阅读说明：本技术 一种生物组织冷冻研磨装置 (Biological tissue cryo-grinding device ) 是由 连博琳 黄云鹏 刘世薇 张健 余春梅 陈艳红 刘国元 钟非 赵媛媛 柯勇超 于 2021-08-06 设计创作，主要内容包括：本发明公开了一种生物组织冷冻研磨装置,包括底座和上盖,底座内设有研钵容纳槽,研钵容纳槽与底座壳体之间设有液氮腔室,研钵容纳槽内放置有研钵,研钵的横截面为圆环形。上盖内设有电机以及连接所述电机的传动机构,在上盖底部竖直设置三根研磨棒,当上盖与底座盖合时,第一研磨棒由传动机构驱动在研钵内绕中央柱体做逆时针圆周转动,其余两根研磨棒中心对称布设在研钵内并在研钵内沿侧壁面做顺时针圆周转动。中心对称的研磨棒的侧面由相对的一组弧形面以及相对的一组斜面形成。本装置特别适用于量大的冷冻样本进行自动化粗研磨,操作简便,研磨时不冻手、泼溅,省时省力又安全。整个装置结构简单,制造成本相对较低。(The invention discloses a biological tissue freezing and grinding device which comprises a base and an upper cover, wherein a mortar accommodating groove is arranged in the base, a liquid nitrogen chamber is arranged between the mortar accommodating groove and a base shell, a mortar is placed in the mortar accommodating groove, and the cross section of the mortar is circular. Be equipped with the motor in the upper cover and connect the drive mechanism of motor, at the vertical three grinding rods that set up in upper cover bottom, when the upper cover was fashionable with the base lid, first grinding rod was done anticlockwise circumference by the drive mechanism drive around central cylinder in the mortar and is rotated, and clockwise circumference is done along the lateral wall face in the mortar to other two grinding rod centrosymmetries are laid in the mortar and in the mortar. The sides of the centrosymmetric grinding rod are formed by a set of arc-shaped surfaces and a set of inclined surfaces. The device is particularly suitable for automatic rough grinding of large-volume frozen samples, is simple and convenient to operate, does not freeze hands or splash during grinding, and is time-saving, labor-saving and safe. The whole device has simple structure and relatively low manufacturing cost.)

1. A biological tissue freezing and grinding device is characterized by comprising a base and an upper cover, wherein a mortar accommodating groove is formed in the base, a liquid nitrogen chamber is formed between the mortar accommodating groove and a base shell, a mortar is placed in the mortar accommodating groove, the cross section of the mortar is circular, and a central upright column is arranged in the mortar; the upper cover is internally provided with a motor and a transmission mechanism connected with the motor, the bottom of the upper cover is vertically provided with three grinding rods, when the upper cover is covered with the base, the first grinding rod is driven by the transmission mechanism to rotate around the central cylinder in a counterclockwise circular manner in the mortar, and the second grinding rod and the third grinding rod are symmetrically distributed in the mortar in a central symmetry manner and driven by the transmission mechanism to rotate in a clockwise circular manner along the side wall surface in the mortar; wherein, the side of second grinding rod and third grinding rod is formed by a set of relative arcwall face and a set of relative inclined plane, and the radian of the arcwall face of pressing close to the mortar inside wall face is unanimous with the mortar radian, and the arc length is greater than another relative arcwall face, and the protruding direction of two arcwall faces is unanimous.

2. The biological tissue cryo-grinding apparatus of claim 1, wherein the mortar receiving groove is made of copper and is wrapped with a rubber layer outside the base housing.

3. The biological tissue cryo-grinding apparatus according to claim 1, wherein the top of the mortar-receiving groove is provided with a groove for facilitating removal of the mortar.

4. The biological tissue cryo-grinding apparatus according to claim 1, wherein the liquid nitrogen inlet of the liquid nitrogen chamber is located at the top of the base, and a plug of the liquid nitrogen inlet is correspondingly disposed on the bottom surface of the base.

5. The biological tissue cryo-grinding apparatus according to claim 1, wherein a touch pad is disposed on a top of the upper cover, and the touch pad is connected to a driving controller of the motor.

Technical Field

The invention relates to a liquid nitrogen freezing type grinding device for biological tissues.

Background

In biological related scientific researches, the grinding of frozen samples is often needed, the traditional manual grinding is time-consuming and labor-consuming, liquid nitrogen is volatile at normal temperature, so that the liquid nitrogen is not fully used and is wasted, and the manual grinding of a large number of samples of different types is very laborious. The automatic cryogenic grinding machines available on the market are suitable for the case of small sample size, the equipment price is very expensive although the grinding is fine, and in addition, the steel balls used in the products have great trouble in cleaning.

Disclosure of Invention

The purpose of the invention is as follows: to the prior art, a biological tissue freezing and grinding device is provided, which can perform automatic rough grinding on batch biological tissues.

The technical scheme is as follows: a biological tissue freezing and grinding device comprises a base and an upper cover, wherein a mortar accommodating groove is formed in the base, a liquid nitrogen chamber is arranged between the mortar accommodating groove and a base shell, a mortar is placed in the mortar accommodating groove, the cross section of the mortar is circular, and a central upright column is arranged in the mortar; the upper cover is internally provided with a motor and a transmission mechanism connected with the motor, the bottom of the upper cover is vertically provided with three grinding rods, when the upper cover is covered with the base, the first grinding rod is driven by the transmission mechanism to rotate around the central cylinder in a counterclockwise circular manner in the mortar, and the second grinding rod and the third grinding rod are symmetrically distributed in the mortar in a central symmetry manner and driven by the transmission mechanism to rotate in a clockwise circular manner along the side wall surface in the mortar; wherein, the side of second grinding rod and third grinding rod is formed by a set of relative arcwall face and a set of relative inclined plane, and the radian of the arcwall face of pressing close to the mortar inside wall face is unanimous with the mortar radian, and the arc length is greater than another relative arcwall face, and the protruding direction of two arcwall faces is unanimous.

Further, the mortar accommodating groove is made of copper materials, and a rubber layer is wrapped outside the base shell.

Furthermore, the top of the mortar accommodating groove is provided with a groove which is convenient for taking out the mortar.

Furthermore, a liquid nitrogen inlet of the liquid nitrogen chamber is positioned at the top of the base, and a plug of the liquid nitrogen inlet is correspondingly arranged on the bottom surface of the base.

Furthermore, a touch control plate is arranged at the top of the upper cover and connected with a driving controller of the motor.

Has the advantages that: the biological tissue freezing and grinding device is particularly suitable for automatically and roughly grinding large-quantity frozen multi-samples, is simple and convenient to operate, does not freeze hands or splash during grinding, and is time-saving, labor-saving and safe. The equipment can effectively reduce the consumption of liquid nitrogen because of the closed chamber for storing the liquid nitrogen, and simultaneously, the equipment can start to work only by installing the equipment according to steps and pouring the liquid nitrogen. The whole device has simple structure and relatively low manufacturing cost.

Drawings

FIG. 1 is a schematic top view of the base of the present apparatus;

FIG. 2 is a schematic cross-sectional view of the base of the present apparatus;

FIG. 3 is a diagram showing the positional relationship between the grinding rod and the mortar, wherein the dotted line represents the movement locus of the center of the inner grinding rod;

FIG. 4 is a sectional view showing the positional relationship between the grinding rod and the mortar;

FIG. 5 is a schematic cross-sectional view of an outer grinding rod.

Detailed Description

The invention is further explained below with reference to the drawings.

As shown in fig. 1 to 5, a biological tissue freezing and grinding device comprises a base and an upper cover, wherein a mortar holding tank 1 is arranged in the base, a liquid nitrogen chamber 2 is arranged between the mortar holding tank 1 and a base shell, a mortar is arranged in the mortar holding tank 1, the cross section of the mortar is circular, and a central upright post 4-1 is arranged in the mortar.

The upper cover is internally provided with a motor and a transmission mechanism connected with the motor, the bottom of the upper cover is vertically provided with three grinding rods, when the upper cover is covered with the base, one grinding rod 3-1 is driven by the transmission mechanism to rotate around a central cylinder 4-1 in an anticlockwise circumferential manner in the mortar 4, the other two grinding rods 3-2 are symmetrically distributed in the mortar 4 in a center manner and driven by the transmission mechanism to rotate in a clockwise circumferential manner along the side wall surface in the mortar, and the grinding rods 3-1 are positioned at the opposite inner sides of the grinding rods 3-2.

Wherein, the side surfaces of the two grinding rods 3-2 are formed by a group of opposite arc surfaces and a group of opposite inclined surfaces, the radian of the arc surface 3-2-1 close to the inner side wall surface of the mortar 4 is consistent with that of the mortar 4, the arc length is longer than that of the other opposite arc surface 3-2-2, and the protruding directions of the two arc surfaces are consistent. Two grinding rods 3-2 symmetrically arranged in the center are closely adjacent to the running track of the grinding rod 3-1 which rotates around the central cylinder 4-1, and a slight gap is left between the grinding rods 3-1 and the grinding rods 3-2 when the grinding rods 3-1 run to the position opposite to the grinding rods 3-2.

Mortar holding tank 1 is copper material to outside the base casing parcel rubber layer 5, play heat preservation and absorbing effect. The top of the mortar holding tank 1 is relatively provided with a groove 6 convenient for taking out a mortar, so that the grinding is convenient for sampling and cleaning. The liquid nitrogen inlet 2-1 of the liquid nitrogen chamber is positioned at the top of the base, and the bottom surface of the upper cover is correspondingly provided with a plug of a liquid nitrogen inlet. The top of the upper cover is provided with a touch pad which is connected with a driving controller of the motor.

When the device is used, a mortar 4 is placed in the mortar accommodating groove 1, liquid nitrogen is injected into the liquid nitrogen chamber 2, biological tissues to be ground are added into the mortar 4, the cover is closed, then the motor starts to work, and effective grinding spaces are formed between the grinding rod 3-1 and the central cylinder 4-1, between the grinding rod 3-1 and the arc-shaped surface 3-2-2 of the grinding rod 3-2 and between the arc-shaped surface 3-2-1 of the grinding rod 3-2 and the inner side wall surface of the mortar 4. When the grinding rod 3-1 is moved to a position opposite to the grinding rod 3-2, the rotation tendency of the two is opposite, so that the biological tissue between the two is sufficiently ground.

Meanwhile, the inclined plane 3-2-3 which is opposite to the biological tissue when the grinding rod 3-2 rotates clockwise can lead the sample to gather towards the inner track in the rotating and grinding process. The other bevel 3-2-4 is used to reduce the overall weight of the grinding rod.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.