阅读说明：本技术 一种无毒无刺激环境消毒剂及其制备方法 (Non-toxic non-irritant environment disinfectant and preparation method thereof ) 是由 汪渡舟 季汉彬 汪星任 赵欣 汪星全 于 2021-09-09 设计创作，主要内容包括：本发明涉及一种无毒无刺激环境消毒剂及其制备方法,配方包括：三氯异氰尿酸、溴化合物、碱化合物、磷酸钠、硅酸盐、硫酸钠、表面活性剂。制备方法包括以下步骤：1)精准称量；2)混合；3)加入表面活性剂。本发明优点在于：配比合理,制作简单,能够有效防止疫源地的病毒扩散传播,有效切断传染源,且无毒无刺激,广泛应用于医院、小区、街道、室内、车内、卫生间、储藏室等的消毒工作。具有很强的实用性和市场推广价值。(The invention relates to a non-toxic non-irritant environment disinfectant and a preparation method thereof, and the formula comprises: trichloroisocyanuric acid, a bromine compound, an alkali compound, sodium phosphate, silicate, sodium sulfate and a surfactant. The preparation method comprises the following steps: 1) accurately weighing; 2) mixing; 3) adding a surfactant. The invention has the advantages that: the disinfectant is reasonable in proportion and simple to manufacture, can effectively prevent virus from spreading and spreading in epidemic focus places and effectively cut off infectious sources, is non-toxic and non-irritant, and is widely applied to disinfection work of hospitals, communities, streets, indoor places, vehicles, toilets, storerooms and the like. Has strong practicability and market popularization value.)

1. A non-toxic and non-irritant environmental disinfectant is characterized by comprising the following components in percentage by mass: 10-40% of trichloroisocyanuric acid, 15-25% of a bromine compound, 10-20% of an alkali compound, 8-15% of sodium phosphate, 5-10% of silicate, 15-25% of sodium sulfate and 6-12% of a surfactant.

2. The preparation method of the nontoxic non-irritant environmental disinfectant is characterized by comprising the following steps:

1) and (3) accurate weighing: accurately weighing the required materials by using an electronic balance;

2) mixing: mixing the weighed trichloroisocyanuric acid, bromine compound, alkali compound, sodium phosphate, silicate and sodium sulfate, and stirring at the speed of 15-50 rpm until the trichloroisocyanuric acid, the bromine compound, the alkali compound, the sodium phosphate, the silicate and the sodium sulfate are completely and uniformly mixed to obtain white powder;

3) adding a surfactant: adding surfactant into the initial white powder to obtain the final disinfectant.

Technical Field

The invention relates to the technical field of disinfection, in particular to a non-toxic non-irritant environment disinfectant and a preparation method thereof.

Background

Disinfection refers to the treatment of killing or eliminating pathogenic microorganisms on the transmission medium to make the transmission medium harmless. The disinfection work is good, the occurrence of infectious diseases and various infections is necessarily reduced, the disinfection not only has important practical significance for preventing diseases in hospitals and society, but also is not exceptional for families and individuals, and disinfection measures should be implemented to thousands of households so as to fully play the role of the disinfection work in preventing diseases. The existing disinfection methods can be divided into physical disinfection methods and chemical disinfection methods, and methods for disinfection treatment by using physical factors such as ultraviolet irradiation, incineration, cooking and the like belong to the physical disinfection methods, and are mainly used for disinfection of articles, but the cost for purchasing and using equipment is higher; the method of using chemical articles such as alcohol, iodine liquor, potassium permanganate, bleaching powder and benzalkonium bromide to make disinfection treatment belongs to the field of chemical disinfection method, and its application range is extensive, but they have the actions of corroding and bleaching disinfected articles, in particular, they have a certain irritation or toxicity for human body.

While disinfecting the environment, the irritation to the human body is not negligible. The skin, mucous membrane and other fragile parts of the human body are often damaged and eroded by toxicity under the stimulation of the traditional disinfectant.

Therefore, a nontoxic and non-irritant environmental disinfectant is in urgent need of research.

Disclosure of Invention

The invention aims to provide a non-toxic and non-irritant environment disinfectant and a preparation method thereof, so as to solve the problems in the background technology.

In order to solve the technical problems, the technical scheme provided by the invention is as follows: a non-toxic and non-irritant environmental disinfectant comprises the following components in percentage by mass: 10-40% of trichloroisocyanuric acid, 15-25% of a bromine compound, 10-20% of an alkali compound, 8-15% of calcium phosphate, 5-10% of silicate, 15-25% of sodium sulfate and 6-12% of a surfactant.

The preparation method of the nontoxic non-irritant environmental disinfectant specifically comprises the following steps:

1) and (3) accurate weighing: accurately weighing the required materials by using an electronic balance;

2) mixing: mixing the weighed trichloroisocyanuric acid, bromine compound, alkali compound, sodium phosphate, silicate and sodium sulfate, and stirring at the speed of 15-50 rpm until the trichloroisocyanuric acid, the bromine compound, the alkali compound, the sodium phosphate, the silicate and the sodium sulfate are completely and uniformly mixed to obtain white powder;

3) adding a surfactant: adding surfactant into the initial white powder to obtain the final disinfectant.

The invention has the advantages that: the disinfectant is reasonable in proportion and simple to manufacture, can effectively prevent virus from spreading and spreading in epidemic focus places and effectively cut off infectious sources, is non-toxic and non-irritant, and is widely applied to disinfection work of hospitals, communities, streets, indoor places, vehicles, toilets, storerooms and the like. Has strong practicability and market popularization value.

Detailed Description

The invention is illustrated below by means of specific examples, without being restricted thereto.

Example 1

A non-toxic and non-irritant environmental disinfectant comprises the following components in percentage by mass: 21% of trichloroisocyanuric acid, 25% of a bromine compound, 20% of a base compound, 8% of calcium phosphate, 5% of silicate, 15% of sodium sulfate and 6% of a surfactant.

The preparation method of the nontoxic non-irritant environmental disinfectant specifically comprises the following steps:

1) and (3) accurate weighing: accurately weighing the required materials by using an electronic balance;

2) mixing: mixing the weighed trichloroisocyanuric acid, bromine compound, alkali compound, sodium phosphate, silicate and sodium sulfate, and stirring at the speed of 15-50 rpm until the trichloroisocyanuric acid, the bromine compound, the alkali compound, the sodium phosphate, the silicate and the sodium sulfate are completely and uniformly mixed to obtain white powder;

3) adding a surfactant: adding surfactant into the initial white powder to obtain the final disinfectant.

Example 2

A non-toxic and non-irritant environmental disinfectant comprises the following components in percentage by mass: 10% of trichloroisocyanuric acid, 15% of a bromine compound, 15% of an alkali compound, 15% of sodium phosphate, 10% of silicate, 25% of sodium sulfate and 10% of a surfactant.

A preparation method of a nontoxic non-irritant environmental disinfectant specifically comprises the following steps:

1) and (3) accurate weighing: accurately weighing the required materials by using an electronic balance;

2) mixing: mixing the weighed trichloroisocyanuric acid, bromine compound, alkali compound, calcium phosphate, silicate and sodium sulfate, and stirring at the speed of 15-50 rpm until the three are completely and uniformly mixed to obtain a white powder finished product;

3) adding a surfactant: adding surfactant into the initial white powder to obtain the final disinfectant.

Example 3

A non-toxic and non-irritant environmental disinfectant comprises the following components in percentage by mass: 37% of trichloroisocyanuric acid, 18% of a bromine compound, 10% of an alkali compound, 8% of sodium phosphate, 5% of silicate, 15% of sodium sulfate and 7% of a surfactant.

The preparation method of the nontoxic non-irritant environmental disinfectant specifically comprises the following steps:

1) and (3) accurate weighing: accurately weighing the required materials by using an electronic balance;

2) mixing: mixing the weighed trichloroisocyanuric acid, bromine compound, alkali compound, sodium phosphate, silicate and sodium sulfate, and stirring at the speed of 15-50 rpm until the trichloroisocyanuric acid, the bromine compound, the alkali compound, the sodium phosphate, the silicate and the sodium sulfate are completely and uniformly mixed to obtain white powder;

3) adding a surfactant: adding surfactant into the initial white powder to obtain the final disinfectant.

When the invention is implemented, the invention is mainly used for the environmental disinfection of the virus, preventing the spread and the transmission of the virus in the epidemic focus and effectively cutting off the transmission source. In the disinfection process, the stimulation and harm to human bodies caused by the disinfected environment are reduced and avoided. And the disinfectant is nontoxic and non-irritant, and can be widely applied to disinfection work of hospitals, communities, streets, rooms, vehicles, toilets, storerooms and the like. Has good popularization prospect and market value.

Detection test

Acute oral toxicity test (mouse)

Materials and animals

1. The tested sample is 'Xunjele' disinfectant, batch No. 20140105001, white powder, and samples 1000, 2150, 4640, 10000mg and purified water are respectively weighed to 20, 40ml to be used as the tested substance.

2. Animal, 20 clean grade healthy ICR mice, weight of 18.0-22.0g, half male and female, provided by the experimental animal center of Nanjing medical university, and production license number SCXK (Su) 2013-. Animal feed source and identification No. Suzhou Shuangshi laboratory animal feed science and technology Limited, Su E Fei Sheng (2002) 006.

3. The test conditions are that the SPF level experimental animal environment uses the license number SYXK (Su) 2012-0037; the environmental temperature is 22+ 2' C, and the relative humidity is 40-70%. The main instruments are an electronic scale (number 05-705) and an electronic balance (number 05-314).

Second, method

1. The test is carried out according to the Disinfection technical Specification of the Ministry of health (2002.11), the second part, Disinfection product test technical Specification, and 2.3.1 acute oral toxicity test.

2. The test method is a Hohner method. The designed dose is 1000, 2150, 4640 and 10000mg/kg b.w, after the mice are fasted overnight, the high dose is administrated by gavage twice at an interval of 2h, the rest groups are administrated by gavage once through mouth, and the gavage volume is 20ml/kg b.wt. The food is fed 4h after the gavage, and the observation period is 14 d.

Third, test results

After the stomach is perfused, the poisoning manifestations of prone position, weakness, dyspnea and the like according to the dosage are seen about 10min after the mice are perfused, the death occurs within 1-24h, the mice are induced by flatulence, the gastric mucosa is whitish, and the 2 nd day of the non-dead animals is gradually recovered. The results of the test animal deaths are shown in the following table:

test result of acute oral toxicity of mice

Fourth, conclusion

The acute oral LDso values (95% confidence limit) of the samples for male and female mice are 5010 (3440-.

One complete skin irritation test

Materials and animals

1. The test sample is white powder with the highest available chlorine content of 2000mg/L, and the preparation process includes adding 15g of water into each sample to prepare solution with available chlorine content of 200mg/L, adding 15g of pure water to 300ml to prepare 5 times the solution with the highest available chlorine content as the test matter.

2. Animals including 3 healthy New Zealand white rabbits without skin diseases, provided by a general grade of Jinling breeding rabbit farm, with the qualification number of SCXK (Su) 2012-0003 and the weight of 2.4-2.7 kg.

3. The test conditions are that the animal environment facility qualification number is SYXK (Su) No. 2012 and 0036; ambient temperature 22 s 2 "C; relative humidity of 40-70%; the source and the certificate of the feed are Jiangsu province cooperative medicine bioengineering limited company, Su A Fei Sheng Zi (2002) 009. The main instrument is a baby scale (05-323).

Second, method

1. The test was based on the skin irritation test of the second part 2.3.3 of the Ministry of health (2002 edition) Disinfection Specification.

2. The test method comprises shearing hair on both sides of spinal column for 24h before animal experiment to prepare skin 3cmX3cm, dripping 0.5ml of test substance on one side of 2.5cmX2.5cm, removing hair, covering with 2 layers of gauze and 1 layer of oilpaper, fixing with non-irritant adhesive plaster, applying the other side as blank control, washing off residual test substance with warm water after 4h, and observing and recording skin reaction after 1, 24, and 48 h.

Third, test results

The results of the tests are given in the following table:

test substance one-time whole skin irritation response scoring for rabbits

Fourth, conclusion

The highest integral mean value of the 5-time highest application concentration solution of the sample on the primary intact skin irritation reaction of the rabbits is 0, and the irritation strength is nonirritating.

Primary damaged skin irritation test

Materials and animals

1. The test sample is white powder with the highest available chlorine content of 2000mg/L, and the preparation process includes adding 15g of water into each sample to prepare solution with available chlorine content of 200mg/L, adding 15g of pure water to 300ml to prepare 5 times the solution with the highest available chlorine content as the test matter.

2. Animals including 3 healthy New Zealand white rabbits without skin diseases, provided by a general grade of Jinling breeding rabbit farm, with the qualification number of SCXK (Su) 2012-0003 and the weight of 2.3-2.7 kg.

3. The test conditions are that the animal environment facility qualification number is SYXK (Su) No. 2012 and 0036; the environmental temperature is 22 ℃ and 2 ℃; relative humidity of 40-70%; the source and the certificate of the feed are Jiangsu province cooperative medicine bioengineering limited company, Su A Fei Sheng Zi (2002) 009. The main instrument is a baby scale (05-323).

Second, method

1, the test is based on the skin irritation test of the second part 2.3.3 of the Ministry of health (2002 edition) Disinfection Specification.

2. The test method comprises shearing hair and skin at two sides of spinal column about 3cmX3cm respectively 24h before animal experiment, cleaning and sterilizing exposed skin with 75% alcohol on depilated skin of 2.5cm X2.5cm before applying on test object the next day, drawing a well-shaped damaged wound in skin area with sterilized triangular needle after alcohol volatilizes, wherein the damaged skin only reaches epidermis without damaging dermis. Dropping 0.5ml of the test object into the damaged area, covering with two layers of gauze and one layer of oiled paper, fixing with non-irritant adhesive plaster, coating physiological saline on the other side as control, washing off the residual test object with sterile physiological saline after applying for 4h, and observing and recording skin reaction after 1, 24 and 48 h.

Third, test results

The results of the tests are given in the following table:

test agent response to rabbit-minor skin irritation score

Fourth, conclusion

The highest integral mean value of the stimulation response of the solution with the highest application concentration of 5 times to the once damaged skin of the rabbit is 0, and the stimulation intensity is nonirritant.

Multiple vaginal mucosa irritation test

Materials and animals

1. The tested sample is white powder, the highest application concentration is 2000mg/L of available chlorine, the preparation method of the instruction includes that 15g of water is added into each bag of sample to prepare solution containing 200mg/L of available chlorine, 15g of the sample is added with sterilized normal saline to 300ml to prepare solution with 5 times of the highest application concentration as the tested object.

2. Animals including 6 healthy female New Zealand white rabbits of the general grade provided by the Jinling breeding rabbit farm, the qualification number of SCXK (Su) 2012-0003 and the weight of 2.0-2.2 kg.

3. The test conditions are that the animal environment facility qualification number is SYXK (Su) No. 2012-0036; the environmental temperature is 22 ℃ and 2 ℃; relative humidity of 40-70%; the source and the certificate of the feed are Jiangsu province cooperative medicine bioengineering limited company, Su A Fei Sheng Zi (2002) 009. The main instrument is a baby scale (05-324).

Second, method

1. The examination is based on the vaginal mucosa irritation test of the second part 2.3.5 of the Ministry of health (2002 edition) Disinfection technical Specification.

2. The test method comprises dividing the animals into test group and control group, each group comprises 3 animals. The animal is fixed on the back, and the perineum and the vaginal orifice are exposed. Connecting a small-size disinfection catheter with a 2ml syringe, wetting the catheter with a test object, then gently inserting the catheter into an animal vagina (4-5 cm), slowly injecting the test object with 2ml, and drawing out the catheter to finish contamination. Once a day, for 5 consecutive days of exposure, control animals were treated with saline as such. And (3) killing the animals by adopting an air embolism method 24h after the last infection, taking out the complete vagina after laparotomy, longitudinally cutting, and visually observing whether congestion, edema and other manifestations exist or not for reference during pathological material taking. Fixing vagina in 10% formalin for over 24 hr, selecting tissues at two ends and 3 central parts of vagina, making into slices, and performing histopathological examination after HE staining.

Third, test results

The mild edema of the vaginal mucosa of the animals in the control group, and the mild or moderate congestion and edema of the vaginal mucosa of the 3 animals in the test group can be seen through visual observation. Histopathological observations are shown in the table below.

Test substance scoring of multiple vaginal mucosal irritation responses of animals

The vagina mucosa irritation index is equal to the average integral of the test group, and the average integral of the control group is equal to 10.44-2.67-7.77

Fourthly, the conclusion is that the stimulation index of the solution with the highest application concentration of 5 times of the sample on the vaginal mucosa of the rabbits for many times is 7.77, and the stimulation intensity is mild stimulation.

Acute eye irritation test

Materials and animals

1. The test sample is white powder, the maximum application concentration is 2000mg/L of available chlorine, the preparation method of the instruction includes that 15g of water is added into each bag of sample to prepare solution containing 200mg/L of available chlorine, and 15g of sample is added with 300ml of normal saline to prepare solution with 5 times of the maximum application concentration as the test object.

2. Animals, namely 3 healthy New Zealand white rabbits, provided by a common grade Jinling breeding rabbit farm, the qualification number of the rabbit farm is SCXK (Su) 2012-0003, the weight of the rabbit farm is 2.5-2.7kg, eyes are checked 24h before the test, and people without eye diseases are taken for the test.

3. The test conditions are that the animal environment facility qualification number is SYXK (Su) No. 2012-0036; the ambient temperature is 22 seconds 2C; relative humidity of 40-70%; the source and the certificate of the feed are Jiangsu province cooperative medicine bioengineering limited company, Su A Fei Sheng Zi (2002) 009. The main instrument is a baby scale (05-323).

Second, method

1. The test was based on the acute eye irritation test of the second part 2.3.4 of the Disinfection Specification of the Ministry of health (2002).

2. The test method comprises directly placing 0.1ml of the test object into conjunctival sac of one side of rabbit, passively closing eyelid for 4s, washing with normal saline for 30s, and dripping normal saline on the other side of eye as control. Eye irritation responses were recorded at 1h, 24h, 48hv72h observed after eye dropping.

Third, test results

No other toxic effects than irritation were observed, and the results are shown in the following table:

test results of acute eye irritation of test article on rabbits (30sec rinse)

Mean score, i.e. the sum of the 24h, 48h and 72h scores per animal divided by the number of observations 3

Test results of acute eye irritation of test article on rabbits (30sec rinse)

Fourth, conclusion

The stimulation intensity of the solution with the highest application concentration of 5 times of the sample on the acute eye stimulation response of the rabbit is nonirritant.

Skin allergy test

Materials and animals

1. The test sample is white powder with the highest available chlorine content of 2000mg/L, and the preparation process includes adding 15g of water into each sample to prepare solution with available chlorine content of 200mg/L, adding 15g of pure water to 300ml to prepare 5 times the solution with the highest available chlorine content as the test matter.

2. The animals are 32 healthy white guinea pigs with the weight of 200-.

3. The test conditions are that the animal environment facility qualification number is SYXK (Su) No. 2012 and 0036; the environmental temperature is 22 ℃ soil 2 ℃; relative humidity of 40-70%.

Second, method

1. The test was carried out according to 2.3.6 skin allergy test, second part of the Disinfection Specification, Ministry of health (2002).

2. The test method is a local seal coating (BT) method. Animals were randomly divided into experimental groups and negative control groups of 16 animals each. The back of the skin is unhaired and prepared by 3cmX3cm 24h before each skin coating. And (3) sensitization induction, namely coating 0.5ml of test substance on filter paper, applying the filter paper to a hair removal area on the left side of an experimental group, covering gauze and oil paper, and sealing and fixing adhesive tapes for 6 hours. The same procedure is repeated for 7d and 14 d. Excitation treatment, namely removing hair and skin preparation 3cmX3cm on the right sides of the backs of the experimental group and the negative control group 14d after the last sensitization, taking 0.5ml of test object to apply to a skin preparation area by a sensitization contact method the next day, covering the test object with gauze and oilpaper, sealing and fixing an adhesive tape for 6 hours, and then washing off the applied test object. Skin reactions were observed after 24h and 48 h.

3. And (3) positive control, 2, 4-dinitrochlorobenzene is used as the positive control, the induction concentration and the excitation concentration are respectively 2.0% (w/v) and 1.0% (w/v), and the test steps are the same as those of the test group.

Thirdly, no skin reaction is seen by adopting a local closed skin coating method according to the test result, which is shown in the table.

Results of guinea pig skin allergy (sensitization) test

The skin erythema reaction intensity and the edema reaction intensity are animal ratio examples under the condition that the skin reaction integral is 0, 1, 2, 3 and 4

Fourth, conclusion

The skin sensitization rate (%) of the 5-time highest applied concentration solution to the test animal guinea pig is 0, and no skin allergy is found.

And (4) testing results:

1. acute oral toxicity test (mouse) the acute oral LDso values (95% confidence limit) of the samples for male and female mice are 5010 (3440-.

2. In a primary complete skin irritation test, the highest integral mean value of 5 times of the highest application concentration solution of the sample on primary complete skin irritation reaction of the rabbit is 0, and the irritation strength is nonirritant.

3. In the primary damaged skin irritation test, the highest integral mean value of the 5-time highest application concentration solution of the sample on the rabbit-secondary damaged skin irritation reaction is 0, and the irritation strength is nonirritant.

4. Multiple times of vagina mucosa irritation test, the average integral value of 5 times of the solution with the highest application concentration on the multiple times of vagina mucosa irritation test of rabbits is 7.77, and the test belongs to mild irritation

5. Acute eye irritation test, the sample solution with 5 times of the highest application concentration has no irritation to the acute eye irritation reaction of rabbits.

6. Skin allergy test, the skin sensitization rate (%) of the sample solution with the highest application concentration of 5 times to the guinea pig of the test animal is 0, and the skin allergy is not found.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.