阅读说明：本技术 一种医学检验用基于磁珠技术的空气微生物高效富集装置 (Medical science inspection is with high-efficient enrichment device of air microorganism based on magnetic bead technique ) 是由 马晓 薛娟 刘进伟 于 2021-09-10 设计创作，主要内容包括：一种医学检验用基于磁珠技术的空气微生物高效富集装置,具有箱体、载液器具、振荡器、旋转升降台、磁珠供料器、处理棒组件以及控制单元,处理棒组件具有处理棒本体、超声发生器和电源电路,处理棒设置在振荡器的上方,处理棒本体具有超声波处理部和电磁铁吸附部,超声发生器用于向超声波处理部输送超声能量,电源电路用于驱动电磁铁吸附部。控制单元用于控制振荡器、旋转升降台以及处理棒组件。本发明的富集装置能够实现磁珠提取核酸的全自动操作,将超声分散部件与磁珠的吸附部件结合在一起,即实现了功能的交叉影响,又优化了装置的结构设计。(The utility model provides a medical science inspection is with high-efficient enrichment device of air microorganism based on magnetic bead technique, the box has, the carrier liquid utensil, the oscillator, rotatory elevating platform, the magnetic bead feeder, handle excellent subassembly and the control unit, it has the processing stick body to handle excellent subassembly, ultrasonic generator and power supply circuit, it sets up in the top of oscillator to handle the stick, it has ultrasonic treatment portion and electro-magnet absorption portion to handle excellent body, ultrasonic generator is used for carrying ultrasonic energy to ultrasonic treatment portion, power supply circuit is used for driving electro-magnet absorption portion. The control unit is used for controlling the oscillator, the rotary lifting table and the processing rod assembly. The enrichment device can realize the full-automatic operation of extracting nucleic acid by magnetic beads, and combines the ultrasonic dispersion component and the adsorption component of the magnetic beads, thereby realizing the cross effect of functions and optimizing the structural design of the device.)

1. The utility model provides a medical science inspection is with high-efficient enrichment facility of air microorganism based on magnetic bead technique which characterized in that, it has:

the box body is used for bearing and protecting other parts; and

the liquid carrying appliance is used for carrying treatment liquid and is provided with two liquid carrying chambers which are isolated from each other; and

the oscillator is used for driving the liquid carrying appliance to perform oscillating motion; and

the rotary lifting platform is used for driving the oscillator to lift or rotate; and

a magnetic bead feeder which is loaded with magnetic beads and can quantitatively convey the magnetic beads to the carrier liquid utensil; and

the ultrasonic treatment device comprises a treatment rod assembly, a processing rod and a power circuit, wherein the treatment rod assembly is provided with a treatment rod body, an ultrasonic generator and a power circuit, the treatment rod is arranged above an oscillator, the treatment rod body is provided with an ultrasonic treatment part and an electromagnet adsorption part, the ultrasonic generator is used for conveying ultrasonic energy to the ultrasonic treatment part, and the power circuit is used for driving the electromagnet adsorption part; and

a control unit for controlling the oscillator, the rotary lift table and the processing rod assembly.

2. The enrichment apparatus of claim 1, wherein:

the table top of the oscillator for placing the liquid carrying appliance is provided with a clamping seat, and the liquid carrying appliance can be temporarily fixed on the table top of the oscillator through the clamping seat.

3. The enrichment apparatus of claim 1, wherein:

the rotary lifting platform is provided with a lifting base and a rotary disk arranged on the lifting base.

4. The enrichment apparatus of claim 1, wherein:

the ultrasonic treatment part of the treatment rod body is a stainless steel rod body, one end of the ultrasonic treatment part of the treatment rod body is connected with the ultrasonic generator, and the other end of the ultrasonic treatment part of the treatment rod body is connected with the electromagnet adsorption part.

5. The enrichment apparatus of claim 1, wherein:

the liquid bearing implement has:

the base can be temporarily fixed on the table top of the oscillator through the clamping seat; and

a first treatment canister; and

a second treatment canister;

a first processing section of thick bamboo and second are handled a section of thick bamboo and are all installed on the base through detachable mode.

6. The enrichment apparatus of claim 5, wherein:

the first treatment cylinder and the second treatment cylinder have different appearance colors; or

The wall of the first treatment cylinder and the wall of the second treatment cylinder are provided with different marks.

7. The enrichment apparatus of claim 5, wherein:

and stirring units are arranged at the bottoms of the first treatment cylinder and the second treatment cylinder.

8. The enrichment apparatus of claim 1, wherein:

the magnetic bead feeder includes:

the magnetic bead storage box is used for storing the magnetic beads to be used and is provided with a discharge hole; and

the quantitative feeder is arranged at the discharge port of the magnetic bead storage box.

9. The enrichment apparatus of claim 8, wherein:

the magnetic bead is provided with an iron oxide core, a silane layer wraps the outside of the iron oxide core, and the diameter of the magnetic bead is 20-500 nm.

Technical Field

The invention relates to the technical field of medical auxiliary instruments, in particular to an air microorganism efficient enrichment device based on a magnetic bead technology for medical inspection.

Background

The magnetic bead technology is a molecular biology separation technology, and the magnetic bead technology utilizes the affinity binding of magnetic particles modified on the surface of the magnetic bead technology to biological molecules or cells for separation, can efficiently enrich targets to be separated or detected, and is a convenient, rapid, high-recovery and strong-selectivity method. The magnetic beads are paramagnetic particles with specific shapes, the surface of the magnetic bead is coated with amino, carboxyl terminal or other modified functional groups with the magnetic field strength of about 1000Gs, and macromolecules such as antibodies, antigens, agglutinins, enzymes, nucleic acids and the like can be connected, and the biological activity of the macromolecules can be kept.

Human infectious diseases and animal epidemic diseases always affect human health, troubles the economic development of international travel, trade and livestock breeding industry, and even are utilized as bioterrorism factors to influence social stability. The pathogenic microorganisms exist in the bottom layer of the atmosphere frequently in the form of aerosol, and can bring large-scale threats to human health and animals when reaching a certain concentration, such as SARS.H1N1 influenza, MERS, H7N9 influenza, H5N1 avian influenza, foot-and-mouth disease, African swine fever and the like. Therefore, the method can monitor the microorganisms in the air environment timely, simply and accurately, and has very important significance for guaranteeing the health of people and animals. Nucleic acids are important subjects in modern biological and medical research as carriers of genetic information. In recent years, nucleic acid detection has been rapidly developed in medical tests, and has been widely used in the fields of microbial detection, forensic identification, and the like. Whether the basic research of nucleic acid or the clinical routine nucleic acid detection is carried out, firstly

The nucleic acid is required to be separated and purified. The traditional nucleic acid separation technology comprises the processes of precipitation, high-speed centrifugation and the like, the purification methods have the disadvantages of complicated steps, long time consumption, low recovery rate, harm to health of toxic reagents, environmental pollution and difficulty in realizing automatic operation. The magnetic bead separation technology can well overcome the defects and realize the rapid and efficient preparation of the sample.

Disclosure of Invention

The invention aims to solve the defects of the technical problems and provide an air microorganism high-efficiency enrichment device based on a magnetic bead technology for medical examination.

In order to solve the technical problems, the invention adopts the technical scheme that:

the utility model provides a medical science inspection is with high-efficient enrichment facility of air microorganism based on magnetic bead technique which has:

the box body is used for bearing and protecting other parts; and

the liquid carrying appliance is used for carrying treatment liquid and is provided with two liquid carrying chambers which are isolated from each other; and

the oscillator is used for driving the liquid carrying appliance to perform oscillating motion; and

the rotary lifting platform is used for driving the oscillator to lift or rotate; and

a magnetic bead feeder which is loaded with magnetic beads and can quantitatively convey the magnetic beads to the carrier liquid utensil; and

the ultrasonic treatment device comprises a treatment rod assembly, a processing rod and a power circuit, wherein the treatment rod assembly is provided with a treatment rod body, an ultrasonic generator and a power circuit, the treatment rod is arranged above an oscillator, the treatment rod body is provided with an ultrasonic treatment part and an electromagnet adsorption part, the ultrasonic generator is used for conveying ultrasonic energy to the ultrasonic treatment part, and the power circuit is used for driving the electromagnet adsorption part; and

a control unit for controlling the oscillator, the rotary lift table and the processing rod assembly.

As a further optimization of the above enrichment device:

the table top of the oscillator for placing the liquid carrying appliance is provided with a clamping seat, and the liquid carrying appliance can be temporarily fixed on the table top of the oscillator through the clamping seat.

As a further optimization of the above enrichment device:

the rotary lifting platform is provided with a lifting base and a rotary disk arranged on the lifting base.

As a further optimization of the above enrichment device:

the ultrasonic treatment part of the treatment rod body is a stainless steel rod body, one end of the ultrasonic treatment part of the treatment rod body is connected with the ultrasonic generator, and the other end of the ultrasonic treatment part of the treatment rod body is connected with the electromagnet adsorption part.

As a further optimization of the above enrichment device:

the liquid bearing implement has:

the base can be temporarily fixed on the table top of the oscillator through the clamping seat; and

a first treatment canister; and

a second treatment canister;

a first processing section of thick bamboo and second are handled a section of thick bamboo and are all installed on the base through detachable mode.

As a further optimization of the above enrichment device:

the first treatment cylinder and the second treatment cylinder have different appearance colors; or

The wall of the first treatment cylinder and the wall of the second treatment cylinder are provided with different marks.

As a further optimization of the above enrichment device:

and stirring units are arranged at the bottoms of the first treatment cylinder and the second treatment cylinder.

As a further optimization of the above enrichment device:

the magnetic bead feeder includes:

the magnetic bead storage box is used for storing the magnetic beads to be used and is provided with a discharge hole; and

the quantitative feeder is arranged at the discharge port of the magnetic bead storage box.

As a further optimization of the above enrichment device:

the magnetic bead is provided with an iron oxide core, a silane layer wraps the outside of the iron oxide core, and the diameter of the magnetic bead is 20-500 nm.

The enrichment device can realize the full-automatic operation of extracting nucleic acid by magnetic beads, and combines the ultrasonic dispersion component and the adsorption component of the magnetic beads, thereby realizing the cross effect of functions and optimizing the structural design of the device.

Drawings

FIG. 1 is a schematic view of the internal structure of the enriching apparatus (initial operation state) according to the present invention;

FIG. 2 is a schematic view showing the internal structure of the enriching apparatus (the first treatment tank is in operation) according to the present invention;

FIG. 3 is a schematic diagram of the internal structure of the enrichment apparatus (after the work positions of the first treatment cylinder and the second treatment cylinder are converted) according to the present invention;

FIG. 4 is a schematic view showing the internal structure of the enriching apparatus (second treating cylinder is in operation) according to the present invention;

FIG. 5 is a schematic view showing the internal structure of a first treatment tank in the enriching apparatus according to the present invention;

FIG. 6 is a schematic view showing the external structure of a first treatment tank in the enriching apparatus according to the present invention;

FIG. 7 is a schematic diagram of the construction of the base of the liquid bearing vessel of the enrichment apparatus of the present invention;

FIG. 8 is a schematic view of the connection between the liquid carrying implement and the oscillator in the enrichment apparatus of the present invention;

FIG. 9 is a schematic view showing the structure of a treatment rod body (ultrasonic treatment part is a straight rod shape) in the enrichment apparatus of the present invention;

FIG. 10 is a schematic view of the structure of the treatment rod body (the ultrasonic treatment part is wavy) in the enrichment apparatus of the present invention;

the labels in the figure are:

1, a box body is arranged in the box body,

2 a liquid-carrying means for carrying a liquid,

201 the base of the device is provided with a base,

202 of the first treatment canister,

203 a second treatment canister,

204 a blind hole is formed in the base plate,

205 is inserted into the hole, and the hole is inserted into the hole,

206 are inserted into the columns in a plugging way,

207 a motor for stirring the mixture and a stirring motor,

208 the blades are stirred in such a way that,

3, an oscillator is used for generating a signal,

4, rotating the lifting platform to move the lifting platform,

401 the base is lifted and lowered in a vertical direction,

402 rotating the disk in a rotating manner,

5 a magnetic bead feeder, wherein the magnetic bead feeder is provided with a magnetic bead feeder,

501 a magnetic bead storage tank, wherein the magnetic bead storage tank,

502 a quantitative feeder, and a quantitative feeding device,

6 a handling rod assembly for the treatment of the animal,

601 the treatment rod body is processed by a treatment rod,

602 an ultrasonic generator for generating ultrasonic waves,

603 an ultrasonic wave processing unit for performing ultrasonic wave processing,

604 an electromagnet attracting portion.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

Referring to FIGS. 1-3: the utility model provides a medical science inspection is with high-efficient enrichment facility of air microorganism based on magnetic bead technique, its essential element includes box 1, carrier liquid utensil 2, oscillator 3, rotatory elevating platform 4, magnetic bead feeder 5, processing rod subassembly 6 and control unit.

< case >

The housing 1 is used for supporting and protecting other components, and the components (the carrier liquid device 2, the oscillator 3, the rotary elevating table 4, the magnetic bead supply device 5, the processing rod assembly 6, and the control unit) are directly or indirectly mounted on the housing 1.

Wherein, rotatory elevating platform 4 sets up the bottom in box 1, and oscillator 3 fixes on rotatory elevating platform 4, and carrier liquid utensil 2 is installed on oscillator 3 when operating condition, handles excellent subassembly 6 and fixes in oscillator 3 top, and magnetic bead feeder 5 sets up the oblique top at oscillator 3, and the control unit is the intelligent unit of device, and its position on the physical structure is not important, can select according to the interior structural design of box 1.

A main power supply is arranged in the box body 1 and provides electric energy for the work of the whole device. Meanwhile, the box body 1 is also provided with a main switch, and after the main switch is turned on, a subsequent series of operations can be carried out. When the main switch is closed, the whole device is in a power-off state.

The front of box 1 should be provided with the chamber door, and the chamber door passes through the hinge mount on box 1, also can set up magnetism between chamber door and the box 1 and inhale the part, when the chamber door was closed, need exert certain power, could open the chamber door. For the convenience of observation, a glass window is arranged on the box door. Furthermore, the observation effect is prevented from being influenced by weak light. An illumination lamp may be provided inside the case 1.

< liquid carrying means >

The carrier liquid appliance 2 is used for loading the processing liquid and is provided with two carrier liquid chambers which are isolated from each other. Specifically, the liquid bearing implement 2 has the following structure:

the liquid bearing implement 2 is composed of a base 201, a first treatment cartridge 202, and a second treatment cartridge 203. The base 201 may be temporarily fixed on the table of the oscillator 3 by a snap structure. The temporary fixing of the working position of the liquid carrying appliance 2 can be realized through the structure.

Wherein, the first treatment cylinder 202 and the second treatment cylinder 203 are detachably mounted on the base 201, so that the first treatment cylinder 202 and the second treatment cylinder 203 can be taken down from the base 201 individually for subsequent treatment, for example, after the enrichment is completed, the second treatment cylinder 203 needs to be removed for subsequent nucleic acid desorption operation. For example, both the first treatment canister 202 and the second treatment canister 203 may need to be cleaned and filled with new treatment fluid. The above operations require that the first treatment canister 202 and the second treatment canister 203 be separately removed from the base 201.

It is contemplated that the connection between the first and second treatment canisters 202 and 203 and the base 201 and the connection between the base 201 and the top of the oscillator 3 may be the same, and that a detachable configuration is required, and that many types of detachable configurations are possible in the prior art.

This embodiment simply describes several specific structures that can be realized:

can dismantle the connection through the structure realization that connects soon between a first processing section of thick bamboo 202 and a second processing section of thick bamboo 203 and the base 201, be provided with two blind holes 204 on base 201 side by side, the blind hole 204 inner wall is provided with the internal thread, the bottom outer wall correspondence of a first processing section of thick bamboo 202 and a second processing section of thick bamboo 203 is provided with the external screw thread, can establish a first processing section of thick bamboo 202 and a second processing section of thick bamboo 203 soon on base 201 through the cooperation of internal thread and external screw thread.

The bottom of the base 201 is provided with a plurality of plug holes 205, the table top of the oscillator 3 is correspondingly provided with a plurality of plug columns 206, when the plug holes 205 of the base 201 are all plugged into the plug columns 206 on the table top of the oscillator 3, the temporary fixation of the base 201 and the table top of the oscillator 3 is completed, at this time, when the oscillator 3 is lifted or rotated, the base 201 and the first treatment cylinder 202 and the second treatment cylinder 203 which are arranged on the base 201 can move along with the oscillator 3, when the base 201 needs to be taken down, the base 201 is pulled out upwards, in order to ensure that the base 201 and the oscillator 3 have a stable connection relationship after the temporary fixation, the inner wall of the plug holes 205 can be further provided with a plurality of elastic materials so as to increase the friction force between the plug holes 205 and the plug columns 206.

Since the first treatment canister 202 and the second treatment canister 203 have different functions and the first treatment canister 202 and the second treatment canister 203 are displaced during the treatment process, the following technical means can be adopted to avoid confusing the first treatment canister 202 and the second treatment canister 203:

the first scheme is as follows: the first treatment cylinder 202 and the second treatment cylinder 203 have different appearance colors, and can be quickly distinguished according to the colors, wherein the first treatment cylinder 202 is one of the first treatment cylinders, and the second treatment cylinder 203 is the other one of the second treatment cylinders.

Scheme II: different marks are arranged on the wall of the first treatment cylinder 202 and the wall of the second treatment cylinder 203, the specific marks can be numbers 1 and 2, and the first treatment cylinder 202 and the second treatment cylinder 203 can be distinguished quickly through the difference of the marks.

In order to further improve the treatment effect, stirring units are arranged at the bottoms of the first treatment cylinder 202 and the second treatment cylinder 203. The stirring unit comprises a stirring motor 207 and stirring blades 208, a base is arranged at the bottom of the treatment cylinder, the motor is arranged in the base, a stirring shaft of the stirring motor 207 extends into the bottom of the treatment cylinder from bottom to top, and the stirring shaft is provided with the stirring blades 208.

In order to optimize the structure of the whole device, the treatment barrel does not carry a power supply, but supplies power through the main power supply of the device, the structures matched with each other are arranged on the table-boards of the base 201 and the oscillator 3, and the structures matched with each other are also arranged between the treatment barrel and the base 201, after the treatment barrel is arranged on the base 201, the conduction of the power supply lines of the treatment barrel and the oscillator 3 is realized, when the base 201 and the table-board of the oscillator 3 are connected together through the matching between the plug-in post 206 and the plug-in hole 205, the connection of the power supply lines and the control lines is realized, and a plurality of schemes capable of realizing the structures in the prior art are provided, and are not described in detail herein. After the base 201 is fixed on the table of the oscillator 3, the start and stop of the stirring unit can be controlled by the general controller of the device.

It should be further noted that, the first treatment cylinder 202 and the second treatment cylinder 203 should be equipped with covers, the covers are connected with the cylinder body of the treatment cylinder through connecting wires, when the cylinder opening needs to be closed, the covers are screwed or clamped to the cylinder opening of the treatment cylinder, especially, the second treatment cylinder 203 after treatment is completed, and the cylinder opening needs to be closed in time after being taken out and transferred to the next operation procedure, so as to avoid the pollution risk in the transfer process.

< Oscillator >

The oscillator 3 is used for driving the liquid carrier device 2 to make an oscillating motion. The oscillator 3 is a device commonly used in experiments, and functions to generate reciprocating oscillation or rotary oscillation for oscillating a target liquid in an instrument such as a test tube placed on the oscillator 3. The oscillator 3 in the present embodiment may be any oscillator 3 in the related art, and a detailed configuration of the oscillator 3 will not be described here.

It should be further noted that in the present embodiment, an oscillator 3 capable of generating reciprocating oscillation is selected, the oscillator 3 can drive the table top of the oscillator 3 to perform reciprocating oscillation, and the amplitude is not too large, and further, the liquid carrier 2 mounted on the table top of the oscillator 3 can follow the table top to perform reciprocating oscillation to perform oscillation treatment on the treatment liquid in the first treatment cylinder 202 and the second treatment cylinder 203.

< rotating elevating platform >

The rotary lifting platform 4 is used for driving the oscillator 3 to perform lifting or rotating motion. The mechanical structure capable of generating two different displacements is very numerous and can be suitably selected according to the state of the art, and the rotary elevating table 4 in this embodiment has an elevating base 401 and a rotating disk 402 provided on the elevating base 401. Wherein, the lifting base 401 is used for realizing reciprocating displacement in the vertical direction, and the rotating disc 402 is used for reciprocating rotation. Corresponding to a simple stacking together of instruments capable of producing both displacements. This is a structural form that can be expected to be comparatively simple.

It should be noted that, in general, the vertical displacement and the rotational displacement do not occur simultaneously, but occur sequentially, for example, first move downward, then rotate, and finally move upward.

< magnetic bead feeder >

The magnetic bead feeder 5 has two functions, one is for storing magnetic beads, and the other is for quantitatively feeding the magnetic beads to the carrier liquid device 2.

The magnetic bead feeder 5 specifically includes: a magnetic bead storage tank 501 and a quantitative feeder 502. Wherein, magnetic bead storage tank 501 is used for storing the magnetic bead for use, has a discharge gate. The quantitative feeder 502 is arranged at the discharge port of the magnetic bead storage tank 501.

The quantitative feeder 502 can be a feeder mature in the prior art, and the amount of each feeding can be set arbitrarily within a controllable range.

The magnetic bead is provided with an iron oxide core, a silane layer wraps the outside of the iron oxide core, and the diameter of the magnetic bead is 20-500 nm.

< treatment Bar Assembly >

The processing rod assembly 6 is a core component of the enrichment device, and the specific structure comprises: the ultrasonic treatment apparatus includes a treatment rod body 601 provided above an oscillator 3, the treatment rod body 601 including an ultrasonic treatment section 603 and an electromagnet attachment section 604, an ultrasonic generator 602 for supplying ultrasonic energy to the ultrasonic treatment section 603, and a power supply circuit for driving the electromagnet attachment section 604.

The ultrasonic treatment part 603 of the treatment rod body 601 is a stainless steel rod body, one end of the ultrasonic treatment part 603 of the treatment rod body 601 is connected with the ultrasonic generator 602, and the other end is connected with the electromagnet adsorption part 604.

The ultrasonic generator is a common structure of an ultrasonic processing device, is not innovated by the applicant, and has the innovation points that: an electromagnet is arranged on the rod body capable of generating ultrasonic treatment, and the electromagnet can be switched between a magnetic state and a non-magnetic state by electrifying.

Through above-mentioned structural design for handle the stick and collect two kinds of functions in one of ultrasonic treatment and the adsorption treatment of magnetic bead, and ultrasonic treatment can also be from the time and the effect of the in-process acceleration desorption of electro-magnet desorption at the magnetic bead, has played synergistic effect.

< control means >

The control unit is used for controlling the oscillator 3, the rotary lifting table 4 and the processing rod assembly 6, and is an intelligent core of the whole device, and the following control logics are required to be realized through the control unit:

when the liquid-carrying appliance 2 is properly mounted on the table of the oscillator 3, the start button is clicked, as deemed appropriate.

S1 and < rotary stage 4> receive the command, move upward, and finally move the < liquid carrying means 2> upward, so that the lower part of the < treatment rod assembly 6> extends into the first treatment canister 202.

And S2, when the instruction of S1 is completed, the instruction is sent to < oscillator 3> to perform oscillation processing, and oscillation is stopped after oscillation is completed.

And S3, when the instruction of S2 is completed, sending an instruction to the < processing rod assembly 6>, carrying out ultrasonic dispersion processing, and stopping ultrasonic processing after ultrasonic dispersion is finished.

S4, when the command of S3 is completed, the command is issued to the < magnetic bead feeder 5>, and a predetermined amount of magnetic beads are loaded into the first processing cylinder 202.

And S5, when the instruction of S4 is completed, sending an instruction to the < processing rod assembly 6>, carrying out ultrasonic dispersion processing, and stopping ultrasonic processing after ultrasonic dispersion is finished.

S6, when the command of S5 is completed, the command is issued to < treatment rod assembly 6> and < first treatment tank 202>, the electromagnet portion of < treatment rod assembly 6> is made magnetic, and the stirring by the stirring means of < first treatment tank 202> is started, and after the treatment is completed, the stirring is stopped while the electromagnet portion of < treatment rod assembly 6> is kept magnetic.

S7, when the command of S6 is completed, the command is issued to the < rotary lift table 4>, and the processing rod assembly is moved downward, rotated so that the < second processing cylinder 203> is positioned right below the < processing rod assembly 6>, and then moved upward so that the lower portion of the < processing rod assembly 6> is inserted into the < second processing cylinder 203 >.

And S8, after the instruction of S7 is completed, sending an instruction to the treatment rod assembly 6 to enable the electromagnet part of the treatment rod assembly 6 to lose magnetism, simultaneously starting the ultrasonic part of the treatment rod assembly 6 to perform ultrasonic dispersion, and stopping ultrasonic treatment after the ultrasonic dispersion is finished.

At S9, when the command at S8 is completed, the command is issued to the < rotary stage 4>, and the table is moved downward.

The control logic is the operation logic of the enrichment apparatus of the present invention during operation, and can be realized through appropriate processor selection and programming of programs, and will not be described in detail herein.

< other problems to be explained >

1. < first treatment canister 202> and < second treatment canister 203> it is necessary to fill the canisters with an acidic buffer before treatment.

2. The enrichment apparatus of the present invention extracts the target substance from the filter membrane on which the air sample is adsorbed, and before the treatment, the filter membrane on which the air sample is adsorbed is crushed and put in the first treatment cylinder 202.

3. The second processing cylinder 203 processed by the enrichment device of the invention is rich in magnetic beads absorbed with target substances, and then magnetic beads can be obtained by centrifugal separation for extracting DNA.

The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes and modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention.