig. 1, an embodiment of the present invention is provided, which solves the main technical problem of facilitating the dispersion of animal tissue cells, and further,

because animal tissue processing apparatus can cut out a plurality of vertical sword grooves and a horizontal sword groove with the animal tissue piece, and then cut out the animal tissue in a plurality of vertical sword grooves and a horizontal sword groove and put in the trypsin solution after, in the easy access animal tissue of trypsin, and then conveniently disperse animal tissue cell, be convenient for cultivate animal cell in next step.

Referring to fig. 1-10, an embodiment of the present invention is provided, which solves the main technical problem of cutting a plurality of vertical knife slots on animal tissue, and further,

because animal tissue processing apparatus includes processing plate 101, lifter bar 301, L shape post 303 and vertical sword 304, the both ends of lifter bar 301 all are provided with L shape post 303, the upper end of every L shape post 303 has all set up vertical sword 304, be parallel arrangement between two vertical sword 304, two vertical sword 304 all are located the upside of processing plate 101, after the upside of processing plate 101 is spread animal tissue to the tie, can move tangential animal tissue down with two vertical sword 304, remove two vertical sword 304 about again, cut out a plurality of vertical sword grooves on animal tissue, dislocation set around two vertical sword 304, and then can not cut off animal tissue.

Referring to fig. 1-10, an embodiment of the present invention is provided, which solves the main technical problem of adjusting the distance between two adjacent vertical knife slots, and further,

since the animal tissue processing apparatus further includes the fastening screw 302, the L-shaped column 303 can slide relative to the lifting bar 301 to adjust the interval between the two vertical knives 304, and thus the interval between the two vertical knife grooves which are cut out adjacently.

Referring to fig. 1-10, an embodiment of the present invention is provided to stretch animal tissues from left to right to prevent the animal tissues from rolling up, and further,

because animal tissue processing apparatus still includes top strip 201 and needle bar 208, both ends all are equipped with top strip 201 about processing board 101, and the downside of two top strips 201 all is provided with a plurality of needle bars 208, and top strip 201 can be relatively handle board 101 from top to bottom, move about, and then can make a plurality of needle bars 208 of top strip 201 downside insert the tip of animal tissue, then two top strips 201 keep away from each other and slide, and then stretch the animal tissue from a left side to the right side, prevent that animal tissue from rolling up.

Referring to fig. 1-10, an embodiment of the present invention is provided, which solves the main technical problem of adjusting the heights of the plurality of needle bars 208 on the top strip 201, so that the plurality of needle bars 208 are inserted into the end of the animal tissue, and further,

because animal tissue processing apparatus still includes lift post 205 and slider 206, every top strip 201 downside all is provided with vertical lift post 205, vertical sliding connection has slider 206 on the lift post 205, two sliders 206 transversely sliding connection are at the front and back edge of handling board 101 respectively, and then can drive two lift posts 205 and a plurality of needle bars 208 on the top strip 201 and move about when two sliders 206 slide left and right, and a plurality of needle bars 208 on the top strip 201 can be through two lift post 205 vertical sliding, adjust the height of a plurality of needle bars 208 on the top strip 201, make a plurality of needle bars 208 insert the tip of animal tissue, make things convenient for two top strips 201 to stretch the animal tissue.

Referring to fig. 1-10, an embodiment of the present invention is provided, which solves the main technical problem of pressing a plurality of needle bars 208 into animal tissue, and further,

because the animal tissue processing device further comprises a baffle plate 207, the lower end of each lifting column 205 is provided with the baffle plate 207, each lifting column 205 is sleeved with a compression spring, and the compression spring provides downward force for the plurality of needle rods 208 on the lifting columns 205 and the top strip 201, so that the plurality of needle rods 208 are pressed into animal tissue.

Referring to fig. 1-10, an embodiment of the present invention is provided, which solves the main technical problem of driving the top bar 201 and the plurality of needle bars 208 to move left and right, and further,

because animal tissue processing apparatus still includes well seat 102, telescopic link I103 and V pole 209, fixedly connected with V pole 209 between the downside of two sliders 206 of top strip 201 downside, the downside middle part of handling board 101 sets up well seat 102, and the both sides of well seat 102 have all set up telescopic link I103, and the expansion end difference fixed connection of two telescopic link I103 is on two V poles 209. And then the telescopic rod I103 can drive the V-shaped rod 209 to move left and right, and further drive the two sliding blocks 206 on the V-shaped rod 209 to move left and right, and finally drive the top strip 201 and the plurality of needle rods 208 to move left and right.

Referring to fig. 1 to 10, an embodiment of the present invention is provided, which solves a main technical problem of fixing an animal tissue by pressing a pressing bar 203 against an upper side of the animal tissue, and further,

because animal tissue processing apparatus still includes screw thread post 202, depression bar 203 and bead 204, the downside of every top strip 201 all is provided with depression bar 203, two screw thread posts 202 of fixedly connected with on the depression bar 203, two screw thread posts 202 are all inserted on top strip 201, the downside that threaded connection has nut depression bar 203 on the screw thread post 202 is provided with bead 204, and then can install depression bar 203 in the below of a plurality of needle bars 208, can press the upside at animal tissue through depression bar 203 at this moment, and then it is fixed with animal tissue, be convenient for cut out the horizontal knife groove on animal tissue through flat sword 501 on next step.

Referring to fig. 1-10, an embodiment of the present invention is provided, which solves the main technical problem of using a flat blade 501 to slide laterally to cut a lateral incision on animal tissue, and further,

because the animal tissue processing device further comprises the flat knife 501, the flat knife 501 is arranged on the right side of the processing plate 101, the flat knife 501 can move up and down, left and right relative to the processing plate 101, and then the flat knife 501 is moved to one side of the animal tissue, and at this time, a transverse knife slot can be cut on the animal tissue by using the transverse sliding of the flat knife 501.

A method for culturing cells by using a cell culture system comprises the following steps:

s1: taking an animal tissue block;

s1: cutting a plurality of vertical knife grooves and a transverse knife groove on an animal tissue block by using an animal tissue processing device;

s1: treating animal tissue with trypsin to disperse the animal tissue into a plurality of single cells;

s1: the animal cells are cultured.

The animal tissue treatment device further comprises a bottom frame 401, a telescopic rod II406, a trapezoidal rail 407, a side rod 408, a motor base 502, a motor II503, a friction wheel 504 and a friction rod 505, the right end of the bottom frame 401 is provided with the side rod 408, the trapezoidal rail 407 is vertically arranged on the side rod 408, the motor base 502 is slidably connected onto the trapezoidal rail 407, the telescopic rod II406 is fixedly connected onto the side rod 408, the movable end of the telescopic rod II406 is fixedly connected onto the motor base 502, the friction rod 505 is transversely slidably connected onto the upper portion of the motor base 502, the flat knife 501 is fixedly connected onto the left end of the friction rod 505, the motor II503 is fixedly connected onto the motor base 502, the friction wheel 504 is fixedly connected onto an output shaft of the motor II503, and the friction wheel 504 and the friction rod 505 are in friction transmission.

Referring to fig. 1-10, an embodiment of the present invention is provided, which solves the main technical problem of driving the flat blade 501 and the friction rod 505 to move vertically and driving the flat blade 501 to move horizontally, and further,

when the telescopic rod II406 is telescopic, the motor base 502 is driven to vertically slide on the trapezoidal rail 407, so that the flat knife 501 and the friction rod 505 are driven to vertically move, the friction wheel 504 is driven to rotate by the motor II503, so that the friction rod 505 can be driven to slide left and right on the motor base 502, and the flat knife 501 is driven to horizontally move.

Animal tissue processing apparatus still includes telescopic link II402, gleitbretter 403, lead screw 404, motor I405 and center pillar 409, gleitbretter 403 lateral sliding connects on underframe 401, fixedly connected with center pillar 409 on the gleitbretter 403, the vertical sliding connection of lifter 301 is on center pillar 409, fixedly connected with telescopic link II402 on the gleitbretter 403, the expansion end fixed connection of telescopic link II402 is on lifter 301, the lower part fixedly connected with motor I405 of side lever 408, fixedly connected with lead screw 404 on motor I405's the output shaft, lead screw 404 passes through screw-thread fit with center pillar 409.

Referring to fig. 1-10, an embodiment of the present invention is provided, which solves the main technical problem of adjusting the positions of two vertical knives 304 to cut a plurality of vertical knife slots on animal tissue, and further,

when the motor I405 rotates, the screw rod 404 is driven to rotate by taking the axis of the screw rod as a shaft, the middle column 409 and the sliding sheet 403 are driven to move left and right, when the telescopic rod II402 stretches, the lifting bar 301 is driven to vertically slide on the middle column 409, the two vertical knives 304 are driven to move up and down and left and right, and a plurality of vertical knife grooves are formed in animal tissues by adjusting the positions of the two vertical knives 304.

The animal tissue processed by the animal tissue processing device comprises an animal tissue main body 601, a transverse knife groove 602 and a vertical knife groove 603, wherein the animal tissue main body 601 is provided with a plurality of vertical knife grooves 603 from left to right, the plurality of vertical knife grooves 603 are arranged in tandem without cutting the animal tissue main body 601, and the animal tissue main body 601 is provided with the transverse knife groove 602 penetrating from left to right.