阅读说明：本技术 一种灵活的光学微手系统及粒子操控方法 (Flexible optical micro-hand system and particle control method ) 是由 苑立波 杨世泰 于 2021-08-26 设计创作，主要内容包括：本发明提供的是一种灵活的光学微手系统及粒子操控方法,其特征是：所述的光学微手系统包括十通道动力光源、光纤耦合器、第一光纤衰减器、第二光纤衰减器、光纤相位调制器、三芯光纤扇入扇出器、三芯光纤、九芯光纤扇入扇出器、九芯光纤、显微镜、计算机。九芯光纤具有3×3的方形分布的纤芯,其纤端有锥体圆台结构,可反射聚焦八个周边纤芯内的操纵光束,九芯光纤的中间芯为少模纤芯。可以通过控制这些纤芯内传输的操控光的强度、相位来实现对微粒的捕获、旋转等姿态调整功能,还可以实现微粒的定向运输功能。可广泛用于复杂的微粒光操纵领域。(The invention provides a flexible optical micro-hand system and a particle control method, which are characterized in that: the optical micro-hand system comprises a ten-channel power light source, an optical fiber coupler, a first optical fiber attenuator, a second optical fiber attenuator, an optical fiber phase modulator, a three-core optical fiber fan-in fan-out device, a three-core optical fiber, a nine-core optical fiber fan-in fan-out device, a nine-core optical fiber, a microscope and a computer. The nine-core optical fiber has a 3 x 3 square distributed fiber core, the fiber end of the nine-core optical fiber is provided with a cone frustum structure, the control light beams in eight peripheral fiber cores can be reflected and focused, and the middle core of the nine-core optical fiber is a few-mode fiber core. The gesture adjusting functions of capturing, rotating and the like of the particles can be realized by controlling the intensity and the phase of the control light transmitted in the fiber cores, and the directional transportation function of the particles can also be realized. Can be widely used in the field of complicated particle light manipulation.)

1. A flexible optical micro-hand system, characterized by: the optical micro-hand system comprises a ten-channel power light source, an optical fiber coupler, a first optical fiber attenuator, a second optical fiber attenuator, an optical fiber phase modulator, a three-core optical fiber fan-in fan-out device, a three-core optical fiber, a nine-core optical fiber fan-in fan-out device, a nine-core optical fiber, a microscope and a computer; nine fiber cores of the nine-core optical fiber are distributed in a 3 multiplied by 3 square grid mode, one middle core is a three-mode fiber core, and eight peripheral cores are single-mode fiber cores; (2) the output fiber end of the nine-core optical fiber is provided with a cone frustum structure; (3) the first to eighth output channels of the ten-channel power light source are respectively connected with eight peripheral core input optical fibers of the nine-core optical fiber fan-in fan-out device, and control light beams transmitted in the eight peripheral optical fibers of the input nine-core optical fiber can be reflected and focused by the fiber end cone frustum; (4) the ninth channel of the ten-channel power light source is connected with the optical fiber coupler and then divided into two paths with equal power, wherein one path is sequentially connected with the first optical fiber attenuator and the optical fiber phase modulator and then input into the first channel of the three-core optical fiber fan-in fanout device, and the other path is connected with the second optical fiber attenuator and then input into the second channel of the three-core optical fiber fan-in fanout device; (5) the tenth channel of the ten-channel power light source is connected with the third channel of the three-core optical fiber fan-in fan-out device; (6) the three-core optical fiber and the nine-core optical fiber fan-in fanout device are connected through a middle core channel, and a first channel and a second channel of the three-core optical fiber fan-in fanout device respectively and correspondingly excite two LP (Low-level) fiber cores of a middle three-mode fiber core of the nine-core optical fiber₁₁Modes, by adjusting the first and second fiber attenuators and the fiber phase modulator, can be combined to produce a three-mode fiberThe first-order eddy optical rotation in the core, the three-core optical fiber fanning-in third channel of the fanout device can excite LP of a three-mode fiber core in the middle of the nine-core optical fiber₀₁Mode(s).

2. The flexible optical micro hand system according to claim 1, wherein: the fiber core of the nine-core optical fiber is externally provided with a low-refractive-index isolation layer doped with fluorine.

3. The flexible optical micro hand system according to claim 1, wherein: the three-core optical fiber comprises a fluorine-doped low-refractive-index cladding layer on the outermost layer, a pure quartz cladding layer and three single-mode fiber cores which are distributed in a triangular mode and have different effective refractive indexes of mode fields.

4. The flexible optical micro hand system according to claim 1, wherein: the nine-core optical fiber fan-in fan-out device is formed by inserting eight double-clad optical fibers and three-core optical fibers into a fluorine-doped nine-hole sleeve, thermally insulating, tapering, cutting and welding the nine-core optical fibers; the eight double-clad optical fibers are correspondingly connected with the peripheral fiber cores of the nine-core optical fiber after tapering, and the three-core optical fiber is correspondingly connected with the middle core of the nine-core optical fiber after tapering, so that the mode division multiplexer of the middle core of the nine-core optical fiber is formed.

5. The flexible optical micro hand system according to claim 1, wherein: the inclined plane of the cone frustum at the fiber end of the nine-core optical fiber can be plated with a layer of reflective metal film.

6. The flexible optical micro hand system according to claim 1, wherein: the nine-core fiber end is provided with a fiber Bragg grating, and a matched fiber grating demodulator is arranged in the system and used for sensing the temperature of the fiber end.

7. The flexible optical micro hand system according to claim 1, wherein: the particle posture adjusting and controlling method of the optical micro-hand system comprises the following steps:

1) for the nine-core lightNine cores of the fiber are numbered as C_ijWherein i and j are respectively a row number and a column number, and nine fiber cores can be divided into 2 groups according to functional classification, wherein the nine fiber cores comprise a capturing fiber core group and a rotating fiber core group;

2) wherein the catching fiber core group comprises C₁₁、C₁₃、C₃₁And C₃₃The power light beams in the fiber cores of the group are focused in front of the fiber end after being reflected by the cone frustum at the fiber end to form three-dimensional optical tweezers which can stably capture particles;

3) spin core groups can be divided into xoy, yoz and xoz-plane spin with core C₂₁And C₂₃The power of the power beams in the two fiber cores can control the clockwise and anticlockwise rotation of the particles on the xoz plane; core C₁₂And C₃₂The power of the power beams in the two fiber cores can control the clockwise and anticlockwise rotation of the particles on the yoz surface; core C₂₂For few-mode core, C can be controlled by inputting the first and second channels of the fan-out device₂₂The core vortex optically rotates in the left-handed and right-handed directions and the intensity, and vortex light acts on the captured particles to enable the captured particles to rotate clockwise or anticlockwise in the xoy plane;

4) the three-dimensional capture and three-dimensional rotation operation of the particles can be realized through the steps, and the arbitrary posture adjustment of the particles can be realized under a microscope.

8. The flexible optical micro hand system according to claim 1, wherein: the control method for directionally throwing out the particle postures of the optical micro-hand system comprises the following steps:

1) numbering nine cores of the nine-core optical fiber, and marking as C_ijWherein i and j are a row number and a column number, respectively;

2) wherein C is₁₁、C₁₃、C₃₁And C₃₃The power light beam in the fiber core is reflected by the cone frustum at the fiber end and then is focused in front of the fiber end to form three-dimensionalThe optical tweezers can stably capture particles;

3) core C₂₂For few-mode core, C can be controlled by inputting the first and second channels of the fan-out device₂₂The core vortex light has the left-handed and right-handed directions and intensity, and the vortex light acts on the captured particles to position and axially rotate the captured particles in the xoy plane;

4) when the particles are controlled to rotate in a fixed axis mode through the first channel and the second channel of the three-core optical fiber fan-in fan-out device, power beams can be injected into the third channel of the three-core optical fiber fan-in fan-out device, captured particles are ejected in a rotating mode, and the function of directional throwing is achieved.

Technical Field

The invention relates to a flexible optical micro-hand system and a particle control method, belonging to the technical field of micro-manipulation of optical tweezers.

Background

The optical tweezers technology is a technology for forming a trapping potential well on a particle by using a single beam or a multi-beam combined optical field so as to trap and operate the particle. Since the "optical tweezers" technology proposed a single-beam laser-based three-dimensional optical potential well in "observer of edge-beam gradient for electronic devices" in 1986 for achieving three-dimensional spatial control of particles, the technology of "optical tweezers" has been known and developed in a long time. The invention of the optical tweezers enables people to obtain a tool for manipulating tiny particles, which promotes the rapid development of many interdisciplines, and particularly in the field of life sciences, the optical tweezers show incomparable advantages by the characteristics of non-contact and nondestructive detection.

The optical tweezers not only can realize the stable capture of particles, but also has the characteristic of attracting people and can carry out various dynamic operations on tiny particles. The holographic optical tweezers system can generate a desired optical field at will, easily realize the operation of arranging and controlling the movement of a plurality of particles, but has larger space volume, and the operation flexibility of the space dimension is obviously inferior to that of bendable optical tweezers. As for the fiber optical tweezers, the operation function of the fiber optical tweezers on particles is relatively single. The invention patent with the publication number of CN101907743A provides throughput type optical fiber tweezers, which can realize ejection throughput dynamic operation of particles; the invention patent publication CN102222533A proposes a power drill based on a multi-core optical fiber, which can perform a rotation operation on particles. Yuan et al propose a four-core fiber optical tweezers-based particle oscillation device (IEEE Photonics Technology Letters,2016,28(4): 461-. These existing fiber optical tweezers have a relatively single particle manipulation technique.

In order to realize posture adjustment of captured particles, have functions similar to a hand and realize functions of grabbing, three-dimensional rotating, directional throwing and the like of target particles, patent CN201711184344.2 proposes a programmable optical fiber micro-optical hand, and the invention realizes manipulation of capturing, rotating, vibrating, ejecting and the like of particles by programming control of optical power in each fiber core of a 7-core optical fiber optical tweezers. But the steering beam is focused on one point, the moment of the rotating operation is small, the rotating dimension is not very comprehensive, and the control is relatively difficult.

Disclosure of Invention

The invention aims to provide a flexible optical micro-hand system and a method for controlling particles by the system.

The purpose of the invention is realized as follows:

a flexible optical micro-hand system is shown in figure 1, and comprises a ten-channel power light source 1, a fiber coupler 6 and a first optical fiber attenuationThe optical fiber phase modulator comprises a subtractor 7-1, a second optical fiber attenuator 7-2, an optical fiber phase modulator 8, a three-core optical fiber fan-in fan-out device 3, a three-core optical fiber 4, a nine-core optical fiber fan-in fan-out device 5, a nine-core optical fiber 2, a microscope 9 and a computer 10. Wherein 1) nine fiber cores of the nine-core optical fiber 2 are distributed in a 3 multiplied by 3 square grid, the middle core is a three-mode fiber core, and eight peripheral cores are single-mode fiber cores; 2) the output fiber end of the nine-core optical fiber 2 is provided with a cone frustum structure; 3) the first to eighth output channels 1-1 to 1-8 of the ten-channel power light source 1 are respectively connected with eight peripheral core input fibers of the nine-core fiber fanout device 5, and control beams transmitted in the eight peripheral cores of the input nine-core fiber 2 can be reflected and focused by the fiber end cone frustum; 4) the ninth channel 1-9 of the ten-channel power light source 1 is connected with the optical fiber coupler 6 and then divided into two paths with equal power, wherein one path is connected with the first optical fiber attenuator 7-1 and the optical fiber phase modulator 8 in sequence and then input into the first channel of the three-core optical fiber fan-in fanout device 3, and the other path is connected with the second optical fiber attenuator 7-2 and then input into the second channel of the three-core optical fiber fan-in fanout device 3; 5) the tenth channel 1-10 of the ten-channel power light source 1 is connected with the third channel of the three-core optical fiber fan-in fan-out device 3; 6) the three-core optical fiber 4 is connected with the middle core channel of the nine-core optical fiber fan-in fanout device 5, and the first channel and the second channel of the three-core optical fiber fan-in fanout device 3 respectively and correspondingly excite two LPs of the middle three-mode fiber core of the nine-core optical fiber 2₁₁The first-order eddy optical rotation in the three-mode fiber cores can be generated in a combined mode through the adjustment of the first and second optical fiber attenuators 7-1 and 7-2 and the optical fiber phase modulator 8, and the third channel of the three-core optical fiber fan-out device 3 can excite the LP of the middle three-mode fiber core of the nine-core optical fiber 2₀₁Mode(s).

Fig. 2 is a schematic end view of a nine-core optical fiber including nine cores. The eight fiber cores at the periphery are in single mode in a dynamic light wave band, for example, the dynamic light is 980nm or 1064 nm. The middle core supports three modes in dynamic optical band, respectively LP₀₁And two orthogonal LPs₁₁Modes, such as dynamic light at 980nm or 1064 nm; the intermediate core is single-mode in the sensing waveband of the fiber Bragg grating, for example, the sensing waveband is C waveband.

As shown in fig. 3, the core of the nine-core optical fiber is externally provided with a low refractive index isolation layer doped with fluorine for reducing the energy crosstalk between the cores.

The inclined plane of the cone frustum at the fiber end of the nine-core optical fiber 2 can be plated with a layer of reflective metal film.

The nine-core fiber end is provided with a fiber Bragg grating, and a matched fiber grating demodulator is arranged in the system and used for sensing the temperature of the fiber end.

As shown in fig. 4, the three-core optical fiber 4 includes an outermost fluorine-doped low-refractive-index cladding, a pure silica cladding, and three single-mode cores (4-1) - (4-3) with different triangular mode field effective refractive indexes, and is to be used for preparing a mode division multiplexer for a nine-core optical fiber intermediate core.

As shown in fig. 5, the three-core fiber fan-in fan-out device 3 is formed by inserting three double-clad fibers 11 into a three-hole quartz sleeve 12, thermally tapering, cutting, and welding with three-core fibers 4, wherein the other end of the double-clad fibers 11 can be welded with a standard single-mode fiber 13. The end face structure and refractive index profile of the double-clad optical fiber 11 is shown in FIG. 6, and includes a single-mode core 11-1, an inner cladding 11-2, and an outer cladding 11-3. The end face structure of the three-hole quartz sleeve 12 is shown in fig. 7.

Fig. 8 is a diagram showing a change in the structure of the double-clad optical fiber 11 after tapering, the left diagram showing the end surface structure, refractive index distribution, and mode field 14-1 distribution of the double-clad optical fiber 11 before tapering, and the right diagram showing the end surface structure, refractive index distribution, and mode field 14-2 distribution after tapering. It can be seen that before and after tapering, the mode field 14-1 of the optical wave gradually transits from the fiber core 11-1 to the inner cladding 11-2 to form a new mode field 14-2, so that the three-core fiber fan-in fanout 3 can be prepared.

As shown in fig. 9, the nine-core fiber fan-in fan-out device 5 is formed by inserting eight double-clad fibers 11 and three-core fibers 4 into a fluorine-doped nine-hole sleeve 15, thermally insulating, tapering, cutting, and welding with nine-core fibers 2; the eight double-clad optical fibers 11 are correspondingly connected with eight peripheral fiber cores of the nine-core optical fiber 2 after tapering, and the three-core optical fiber 4 is correspondingly connected with the middle core of the nine-core optical fiber 2 after tapering, so that the mode division multiplexer of the middle core of the nine-core optical fiber 2 is formed.

The end view of the fluorine-doped nine-hole ferrule 15 is shown in fig. 10, the hole distribution of the ferrule is consistent with the core distribution of the nine-core optical fiber 2, and the double-clad optical fiber 11 and the three-core optical fiber 4 can be inserted into the hole, wherein the principle of the peripheral eight-core fan-in fan-out is the same as that of the three-core optical fiber fan-in fanout device described above. The mode division multiplexing principle of the middle core adopts the mechanism of a photon lantern.

The vortex light generation mechanism of the nine-core fiber intermediate core is further described below.

As shown in FIG. 11, the middle core of the nine-core optical fiber 2 supports three modes of the dynamic optical band, and (a) is LP₀₁The basic model, (b) and (c) are respectively LP₁₁And (5) molding. According to the mode division multiplexing principle of the photon lantern, three fiber cores (4-1) - (4-3) of the three-core optical fiber 4 are respectively in one-to-one correspondence with three modes, as shown in fig. 12-14.

As shown in FIG. 15, two of the three-core fibers 4 simultaneously correspond to LP₁₁Light is injected into the fiber core of the mode, the phase difference and the power ratio of the two paths of light waves are adjusted through the optical fiber attenuators 7-1 and 7-2 and the optical fiber phase modulator 8 to form a first-order vortex light beam in a combined mode, and the chirality of the vortex light beam can be adjusted.

As shown in fig. 16, the method for adjusting and controlling the particle pose of the optical micro-hand system comprises:

1) the nine cores of the nine-core optical fiber 2 are numbered as C_ijWherein i and j are respectively a row number and a column number, and nine fiber cores can be divided into 2 groups according to functional classification, wherein the nine fiber cores comprise a capturing fiber core group and a rotating fiber core group;

2) wherein the catching fiber core group comprises C₁₁、C₁₃、C₃₁And C₃₃The power light beams in the fiber cores of the group are focused in front of the fiber end after being reflected by the cone frustum 16 of the fiber end to form three-dimensional optical tweezers which can stably capture particles 17;

3) spin core groups can be divided into xoy, yoz and xoz-plane spin with core C₂₁And C₂₃The power beam transmitted inside can act on the captured particles 17 after being reflected by the fiber end cone frustum 16, as shown in fig. 17, the clockwise and counterclockwise rotation of the particles 17 in the xoz plane can be controlled by controlling the power of the power beams in the two fiber cores; core C₁₂And C₃₂Internal transmission power beamThe particles can act on the captured particles 17 after being reflected by the fiber end cone frustum 16, and the clockwise and anticlockwise rotation of the particles on the yoz surface can be controlled by controlling the power of the power beams in the two fiber cores; FIG. 18, core C₂₂For few-mode core, C can be controlled by inputting the first and second channels of the three-core fiber fan-in fan-out device 3₂₂The left-handed and right-handed directions and intensity of core vortex optical rotation, and vortex light acts on the captured particles 17 to enable the captured particles 17 to rotate clockwise or anticlockwise in the xoy plane;

4) the above steps can realize three-dimensional capture and three-dimensional rotation operation of the particles, that is, arbitrary posture adjustment of the particles 17 under the microscope 9.

The control method for directionally throwing out the particle postures of the optical micro-hand system comprises the following steps:

1) numbering nine cores of the nine-core optical fiber, and marking as C_ijWherein i and j are a row number and a column number, respectively;

2) wherein C is₁₁、C₁₃、C₃₁And C₃₃After being reflected by the cone frustum 16 at the fiber end, the power beam in the fiber core is focused in front of the fiber end to form three-dimensional optical tweezers which can stably capture particles 17;

3) core C₂₂For few-mode core, C can be controlled by inputting the first and second channels of the three-core fiber fan-in fan-out 3₂₂The left-handed and right-handed directions and intensity of core vortex optical rotation, vortex light acts on the captured particles 17, so that the captured particles are positioned in the xoy plane and rotate in a fixed axis manner;

4) when the fixed axis rotation of the particles 17 is controlled through the first channel and the second channel of the three-core optical fiber fan-in fanout device 3, power beams can be injected into the third channel of the three-core optical fiber fan-in fanout device 3, the captured particles 17 are ejected in a rotating mode, and the function of directional ejection is achieved.

Compared with the prior art, the invention has the following characteristics:

the special nine-core optical fiber is adopted, the focusing of 4 fiber core transmission light beams on the periphery of the special nine-core optical fiber is utilized, the stable capture of particles is realized, the light beams transmitted in the other four fiber cores can rotate the captured particles clockwise or anticlockwise in two orthogonal planes, the photoinduced rotation function of the middle core vortex light is combined, the fixed axis rotation in the third orthogonal plane of the captured particles is realized, and the three-dimensional posture adjustment of the captured particles is really realized. And then the Gaussian beam with proper intensity is used for realizing the directional ejection of the particles. The rotary directional ejection can realize longer range and higher transmission precision.

Drawings

Fig. 1 is a diagram of an optical micro hand system.

Fig. 2 is a view showing an end face structure of the nine-core optical fiber 2.

FIG. 3 is a nine-core optical fiber having a ring of low index spacer layers surrounding the core.

Fig. 4 is an end face structural view of the three-core optical fiber 4.

Fig. 5 is a block diagram of the three-core fiber fan-in fanout 3.

Fig. 6 is a view showing the end face structure and refractive index distribution of double-clad optical fiber 11.

Fig. 7 is an end face structural view of the three-hole quartz sleeve 12.

FIG. 8 is a schematic diagram of the mode field adiabatic transition from the core to the inner cladding of double-clad fiber 11 during tapering.

Fig. 9 is a block diagram of a nine-core fiber fan-in fanout 5.

Fig. 10 is an end face structural view of the nine-hole low-refractive-index sleeve 15.

FIG. 11 shows the mode field distributions of the three modes supported by the central core of the nine-core optical fiber 2, where (a) is LP₀₁The basic mode, (b) and (c) are LP₁₁And (5) molding.

Fig. 12 to 15 show the input of the different cores of the middle three-core fiber 4 of the nine-core fiber fan-in fanout 5 corresponding to the different output modes of the middle core of the nine-core fiber 2.

Fig. 16 is a structural view of a fiber end taper circular truncated cone 16 of the nine-core optical fiber 2, in which (a) is a three-dimensional structural view, (b) is an end face structural view, and (c) and (d) are respectively cross-sectional axial views in the horizontal and 45-degree directions in (b).

FIG. 17 is a schematic illustration of the rotational operation of the system for trapping particles in the xoz or yoz plane, with (a) (b) indicating clockwise and counterclockwise rotation, respectively, and with core numbering denoting core 45 degreesProjected position on the cross-section, e.g. C₂₁' denotes a core C₂₁Projected at the position of the 45 deg. section.

Figure 18 is a schematic view of the rotational operation of the system for trapping particles in the xoy plane, with (a) (b) indicating clockwise and counterclockwise rotation, respectively.

Fig. 19 is a diagram of an optical micro-hand system with fiber tip temperature monitoring.

Fig. 20 is a schematic diagram of a nine-core optical fiber 2 having a bragg fiber grating 20 at its fiber end for temperature monitoring.

Detailed Description

The invention is further illustrated below with reference to specific examples.

Example (b): optical micro-hands are used for cell capture and pose adjustment.

As shown in fig. 19, a fiber grating demodulation module 18 is added in the optical micro-hand system for monitoring the ambient temperature at the fiber end. The whole system and the connection relation are as follows:

the ten-channel power light source 1 selects an LD pump laser with the wavelength of 980nm, and the output power of each channel is adjustable at 0-100 mW. The absorption coefficient of the wavelength in the water environment is small, and the cell 17 can be effectively prevented from being damaged by local heating of laser. The channels 1-1 to 1-8 of the ten-channel power light source 1 are connected with a nine-core optical fiber fan-in fan-out device 5 and correspondingly communicated with eight peripheral optical cores of the nine-core optical fiber 2. The light wave output by the ninth channel 1-9 of the ten-channel power light source 1 is divided into two paths with equal power through a 3dB optical fiber coupler 6, wherein one path is connected with a first optical fiber attenuator 7-1 and an optical fiber phase modulator 8 and then is connected into the first channel of the three-core optical fiber fan-in fanout device 3, and the other path is connected with a second optical fiber attenuator 7-2 and then is connected into the second channel of the three-core optical fiber fan-in fanout device 3. The light waves output by the tenth channels 1-10 of the ten-channel power light source 1 and the output of the C-band fiber bragg grating demodulation module 18 are combined by a fiber wavelength division multiplexer 19 and then are connected into the third channel of the three-core fiber fan-in fan-out device 3. The first and second channels of the three-core fiber fan-out 3 correspond to the LP of the middle core of the nine-core fiber 2₁₁The power and the phase difference of the two input channels can be adjusted by adjusting the first and the second optical fiber attenuators 7-1 and 7-2 and the optical fiber phase modulator 8Vortex light of the first order is modulated in the middle core of the nine-core optical fiber 2. The end of the nine-core optical fiber is provided with a cone round table 16 structure, and the whole cell manipulation process is carried out under an inverted biological microscope 9, so that the visual operation is realized. The microscope 9, the ten-channel power light source 1, the fiber bragg grating demodulation module 18 and the computer 10 are connected to realize the control of the whole system.

The operation process of the system for single cell posture adjustment comprises the following steps:

(1) nine cores of the nine-core optical fiber 2 are numbered as C_ijWherein i and j are respectively a row number and a column number, and the nine fiber cores 2 can be divided into two groups according to the functional classification, including a catching fiber core group and a rotating fiber core group.

(2) Wherein the catching fiber core group comprises C₁₁、C₁₃、C₃₁And C₃₃And (3) opening a fiber core, and outputting the power of the ten-channel power light source to the channels corresponding to the fiber core group, wherein the power is adjusted to be 20 mW. The power beams in the fiber cores of the group are reflected by the fiber end cone round table 16 and then focused in front of the fiber end to form three-dimensional optical tweezers, and cells 17 are stably captured under the microscope 9.

(3) Meanwhile, the fiber bragg grating demodulation module 18 is turned on, the ambient temperature at the tail end is monitored through the reflection spectrum of the fiber bragg grating 20 at the tail end, and when the ambient temperature exceeds 40 ℃, the system alarms and reduces the output power of the captured light beam.

(4) Spin core groups can be divided into xoy, yoz and xoz-plane spin with core C₂₁And C₂₃The power beam transmitted inside can act on the captured cell 17 after being reflected by the end cone frustum 16, as shown in fig. 17, and the clockwise and counterclockwise rotation of the cell in the xoz plane can be controlled by controlling the power of the power beams in the two fiber cores; core C₁₂And C₃₂The power beams transmitted internally can act on the captured cells 17 after being reflected by the cone frustum of the fiber end, and the clockwise and anticlockwise rotation of the cells on the yoz surface can be controlled by controlling the power of the power beams in the two fiber cores; FIG. 18, core C₂₂For few-mode core, C can be controlled by inputting the first and second channels of the three-core fiber fan-in fan-out device 3₂₂Core vortex optical rotation left-hand and right-hand directions and intensity, vortexThe optical rotation acts on the captured cells 17 to rotate the captured cells 17 clockwise or counterclockwise within the xoy plane.

The three-dimensional capture and three-dimensional rotation operation of the cells can be realized through the steps, and the arbitrary posture adjustment of the cells can be realized under a microscope.

In the description and drawings, there have been disclosed typical embodiments of the invention. The invention is not limited to these exemplary embodiments. Specific terms are used in a generic and descriptive sense only and not for purposes of limitation, the scope of the invention being set forth.