阅读说明：本技术 带盖样本的前处理方法、前处理装置以及样本分析仪 (Pretreatment method and pretreatment device for sample with cover, and sample analyzer ) 是由 甘裕明 王鑫润 朱星才 于 2020-05-22 设计创作，主要内容包括：本发明提供了一种带盖样本的前处理方法、前处理装置以及样本分析仪,该前处理装置包括：离心机构,对带盖样本进行离心；缓存平台,用于供离心后的带盖样本进行静置；去盖机构,用于对静置完成后的带盖样本进行去盖操作；运输机构,用于将离心后的带盖样本运送至缓存平台,及将静置完成后的带盖样本运送至去盖机构。该静置过程极大地降低了去盖过程中产生气溶胶的可能性,从而提高了周围环境的生物安全性。(The invention provides a pretreatment method, a pretreatment device and a sample analyzer for a sample with a cover, wherein the pretreatment device comprises: a centrifugation mechanism for centrifuging the sample with the cover; the buffer storage platform is used for standing the centrifuged sample with the cover; the cover removing mechanism is used for performing cover removing operation on the covered sample after standing; and the transportation mechanism is used for transporting the centrifuged sample with the cover to the buffer storage platform and transporting the sample with the cover after standing to the cover removing mechanism. The resting process greatly reduces the possibility of aerosol generation during decapping, thereby improving the biological safety of the surrounding environment.)

1. A pretreatment method of a sample with a cover is characterized by comprising the following steps:

centrifuging the capped sample;

after centrifugation is finished, standing the sample with the cover for a preset time;

a decapping module performs a decapping operation on the capped sample.

2. The method for pretreating a capped sample according to claim 1, wherein the standing treatment for the preset time after the centrifugation comprises:

placing the centrifuged capped sample in a specific area such that the capped sample remains stationary for a preset time.

3. The method for pretreating a capped specimen as recited in claim 1, wherein the preset time is a parameter set for each capped specimen.

4. The method for pretreating a capped sample according to claim 1, wherein the preset time is a parameter set for a plurality of capped samples.

5. The method for pretreating a capped sample according to claim 1, wherein the preset time is set according to centrifugation parameters of a centrifugation process.

6. The method for pretreating a capped sample according to claim 5, wherein the centrifugation parameter comprises a rotation speed or a centrifugal force at which the capped sample is centrifuged; the length of the preset time is positively correlated with the rotational speed or the centrifugal force.

7. The method for pretreating a capped sample according to claim 5, wherein the centrifugation parameter comprises a centrifugation time of the capped sample, and the preset time has a positive correlation with the centrifugation time of the capped sample.

8. The method for pretreating a capped sample according to claim 5, wherein the centrifugation parameters comprise a rotation speed and a centrifugation time for centrifuging the capped sample, and the preset time is set based on the centrifugation time and the rotation speed.

9. A pretreatment method of a sample with a cover is characterized by comprising the following steps:

centrifuging the sample with the cover by a centrifugal module;

after the centrifugation is finished, performing intermediate processing on the covered sample for a preset time through a cache platform, wherein the intermediate processing comprises standing processing, a transportation process of transporting the covered sample from the centrifugation module to the cache platform after the centrifugation is finished, and a transportation process of transporting the covered sample from the cache platform to the decapping module after the standing processing is finished;

the decapping module performs a decapping operation on the capped sample.

10. A pretreatment device for a sample with a cover, comprising:

a centrifugation mechanism that centrifuges the sample with the cover;

the buffer storage platform is used for standing the centrifuged sample with the cover;

the cover removing mechanism is used for performing cover removing operation on the sample with the cover after standing;

and the transportation mechanism is used for transporting the centrifuged sample with the cover to the buffer storage platform and transporting the sample with the cover after standing to the cover removing mechanism.

11. The pre-processing apparatus for a capped sample as recited in claim 10, wherein the buffer stage is disposed adjacent to the decapping module relative to the centrifugation module.

12. A sample analyzer comprising the capped sample pretreatment device according to any one of claims 10 to 11, a sample aspirating device for aspirating the capped sample, and an analyzing device for analyzing the sample.

Technical Field

The invention relates to the field of biochemical immunoassay, in particular to a pretreatment method and a pretreatment device for a sample with a cover and a sample analyzer.

Background

In the field of biochemical immunoassays, the detection procedure of a sample is generally as follows: firstly, a sample is extracted from a detection object through a vacuum collection tube, the sample is sent to a centrifugation module for centrifugation, and after the centrifugation of the sample is finished, the sample tube needs to be uncovered and then sent to an analysis instrument for detection. For TLA systems, the decapping module is typically located adjacent to or in close proximity to the centrifuge module, which is typically run in a high speed environment, and the freshly centrifuged sample is extremely susceptible to aerosol generation during decapping. At present, all TLA systems do not control the timing of decapping, after a centrifugal module finishes a centrifugal action, a sample is sent to a decapping module, the decapping module detects that the uncapped sample arrives and then carries out decapping action, the probability of aerosol generation by decapping is quite high, and the generated aerosol has great biological potential safety hazard.

Disclosure of Invention

In view of the above, the present invention provides a pretreatment method, a pretreatment device and a sample analyzer for a sample with a cap, so as to solve the problem that aerosol is easily generated and biological safety hazard is caused when a sample with a cap, which is just centrifuged, is uncapped. The technical scheme is as follows:

a pretreatment method of a sample with a cover is characterized by comprising the following steps: centrifuging the sample with the cover; after the centrifugation is finished, standing the sample with the cover for a preset time; the decapping module performs a decapping operation on the capped sample.

Optionally, after the centrifugation is completed, the standing treatment of the sample with the cover for a preset time includes: placing the centrifuged capped sample in a specific area so that the capped sample remains stationary for a preset time.

Alternatively, the preset time is a parameter set separately for each sample with a cap.

Optionally, the preset time is a parameter uniformly set for a plurality of samples with covers.

Alternatively, the preset time is set according to the centrifugation parameters of the centrifugation process.

Optionally, the centrifugation parameter comprises a rotational speed or a centrifugal force at which the lidded sample is centrifuged; the length of the preset time is in positive correlation with the rotation speed or the centrifugal force.

Optionally, the centrifugation parameter comprises a centrifugation time of the covered sample, and the length of the preset time is in positive correlation with the centrifugation time of the covered sample.

Optionally, the centrifugation parameters include a rotation speed and a centrifugation time when the sample with the cover is centrifuged, and the preset time is set based on the centrifugation time and the rotation speed.

A pretreatment method of a sample with a cover is characterized by comprising the following steps: centrifuging the sample with the cover by a centrifugal module; after the centrifugation is finished, performing intermediate treatment on the capped sample for a preset time through a buffer storage platform, wherein the intermediate treatment comprises standing treatment, a transportation process of transporting the capped sample from the centrifugation module to the buffer storage platform after the centrifugation is finished, and a transportation process of transporting the capped sample from the buffer storage platform to a cap removing module after the standing treatment is finished; the decapping module performs a decapping operation on the capped sample.

A pretreatment device for a sample with a cover, comprising: a centrifugation mechanism for centrifuging the sample with the cover; the buffer storage platform is used for standing the centrifuged sample with the cover; the cover removing mechanism is used for performing cover removing operation on the covered sample after standing; and the transportation mechanism is used for transporting the centrifuged sample with the cover to the buffer storage platform and transporting the sample with the cover after standing to the cover removing mechanism.

Optionally, the caching platform is disposed adjacent to the decapping module relative to the centrifuge module.

A sample analyzer comprises the pretreatment device with the cover sample, a sample sucking device and an analysis device, wherein the sample sucking device is used for sucking the sample after the cover is removed, and the analysis device is used for analyzing the sample.

Compared with the prior art, the invention has the following beneficial effects:

the pretreatment method, the pretreatment device and the sample analyzer for the sample with the cover, provided by the invention, can realize that the sample with the cover is firstly kept stand for the preset time after being centrifuged, and then is centrifuged. The resting process greatly reduces the possibility of aerosol generation during decapping, thereby improving the biological safety of the surrounding environment.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.

FIG. 1 shows a schematic view of a pre-processing apparatus for samples with covers;

FIG. 2 is a schematic view of another pre-processing apparatus for processing samples with lids, the pre-processing apparatus including a controller;

FIG. 3 shows a schematic diagram of a sample analyzer;

FIG. 4 shows a flow diagram of a method of pre-processing a capped sample;

fig. 5 shows an operation interface of a controller of the preprocessing apparatus in fig. 2;

FIG. 6 shows a flow chart of another method of pre-processing a capped sample.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The present invention will be described in further detail with reference to the following detailed description and accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

The pretreatment apparatus 10 is an apparatus for pretreating a sample with a cap, and the pretreatment is a treatment process performed before a specific test or analysis is performed on the sample.

Fig. 1 provides an embodiment of a pretreatment apparatus 10 for a covered sample, the pretreatment apparatus 10 including a centrifuge module 11, a buffer stage 12, a decapping module 13, and a transport mechanism 14. Wherein, the centrifugation module 11 is used for centrifuging the sample with the cover, and the number of the centrifugation modules 11 can be one or more. The decapping module 13 is used for decapping the centrifuged sample — as will be understood, capping, membrane adding, decapping and decapping the sample herein, it means to cap, membrane adding, decapping and decapping the sample tube containing the sample; typically, the sample is uncapped after centrifugation for subsequent dispensing or aspiration by a dispensing module or an analysis module. The buffer platform 12 is used for standing the centrifuged sample with the cover, and the buffer platform 12 at least comprises a buffer box, an accommodating part and other structures which can stand and store the sample with the cover; optionally, the buffer platform 12 may further include a temperature adjusting device capable of performing constant temperature or variable temperature treatment on the covered samples stored therein; optionally, the buffer platform 12 may further include a shifting device for shifting the covered samples stored therein; optionally, the buffer platform 12 may further comprise a fixing device for fixing the covered sample stored therein, so that the covered sample is in a stationary state with respect to the buffer platform 12. The cache platform 12 is disposed between the centrifuge module 11 and the decapping module 13. Preferably, the buffer platform 12 is disposed adjacent to the decapping module 13, so as to reduce the possibility of oscillation during the transportation of the settled capped samples from the buffer platform 12 to the decapping module 13. The transportation mechanism 14 is used for transporting the centrifuged capped samples to the buffer platform 12 and sending the standing capped samples to the decapping mechanism. The centrifuge module 11, the buffer platform 12 and the decapping module 13 are all connected to a transport mechanism 14.

Fig. 2 provides another embodiment of the pre-processing apparatus 10 for the capped samples, wherein the pre-processing apparatus 10 not only comprises the centrifugation module 11, the buffer platform 12, the decapping module 13, and the transportation mechanism 14, but also comprises the controller 15, and the controller 15 is used for reading and processing the centrifugation parameters of the capped samples and controlling the motion trajectory of the capped samples so as to improve the automation degree and the intelligence degree of the whole pre-processing process. The centrifuge module 11 is connected to the controller 15; further, the cache platform 12 or the decapping module 13 is also connected to the controller 15.

Fig. 3 provides an embodiment of a sample analyzer 100, wherein the sample analyzer 100 comprises a pre-processing device 10 for a sample with a cover, a sample sucking device 20 and an analyzing device 30, the sample sucking device 20 is used for sucking the sample with the cover removed, and the analyzing device 30 is used for analyzing the sample.

Fig. 4 provides a first embodiment of a method for preprocessing a sample with a cover, which can be specifically described in detail with reference to the structural embodiment of the preprocessing apparatus 10 for preprocessing a sample with a cover shown in fig. 2:

in step S11, the sample with the cap is centrifuged;

specifically, after the sample is sorted and identified by the sample input device, the sample is transported to the centrifugal module 11 by the transportation mechanism 14, and the centrifugal module 11 receives the covered sample and performs centrifugal processing on the covered sample. Optionally, after the centrifugation process is completed, the serum detection module may further perform a serum detection on the sample with the cover, that is, detect whether the serum amount of the sample is sufficient and/or the serum quality of the sample is qualified, so as to determine whether the centrifuged sample can be used for the subsequent measurement.

In step S12, after the centrifugation is completed, the sample with the cover is subjected to a standing treatment for a predetermined time;

the capped sample after centrifugation is transported to the buffer storage platform 12 from the centrifugation module 11 through the transportation mechanism 14, the buffer storage platform 12 receives the capped sample and places the capped sample at a corresponding position for standing processing for a preset time, so that the capped sample is in a static state within the preset time, and the capped sample is transported to the uncapping module 13 after standing.

Specifically, after the centrifugation module 11 completes the centrifugation process on the capped sample, the controller 15 reads the time when the centrifugation of the capped sample is completed, i.e., the centrifugation completion time. Since the transportation time for transporting the capped sample from the centrifugation module 11 to the buffer platform 12 is known, after the preset time is set, the controller 15 can calculate the time for completing the standing processing of the capped sample, i.e. the standing ending time, from the centrifugation ending time of the capped sample, and after the current time reaches the standing ending time, the controller 15 sends an instruction, and the transportation mechanism 14 transports the capped sample to the uncapping module 13.

Optionally, the user directly sets the preset time value according to the actual requirement for the setting condition of the preset time. Specifically, the length of the preset time is adjusted, for example, based on how much the environment is to ensure biological safety; or, adjusting the length of the preset time based on the source of the capped specimen itself or its possible biological safety risk; or, the length of the preset time is adjusted based on the degree of urgency of the detection work.

For the setting condition of the preset time, optionally, the preset time is set according to the centrifugation parameter. The centrifugation parameters are related to the centrifugation of the sample with the cover. The centrifugation parameter may include a rotation speed or a centrifugal force of the capped sample during centrifugation of the capped sample, and the length of the preset time is in positive correlation with the rotation speed or the centrifugal force, that is, the larger the rotation speed of the capped sample during centrifugation is, the longer the preset time is, or the larger the centrifugal force of the capped sample during centrifugation is, the longer the preset time is. The centrifugation parameters can also comprise centrifugation time, namely the time length consumed by the sample with the cover to finish the centrifugation process, and the length of the preset time is in positive correlation with the centrifugation time of the sample with the cover, namely the longer the time used by the sample with the cover in the centrifugation process is, the longer the preset time is; the centrifugation parameters may also include two parameters, i.e., a rotation speed and a centrifugation time for centrifuging the sample with the cover, and the preset time is set based on the centrifugation time and the rotation speed, for example, the length of the preset time and the centrifugation time and the rotation speed satisfy a certain corresponding relationship or functional relationship, and the rotation speed and the centrifugation time need to be considered comprehensively when the preset time is set.

Fig. 5 provides an operation interface of the controller 15 under which the user completes setting of the preset time. When the preset time needs to be directly set, selecting a setting mode of 'setting the preset time', and selecting a specific value of the preset time; when the required preset time is set according to the centrifugal parameters of the centrifugal processing, selecting 'preset time setting based on the centrifugal parameters', and after the centrifugal parameters and the parameter values are set, selecting the specific value of the preset time. For example, a user wants to set a 10min resting time for a centrifuged sample, first selects the option of "preset time setting" and then selects "10 min" in its corresponding time menu. For another example, a user wants to set a preset time according to a centrifugal parameter, take a centrifugal force as an example of the centrifugal parameter to be used as a basis, first select an option of "preset time setting based on the centrifugal parameter", then set a plurality of numerical ranges of the centrifugal force (e.g., 4 numerical ranges of the centrifugal force shown in the table in fig. 5) in a corresponding subordinate table, and set corresponding preset times for the centrifugal forces in different numerical ranges, as shown in fig. 5, when the centrifugal force is greater than 0 and less than or equal to 10G, the preset time is 5min, and when the centrifugal force is greater than 10G and less than or equal to 20G, the preset time is 10min, and then. After the setting is completed, the samples with the cover, the centrifugal force of which satisfies different conditions, are subjected to the standing treatment for the preset time under the control of the controller 15. Similarly, when the centrifugal parameter is the rotating speed or the centrifugal time, the same is set. When the centrifugation parameters include two parameters (rotation speed and centrifugation time, or centrifugal force and centrifugation time), the two parameters need to be set for conditions, optionally, a plurality of numerical value ranges can be respectively set for the two centrifugation parameters, and when the centrifugation parameters of the sample simultaneously satisfy a certain numerical value range of the two parameters, the standing processing of the corresponding preset time under the condition is executed.

It should be noted that, at this time, the user may select the centrifugation parameters to be referred to, and when a certain centrifugation parameter is not selected, the centrifugation parameter is not valid. For example, the values of the preset time and the parameter values of the centrifugation parameter shown in the interface in fig. 5 are only used as an example, and the user can select and set the values according to the requirement.

In addition, regarding the validation object of the preset time, the preset time may be a parameter set for one sample with a cover, or may be a parameter set for a plurality of samples with a cover in a unified manner, that is, when the preset time is set, a user may select that the setting is only validated for one sample with a cover or validated for a plurality of samples with a cover, so as to ensure flexibility of actual operation and better meet the scene requirements of the user.

As for the effective time of the preset time, as one implementation mode, the effective time takes effect in real time after the preset time is set, and after the preset time is set by a user, one or more samples subjected to pretreatment follow the setting; as another embodiment, on the premise that there is currently already a preset time setting, the user may individually set the preset time for one or more specific samples to be preprocessed in the future, perform the setting on the specific sample when it is preprocessed, and still perform the current setting on other samples.

Step S13, the decapping module 13 performs decapping operation on the taped sample;

the capped samples after the completion of the standing are transported from the buffer platform 12 to the uncapping module 13 by the transport mechanism 14, and the uncapping module 13 receives the capped samples and performs an uncapping operation on the capped samples. Optionally, after the decapping operation is completed on the sample, the sample may be divided by the dispensing module, for example, one sample may be divided into a plurality of samples, so as to be subsequently sent to different analysis devices for measurement.

Fig. 6 provides a second embodiment of the method for preprocessing the sample with the cover, which can be specifically described in detail with reference to the structural embodiment of the preprocessing device 10 for preprocessing the sample with the cover shown in fig. 2:

step S21, the centrifugation module 11 performs centrifugation on the sample with the cover;

specifically, after the sample is sorted and identified by the sample input device, the sample is transported to the centrifugal module 11 by the transportation mechanism 14, and the centrifugal module 11 receives the covered sample and performs centrifugal processing on the covered sample.

Step S22, after the centrifugation is completed, performing intermediate processing on the capped sample by the buffer platform 12 for a preset time, where the intermediate processing includes standing processing, a transportation process of transporting the capped sample from the centrifugation module 11 to the buffer platform 12 after the centrifugation is completed, and a transportation process of transporting the capped sample from the buffer platform 12 to the decapping module 13 after the standing processing is completed.

Step S23, the decapping module 13 performs decapping operation on the taped sample;

optionally, after the decapping operation is completed on the sample, the sample may be divided by the dispensing module, for example, one sample may be divided into a plurality of samples, so as to be subsequently sent to different analysis devices for measurement.

The difference between the pre-processing method of the present embodiment and the pre-processing method of the covered sample of the first embodiment is that the processing procedure included in the preset time in step S21 is different, that is, the preset time is defined differently, the preset time in the first embodiment only includes the time for performing the standing processing on the covered sample, and in this embodiment, the preset time includes not only the standing time for performing the standing processing on the covered sample in the buffer platform 12, but also the transportation time for transporting the covered sample from the centrifugation platform 11 to the buffer platform 12 after the centrifugation is completed and the transportation time for transporting the covered sample from the buffer platform 12 to the uncapping module 13 after the standing processing is completed.

Specifically, after the centrifugation module 11 completes the centrifugation process on the capped sample, the controller 15 reads the time at which the centrifugation of the capped sample is completed, i.e., the centrifugation completion time. Since the transportation time for transporting the capped sample from the centrifugation module 11 to the decapping module 13 is known, after the preset time is set, the controller 15 may push out the time required for the standing process, i.e., the standing time, and further, after the time for completing the centrifugation S of the capped sample reaches the standing completion time, the controller 15 issues an instruction to transport the capped sample from the buffer platform 12 to the decapping module 13. Alternatively, after the centrifugation module 11 completes the centrifugation process on the sample with the cover, the controller 15 reads the time when the centrifugation of the sample with the cover is completed, i.e. the centrifugation completion time, and the time when the sample with the cover reaches the decapping module 13, i.e. the arrival time, can be calculated from the centrifugation completion time and the preset time. Since the transit time for transporting the capped sample from the buffer platform 12 to the decapping module 13 is known, the controller 15 can calculate the end time of standing, and after the current time reaches the end time of standing, the controller 15 issues an instruction to transport the capped sample from the buffer platform 12 to the decapping module 13. In short, the operation method of the controller 15 is not limited herein, as long as the capped sample can be transported from the buffer platform 12 to the decapping module 13 after the standing is completed according to the preset time.

In the present embodiment, the preset time is defined to include the standing time of the sample and the whole transportation time from the centrifuge module to the decapping module, and the setting conditions of the preset time, the operation interface of the controller 15, the effective object of the preset time, and the effective time are the same as those in the first embodiment, and may be applied to this embodiment, which will not be described herein.

Those skilled in the art will appreciate that all or part of the functions of the various methods in the above embodiments may be implemented by hardware, or may be implemented by computer programs. When all or part of the functions of the above embodiments are implemented by a computer program, the program may be stored in a computer-readable storage medium, and the storage medium may include: a read only memory, a random access memory, a magnetic disk, an optical disk, a hard disk, etc., and the program is executed by a computer to realize the above functions. For example, the program may be stored in a memory of the device, and when the program in the memory is executed by the processor, all or part of the functions described above may be implemented. In addition, when all or part of the functions in the above embodiments are implemented by a computer program, the program may be stored in a storage medium such as a server, another computer, a magnetic disk, an optical disk, a flash disk, or a removable hard disk, and may be downloaded or copied to a memory of a local device, or may be version-updated in a system of the local device, and when the program in the memory is executed by a processor, all or part of the functions in the above embodiments may be implemented.

The present invention has been described in terms of specific examples, which are provided to aid understanding of the invention and are not intended to be limiting. Variations of the above-described embodiments may be made by those skilled in the art, consistent with the principles of the invention.