阅读说明：本技术 存储富血小板血浆的设备 (Device for storing platelet-rich plasma ) 是由 米特拉·纳贾菲 于 2020-03-06 设计创作，主要内容包括：本公开提供一种存储富血小板血浆(PRP)的设备。该设备被构造为可逆地容纳富血小板血浆(PRP)容器。该设备包括：平台,限定至少一个凹槽,该凹槽被构造为在其中容纳PRP容器；以及搅拌器,被构造为移动该平台,从而搅拌PRP容器中存储的PRP。该搅拌器被构造为以10至10000转每分钟(RPM)的频率以圆周运动来移动平台。(The present disclosure provides an apparatus for storing Platelet Rich Plasma (PRP). The device is configured to reversibly hold a Platelet Rich Plasma (PRP) container. The apparatus comprises: a platform defining at least one recess configured to receive a PRP container therein; and an agitator configured to move the platform, thereby agitating the PRP stored in the PRP container. The agitator is configured to move the platform in a circular motion at a frequency of 10 to 10000 Revolutions Per Minute (RPM).)

1. An apparatus for storing Platelet Rich Plasma (PRP), the apparatus configured to reversibly hold a Platelet Rich Plasma (PRP) container, and comprising:

a platform defining at least one recess configured to receive the PRP container therein; and

an agitator configured to move the platform to agitate the PRP stored in the PRP container, wherein the agitator is configured to move the platform in a circular motion at a frequency of 10 to 10000 Revolutions Per Minute (RPM).

2. The apparatus of claim 1, wherein the platform defines a plurality of recesses, wherein each recess is configured to receive the PRP container therein.

3. The apparatus of claim 1 or 2, wherein the apparatus comprises a holder configured to hold the PRP container in the groove.

4. The apparatus of claim 3, wherein the retainer comprises a biasing device configured to bias the PRP container against a first side of the groove.

5. The apparatus of claim 4, wherein the first side of the groove defines an angle between 10 ° and 35 °.

6. The apparatus of claim 3 or 4, wherein the biasing device comprises a spring, wherein a proximal end of the spring is fixedly attached to a second side of the groove and the spring is configured to bias the PRP container against a first side of the groove, wherein the second side is opposite the first side.

7. The apparatus of claim 6, wherein the spring defines an angle of 0 ° to 20 ° from horizontal.

8. The apparatus of any preceding claim, wherein the agitator is configured to move the platform in a circular motion at a frequency between 50 and 1000RPM, between 100 and 750RPM, between 150 and 500RPM, between 200 and 400RPM or between 250 and 450 RPM.

9. The apparatus of any preceding claim, wherein the agitator is further configured to agitate the PRP stored in the PRP container by being configured to move the PRP container in a linear motion.

10. The apparatus according to any preceding claim, wherein the agitator is configured to continuously agitate the PRP stored in the PRP container.

11. The apparatus of any one of claims 1 to 9, wherein the agitator is configured to intermittently agitate the PRP stored in the PRP container.

12. The apparatus of any preceding claim, wherein the apparatus comprises a lid configured to fit over the platform and any PRP container disposed thereon.

13. The apparatus according to any preceding claim, wherein the apparatus comprises a temperature controller configured to maintain the temperature of the PRP stored in the PRP container between 15 ℃ and 30 ℃, between 16 ℃ and 28 ℃, between 17 ℃ and 27 ℃, between 18 ℃ and 26 ℃, between 19 ℃ and 25 ℃, or between 20 ℃ and 24 ℃.

14. The device of any preceding claim, wherein the device is configured to be connected to a mains power supply.

15. The apparatus of any preceding claim, wherein the apparatus comprises a battery configured to drive the agitator.

16. A kit for storing PRP, the kit comprising a device as defined in any preceding claim and a PRP container.

17. A method of storing PRPs, the method comprising:

placing the PRP in a PRP container; and is

Agitating the PRP in a circular motion at a frequency of 10 to 10000 Revolutions Per Minute (RPM).

Technical Field

The present invention relates to an apparatus configured as a container for storing Platelet Rich Plasma (PRP). The invention extends to kits comprising the apparatus and the container, as well as to the container itself. The invention also relates to a method of storing PRP.

Background

Platelets are circulating megakaryocytes whose mother cells are anucleated cytoplasmic debris that are confined to the hematopoietic compartment of bone. 2000-3000 platelets were produced per megakaryocyte. While platelets are often thought to play a major role in hemostasis, they are intricately involved in the development of wound healing. Platelets have a complex, specific structure and physiology, although only cellular debris.

As a concentrated source of platelets, plasma contains several cytokines that stimulate tissue regeneration and have anti-aging activity. Plasma can be obtained by centrifuging blood until the main mixture of blood (red blood cells, white blood cells and plasma) separates. The platelet-rich portion of plasma is referred to as platelet-rich plasma (PRP) or fraction 2(F2), while the platelet-poor portion of plasma is referred to as platelet-poor plasma (PPP) or fraction 1 (F1).

The prominent role platelets play in wound healing and the relative ease with which PRP is produced and used in clinical or surgical settings has led to an increasing use of PRP to promote regeneration of soft and hard tissues. In particular, autologous PRP has been used to treat chronic skin and soft tissue ulceration, maxillofacial surgery, orthopedic and traumatic surgery, orthopedic surgery, spinal surgery, cardiac bypass surgery, and burns. It is also used in dentistry for various purposes such as oral mucosa healing, root canal regeneration and scaffolds for pulp regeneration.

Local delivery of a single growth factor (delivery), usually recombinant platelet-derived growth factor BB (PDGF-BB), has also been employed. However, the use of PRP represents a greater similarity to the natural healing process and allows the application of multiple wound healing promoters in biologically determined proportions. In addition, the short shelf life of recombinant PDGF-BB, its cost and concern about side effects make PRP an attractive and cost-effective proposition.

However, once obtained, PRP has a relatively short shelf life. This is due in part to the fact that platelets are anucleate, and therefore even in vivo, they have a lifetime of only 8-10 days. However, in vitro samples tend to have shorter shelf lives due to platelet aggregation. Accordingly, there is a need for improved apparatus and methods for storing PRP.

Disclosure of Invention

The present invention results from work by the inventors seeking to overcome the problems associated with the prior art.

According to a first aspect of the present invention, there is provided an apparatus for storing Platelet Rich Plasma (PRP), the apparatus being configured to reversibly receive a Platelet Rich Plasma (PRP) container and comprising:

a platform defining at least one recess configured to receive a PRP container therein; and

an agitator configured to move the platform to agitate the PRP stored in the PRP container, wherein the agitator is configured to move the platform in a circular motion at a frequency of 10 to 10,000 Revolutions Per Minute (RPM).

Advantageously, the inventors have found that the agitator reduces activation and debris formation within the PRP.

Preferably, the platform defines a plurality of recesses, wherein each recess is configured to receive a PRP container therein. Thus, the apparatus may be configured to reversibly accommodate a plurality of PRP containers.

The platform can define at least 2, at least 4, at least 6, at least 8, at least 10, at least 12, or at least 8 recesses, wherein each recess is configured to receive a PRP container therein. The platform may define 1 to 100 recesses, 2 to 50 recesses, 3 to 25 recesses, or 5 to 15 recesses, wherein each recess is configured to receive a PRP container therein. The inventors have found that PRP can be stored for up to eight days using the apparatus of the first aspect. Thus, in one embodiment, the equipment platform defines 8 recesses, wherein each recess is configured to receive a PRP container therein. Accordingly, each container may contain a PRP that one person will use on a single day. Optionally, the apparatus may include 14 recesses, wherein each recess is configured to receive a PRP container therein. Accordingly, this would provide a weekly PRP supply for users who are used twice a day in batches

The apparatus may include a retainer configured to retain the PRP container in the groove. Advantageously, the retainer prevents displacement of the PRP container, thereby preventing spillage or leakage. The retainer may include a biasing device configured to bias the PRP container against the first side of the groove. Advantageously, the biasing means retains the container in the recess. The first side of the groove may define an angle between 0 ° and 90 ° to the vertical, more preferably an angle between 5 ° and 45 ° to the vertical, an angle between 10 ° and 35 ° to the vertical, or an angle between 15 ° and 30 ° to the vertical, and most preferably an angle between 18 ° and 25 ° to the vertical, an angle between 19 ° and 22.5 ° to the vertical, or an angle between 20 ° and 21 ° to the vertical.

The biasing means may comprise a spring, wherein a proximal end of the spring is fixedly attached to a second side of the groove and the spring is configured to bias the PRP container against a first side of the groove, wherein the second side is opposite the first side. Preferably, the spring defines an angle between 0 ° and 20 ° to the horizontal, more preferably between 0 ° and 10 ° to the horizontal, and most preferably between 0 ° and 5 ° to the horizontal or between 0 ° and 2.5 ° to the horizontal. Advantageously, when a user inserts the container into the recess at an angle defined by the second side of the recess, the base of the container will urge the spring towards the second side of the recess to allow the base to pass the spring.

The biasing device may further comprise a contact member disposed on a distal end of the spring. The contact member may define a surface that matches the profile of the PRP container. Thus, the contact member may define a flat or concave surface. Advantageously, the contact member increases the contact area with the PRP container. The surface may define an angle between 0 ° and 90 ° to the vertical, more preferably an angle between 5 ° and 45 ° to the vertical, an angle between 10 ° and 35 ° to the vertical or an angle between 15 ° and 30 ° to the vertical, and most preferably an angle between 18 ° and 25 ° to the vertical, an angle between 19 ° and 22.5 ° to the vertical or an angle between 20 ° and 21 ° to the vertical.

It will be appreciated that the above-described angle may be defined when the device is disposed on a flat horizontal surface.

In embodiments where the platform comprises a plurality of grooves, the apparatus may comprise a retainer for each groove. Each holder may be defined as above.

The agitator may include a speed controller. When the agitator is activated, the speed controller may be configured to gradually increase the agitation frequency until the target frequency is reached. The speed controller may be configured to increase the stirring frequency by 0.001 to 100 seconds, 0.01 to 10 seconds, or 0.1 to 1 second. Advantageously, this may reduce stress within the device.

The agitator may be configured to move the platform in a circular motion at a frequency of 50 to 1000RPM, more preferably 100 to 750RPM or 150 to 500RPM, and most preferably 200 to 400RPM or 250 to 450 RPM. The inventors have found that a stirring speed of about 300RPM is particularly advantageous for storing PRP.

The agitator may include a motor including a rotor and a stator. The agitator may include a cam provided on the rotor. The platform may be coupled to the cam. Thus, the motor will cause the platform to move in a circle. The apparatus may be configured to prevent rotation of the platform. The apparatus may include a bearing disposed between the cam and the platform. Advantageously, the bearing reduces friction between the cam and the platform.

The agitator may also be configured to agitate the PRP stored in the PRP container by being configured to move the PRP container in a linear motion.

The apparatus may be configured to continuously agitate the PRP stored in the PRP container. Alternatively, the apparatus may be configured to intermittently agitate the PRP stored in the PRP container. Thus, the apparatus may be configured to switch between a first configuration in which the agitator is activated and a second configuration in which the agitator is not activated. The apparatus may be configured to blend PRP stored in the PRP container for at least 10% of the storage time, more preferably for at least 20% of the storage time, for at least 30% of the storage time or for at least 40% of the storage time, and most preferably for at least 50% of the storage time. The apparatus may be configured to blend PRP stored in the PRP container for up to less than 90% of the storage time, more preferably for up to less than 80% or for up to less than 70% of the storage time, and most preferably for up to less than 60% of the storage time. The apparatus may be configured to blend PRP stored in the PRP container for up to 10% to 90% of the storage time, more preferably up to 20% to 80% or up to 30 to 70% of the storage time, and most preferably up to 40% to 60% of the storage time. The storage time may be defined as the time at which the PRP is stored in a PRP container and the container is set on the platform. Advantageously, the inventors have found that intermittent stirring can reduce activation and debris formation within the PRP, while reducing power consumption.

Preferably, the time period between stirring is less than 24 hours, more preferably less than 20 hours, less than 15 hours or less than 10 hours, and most preferably less than 5 hours, less than 2 hours, less than 1.5 hours, less than 1.25 hours or less than 1 hour. Preferably, the time period between stirring is at least 5 minutes, more preferably at least 10 minutes, at least 15 minutes or at least 20 minutes, and most preferably at least 30 minutes, at least 40 minutes, at least 50 minutes or at least 60 minutes. Preferably, the time period between stirring is between 5 minutes and 24 hours, more preferably between 10 minutes and 20 hours, between 15 minutes and 15 hours or between 20 minutes and 10 hours, and most preferably between 30 minutes and 5 hours, between 40 minutes and 120 minutes, between 50 minutes and 90 minutes or between 60 minutes and 75 minutes. The time period between agitation may be considered the length of time that the apparatus is continuously in the second configuration.

Thus, the apparatus may be configured to perform a predetermined stirring pattern. The predetermined stirring pattern may be understood as being defined by a length of time the stirrer is configured to be in the first configuration and a length of time the stirrer is configured to be in the second configuration. The agitator is preferably configured to repeat a predetermined agitation pattern.

It will be appreciated that in embodiments where the apparatus is configured to stir the PRP stored in the PRP container for up to 40% to 60% of the storage time and the time period between stirring is less than 4 hours, the device may be configured to switch between the first configuration and the second configuration every two hours, i.e. it may be configured to stir the PRP container for two hours and then not stir the PRP container for two hours.

The device may include a housing. The agitator may be disposed inside the housing. The platform may be disposed substantially adjacent to the housing. The housing may include an upper edge. Preferably, the upper edge defines a projection extending substantially around its circumference. Preferably, the platform is disposed substantially adjacent the upper edge. The platform may include a flange extending substantially around an outer circumference of the platform. Preferably, the flange is disposed substantially adjacent the projection. Preferably, the flange is disposed substantially around the projection. Advantageously, the protrusion and the flange reduce dust from entering the housing.

The apparatus may include a damping means disposed within the housing. The damping means may be configured to absorb vibrations, preferably noise vibrations. The damping means may comprise a natural or synthetic rubber or a cushioning material. The synthetic rubber may comprise neoprene. The pad may comprise cotton wool. The damping means may be disposed between the agitator and the platform. Advantageously, the damping means is configured to reduce noise generated by the apparatus.

The apparatus may include a lid configured to fit over the platform and any PRP container disposed thereon. The housing may be shaped to reversibly receive a portion of the lid. Preferably, the lid is airtight. Advantageously, the cover prevents dust and other environmental contaminants that may be present in the air from contacting the PRP container. The cover may include an integrated handle. Advantageously, the user can pick up the stirring device by means of the handle and transport the stirring device.

The apparatus may be configured to prevent inadvertent removal of the lid from above the platform. The cap may include threads. The platform or housing may include corresponding threads. Advantageously, the cap can be screwed into place, preventing inadvertent removal. Alternatively or additionally, the apparatus may include a child resistant lock configured to prevent removal of the lid from the platform and/or housing. The lid may include a tab (tab) such that the lid is removed when twisted relative to the platform and/or housing and a force is applied to the tab.

Alternatively, the apparatus may include a stirring portion including a stirrer, and a containing portion configured to reversibly contain the stirring portion. In embodiments where an agitation portion is present, the agitation portion may include a housing. The receptacle may include a base configured to reversibly receive the stirring portion and a cover configured to reversibly mount over the base when the stirring portion is disposed therein. Advantageously, the containment portion prevents dust and other environmental contaminants that may be present in the air from contacting the stirring portion and the PRP container. Preferably, the tank is airtight. The receiving portion may include a handle. Advantageously, the handle enables the user to more easily carry and transport the device.

The housing, platform, cover and/or receptacle may comprise plastic. The housing, platform, lid and/or receptacle may be injection molded. The plastic may comprise a thermoplastic polymer. The plastic may comprise Acrylonitrile Butadiene Styrene (ABS), polyethylene, polycarbonate, polyamide, polystyrene, or polypropylene. The polystyrene may be a high impact polystyrene.

The stirrer may comprise aluminium, brass and/or steel.

The apparatus may include a temperature controller configured to maintain the PRP stored in the PRP container at a temperature of at least 15 ℃, more preferably at least 16 ℃, at least 17 ℃ or at least 18 ℃, and most preferably at least 19 ℃ or at least 20 ℃. Preferably, the temperature controller is configured to maintain the PRP stored in the PRP container at a temperature below 30 ℃, more preferably below 29 ℃, below 28 ℃, below 27 ℃ or below 26 ℃, and most preferably below 25 ℃ or below 24 ℃. The temperature controller may be configured to maintain the PRP stored in the PRP container between 15 ℃ and 30 ℃, more preferably between 16 ℃ and 28 ℃, between 17 ℃ and 27 ℃, or between 18 ℃ and 26 ℃, and most preferably between 19 ℃ and 25 ℃ or between 20 ℃ and 24 ℃.

The temperature controller may be configured to control the temperature of the region between the platform and the lid, thereby maintaining the PRP stored in the PRP container at a desired temperature as defined herein. Alternatively or additionally, the temperature controller may be configured to control the temperature within the receptacle.

The temperature controller may include a temperature sensor configured to sense a temperature. The temperature sensor may be configured to sense a temperature within the recess. Therefore, the temperature sensor may be exposed in the groove. Alternatively or additionally, the temperature sensor may be configured to sense a temperature between the platform and the lid.

The temperature controller may comprise a proportional-integral-derivative (PID) controller.

The device may include a clock. The clock may include a microchip configured to hold time. The clock may be disposed in the housing.

In some embodiments, the temperature controller may include a heater. The heater may be configured to activate when the sensed temperature is below a predetermined minimum temperature. The heater may be configured to heat the recess and/or the area between the platform and the lid when activated. The predetermined minimum temperature may be a temperature of at least 15 ℃. More preferably, the predetermined minimum temperature is a temperature between 16 ℃ and 24 ℃, a temperature between 17 ℃ and 23 ℃ or a temperature between 18 ℃ and 22 ℃, and most preferably the predetermined minimum temperature is between 19 ℃ and 21 ℃. The heater may be configured to stop operating when a predetermined minimum temperature is reached.

However, the inventors have found that the stirrer generates heat. Thus, in some embodiments, a separate heater is not required. In embodiments where the apparatus is configured to intermittently agitate the PRP stored in the PRP container, the apparatus may be configured to switch the apparatus to the first configuration if the sensed temperature is below a predetermined minimum temperature. Thus, if the sensed temperature is below the predetermined minimum temperature, the agitator may be configured to override the predetermined agitation mode and activate the agitator. The predetermined minimum temperature may be defined as above. The apparatus may be configured to revert to a predetermined stirring mode when a predetermined minimum temperature is reached.

The temperature controller may comprise a cooler. The cooler may be configured to activate when the sensed temperature is above a predetermined maximum temperature. The cooler may comprise a fan. The fan may be disposed in the receptacle, and preferably in a base of the receptacle. Accordingly, the cooler may be configured to circulate air within the receiving portion. The cooler may be configured to cool the recess and/or the area between the platform and the lid when it is activated. The predetermined maximum temperature may be a temperature between 20 ℃ and 30 ℃, a temperature between 21 ℃ and 28 ℃ or a temperature between 22 ℃ and 26 ℃, and most preferably the predetermined minimum temperature is between 23 ℃ and 25 ℃. The cooler may be configured to stop operating when a predetermined maximum temperature is reached.

Thus, the apparatus may be configured to maintain the PRP stored in the PRP container at a temperature of between 15 ℃ and 29 ℃, more preferably at a temperature of between 16 ℃ and 28 ℃, 17 ℃ to 27 ℃, or between 18 ℃ and 26 ℃, and most preferably between 19 ℃ and 25 ℃ or between 20 ℃ and 24 ℃.

The device may be configured to be connected to a mains power supply. Advantageously, the mains power supply may drive the stirrer and/or the temperature controller. Preferably, the mains power supply may drive the stirrer and the temperature controller.

Alternatively or additionally, the apparatus comprises a battery configured to drive the agitator and/or the temperature controller. Preferably, the battery is configured to drive the agitator and the temperature controller.

In a preferred embodiment, the device is configured to be connected to a mains power supply and comprises a battery. Preferably, the battery is a rechargeable battery and the mains power supply can recharge the battery. Advantageously, the device can be driven for long periods of time from mains supplied power without the need to replace or recharge the batteries. However, batteries may be used if there is a power outage or if the device is remote from the mains power supply. This ensures that the stirrer and temperature controller can be operated continuously without interruption.

The device may include a display configured to convey information to a user. The display may be configured to inform the user whether the device (a) is using mains power; (b) using a battery to supply power; (c) stirring at a target frequency; and/or (d) maintaining the PRP at the target temperature. The display may include one or more LED lights.

The apparatus may include a transmission module. The transmission module may be configured to transmit the notification to the receiver. The transmission module may be a wireless transmission module and may be configured to wirelessly transmit the notification to the receiver. The transmission module may be configured to useThe notification may be transmitted via a technology, via a Radio Frequency (RF) signal, via a wireless internet network, and/or using Near Field Communication (NFC) technology. The receiver may be a mobile communication device.

The transmission module is configured to be interrupted during (a) the supply of power from the mains power supply; (b) the battery is below a predetermined charge; (c) the temperature sensor detects that the temperature is higher than a preset maximum temperature; and/or (d) transmitting a notification to the receiver when the temperature sensor detects a temperature below a predetermined minimum temperature. The predetermined maximum and minimum temperatures may be defined as above. The predetermined charge amount may be 50% or less of the full charge amount, 40% or less of the full charge amount, 30% or less of the full charge amount, 20% or less of the full charge amount, or 10% or less of the full charge amount. The receiver may be configured to display a message when it receives a notification from the transmission module. Advantageously, the user will be informed whether the device is at risk of losing power and/or whether it is too hot or too cold, and appropriate action can be taken.

Alternatively or additionally, the transmission module may be configured to continuously transmit the notification to the receiver while the device is supplying power. The receiver may be configured to display a message if the notification is interrupted. Advantageously, if the device is powered off, the user will be notified and appropriate action can be taken.

Alternatively or additionally, the apparatus may comprise an alarm configured to be activated when (a) the supply of electrical power from the mains power supply is interrupted; (b) the battery is below a predetermined charge; (c) the temperature sensor detects that the temperature is higher than a preset maximum temperature; and/or (d) the alarm sounds when the temperature sensor detects a temperature below a predetermined minimum temperature.

The apparatus may include a memory. The memory may include a memory card disposed inside the housing. Optionally, the device may include a connector configured to connect the device to an external memory. The connector may include a USB port. Alternatively, the connector may be a transmission module. The external memory may include a USB memory stick, a server, or a mobile communication device. The device may be configured to store data to the memory. The data may include a temperature sensed by a temperature sensor. The data may further include the time at which the temperature was sensed. The apparatus may be configured to store the temperature sensed by the temperature sensor at certain time intervals. The specific time interval may be less than 48 hours, more preferably less than 24 hours, less than 18 hours, or less than 12 hours, and most preferably less than 10 hours, less than 8 hours, or less than 6 hours. The specific time interval may be at least 5 minutes, more preferably at least 30 minutes, at least 1 hour, or at least 2 hours, and most preferably at least 3 hours, at least 4 hours, or at least 5 hours. The specific time interval may be between 5 minutes and 48 hours, more preferably between 30 minutes and 24 hours, between 1 hour and 18 hours or between 2 hours and 12 hours, and most preferably between 3 hours and 10 hours, between 4 hours and 8 hours or between 5 hours and 6 hours. Advantageously, the user can confirm that the temperature remains within the desired range.

The apparatus may include a base configured to rest on a flat surface. In a preferred embodiment, the base comprises a non-slip material. The non-slip material may be rubber. Advantageously, the rubber base prevents the device from slipping and reduces noise caused by the stirrer.

The apparatus may include a weight. The weight may be disposed toward or substantially adjacent the base of the housing. Advantageously, the weight will stabilize the device. How much the weight of the stirrer should be to ensure stability? "C (B)

According to a second aspect, there is provided a kit (kit) for storing PRP, the kit comprising the device of the first aspect and a PRP container.

The PRP container can include plastic. The plastic may be polypropylene.

The PRP container can be configured to allow oxygen permeation therein. Optionally, the container may be configured to prevent oxygen permeation. In a preferred embodiment, the PRP container is configured to allow oxygen permeation therein.

The PRP container can include a lid. The cover may be removable.

The kit may include a plurality of PRP containers. Preferably, the kit comprises at least one PRP container for each recess. Advantageously, each PRP container can be configured to include an appropriate amount of PRP for one treatment. The suitable amount of PRP may be between 0.01ml and 10ml, more preferably between 0.5ml and 5ml or between 0.1ml and 2.5ml, most preferably between 0.2ml and 1ml or between 0.4ml and 0.6 ml.

The kit may include a container including an activator configured to activate the PRP. The activator may comprise collagen.

The kit may include a plurality of activator containers comprising an activator. The kit may comprise the same number of PRP containers and activator containers. Each activator vessel may comprise an amount of activator suitable for one treatment. The appropriate amount of activator may be between 0.01ml and 25ml, more preferably between 0.5ml and 10ml or between 0.1ml and 5ml, and most preferably between 0.25ml and 3ml or between 0.5ml and 2 ml. Advantageously, a user may combine the contents of a PRP container and an activator container immediately prior to use without measuring a particular amount.

According to a third aspect, there is provided a method of storing PRP, the method comprising:

placing a PRP in a PRP container; and is

The PRP is agitated in a circular motion at a frequency of 10 to 10000 Revolutions Per Minute (RPM).

Agitating the PRP can include moving the PRP container in a circular motion. The PRP container can be moved in a circular motion at a frequency of 50 to 1000RPM, more preferably 100 to 750RPM or 150 to 500RPM, and most preferably between 200 to 400RPM or 250 to 450 RPM.

Alternatively or additionally, agitating the PRP can include moving the PRP container in a linear motion.

The method may comprise maintaining the PRP at a temperature of at least 15 ℃, at least 16 ℃, at least 17 ℃ or at least 18 ℃, and most preferably at a temperature of at least 19 ℃ or at least 20 ℃. The process may comprise maintaining the PRP at a temperature of less than 30 ℃, less than 29 ℃ or less than 28 ℃, and more preferably at a temperature of less than 27 ℃ or less than 26 ℃, and most preferably at a temperature of less than 25 ℃ or less than 24 ℃.

The method may comprise maintaining the PRP at a temperature of between 15 ℃ and 29 ℃, more preferably at a temperature of between 16 ℃ and 28 ℃, 17 ℃ to 27 ℃, or 18 ℃ to 26 ℃, and most preferably between 19 ℃ and 25 ℃ or between 20 ℃ and 24 ℃.

The PRP can be stirred and maintained at the desired temperature at the same time. The PRP can be stirred and maintained at the desired temperature for at least 12 hours, more preferably at least 1 day, at least 2 days, at least 3 days, or at least 4 days, and most preferably at least 5 days, at least 6 days, at least 7 days, or at least 8 days.

The PRP container may be as defined in relation to the second aspect.

All of the features disclosed herein (including any accompanying claims, abstract and drawings), and/or all of the steps of any method or process so disclosed, may be combined with any of the above aspects, in any combination, except combinations where at least some of such mutually exclusive features and/or steps are present.

Drawings

For a better understanding of the present invention, and to show how embodiments thereof may be carried into effect, reference will now be made, by way of example, to the accompanying drawings, in which:

fig. 1 is a perspective view of an apparatus configured as a container for storing Platelet Rich Plasma (PRP);

FIG. 2 is a cross-sectional view of the apparatus;

FIG. 3 is a perspective view of the components of the apparatus;

FIG. 4 is a side view of other components of the apparatus;

FIG. 5 is a further cross-sectional view of the apparatus;

FIG. 6 is an exploded view of the apparatus;

FIG. 7a is a perspective view of an apparatus configured to store four PRP containers; FIG. 7b is a perspective view of an apparatus configured to store eight PRP containers; FIG. 7c is a perspective view of an apparatus configured to store fifteen PRP containers; and

fig. 8 is a perspective view of an apparatus configured to store fourteen PRP containers.

Detailed Description

Example 1: storage device

An apparatus 2 configured to store a container 4 comprising a PRP is shown in fig. 1 to 6. As shown in fig. 1, the apparatus 2 includes a case 6, a top bracket 8, and a cover 10. The top bracket 8 is disposed above and adjacent the bin 6 and, as discussed in more detail below, defines a recess 12 sized to receive the container 4 therein. The lid 10 is configured to be reversibly mounted over the top bracket 8.

The case comprises two parts, an upper case member 14 and a lower case member 16, which are assembled together to define the case 6. The lower box 16 comprises a substantially flat base 18 to allow the apparatus 2 to rest on a flat surface. The base 18 includes a rubber pad 20 disposed on the outside of the base 18 to prevent the device from slipping.

As can be seen in fig. 2, the upper box member 14 includes an outer wall 22 and an inner wall 24. The lower housing member 16 and the outer and inner walls 22, 24 of the upper housing member 14 define a lower chamber 26. Similarly, top bracket 8 and inner wall 24 of upper box member 14 define an upper chamber 28. Lower housing member 16 includes a connecting member 27 extending between base 18 of lower housing member 16 and inner wall 24 of upper housing member 14. The connecting member 27 is fixed to the inner wall 24 using screws 25.

The upper case member 14 defines a projection 29, the projection 29 defining a wall extending around a top surface of the upper case member 14. The edge of the top bracket 8 defines a rim 31, the rim 31 being dimensioned to extend around the outside of the protrusion 29. The combination of the protrusion 29 and the rim 31 prevents dirt and dust from entering the device 2.

The apparatus 2 includes a motor 30, the motor 30 including a stator 32 and a rotor 34. The stator 32 is a fixed component of the motor 30 and is disposed substantially within the lower chamber 26. The rotor 34 is a rotating component of the motor 30 and extends from the stator 32 through an aperture 36 in the inner wall 24 and into the upper chamber 28. The motor further includes a motor plate 38 coupled to the stator 32. The inventors have found that it is advantageous to provide a damping layer (not shown) between the stator 32 and the motor plate 38 to dampen vibrations. For example, in some embodiments, the inventors have used neoprene rubber mats as the damping layer. This dampens vibrations and allows flexibility and resistance to stirring movements. A motor plate 38 is disposed in the upper chamber 28 and extends through the aperture 36. A motor plate 38 is mounted to the inner wall 24 by screws 40 to couple the stator 32 to the upper case member 14. A cam 42 is mounted on the rotor 34 and is disposed in the upper chamber 28. The cam 42 defines a cylindrical shape, and the rotor 34 is mounted 1.25mm from the center of the cam 42.

As shown in fig. 2 and 3, a bottom bracket 44 is disposed in the upper chamber 28 and mounted to the top bracket 8 by screws 46. The inventors have found that the provision of a gasket material (not shown) between the top bracket 8 and the bottom bracket 44 reduces the noise generated by the apparatus 2. For example, the pad may comprise cotton wool.

A bearing 48 disposed between the bottom bracket 44 and the cam 42 couples these components together while minimizing friction due to rotation of the cam 42. Fig. 4 shows how the top bracket 8 is placed relative to the motor 30, with the tank 6 and bottom bracket 44 removed for clarity.

As shown in fig. 3, the bottom bracket 44 includes two projections 49. Tabs 49 are provided on opposite sides of the bottom bracket 44 and extend from the bottom surface of the bottom bracket 44 into corresponding circular apertures 51 in the motor plate 38. The hole 51 is large enough to allow the bottom bracket 44 to move in a circular motion as the cam 42 rotates, but prevents the bottom bracket 44 from rotating.

As mentioned above, the top bracket 8 defines a recess 12, the recess 12 being dimensioned to receive the container 4 therein. As shown in fig. 5, the top bracket 8 includes a lock 50, and the lock 50 includes a spring 52 and a contact member 54. The spring 52 is disposed parallel to the horizontal plane and is configured to bias the contact member 54 away from a front side 56 of the recess 12. Thus, when the container 4 is disposed in the recess 12, the spring 52 causes the contact member 54 to bias the container 4 against the rear side 58 of the recess 12. This holds the container 4 in place until the user applies sufficient force to the container 4 to remove the container 4.

The rear side 58 of the groove 12 defines an angle of 20.7 deg. from the vertical. Thus, placing the container 4 against the rear side 58 of the recess forms the base 60 of the container 4 at an angle of 20.7 ° to the horizontal. When the container 4 is inserted into the recess 12 at this angle, the base 60 will push the contact member 54 towards the front side 56 of the recess 12 until the base 60 of the container 4 has passed the contact member 54. At this point, the contact members 54 will exert a force on the sides 62 of the container 4, biasing the container 4 against the rear side 58 of the recess 12 and holding the container 4 in place, as explained above. In order to ensure maximum contact with the side 62 of the container 4, the contact side 64 of the contact member 54 also forms an angle of 20.7 ° with the vertical.

The device 2 includes an electrical adapter 66 and a power switch that allows the device to be attached to a power source. The adapter 66 is electrically connected (not shown) to a Printed Circuit Board (PCB)68, which PCB 68 is electrically connected to the motor 30. Thus, when the device 2 is connected to a power source and turned on, the motor 30 will rotate the cam 42, thereby moving the bottom bracket 44 and the top bracket 8 in a circular manner. The inventors have found that configuring motor 30 to operate at a speed of 300 Revolutions Per Minute (RPM) will provide desirable agitation to reduce the accumulation of PRP stored in container 4 disposed in recess 12.

Example 2: optional and/or additional features

The device 2 described in example 2 may include some additional features. In particular, the device 2 may comprise a battery disposed in the lower chamber 26. The battery will be configured to drive the device 2 when the device 2 is not attached to a power source. This may be particularly useful for people using PRP at home. For example, device 2 may be carried around when they obtain their PRP sample from a clinician. Once the container 4 containing their PRP is received, they can place it in the apparatus and ship it home. Since the PRP container will be placed in the device 2 and agitated once received, it does not matter if the person takes some time between receiving the sample and going home.

The device may also include a temperature control element. This may include a temperature sensor configured to sense temperature, a heater and/or a cooler configured to maintain the temperature of the device between 20 ℃ and 24 ℃. The temperature sensor may be exposed towards the base of the recess 12. The inventors have found that this temperature is ideal for storing PRP.

Further, rather than defining one recess 12, the top bracket 8 may be configured to define a plurality of recesses to allow the apparatus to store a plurality of containers 4. For example, fig. 7a shows a device 2 'configured to store four containers 4, fig. 7b shows a device 2 ″ configured to store eight containers 4, and fig. 7c shows a device 2 "' configured to store fifteen containers. This may be particularly useful for users who may want to apply PRP multiple times, for example in cosmetic applications. Each container 4 may include a sufficient amount for one application to ensure that the user receives the correct amount of PRP for each application.

Fig. 8 shows a further device 2'. In this apparatus, the top tray 8 defines fourteen recesses 12, allowing the apparatus to store fourteen containers 4. This may therefore provide a container of sufficient individual PRP to allow a user to apply an activated PRP to their skin twice a day a week. In this embodiment, the lid 10 is configured to be reversibly mounted over the top bracket 8 and the top portion of the case 6.