阅读说明：本技术 一种鉴定作物种子耐淹萌发与幼苗耐淹生长能力的装置 (Device for identifying flooding-resistant germination capacity and flooding-resistant growth capacity of seedlings of crop seeds ) 是由 卢浩宇 李显龙 张玉烛 于 2021-08-23 设计创作，主要内容包括：本发明提出一种鉴定作物种子耐淹萌发与幼苗耐淹生长能力的装置,所述鉴定作物种子耐淹萌发与幼苗耐淹生长能力的装置包括外缸体、内承载框、上压板、开孔PCR板,所述内承载框设置于所述外缸体中,所述开孔PCR板安装于所述内承载框上,所述上压板将所述开孔PCR板压于所述内承载框上。通过本发明可以高通量的模拟不同作物在萌发期至幼苗期对不同淹水胁迫强度下的表型差异,加快科研人员对材料筛选的进度。本装置整体大量使用了标准材料,仅需两种颜色的有机玻璃与标准PCR板即可制作,成本低廉,制备简易。且能切实满足耐淹实验的要求,减少实验误差,提高工作效率,降低工作强度。(The invention provides a device for identifying the flooding-resistant germination capacity of crop seeds and the flooding-resistant growth capacity of seedlings, which comprises an outer cylinder body, an inner bearing frame, an upper pressing plate and an open-pore PCR plate, wherein the inner bearing frame is arranged in the outer cylinder body, the open-pore PCR plate is arranged on the inner bearing frame, and the upper pressing plate presses the open-pore PCR plate on the inner bearing frame. The method can simulate the phenotype difference of different crops to different flooding stress strengths from the germination stage to the seedling stage with high flux, and accelerate the speed of scientific research personnel in screening materials. The device integrally uses a large amount of standard materials, can be manufactured only by organic glass with two colors and a standard PCR plate, and has low cost and simple and easy preparation. And the requirements of the flooding-resistant experiment can be practically met, the experiment error is reduced, the working efficiency is improved, and the working strength is reduced.)

1. The device for identifying the flooding-resistant germination capacity of crop seeds and the flooding-resistant growth capacity of seedlings is characterized by comprising an outer cylinder body, an inner bearing frame, an upper pressing plate and an opening PCR plate, wherein the inner bearing frame is arranged in the outer cylinder body, the opening PCR plate is arranged on the inner bearing frame, and the upper pressing plate presses the opening PCR plate on the inner bearing frame.

2. The device for identifying the flood-resistant germination of crop seeds and the flood-resistant growth capacity of seedlings according to claim 1, wherein the external cylinder body comprises two transverse cylinder walls, two longitudinal cylinder walls and a cylinder bottom, the number of the transverse cylinder walls and the number of the longitudinal cylinder walls are two, the two transverse cylinder walls are arranged in parallel, the two longitudinal cylinder walls are arranged in parallel, the transverse cylinder walls and the longitudinal cylinder walls are arranged vertically, the two transverse cylinder walls and the two longitudinal cylinder walls are connected end to end and vertically surround the cylinder bottom, the transverse cylinder walls and the longitudinal cylinder walls are made of transparent organic glass, and the cylinder bottom is made of black opaque organic glass.

3. The device for identifying the flood-resistant germination of crop seeds and the flood-resistant growth capability of seedlings as claimed in claim 2, wherein the number of the inner bearing frames is two, two inner bearing frames are disposed on the cylinder bottom, one sides of the two inner bearing frames far away from each other are respectively abutted against the two longitudinal cylinder walls, one sides of the two inner bearing frames near to each other are disposed at intervals and are respectively provided with a limiting clamping block, and the inner bearing frames are made of black opaque organic glass.

4. The device for identifying the flood germination resistance and the flood growth resistance of seedlings of crop seeds as claimed in claim 3, wherein the upper pressing plate comprises a separation part and a pressing part, the separation part comprises two separation plates parallel to the longitudinal cylinder wall, the two separation plates are arranged between the two inner bearing frames, one side of each of the two separation plates close to the inner bearing frame is provided with a limiting clamping hole, the limiting clamping holes are arranged corresponding to the limiting clamping blocks, the pressing part comprises two pressing plates parallel to the transverse cylinder wall, the two pressing plates are respectively arranged at two ends of the separation part, the pressing plates are of a structure with a wide upper part and a narrow lower part, the pressing plates comprise an upper plate and a lower plate, the lower plate is connected to the middle part of the upper plate, and the width of the lower plate is equal to the spreading width of the two separation plates, the upper plate butt is in two the edge of interior bearing frame, the top board adopts transparent organic glass material.

5. The apparatus of claim 4, wherein the apertured PCR plates cooperate with the bottom of the cylinder to form light-blocking root system development sites, and each apertured PCR plate is provided with 96 monitoring holes for identification.

6. The device for identifying the flooded germination and flooded growth resistance of crop seeds as claimed in any one of claims 1 to 5, wherein the outer layer of the cylinder wall is marked with graduations.

7. The device for identifying the flood germination resistance and the flood growth resistance of seedlings of crop seeds as claimed in any one of claims 1 to 5, wherein a liquid injection groove is reserved in the middle of the upper pressing plate.

Technical Field

The invention belongs to the technical field of plant culture, and particularly relates to a device for identifying flood-resistant germination of crop seeds and flood-resistant growth of seedlings.

Background

The flood-resistant improvement of crops is always an important direction for the improvement of crop varieties, the improvement of the flood-resistant characteristic of the germination period and the seedling growth period of crop seeds can improve the survival rate of crops under the stress of flooding, and the planting benefit of farmers is guaranteed. Therefore, the identification of the flooding resistance of the crop seeds in the germination stage and the seedling stage is the key work for improving the variety. And how to accurately, efficiently and conveniently identify the flooding-resistant characteristic is particularly important.

At present, scientific researchers identify flooding resistance of crop seeds and seedlings in two aspects, namely field identification and indoor identification.

The field identification means that the identification material is placed in the natural environment of the field for flooding stress, and the related result is obtained. The general operation is: and sowing the germinated seeds in a field, and performing flooding stress of a corresponding depth after sowing or after seedling growth according to the requirement of flooding identification. However, the field identification is affected by factors such as weather environment, the identification result often has obvious errors, and the accurate and reliable identification result is obtained.

Most scientific researchers in indoor identification use a test tube or a centrifugal tube as a carrier for identifying the crop flooding capacity. The general operating method is as follows: the seeds are put into a test tube or a centrifugal tube after pregermination, distilled water for identifying the corresponding depth is added, finally, the seeds are put into a set artificial climate box for culture, water is changed in the midway, the relative phenotype and physiological index of the rice seedlings are measured after the time required by identification is reached, the difference of the flooding resistance between different rice is reflected by the indexes, and the required rice material is screened out. However, there are many disadvantages to the identification by test tubes or centrifuge tubes:

firstly, the amount of seeds placed is large and small, the result of the identification of the flooding resistance is influenced, excessive seeds are placed, the insides of the seeds compete with each other, meanwhile, the seeds are extruded after growing out of roots due to the excessive seeds, the seeds climb upwards, the flooding depth in the identification standard has obvious errors, and the identification result is not credible; secondly, if the seeding amount in a single test tube is reduced, the using amount of the test tube is greatly increased, and the workload of the identification process is increased; and thirdly, no matter how many seeds are, with the germination of rice seeds, the root system grows downwards and is damaged due to natural bottom sinking of the seeds, so that the root system grows to be in an unnatural state and the root system lifts up the seeds by 1-3 cm. This also leads to errors in the identification criteria; and fourthly, when the test tube and the centrifuge tube are used, the seeds can be impacted by overlarge water flow when water is changed, so that the root system or the seedling of the seeds are damaged and can move.

In order to solve the problems of low accuracy, large workload and low efficiency of the existing flood-resistant capability identification of crop seeds, a device for identifying the flood-resistant germination capability and the flood-resistant growth capability of seedlings of the crop seeds is needed.

Disclosure of Invention

In view of the problems in the background art, the invention aims to provide a device for identifying the flooding-resistant germination capacity and the flooding-resistant growth capacity of seedlings of crop seeds, and aims to solve the problems of low accuracy, large workload and low efficiency of the existing flooding-resistant capacity identification of the crop seeds.

In order to achieve the purpose, the invention adopts the following technical scheme:

a device for identifying the flooding-resistant germination capacity of crop seeds and the flooding-resistant growth capacity of seedlings comprises an outer cylinder body, an inner bearing frame, an upper pressing plate and an open-pore PCR plate, wherein the inner bearing frame is arranged in the outer cylinder body, the open-pore PCR plate is arranged on the inner bearing frame, and the upper pressing plate presses the open-pore PCR plate on the inner bearing frame.

Preferably, the external cylinder body includes horizontal cylinder wall, indulges cylinder wall and cylinder bottom, horizontal cylinder wall with indulge the quantity of cylinder wall and be two, two horizontal cylinder wall parallel arrangement, two indulge cylinder wall parallel arrangement, horizontal cylinder wall with indulge the cylinder wall and set up perpendicularly, two horizontal cylinder wall and two indulge the cylinder wall end to end connection and encircle perpendicularly the cylinder bottom sets up, horizontal cylinder wall with indulge the cylinder wall and all adopt transparent organic glass material, the cylinder bottom adopts the opaque material of black organic glass.

Preferably, the quantity of interior bearing frame is two, two interior equal bearing frame set up in on the cylinder bottom, two one side that interior bearing frame kept away from each other is two the butt respectively indulges the cylinder wall, two one side interval that interior bearing frame is close to each other sets up and all installs spacing joint piece, interior bearing frame adopts black opaque organic glass material.

Preferably, the top board includes isolation part and compresses tightly the portion, the isolation part includes two and is on a parallel with indulge the division board that the jar wall set up, two the division board sets up in two between the interior bearer frame, two the division board is close to spacing joint hole has all been seted up to one side of interior bearer frame, spacing joint hole with spacing joint piece corresponds the setting, the portion that compresses tightly includes two and is on a parallel with the pressure strip that violently jar wall set up, two the pressure strip set up respectively in the isolation part both ends, just the pressure strip is narrow type structure under the wide, the pressure strip includes upper plate and hypoplastron, the hypoplastron connect in the upper plate middle part, hypoplastron width and two the division board struts the width parallel and level, the upper plate butt is two the edge of interior bearer frame, the top board adopts transparent organic glass material.

Preferably, the open-cell PCR plate and the cylinder bottom are matched to form a root system development position for isolating light, and 96 monitoring hole positions are respectively provided on each open-cell PCR plate for identification.

Preferably, the outer layer of the cylinder wall is marked with a scale.

Preferably, a liquid injection groove is reserved in the middle of the upper pressure plate.

The beneficial effects of the invention mainly comprise: the method can simulate the phenotype difference of different crops to different flooding stress strengths from the germination stage to the seedling stage with high flux, and accelerate the speed of scientific research personnel in screening materials. The device integrally uses a large amount of standard materials, can be manufactured only by organic glass with two colors and a standard PCR plate, and has low cost and simple and easy preparation. And the requirements of the flooding-resistant experiment can be practically met, the experiment error is reduced, the working efficiency is improved, and the working strength is reduced.

Drawings

FIG. 1 is a schematic diagram of the overall structure of an apparatus for identifying the flood germination resistance and the flood growth resistance of seedlings of crop seeds according to an embodiment of the present invention;

FIG. 2 is an expanded schematic view of the apparatus for identifying flood germination and flood growth tolerance of crop seeds after the upper platen is removed according to one embodiment of the present invention;

fig. 3 is a schematic structural view of the upper platen of the present invention with one side of the hold-down plate omitted.

The reference numbers illustrate:

reference numerals	Name (R)	Reference numerals	Name (R)
				10	Outer cylinder body	311	Partition board
11	Transverse cylinder wall	312	Spacing joint hole
				12	Longitudinal cylinder wall	32	Pressing part
13	Cylinder bottom	321	Pressing tightlyBoard
				20	Inner bearing frame	3211	Upper plate
21	Spacing joint block	3212	Lower plate
				30	Upper pressure plate	40	Perforated PCR plate
31	Isolation part	41	Monitoring hole sites

Detailed Description

The present invention will be further described with reference to the accompanying drawings so as to facilitate the understanding of the present invention by those skilled in the art.

It should be noted that all the directional indicators (such as up, down, left, right, front, and rear … …) in the embodiment of the present invention are only used to explain the relative position relationship between the components, the movement situation, etc. in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indicator is changed accordingly.

In addition, the descriptions related to "first", "second", etc. in the present invention are only for descriptive purposes and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

The description of the orientations of "up", "down", "front", "back", "left", "right", etc. in the present invention is based on the orientations shown in fig. 1 to 3, and is only used to explain the relative positional relationship between the respective components in the postures shown in fig. 1 to 3, and if the specific posture is changed, the directional indication is correspondingly changed accordingly.

The invention provides a device for identifying flood germination resistance and flood growth resistance of seedlings of crop seeds, which comprises an outer cylinder body 10, an inner bearing frame 20, an upper pressure plate 30 and a perforated PCR plate 40, wherein the inner bearing frame 20 is arranged in the outer cylinder body 10, the perforated PCR plate 40 is arranged on the inner bearing frame 20, and the perforated PCR plate 40 is pressed on the inner bearing frame 20 by the upper pressure plate 30.

Specifically, the method can simulate the phenotype difference of different crops under different flooding stress strengths from the germination stage to the seedling stage in a high-throughput manner, and accelerate the progress of scientific research personnel in material screening. The device integrally uses a large amount of standard materials, can be manufactured only by organic glass with two colors and a standard PCR plate, and has low cost and simple and easy preparation. And the requirements of the flooding-resistant experiment can be practically met, the experiment error is reduced, the working efficiency is improved, and the working strength is reduced.

Further, the external cylinder body 10 comprises two transverse cylinder walls 11, two longitudinal cylinder walls 12 and a cylinder bottom 13, the number of the transverse cylinder walls 11 and the number of the longitudinal cylinder walls 12 are two, the two transverse cylinder walls 11 are arranged in parallel, the two longitudinal cylinder walls 12 are arranged in parallel, the transverse cylinder walls 11 and the longitudinal cylinder walls 12 are arranged vertically, the two transverse cylinder walls 11 and the two longitudinal cylinder walls 12 are connected end to end and vertically surround the cylinder bottom 13, the transverse cylinder walls 11 and the longitudinal cylinder walls 12 are made of transparent organic glass, and the cylinder bottom 13 is made of black opaque organic glass. Interior bearing frame 20's quantity is two, and equal bearing frame sets up on cylinder bottom 13 in two, and two longitudinal cylinder walls 12 of one side butt respectively that two interior bearing frames 20 kept away from each other, and spacing joint piece 21 is just all installed to one side interval that two interior bearing frames 20 are close to each other, and interior bearing frame 20 adopts black opaque organic glass material. The top board 30 includes isolation portion 31 and clamping portion 32, isolation portion 31 includes two division boards 311 that are on a parallel with indulge cylinder wall 12 and set up, two division boards 311 set up in two between the bearer frame 20, spacing joint hole 312 has all been seted up to one side that two division boards 311 are close to interior bearer frame 20, spacing joint hole 312 corresponds the setting with spacing joint piece 21, clamping portion 32 includes two pressure strips 321 that are on a parallel with horizontal cylinder wall 11 and set up, two pressure strips 321 set up respectively in isolation portion 31 both ends, and pressure strip 321 is narrow type structure under the upper width, pressure strip 321 includes upper plate 3211 and hypoplastron 3212, hypoplastron 3212 connects in upper plate 3211 middle part, hypoplastron 3212 width and two division boards 311 strut the width parallel and level, the edge of bearer frame 20 is born in two to upper plate 3211 butt, top board 30 adopts transparent organic glass material. The open-cell PCR plates 40 are matched with the cylinder bottom 13 to form root system development positions for isolating light, and 96 monitoring hole sites 41 are respectively provided on each open-cell PCR plate 40 for identification. The outer layer of the cylinder wall is marked with scales. A liquid injection groove is reserved in the middle of the upper pressure plate 30.

Specifically, as shown in fig. 1 to 3, the external cylinder body 10 is made of a transparent organic glass cylinder wall and a black opaque cylinder bottom 13, and is durable and has good light transmittance. The outer layer is marked with scales, so that the growth condition and the water level condition of the sample can be directly observed, and the illumination condition of the sample is not influenced. The inner carrier frame 20 is made of black opaque plexiglass, completely protected from light. Mainly play the effect of bearing trompil PCR board 40, and guarantee the isolated light of cylinder body the latter half with the cooperation of cylinder bottom 13, protect the root system to avoid illumination to influence. The upper press plate 30 is made of transparent organic glass, and can fix the PCR plate by its own weight after being loaded into the outer cylinder body 10, thereby avoiding the problem that the PCR plate floats after water injection. The middle part is provided with a liquid injection groove, so that the liquid supplementing operation is convenient, and the influence of the liquid supplementing on the plant growth is avoided. Trompil PCR board 40 is through trompil standard PCR board lower extreme, comes as the seed bearing body of high flux, can be through embracing the fixed seed position of seed, guarantees that the sample position is unified, also can guide root system natural growth through the lower extreme trompil, stops that the root system that the seed nature sinks the end to lead to grows downwards impaired, simultaneously because the equal independent bearing of seed in every hole, can not extrude the influence mutually between the seed, very big reduction the interference between the sample, guaranteed the reliability of sample. And the opening size of the PCR plate can be adjusted at will according to the size of the sample so as to deal with the difference between different seeds, thereby improving the universality.

The specific using method comprises the following steps:

1) different crop seeds are respectively placed in monitoring hole sites 41 in the perforated PCR plate 40 from the germination stage to the seedling stage;

2) carrying out flooding stress intensity of different degrees on the monitoring hole position 41 respectively;

3) and respectively recording the performance difference of different crop seeds from the germination stage to the seedling stage in response to different flooding stress strengths.

The above description is only a preferred embodiment of the present invention, and for those skilled in the art, the present invention should not be limited by the description herein, since various changes and modifications can be made in the details of the embodiment and the application range according to the spirit of the present invention.