阅读说明：本技术 一种老年犬营养膏及其制备方法和应用 (Nutritional paste for old dogs and preparation method and application thereof ) 是由 吴怡 胡金华 张泽宇 彭晓培 马东立 郑美大 夏黎明 于 2020-05-29 设计创作，主要内容包括：本发明提供了一种老年犬营养膏,包含：6.0至19.0重量份的功能卵磷脂、8至16重量份的蛋白质、3至10重量份的风味诱食料、1.11至8.4重量份的氨基酸类组分、0.031至0.305重量份的维生素、0.1至3重量份的矿物质、0.01至0.5重量份的有机酸、1.1至8重量份的油脂、0.005至0.01重量份的功能寡糖、30至55重量份的载体、0.1至0.6重量份的着色剂、0.5至1.5重量份的增稠剂和10至30重量份的水。本发明还提供了所述营养膏的应用和制备方法。本发明营养膏能够增进食欲,促进消化吸收,防止腹泻和排便异常,避免过敏反应,促使毛发柔顺有光泽等。(The invention provides a nutritional paste for old dogs, which comprises the following components: 6.0 to 19.0 parts by weight of functional lecithin, 8 to 16 parts by weight of protein, 3 to 10 parts by weight of flavor inducing material, 1.11 to 8.4 parts by weight of amino acid component, 0.031 to 0.305 parts by weight of vitamin, 0.1 to 3 parts by weight of mineral, 0.01 to 0.5 parts by weight of organic acid, 1.1 to 8 parts by weight of fat and oil, 0.005 to 0.01 parts by weight of functional oligosaccharide, 30 to 55 parts by weight of carrier, 0.1 to 0.6 parts by weight of colorant, 0.5 to 1.5 parts by weight of thickener and 10 to 30 parts by weight of water. The invention also provides application and a preparation method of the nutritional paste. The nutritional paste can promote appetite, promote digestion and absorption, prevent diarrhea and abnormal defecation, avoid anaphylactic reaction, promote hair to be smooth and glossy and the like.)

1. The nutritional paste for the aged dogs comprises the following components or consists of the following components:

6.0 to 19.0 parts by weight of functional lecithin, 8 to 16 parts by weight of protein, 3 to 10 parts by weight of flavor inducing material, 1.11 to 8.4 parts by weight of amino acid component, 0.031 to 0.305 parts by weight of vitamin, 0.1 to 3 parts by weight of mineral, 0.01 to 0.5 parts by weight of organic acid, 1.1 to 8 parts by weight of fat and oil, 0.005 to 0.01 parts by weight of functional oligosaccharide, 30 to 55 parts by weight of carrier, 0.1 to 0.6 parts by weight of colorant, 0.5 to 1.5 parts by weight of thickener and 10 to 30 parts by weight of water.

2. The nutritional paste of claim 1, wherein:

the functional lecithin is one or more components selected from the group consisting of egg yolk powder and soybean lecithin;

the protein is selected from one or more of the group consisting of chicken protein powder and chicken meal;

the flavor attractant is selected from one or more of the group consisting of chicken liver powder and chicken soluble paste;

the amino acid component is selected from one or more of the group consisting of taurine, cystine and lysine;

the vitamins are selected from one or more of the group consisting of vitamin B1, vitamin B2, vitamin B12 and vitamin E;

the mineral substance is calcium hydrophosphate;

the organic acid is lactic acid;

the grease is selected from one or more of the group consisting of deep sea fish oil and linseed oil;

the fructo-oligosaccharide is fructo-oligosaccharide;

the carrier is maltose syrup;

the colorant is caramel color; and/or

The thickening agent is sodium carboxymethyl cellulose.

3. The nutritional paste of claim 1 or 2, wherein:

the functional lecithin is composed of egg yolk powder and soybean lecithin;

the protein consists of chicken protein powder and chicken powder;

the flavor inducing food is composed of chicken liver powder and chicken soluble paste;

the amino acid component consists of taurine, cystine and lysine;

the vitamins consist of vitamin B1, vitamin B2, vitamin B12 and vitamin E;

the mineral substance is calcium hydrophosphate;

the organic acid is lactic acid;

the grease consists of deep sea fish oil and linseed oil;

the fructo-oligosaccharide is fructo-oligosaccharide;

the carrier is maltose syrup;

the colorant is caramel color; and/or

The thickening agent is sodium carboxymethyl cellulose.

4. The nutritional paste according to any one of claims 1 to 5, wherein:

when present, the content of each component is as follows in parts by weight:

5. the nutritional paste according to any one of claims 1 to 4, wherein:

the nutritional paste for the aged dogs further comprises a preservative and/or a flavoring agent; preferably, the preservative is potassium sorbate, and/or the flavoring agent is sodium chloride; more preferably, the nutrient paste for the aged dog further comprises 0 to 0.2 parts by weight of potassium sorbate and/or 0 to 0.5 parts by weight of sodium chloride, and further preferably further comprises 0.1 to 0.2 parts by weight of potassium sorbate and/or 0.01 to 0.5 parts by weight of sodium chloride.

6. The nutritional paste according to any one of claims 1 to 5, wherein:

the nutritional paste for the aged dogs further comprises a plant extract, wherein the plant extract is a hawthorn extract and/or an astragalus extract; preferably, the nutritional paste for the aged dog further comprises 0.01 to 0.1 part by weight of hawthorn extract and/or 0.01 to 0.08 part by weight of astragalus extract.

7. The nutritional paste according to any one of claims 1 to 7, wherein:

the senior dog nutritional paste comprises one or more selected from the group consisting of kelp extract, wolfberry extract, fish collagen and hyaluronic acid.

8. The nutritional paste according to any one of claims 1 to 7, wherein:

the nutritional paste for senior dogs comprises 0.01 to 1.5 parts by weight of kelp extract, 0.01 to 0.1 part by weight of wolfberry extract, 0.01 to 1 part by weight of fish collagen, and 0.01 to 0.1 part by weight of hyaluronic acid.

9. An application of a nutritional paste for senior dogs in feeding or preparing feed for senior dogs, wherein:

the senior dog nutrition paste comprises 0 to 1.5 parts by weight of kelp extract, 0 to 0.1 parts by weight of wolfberry extract, 0 to 1 parts by weight of fish collagen, and 0 to 0.1 parts by weight of hyaluronic acid, and the senior dog nutrition paste comprises at least one, at least two, or all three selected from among kelp extract, wolfberry extract, fish collagen, and hyaluronic acid; preferably, the senior dog nutritional paste comprises 0.01 to 1.5 parts by weight of kelp extract, 0.01 to 0.1 parts by weight of wolfberry extract, 0.01 to 1 parts by weight of fish collagen, and 0.01 to 0.1 parts by weight of hyaluronic acid; or

The senior dog nutrition paste as recited in any one of claims 1 to 8.

10. A method of preparing the senior dog nutritional paste of any one of claims 1 to 8, wherein the method comprises the steps of:

(1) mixing the carrier, the functional oligosaccharide and water to prepare carrier syrup;

(2) adding optional fructus Lycii extract, optional fish collagen, yolk powder, protein, flavor inducing material and optional antiseptic, stirring and homogenizing to obtain mixture; in addition, homogenizing, stirring and heating the deep sea fish oil and the linseed oil to 75-80 ℃ to obtain mixed oil;

(3) adding optional soybean lecithin and thickener into the mixture, homogenizing and emulsifying, slowly dropping the mixed oil for three times, homogenizing and emulsifying to obtain a first emulsion;

(4) adding organic acid, mineral substances, sodium chloride, amino acid components, vitamin components, optional kelp extract and optional hyaluronic acid into the first emulsion, uniformly mixing, adding a coloring agent for color mixing, stirring and emulsifying to obtain a second emulsion, and cooling to room temperature to form a paste serving as a nutritional paste;

preferably, the method further comprises the steps of: (5) sterilizing the paste by a magnetic sterilization method; and (6) filling and sealing the sterilized paste, and performing secondary sterilization to obtain the nutritional paste product.

Technical Field

The invention relates to the technical field of pet food preparations, in particular to a nutritional paste for old dogs and a preparation method and application thereof.

Background

With the improvement of living standard of people, the need of mental level is more and more emphasized. Thus, pets, and in particular pet dogs, have long been an essential life partner for many families. However, the life of a pet dog is far shorter than that of a human, and thus many pet dogs are now stepping into an aging stage in homes. Various health problems in older dogs follow, such as the beginning of a decline in metabolism and immune system, a decrease in intestinal absorption, an appearance in which eyebrows and hair in the nose area of the dog become pale, and joint aging even makes walking difficult.

Since pet dogs are required for the pursuit of human mental life, family members often have a profound feeling in senior dogs, because the raising and health problems of senior dogs are receiving more and more attention.

CN201810474637.2 provides a health care nutrition paste for senior pets, which comprises the following raw material components: astragalus extract, ginseng extract, schisandra extract, ginkgo biloba extract, angelica extract, wolfberry extract, chondroitin sulfate, glucosamine hydrochloride, spray-dried porcine plasma protein powder, chicken hydrolyzed paste, glucose, fructooligosaccharide, lecithin, sodium carboxymethylcellulose, fish oil, evening primrose seed oil, walnut kernel oil, sodium benzoate, zinc sulfate, copper sulfate pentahydrate, magnesium sulfate and taurine. The health care nutrition paste for the aged pets mainly utilizes the traditional Chinese medicine extract contained in the paste to delay aging and protect viscera and joints.

Aiming at the problems of poor appetite, poor digestion and absorption, easy diarrhea, easy anaphylactic reaction, poor fur and the like of old dogs, the inventor provides a special nutritional paste for the old dogs through long-term research, and provides a preparation method and application of the nutritional paste.

Disclosure of Invention

In order to solve one or more of the above technical problems in the prior art, the present invention provides, in a first aspect, a nutritional paste for senior dogs, wherein the nutritional paste comprises or consists of the following components:

6.0 to 19.0 parts by weight of functional lecithin, 8 to 16 parts by weight of protein, 3 to 10 parts by weight of flavor inducing material, 1.11 to 8.4 parts by weight of amino acid component, 0.031 to 0.305 parts by weight of vitamin, 0.1 to 3 parts by weight of mineral, 0.01 to 0.5 parts by weight of organic acid, 1.1 to 8 parts by weight of oil and fat, 0.005 to 0.01 parts by weight of functional oligosaccharide, 30 to 55 parts by weight of carrier, 0.1 to 0.6 parts by weight of colorant, 0.5 to 1.5 parts by weight of thickener and 10 to 30 parts by weight of water

The present invention provides in a second aspect the use of a senior dog nutrition paste comprising 0 to 1.5 parts by weight of kelp extract, 0 to 0.1 parts by weight of wolfberry extract, 0 to 1 parts by weight of fish collagen and 0 to 0.1 parts by weight of hyaluronic acid in feeding to senior dogs or preparing feed for senior dogs, and the senior dog nutrition paste comprising at least one, at least two or all three selected from the group consisting of kelp extract, wolfberry extract, fish collagen and hyaluronic acid; or the nutritional paste for the senior dog is the nutritional paste for the senior dog in the first aspect of the invention.

The present invention also provides in a third aspect a method of preparing a nutritional paste according to the first aspect of the invention, the method comprising:

(1) mixing the carrier, the functional oligosaccharide and water to prepare carrier syrup;

(2) adding optional fructus Lycii extract, optional fish collagen, yolk powder, protein, flavor inducing material and optional antiseptic, stirring and homogenizing to obtain mixture; in addition, homogenizing, stirring and heating the deep sea fish oil and the linseed oil to 75-80 ℃ to obtain mixed oil;

(3) adding optional soybean lecithin and thickener into the mixture, homogenizing and emulsifying, slowly dropping the mixed oil for three times, homogenizing and emulsifying to obtain a first emulsion;

(4) adding organic acid, mineral substances, sodium chloride, amino acid components, vitamin components, optional kelp extract and optional hyaluronic acid into the first emulsion, uniformly mixing, adding a coloring agent for color mixing, stirring and emulsifying to obtain a second emulsion, and cooling to room temperature to form a paste serving as a nutritional paste;

compared with the prior art, the invention has the following technical advantages: can remarkably promote the appetite of the old dogs, promote digestion and absorption, prevent diarrhea and abnormal defecation, avoid anaphylactic reaction, promote hair to be smooth and glossy and the like.

Drawings

Figure 1 selection results of nutritional cream taste tests for senior dogs.

FIG. 2 the effect of the nutritional cream for senior dogs on the body weight of test dogs.

FIG. 3 the effect of the nutritional paste for senior dogs on the average daily food intake of test dogs.

FIG. 4 shows the effect of the nutritional cream for senior dogs on the state of coat hair of test dogs in 10 days.

FIG. 5 shows the effect of the nutritional cream for senior dogs on the state of coat hair of test dogs for 20 days.

FIG. 6 shows the effect of the nutritional cream for senior dogs on the state of coat hair of test dogs for 30 days.

Detailed Description

In order to solve one or more of the above technical problems in the prior art, the present invention provides, in a first aspect, a nutritional paste for senior dogs, wherein the nutritional paste comprises or consists of the following components:

6.0 to 19.0 parts by weight (e.g., 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 or 18 parts by weight) of a functional lecithin, 8 to 16 parts by weight ((e.g., 9, 10, 11, 12, 13, 14 or 15 parts by weight)) of a protein, 3 to 10 parts by weight (e.g., 3, 4, 5, 6, 7, 8, 9 or 10 parts by weight) of a flavor-inducing food material, 1.11 to 8.4 parts by weight (e.g., 2, 3, 4, 5, 6, 7 or 8 parts by weight) of an amino acid-based component, 0.031 to 0.305 parts by weight (e.g., 0.05, 0.1, 0.2 or 0.3 parts by weight) of a vitamin, 0.1 to 3 parts by weight (e.g., 0.5, 1.0 or 2.0 parts by weight) of a mineral, 0.01 to 0.5 parts by weight (e.01, 0.02 or 0.5 parts by weight) of an organic acid, 1.01 to 0.8 parts by weight (e.01 to 0.005 parts by weight) of an oligosaccharide, 8 parts by weight (e.01 to 0.8 parts by weight) of an oligosaccharide, 0.1.1.1 part by weight or 8 parts by weight (e.01 parts by weight) of an oligosaccharide, 8 parts by weight) of an oil or 0.1.1 part by weight of an oligosaccharide, 005, 0 part by weight of a functional oil or 10 part by weight, 30 to 55 parts by weight (e.g., 35, 40, 45, or 50 parts by weight) of a carrier, 0.1 to 0.6 parts by weight (e.g., 0.1, 0.3, or 0.6 parts by weight) of a colorant, 0.5 to 1.5 parts by weight (e.g., 0.5, 1.0, or 1.5 parts by weight) of a thickener, and 10 to 30 parts by weight (e.g., 10, 15, 20, or 25 parts by weight) of water.

In other preferred embodiments, the functional lecithin comprises one or more components selected from the group consisting of egg yolk powder and soy lecithin; the protein comprises one or more selected from the group consisting of chicken protein powder and chicken meal; the flavor attractant comprises one or more selected from the group consisting of chicken liver powder and chicken plasma; the amino acid component comprises one or more selected from the group consisting of taurine, cystine, and lysine; the vitamins comprise one or more selected from the group consisting of vitamin B1, vitamin B2, vitamin B12, and vitamin E; the mineral comprises dibasic calcium phosphate; the organic acid comprises lactic acid; the fats and oils comprise one or more selected from the group consisting of deep sea fish oil and linseed oil; the fructooligosaccharide comprises fructooligosaccharide; the carrier comprises maltose syrup; the colorant comprises caramel color; and/or the thickener comprises sodium carboxymethylcellulose.

In other preferred embodiments, the functional lecithin is selected from one or more components of the group consisting of egg yolk powder and soy lecithin; the protein is selected from one or more of the group consisting of chicken protein powder and chicken meal;

the flavor attractant is selected from one or more of the group consisting of chicken liver powder and chicken soluble paste; the amino acid component is selected from one or more of the group consisting of taurine, cystine and lysine; the vitamins are selected from one or more of the group consisting of vitamin B1, vitamin B2, vitamin B12 and vitamin E; the mineral substance is calcium hydrophosphate; the organic acid is lactic acid; the grease is selected from one or more of the group consisting of deep sea fish oil and linseed oil; the fructo-oligosaccharide is fructo-oligosaccharide; the carrier is maltose syrup; the colorant is caramel color; and/or the thickener is sodium carboxymethylcellulose.

In other preferred embodiments, the functional lecithin is comprised of egg yolk powder and soy lecithin; the protein consists of chicken protein powder and chicken powder; the flavor inducing food is composed of chicken liver powder and chicken soluble paste; the amino acid component consists of taurine, cystine and lysine; the vitamins consist of vitamin B1, vitamin B2, vitamin B12 and vitamin E; the mineral substance is calcium hydrophosphate; the organic acid is lactic acid; the grease consists of deep sea fish oil and linseed oil; the fructo-oligosaccharide is fructo-oligosaccharide; the carrier is maltose syrup; the colorant is caramel color; and/or the thickener is sodium carboxymethylcellulose.

In other preferred embodiments, the amounts of the components, when present, in parts by weight are as follows:

in other preferred embodiments, the senior dog nutrition paste further comprises a preservative and/or a flavoring agent; preferably, the preservative is potassium sorbate, and/or the flavoring agent is sodium chloride; more preferably, the nutrient paste for the aged dog further comprises 0 to 0.2 parts by weight of potassium sorbate and/or 0 to 0.5 parts by weight of sodium chloride, and further preferably further comprises 0.1 to 0.2 parts by weight of potassium sorbate and/or 0.1 to 0.5 parts by weight of sodium chloride.

In other preferred embodiments, the senior dog nutrition paste comprises one or more selected from the group consisting of kelp extract, wolfberry extract, fish collagen and hyaluronic acid; preferably, the senior dog nutritional paste comprises 0.01 to 1.5 parts by weight of kelp extract, 0.01 to 0.1 parts by weight of wolfberry extract, 0.01 to 1 parts by weight of fish collagen, and 0.01 to 0.1 parts by weight of hyaluronic acid.

The feeding method of the nutrient paste is not particularly limited, and can be carried out in the following way: (1) direct smearing: can be applied to nose or mouth of dog for licking. (2) Feeding the daily ration: can be mixed with daily ration for feeding, and the addition amount in the complete pet food is not more than 10%. (3) Feeding in a dosing manner: the aged dogs with recovery or poor appetite can be fed with appropriate dosage.

The feeding amount is not particularly limited in the present invention, but it is preferable to provide corresponding daily feeding amounts according to different body weights: 1-2 kg: about 1-3 g; 3-4 kg: about 4-6 g; 5-6 kg: about 7-8 g; 6kg above: about 8 g. The feeding amount can be adjusted according to the age, weight and activity of pets.

The present invention provides in a second aspect the use of a senior dog nutrition paste comprising 0 to 1.5 parts by weight of kelp extract, 0 to 0.1 parts by weight of wolfberry extract, 0 to 1 parts by weight of fish collagen and 0 to 0.1 parts by weight of hyaluronic acid in feeding to senior dogs or preparing feed for senior dogs, and the senior dog nutrition paste comprising at least one, at least two or all three selected from the group consisting of kelp extract, wolfberry extract, fish collagen and hyaluronic acid; preferably, the senior dog nutritional paste comprises 0.01 to 1.5 parts by weight of kelp extract, 0.01 to 0.1 parts by weight of wolfberry extract, 0.01 to 1 parts by weight of fish collagen, and 0.01 to 0.1 parts by weight of hyaluronic acid.

In other preferred embodiments, the senior dog nutrition paste is the senior dog nutrition paste of the first aspect of the present invention.

The present invention also provides in a third aspect a method of preparing a nutritional paste according to the first aspect of the invention, the method comprising:

(1) mixing the carrier, the functional oligosaccharide and water to prepare carrier syrup;

(2) adding optional fructus Lycii extract, optional fish collagen, yolk powder, protein, flavor inducing material and optional antiseptic, stirring and homogenizing to obtain mixture; in addition, homogenizing, stirring and heating the deep sea fish oil and the linseed oil to 75-80 ℃ to obtain mixed oil;

(3) adding optional soybean lecithin and thickener into the mixture, homogenizing and emulsifying, slowly dropping the mixed oil for three times, homogenizing and emulsifying to obtain a first emulsion;

(4) adding organic acid, mineral substances, sodium chloride, amino acid components, vitamin components, optional kelp extract and optional hyaluronic acid into the first emulsion, mixing uniformly, adding a coloring agent for color mixing, stirring and emulsifying to obtain a second emulsion, and cooling to room temperature to form a paste serving as the nutritional paste.

Preferably, the method further comprises the steps of: (5) sterilizing the paste by a magnetic sterilization method; and (6) filling and sealing the sterilized paste, and performing secondary sterilization to obtain the nutritional paste product.

In the above method, if the nutritional paste to be prepared does not contain the corresponding component, the component is not added when referring to the component.

The paste can be made into 120 g/bag or a plurality of small packages.

In some preferred embodiments, the nutritional paste comprises hawthorn extract, which has the effects of inhibiting thyroid peroxidase, resisting cancer, lowering blood pressure, resisting bacteria, activating blood circulation, reducing blood fat, inhibiting platelet aggregation and relieving spasm.

In some preferred embodiments, the nutritional cream comprises an extract of Astragalus membranaceus, which contains flavones in an amount of about 1.16% by weight of the total weight of Astragalus membranaceus, and has effects of dilating blood vessels, improving blood circulation of skin, and protecting liver, wherein the active ingredient flavone has effects of anti-ischemia and improving blood phase, is an important member of the natural antioxidant family, and has strong free radical scavenging and lipid peroxidation inhibiting properties.

In addition, in some preferred embodiments, the nutritional paste contains the wolfberry fruit extract, wherein the content of wolfberry fruit polysaccharide is about 3.6%, and the nutritional paste has the effects of reducing blood fat, resisting fatty liver, resisting aging and the like.

In some more preferred embodiments, the nutritional paste according to the present invention comprises a kelp extract which is rich in various vitamins, minerals and various bioactive organisms and is easily digested and absorbed, and is presumed to be one of the main reasons why the nutritional paste according to the present invention can increase appetite, promote digestion and absorption, promote body growth, and the like.

Examples

The present invention will be further illustrated by the following examples, but the scope of the invention as claimed is not limited to these examples. The starting materials used in the examples are, unless otherwise indicated, conventional starting materials which are commercially available.

Preparation example

The following formula was used in the following table 1, 2kg of each nutritional cream was prepared:

table 1: composition of different formulations

The preparation method is carried out in the following way (if a certain component is not present, the use of the corresponding component is omitted):

(1) feeding in a first group: adding maltose syrup, fructo-oligosaccharide and water into an emulsifying pot. Starting a heating switch of the homogenizing emulsifying machine, and heating to 80 ℃ while stirring to prepare the carrier syrup.

(2) Feeding in a second group: adding fructus crataegi extract (Xian Ruelin Biotechnology limited), fish collagen (Wuhan flourishing Tianyuan Biotechnology limited), egg yolk powder, chicken meat powder, chicken protein powder, chicken liver powder, chicken soluble slurry and potassium sorbate into emulsifying pot, vacuumizing, and stirring for 15 min. Stirring, homogenizing, and slowly heating to 80 deg.C. Meanwhile, adding the deep sea fish oil and the linseed oil into an oil phase pot according to the formula proportion, vacuumizing, stirring, homogenizing, stirring and heating to 75-80 ℃.

(3) Third group of feeding + emulsification 1: adding soybean lecithin and sodium carboxymethylcellulose into the product obtained in the emulsifying pot according to the formula proportion, starting a homogenizing emulsifying machine, vacuumizing until the vacuum degree is 30-100 kPa, slowly dripping the mixed oil body in an oil phase pot into the emulsifying pot for three times until all oil phases are completely and uniformly coated by the water phase, and homogenizing and emulsifying for 4 minutes at 78 ℃ at the stirring speed of 60-300 r/min.

(4) Fourth group feeding + emulsification 2: the product obtained by the method is weighed according to the formula proportion, added with lactic acid, calcium hydrophosphate, sodium chloride, amino acid components (taurine, cystine and lysine), vitamin components (vitamin E, vitamin B1, vitamin B2 and vitamin B12), kelp extract (Xian Purui biological engineering limited) and hyaluronic acid (Wuhan Yimei biological technology limited), added with caramel for color matching, stirred at 78 ℃ for starting emulsification, stopped for emulsification after 5-10 minutes, continuously stirred for 30-40 minutes, and cooled to 45-50 ℃ while stirring.

(5) And (3) sterilization treatment: after the paste is cooled to normal temperature, the paste is sterilized by a magnetic sterilization method, and the magnetic strength is selected to be 0.6 tex.

(6) Filling: and transferring the sterilized paste to a filling line, and filling and sealing under the condition of opening a 2000-air-volume dynamic air sterilizer for 60 minutes (to avoid secondary pollution). The specification of the finished product is 120 g/count.

(7) And (3) sterilizing after filling: and (4) after filling, sterilizing by adopting a pasteurization method (at 70-80 ℃ for 30-40 minutes).

Nutritional pastes 1 to 7 were prepared according to the above formulations 1 to 7, respectively.

Test example 1

42 beagle dogs aged over 2 years are selected in the test, and are divided into 7 groups according to the principle that the weights are similar and the male half and the female half are respectively prepared into 1 to 7 nutritional pastes according to the formulas 1 to 7. The test period is 28 days, blood is collected after the test is finished, and antioxidant indexes (SOD and GSH) and immune indexes (IL-6 and IL-10) in blood plasma are detected. The blood sampling steps are as follows: (1) preparing tourniquet, dry cotton ball, alcohol cotton ball, blood sampling tube, and soft connection type blood sampling needle. (2) Animals were housed. (3) Animal hair was wetted with an alcohol cotton ball, and the wetted hair was separated to expose the approximate location of the blood vessel and disinfected. (4) And (5) tightening the tourniquet to find the blood vessel. (5) And (4) inserting the blood taking needle into the blood vessel, connecting the blood taking tube and loosening the tourniquet when blood flowing out of the blood collecting needle rubber tube is observed. (6) The blood taking needle is pulled out and stopped by a dry cotton ball. (7) The blood sample is sent for detection as soon as possible, and the shorter the storage time of the blood sample, the higher the accuracy. See table 2 for results.

TABLE 2

The test results are shown in the following table. Compared with the nutrient pastes 1 to 4, the nutrient pastes 5 to 7 have higher antioxidant capacity, which is reflected by obviously improving the content of antioxidant SOD in the blood plasma of dogs (P <0.05), and the treatment groups of the nutrient pastes 5 to 7 have no obvious difference (P > 0.10). Compared with other groups, the nutrition paste 5 obviously improves the content of the antioxidant GSH in the blood plasma of dogs (P < 0.05). Compared with the nutrient pastes 1 to 4, the nutrient pastes 5 to 7 have better anti-inflammatory effect, which is reflected in that the content of inflammatory factors IL-6 and IL-10 in the plasma of the dogs is obviously reduced (P <0.05), and no obvious difference exists between the treatment groups of the nutrient pastes 5 to 7 (P > 0.10). In general, the nutritional paste 5 performed best.

Test example 2

First, test procedure

5kg of the nutrient pastes B1 and A1 were prepared in the same manner as in the above preparation examples, respectively, according to the formula 1 and the formula 5 in the above preparation examples.

Grouping condition, dosage and frequency of the nutrient paste:the experimental animals used in the examples were as follows: 15 beagle dogs aged 2 years or older (8 male dogs, 7 female dogs, initial body weight of about 10 kg, initial body weight within 1 kg). The test groups are divided into 3 groups according to the principle that the body weights are similar and the male and female halves are in each group. Group A is basic full-value dog food and A1 nutrition paste; group B is basic full-value dog food + B1 nutrition paste; group C was a basal full-rate dog food + C1 control nutritional cream. Wherein the basic full-value dog food is (ROYAL CANIN Royal dog food A1 preferably senior dog food), and the control nutritional paste is commercially available red pike oil hair-beautifying paste. See table 3 below for details.

Table 3: grouping condition, dosage and frequency of nutrient paste

The feeding mode and test time of the nutrient paste are as follows: the paste was squeezed into the dog mouth directly into the appropriate length using a hand-held nutrition paste packaging tube, daily at 8 am and 16 pm. The test period was 30 days.

The experimental contents are as follows:

15 beagle dogs were raised in a single cage. The body weight was weighed on days 0, 7, 14, 21, 30 at the start of the test. And (3) regularly cleaning excrement, cleaning a dog house, cleaning a water basin, removing the spoon head of the weighing spoon, cleaning and replacing clean drinking water every morning. Recording the state of the excrement during the excrement cleaning. The feed is directly fed to A1 nutrition paste 5g for each dog in A test group, B1 nutrition paste 5g for each dog in B test group and C1 control nutrition paste 5g for each dog in positive control group at 8 am every day, and the feed is fed to 150g of basal full-rate dog food for each dog at 9 am every day. The water is freely drunk all day. 9 in the morning: the inspection is performed for 1 time 30 times, and the contents comprise photographing and recording the fur condition, the skin condition and the mental condition of each dog. Feeding 5g of A1 nutrient paste for each dog in a test group A, 5g of B1 nutrient paste for each dog in a test group B and 5g of C1 control nutrient paste for each dog in a positive control group at 16 pm, feeding 200g of basic full-price dog food for each dog after half an hour, removing the feed basin and weighing the weight of the rest material in the feed basin when the dog turns for the first time and does not eat any more, calculating the daily feed intake of each dog, and counting by 350g of the daily feed intake if the dog finishes all eating once. Dogs were tethered out of the kennel at 17 o' clock each day for 1 hour of appropriate exercise. 7 at night: the tour was 1 time 30 and the content included recording the mental state of the dog.

And simultaneously carrying out comprehensive effect evaluation, carrying out product taste test 3 days before the test, and carrying out 8 o' clock once every morning for three times in total. The nutritional creams of each experimental group or comparative group were prepared in 3 portions each, 5g each, and 9 portions were randomly placed in the same parallel area with 15 cm spacing between each two plates. Each time 1 dog was led to an independent experimental area, the pet dogs were allowed to freely move to feed, and 15 dogs were individually selected. All the operations of the first 3 days are the same as the conventional operations except for the feeding mode of the nutrient paste.

Sampling indexes and methods:

(1) weight: a total of 5 body weights were recorded for each dog.

(2) Food intake: feed intake was recorded 1 total per dog daily.

(3) The situation of the quilt feathers: the condition of the hair of each dog was recorded 1 time by photographing every morning round, and the photograph required that the fullness and glossiness of the hair could be clearly seen.

(4) Skin condition: the skin state of each dog is recorded 1 time every morning round, and the hair on the back, chest, face (near the ear root) and the right neck of each dog is pulled out to observe the abnormal conditions of erythema, erythra, dandruff and the like.

(5) Defecation condition: feces were recorded for each dog 1 time daily in the morning at the time of feces clearance.

(6) Mental state: mental status was recorded 2 times daily for each dog on morning and evening tours.

(7) Selecting a record: each dog was pulled daily in the morning on the induction diet 3 days prior to the trial and the first selected nutrition was recorded. After the traction test was completed, the 8 extra pots were removed and the above routine recording was performed.

Second, test results

Taste selection test for nutritional creams

As shown in table 3, the nutritional pastes of test a and test B were selected significantly more times in three independent taste tests than the positive control. Of the total 45 independent selections, the a1 nutritional cream for senior dogs was selected 25 times to account for 55.6%, the B1 nutritional cream for senior dogs was selected 13 times to account for 28.9%, and the commercial red dog nutritional cream for the positive control group was selected only 7 times to account for 15.6%.

As shown in table 4 and fig. 1, the selection frequency of the a1 senile nutrition cream is significantly higher than that of the B1 senile nutrition cream (P <0.01) and that of the commercial red dog nutrition cream (P <0.01), while the B1 senile nutrition cream has a higher trend but no significant difference (P >0.05) compared with that of the commercial red dog nutrition cream, which indicates that the food calling performance of the a1 senile nutrition cream is significantly better than that of the commercial red dog nutrition cream, and the B1 senile nutrition cream is also better than that of the commercial red dog nutrition cream but does not reach a significant level.

TABLE 3 taste test selection results for nutritional paste for senior dogs

TABLE 4 taste test selection significance analysis for nutritional paste for senior dogs

	Test group A	Test group B	Positive control group	P value
					Average number of selections (times)	8.33±0.88a	4.33±0.67b	2.33±0.33b	0.002

Note: data are presented as mean ± sd, with differences from the shoulder mark indicating significant differences (P < 0.05).

Test of comprehensive Effect

1. Body weight

As can be seen from table 5 and fig. 2, the body weight of the dogs in the test group a gradually increased over the entire test period, the body weight of the dogs in the test group B remained relatively stable compared to those in the other two groups, while the body weight of the dogs in the positive control group decreased, which indicates that the nutritional cream for the aged dogs in the group a had the best growth promoting effect, the nutritional cream for the aged dogs in the group B had the next best growth promoting effect, and the commercial nutritional cream for the red dogs in the positive control group had the worst growth promoting effect.

As can be seen from table 6, the average daily gain weight of the dogs in test group a and test group B increased over the test period, but the body weight of the positive control group at the end of the test decreased rather than the initial body weight. The average daily gain of the dogs in the test group A is remarkably higher than that of the dogs in the positive control group (P <0.05), while the average daily gain of the dogs in the test group B is also increased relative to the positive control group, but is not remarkable, which indicates that the A1 senile nutrition cream can remarkably increase the average daily gain of the test dogs compared with the commercial red dog nutrition cream, and the B1 senile nutrition cream can also increase the average daily gain of the dogs to a certain extent, but has an increased trend but does not reach a remarkable level relative to the commercial red dog nutrition cream.

TABLE 5 weight record chart of nutrition paste test dog for senior dog

TABLE 6 Effect of nutritional pastes for senior dogs on average daily gain of test dogs

	Test group A	Test group B	Positive control group	P value
					Average daily gain (kg/d)	0.007±0.06a	0.001±0.005ab	-0.017±0.006b	0.027

Note: data are presented as mean ± standard deviation, with differences from the shoulder mark indicating significant differences (P < 0.05).

2. Feed intake

As can be seen from Table 7 and FIG. 3, the three groups of nutrient pastes have no significant difference on the average daily food intake of the test aged dogs, and can almost completely eat the food of 350g of dogs. From the 4 th day of the test, the female dogs in each group can not eat all the dog foods completely, and the residual amount of the dog foods eaten by each dog is not more than 25g, which indicates that the A1 nutritional paste for old dogs, the B1 nutritional paste for old dogs and the commercial red dog nutritional paste can not generate adverse effect on the food consumption.

TABLE 7 influence of nutritional paste for senior dogs on average daily food intake of test dogs

	Test group A	Test group B	Positive control group	P value
					Average daily food intake (g/d)	347.36±1.19	347.17±1.28	348.11±1.89	0.67

Note: data are presented as mean ± standard deviation, with differences from the shoulder mark indicating significant differences (P < 0.05).

3. Case of quilt fur

As shown in table 8 and fig. 4 to 6, the fur status of the three groups of test dogs was overall good during the test period, and all dogs in each group exhibited slight depilation from day 1 of the test without significant difference between the groups. In fact, beagle dogs had normal seasonal alopecia in spring, regardless of the test article. Dehairing occurred in all dogs of each group on test days 1, 5, 10, 15, 20. The depilation phenomenon was relieved in the later period of the test, on the 25 th day of the test, the depilation phenomenon of 4 dogs disappeared in the test group a, the depilation phenomenon of 2 dogs remained slight, the depilation phenomenon of 5 dogs disappeared in the test group B, and the depilation phenomenon of 1 dog remained slight, whereas the depilation phenomenon of 1 dog remained in the positive control group and the depilation phenomenon of 2 dogs remained slight. At day 30 of the test, the dehairing phenomenon disappeared in all dogs of test group a, only 1 had remained slightly unhairing in test group B, 1 remained slightly unhairing in the positive control group, and all dogs were shinned more than in the initial period of the test. The A1 nutritional paste for the aged dogs and the B1 nutritional paste for the aged dogs do not have adverse effects on the hair state of test dogs, and the hair loss preventing effects of the A1 nutritional paste for the aged dogs and the B1 nutritional paste for the aged dogs are relatively better than those of the commercial red dog nutritional paste from the recovery condition of the later hair loss phenomenon.

TABLE 8 Effect of nutritional cream for senior dogs on the state of dog hair

4. Skin condition

As shown in table 9, the skin condition of the three groups of test geriatric dogs was overall good, with a slight dehairing on the backs of all dogs in each group from day 1 of the test, with no significant difference between groups. The depilation was relieved at the later stage of the test. In the whole test period, the skin of all dogs has no abnormal conditions such as dander, red rash, red swelling and the like. The results show that neither the A1 senile nutrition cream nor the B1 senile nutrition cream can generate adverse effects on the skin state of the test dog, and the overall effects on the skin are equivalent to the overall effects on the skin of the commercially available C1 red dog control nutrition cream.

TABLE 9 Effect of nutritional cream for senior dogs on skin Condition of test dogs

5. Fecal status

As shown in table 10, during the test period, the three groups of test aged dogs mostly had yellow soft stools and a few yellow brown hard stools, and both stools were normal and had no diarrhea or other abnormal conditions. The three nutrient pastes do not cause the digestion problems of diarrhea and the like of the test dog.

TABLE 10 Effect of nutrition cream for senior dogs on fecal status of test dogs (number of dogs)

6. Mental state

As shown in table 11, the mental states of three groups of test aged dogs were very active throughout the test period. When the testers pass, all the three groups of test dogs have the performance of barking, tail swaying, climbing, front paw stretching and the like, the mental state score reaches 7 points, and all the dogs have no phenomenon of listlessness or lying down. The results show that neither the A1 nutrition paste for senior dogs nor the B1 nutrition paste for senior dogs adversely affects the mental state of the test dogs.

TABLE 11 Effect of nutritional cream for senior dogs on mental status of test dogs

In summary, the A1 nutrient paste for senior dogs and the B1 nutrient paste for senior dogs serve as nutrient supplements for senior dogs, and have remarkable food calling and weight gaining effects on test dogs, wherein the food calling effect of the A1 nutrient paste for senior dogs is superior to that of the B1 nutrient paste for senior dogs, and the A1 nutrient paste and the B1 nutrient paste have positive effects on the hair state of the test senior dogs and have no adverse effect on food consumption, skin state, fecal state, mental state and the like.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit of the corresponding technical solutions of the embodiments of the present invention.