阅读说明：本技术 一种高纯度培养干细胞的培养装置及其培养方法 (Culture device and culture method for culturing stem cells with high purity ) 是由 黎鸠鸠 唐闪光 于 2021-09-18 设计创作，主要内容包括：本发明属于干细胞培养技术领域,尤其为一种高纯度培养干细胞的培养装置,包括培养箱,所述培养箱的背面安装有驱动气缸,所述驱动气缸的输出端连接有移动板,所述移动板可通过驱动气缸在培养箱的内部做水平向前或水平向后的直线运动,所述移动板的顶部连接有用于对培养瓶限位的置物组件。本发明过通风组件中的鼓风机在工作时,可实现对培养箱的内部进行换气,为了保证培养箱内部的无菌环境,将杀菌灯沿置物组件对培养瓶限位的位置做圆周直线运动,这样能够保证杀菌灯同时对培养箱的内部和培养瓶进行高效杀菌,使得培养瓶中的干细胞,可以在无菌的环境中进行培养,降低了干细胞死亡的几率和保证了干细胞培养的效果。(The invention belongs to the technical field of stem cell culture, and particularly relates to a culture device for culturing stem cells with high purity. The sterilizing lamp performs circular linear motion along the position where the storage component limits the culture bottle so as to ensure that the sterilizing lamp can perform efficient sterilization on the inside of the culture box and the culture bottle at the same time, so that stem cells in the culture bottle can be cultured in the sterile environment, the death probability of the stem cells is reduced, and the culture effect of the stem cells is ensured.)

1. A culture apparatus for culturing stem cells with high purity, comprising an incubator (1), characterized in that: the back side of the incubator (1) is provided with a driving cylinder (2), the output end of the driving cylinder (2) is connected with a movable plate (3), the movable plate (3) can move forward or backward in the incubator (1) through the driving cylinder (2), the top of the movable plate (3) is connected with an object placing component (4) for limiting the culture bottle, a sterilization mechanism (5) is arranged above the object placing component (4), the sterilization mechanism (5) comprises a servo motor (501), the servo motor (501) is arranged at the bottom of the movable plate (3), the output end of the servo motor (501) is connected with a rotating rod (502), the servo motor (501) and the rotating rod (502) are both located at the center of the movable plate (3), the top end of the rotating rod (502) is connected with a disc (503), the surface of the disc (503) is connected with an L-shaped support rod (504), the other end of L type bracing piece (504) is connected with mounting panel (505) of circular form, the bottom of mounting panel (505) is dismantled and is equipped with bactericidal lamp (506), the top of incubator (1) is equipped with ventilation assembly (6) with incubator (1) inside intercommunication.

2. The culture apparatus for culturing stem cells with high purity according to claim 1, wherein: the article placing component (4) comprises a circular limiting ring (401), the circular limiting ring (401) is connected with the top of the moving plate (3), the inner wall of the circular limiting ring (401) is connected with a fixed block (402), the bottom of the fixed block (402) is contacted with the top of the moving plate (3), the surface of the fixed block (402) is provided with a first arc-shaped groove (403), two mounting grooves (404) are symmetrically arranged on two sides of the fixed block (402), the inner wall of the mounting groove (404) is connected with a guide rod (405), the surface of the guide rod (405) is sleeved with a spring (406), a T-shaped sliding block (407) is arranged on the surface of the guide rod (405) in a sliding manner, a connecting rod (408) is connected to the surface of the T-shaped sliding block (407), the other end of the connecting rod (408) is connected with a concave block (409), and a second arc-shaped groove (410) is formed in one surface, corresponding to the first arc-shaped groove (403), of the concave block (409).

3. The culture apparatus for culturing stem cells with high purity according to claim 2, wherein: the concave block (409) corresponds to the center of the fixing block (402), the height of the concave block (409) is one fourth of the height of the fixing block (402), and the germicidal lamp (506) corresponds to the upper part between the concave block (409) and the fixing block (402).

4. The culture apparatus for culturing stem cells with high purity according to claim 1, wherein: ventilation assembly (6) include spacing groove (601), the inner diapire of spacing groove (601) is equipped with vent (602) with incubator (1) inside intercommunication, the inner wall contact of spacing groove (601) has filter screen (603) with spacing groove (601) looks adaptation, the top of filter screen (603) is equipped with communicating solid fixed ring (604) of spacing groove (601), air-blower (605) are installed at the top of solid fixed ring (604), the surface connection of solid fixed ring (604) has connecting plate (606), the top at incubator (1) is connected through the pivot in connecting plate (606).

5. The culture apparatus for culturing stem cells with high purity according to claim 4, wherein: the bottom of connecting plate (606) and solid fixed ring (604) all contacts with the top of incubator (1) respectively, the top of connecting plate (606) and the top of incubator (1) are equipped with same screw hole (607) respectively, the inner wall threaded connection of screw hole (607) has lead screw (608).

6. The culture apparatus for culturing stem cells with high purity according to claim 1, wherein: the both sides of movable plate (3) are equipped with concave groove (7) of two symmetries, the inner wall contact of concave groove (7) has limiting plate (8), the opposite side of limiting plate (8) is connected on the inside wall of incubator (1), the width of concave groove (7) inner wall is limiting plate (8) surface width's half, there is chamber door (9) on the surface of incubator (1) through hinge connection.

7. The method for culturing a culture apparatus for culturing stem cells with high purity according to any one of claims 1 to 6, wherein: the method comprises the following steps:

step A, when the moving plate (3) is pushed by the driving cylinder (2) to do horizontal forward linear motion towards the interior of the incubator (1), the concave grooves (7) on the two sides of the moving plate (3) stick to the surface of the limiting plate (8) and extend out of the incubator (1), when the moving plate (3) is positioned at the outermost part of the incubator (1), the incubator can be placed between the first arc-shaped groove (403) and the second arc-shaped groove (410) in the storage component (4) arranged at the top of the moving plate (3), when the surface of the incubator extrudes the concave block (409), the connecting rod (408) connected with the surface of the concave block (409) drives the T-shaped sliding block (407) to slide on the surface of the guide rod (405) to extrude the spring (406), and the second arc-shaped groove (410) in the concave block (409) can be limited between the concave block (402) and the concave block (409) under the elastic force of the spring (406), then, when the driving cylinder (2) drives the movable plate (3) to do horizontal backward linear motion towards the inside of the incubator (1), the movable plate (3) stretches the culture bottle limited in the storage component (4) into the incubator (1) for culture, and in order to ensure that stem cells in the culture bottle are cultured in a sterile environment, the inside of the incubator (1) can be ventilated and sterilized through the ventilation component (6) and the sterilization mechanism (5) respectively;

step B, when the inside of the incubator 1 is ventilated, only the blower 605 is required to be driven, the blower 605 can discharge the gas inside the incubator 1, and the external gas enters the inside of the incubator 1 after being filtered by the filter screen 603, so that the inside of the incubator 1 can be ventilated, when the sterilization mechanism 5 sterilizes the inside of the incubator 1, because the sterilizing lamp (506) is positioned above the culture bottle and is connected with the disc (503) through the mounting plate (505) and the L-shaped support rod (504), when the rotating rod (502) and the disc (503) are driven by the servo motor (501) to rotate, the sterilizing lamp (506) can do circular linear motion along the upper part of the circular limit ring (401), and the plurality of culture bottles are limited on the inner wall of the circular limit ring (401) in a circular array manner, so that the sterilizing lamp (506) can be guaranteed to do circular motion, can simultaneously sterilize the inside of the incubator (1) and the culture bottle.

Technical Field

The invention relates to the technical field of stem cell culture, in particular to a culture device for culturing stem cells with high purity and a culture method thereof.

Background

The definition of stem cells has been revised over the years and defined at different levels, and most biologists and physicians now consider stem cells to be from a class of cells in the embryonic or adult body that have the ability to self-renew and proliferate and differentiate without limitation under certain conditions, and that are capable of producing daughter cells with the same phenotype and genotype as themselves, as well as specialized cells that make up body tissues and organs, and that are capable of differentiating into progenitor cells.

The stem cells are cells with unlimited or immortal self-renewal capacity and can produce highly differentiated progeny cells, but the existing culture device for culturing the stem cells with high purity has the defect of inconvenient sterilization, the stem cells are difficult to culture in a good sterile environment, the stem cells are extremely easy to be infected by bacteria in the culture process, the death probability of the stem cells is improved, the culture effect of the stem cells is influenced, and the use is inconvenient. Therefore, the invention provides a culture device for culturing stem cells with high purity and a culture method thereof.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a culture device for culturing stem cells with high purity and a culture method thereof, which solve the problems that the existing culture device for culturing stem cells with high purity has the defect of inconvenient sterilization, is very easy to cause the stem cells to be infected by bacteria in the culture process, and improves the death probability of the stem cells.

(II) technical scheme

The invention specifically adopts the following technical scheme for realizing the purpose:

a culture device for culturing stem cells with high purity comprises an incubator, wherein a driving cylinder is installed on the back face of the incubator, the output end of the driving cylinder is connected with a movable plate, the movable plate can make linear motion forwards or backwards horizontally in the incubator through the driving cylinder, the top of the movable plate is connected with a storage component for limiting a culture bottle, a sterilization mechanism is arranged above the storage component and comprises a servo motor, the servo motor is installed at the bottom of the movable plate, the output end of the servo motor is connected with a rotating rod, the servo motor and the rotating rod are both located at the center of the movable plate, the top end of the rotating rod is connected with a disc, the surface of the disc is connected with an L-shaped supporting rod, the other end of the L-shaped supporting rod is connected with a circular mounting plate, and a sterilization lamp is disassembled and arranged at the bottom of the mounting plate, the top of incubator is equipped with the ventilation subassembly with the inside intercommunication of incubator.

Further, it includes circular spacing ring to put the thing subassembly, circular spacing ring connects on the top of movable plate, circular spacing ring's inner wall connection has the fixed block, the bottom of fixed block contacts with the top of movable plate, the surface of fixed block is equipped with first arc wall, the bilateral symmetry of fixed block is equipped with two mounting grooves, the inner wall connection of mounting groove has the guide bar, the spring has been cup jointed on the surface of guide bar, the surface slip of guide bar is equipped with T type slider, the surface of T type slider is connected with the connecting rod, the other end of connecting rod is connected with concave type piece, concave type piece is equipped with the second arc wall with the one side that first arc wall corresponds.

Furthermore, the concave block corresponds to the center of the fixing block, the height of the concave block is one fourth of the height of the fixing block, and the germicidal lamp corresponds to the position above the position between the concave block and the fixing block.

Further, the ventilation subassembly includes the spacing groove, the inner diapire of spacing groove is equipped with the vent with the inside intercommunication of incubator, the inner wall contact of spacing groove have with the filter screen of spacing groove looks adaptation, the top of filter screen is equipped with the communicating solid fixed ring of spacing groove, the air-blower is installed at solid fixed ring's top, gu fixed ring's surface is connected with the connecting plate, the top at the incubator is connected through the pivot to the connecting plate.

Further, the bottom of connecting plate and solid fixed ring all contacts with the top of incubator respectively, the top of connecting plate and the top of incubator are equipped with the same screw hole respectively, the inner wall threaded connection of screw hole has the lead screw.

Further, the both sides of movable plate are equipped with the concave groove of two symmetries, the inner wall contact of concave groove has the limiting plate, the opposite side of limiting plate is connected on the inside wall of incubator, the width of concave groove inner wall is the half of limiting plate surface width, there is the chamber door on the surface of incubator through hinge connection.

The invention also provides a culture method of the culture device for culturing the stem cells with high purity, which comprises the following steps:

step A, when the moving plate is pushed by the driving cylinder to do horizontal forward linear motion towards the inside of the incubator, the concave grooves at two sides of the moving plate extend out of the incubator along the surface of the limiting plate, when the moving plate is positioned at the outermost part of the incubator, the culture bottle can be placed between the first arc-shaped groove and the second arc-shaped groove in the storage component arranged at the top of the moving plate, when the surface of the culture bottle extrudes the concave blocks, the connecting rods connected with the surface of the concave blocks can drive the T-shaped sliding blocks to slide on the surface of the guide rods to extrude the springs, at the moment, the second arc-shaped groove in the concave blocks can be limited between the fixed blocks and the concave blocks under the elastic force of the springs, then the moving plate is driven by the driving cylinder to do horizontal backward linear motion towards the inside of the incubator, the culture bottle limited in the storage component extends into the inside of the incubator to be cultured, in order to ensure that the stem cells in the culture bottle are cultured in a sterile environment, the interior of the culture box can be ventilated and sterilized through the ventilation assembly and the sterilization mechanism respectively;

step B, when taking a breath to the inside of incubator, only need the drive through the air-blower, the air-blower can discharge the inside gas of incubator, and outside gas gets into the inside of incubator after the filter screen filters, and then can realize taking a breath to the inside of incubator, when the mechanism of disinfecting is disinfected to the inside of incubator, because the bactericidal lamp is located the top of blake bottle, and be connected with the disc through mounting panel and L type bracing piece, so when servo motor drives bull stick and disc rotation, the circumference linear motion can be made along the top of circular spacing ring to the bactericidal lamp, and a plurality of blake bottles are the inner wall department that the circumference array is spacing at circular spacing ring again, can guarantee like this that the bactericidal lamp is when making circular motion, can carry out germicidal treatment to the inside of incubator and blake bottle simultaneously.

(III) advantageous effects

Compared with the prior art, the invention provides a culture device for culturing stem cells with high purity and a culture method thereof, and the culture device has the following beneficial effects:

according to the invention, the inner part of the incubator can be ventilated when the blower in the ventilation assembly works, and the sterilizing lamp circularly and linearly moves along the position where the storage assembly limits the culture bottle in order to ensure the sterile environment in the incubator, so that the sterilizing lamp can be ensured to efficiently sterilize the inner part of the incubator and the culture bottle at the same time, stem cells in the culture bottle can be cultured in the sterile environment, the death probability of the stem cells is reduced, and the culture effect of the stem cells is ensured.

Drawings

FIG. 1 is a schematic view of the internal structure of an incubator according to the present invention;

FIG. 2 is a sectional top view of the incubator of the present invention;

FIG. 3 is a schematic view of the connection structure of the sterilization mechanism and the movable plate according to the present invention;

FIG. 4 is a schematic view of a connecting structure of the placement module and the moving plate according to the present invention;

FIG. 5 is a schematic view of the connection structure of the fixing block and the concave block according to the present invention;

FIG. 6 is a schematic view of the connection structure of the ventilation assembly and the incubator according to the present invention;

fig. 7 is an exploded view of the retainer ring and blower of the present invention.

In the figure: 1. an incubator; 2. a driving cylinder; 3. moving the plate; 4. an article placing component; 401. a circular limiting ring; 402. a fixed block; 403. a first arc-shaped slot; 404. mounting grooves; 405. a guide bar; 406. a spring; 407. a T-shaped slider; 408. a connecting rod; 409. a concave block; 410. a second arc-shaped slot; 5. a sterilization mechanism; 501. a servo motor; 502. a rotating rod; 503. a disc; 504. an L-shaped support bar; 505. mounting a plate; 506. a germicidal lamp; 6. a ventilation assembly; 601. a limiting groove; 602. a vent; 603. a filter screen; 604. a fixing ring; 605. a blower; 606. a connecting plate; 607. a threaded hole; 608. a screw rod; 7. a concave groove; 8. a limiting plate; 9. and (4) a box door.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Examples

As shown in fig. 1-3, a culture apparatus for culturing stem cells with high purity according to an embodiment of the present invention includes an incubator 1, a driving cylinder 2 is installed on the back of the incubator 1, a moving plate 3 is connected to an output end of the driving cylinder 2, the moving plate 3 can make a linear motion horizontally forward or horizontally backward inside the incubator 1 through the driving cylinder 2, an object placing component 4 for limiting the position of the incubator is connected to the top of the moving plate 3, and a sterilization mechanism 5 is disposed above the object placing component 4, wherein the object placing component 4 can be used in conjunction with the sterilization mechanism 5, because the position for limiting the position of the incubator in a circular limiting ring 401 exactly corresponds to the position of a sterilization lamp 506 above, when the sterilization lamp 506 rotates above the circular limiting ring 401, the inside of the incubator 1 and the incubator can be sterilized at the same time, and the stem cells can be cultured in a sterile environment, the object placing component 4 can extend out of the incubator 1 through the moving plate 3, so that the culture bottle can be conveniently taken out or placed, and the sterilizing lamp 506 arranged above the circular limiting ring 401 can be cleaned or replaced, the sterilizing mechanism 5 comprises a servo motor 501, the servo motor 501 is arranged at the bottom of the moving plate 3, the output end of the servo motor 501 is connected with a rotating rod 502, the servo motor 501 and the rotating rod 502 are both positioned at the central position of the moving plate 3, the top end of the rotating rod 502 is connected with a disc 503, the surface of the disc 503 is connected with an L-shaped supporting rod 504, the other end of the L-shaped supporting rod 504 is connected with a circular mounting plate 505, the sterilizing lamp 506 is detachably arranged at the bottom of the mounting plate 505, the bottom of the mounting plate 505 is provided with a threaded rod which is matched with a threaded groove formed in the top of the sterilizing lamp 506, when the sterilizing lamp 506 is replaced, the sterilizing lamp 506 can be taken out from the surface of the bottom of the mounting plate 505 by only rotating the sterilizing lamp 506, and replaced, the top of the incubator 1 is provided with a ventilation assembly 6 communicating with the inside of the incubator 1.

As shown in fig. 4 and 5, in some embodiments, the placement component 4 includes a circular limiting ring 401, the circular limiting ring 401 is connected to the top of the moving plate 3, the inner wall of the circular limiting ring 401 is connected to a fixed block 402, the bottom of the fixed block 402 is in contact with the top of the moving plate 3, the surface of the fixed block 402 is provided with a first arc-shaped groove 403, two sides of the fixed block 402 are symmetrically provided with two installation grooves 404, the inner wall of the installation groove 404 is connected to a guide rod 405, the surface of the guide rod 405 is sleeved with a spring 406, the surface of the guide rod 405 is slidably provided with a T-shaped slider 407, the surface of the T-shaped slider 407 is connected to a connecting rod 408, the other end of the connecting rod 408 is connected to a concave block 409, one side of the concave block 409 corresponding to the first arc-shaped groove 403 is provided with a second arc-shaped groove 410, wherein the placement component 4 is mainly used for placing a culture bottle in a limiting manner, and the culture bottle is limited in the position of the inner wall of the circular limiting ring 401, the sterilizing lamp 506 is designed to do circular linear motion along the inside of the incubator 1, so that the interiors of the culture bottle and the incubator 1 can be sterilized respectively with high efficiency, and the condition that the dry and fine culture can be performed in a good sterile environment is guaranteed.

As shown in fig. 5, in some embodiments, the concave block 409 corresponds to the center of the fixing block 402, the height of the concave block 409 is one fourth of the height of the fixing block 402, the germicidal lamp 506 corresponds to the upper portion between the concave block 409 and the fixing block 402, and the germicidal lamp 506 is disposed between the concave block 409 and the fixing block 402, because the germicidal lamp is located between the fixing block 402 and the concave block 409 during the culture bottle, and the culture bottles are arranged on the inner wall of the circular position-limiting ring 401 according to a circumferential array, when the germicidal lamp 506 moves linearly along the upper portion of the circular position-limiting ring 401 in a circumferential manner, the germicidal lamp 506 can sterilize both the culture bottles and the inside of the incubator 1, and can ensure that the stem cells are cultured in a sterile environment.

As shown in FIG. 6, in some embodiments, the ventilation assembly 6 includes a limiting groove 601, the inner bottom wall of the limiting groove 601 is provided with a vent 602 communicating with the inside of the incubator 1, the inner wall of the limiting groove 601 contacts a filter screen 603 matching with the limiting groove 601, a fixing ring 604 communicating with the limiting groove 601 is arranged above the filter screen 603, the top of the fixing ring 604 is provided with a blower 605, when the blower 605 is in operation, the air inside the incubator 1 can be exhausted, and the outside air can enter the inside of the incubator 1 from the vent 602 after being filtered by the filter screen 603, so as to ventilate the inside of the incubator 1, and the inside of the incubator 1 is provided with a germicidal lamp 506, when the germicidal lamp 506 makes a circular linear motion inside of the incubator 1, the inside of the incubator 1 can be sterilized, so that the dry cells in the culture flask can be cultured in a good environment, the probability of stem cell death is reduced, the effect of stem cell culture is guaranteed, the surface of the fixing ring 604 is connected with the connecting plate 606, and the connecting plate 606 is connected to the top of the incubator 1 through a rotating shaft.

As shown in fig. 6 and 7, in some embodiments, the connection plate 606 and the bottom of the fixing ring 604 are respectively in contact with the top of the incubator 1, and the connection plate 606 is in circular linear motion on the surface of the incubator 1 through a rotation shaft, so that in order to facilitate cleaning of the filter screen 603 in the limiting groove 601 later, the screw 608 is only required to be rotated inside the threaded hole 607, and when the screw 608 is disengaged from the threaded hole 607, the connection plate 606 is rotated on the surface of the incubator 1 through the rotation shaft, when the fixing ring 604 drives the blower 605 to be disengaged from the limiting groove 601, the filter screen 603 can be taken out from the inner wall of the limiting groove 601 for replacement and cleaning, and then after the filter screen 603 is replaced or cleaned, the fixing ring 604 is rotated to the corresponding limiting groove 601, and rotated to the inside the threaded hole 607 through the screw 608, the blower 605 on the top of the fixing ring 604 can be positioned at the corresponding limiting groove 601, the top of the connecting plate 606 and the top of the incubator 1 are respectively provided with the same threaded hole 607, and the inner wall of the threaded hole 607 is in threaded connection with a screw rod 608.

As shown in fig. 1 and 3, in some embodiments, two symmetrical concave grooves 7 are formed on two sides of the moving plate 3, and the inner walls of the concave grooves 7 contact with a limiting plate 8, wherein the limiting plate 8 not only can support the moving plate 3, but also can be used for matching with the concave grooves 7 formed in the moving plate 3 and can slide along the surface of the limiting plate 8, when the moving plate 3 extends to the outside of the incubator 1, the incubator can be conveniently placed in the storage assembly 4 for limiting, and the germicidal lamp 506 is arranged above the storage assembly 4, and when the moving plate 3 extends to the outside of the incubator 1, the surface dirt of the germicidal lamp 506 can be cleaned, so that the germicidal efficiency of the germicidal lamp 506 can be ensured, the other side of the limiting plate 8 is connected to the inner side wall of the incubator 1, the width of the inner wall of the concave groove 7 is half of the width of the surface of the limiting plate 8, the surface of the incubator 1 is connected with a chamber door 9 through a hinge.

The invention also provides a culture method of the culture device for culturing the stem cells with high purity, which comprises the following steps:

step A, when the moving plate 3 is pushed by the driving cylinder 2 to do horizontal forward linear motion towards the inside of the incubator 1, the concave grooves 7 at the two sides of the moving plate 3 extend towards the outside of the incubator 1 along the surface of the limiting plate 8, when the moving plate 3 is positioned at the outermost part of the incubator 1, the culture bottle can be placed between the first arc-shaped groove 403 and the second arc-shaped groove 410 in the storage component 4 arranged at the top of the moving plate 3, when the surface of the culture bottle extrudes the concave block 409, the connecting rod 408 connected with the surface of the concave block 409 drives the T-shaped sliding block 407 to slide on the surface of the guide rod 405 to extrude the spring 406, at the moment, the second arc-shaped groove 410 in the concave block 409 is under the elastic force of the spring 406, so that the culture bottle can be limited between the fixed block 402 and the concave block 409, and then the driving cylinder 2 drives the moving plate 3 to do horizontal backward linear motion towards the inside of the incubator 1, the moving plate 3 extends the culture bottle limited in the storage component 4 into the culture box 1 for culture, and in order to ensure that stem cells in the culture bottle are cultured in a sterile environment, the interior of the culture box 1 can be ventilated and sterilized through the ventilation component 6 and the sterilization mechanism 5 respectively;

step B, when ventilating the inside of the incubator 1, the blower 605 is driven to discharge the gas inside the incubator 1, and the outside gas is filtered by the filter screen 603 and then enters the inside of the incubator 1, further, the inside of the incubator 1 can be ventilated, and when the sterilization means 5 sterilizes the inside of the incubator 1, since the germicidal lamp 506 is located above the culture flask, and is connected to the disk 503 through the mounting plate 505 and the L-shaped support rod 504, therefore, when the servo motor 501 drives the rotating rod 502 and the disc 503 to rotate, the germicidal lamp 506 will make a circular linear motion along the upper portion of the circular limiting ring 401, and a plurality of culture bottles are limited on the inner wall of the circular limiting ring 401 in a circular array manner, so that the sterilizing lamp 506 can simultaneously sterilize the inside of the incubator 1 and the culture bottles when doing circular motion.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.