阅读说明：本技术 一种复合底改菌的片剂及其制备方法 (Tablet of composite bottom modifying bacteria and preparation method thereof ) 是由 白琼 马家好 江文涛 马加军 于 2021-10-09 设计创作，主要内容包括：本发明属于微生物技术领域,具体涉及一种复合底改菌的片剂及其制备方法；该片剂由下述原辅料制备而成:枯草芽孢杆菌固体培养物4-5重量份,解淀粉芽孢杆菌固体培养物4-5重量份,元明粉40-60重量份,腐殖酸钠6-20重量份,白云石粉10-25重量份,沸石粉5-25重量份,麦芽糊精5-8重量份,硬脂酸镁0.5-1.0重量份。该片剂能快速提高池底氧化还原电位和溶解氧,分解池底淤泥,降解池底残饵、粪便、动植物尸体和有机碎屑,可有效吸附池底氨氮、亚硝酸盐、硫化氢、藻毒素等有害物质,降低水体酸度、稳定水体pH值,补充微量元素,提高水体透明度,达到改良底质、净化水质目的的片剂型复合底改菌及其制备方法。(The invention belongs to the technical field of microorganisms, and particularly relates to a compound bottom modifying bacterium tablet and a preparation method thereof; the tablet is prepared from the following raw and auxiliary materials, by weight, 4-5 parts of a bacillus subtilis solid culture, 4-5 parts of a bacillus amyloliquefaciens solid culture, 40-60 parts of anhydrous sodium sulphate, 6-20 parts of sodium humate, 10-25 parts of dolomite powder, 5-25 parts of zeolite powder, 5-8 parts of maltodextrin and 0.5-1.0 part of magnesium stearate. The tablet can rapidly improve the oxidation-reduction potential and dissolved oxygen of the bottom of the pool, decompose sludge at the bottom of the pool, degrade residual baits, excrement, animal and plant corpses and organic debris at the bottom of the pool, effectively adsorb harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide, algal toxin and the like at the bottom of the pool, reduce the acidity of a water body, stabilize the pH value of the water body, supplement trace elements, improve the transparency of the water body and achieve the purposes of improving the bottom and purifying the water quality.)

1. The tablet is characterized by being prepared from the following raw and auxiliary materials:

4-5 parts of bacillus subtilis solid culture, 4-5 parts of bacillus amyloliquefaciens solid culture, 40-60 parts of anhydrous sodium sulphate, 6-20 parts of sodium humate, 10-25 parts of dolomite powder, 5-25 parts of zeolite powder, 5-8 parts of maltodextrin and 0.5-1.0 part of magnesium stearate.

2. The compound amebocyte lysate tablet as claimed in claim 1, is prepared from the following raw and auxiliary materials: 4 parts of bacillus subtilis solid culture, 4 parts of bacillus amyloliquefaciens solid culture, 50 parts of anhydrous sodium sulphate, 13 parts of sodium humate, 15 parts of dolomite powder, 5 parts of zeolite powder, 8 parts of maltodextrin and 1.0 part of magnesium stearate.

3. The tablet of the composite bottom-modified bacteria of claim 1 or 2, wherein the viable count of the bacillus subtilis solid culture is 150 cfu/g.

4. The tablet of the composite bottom-modified bacteria of claim 1 or 2, wherein the viable count of the solid culture of the bacillus amyloliquefaciens is 150 cfu/g.

5. The tablet of the compound bottom modifying bacteria as claimed in any one of claims 1 to 4, wherein the preparation method of the tablet comprises the following steps:

(1) taking a bacillus subtilis solid culture, a bacillus amyloliquefaciens solid culture, anhydrous sodium sulphate, sodium humate, dolomite powder, zeolite powder and maltodextrin, and putting into a mixer to mix uniformly;

(2) and (3) adding magnesium stearate into the mixture obtained in the step (1), uniformly mixing, and tabletting to obtain tablets.

Technical Field

The invention relates to the technical field of microorganisms, in particular to a compound bottom modifying bacterium tablet and a preparation method thereof.

Background

The mud at the bottom of the pond is aged and the water quality is blackened and smelly due to the fact that the mud is not cleaned for many years, so that the aquatic animals such as fishes and shrimps cultured in the pond die. Eutrophication of the culture water body generally occurs: common dominant species such as increased concentration of dissolved or non-dissolved organic substances in pond water, increased content of nitrogen and phosphorus, pH value and biochemical oxygen consumption exceeding normal range, decreased transparency, green water color, diatom, etc. are replaced by dinoflagellate, etc. In severe cases, the eutrophication has spread to the water areas outside the ponds, and the ecological balance is seriously threatened. The blackening degree of the pond bottom is intensified, and almost more than half of the pond bottom is in a serious reduction state for a long time in the culture period, and the pond bottom is blackened and smelled abnormally and rapidly. Some occur locally, and some occur over a large area. The composition of the organisms at the bottom of the pool is poor, the diversity index is obviously reduced, and the benthos which can be used as the bait is almost extinct. This phenomenon is one of the causes of the aging of the pond bottom and is very unfavorable for the cultivation production. The bait utilization efficiency is reduced: during the cultivation process, on one hand, the quantity of residual baits is continuously increased, and on the other hand, the stomach emptying rate of the cultivated organisms is continuously increased. The vitality of the pond-cultured organisms is weakened, and the feed coefficient is improved year by year.

Based on the reasons, the method has important significance in the field of cultivation or the field of microorganisms for the research and development of the pool bottom modification technology of the cultivation pool.

Disclosure of Invention

The invention aims to solve the problems and provides a novel tablet of composite bottom modifying bacteria, which can rapidly improve the oxidation-reduction potential and dissolved oxygen of the bottom of a pool, decompose sludge at the bottom of the pool, degrade residual baits, feces, animal and plant corpses and organic debris at the bottom of the pool, effectively adsorb harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide, algal toxin and the like at the bottom of the pool, reduce the acidity of a water body, stabilize the pH value of the water body, supplement trace elements, improve the transparency of the water body and achieve the purposes of improving the bottom and purifying the water quality, and a preparation method thereof.

The invention is realized by the following technical scheme:

a tablet of compound bottom modifying bacteria is prepared from the following raw and auxiliary materials:

4-5 parts of bacillus subtilis solid culture, 4-5 parts of bacillus amyloliquefaciens solid culture, 40-60 parts of anhydrous sodium sulphate, 6-20 parts of sodium humate, 10-25 parts of dolomite powder, 5-25 parts of zeolite powder, 5-8 parts of maltodextrin and 0.5-1.0 part of magnesium stearate.

The preferable compound bottom modifying bacterium tablet is prepared from the following raw and auxiliary materials: 4 parts of bacillus subtilis solid culture, 4 parts of bacillus amyloliquefaciens solid culture, 50 parts of anhydrous sodium sulphate, 13 parts of sodium humate, 15 parts of dolomite powder, 5 parts of zeolite powder, 8 parts of maltodextrin and 1.0 part of magnesium stearate.

The viable count of the bacillus subtilis solid culture is 150 cfu/g.

The viable count of the bacillus amyloliquefaciens solid culture is 150 cfu/g.

The preparation method of the tablet comprises the following steps:

(1) taking a bacillus subtilis solid culture, a bacillus amyloliquefaciens solid culture, anhydrous sodium sulphate, sodium humate, dolomite powder, zeolite powder and maltodextrin, and putting into a mixer to mix uniformly;

(2) and (3) adding magnesium stearate into the mixture obtained in the step (1), uniformly mixing, and tabletting to obtain tablets.

The prepared tablet type composite bottom-modified bacteria is an off-white tablet, and the content of active ingredients is that the viable count is 10 cfu/g. The method is suitable for all breeding ponds and culture ponds of marine and freshwater aquatic animals in south and north, and is particularly suitable for microbial improvement of the pond bottom of a multi-sludge pond.

The application method of the tablet type composite bottom modifying bacteria comprises the step of directly and uniformly sprinkling the bottom modifying bacteria in the whole pool by a dry method.

The dosage of the tablet type composite bottom modifying bacterium is as follows:

1. the fertilizer is used after pond cleaning and before stocking, namely water is added for 10-20 cm after 5 days of pond cleaning and disinfection, and 200g of fertilizer is used per mu according to the aging condition of pond bottom, and the fertilizer is directly and uniformly sprayed on the pond bottom.

2. The use amount of the feed is 80-200 g per mu according to the aging condition of pond bottom.

The invention has the beneficial effects that:

the tablet type composite bottom modifying bacterium is a novel granular type composite bottom modifying bacterium which is formed by screening high-efficiency water purification viable bacteria, compounding ore powder with extremely strong adsorption capacity and indirect oxygenation and substrate modification substances. The product is in the form of tablet, has simple preparation process, high survival rate of viable bacteria, direct sprinkling, rapid bottom sinking, obvious effect and convenient use. The oxidation-reduction potential and dissolved oxygen at the bottom of the pool can be rapidly improved, sludge at the bottom of the pool can be decomposed, residual bait, excrement, animal and plant corpses and organic debris at the bottom of the pool can be degraded, ammonia nitrogen, nitrite, hydrogen sulfide, algal toxins and other harmful substances at the bottom of the pool can be effectively adsorbed, the acidity of the water body can be reduced, the pH value of the water body can be stabilized, trace elements can be supplemented, the transparency of the water body can be improved, and the purposes of improving the bottom and purifying the water quality can be achieved.

The tablet type composite bottom modifying bacterium has the following effects:

1. the substrate is improved, namely residual bait, excrement, animal and plant corpses and organic debris at the bottom of the pool are degraded;

2. increasing dissolved oxygen, namely increasing the oxidation-reduction potential and the dissolved oxygen at the bottom of the pool, promoting the mass propagation of aerobic probiotics and inhibiting the growth of anaerobic harmful bacteria;

3. purifying water quality, namely effectively adsorbing harmful substances such as ammonia nitrogen, nitrite, hydrogen sulfide, algal toxin and the like at the bottom of the tank;

4, improving the transparency of the water body and ensuring the normal operation of the photosynthesis of plankton in the water;

5. stabilizing the pH value of the water body, supplementing trace elements, keeping the proportion balance of probiotics and plankton in the water, and inhibiting the propagation and growth of harmful algae.

The tablet type composite bottom modifying bacterium has the following characteristics:

1. the product is a mechanically pressed granule preparation, has high survival rate of viable bacteria, can be directly sprayed, and is convenient for use.

2. The product has wide temperature range, and the living bacteria can grow and propagate at water temperature of 10-40 deg.C.

3. The product is suitable for water body bH6.0-10, and is suitable for various aquaculture water bodies.

4. The product has strong adaptability and strong tolerance to chemical disinfection drugs, insecticidal drugs, antibiotics and the like.

Detailed Description

The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.

Example 1

A tablet-type composite bottom-modifying bacterium has the following formula (namely, 1000 kg of raw materials required for producing the composite bottom-modifying bacterium) that 40kg of bacillus subtilis solid culture, 40kg of bacillus amyloliquefaciens solid culture, 500kg of anhydrous sodium sulphate, 130kg of sodium humate, 150kg of dolomite powder, 80kg of zeolite powder and 50kg of maltodextrin, and 10kg of magnesium stearate.

The preparation method of the tablet type composite bottom modifying bacterium comprises the following steps:

first, 40kg of bacillus subtilis solid culture, 40kg of bacillus amyloliquefaciens solid culture, 500kg of anhydrous sodium sulphate, 130kg of sodium humate, 150kg of dolomite powder and 80kg of zeolite powder 50kg of maltodextrin are mixed uniformly.

And (II) 10kg of magnesium stearate.

And (III) mixing and stacking the mixture obtained in the first step and the article obtained in the second step again, then putting the final mixture into a stirrer, uniformly mixing to meet the tabletting requirement, and finally putting into a tabletting machine to prepare the tablets.

The use effect test of the tablet type composite bottom modifying bacteria comprises the following steps:

the water body to be treated is 10 mu of fresh water shrimp pond on the water surface, the ammonia nitrogen is 1.0mg/L, the nitrite nitrogen is 0.5mg/mL, and the hydrogen sulfide is 0.2 mg/mL.

The treatment period is 6 months, the product is used for 1 time every 10 days in the first 3 months, 100g of the product is used for each mu of water surface (water depth is 1 meter), and the product is used for every 7 days in the last 3 months, and 200g of the product is used for each mu of water surface (water depth is 1 meter).

And after the treatment is finished, determining the water sample that the ammonia nitrogen is less than 0.05mg/L, the nitrite nitrogen is 0.05mg/mL, and the hydrogen sulfide is 0.05 mg/mL.

Example 2

A tablet-type composite bottom-modifying bacterium is prepared from solid culture of Bacillus subtilis (50 kg), solid culture of Bacillus amyloliquefaciens (40 kg), anhydrous sodium sulfate (400 kg), sodium humate (200 kg), dolomite powder (190 kg), zeolite powder (50 kg) and maltodextrin (60 kg) and magnesium stearate (10 kg).

The preparation method of the tablet type composite bottom modifying bacterium comprises the following steps:

50kg of bacillus subtilis solid culture, 40kg of bacillus amyloliquefaciens solid culture, 400kg of anhydrous sodium sulphate, 200kg of sodium humate, 190kg of dolomite powder and 60kg of zeolite powder, and uniformly mixing.

((II) magnesium stearate 10 kg.

And (III) mixing and stacking the mixture obtained in the first step and the product obtained in the second step again, then putting the final mixture into a stirrer, uniformly mixing to meet the tabletting requirement, and finally putting into a tabletting machine to prepare tablets.

The use effect test of the tablet type composite bottom modifying bacteria comprises the following steps:

the water body to be treated is fresh water fishpond with 25 mu of water surface, ammonia nitrogen is 1.8mg/L, nitrite nitrogen is 0.4mg/mL, and hydrogen sulfide is 0.2 mg/mL.

The treatment period is 6 months, the product is used for 1 time per 10 times in the first 3 months, 250g of the product is used for each mu of water surface (water depth is 1 m), and the product is used for every 7 days in the last 3 months, and 200g of the product is used for each mu of water surface (water depth is 1 m).

And after the treatment is finished, determining the water sample that the ammonia nitrogen is less than 0.05mg/L, the nitrite nitrogen is 0.01mg/mL, and the hydrogen sulfide is 0.05 mg/mL.

Example 3

A tablet-type composite bottom-modifying bacterium is prepared from solid culture of Bacillus subtilis (50 kg), solid culture of Bacillus amyloliquefaciens (50 kg), anhydrous sodium sulfate (600 kg), sodium humate (100 kg), dolomite powder (100 kg), zeolite powder (20 kg), maltodextrin (70 kg) and magnesium stearate (10 kg).

The preparation method of the tablet type composite bottom modifying bacterium comprises the following steps:

50kg of bacillus subtilis solid culture, 50kg of bacillus amyloliquefaciens solid culture, 600kg of anhydrous sodium sulphate, 100kg of sodium humate, 100kg of dolomite powder and 70kg of zeolite powder 20kg of maltodextrin, and uniformly mixing.

And (II) taking 10kg of magnesium stearate.

And (III) mixing and stacking the mixture obtained in the first step and the article obtained in the second step again, then putting the final mixture into a stirrer, uniformly mixing to meet the tabletting requirement, and finally putting into a tabletting machine to prepare the tablets.

The use effect test of the tablet type composite bottom modifying bacteria comprises the following steps:

the water body to be treated is a seawater shrimp pond with 80 mu of water surface, the ammonia nitrogen is 1.4mg/L, the nitrite nitrogen is 0.5mg/mL, and the hydrogen sulfide is 0.25 mg/mL.

The treatment period is 6 months, the product is used 1 time every 10 days in the first 3 months, and 100g of the product is used on each mu of water surface (the water depth is 1 meter); the product is applied once every 7 days in the last 3 months, and 200g of the product is applied to each mu of water surface (the water depth is 1 meter).

And after the treatment is finished, determining the water sample that the ammonia nitrogen is less than 0.1mg/L, the nitrite nitrogen is 0.01mg/mL, and the hydrogen sulfide is 0.02 mg/mL.

The tablet form composite bottom modifying bacteria have obvious improvement effect on aquaculture water environment, and the tablet form composite bottom modifying bacteria are tablets, have strong slow release effect and can treat different harmful substances in different water depths, so that the high-efficiency effect is achieved, and the treatment effect is good.

Preparation examples include, but are not limited to, those described above.