阅读说明：本技术 一种高效细胞孔板蛋白刮出器 (High-efficient cell orifice plate albumen is scraped out ware ) 是由 牛茜 马万里 叶红 王猛 梁丽梅 程培培 陈帅君 冯晓 周娅娅 蒋曳涵 李倩 于 2021-10-14 设计创作，主要内容包括：本发明涉及一种高效细胞孔板蛋白刮出器,包括手柄、传动机构和多个刮除机构,所述手柄与所述传动机构连接,多个所述刮除机构以所述传动机构为中心对称排列,并与所述传动机构通过横向连接轴连接,所述手柄内设有电机和与所述电机连接的传动轴,所述传动轴的顶端与所述传动机构可拆卸地连接,所述手柄上设有控制所述电机转速的调速按钮。本发明可以一次性刮取多个细胞孔板内的蛋白,不仅保证刮除干净,减少蛋白残留,且大大提高刮除效率,便于实验操作。(The invention relates to a high-efficiency cell pore plate protein scraper which comprises a handle, a transmission mechanism and a plurality of scraping mechanisms, wherein the handle is connected with the transmission mechanism, the scraping mechanisms are symmetrically arranged by taking the transmission mechanism as a center and are connected with the transmission mechanism through a transverse connecting shaft, a motor and a transmission shaft connected with the motor are arranged in the handle, the top end of the transmission shaft is detachably connected with the transmission mechanism, and a speed regulating button for controlling the rotating speed of the motor is arranged on the handle. The invention can scrape protein in a plurality of cell pore plates at one time, thereby ensuring clean scraping, reducing protein residue, greatly improving scraping efficiency and facilitating experimental operation.)

1. The utility model provides a high-efficient cell orifice plate protein scraper which characterized in that: including handle, drive mechanism and a plurality of scraping mechanism, the handle with drive mechanism connects, and is a plurality of scrape the mechanism with drive mechanism is central symmetrical arrangement, and with drive mechanism passes through the transverse connection hub connection, be equipped with in the handle motor and with the transmission shaft that the motor is connected, the top of transmission shaft with drive mechanism detachably connects, be equipped with control on the handle the speed governing button of motor speed, be equipped with in the handle with battery and controller that the motor electricity is connected.

2. The high-efficiency cell well plate protein scraper as claimed in claim 1, wherein: the transmission mechanism comprises a transmission box and a longitudinal connecting shaft, the longitudinal connecting shaft is detachably connected with the transmission shaft, a bevel gear is arranged at the top end of the longitudinal connecting shaft, transverse bevel gears matched with the bevel gears are arranged at two ends of the transverse connecting shaft, the bevel gears are matched with the bevel gears and arranged in the transmission box, the scraping mechanism comprises a scraping box and a scraping rod, the scraping rod is provided with longitudinal bevel gears matched with the transverse bevel gears, the longitudinal bevel gears are matched with the longitudinal bevel gears and arranged in the scraping box, and the top end of the scraping rod is connected with a scraper.

3. The high-efficiency cell well plate protein scraper as claimed in claim 2, wherein: the longitudinal connecting shaft is provided with an external spline, the top end of the transmission shaft is provided with an internal spline matched with the external spline, and the handle is provided with a locking screw.

4. The high-efficiency cell well plate protein scraper as claimed in claim 2, wherein: the scraper comprises a scraper body connected with the scraping rod, and an elastic blade is arranged on the outer side edge of the scraper body.

5. The high-efficiency scraper for cell well plate protein according to claim 1 or 2, wherein: the transverse connecting shaft is adjustable and telescopic.

6. The high-efficiency cell well plate protein scraper as claimed in claim 1, wherein: the number of the scraping mechanisms is 6 or 12.

7. The high-efficiency cell well plate protein scraper as claimed in claim 1, wherein: the number of the speed regulating buttons is 3, and the speed regulating buttons respectively control the three-gear rotating speed of the motor.

8. The high-efficiency cell well plate protein scraper as claimed in claim 4, wherein: the elastic blade is made of Polytetrafluoroethylene (PTFE).

9. The high-efficiency cell well plate protein scraper as claimed in claim 5, wherein: the transverse connecting shaft comprises an extending section and an inserting section, wherein a transmission key is arranged on the extending section, an inserting groove for the extending section to insert is formed in the inserting section, and a key groove and an elastic washer are arranged in the inserting groove.

The technical field is as follows:

the invention relates to the technical field of biological experiment instruments, in particular to a high-efficiency cell pore plate protein scraper.

Background art:

cell or protein harvesting in culture is a fundamental operation in cell biology, which involves taking adherently growing cells in culture and producing proteins for subsequent experimental manipulation or cryopreservation. At present, the method commonly used for obtaining the protein from the culture vessel is to use a cell scraper to scrape the adherent protein off for obtaining. Present typical cell scraper is for scraping the cutter body and scrape the cutter arbor and constitute, when scraping and getting the albumen, need stretch into the cell orifice plate of culture with the scraper and scrape several times and get, because scrape the scraper head fixed with scraper arbor angle, thereby unable timely angle regulation adapts to the operation demand, can't contact all surfaces that cell orifice plate albumen grows when leading to the harvest albumen, leaves the dead angle, is unfavorable for drawing of albumen, prolongs operating time, slows down the experiment process, reduces and acquires efficiency. Patent No. ZL201921155566.6 discloses an automatic cell scraper, which comprises a handle, a connecting rod, a scraper seat, a scraper blade, a motor, a controller and a battery, wherein one end of the connecting rod is inserted into the handle, the other end of the connecting rod is connected with a connecting part, and a rotating shaft penetrates through one end of the connecting part, which is far away from the connecting rod, and is rotatably connected with the rotating shaft; one end of the rotating shaft is fixedly connected with the scraper seat, the other end of the rotating shaft extends out of the scraper seat and is in transmission connection with the output end of a motor, and the motor is fixed on the scraper seat; the scraper blade is fixed at the bottom end of the scraper seat; the battery and the controller are arranged in the handle, and the motor and the battery are electrically connected with the controller. The automatic cell scraper is simple in structure and convenient to use, the angle of the scraper blade can be adjusted according to actual requirements, and the cell or protein obtaining efficiency is improved. However, because six-hole or twelve-hole plates are generally adopted for one-time culture of multiple parts in the cell hole plate, the existing automatic cell scraping only can scrape the protein in one cell hole plate once, so that the scraping is easy, the protein is easy to remain in the hole plate, the efficiency is low, and the subsequent experimental operation is influenced.

The invention content is as follows:

technical problem to be solved

The invention aims to provide an efficient cell pore plate protein scraper which is used for scraping protein in a cell pore plate and solves the problems that the protein in the cell pore plate can only be scraped once in the prior art, the scraping is easy to be incomplete, the protein is easy to remain in the pore plate, the efficiency is low, and the subsequent experiment operation is influenced.

The technical scheme is as follows:

in order to solve the technical problems, the invention adopts the following technical scheme: the utility model provides a ware is scraped to high-efficient cell orifice plate albumen, includes handle, drive mechanism and a plurality of scraping mechanism, the handle with drive mechanism connects, and is a plurality of scraping mechanism with drive mechanism is central symmetrical arrangement, and with drive mechanism passes through the transverse connection hub connection, be equipped with in the handle motor and with the transmission shaft that the motor is connected, the top of transmission shaft with drive mechanism detachably connects, be equipped with control on the handle the speed governing button of motor speed, be equipped with in the handle with battery and controller that the motor electricity is connected.

The transmission shaft arranged in the handle transmits the power of the motor to the transmission mechanism, the transmission mechanism is simultaneously connected with a plurality of scraping mechanisms, the arrangement pattern of the scraping mechanisms is consistent with that of culture holes on a cell pore plate, each scraping mechanism corresponds to one culture hole on the cell pore plate, the scraping mechanism is inserted into the culture hole, and the protein in the culture hole is scraped out under the driving of the motor, can scrape the protein in a plurality of cell pore plates at one time, not only ensures the scraping to be clean and reduces the protein residue, the scraping efficiency is greatly improved, the experimental operation is convenient, the handle and the transmission mechanism can be disassembled, the transmission mechanism and the scraping mechanism with different specifications can be conveniently replaced, the scraping mechanism can be used for scraping proteins in cell pore plates with different quantities and specifications by changing the number of the scraping mechanisms connected with the transmission mechanism.

Further, drive mechanism includes transmission box and longitudinal connecting axle, the longitudinal connecting axle with transmission shaft detachably connects, the top of longitudinal connecting axle is equipped with the bevel gear, the both ends of transverse connecting axle all be equipped with bevel gear complex transverse bevel gear, the bevel gear with bevel gear complex transverse bevel gear sets up in the transmission box, strike off the mechanism including striking off the box and striking off the pole, strike off be equipped with on the pole with transverse bevel gear complex longitudinal bevel gear, longitudinal bevel gear with longitudinal bevel gear complex transverse bevel gear sets up strike off in the box, strike off the top of pole and be connected with the scraper. The longitudinal connecting shaft is used for being connected with the transmission shaft, the motor rotates to drive the transmission shaft and the longitudinal connecting shaft to rotate, the bevel gears drive the transverse bevel gears to rotate at the same time, power is transmitted to the scrapers from the transverse bevel gears through the transverse connecting shaft, the longitudinal bevel gears and the scraping rod, one motor drives the plurality of scrapers to rotate at the same time, and therefore protein in the cell pore plates can be scraped at one time.

Furthermore, the longitudinal connecting shaft is provided with an external spline, the top end of the transmission shaft is provided with an internal spline matched with the external spline, and the handle is provided with a locking screw. The longitudinal connecting axle with the transmission shaft passes through the external splines with the cooperation of internal spline is convenient for transmit power when conveniently dismantling the connection, it is right that locking screw is used for connecting the back the external spline with the cooperation department of internal spline locks, prevents to take place longitudinal sliding.

Further, the scraper comprises a scraper body connected with the scraping rod, and the outer edge of the scraper body is provided with an elastic blade. The cell culture hole scraper is characterized in that the scraper body is inserted into a culture hole in a cell hole plate, the elastic blade is tightly attached to and contacted with the bottom and the peripheral edge of the culture hole, the scraper rod rotates to drive the scraper body to rotate in the culture hole, and the elastic blade thoroughly scrapes out protein at the bottom and the peripheral edge of the culture hole, so that the protein is thoroughly scraped out, and residues are reduced.

Furthermore, the transverse connecting shaft is adjustable and telescopic, so that the distance between each scraping mechanism and the transmission mechanism can be conveniently adjusted to adapt to different cell pore plate specifications and sizes.

Further, the scraping mechanism has 6 or 12 scraping mechanisms, and is suitable for 6-hole or 12-hole standard-size cell hole plates.

Furthermore, the number of the speed regulating buttons is 3, and the three-gear rotating speed of the motor is respectively controlled, so that the scraper is controlled to scrape protein at different rotating speeds according to requirements.

Further, the elastic blade is made of Polytetrafluoroethylene (PTFE). Polytetrafluoroethylene (PTFE) has good elasticity and is difficult to adhere to protein, so that the scraping is ensured to be clean, and the protein loss is avoided.

Furthermore, the transverse connecting shaft comprises an extending section and an inserting section, wherein a transmission key is arranged on the extending section, an inserting groove for the extending section to insert is formed in the inserting section, and a key groove and an elastic washer are arranged in the inserting groove. The length of the transverse connecting shaft inserted into the insertion groove is adjusted by adjusting the length of the extension section, the transmission key is inserted into the key groove to realize the transmission function, and the elastic washer is used for increasing the friction force between the extension section and the insertion groove and preventing axial sliding.

(III) the beneficial effects are as follows:

compared with the prior art, the invention has the following beneficial effects: through setting up the handle, drive mechanism and a plurality of scraping mechanism, the handle is connected with drive mechanism, a plurality of scraping mechanism pass through transverse connection hub connection with drive mechanism, be equipped with the motor in the handle and the transmission shaft of being connected with the motor, the top and the drive mechanism detachably of transmission shaft are connected, set up the speed regulating button of control motor rotational speed on the handle, the range pattern of a plurality of scraping mechanism is unanimous with the range pattern of cultivateing the hole on the cell orifice plate, every scraping mechanism corresponds a cultivation hole on the cell orifice plate, can select suitable scraping mechanism according to the quantity of cultivateing the hole on the cell orifice plate, and once only scrape the albumen of getting in a plurality of cell orifice plates, not only guarantee to scrape totally, reduce albumen residue, and improve greatly and scrape out efficiency, the experimental operation of being convenient for.

Description of the drawings:

in order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings required to be used in the embodiments will be briefly described below.

FIG. 1 is a schematic diagram of the overall structure of a high-efficiency cell-well plate protein scraper according to an embodiment of the present invention;

FIG. 2 is a top view of a high efficiency cell well plate protein scraper according to an embodiment of the present invention;

FIG. 3 is a cross-sectional view of a high-efficiency cell-well-plate protein scraper according to an embodiment of the present invention

FIG. 4 is a cross-sectional view of the handle portion of the high-efficiency cell well plate protein scraper according to the embodiment of the invention;

FIG. 5 is a cross-sectional view of the driving mechanism and the scraping mechanism of the high-efficiency cell well plate protein scraper according to the embodiment of the invention;

in the figure: 1. a handle; 2. a transmission mechanism; 21. a transmission box 22 and a longitudinal connecting shaft; 23. a bevel gear; 24. a transverse bevel gear; 3. a scraping mechanism; 31. scraping the box; 32. a scraping bar; 33. a longitudinal bevel gear; 34. a scraper; 341. a cutter body; 342. an elastic blade; 4. a transverse connecting shaft; 41. a protruding section; 42. an insertion section; 43. a drive key; 44. inserting the groove; 45. a keyway; 46. an elastic washer; 5. a motor; 6. a drive shaft; 7. a speed regulating button; 8. a battery; 9. a controller; 10. an external spline; 11. an internal spline; 12. locking screw

The specific implementation mode is as follows:

the technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.

As shown in fig. 1, fig. 2, fig. 3, fig. 4 and fig. 5, the high-efficiency scraping device for cell well plate protein comprises a handle 1, a transmission mechanism 2 and a plurality of scraping mechanisms 3, wherein the handle 1 is connected with the transmission mechanism 2, the plurality of scraping mechanisms 3 are symmetrically arranged by taking the transmission mechanism 2 as a center and are connected with the transmission mechanism 2 through a transverse connecting shaft 4, a motor 5 and a transmission shaft 6 connected with the motor 5 are arranged in the handle 1, the top end of the transmission shaft 6 is detachably connected with the transmission mechanism 2, a speed regulating button 7 for controlling the rotating speed of the motor 5 is arranged on the handle 1, and a battery 8 and a controller 9 which are electrically connected with the motor 5 are arranged in the handle 1.

Preferably, the transmission mechanism 2 comprises a transmission box 21 and a longitudinal connection shaft 22, the longitudinal connection shaft 22 is detachably connected with the transmission shaft 6, a bevel gear 23 is arranged at the top end of the longitudinal connection shaft 22, transverse bevel gears 24 matched with the bevel gears 23 are arranged at both ends of the transverse connection shaft 4, the bevel gears 23 and the transverse bevel gears 24 matched with the bevel gears 23 are arranged in the transmission box 21, the scraping mechanism 3 comprises a scraping box 31 and a scraping rod 32, a longitudinal bevel gear 33 matched with the transverse bevel gear 24 is arranged on the scraping rod 32, the longitudinal bevel gear 33 and the transverse bevel gear 24 matched with the longitudinal bevel gear 33 are arranged in the scraping box 31, and a scraper 34 is connected to the top end of the scraping rod 32. The longitudinal connecting shaft 22 is used for being connected with the transmission shaft 6, the motor 5 rotates to drive the transmission shaft 6 and the longitudinal connecting shaft 22 to rotate, the bevel gears 23 drive the transverse bevel gears 24 to rotate at the same time, power is transmitted to the scrapers 34 from the transverse bevel gears 24 through the transverse connecting shaft 4, the longitudinal bevel gears 33 and the scraping rod 32, the motor 5 drives the scrapers 34 to rotate at the same time, and therefore protein in a plurality of cell pore plates can be scraped at one time.

Preferably, the longitudinal connecting shaft 22 is provided with an external spline 10, the top end of the transmission shaft 6 is provided with an internal spline 11 matched with the external spline 10, and the handle 1 is provided with a locking screw 12. The longitudinal connecting shaft 22 and the transmission shaft 6 are matched through the external spline 10 and the internal spline 11, so that the detachable connection is facilitated, meanwhile, the power transmission is facilitated, and the locking screw 12 is used for locking the matched part of the external spline 10 and the internal spline 11 after the connection, so that the longitudinal sliding is prevented.

Preferably, the scraper 34 includes a cutter body 341 connected to the scraper bar 32, and the outer side edge of the cutter body 341 is provided with an elastic blade 342. The cutter body 341 is used for inserting into the culture hole on the cell pore plate, the elastic blade 342 is used for closely attaching and contacting with the bottom and the peripheral edge of the culture hole, the cutter body 341 is driven to rotate in the culture hole by the rotation of the scraping rod 32, and the elastic blade 342 thoroughly scrapes out the protein at the bottom and the peripheral edge of the culture hole, thereby ensuring that the protein is thoroughly scraped out and reducing the residue.

Preferably, the transverse connecting shaft 4 is adjustable and telescopic, so that the distance between each scraping mechanism 3 and the transmission mechanism 2 can be adjusted conveniently to adapt to different cell pore plate specifications.

Preferably, the scraping mechanism 3 has 6 or 12, and is suitable for 6-well or 12-well standard-size cell well plates.

Preferably, there are 3 speed-adjusting buttons 7, each of which controls the third gear of the motor 5 to control the scraper 34 to scrape out protein at different speeds according to the requirement.

Preferably, the elastic blade 342 is made of Polytetrafluoroethylene (PTFE). Polytetrafluoroethylene (PTFE) has good elasticity and is difficult to adhere to protein, so that the scraping is ensured to be clean, and the protein loss is avoided.

Preferably, the transverse connecting shaft 4 includes an extending section 41 and an inserting section 42, the extending section 41 is provided with a transmission key 43, the inserting section 42 is provided with an inserting groove 44 for inserting the extending section 41, and the inserting groove 44 is provided with a key groove 45 and an elastic washer 46. The length of the transverse connecting shaft 4 is adjusted by adjusting the length of the extension section 41 in the insertion groove 44, the transmission key 43 is inserted in the key groove 45 to realize the transmission function, and the elastic washer 46 is used for increasing the friction force between the extension section 41 and the insertion groove 44 and preventing the axial sliding.

The use method of the high-efficiency cell pore plate protein scraper comprises the following steps:

according to the number of the cell pore plates, selecting a transmission mechanism 2 with 6 or 12 scraping mechanisms 3 to be matched with a handle 1, inserting an external spline 10 on a longitudinal connecting shaft 22 into an internal spline 11 on the transmission shaft 6, locking by using a locking screw 12, then inserting a scraper 34 on the scraping mechanisms 3 into a culture hole on the cell pore plate to enable the edge of an elastic blade 342 to be tightly attached to the bottom and the peripheral edge of the culture hole, then pressing a speed regulating button 7 on the handle 1 to start a motor 5, transmitting the power of the motor 5 to the transmission mechanism 2 by the transmission shaft 6, simultaneously connecting the transmission mechanism 2 with a plurality of scraping mechanisms 3, enabling the arrangement pattern of the plurality of scraping mechanisms 3 to be consistent with the arrangement pattern of the culture holes on the cell pore plate, enabling each scraping mechanism 3 to correspond to one culture hole on the cell pore plate, transmitting the power output by the motor 5 to a scraper 34 by the transmission of the transmission mechanism 2, scraper 34 rotates certain angle and scrapes out the albumen in will cultivateing the downthehole, can once only scrape the albumen of getting in a plurality of cell orifice plates, it is clean not only to guarantee to scrape out, it is remained to reduce the albumen, and improve greatly and scrape out efficiency, the experimental operation of being convenient for, handle 1 can be dismantled with drive mechanism 2, the drive mechanism 2 and the scraping mechanism 3 of different specifications of being convenient for change, through the quantity of scraping mechanism 3 that changes and be connected with drive mechanism 2, can be applicable to and scrape out the albumen in the cell orifice plate of different quantity specifications.

In conclusion, the high-efficiency scraper for cell pore plate proteins provided by the invention is used for scraping proteins in a cell pore plate, and solves the problems that only one cell pore plate protein can be scraped at one time in the prior art, the scraping is easy to be incomplete, the proteins are easy to remain in the pore plate, the efficiency is low, and the subsequent experimental operation is influenced.

The present invention has been described above by way of example, but the present invention is not limited to the above-described specific embodiments, and any modification or variation made based on the present invention is within the scope of the present invention as claimed.