Rapid budding method for cutting asparagus seedlings
阅读说明：本技术 一种龙须菜苗种切段的快速出芽方法 (Rapid budding method for cutting asparagus seedlings ) 是由 钟晨辉 林琪 佘婷婷 唐隆晨 周文发 于 2021-09-24 设计创作，主要内容包括：本发明公开了一种龙须菜苗种切段的快速出芽方法,包括如下步骤：1)选择完整、健康的龙须菜,清洗藻体,去除附生杂藻；选取藻体主杆用消毒刀片剔除侧枝,切成长度为1.2-1.8cm的藻段；2)配置盐度为0-6.5％的低盐培养液；3)藻段在低盐培养液中浸泡1-6h；4)藻段在正常盐度培养液中恢复培养。采用本发明的方法,不仅出芽快,出芽数量增加,且相对生长速率增加。(The invention discloses a quick sprouting method for cutting asparagus seedlings, which comprises the following steps: 1) selecting complete and healthy asparagus, cleaning the frond and removing epiphytic mixed fronds; selecting main stems of algae, removing side branches with a disinfection blade, and cutting into sections with the length of 1.2-1.8 cm; 2) preparing a low-salt culture solution with the salinity of 0-6.5%; 3) soaking the algae segments in low-salt culture solution for 1-6 h; 4) the algae section is restored to be cultured in the culture solution with normal salinity. By adopting the method, the germination is fast, the germination quantity is increased, and the relative growth rate is increased.)
1. A quick sprouting method for cutting asparagus seedlings comprises the following steps:
1) selecting complete and healthy asparagus, cleaning the frond and removing epiphytic mixed fronds; selecting main stems of algae, removing side branches with a disinfection blade, and cutting into sections with the length of 1.2-1.8 cm;
2) preparing a low-salt culture solution with the salinity of 0-6.5%;
3) soaking the algae segments in low-salt culture solution for 1-6 h;
4) the algae section is restored to be cultured in the culture solution with normal salinity.
2. The method for rapid sprouting of cut pieces of asparagus seedlings according to claim 1, characterized in that: soaking the algae segments in low-salt culture solution for 3-6 h.
3. The method for rapid sprouting of cut pieces of asparagus seedlings according to claim 1, characterized in that: soaking the algae segments in low-salt culture solution for 3 h.
4. The method for rapid sprouting of cut pieces of asparagus seedlings according to any one of claims 1 to 3, characterized in that: in the step 1), the complete and healthy asparagus is firstly heated to 22 +/-0.5 ℃ and the illumination intensity is 15 mu mol.m-2·s-1(ii) a Culturing in Provasoli medium with illumination period of 12L: 12D and salinity of 26 ‰, for 1-3 weeks, and replacing every 3D.
5. The method for rapid sprouting of cut pieces of asparagus seedlings according to claim 1, characterized in that: the low-salt culture solution is prepared by adding water into a Provasoli culture medium for dilution.
Technical Field
The invention relates to a quick sprouting method for cutting a asparagus seedling into sections.
Background
Salinity is one of the important ecological factors for regulating the growth and development of the seaweed and determines the distribution condition of the seaweed. The effects of salinity stress on seaweed have been extensively reported. In 1999, Martins et al found that salinity is related to the growth rate of Enteromorpha intestinalis (Enteromorpha) at the estuary of Mondego (Portugal), the growth rate of Enteromorpha intestinalis is low when the salinity of seawater is lower than 5psu or higher than 25psu, and algae die when the salinity is reduced to below 1 psu; helihong et al (2002) found that, through the research on Gracilaria tenuistipita var. liui, too high or too low salinity will affect the yield and quality of Gracilaria agar; related studies have also shown that an increase in salinity over a certain range can cause accumulation of mannitol in laver (porphyrra); PS II maximum photochemical efficiency (Fv/Fm) of Sargassum thunbergii (Sargassum thunbergii) is significantly reduced under high salt stress, and rapidly returns to normal level after transferring to normal seawater for culture.
Gracilaria lemaneiformis (Gracilariopsis lemaneiformis) is one of important seaweed cultivation varieties in China and has important economic and ecological values. At present, the cultivation of asparagus is mainly carried out by means of vegetative propagation. Although vegetative propagation is fast in growth and simple in operation, the high-temperature period in summer in south China is long, the growth period of the frond is short, and the industrial development still faces the problem that the production of the offspring seeds needs to be accelerated. How to improve the growth rate of the asparagus by optimizing a seedling breeding mode in limited suitable cultivation time has great industrial practice significance for increasing the yield of the asparagus.
Disclosure of Invention
The invention mainly aims to provide a quick sprouting method for cutting asparagus seedlings.
The technical scheme adopted by the invention for solving the technical problem is as follows:
a quick sprouting method for cutting asparagus seedlings comprises the following steps:
1) selecting complete and healthy asparagus, cleaning the frond and removing epiphytic mixed fronds; selecting main stems of algae, removing side branches with a disinfection blade, and cutting into sections with the length of 1.2-1.8 cm;
2) preparing a low-salt culture solution with the salinity of 0-6.5%, wherein when the salinity is 0, the low-salt culture solution is water;
3) soaking the algae segments in low-salt culture solution for 1-6 h;
4) the algae section is restored to be cultured in the culture solution with normal salinity.
Preferably, the algal pieces are soaked in a low-salt culture solution for 3-6 hours.
Further preferably, the algal pieces are soaked in a low-salt culture solution for 3 hours.
Preferably, in step 1), the whole and healthy asparagus is firstly heated at 22 +/-0.5 ℃ and the illumination intensity is 15 mu mol.m-2·s-1(ii) a Culturing in Provasoli medium with illumination period of 12L: 12D and salinity of 26 ‰, for 1-3 weeks, and replacing every 3D.
Preferably, the low-salt culture solution is prepared by diluting the Provasoli culture medium with water.
Compared with the background technology, the technical scheme of the invention has the following advantages:
by adopting the method, the germination is fast, the germination quantity is increased, and the relative growth rate is increased.
Drawings
The invention is further illustrated by the following figures and examples.
FIG. 1 shows the germination numbers of Gracilaria verrucosa sections recovered to normal salinity culture solution after different salinity stress for different time periods and cultured for 28 days in examples and comparative examples.
FIG. 2 shows the effect of low salt treatment on growth of Gracilaria verrucosa in different examples and comparative examples (28 d).
FIGS. a-u are respectively: a. a control group; b. fresh water treatment is carried out for 1 h; c. fresh water treatment is carried out for 3 h; d. fresh water treatment is carried out for 6 h; e. fresh water treatment is carried out for 12 h; f. treating saline water with salinity of 6.5 per mill for 1 hour; g. treating saline water with salinity of 6.5 per mill for 3 hours; h. treating saline water with salinity of 6.5 per mill for 6 hours; i. treating saline water with salinity of 6.5 per mill for 12 hours; j. treating saline water with salinity of 6.5 per mill for 20 hours; k. treating saline water with salinity of 13 per mill for 1 hour; saline water with salinity of 13 per mill is treated for 3 hours; treating saline water with salinity of 13 per mill for 6 hours; n, treating saline water with salinity of 13 per mill for 12 hours; treating saline water with salinity of 13 per mill for 20 hours; treating saline water with salinity of 19.5 per mill for 1 hour; treating saline water with salinity of 19.5 per mill for 3 hours; treating saline water with salinity of 19.5 per mill for 6 hours; s, treating saline water with salinity of 19.5 per mill for 12 hours; t, treating saline water with the salinity of 19.5 per mill for 20 hours; u. asparagus is stressed for 20h in fresh water, and all the fronds die in 4 days.
FIG. 3 Effect of fresh water stress on Fv/Fm in Gracilaria lemaneiformis cutting regeneration process
Note: before algal section treatment (OS), after Stress (ST), 2d (R2) of normal salinity restoring culture, 6d (R6) of normal salinity restoring culture, 11d (R11) of normal salinity restoring culture, 16d (R16) of normal salinity restoring culture and 21d (R21) of normal salinity restoring culture.
Detailed Description
Raw materials: the asparagus is a new variety of Lulong No. 1, is collected from the Nanrijima sea area of Pu Tian city in Fujian province, and is transported back to the laboratory under the shady and cool conditions in the dark. Cleaning the surface of the asparagus by natural sterilized seawater, scrubbing and removing the attached foreign algae on the surface by a soft hair brush, and culturing the asparagus in an indoor laboratory for 2 weeks by the sterilized seawater after the cleaning is finished so as to adapt to the laboratory environment. The culture conditions are as follows: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 15 mu mol.m-2·s-1(ii) a The illumination period is 12L: 12D, the salinity is 26 per mill, the culture solution is Provasoli culture medium, and the culture solution is replaced every 3D.
Material treatment: selecting complete and healthy asparagus, cleaning the frond and removing epiphytic mixed fronds. Selecting main stems of algae (lateral branches are removed by a disinfection blade), and cutting the main stems into algae segments with the length of 1.5cm for experiment.
Preparing a low-salt culture solution: distilled water is 0 per mill; adding distilled water into Provasoli culture medium (Control, 26 ‰) commonly used in laboratory, and diluting culture solution with salinity of 19.5 ‰, 13 ‰, and 6.5 ‰ at 3:1, 1:1, and 1:3 respectively.
Example 1 salinity 0%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 0 per mill for 1h, and immediately returning to the normal salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Example 2 salinity 0%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 0 per mill for 3h, and immediately returning to the normal salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Example 3 salinity 0%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 0 per mill for 6h, and immediately returning to the normal salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 1 salinity of 0%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment by 0 per mill for 12h, and immediately returning to the normal salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 2 salinity of 0%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment by 0 per mill for 20h, and immediately returning to the normal salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Unified control culture during experimentThe culture conditions are as follows: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture solution is Provasoli culture medium (salinity of 26 ‰), and the culture solution is replaced every 3D.
Example 4 salinity 6.5%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 6.5 per mill for 1h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Example 5 salinity 6.5%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 6.5 per mill for 3h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Example 6 salinity 6.5%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 6.5 per mill, setting the treatment time to be 6h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 3 salinity of 6.5%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 6.5 per mill for 12h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 4 salinity 6.5%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 6.5 per mill for 20h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 5 salinity 13.0%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 13.0 per mill for 1h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 6 salinity 13.0%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 13.0 per mill for 3h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Unified control during experimentPreparing culture conditions: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 7 salinity 13.0%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 13.0 per mill for 6h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 8 salinity 13.0%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 13.0 per mill for 12h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 9 salinity 13.0%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 13.0 per mill for 20h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 10 salinity 19.5%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 19.0 per mill, setting the treatment time to be 1h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 11 salinity 19.5%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 19.0 per mill for 3h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 12 salinity 19.5%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 19.0 per mill for 6h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 13 salinity 19.5%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 19.0 per mill for 12h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Fruit of Chinese wolfberryThe culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 14 salinity 19.5%
And (3) transferring 20 algae segments/group which are cut in advance into a 180mL transparent plastic cup containing 150mL of culture solution, carrying out low-salt stress treatment for 19.0 per mill, setting the treatment time to be 20h, and immediately returning to the normal-salinity culture solution for culturing for 28d after the stress is finished. Each set of treatments set 3 biological replicates. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the normal salinity culture medium is Provasoli culture medium (salinity of 26 ‰), and the culture medium is replaced every 3D.
Comparative example 15 salinity 26%
Stress design: 20 algae segments/group which are cut in advance are respectively transferred into a transparent plastic cup with the specification of 180mL containing 150mL of culture solution and cultured in 26.0 thousandth for 25d, and 3 biological repetitions are set for each group of treatment. Culture conditions were controlled uniformly during the experiment: the temperature is 22 +/-0.5 ℃, and the illumination intensity is 30 mu mol.m-2·s-1The illumination period is 12L: 12D, the culture medium is Provasoli culture medium, and the culture medium is replaced every 3D.
All examples and comparative examples were tested every 3 days for growth indicators (including fresh mass FW and total number of shoots TB) and the growth was recorded by camera for an experimental period of 28 days.
The change in biomass (fresh weight, FW) of the asparagus algae pieces over the experimental period was calculated to assess their growth. The Relative Growth Rate (RGR) is calculated as follows:
in the formula, WO: fresh weight (g) of algal pieces at the start of the experiment; wt: fresh weight (g) of algal pieces at the end of the experiment; t: days of experiment.
The results show that:
observing the growth of all comparative and example Gracilaria verrucosa sections within the experimental period (28d), the examples are short-term (t)<12h) The low salt stress can accelerate the sprouting speed of the asparagus and increase the sprouting number of the cut segments of the alga body, and the total sprouting number is higher than that of the comparative example. The stress of fresh water (0 per thousand, examples 1-3) for 3h to 6h has the best effect, and the number of buds is the most; the fresh water stress exceeds 20h (comparative example 2), the fronds become whitish and fragmented from the third day, and the fronds die out (figure 1, figure 2). The total number of buds influences the change of the fresh quality (FW) of the asparagus algae, the faster the buds are germinated, the more the buds are germinated, and the faster the FW is increased in the subsequent culture, the more obvious the growth advantage of the algae is. The asparagus algae segments are recovered to be cultured in a culture solution with normal salinity for 28 days after being tolerant to low salt stress for different time under different salinity, and the increasing conditions of algae segment FW are obviously different (Table 1). Algal sections with freshwater stress time less than 6h gained more (examples 1-3), corresponding to high Relative Growth Rate (RGR), significantly different (P) from the control (comparative example 15)<0.05). When the strain is cultured for 28 days, the FW of the algae section is increased greatly after the strain is stressed for 3 hours (example 3), the growth speed is fastest, and the GRG reaches 0.9149%. d-1The improvement is 61.2727% compared with the control group; the alga FW with the fresh water stress time more than or equal to 12h is increased negatively, and the alga color gradually fades and festers due to the fact that the alga absorbs a large amount of water and lasts for too long time, and finally dies. The algae segment stressed most seriously after 20h of fresh water stress, and the relative growth rate is-8.6191%. d-1The algae segment died completely in a short time (part of albino algae segment remained). Comparative example 4 has slightly high salinity, longer treatment time and better sprouting effect, but the treatment time is long, and if a large amount of the salt is accumulated together in production and the soaking time is longer, the salt is easy to rot off, and the risk is greatly increased.
The experimental result shows that the short-time low-salt stress has the effect of promoting the regeneration of the cut segments of the Gracilaria lemaneiformis, and the effect is best when the fresh water is stressed for 3 hours.
TABLE 1 fresh mass (FW) increase after low salt stress of Gracilaria verrucosa sections and recovery to normal salinity culture medium for 28 days
TABLE 2 relative growth rate of Gracilaria verrucosa segments after fresh water stress and recovery to normal salinity culture fluid within 28 days
The trend of Fv/Fm change in the process of restoring Gracilaria verrucosa sections to normal salinity culture solution after fresh water stress at different times is shown in FIG. 3. The short-time (1-3 h) fresh water stress causes the Fv/Fm of the algae section to slightly decrease but still approach the level of the control group, and the algae section can recover to the level of the control group even slightly higher than the control group after being transferred to a normal salinity culture solution for culture, which indicates that the asparagus can be well adapted to the short-time low-salt stress and the short-time low-salt stress has no significant negative effect on the photosynthetic activity of the asparagus. When the freshwater is stressed for 20 hours, the Fv/Fm value of the alga section is rapidly reduced to be close to 0, and the alga section is transferred into a normal salinity culture solution to be cultured and cannot be recovered, which indicates that the asparagus alga section is irreversibly damaged under the long-time freshwater stress.
The above description is only a preferred embodiment of the present invention, and therefore should not be taken as limiting the scope of the invention, which is defined by the appended claims and their equivalents.