阅读说明：本技术 一种猪精液的采集、稀释及分装方法 (Method for collecting, diluting and subpackaging boar semen ) 是由 张亮 陈力 吴平先 范志连 张利娟 柴捷 李兴桂 吴玲 肖杰秋 徐荣 刘书伦 于 2021-09-27 设计创作，主要内容包括：本申请涉及猪的采精设备技术领域,公开了一种猪精液的采集、稀释及分装方法,其特征在于,具体包括以下步骤：步骤1,采精后称重；步骤2,将精液在稀释袋内充分混匀；步骤3,通过取样口吸取原精进行检查；步骤4,经检查合格后,通过软管注入稀释液并摇匀稀释；步骤5,通过所述精液收集套盒上的软管将精液分装到精液分装袋中。具有精液在采精、稀释及分装过程中不易被污染的优点。(The application relates to the technical field of pig semen collection equipment, and discloses a pig semen collection, dilution and subpackage method, which is characterized by comprising the following steps: step 1, weighing after semen collection; step 2, fully and uniformly mixing the semen in the dilution bag; step 3, sucking the original essence through a sampling port for inspection; step 4, after the inspection is qualified, injecting diluent through a hose and shaking up for dilution; and 5, subpackaging the semen into semen subpackage bags through the hoses on the semen collection sleeve boxes. Has the advantage that the semen is not easy to be polluted in the processes of semen collection, dilution and subpackage.)

1. A method for collecting, diluting and subpackaging boar semen is characterized by comprising the following steps:

step 1, inserting penis of a pig into the semen collector (1), collecting semen of the pig by using the semen collector (1), taking down the semen collection sleeve box (3) after the pig fully ejaculates, and weighing the weight of the semen collection sleeve box (3);

step 2, fully and uniformly mixing the semen in a dilution bag (6);

step 3, sucking 0.5-1 ml of original semen through a sampling port (8) on the semen collection sleeve box (3) for inspection;

step 4, after the test is qualified, connecting the hose (2) with a connecting port (7) on a dilution bag (6), injecting a diluent through the hose (2), and shaking up for dilution;

and 5, subpackaging 40ml-100ml of semen into the semen subpackaging bag through the hose (2) on the semen collecting sleeve box (3).

2. The method for collecting, diluting and dispensing boar semen according to claim 1, wherein: in the step 2, the semen collection sleeve box (3) is placed on a mechanical arm, and the mechanical arm rotates at a constant speed at an angular speed of 90 degrees/s-360 degrees/s, so that the semen is fully mixed in the diluent bag (6).

3. The method for collecting, diluting and dispensing porcine semen according to claim 1 or 2, characterized in that: in step 4, a peristaltic pump is used to slowly inject diluent into the dilution bag (6).

4. The method for collecting, diluting and dispensing boar semen according to claim 3, wherein: in the step 5, 40ml to 100ml of semen is slowly subpackaged into semen subpackaging bags by using a peristaltic pump.

Technical Field

The invention relates to the technical field of pig semen collection equipment, in particular to a method for collecting, diluting and subpackaging pig semen.

Background

With the development and progress of animal husbandry, the breeding industry adopts a plurality of advanced methods to improve efficiency to create more benefits, the reproductive capacity of boars is continuously enhanced along with the progress of artificial semen collection, in order to provide better-quality pork for people, the semen collection efficiency of boars is improved by the artificial semen collection method, but the semen of boars is easily polluted by bacteria in the semen collection, dilution and subpackaging processes, so that the sperm activity is low, and even the sperm is dead in a large amount and cannot be fertilized.

Disclosure of Invention

The invention aims to provide a method for collecting, diluting and subpackaging boar semen, which aims to solve the problem that the semen is polluted by bacteria in the processes of semen collection, dilution and subpackaging.

In order to achieve the aim, the invention provides a method for collecting, diluting and subpackaging boar semen, which is characterized by comprising the following steps:

step 1, inserting penis of a pig into a semen collector, collecting semen of the pig by using the semen collector, taking down the semen collection sleeve box after the pig completely ejaculates, and weighing the weight of the semen collection sleeve box;

step 2, fully and uniformly mixing the semen in the dilution bag;

step 3, sucking 0.5-1 ml of original semen through a sampling port on the semen collection sleeve box for inspection;

step 4, after the test is qualified, connecting a hose with a connecting port on the dilution bag, injecting a diluent through the hose, diluting and shaking up;

and 5, subpackaging 40ml-100ml of semen into the semen bag through a hose on the semen collection sleeve box.

In order to mix the semen more evenly, in step 2, the semen collection sleeve is placed on a mechanical arm which rotates at a constant speed at an angular speed of 90 °/s-360 °/s, so that the semen is mixed in the dilution bag fully.

In order to facilitate the semen dilution, in step 4, a peristaltic pump is used to slowly inject the diluent into the dilution bag.

In order to facilitate the semen subpackaging, in step 5, 40ml-100ml of semen is slowly subpackaged into semen subpackaging bags by using a peristaltic pump.

Has the advantages that: the invention provides a method for collecting, diluting and subpackaging boar semen, wherein the semen is always in an air-isolated state in the whole processes of semen collection, dilution and subpackaging, the transfer process among a plurality of vessels is reduced, the time of the whole process is reduced, and the whole working efficiency is improved; meanwhile, bacterial contamination in the process of transferring semen between vessels is avoided, and compared with a glove-worn freehand semen collection method, the total number index of bacterial colonies is 10 times of the total number of bacterial colonies after semen collection, so that the survival rate and activity of the semen are guaranteed.

Drawings

Fig. 1 is a schematic structural diagram of this embodiment 1.

Reference numerals: 1. a semen collector; 2. a hose; 3. a semen collection kit; 4. semen collection bags; 5. a thermal insulation material; 6. a diluting bag; 7. a connecting port; 8. a sampling port.

Detailed Description

The following detailed description of the embodiments of the present invention will be described in detail with reference to the accompanying drawings, but the present invention is not limited to these embodiments, and modifications made to the invention without departing from the principle of the invention fall within the scope of the claims of the present invention.

Example 1

As shown in fig. 1, a device for collecting, diluting and separately packaging boar semen comprises a semen collector 1, a hose 2 and a semen collection sleeve box 3, wherein a semen collection bag 4 is arranged in the semen collector 1, the semen collection sleeve box 3 comprises a heat insulation material 5 and a dilution bag 6, the dilution bag 6 is wrapped by the heat insulation material 5 for heat insulation, a connection port 7 and a sampling port 8 are arranged on the dilution bag 6, the semen collection bag 4 is communicated with the connection port 7 on the dilution bag 6 through the hose 2 and used for guiding semen to the dilution bag 6, and the semen collection sleeve box 3 can be integrally and independently detached.

A method for collecting, diluting and subpackaging boar semen specifically comprises the following steps:

step 1, a boar is guided to insert a penis into the semen collector 1, and liners such as a heating sheet and silica gel are attached to the interior of the semen collector 1 to simulate the vaginal environment of the sow. The penis of the boar moves forward, and the front end of the boar becomes narrower. Because the spiral head at the front end of the penis of the boar is sensitive to pressure, the structure can stimulate the boar to produce excitation so as to start ejaculation. The boar finishes the whole ejaculation process in the semen collector 1, and the semen is guided to the diluent bag 6 through the hose 2. And when all the semen flows to the diluent bag 6, taking down the semen collection sleeve box 3 and delivering the semen to a laboratory technician through a delivery window. After the semen collection of one boar is finished, a technician can immediately replace the lining in the semen collector 1 and the semen collection sleeve box 3 to start the semen collection operation of the next boar. Because the semen collection sleeve 3 is a standard part and the quality change is within an acceptable error range, the weight of the semen can be obtained by weighing the semen collection sleeve 3 and subtracting the weight of the semen collection sleeve 3; since the relationship between the quality and volume of porcine semen is approximately equal to 1, and the error is within an acceptable range, we treat semen quality and volume as equivalent. After a technician in a laboratory takes the semen collection sleeve box 3, the semen amount is obtained by directly weighing the weight of the semen collection sleeve box 3.

Step 2, horizontally placing the semen collection sleeve box 3 on a mechanical arm, and uniformly rotating the mechanical arm at an angular speed of 180 DEG/s for 15 seconds to fully and uniformly mix the semen in the diluent bag 6;

step 3, sucking 0.5-1 ml of uniformly mixed original semen through a sampling port 8 on the semen collection sleeve box 3 for inspection, discarding unqualified semen, and diluting qualified semen;

and 4, after the semen is qualified through inspection, connecting the hose 2 with a connecting port 7 on the semen collection sleeve box 3, slowly injecting a certain amount of diluent through the hose 2 by using a peristaltic pump, and rotating the mechanical arm at an angular speed of 180 degrees/s for 30 seconds at a constant speed, so that the semen is fully and uniformly mixed in the diluent bag 6. The semen dilution multiple generally contains 30-40 hundred million effective sperms according to each semen-handling dose, the semen-handling capacity of an external boar is 80-100 ml, the semen-handling capacity of a local variety boar is 40-60 ml, the addition amount of the semen dilution is calculated according to the semen sampling check density and the motility rate, and the main variables are the effective semen number contained in the semen-handling dose and the semen-handling dose of each time;

assuming that the semen collection amount is A, the motility rate is B, the sperm density is C, the effective sperm number D of the semen is AxBxC, and the dilutable total part of the semen is E₁D/40 or E₂D/30, i.e. E is within the interval (D/40, D/30);

if the semen is used for the semen deposition of the exogenous pig, the semen dosage is 90ml, the total amount of the diluted semen is F-E multiplied by 90ml, and the addition amount of the semen diluent is G-F-A and G₁＝[90×(A×B×C)/40]-A or G₂＝[90×(A×B×C)/30]A, i.e., [ 90X (A X B X C)/40]-A＜G＜[90×(A×B×C)/30]-A；

② if the semen is used for the insemination of local variety sow, the semen dosage is 40-60 ml, then the total amount of diluted semen is F₁E × 40ml or F₂E × 60ml, i.e. F is within the interval (E × 40, E × 60), and the addition amount of semen diluent G ═ F-A, G₁＝[50×(A×B×C)/40]-A or G₂＝[50×(A×B×C)/30]A, i.e., [ 50X (A X B X C)/40]-A＜G＜[50×(A×B×C)/30]-A；

Considering 2 cases in total, the addition amount of semen diluent G should be in the range of [ 50X (A X B X C)/40] -A < G < [ 90X (A X B X C)/30] -A;

for example: the one-time semen collection amount of a certain male boar is 200ml, the survival rate is 80%, the sperm density is 3 hundred million/ml, the total number of semen sperms is 200ml multiplied by 3 hundred million/ml which is 600 hundred million, the total number of effective sperms is 600 hundred million multiplied by 80 percent which is 480 hundred million, each semen is counted by 40 hundred million effective sperms, the total number of dilutable semen is 480 hundred million multiplied by 40 hundred million which is 12 hundred million multiplied by 90 ml/1080 ml, the addition amount of semen diluent is 1080ml-200ml which is 880 ml; and 5, slowly pumping out 50ml or 90ml of semen by using a peristaltic pump, and automatically subpackaging into semen bags.

Comparative test

9 adult boars (4 Duroc pigs, 3 Changbai pigs and 2 York pigs) are fed according to the nutrition standard of breeding boars in a commercial boar station, and temperature control equipment such as a wet curtain, a fan and the like is adopted in a pigsty to ensure that the temperature in the pigsty is about 28 ℃. Before the test, all the boars receive the training of manual free-hand semen collection and automatic semen collection, each boar can alternately collect semen collection and glove-wearing free-hand semen collection on Monday and Monday, the test is half a year, the time when the boars enter a semen collection fence is taken as the semen collection starting time point, the boars leave the semen collection fence as the semen collection finishing time point, and the time when the semen split charging is finished is defined as the semen production finishing time point.

Semen collection time of boar is equal to semen collection ending time-semen collection starting time

The production time of the pig semen is equal to the completion time of the production of the semen-the starting time of the semen collection

After the two methods are finished, the semen quantity is detected, and the indexes such as the semen vitality, the density, the total bacterial colony number and the like are checked by sampling.

The related results are shown in the first, second, third and fourth tables (wherein 1, 2, 3 and 4 are Duroc pigs, 5, 6 and 7 are Changbai pigs, 8 and 9 are York pigs, and the following automatic semen collection method is used for replacing semen collection, dilution and subpackage method of pigs)

Watch-boar semen volume (ml)

Semen collection mode

1

2

3

4

5

6

7

8

9

Manual semen collection

246±12

267±15

273±16

253±13

235±14

227±18

250±17

237±12

215±13

Automatic semen collection method

236±11

251±16

248±14

234±12

213±13

217±16

231±14

214±15

220±14

Sperm density of male boar II (individual/ml)

Total sperm count of male boar semen (number)

TABLE four boar colony count (CFU/ml)

Represents significant difference between two groups (P < 0.05), and represents significant difference between two groups (P < 0.01)

In the first, second and third tables, it can be seen that the total number of colonies in the automatic semen collection method is significantly 10 times lower than the corresponding index of the glove-worn freehand semen collection method in terms of reducing bacterial contamination (see table four) under the condition that the indexes of semen volume, density, total sperm count and the like are basically consistent with those of the automatic semen collection method.

Semen collection time and semen production time of Tatarius boars

Represents significant difference between two groups (P < 0.05), and represents significant difference between two groups (P < 0.01)

As shown in table five, the free-hand semen collection method with gloves in the semen collection time of the boar is slightly higher than the automatic semen collection method, but the automatic semen collection method is significantly higher than the manual semen collection method in the number of the heads of the boar for semen collection per hour, when the automatic semen collection is used, a technician needs to guide the penis of the boar into a false vagina after climbing across a false female platform, and the semen collection box is transferred to a laboratory after the semen collection of the boar is completed. The automatic semen collection can drive the boars on the second day to enter the preparation fence in the semen collection process of the first boar, and the semen collection boars of the second boar are carried out immediately after the first boar is finished. And wear the bare-handed semen collection of gloves and need semen collection technical staff to last to hold the boar penis at semen collection in-process, not only consume great physical power and energy and do not have the clearance to carry out the preparation of next boar, because our comparison is gone on when single semen collection technical staff carries out semen collection operation, if when the semen collection strategy of a plurality of false mother platforms of many people is taken at large-scale boar station, help semen collection and drive under the condition of boar by 2 individuals, the efficiency of semen collection will increase at double in the unit interval.

In addition, the production time of the pig semen is about 10 minutes less for the automatic semen collection and automatic semen dilution subpackage system of each semen compared with the manual semen collection and the traditional subpackage (Table 5). The automatic sub-packaging system inputs semen density parameters into the system by workers, and does not need any operation after the outer tube is connected in the diluting and sub-packaging processes. Dilution and partial shipment are all in the dilution bag, accomplish the leading-in of diluent and the derivation of the semen after diluting by the peristaltic pump, and this in-process semen need not to expose the probability that can greatly reduce secondary pollution in the external world, is favorable to the semen to be preserved more. The device can greatly improve the semen production efficiency and the semen preservation effect in the larger-scale semen production.