阅读说明：本技术 一种干细胞培养容器 (Stem cell culture container ) 是由 曾峰 曾湘 杨金芳 李想 于 2021-09-07 设计创作，主要内容包括：本发明属于培养容器技术领域,公开一种干细胞培养容器,包括底座、培养容器、培养盘、容器盖、药液混合预热筒、空气循环泵和培养导入装置底座上安装有培养容器,立柱上安装有与培养容器相连接的电动伸缩杆,容器盖顶部安装有药液混合预热筒,药液混合预热筒内壁设置有水浴加热腔；容器盖顶部还安装有空气循环泵；本发明在容器盖上安装药液混合预热筒进行培养液预热混合,空气换气泵对培养容器内换气,培养容器内的温湿度传感器与控制器面板电性连接,进行分类控制；通过培养盘和培养导入装置的连接设置为干细胞生长繁殖提供适量的水份和培养液,底部的产生气体通过防水透气膜进入透气孔中被排放,给干细胞生长繁殖提供了良好的环境。(The invention belongs to the technical field of culture containers, and discloses a stem cell culture container which comprises a base, a culture container, a culture tray, a container cover, a liquid medicine mixing preheating cylinder, an air circulating pump and a culture introduction device, wherein the culture container is arranged on the base; the top of the container cover is also provided with an air circulating pump; according to the invention, a liquid medicine mixing preheating cylinder is arranged on a container cover to preheat and mix culture solution, an air exchange pump is used for exchanging air in a culture container, and a temperature and humidity sensor in the culture container is electrically connected with a controller panel to perform classification control; the connection of the culture tray and the culture leading-in device is used for providing a proper amount of water and culture solution for the growth and the propagation of the stem cells, and the generated gas at the bottom enters the air holes through the waterproof air-permeable membrane to be discharged, so that a good environment is provided for the growth and the propagation of the stem cells.)

1. A stem cell culture container is characterized by comprising a base (1), a culture container (2), a culture tray (3), a container cover (4), a liquid medicine mixing preheating cylinder (5), an air circulating pump (6) and a culture introduction device (9); a culture container (2) is installed on a base (1), a container cover (4) is hermetically arranged at the top of the culture container (2), stand columns (7) are installed at two ends of the base (1), an electric telescopic rod (8) connected with the culture container (2) is installed on each stand column (7), a liquid medicine mixing preheating cylinder (5) is installed at the top of the container cover (4), a water bath heating cavity (51) is arranged on the inner wall of the liquid medicine mixing preheating cylinder (5), and a stirring rod (55) is arranged inside the liquid medicine mixing preheating cylinder (5); an air circulating pump (6) is further installed at the top of the container cover (4), an air inlet end of the air circulating pump (6) is connected with an air inlet pipe (61) and a first air exchange pipe (62), and an air outlet end of the air circulating pump (6) is connected with the inside of the culture container (2) through a second air exchange pipe (63);

the bottom in the culture container (2) is provided with a culture tray (3), the culture tray (3) is provided with a culture lead-in device (9), and the middle in the culture container (2) is also provided with a bracket (21) for limiting the culture lead-in device (9).

2. The stem cell culture container according to claim 1, wherein a stirring motor (52), a first liquid injection port (53) and a second liquid injection port (54) are arranged at the top of the liquid medicine mixing preheating cylinder (5), the stirring motor (52) is in driving connection with a stirring rod (55), an electric heater (56) is arranged on one side of the liquid medicine mixing preheating cylinder (5), and an electric heating sheet electrically connected with the electric heater (56) is arranged in the water bath heating cavity (51); the bottom of the liquid medicine mixing preheating cylinder (5) is provided with a liquid outlet pipe (57) with an electromagnetic valve.

3. Stem cell culture vessel according to claim 1, wherein the first venting tube (62) connects the air inlet tube (61) and the culture vessel (2); the air inlet pipe (61) and the first scavenging pipe (62) are respectively provided with a first switch valve (64) and a second switch valve (65).

4. Stem cell culture vessel according to claim 1, wherein the culture tray (3) has a culture chamber (32) formed therein, the side surface and the bottom surface of the culture tray (3) are provided with air holes (36), the inner bottom surface and the inner surface of the culture tray (3) are both adhered with waterproof breathable films (33), and a water storage layer (34) is tightly attached to the upper surface of the waterproof breathable film (33), a moisture-keeping layer (35) is tightly attached to the upper surface of the water storage layer (34), a groove (37) is arranged at the bottom of the culture tray (3), the groove (37) penetrates through the waterproof breathable film (33), the water storage layer (34) and the moisture retention layer (35) respectively, the outer surface of the groove (37) is tightly attached to the culture cavity (32), a vertical pipe (94) is clamped on the groove (37), and an inclined guide pipe (93) is integrally formed at one end of the vertical pipe (94); a dispersion chamber (92) is integrally formed at one end of the oblique catheter (93), and an introduction funnel (91) is inserted and connected to the upper end of the dispersion chamber (92).

5. The stem cell culture vessel according to claim 1, wherein the inner wall of the culture vessel (2) is provided with a plurality of temperature and humidity sensors (22); an observation window (23) and a controller panel (24) are arranged on the front end surface of the culture container (2); the air circulating pump (6), the stirring motor (52) and the temperature and humidity sensor (22) are respectively electrically connected with the controller panel (24).

6. Stem cell culture vessel according to claim 1, wherein the base (1) is provided with feet (10) at the bottom and the feet (10) are provided with wheels (11) at the bottom.

7. The stem cell culture vessel according to claim 2, wherein the lower liquid tube (57) having a solenoid valve is provided directly above the introduction funnel (91).

8. Stem cell culture vessel according to claim 4, wherein the diameter of the vertical tube (94) is smaller than the diameter of the groove (37).

9. The stem cell culture vessel according to claim 1, wherein the vessel cover (4) is driven by an electric telescopic rod (8) to move up and down, and the electric telescopic rod (8) adopts an NH32-900 electric push rod.

10. The stem cell culture vessel according to claim 4, wherein the waterproof and breathable membrane (3) is a microporous membrane of EPTFE, the water storage layer (4) is a water absorbent cotton cloth, and the moisture-retaining layer (5) is a fiber moisture-retaining layer.

Technical Field

The invention relates to the technical field of culture containers, in particular to a stem cell culture container.

Background

The culture vessel is a laboratory vessel for the cultivation of microorganisms or cells. Stem cells are pluripotent cells having self-replicating ability and can be differentiated into cells with multiple functions under certain conditions, so cultured stem cells are also the subject of current research by microbial researchers, and existing stem cell culture containers are provided with heaters and humidity regulators, but have no micro-air circulation in the culture containers, so that the stem cell culture environment is poor in uniformity during stem cell culture, and power changes such as the heaters and the humidity regulators during the culture process easily interfere with the culture of the stem cells. Secondly, in the process of culturing the stem cells, a culture solution and a differentiation solution need to be added, the liquids need to be heated, the temperature requirements of the culture solution and the differentiation solution are strict, after the culture solution and the differentiation solution are added into a culture dish, the stem cells needing to be cultured can be added after a culture container runs for a period of time, and the operation is inconvenient, so that the culture solution and the differentiation solution are preheated, and the efficiency of culturing the stem cells is necessary to be improved.

Disclosure of Invention

The invention mainly aims to provide a stem cell culture container, wherein a container cover is driven to open by controlling an electric telescopic rod, a culture tray is placed at the inner bottom of the culture container in advance, and a culture lead-in device is placed in the culture tray; during cultivation, firstly, a proper amount of moisture is filled into the cultivation cavity, the cultivation plate is shaken to ensure that the moisture can be fully absorbed by the side surface of the cultivation plate, the moisture is absorbed and stored by the water storage layer through the moisture retention layer, meanwhile, a large amount of moisture is absorbed in the moisture retention layer, the vertical pipe is clamped with the groove, the culture fertilizer is introduced into the culture cavity, then the culture solution and the differentiation solution are added, the liquid is required to be heated, culture solution and differentiation solution are added into a liquid medicine mixing preheating cylinder through a first liquid injection port and a second liquid injection port, electric heaters are respectively started to heat the inside of a water bath heating chamber, a stirring motor is started to start a stirring rod to stir and mix liquid medicine in the liquid medicine mixing preheating cylinder, a liquid descending pipe provided with an electromagnetic valve at the bottom of the liquid medicine mixing preheating cylinder is opened to flow into a guide funnel, and the liquid medicine enters a culture tray through a dispersion chamber, an inclined conduit and a vertical pipe in sequence; finally, the stem cells are poured into the introducing funnel, enter the branch inclined guide pipes through the dispersing cavity and are uniformly distributed in the culture cavity through the vertical pipes, so that the stem cells are convenient to rapidly and appropriately propagate and grow in the environment, and the culture speed is improved; an air exchange pump arranged on the container cover is used for exchanging air inside the culture container, the temperature and humidity sensor which is not arranged in the culture container in the year is electrically connected with a controller panel on the culture container, and the classification control is carried out through the controller panel; the moisture preservation layer provides a proper amount of moisture for the stem cells to grow and reproduce, the gas generated at the bottom is inconvenient to discharge, and the gas can enter the air holes through the waterproof breathable film to be discharged, so that a good environment is provided for the stem cells to grow and reproduce.

In order to achieve the purpose, the stem cell culture container provided by the invention comprises a base, a culture container, a culture tray, a container cover, a liquid medicine mixing preheating cylinder, an air circulating pump and a culture introduction device, wherein the culture container is arranged on the base; the top of the container cover is also provided with an air circulating pump, the air inlet end of the air circulating pump is connected with an air inlet pipe and a first scavenging pipe, and the air outlet end of the air circulating pump is connected with the inside of the culture container through a second scavenging pipe;

the bottom installs the cultivation dish in the culture vessel, is provided with the cultivation gatherer on the cultivation dish, and the middle part still is provided with in the culture vessel and is used for carrying on spacing support to cultivateing the gatherer.

Preferably, the top of the liquid medicine mixing and preheating barrel is provided with a stirring motor, a first liquid injection port and a second liquid injection port, the stirring motor is in driving connection with a stirring rod, one side of the liquid medicine mixing and preheating barrel is provided with an electric heater, and an electric heating sheet electrically connected with the electric heater is arranged in the water bath heating cavity; the bottom of the liquid medicine mixing preheating cylinder is provided with a liquid discharging pipe with an electromagnetic valve.

Preferably, the first ventilating pipe is connected with the air inlet pipe and the culture container; the air inlet pipe and the first scavenging pipe are respectively provided with a first switch valve and a second switch valve.

Preferably, a culture cavity is formed in the culture disc, air holes are formed in the side surface and the bottom surface of the culture disc, waterproof breathable films are bonded on the inner bottom surface and the inner surface of the culture disc, a water storage layer is tightly attached to the upper surface of each waterproof breathable film, a moisture preservation layer is tightly attached to the upper surface of each water storage layer, a groove is formed in the bottom of the culture disc and penetrates through the waterproof breathable films, the water storage layers and the moisture preservation layers respectively, the outer surface of the groove is tightly attached to the culture cavity, a vertical pipe is clamped on the groove, and an inclined guide pipe is integrally formed at one end of the vertical pipe; one end of the inclined conduit is integrally formed with a dispersion cavity, and the upper end of the dispersion cavity is connected with a guide funnel in an embedded manner.

Preferably, a plurality of temperature and humidity sensors are mounted on the inner wall of the culture container; an observation window and a controller panel are arranged on the front end surface of the culture container; air circulating pump, agitator motor and temperature and humidity sensor respectively with controller panel electric connection.

Preferably, the bottom of the base is provided with a support leg, and the bottom of the support leg is provided with a movable wheel.

Preferably, the downcomer with the solenoid valve is disposed directly above the introduction funnel.

Preferably, the diameter of the vertical tube is smaller than the diameter of the groove.

Preferably, the container cover is driven by an electric telescopic rod to move up and down, and the electric telescopic rod adopts an NH32-900 electric push rod.

Preferably, the waterproof breathable film is a microporous material of an EPTFE composite film, the water storage layer is absorbent cotton cloth, and the moisture-keeping layer is a fiber moisture-keeping layer.

According to the technical scheme, the device is convenient to move through the moving wheel, the cover of the container is opened by controlling the driving of the electric telescopic rod, the culture tray is placed at the bottom in the culture container in advance, and the culture lead-in device is placed in the culture tray; during cultivation, firstly, a proper amount of moisture is filled into the cultivation cavity, the cultivation plate is shaken to ensure that the moisture can be fully absorbed by the side surface of the cultivation plate, the moisture is absorbed and stored by the water storage layer through the moisture retention layer, meanwhile, a large amount of moisture is absorbed in the moisture retention layer, the vertical pipe is clamped with the groove, the culture fertilizer is introduced into the culture cavity, then the culture solution and the differentiation solution are added, the liquid is required to be heated, culture solution and differentiation solution are added into a liquid medicine mixing preheating cylinder through a first liquid injection port and a second liquid injection port, electric heaters are respectively started to heat the inside of a water bath heating chamber, a stirring motor is started to start a stirring rod to stir and mix liquid medicine in the liquid medicine mixing preheating cylinder, a liquid descending pipe provided with an electromagnetic valve at the bottom of the liquid medicine mixing preheating cylinder is opened to flow into a guide funnel, and the liquid medicine enters a culture tray through a dispersion chamber, an inclined conduit and a vertical pipe in sequence; finally, the stem cells are poured into the introducing funnel, enter the branch inclined guide pipes through the dispersing cavity and are uniformly distributed in the culture cavity through the vertical pipes, so that the stem cells are convenient to rapidly and appropriately propagate and grow in the environment, and the culture speed is improved; an air exchange pump arranged on the container cover is used for exchanging air inside the culture container, the temperature and humidity sensor which is not arranged in the culture container in the year is electrically connected with a controller panel on the culture container, and the classification control is carried out through the controller panel; the moisture preservation layer provides a proper amount of moisture for the stem cells to grow and reproduce, the gas generated at the bottom is inconvenient to discharge, and the gas can enter the air holes through the waterproof breathable film to be discharged, so that a good environment is provided for the stem cells to grow and reproduce.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.

FIG. 1 is a schematic view of the inside structure of a stem cell culture vessel according to the present invention;

FIG. 2 is a schematic view of a stem cell culture vessel according to the present invention;

FIG. 3 is a schematic view of a culture tray in a stem cell culture vessel according to the present invention;

FIG. 4 is a schematic side sectional view of a culture tray in a stem cell culture vessel according to the present invention;

FIG. 5 is a schematic diagram showing a top view of a culture tray in a stem cell culture vessel according to the present invention;

FIG. 6 is a schematic view showing the structure of the connection of an introduction funnel, an inclined duct and a vertical tube in a stem cell culture vessel according to the present invention.

The reference numbers illustrate:

1. a base; 2. a culture vessel; 3. a culture tray; 4. a container cover; 5. a liquid medicine mixing preheating cylinder; 6. an air circulation pump; 7. a column; 8. an electric telescopic rod; 9. a culture introduction device; 10. a support leg; 11. a moving wheel; 21. a support; 22. a temperature and humidity sensor; 23. an observation window; 24. a controller panel; 32. a culture chamber; 33. a waterproof breathable film; 34. a water storage layer; 35. a moisture retention layer; 36. air holes are formed; 37. a groove; 51. a water bath heating cavity; 52. a stirring motor; 53. a first liquid injection port; 54. a second liquid injection port; 55. a stirring rod; 56. an electric heater; 57. a lower liquid pipe with a solenoid valve; 61. an air inlet pipe; 62. a first ventilation tube; 63. a second ventilation tube; 64. a first on-off valve; 65. a second on-off valve; 91. a lead-in funnel; 92. a dispersion chamber; 93. a deflection conduit; 94. a vertical tube.

The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It should be noted that, if directional indications (such as up, down, left, right, front, and back … …) are involved in the embodiment of the present invention, the directional indications are only used to explain the relative positional relationship between the components, the movement situation, and the like in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indications are changed accordingly.

In addition, if there is a description of "first", "second", etc. in an embodiment of the present invention, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.

As shown in FIGS. 1 to 6, the present invention provides a stem cell culture container, comprising a base 1, a culture container 2, a culture tray 3, a container cover 4, a liquid medicine mixing preheating cylinder 5, an air circulation pump 6 and a culture introducing device 9; a culture container 2 is installed on a base 1, a container cover 4 is hermetically arranged at the top of the culture container 2, stand columns 7 are installed at two ends of the base 1, an electric telescopic rod 8 connected with the culture container 2 is installed on each stand column 7, a liquid medicine mixing preheating cylinder 5 is installed at the top of the container cover 4, a water bath heating cavity 51 is arranged on the inner wall of the liquid medicine mixing preheating cylinder 5, and a stirring rod 55 is arranged inside the liquid medicine mixing preheating cylinder 5; the top of the container cover 4 is also provided with an air circulating pump 6, the air inlet end of the air circulating pump 6 is connected with an air inlet pipe 61 and a first ventilating pipe 62, and the air outlet end of the air circulating pump 6 is connected with the inside of the culture container 2 through a second ventilating pipe 63;

the bottom in the culture container 2 is provided with a culture tray 3, the culture tray 3 is provided with a culture lead-in device 9, and the middle in the culture container 2 is also provided with a bracket 21 for limiting the culture lead-in device 9.

Specifically, the top of the liquid medicine mixing and preheating cylinder 5 is provided with a stirring motor 52, a first liquid injection port 53 and a second liquid injection port 54, the stirring motor 52 is in driving connection with a stirring rod 55, one side of the liquid medicine mixing and preheating cylinder 5 is provided with an electric heater 56, and an electric heating sheet electrically connected with the electric heater 56 is arranged in the water bath heating cavity 51; the bottom of the liquid medicine mixing preheating cylinder 5 is provided with a liquid discharging pipe 57 with an electromagnetic valve.

Specifically, the first ventilation pipe 62 connects the air inlet pipe 61 and the culture container 2; the intake pipe 61 and the first scavenging pipe 62 are provided with a first on-off valve 64 and a second on-off valve 65, respectively.

Specifically, a culture cavity 32 is formed in the culture disc 3, air holes 36 are formed in the side surface and the bottom surface of the culture disc 3, waterproof breathable films 33 are bonded to the inner bottom surface and the inner surface of the culture disc 3, a water storage layer 34 is tightly bonded to the upper surface of the waterproof breathable films 33, a moisture preservation layer 35 is tightly bonded to the upper surface of the water storage layer 34, a groove 37 is formed in the bottom of the culture disc 3, the groove 37 penetrates through the waterproof breathable films 33, the water storage layer 34 and the moisture preservation layer 35 respectively, the outer surface of the groove 37 is tightly bonded to the culture cavity 32, a vertical pipe 94 is clamped on the groove 37, and an inclined guide pipe 93 is integrally formed at one end of the vertical pipe 94; a dispersion chamber 92 is integrally formed at one end of the slant conduit 93, and an introduction funnel 91 is fitted and connected to an upper end of the dispersion chamber 92.

Specifically, a plurality of temperature and humidity sensors 22 are mounted on the inner wall of the culture container 2; an observation window 23 and a controller panel 24 are arranged on the front end face of the culture container 2; the air circulation pump 6, the stirring motor 52 and the temperature and humidity sensor 22 are electrically connected to the controller panel 24, respectively.

Specifically, the bottom of the base 1 is provided with a support leg 10, and the bottom of the support leg 10 is provided with a movable wheel 11.

Specifically, the lower liquid pipe 57 having the solenoid valve is provided directly above the introduction funnel 91.

In particular, the diameter of the vertical tube 94 is smaller than the diameter of the groove 37.

Specifically, the container cover 4 is driven by an electric telescopic rod 8 to move up and down, and the electric telescopic rod 8 adopts an NH32-900 electric push rod.

Specifically, the waterproof breathable film 3 is a microporous material of an EPTFE composite film, the water storage layer 4 is absorbent cotton cloth, and the moisture-retaining layer 5 is a fiber moisture-retaining layer.

The working principle and the using process of the invention are as follows: when the device is used, a worker can conveniently move the device through the moving wheel 11, the cover 4 of the container is driven to be opened by controlling the electric telescopic rod 8, the culture tray 3 is placed at the bottom in the culture container 2 in advance, and the culture lead-in device 9 is placed in the culture tray 3; during cultivation, firstly, a proper amount of moisture is filled into the cultivation cavity 32, the cultivation disc 3 is shaken to enable the moisture to be fully absorbed by the side face of the cultivation disc 3, the moisture is absorbed and stored by the water storage layer 34 through the moisture retention layer 35, meanwhile, a large amount of moisture is also absorbed in the moisture retention layer 35, the vertical pipe 94 is clamped with the groove 37, the cultivation fertilizer is introduced into the cultivation cavity 32, then the cultivation liquid and the differentiation liquid are added, the liquids need to be heated, the cultivation liquid and the differentiation liquid are added into the liquid medicine mixing preheating cylinder 5 through the first liquid injection port 53 and the second liquid injection port 54, the electric heaters 56 are respectively started to heat the inside of the water bath heating cavity 51, the stirring motor 52 is started to start the stirring rod 55 to stir and mix the liquid medicine in the liquid medicine mixing preheating cylinder 5, and then the liquid medicine is opened through the lower liquid pipe 57 provided with an electrified magnetic valve at the bottom of the liquid medicine mixing preheating cylinder 5 and flows into the introducing funnel 91, enters the culture tray 3 through the dispersion cavity 92, the inclined conduit 93 and the vertical pipe 94 in sequence; finally, the stem cells are poured into the introducing funnel 91, enter the branch inclined guide pipes 93 through the dispersing cavity 92 and are uniformly distributed in the culture cavity 32 through the vertical pipes 94, so that the stem cells are convenient to propagate and grow in a fast and proper environment, and the culture speed is improved; an air exchange pump 6 installed on the container cover 4 performs air exchange operation on the inside of the culture container 2, the temperature and humidity sensor 22 which is not installed in the culture container 2 within a year is electrically connected with a controller panel 24 on the culture container 2, and classification control is performed through the controller panel 24; the moisture preservation layer 35 provides a proper amount of moisture for the stem cells to grow and breed, the gas generated at the bottom is inconvenient to discharge, and the gas can enter the air holes 36 through the waterproof breathable film 33 to be discharged, so that a good environment is provided for the stem cells to grow and breed.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.