阅读说明：本技术 一种细胞培养皿 (Cell culture dish ) 是由 赵宝玉 杨庆 于 2021-09-23 设计创作，主要内容包括：本发明涉及培养皿技术领域,具体为一种细胞培养皿,包括皿身与上盖,上盖扣装在皿身的上表面,且皿身与上盖的上表面左侧均开设有进液槽,且开设在皿身上表面的进液槽贯穿皿身,本发明在使用时,由于设置有进液槽、环形斜槽以及竖槽,当需要向培养皿中补充培养液时,仅需要向进液槽中灌入培养液,培养液沿着进液槽进入到环形斜槽内,然后沿着竖槽流入皿身内部,从而能够对培养液进行补充,并且在培养液补充的过程中,无需打开上盖,从而避免了空气中的杂质落入培养液中,造成培养液的污染,另外,培养液经过进液槽、环形斜槽以及竖槽进入到培养皿中,相对于直接将培养液倒入培养皿中,能够减小培养液对于细胞的冲击,从而避免细胞被冲散甚至损坏。(The invention relates to the technical field of culture dishes, in particular to a cell culture dish, which comprises a dish body and an upper cover, wherein the upper cover is buckled on the upper surface of the dish body, the left sides of the upper surfaces of the dish body and the upper cover are both provided with a liquid inlet groove, and the liquid inlet groove arranged on the upper surface of the dish body penetrates through the dish body. Can reduce the impact of the culture solution on the cells, thereby avoiding the cells from being scattered and even damaged.)

1. A cell culture dish, includes dish body (1) and upper cover (2), its characterized in that: the upper cover (2) is buckled on the upper surface of the dish body (1), a liquid inlet groove (3) is formed in the left side of the side wall of the dish body (1) and the upper surface of the upper cover (2), the liquid inlet groove (3) formed in the upper surface of the dish body (1) penetrates through the dish body (1), an annular chute (4) is formed in the inner surface of the dish body (1), the bottom of the liquid inlet groove (3) formed in the side wall of the dish body (1) and the upper surface of the upper cover (2) is communicated with the annular chute (4), an aligning mechanism (8) is arranged at the joint of the dish body (1) and the left side of the outer surface of the upper cover (2), vertical grooves (5) which are uniformly distributed in an annular array are formed in the inner surface of the dish body (1), the vertical grooves (5) are communicated with the annular chute (4), a liquid outlet (6) is formed in the bottom of the right side of the outer surface of the dish body (1), and a rubber plug (7) is movably connected to the opening of the liquid outlet (6), and a sealing mechanism (9) is arranged on the upper surface of the upper cover (2).

2. A cell culture dish according to claim 1, wherein: the aligning mechanism (8) comprises a first fixing block (81), a guide pillar (82) and a second fixing block (83), the first fixing block (81) is fixedly installed at the position, close to the joint of the dish body (1) and the upper cover (2), of the left side of the outer surface of the dish body (1), the second fixing block (83) is fixedly installed at the position, close to the joint of the dish body (1) and the upper cover (2), of the left side of the outer surface of the upper cover (2), the middle position of the lower surface of the second fixing block (83) is fixedly connected with the guide pillar (82), a cylindrical groove matched with the guide pillar (82) is formed in the middle position of the upper surface of the first fixing block (81), and the guide pillar (82) is embedded inside the cylindrical groove.

3. The cell culture dish of claim 1, wherein the sealing mechanism (9) comprises a circular plate (91), a rotating shaft (92), a circular hole (93), a fixing column (94) and a limiting groove (95), the rotating shaft (92) is rotatably connected to the middle position of the upper surface of the upper cover (2), the circular plate (91) is fixedly connected to the upper surface of the rotating shaft (92), the circular hole (93) with the same diameter as the liquid inlet groove (3) is formed in the left side of the upper surface of the circular plate (91), the limiting groove (95) is formed in the position, far away from the circular hole (93), of the left side of the upper surface of the circular plate (91), the fixing column (94) is slidably mounted inside the limiting groove (95), and the lower surface of the fixing column (94) is fixedly connected with the upper cover (2).

4. A cell culture dish according to claim 1, wherein: the middle position of the inner surface of the upper cover (2) is fixedly connected with an inclined table (10), the outer surface of the inclined table (10) is provided with guide grooves (11) which are uniformly distributed, the middle position of the lower surface of the inclined table (10) is fixedly connected with a second connecting rod (17), the lower end of the outer surface of the second connecting rod (17) is a threaded part, a threaded sleeve (18) is sleeved on the lower end thread of the second connecting rod (17), the middle position of the lower surface of the threaded sleeve (18) is fixedly connected with a first connecting rod (16), the lower surface of the first connecting rod (16) is fixedly connected with a collecting cylinder (15), the right side of the outer surface of the collecting cylinder (15) is fixedly connected with a connecting hose (12) in a through manner, the right side of the outer surface of the upper cover (2) is provided with a connecting groove (13), and one end, far away from the collecting cylinder (15), of the connecting hose (12) is slidably mounted inside the connecting groove (13), and a switch mechanism (14) is arranged inside the connecting groove (13).

5. A cell culture dish according to claim 4, wherein: switch mechanism (14) are including weight (141), logical groove (142), baffle (143) and through-hole (144), logical groove (142) of running through connecting groove (13) is seted up on upper cover (2) lower surface right side, logical groove (142) inside slidable mounting has baffle (143), through-hole (144) the same with connecting groove (13) shape are seted up to baffle (143) surface intermediate position, the last fixed surface of baffle (143) is connected with weight (141), just the top and the weight (141) of logical groove (142) cooperate, the bottom and the baffle (143) of logical groove (142) cooperate.

6. A cell culture dish according to claim 4, wherein: spout (21) have been seted up to the surface bilateral symmetry of second connecting rod (17), spout (21) inside slidable mounting has slider (20), the surface intermediate position fixedly connected with U type pole (19) of slider (20), U type pole (19) run through second connecting rod (17) and sliding fit with it, just the other end of U type pole (19) supports threaded sleeve (18).

Technical Field

The invention relates to the technical field of culture dishes, in particular to a cell culture dish.

Background

A petri dish is a laboratory vessel for the cultivation of microorganisms or cells, consisting of a flat disc-shaped bottom and a lid, generally made of glass or plastic. The culture dish is basically made of two types, mainly plastic and glass, and the glass can be used for plant materials, microorganism culture and adherent culture of animal cells. The plastic may be of polyethylene material, disposable and reusable, suitable for laboratory inoculation, streaking, bacterial isolation and may be used for the cultivation of plant material.

Among the current cell culture process, because the culture solution in the culture dish constantly consumes, need artifical supplementary culture solution to the culture dish in, with the required nutrition of assurance cell growth in-process, however, during the artifical culture solution that supplements, need open the upper cover of culture dish, pour into the culture solution in to the culture dish again, because the culture dish keeps opening, impurity in the air falls into the culture dish easily, lead to the culture solution to receive the pollution, influence the production metabolism of cell, in addition, when pouring into the culture solution in the culture dish, the culture solution of pouring into causes the impact to the cell in the culture dish, can break away the cell that has already adhered to the wall, influence the normal propagation of cell, can cause the death of cell seriously.

For this purpose, a cell culture dish is proposed.

Disclosure of Invention

The present invention is directed to a cell culture dish to solve the above problems of the background art.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a cell culture dish, includes ware body and upper cover, the upper cover lock dress is at the upper surface of ware body, just the feed liquor groove has all been seted up on the lateral wall of ware body and the upper surface left side of upper cover, and has seted up the feed liquor groove of ware body upper surface runs through the ware body, annular chute has been seted up to the internal surface of ware body, and has seted up the feed liquor groove and the annular chute of ware body lateral wall and upper cover upper surface are linked together, ware body and upper cover surface left side junction are provided with alignment mechanism, the interior surface of ware body has been seted up and has been annular array evenly distributed's perpendicular groove, just perpendicular groove all communicates with each other with annular chute, the liquid outlet has been seted up to the surface right side bottom of ware body, just liquid outlet opening part swing joint has the plug, the upper surface of upper cover is provided with closing mechanism.

In the working process, in the existing cell culture process, because the culture solution in the culture dish is continuously consumed, the culture solution needs to be supplemented into the culture dish manually to ensure the nutrition required in the cell growth process, however, when the culture solution is supplemented manually, the upper cover of the culture dish needs to be opened, the culture solution is poured into the culture dish, and because the culture dish is kept open, impurities in the air easily fall into the culture dish to pollute the culture solution and influence the production and metabolism of cells, in addition, when the culture solution is poured into the culture dish, the poured culture solution impacts the cells in the culture dish, and the cells are scattered and even damaged. The culture solution enters into annular chute along the feed liquor groove in, then flow into inside the ware body along perpendicular groove, thereby can supply the culture solution, and at the in-process that the culture solution was supplied, need not to open the upper cover, thereby impurity in having avoided the air falls into the culture solution, cause the pollution of culture solution, in addition, the culture solution passes through the feed liquor groove, annular chute and perpendicular groove enter into the culture dish, pour the culture solution into the culture dish for directly, can reduce the impact of culture solution to the cell, thereby avoid scattering the cell of adherence, influence the normal reproduction of cell, cause the death of cell.

Preferably, the aligning mechanism comprises a first fixing block, a guide pillar and a second fixing block, the first fixing block is fixedly mounted at a position close to the joint of the dish body and the upper cover on the left side of the outer surface of the dish body, the second fixing block is fixedly mounted at a position close to the joint of the dish body and the upper cover on the left side of the outer surface of the upper cover, the guide pillar is fixedly connected to the middle position of the lower surface of the second fixing block, a cylindrical groove matched with the guide pillar is formed in the middle position of the upper surface of the first fixing block, and the guide pillar is embedded in the cylindrical groove.

The during operation, when closing upper cover and ware body, feed liquor groove on the upper cover and the ware body feed liquor groove on one's body may stagger, lead to unable carry the culture solution to the culture dish in, through setting up alignment mechanism, when closing upper cover and ware body, only need with the guide post plug-in mounting on the second fixed block in the cylindrical groove on the first fixed block, just can guarantee that the feed liquor groove on the upper cover aligns with the feed liquor groove on one's body, thereby the culture solution is carried to the culture dish through the feed liquor groove.

Preferably, the closing mechanism includes plectane, pivot, round hole, fixed column and spacing groove, the upper surface intermediate position of upper cover rotates and is connected with the pivot, the last fixed surface of pivot is connected with the plectane, just the round hole the same with feed liquor groove diameter is seted up in the upper surface left side of plectane, the position department of keeping away from the round hole in the upper surface left side of plectane has seted up the spacing groove, the inside slidable mounting of spacing groove has the fixed column, the lower surface and the upper cover fixed connection of fixed column.

When the culture dish is operated, after the culture fluid is supplemented into the culture dish through the fluid inlet groove, impurities in the air can be attached to the inner wall of the fluid inlet groove because the fluid inlet groove is communicated with the outside, when the fluid supplement is carried out again, the impurities enter the interior of the culture dish along with the culture fluid to cause pollution of the culture dish, and the growth and development of cells are influenced, through the arrangement of the sealing mechanism, after the culture fluid is supplemented into the culture dish, because the circular plate is rotatably connected with the upper cover through the rotating shaft, through the rotation of the circular plate, the circular hole on the circular plate is staggered with the fluid inlet groove, the fluid inlet groove is blocked by the circular plate, the fluid inlet groove is separated from the outside, the impurities in the air are prevented from being attached to the inner wall of the fluid inlet groove, the culture fluid is prevented from being polluted, in addition, through the arrangement of the limiting groove and the fixing column, the rotation angle of the circular plate can be controlled, when the fixing column rotates to one side of the limiting groove, the circular hole on the circular plate is aligned with the fluid inlet groove, when the fixed column rotates to the other side of the limiting groove, the round hole on the circular plate is staggered with the liquid inlet groove.

Preferably, the upper cover internal surface intermediate position fixedly connected with sloping platform, evenly distributed's guiding gutter has been seted up to the sloping platform surface, the lower surface intermediate position fixedly connected with second connecting rod of sloping platform, the surface lower extreme of second connecting rod is screw thread portion, just the lower extreme screw thread cover of second connecting rod is equipped with threaded sleeve, threaded sleeve's lower surface intermediate position fixedly connected with head rod, the lower fixed surface of head rod is connected with the surge drum, the fixed through connection in surface right side of surge drum has coupling hose, the spread groove has been seted up on the surface right side of upper cover, coupling hose keeps away from the one end slidable mounting of surge drum inside the spread groove, just inside being provided with operating mechanism of spread groove.

In the working process, in the process of culturing cells, cell metabolism can generate water vapor, the water vapor is attached to the inner surface of the upper cover to form water drops, the water drops can disperse the cells and even damage the cells, the water vapor generated by the cell metabolism is attached to the surface of the sloping platform and flows into the collecting cylinder along with the flow guide groove to prevent the water drops from dropping and dispersing the cells, and the cells are damaged The water vapor generated by cell metabolism is collected by the diversion trench and the collecting cylinder, so that the water drops are prevented from falling to scatter the cells and damaging the cells.

Preferably, the switch mechanism includes the weight, leads to groove, baffle and through-hole, the logical groove that runs through the spread groove is seted up on upper cover lower surface right side, it has the baffle to lead to the inside slidable mounting of groove, the through-hole the same with the spread groove shape is seted up to baffle surface intermediate position, the last fixed surface of baffle is connected with the weight, just the top that leads to the groove cooperatees with the weight, the bottom and the baffle that lead to the groove cooperate.

During operation, before upper cover and ware body are closed, because the spread groove communicates with each other with the external world, outside air accessible spread groove enters into in the connecting hose, cause the coupling hose to pollute, through setting up on-off mechanism, baffle and weight receive the effect of self gravity, make baffle and weight move down, through-hole and spread groove on the baffle stagger, separate coupling hose and external world, prevent that the coupling hose from receiving the pollution, after upper cover and ware body are closed, baffle and weight receive the extrusion rebound of ware body, make through-hole and spread groove on the baffle communicate with each other, can discharge the water in the surge drum through the spread groove.

Preferably, the spout has been seted up to the surface bilateral symmetry of second connecting rod, the inside slidable mounting of spout has the slider, the surface intermediate position fixedly connected with U type pole of slider, U type pole runs through the second connecting rod and with it sliding fit, just the other end of U type pole supports the threaded sleeve.

When the water collecting device works, when the water in the collecting cylinder is gradually increased, the gravity of the water in the collecting cylinder and the collecting cylinder is increased, the bearing of the joint of the threaded sleeve and the second connecting rod is increased, the connection between the threaded sleeve and the second connecting rod is unstable, the U-shaped rod fixedly connected with the sliding block is moved by sliding the sliding block to one side of the sliding groove before the threaded sleeve is connected with the second connecting rod, so that the U-shaped rod does not obstruct the connection between the threaded sleeve and the second connecting rod, after the threaded sleeve is connected with the second connecting rod, the sliding block is slid to the other side of the sliding groove, the U-shaped rod fixedly connected with the sliding block is moved, the bottom of the U-shaped rod supports the threaded sleeve, when the gravity of the water in the collecting cylinder and the collecting cylinder is increased, the U-shaped rod can share a part of bearing, so that the bearing of the joint of the threaded sleeve and the second connecting rod is reduced, avoid threaded sleeve and second connecting rod to be connected unstablely.

Compared with the prior art, the invention has the beneficial effects that:

1. when the culture dish is used, due to the arrangement of the liquid inlet groove, the annular chute and the vertical groove, when culture liquid needs to be supplemented into the culture dish, the culture liquid only needs to be poured into the liquid inlet groove, enters the annular chute along the liquid inlet groove and then flows into the dish body along the vertical groove, so that the culture liquid can be supplemented, and in the culture liquid supplementing process, an upper cover does not need to be opened, so that the culture liquid is prevented from being polluted due to the fact that impurities in the air fall into the culture liquid.

2. In addition, the culture solution enters into the culture dish through feed liquor groove, annular chute and perpendicular groove, for directly pouring the culture solution into the culture dish in, can reduce the impact of culture solution to the cell to avoid the cell to be dispersed even damage.

Drawings

FIG. 1 is a schematic structural view of the present invention as a whole;

FIG. 2 is a cross-sectional view of the present invention;

FIG. 3 is a structural view of the alignment mechanism of the present invention;

FIG. 4 is a structural view of the closure mechanism of the present invention;

FIG. 5 is an enlarged view taken at A of FIG. 2 in accordance with the present invention;

FIG. 6 is a cross-sectional view of a second connecting rod of the present invention;

fig. 7 is a structural view of the switch mechanism of the present invention.

In the figure: 1. a dish body; 2. an upper cover; 3. a liquid inlet tank; 4. an annular chute; 5. a vertical slot; 6. a liquid outlet; 7. a rubber plug; 8. an alignment mechanism; 81. a first fixed block; 82. a guide post; 83. a second fixed block; 9. a sealing mechanism; 91. a circular plate; 92. a rotating shaft; 93. a circular hole; 94. fixing a column; 95. a limiting groove; 10. a sloping table; 11. a diversion trench; 12. a connecting hose; 13. connecting grooves; 14. a switch mechanism; 141. a weighting block; 142. a through groove; 143. a baffle plate; 144. a through hole; 15. a collection canister; 16. a first connecting rod; 17. a second connecting rod; 18. a threaded sleeve; 19. a U-shaped rod; 20. a slider; 21. a chute.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", may explicitly or implicitly include one or more of the described features. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise. Furthermore, the terms "mounted," "connected," and "connected" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1 to 7, the present invention provides a technical solution:

a cell culture dish is shown in figures 1-2, and comprises a dish body 1 and an upper cover 2, wherein the upper cover 2 is buckled on the upper surface of the dish body 1, and the side wall of the dish body 1 and the left side of the upper surface of the upper cover 2 are both provided with a liquid inlet groove 3, the liquid inlet groove 3 arranged on the upper surface of the dish body 1 penetrates through the dish body 1, the inner surface of the dish body 1 is provided with an annular chute 4, and a liquid inlet groove 3 arranged on the side wall of the dish body 1 and the upper surface of the upper cover 2 is communicated with an annular chute 4, an alignment mechanism 8 is arranged at the left connecting part of the outer surfaces of the dish body 1 and the upper cover 2, vertical grooves 5 which are uniformly distributed in an annular array are arranged on the inner surface of the dish body 1, the vertical grooves 5 are communicated with the annular chute 4, a liquid outlet 6 is arranged at the bottom of the right side of the outer surface of the dish body 1, and the opening of the liquid outlet 6 is movably connected with a rubber plug 7, and the upper surface of the upper cover 2 is provided with a sealing mechanism 9.

In the working process, in the existing cell culture process, because the culture solution in the culture dish is continuously consumed, the culture solution needs to be supplemented into the culture dish manually to ensure the nutrition required in the cell growth process, however, when the culture solution is supplemented manually, the upper cover 2 of the culture dish needs to be opened, the culture solution is poured into the culture dish, because the culture dish is kept open, impurities in the air easily fall into the culture dish, the culture solution is polluted, the production and metabolism of cells are influenced, in addition, when the culture solution is poured into the culture dish, the poured culture solution impacts the cells in the culture dish, the cells are scattered or even damaged, when the invention is used, because the liquid inlet groove 3, the annular chute 4 and the vertical groove 5 are arranged, when the culture solution needs to be supplemented into the culture dish, the rubber plug 7 is firstly taken down, and the waste liquid in the culture dish is discharged from the liquid outlet 6, irritate the culture solution again to feed liquor groove 3, the culture solution enters into annular chute 4 along feed liquor groove 3 in, then flow into 1 inside of ware body along perpendicular 5 grooves, thereby can supply the culture solution, and at the in-process that the culture solution was supplied, need not to open upper cover 2, thereby impurity in having avoided the air falls into the culture solution, cause the pollution of culture solution, in addition, the culture solution passes through feed liquor groove 3, annular chute 4 and perpendicular 5 grooves enter into the culture dish, for directly pouring the culture solution into the culture dish in, can reduce the impact of culture solution to the cell, thereby avoid dashing and scattering the cell that has adherence, influence the normal reproduction of cell, cause the death of cell.

As an embodiment of the present invention, as shown in fig. 3, the alignment mechanism 8 includes a first fixing block 81, a guide pillar 82, and a second fixing block 83, the first fixing block 81 is fixedly installed at a position on the left side of the outer surface of the dish body 1 near the connection between the dish body 1 and the upper cover 2, the second fixing block 83 is fixedly installed at a position on the left side of the outer surface of the upper cover 2 near the connection between the dish body 1 and the upper cover 2, the guide pillar 82 is fixedly connected to the middle position of the lower surface of the second fixing block 83, a cylindrical groove adapted to the guide pillar 82 is formed in the middle position of the upper surface of the first fixing block 81, and the guide pillar 82 is embedded in the cylindrical groove.

During operation, when closing upper cover 2 and ware body 1, feed liquor groove 3 on upper cover 2 and the feed liquor groove 3 on the ware body 1 may stagger, lead to unable in carrying the culture solution to the culture dish, through setting up alignment mechanism 8, when closing upper cover 2 and ware body 1, only need advance the column-shaped inslot on the first fixed block 81 with guide pillar 82 cartridge on the second fixed block 83, just can guarantee that feed liquor groove 3 on the upper cover 2 aligns with feed liquor groove 3 on the ware body 1, thereby the culture solution is carried to the culture dish through feed liquor groove 3.

As an embodiment of the present invention, as shown in fig. 4, the sealing mechanism 9 includes a circular plate 91, a rotating shaft 92, a circular hole 93, a fixing column 94 and a limiting groove 95, the rotating shaft 92 is rotatably connected to the middle position of the upper surface of the upper cover 2, the circular plate 91 is fixedly connected to the upper surface of the rotating shaft 92, the circular hole 93 having the same diameter as the liquid inlet groove 3 is formed in the left side of the upper surface of the circular plate 91, the limiting groove 95 is formed in the position far from the circular hole 93 in the left side of the upper surface of the circular plate 91, the fixing column 94 is slidably installed in the limiting groove 95, and the lower surface of the fixing column 94 is fixedly connected to the upper cover 2.

In operation, after the culture solution is supplemented into the culture dish through the liquid inlet groove 3, impurities in the air may be attached to the inner wall of the liquid inlet groove 3 because the liquid inlet groove 3 is communicated with the outside, when the liquid supplement is performed again, the impurities enter the culture dish along with the culture solution to cause pollution of the culture dish, and the growth and development of cells are influenced, through the arrangement of the sealing mechanism 9, after the culture solution is supplemented into the culture dish, because the circular plate 91 is rotatably connected with the upper cover 2 through the rotating shaft 92, the circular hole 93 on the circular plate 91 is staggered with the liquid inlet groove 3 through the rotation of the circular plate 91, the liquid inlet groove 3 is blocked by the circular plate 91, the liquid inlet groove 3 is separated from the outside, the impurities in the air are prevented from being attached to the inner wall of the liquid inlet groove 3, the culture solution is prevented from being polluted, in addition, through the arrangement of the limiting groove 95 and the fixing column 94, the rotation angle of the circular plate 91 can be controlled, when the fixing column 94 rotates to one side of the limiting groove 95, the round hole 93 on the circular plate 91 is aligned with the liquid inlet groove 3, and when the fixing column 94 rotates to the other side of the limiting groove 95, the round hole 93 on the circular plate 91 is staggered with the liquid inlet groove 3.

As an embodiment of the present invention, as shown in fig. 2, a ramp 10 is fixedly connected to a middle position of the inner surface of the upper cover 2, the outer surface of the sloping platform 10 is provided with guide grooves 11 which are uniformly distributed, the middle position of the lower surface of the sloping platform 10 is fixedly connected with a second connecting rod 17, the lower end of the outer surface of the second connecting rod 17 is a threaded part, a threaded sleeve 18 is sleeved on the lower end of the second connecting rod 17 through threads, a first connecting rod 16 is fixedly connected to the middle position of the lower surface of the threaded sleeve 18, the lower surface of the first connecting rod 16 is fixedly connected with a collecting cylinder 15, the right side of the outer surface of the collecting cylinder 15 is fixedly connected with a connecting hose 12 in a penetrating way, connecting groove 13 has been seted up on the surface right side of upper cover 2, the one end slidable mounting that collecting vessel 15 was kept away from to coupling hose 12 is inside connecting groove 13, just inside being provided with operating device 14 of connecting groove 13.

In the process of culturing cells, water vapor is generated by cell metabolism and is attached to the inner surface of the upper cover 2 to form water drops, the water drops fall to disperse the cells and even damage the cells, the water vapor generated by the cell metabolism is attached to the surface of the inclined table 10 and flows into the collecting cylinder 15 along with the flow guide groove 11 to prevent the water drops formed by the water vapor from falling to disperse the cells and damage the cells, in addition, the connecting hose 12 is arranged to discharge the water of the collecting cylinder 15 from the connecting groove 13 through the connecting hose 12, after the cells are cultured, the equipment needs to be cleaned, when the collecting cylinder 15 needs to be cleaned, the connecting hose 12 only needs to be pulled out from the connecting groove 13, the collecting cylinder 15 is rotated to separate the threaded sleeve 18 from the second connecting rod 17, namely, the collecting cylinder 15 can be detached, thereby make things convenient for the cleanness of a collection section of thick bamboo 15, through setting up sloping platform 10, guiding gutter 11 and a collection section of thick bamboo 15, collect the steam that the cell metabolism produced, prevent that the water droplet from falling and rushing away the cell, cause the damage to the cell.

As an embodiment of the present invention, as shown in fig. 7, the opening and closing mechanism 14 includes a weight 141, a through groove 142, a blocking plate 143, and a through hole 144, the right side of the lower surface of the upper cover 2 is provided with the through groove 142 penetrating through the connecting groove 13, the blocking plate 143 is slidably installed inside the through groove 142, the through hole 144 having the same shape as the connecting groove 13 is formed in the middle of the outer surface of the blocking plate 143, the weight 141 is fixedly connected to the upper surface of the blocking plate 143, the top of the through groove 142 is engaged with the weight 141, and the bottom of the through groove 142 is engaged with the blocking plate 143.

In operation, before upper cover 2 and dish body 1 are closed, because connecting groove 13 communicates with each other with the external world, outside air accessible connecting groove 13 enters into coupling hose 12, cause coupling hose 12 to pollute, through setting up switching mechanism 14, baffle 143 and weight 141 receive the effect of self gravity, make baffle 143 and weight 141 move down, through-hole 144 on baffle 143 staggers with connecting groove 13, separate coupling hose 12 with the external world, prevent coupling hose 12 from polluting, after upper cover 2 and dish body 1 are closed, baffle 143 and weight 141 receive the extrusion of dish body 1 and move upwards, make through-hole 144 on baffle 143 communicate with each other with connecting groove 13, can discharge the water in the collection section of thick bamboo 15 through connecting groove 13.

As an embodiment of the present invention, as shown in fig. 6, sliding grooves 21 are symmetrically formed on both sides of an outer surface of the second connecting rod 17, a sliding block 20 is slidably mounted inside the sliding grooves 21, a U-shaped rod 19 is fixedly connected to a middle position of the outer surface of the sliding block 20, the U-shaped rod 19 penetrates through the second connecting rod 17 and is slidably engaged with the second connecting rod, and the other end of the U-shaped rod 19 abuts against the threaded sleeve 18.

In operation, when the water in the collecting cylinder 15 increases gradually, the gravity of the collecting cylinder 15 and the water in the collecting cylinder 15 increases, the load of the joint of the threaded sleeve 18 and the second connecting rod 17 is increased, so that the connection between the threaded sleeve 18 and the second connecting rod 17 is unstable, by providing the U-shaped rod 19, before the threaded sleeve 18 is connected with the second connecting rod 17, the sliding block 20 is slid to one side of the sliding slot 21, the U-shaped rod 19 fixedly connected with the sliding block 20 is moved, so that the U-shaped rod 19 does not interfere with the connection between the threaded sleeve 18 and the second connecting rod 17, after the threaded sleeve 18 is connected with the second connecting rod 17, by sliding the sliding block 20 to the other side of the sliding slot 21, the U-shaped rod 19 fixedly connected with the sliding block 20 is moved, so that the threaded sleeve 18 is supported by the bottom of the U-shaped rod 19, and when the gravity of the water in the collecting cylinder 15 and the collecting cylinder 15 increases, the U-shaped rod 19 can share a part of the load, therefore, the load at the joint of the threaded sleeve 18 and the second connecting rod 17 is reduced, and the unstable connection between the threaded sleeve 18 and the second connecting rod 17 is avoided.

The using method comprises the following steps: in the existing cell culture process, because the culture solution in the culture dish is continuously consumed, the culture solution needs to be supplemented into the culture dish manually to ensure the nutrition required in the cell growth process, however, when the culture solution is supplemented manually, the upper cover 2 of the culture dish needs to be opened, the culture solution is poured into the culture dish, because the culture dish is kept open, impurities in the air easily fall into the culture dish, the culture solution is polluted, the production and metabolism of cells are influenced, in addition, when the culture solution is poured into the culture dish, the poured culture solution impacts the cells in the culture dish, the cells are scattered and even damaged, when the invention is used, because the liquid inlet groove 3, the annular chute 4 and the vertical groove 5 are arranged, when the culture solution needs to be supplemented into the culture dish, the culture solution only needs to be poured into the liquid inlet groove 3, and enters the annular chute 4 along the liquid inlet groove 3, then flow into 1 insidely of ware body along vertical groove 5, thereby can supply the culture solution, and at the in-process that the culture solution supplyed, need not to open upper cover 2, thereby impurity in having avoided the air falls into the culture solution, cause the pollution of culture solution, in addition, the culture solution passes through feed liquor groove 3, annular chute 4 and vertical groove 5 and enters into the culture dish, for directly pouring the culture solution into the culture dish in, can reduce the impact of culture solution to the cell, thereby avoid the cell to be broken up or even damage.

The electric elements in the document are electrically connected with an external main controller and 220V mains supply through a transformer, the main controller can be a conventional known device controlled by a computer and the like, the product model provided by the invention is only used according to the structural characteristics of the product, the product can be adjusted and modified after being purchased, so that the product is more matched with and accords with the technical scheme of the invention, the product model is a technical scheme of the optimal application of the technical scheme, the product model can be replaced and modified according to the required technical parameters, and the product model is familiar to the technical personnel in the field, so that the technical scheme provided by the invention can clearly obtain the corresponding use effect.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.