Electrical stimulation cell culture device

文档序号:1932503 发布日期:2021-12-07 浏览:16次 中文

阅读说明:本技术 一种电刺激细胞培养装置 (Electrical stimulation cell culture device ) 是由 卢博涛 姬帅 王江 叶宇星 马壮 焦鑫 朱雷 贾帅军 郝定均 于 2021-09-01 设计创作,主要内容包括:本发明公开了一种电刺激细胞培养装置:包括培养皿以及设于培养皿外部底端的电刺激板,所述电刺激板下端设有电刺激仪,所述电刺激板通过导电组件与电刺激仪连接,所述电刺激仪为沙发椅状,所述电刺激仪的靠背段内侧与培养皿外壁接触连接,所述电刺激仪上端设有电刺激监测屏,所述电刺激仪一侧设有开关按钮。所述培养皿与电刺激板通过透明胶水粘合。所述电刺激板包括正、负两个电极,所述电刺激板为氧化铟锡(ITO)镀层导电玻璃材料。所述电刺激仪另一侧设有电源接口,所述电刺激仪内部设有控制模块。当需要使用时,电刺激仪对电刺激板进行刺激,通过电刺激对接种在培养皿的细胞进行培养养,能有效加快细胞生长和增殖。(The invention discloses an electrical stimulation cell culture device, which comprises: including the culture dish and locate the electro photoluminescence board of the outside bottom of culture dish, the electro photoluminescence board lower extreme is equipped with the electro photoluminescence appearance, the electro photoluminescence board passes through conductive component and is connected with the electro photoluminescence appearance, the electro photoluminescence appearance is the sofa chair form, the inboard and culture dish outer wall contact connection of back section of electro photoluminescence appearance, the electro photoluminescence appearance upper end is equipped with the electro photoluminescence monitoring screen, electro photoluminescence appearance one side is equipped with shift knob. The culture dish and the electric stimulation plate are bonded through transparent glue. The electric stimulation plate comprises a positive electrode and a negative electrode, and is made of an Indium Tin Oxide (ITO) coating conductive glass material. The other side of the electrical stimulation instrument is provided with a power interface, and a control module is arranged in the electrical stimulation instrument. When the electric stimulation plate needs to be used, the electric stimulation instrument stimulates the electric stimulation plate, cells inoculated in the culture dish are cultured through electric stimulation, and the growth and proliferation of the cells can be effectively accelerated.)

1. An electrical stimulation cell culture device, characterized in that: including culture dish (1) and locate electro photoluminescence board (2) of culture dish (1) outside bottom, electro photoluminescence board (2) lower extreme is equipped with electro photoluminescence appearance (3), electro photoluminescence board (2) are connected with electro photoluminescence appearance (3) through conductive component, electro photoluminescence appearance (3) are the sofa form, the back section inboard and culture dish (1) outer wall contact of electro photoluminescence appearance (3) are connected, electro photoluminescence appearance (3) upper end is equipped with electro photoluminescence and detects screen (4), electro photoluminescence detects screen (4) rear end and is equipped with three operation key (10), electro photoluminescence appearance (3) one side is equipped with shift knob (5).

2. An electrically stimulated cell culture device according to claim 1, wherein: the culture dish (1) is bonded with the electric stimulation plate (2) through transparent glue.

3. An electrically stimulated cell culture device according to claim 1, wherein: the electric stimulation plate (2) comprises a positive electrode and a negative electrode, and the electric stimulation plate (2) is made of an Indium Tin Oxide (ITO) coating conductive glass material.

4. An electrically stimulated cell culture device according to claim 1, wherein: and a power interface (6) is arranged on the other side of the electrical stimulator (3).

5. An electrically stimulated cell culture device according to claim 1, wherein: the electric stimulation instrument (3) comprises an electromagnetic wave generation module and a clock module.

6. An electrically stimulated cell culture device according to claim 1, wherein: the culture dish (1) comprises a culture dish cover (7).

7. An electrically stimulated cell culture device according to claim 1, wherein: the electric stimulation instrument (3) is provided with a handle (8).

8. An electrically stimulated cell culture device according to claim 1, wherein: and a sounding hole (9) is formed in one side, close to the power interface (6), of the electrical stimulator (3).

Technical Field

The invention relates to the technical field of culture devices, in particular to an electrical stimulation cell culture device.

Background

Cell culture (cellcell) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to make it survive, grow, reproduce and maintain the main structure and function.

In medical treatment, treatment and rehabilitation after spinal cord injury and peripheral nerve injury are difficult problems which plague clinicians, so that the search for a method for accelerating nerve regeneration is particularly important, and previous researches show that electrical stimulation can effectively promote the growth of neuron axons and the proliferation of a plurality of glial cells in the processes of spinal cord injury repair and peripheral nerve repair so as to accelerate the injury repair, so that the design of a proper cell electrical stimulation device has great significance for researching mechanisms and principles of spinal cord injury treatment and peripheral nerve regeneration.

Disclosure of Invention

The technical problem to be solved by the invention is to overcome the defects of the technology and provide an electrical stimulation cell culture device.

In order to solve the technical problems, the technical scheme provided by the invention is that the electrical stimulation cell culture device comprises: including the culture dish and locate the electro photoluminescence board of the outside bottom of culture dish, electro photoluminescence board lower extreme is equipped with the electro photoluminescence appearance, the electro photoluminescence board passes through conductive component and is connected with the electro photoluminescence appearance, the electro photoluminescence appearance is the sofa chair form, the inboard and culture dish outer wall contact connection of back section of electro photoluminescence appearance, the electro photoluminescence appearance upper end is equipped with the electro photoluminescence monitoring screen, the electro photoluminescence is surveyed the screen rear end and is equipped with three operation key, electro photoluminescence appearance one side is equipped with shift knob.

As a modification, the culture dish and the electric stimulation plate are bonded through transparent glue.

As an improvement, the electric stimulation plate comprises a positive electrode and a negative electrode, and the electric stimulation plate is made of an Indium Tin Oxide (ITO) coating conductive glass material.

As an improvement, the other side of the electrical stimulation instrument is provided with a power interface.

As an improvement, the electrical stimulation instrument comprises an electromagnetic wave generation module and a clock module.

As a refinement, the culture dish comprises a culture dish cover.

As an improvement, the electric stimulation instrument is provided with a handle.

As an improvement, one side of the electrical stimulation instrument, which is close to the power interface, is provided with a sound emitting hole.

Compared with the prior art, the invention has the advantages that: when the electric stimulation instrument is used, the electric stimulation instrument stimulates the electric stimulation plate, cells inoculated in the culture dish are cultured and raised through electric stimulation, the growth and proliferation of the cells can be effectively accelerated, a research means is provided for further researching the influence and action mechanism of the electric stimulation on various cells, the electric stimulation instrument can provide waveforms including square waves, sine waves, exponential waves and triangular waves, the current magnitude (0-10mA), the frequency (0-1000HZ), the operation key can be set, and the timing function is achieved. The electrical stimulation cell culture device is efficient and safe, and has high practical value and popularization value.

Drawings

FIG. 1 is a schematic structural diagram of an electrical stimulation cell culture device according to the present invention.

FIG. 2 is a second schematic structural view of an electro-stimulation cell culture device according to the present invention.

As shown in the figure: 1. culture dish, 2, electro photoluminescence board, 3, electro photoluminescence appearance, 4, electro photoluminescence monitoring screen, 5, shift knob, 6, power source, 7, culture dish lid, 8, handle, 9, pronunciation hole, 10, operation key.

Detailed Description

An electrical stimulation cell culture apparatus according to the present invention will be described in further detail with reference to the accompanying drawings.

With reference to the attached drawings 1-2, the electrical stimulation cell culture device comprises a culture dish 1 and an electrical stimulation plate 2 arranged at the bottom end outside the culture dish 1, wherein an electrical stimulation instrument 3 is arranged at the lower end of the electrical stimulation plate 2, the electrical stimulation plate 2 is connected with the electrical stimulation instrument 3 through a conductive component, the electrical stimulation instrument 3 is in a sofa shape, the inner side of a backrest section of the electrical stimulation instrument 3 is in contact connection with the outer wall of the culture dish 1, an electrical stimulation monitoring screen 4 is arranged at the upper end of the electrical stimulation instrument 3, three operation keys 10 are arranged at the rear end of the electrical stimulation monitoring screen 4, and a switch button 5 is arranged on one side of the electrical stimulation instrument 3.

The culture dish 1 is bonded with the electric stimulation plate 2 through transparent glue.

The electric stimulation plate 2 comprises a positive electrode and a negative electrode, and the electric stimulation plate 2 is made of an Indium Tin Oxide (ITO) coating conductive glass material.

And a power interface 6 is arranged on the other side of the electrical stimulator 3.

The electrical stimulator 3 includes an electromagnetic wave generating module and a clock module.

The culture dish 1 comprises a culture dish lid 7.

The electrostimulator 3 is provided with a handle 8.

And one side of the electrical stimulation instrument 3 close to the power interface 6 is provided with a sound emitting hole 9.

When the electric stimulation device is used in concrete implementation, the electric stimulation instrument 3 stimulates the electric stimulation plate 2, cells inoculated in a culture dish are cultured and raised through electric stimulation, the growth and proliferation of the cells can be effectively accelerated, a research means is provided for further researching the influence and action mechanism of the electric stimulation on various cells, the electric stimulation instrument 3 can provide waveforms including square waves, sine waves, exponential waves, triangular waves and the like, the current magnitude (0-10mA), the frequency (0-1000HZ), the operation key 10 can be set, and the timing function is also provided.

The electrical stimulation cell culture device is efficient and safe, and has high practical value and popularization value.

The present invention and its embodiments have been described above, and the description is not intended to be limiting, and the drawings are only one embodiment of the present invention, and the actual structure is not limited thereto. In summary, those skilled in the art should appreciate that they can readily use the disclosed conception and specific embodiments as a basis for designing or modifying other structures for carrying out the same purposes of the present invention without departing from the spirit and scope of the invention as defined by the appended claims.

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