阅读说明：本技术 一种抗菌组合物及其制备方法和应用 (Antibacterial composition and preparation method and application thereof ) 是由 蔡敬贤 桂红 陈红英 邓小英 于 2021-09-30 设计创作，主要内容包括：本发明属于化妆品技术领域,公开了一种抗菌组合物及其制备方法和应用。该抑菌组合物,包括植物提取物、酵母菌多肽类和1,2-己二醇；植物提取物为连翘提取物和花椒果提取物。本发明提供的抑菌组合物,通过连翘提取物、花椒果提取物、酵母菌多肽类和1,2-己二醇的配合使用,提高了组合物抗菌效果,能够代替传统化学防腐剂。且该抑菌组合物还具有调节皮肤微生物菌群,上调抗菌肽的表达,提升皮肤自身免疫能力,降低皮肤刺激性的功效。该抑菌组合物温和无刺激,与其他成分相容性好,可应用于水剂、乳液、膏霜以及啫喱中,其配伍性好。(The invention belongs to the technical field of cosmetics, and discloses an antibacterial composition, and a preparation method and application thereof. The antibacterial composition comprises plant extract, yeast polypeptide and 1, 2-hexanediol; the plant extract is fructus forsythiae extract and fructus Zanthoxyli extract. According to the antibacterial composition provided by the invention, the fructus forsythiae extract, the zanthoxylum bungeanum fruit extract, the saccharomycete polypeptide and the 1, 2-hexanediol are used in a matching manner, so that the antibacterial effect of the composition is improved, and the traditional chemical preservative can be replaced. The antibacterial composition also has the effects of regulating skin microbial flora, up-regulating the expression of antibacterial peptide, improving the skin autoimmune capacity and reducing skin irritation. The antibacterial composition is mild, has no irritation, has good compatibility with other components, can be applied to water aqua, lotion, cream and gel, and has good compatibility.)

1. A bacteriostatic composition, comprising plant extract, yeast polypeptides and 1, 2-hexanediol; the plant extract is fructus forsythiae extract and fructus Zanthoxyli extract.

2. The bacteriostatic composition according to claim 1, wherein the mass ratio of the forsythia suspense extract to the zanthoxylum bungeanum maxim extract is 1: (1-5); preferably, the mass ratio of the forsythia suspense extract to the zanthoxylum bungeanum maxim extract is 1: (1-2).

3. The bacteriostatic composition according to claim 1, which comprises 1-15 parts by weight of plant extract, 0.1-3 parts by weight of yeast polypeptide and 1-10 parts by weight of 1, 2-hexanediol.

4. Bacteriostatic composition according to claim 3, wherein the bacteriostatic composition comprises 2-12 parts by weight of plant extract, 0.15-2 parts by weight of yeast polypeptides and 2-8 parts by weight of 1, 2-hexanediol.

5. The bacteriostatic composition according to any one of claims 1 to 4, further comprising 0.05 to 2 parts by weight of arginine.

6. The bacteriostatic composition according to claim 5, which comprises 2 parts by weight of forsythia suspensa extract, 3 parts by weight of zanthoxylum bungeanum fruit extract, 0.5 part by weight of saccharomycete polypeptides, 2 parts by weight of 1, 2-hexanediol and 0.15 part by weight of arginine.

7. Use of a bacteriostatic composition according to any one of claims 1-6 in cosmetics.

8. A lotion, characterized by comprising the bacteriostatic composition according to any one of claims 1-6.

9. An emulsion comprising the bacteriostatic composition of any one of claims 1-6.

10. A cream comprising the bacteriostatic composition of any one of claims 1-6.

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to an antibacterial composition and a preparation method and application thereof.

Background

In the cosmetic field, preservation of cosmetics is of vital importance, mainly involving the use of traditional chemical preservatives and, in recent years, preservative-free systems.

Although the traditional chemical preservative has strong bactericidal capacity, after cosmetics of the traditional chemical preservative are used for a long time, the traditional chemical preservative can destroy and inhibit resident flora of the skin, disturb the microecological balance of the skin, damage the skin barrier and reduce the immunity, thereby causing various skin problems.

In recent years, there have been many researches on non-anticorrosion systems, and there are two main non-anticorrosion systems currently used in the market: the first one is preservative system compounded with polyol, acid, ester, ketone, etc. and the preservative system includes mainly caprylyl glycol, caprylyl hydroximic acid, caprylic glyceride, caprylic sorbitol ester, p-hydroxy acetophenone, etc. The advantages are that the moisturizing effect can be increased for the formula, but the disadvantages are that the smell is not good, the consumer is not easy to accept, and the burning and irritating feeling can be generated when the skin contacts too much polyhydric alcohol; in addition, the antibacterial broad spectrum is poor, and the compatibility in the formula has certain limitation. The other is a part of plant bacteriostatic agent and microbial bacteriostatic agent, which mainly comprises fennel, orange, white willow, cinnamon, lysozyme and the like. The method has the advantages that the method can meet the requirements of partial consumers without adding essence and has certain inhibition effect on microbial flora; the disadvantages of the method are difficult to be applied to aqueous formulations, large limitation and high cost. The two common non-antiseptic systems have limitations in antiseptic and cannot provide additional efficacy for cosmetics, and the effects are single.

Therefore, there is a need to provide an antibacterial composition that can effectively inhibit bacteria, replacing traditional chemical preservatives; and can provide additional efficacy to the cosmetic and aid in skin care.

Disclosure of Invention

The present invention is directed to solving at least one of the problems of the prior art described above. Therefore, the invention provides an antibacterial composition which has obvious antibacterial effect on bacteria, saccharomycetes, mould and the like and can replace the traditional chemical preservative; the antibacterial composition also has the effects of regulating skin micro-ecology, promoting skin lipid synthesis, regulating skin autoimmune capability and reducing skin irritation.

In a first aspect, the present invention provides an antimicrobial composition.

Specifically, the bacteriostatic composition comprises a plant extract, saccharomycete polypeptides and 1, 2-hexanediol; the plant extract is fructus forsythiae extract and fructus Zanthoxyli extract.

The antibacterial effect of the composition is improved by the matching use of the forsythia suspense extract, the zanthoxylum bungeanum maxim extract, the saccharomycete polypeptide and the 1, 2-hexanediol, wherein active ingredients such as limonene, alkenes, alcohols, saponin, hypericin and the like contained in the forsythia suspense extract and the zanthoxylum bungeanum maxim extract can penetrate through a cell membrane structure to interfere the physiological activity of microorganisms, so that the proteins are denatured, and the antibacterial effect is achieved; the forsythia extract, the zanthoxylum bungeanum fruit extract, the saccharomycete polypeptide and the 1, 2-hexanediol have obvious bacteriostatic effects on heat-resistant coliform bacteria, pseudomonas aeruginosa, candida albicans, aspergillus niger and staphylococcus aureus under the combined action. Meanwhile, the composition also has the effects of regulating skin microbial flora, up-regulating the expression of antibacterial peptide, improving the skin autoimmune capability and reducing the skin irritation.

Preferably, the mass ratio of the forsythia suspense extract to the zanthoxylum bungeanum maxim extract is 1: (1-5); further preferably, the mass ratio of the forsythia suspensa extract to the zanthoxylum bungeanum maxim extract is 1: (1-3); more preferably, the mass ratio of the forsythia suspensa extract to the zanthoxylum bungeanum fruit extract is 1: (1-2). By controlling the dosage ratio of the forsythia suspense extract to the zanthoxylum bungeanum maxim extract, the bacteriostatic effect of the bacteriostatic composition can be improved, and the skin microbial flora can be better regulated.

Preferably, the bacteriostatic composition comprises 1-15 parts by weight of plant extract, 0.1-3 parts by weight of saccharomycete polypeptide and 1-10 parts by weight of 1, 2-hexanediol; further preferably, the bacteriostatic composition comprises 2-12 parts of plant extract, 0.3-2 parts of saccharomycete polypeptide and 2-8 parts of 1, 2-hexanediol; more preferably, the bacteriostatic composition comprises 2-8 parts of plant extract, 0.3-1 part of saccharomycete polypeptide and 2-5 parts of 1, 2-hexanediol.

Preferably, the bacteriostatic composition further comprises arginine. The arginine is capable of adjusting the pH of the composition or cosmetic system; on the other hand, the arginine can also promote the effects of saccharomycete polypeptides, forsythia suspense extract and zanthoxylum bungeanum fruit extract, is favorable for regulating skin microbial flora and up-regulating the expression of the antibacterial peptide.

Preferably, the arginine accounts for 0.05 to 2 parts by weight; further preferably, the arginine is 0.1-1 part; more preferably, the arginine is 0.1-0.5 parts.

Preferably, the antibacterial composition comprises 2 parts of fructus forsythiae extract, 3 parts of zanthoxylum bungeanum fruit extract, 0.5 part of saccharomycete polypeptide, 2 parts of 1, 2-hexanediol and 0.15 part of arginine in parts by weight.

In a second aspect, the present invention provides a method for preparing an antimicrobial composition.

Specifically, the preparation method of the antibacterial composition comprises the following steps: and mixing the components to obtain the antibacterial composition.

The bacteriostatic composition can be dissolved by adding a solvent according to needs in the preparation process, for example, water is used as the solvent for dissolution. There is no requirement for the amount of water used.

Preferably, when water is used as the solvent, the preparation method comprises the following steps:

(1) controlling the water temperature to be 45-50 ℃, adding the fructus forsythiae extract and the fructus zanthoxyli extract, stirring and dissolving until the fructus forsythiae extract and the fructus zanthoxyli extract are transparent, and cooling to room temperature to obtain a solution A;

(2) adding the yeast polypeptide and the 1, 2-hexanediol into the solution A under the condition of stirring, and mixing to obtain a solution B;

(3) adding the arginine into the solution B, mixing, adjusting the pH value to 6.0-6.5, and filtering to obtain the antibacterial composition.

In a third aspect, the present invention provides the use of an antimicrobial composition.

In particular to the application of the antibacterial composition in cosmetics.

Preferably, the cosmetic is one of a water agent, an emulsion, a cream or a gel.

Preferably, the cosmetic water comprises the antibacterial composition.

Preferably, the lotion further comprises at least one of an emulsifier, a humectant, or an antioxidant.

Preferably, an emulsion comprises the antibacterial composition.

Preferably, the emulsion further comprises at least one of an emulsifier, a humectant, a thickener, or an antioxidant.

Preferably, a cream comprises the antibacterial composition.

Preferably, the emulsion further comprises at least one of an emulsifier, a chelating agent, a humectant, a thickener, or an antioxidant.

Compared with the prior art, the invention has the following beneficial effects:

(1) according to the antibacterial composition provided by the invention, the fructus forsythiae extract, the zanthoxylum bungeanum fruit extract, the saccharomycete polypeptide and the 1, 2-hexanediol are used in a matching manner, so that the antibacterial effect of the composition is improved, the antibacterial composition has a remarkable antibacterial effect on heat-resistant coliform, pseudomonas aeruginosa, candida albicans, aspergillus niger and staphylococcus aureus, and can replace the traditional chemical preservative.

(2) The antibacterial composition provided by the invention also has the effects of regulating the microbial flora of the skin, up-regulating the expression of the antibacterial peptide, improving the autoimmune capacity of the skin and reducing the skin irritation.

(3) The antibacterial composition provided by the invention is mild and non-irritant, has good compatibility with other components, can be applied to water aqua, emulsion, cream and gel, and has good compatibility.

Detailed Description

In order to make the technical solutions of the present invention more apparent to those skilled in the art, the following examples are given for illustration. It should be noted that the following examples are not intended to limit the scope of the claimed invention.

In the following examples or comparative examples, the forsythia suspense extract and zanthoxylum bungeanum fruit extract were purchased from guangzhou general seafood fine chemical company, ltd, and the yeast polypeptides were purchased from guangzhou famous picture company, ltd. Other starting materials, reagents or equipment may be obtained from conventional commercial sources or may be obtained by known methods in the art, unless otherwise specified.

Examples 1 to 5

The components of the bacteriostatic compositions provided in examples 1-5 are shown in Table 1.

TABLE 1

Components	Example 1	Example 2	Example 3	Example 4	Example 5
						Forsythia suspensa extract	3.2	2	2.4	1	2
Zanthoxylum bungeanum fruit extract	4.8	3	3.6	4	3
						Yeast polypeptides	0.05	0.5	0.1	0.5	0.5
1, 2-hexanediol	1	2	5	2	2.15
						Arginine	0.2	0.15	0.2	0.15	0
Water (W)	90.75	92.35	88.7	92.35	92.35

Examples 1-5 provide a method of preparing a bacteriostatic composition, comprising the steps of:

(1) controlling water temperature at 45-50 deg.C, adding fructus forsythiae extract and fructus Zanthoxyli extract, stirring to dissolve until it is transparent, and cooling to room temperature to obtain solution A;

(2) adding the saccharomycete polypeptide and 1, 2-hexanediol into the solution A under the condition of stirring, and mixing to obtain a solution B;

(3) adding arginine into the solution B, mixing, adjusting the pH value to 6.0, and filtering to obtain the antibacterial composition.

Example 6

The components of an emulsion are shown in Table 2.

TABLE 2

Example 7

A cosmetic lotion has the composition shown in Table 3.

TABLE 3

Serial number	Standard Chinese name	The formula accounts for percent
			1	Deionized water	Balance of
2	Hyaluronic acid sodium salt	0.05
			3	EDTA 2Na	0.05
4	Allantoin	0.1
			5	1, 3-butanediol	8
6	Bacteriostatic composition prepared in example 2	10

Example 8

A facial cream has the components shown in Table 4.

TABLE 4

Phase difference	Standard Chinese name	The formula accounts for percent
			Oil phase	Pentaerythritol tetra (ethylhexanoate)	8
Oil phase	Polydimethylsiloxane	5
			Oil phase	Cetostearyl alcohol	2
Oil phase	Tocopherol acetate	1
			Oil phase	Steareth-21	2
Oil phase	Steareth-2	1.5
			Aqueous phase	Deionized water	Balance of
Aqueous phase	1, 3-butanediol	5
			Aqueous phase	Xanthan gum	0.2
Aqueous phase	Carbomer	0.1
				Arginine	0.08
	Bacteriostatic composition prepared in example 2	15

Comparative examples 1 to 4

The components of the bacteriostatic compositions provided in comparative examples 1 to 4 are shown in table 5.

TABLE 5

Components	Comparative example 1	Comparative example 2	Comparative example 3	Comparative example 4
					Forsythia suspensa extract	0	5	2	2
Zanthoxylum bungeanum fruit extract	5	0	3	4
					Yeast polypeptides	0.5	0.5	0	1.5
1, 2-hexanediol	2	2	2.5	0
					Arginine	0.15	0.15	0.15	0.15
Water (W)	92.35	92.35	92.35	92.35

The preparation method of the bacteriostatic composition provided by the comparative examples 1-4 comprises the following steps:

(1) controlling water temperature at 45-50 deg.C, adding fructus forsythiae extract and/or fructus Zanthoxyli extract, stirring for dissolving to transparent, and cooling to room temperature to obtain solution A;

(2) adding the saccharomycete polypeptide and/or 1, 2-hexanediol into the solution A under the condition of stirring, and mixing to obtain a solution B;

(3) adding arginine into the solution B, mixing, adjusting the pH value to 6.0, and filtering to obtain the antibacterial composition.

Comparative example 5

Comparative example 5 is different from example 6 in that the bacteriostatic composition prepared in example 2 was replaced with the bacteriostatic composition prepared in comparative example 1, and the rest of the components and the preparation method were the same as example 6.

Product effectiveness testing

(1) Testing the bacteriostatic Properties of the compositions

The experimental method comprises the following steps: the bacteriostatic compositions of examples 1-5 and comparative examples 1-4 were diluted to 10% aqueous solutions, respectively. Referring to a method for testing the microbial preservative efficacy in United states Pharmacopeia USP51, the standard numbered strains are: staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger were inoculated into the aqueous solutions of examples 1 to 5 and comparative examples 1 to 4, respectively, and then the number of survivors of the total number of colonies was examined on days 7, 14, and 28, respectively, and the logarithmic decrease of the total number of colonies was calculated, and the specific test results are shown in Table 6.

TABLE 6 results of the bacteriostatic test

The log reduction of the total number of colonies is lg (initial colony count/colony count on day X), and is specified in the evaluation standard: the log reduction of bacteria on day 14 of inoculation was greater than or equal to 2, no increase was observed on day 28 (no more than 0.5 increase from the previous measurement), and no increase was observed on days 14 and 28 for both yeast and mold inoculations.

As can be seen from Table 6, the combination of 1, 2-hexanediol and the yeast polypeptides with fructus forsythiae extract and fructus Zanthoxyli extract in examples 1-5 can provide a composition with good antibacterial effect. From example 5, it is clear that the addition of arginine not only can adjust the pH, but also can improve the bacteriostatic activity of the composition. The comparative examples 1 to 4 show that when any one of the forsythia suspense extract, the zanthoxylum bungeanum maxim extract, the 1, 2-hexanediol or the saccharomycete polypeptide is reduced, a good antibacterial effect cannot be achieved. Therefore, the saccharomycete polypeptides have synergistic effect on the preservative system of the forsythia suspensa extract, the zanthoxylum bungeanum fruit extract and the 1, 2-hexanediol.

(2) Testing the Effect of the bacteriostatic composition on skin Immunity

Experiments to verify that expression of antimicrobial peptides can be upregulated were performed using examples 1-5.

Culturing normal human epidermal keratinocytes, taking cells in logarithmic growth phase for experiment, and dividing the cells into 10 groups and blank groups: culture solution without test sample; test groups: culture solutions containing the bacteriostatic compositions of examples 1-5, each at a concentration of 20. mu.g/mL, for 24 h.

Antibacterial peptides are an important part of the body's natural immunity, and Cathelicidin LL37 is one of the most common types of antibacterial peptides found on human skin. Based on the experimental method, cell supernatant is collected, the content of Cathelicidin LL37mRNA is expressed by the ratio of the IOD value of Cathelicidin LL37 product to the IOD value of beta-actin, and higher ratio indicates higher content of Cathelicidin LL 37; the amount of Cathelicidin LL37 protein was expressed as the ratio of Cathelicidin LL37 to β -actin grey scale values to examine the expression of Cathelicidin LL37mRNA and protein in keratinocytes. The experiment was repeated 3 times and averaged, and the results are shown in table 7.

TABLE 7 expression of Cathelicidin LL37mRNA and protein

	Cathelicidin LL37/β-actin mRNA	Cathelicidin LL 37/beta-actin protein
			Blank group	0.314±0.027	0.229±0.036
Example 1	0.391±0.023	0.401±0.015
			Example 2	0.492±0.058	0.462±0.074
Example 3	0.363±0.042	0.374±0.035
			Example 4	0.357±0.022	0.347±0.024
Example 5	0.373±0.014	0.381±0.011

As can be seen from table 7, the bacteriostatic composition provided by the invention can up-regulate expression of Cathelicidin LL37mRNA and protein, and particularly, the bacteriostatic composition of example 2 has the best effect of up-regulating expression of antibacterial peptide.

(3) Testing anti-inflammatory efficacy of bacteriostatic compositions

The bacteriostatic compositions of examples 1-5 were used for anti-inflammatory efficacy testing, as follows:

the experimental method comprises the following steps: lipopolysaccharide (LPS) is adopted to stimulate macrophage Raw264.7 to establish an inflammation model, and after the sample is detected to act, the content change of related inflammatory factors (IL-1 alpha and PGE2) is measured to evaluate the anti-inflammatory efficacy of the sample.

Cell inoculation: by 6.4X 10⁵(ii) inoculation Density of one/well macrophages (mouse macrophages) were inoculated into 6-well plates, incubators (37 ℃, 5% CO)₂) Incubating overnight;

preparing liquid: test solutions were prepared according to the experimental design, blank group: culture solution without test sample and LPS stimulation; negative control group: culture solution without LPS stimulation of test sample; positive control group: a culture solution containing dexamethasone (with the concentration of 100 mu g/mL); test groups: culture broth containing examples 1-5 (10% concentration); administration: when the cell plating rate in the 6-hole plate reaches 40% -60%, the medicine is administered in groups, the dosage of each hole is 1.8mL, each group is provided with 3 parallel, and the incubator (37 ℃, 5% CO)₂) Continuously culturing for 2 h;

stimulation by LPS: after 2h incubation, 200uL LPS working solution prepared from the corresponding test substance was added to the dosed well plates, and the wells were shaken from side to sidePlate, mix well the drugs in the well plate, LPS final concentration 1. mu.g/mL, incubator (37 ℃, 5% CO)₂) Continuing to culture for 22 h;

collecting a sample: after the incubation is finished, collecting cell culture solution supernatant in an EP tube, and after the collection, placing the sample in a refrigerator at-80 ℃ for freezing and storing;

detection of IL-1. alpha. content: detecting according to the operation instruction of the Mouse IL-alpha ELISA kit; detection of the content of PGE 2: detection was performed according to the instructions for the PGE2 ELISA kit. The test results are shown in Table 8.

TABLE 8 content of IL-1. alpha. and PGE2, inflammatory factors related thereto

	IL-1. alpha. content (pg/mL)	PGE2 content (pg/mL)
			Blank group	10.2	2000
Negative control group	83.5	34000
			Positive control group	13.2	21000
Example 1	42.0	25000
			Example 2	24.5	22000
Example 3	28.3	23000
			Example 4	56.4	27000
Example 5	45.2	25000

As shown in Table 8, compared with the blank group, the secretion of macrophage inflammatory factor IL-alpha of the negative control group is obviously increased, and the secretion of macrophage inflammatory factor IL-alpha of the positive control group is obviously reduced under the administration concentration of dexamethasone of 100 mu g/mL, which indicates that the LPS stimulation condition and the experiment of the experiment are effective. When the bacteriostatic composition containing the components in examples 1-5 is administered at a concentration of 10%, the secretion amount of macrophage inflammatory factor IL-alpha is reduced.

Compared with a blank group, the secretion of macrophage inflammatory mediator PGE2 in a negative control group is obviously increased, and the secretion of macrophage inflammatory mediator PGE2 in a positive control group is obviously reduced under the administration concentration of dexamethasone 100 mu g/mL, so that the LPS stimulation condition and the experiment in the experiment are effective. When the bacteriostatic composition containing the examples 1-5 is administered, the secretion amount of PGE2 as macrophage inflammatory medium shows a decreasing trend.

Therefore, the antibacterial composition compounded by the forsythia suspense extract, the zanthoxylum bungeanum fruit extract, the 1, 2-hexanediol and the saccharomycete polypeptide can reduce the irritation and sensitivity of the skin, and the result proves that the antibacterial composition provided by the invention obviously enhances the capability of maintaining the homeostasis of the skin and enhances the immunity, so that the comfortable feeling of the skin can be improved.

(4) Testing the efficacy of the bacteriostatic composition in repairing the skin barrier

The experiments were divided into two groups, test group: the emulsion provided in example 6 (test sample) was used; control group: human testing was performed using the emulsion provided in comparative example 5 (control sample) using a test method highly similar to that in real life, and TEWL value, hydration, dryness evaluations were performed to evaluate the efficacy of the sample tested, with the following specific test methods:

58 subjects with very dry skin were selected, randomized into two groups, the forearm flexor was selected, the forearm was cleaned twice daily at home with the same brand of soap during the 14 day wash period, the rate of change of TEWL value (loss of moisture through the skin), hydration, dryness assessment of the test skin were collected and recorded as baseline using a dermab Combo skin tester (purchased from CORTEX tecnology, denmark), and the product was used 2 times daily at home during the experimental treatment period. In the first group, a test sample is smeared on a left arm, and is smeared on a right arm in the same amount by adopting clear water; the second group applied the control sample to the left arm and the same amount to the right arm with clear water. The forearms were washed once daily, evaluated on days 7, 14, 28, 42, and then cleaned twice daily with household soap for another 3 days, evaluated on day 45, and compared to before the start of the test, with the test results shown in table 9, table 10, table 11.

The TEWL value represents the amount of percutaneous water loss in unit time and unit area, once the barrier function of the skin is slightly damaged, even damage which cannot be observed by naked eyes, the percutaneous water loss is increased, and the higher the TEWL value reduction rate is, the better the repairing effect of the sample is; the capacitance integral reflects the skin compactness and the moisture preservation capacity, and the higher the rise rate of the capacitance integral is, the better the moisture preservation capacity of the skin is; and the dryness evaluation is to evaluate the change of the skin dryness on the whole, and the higher the reduction rate is, the better the capability of the sample for improving the skin dryness is.

TABLE 9 TEWL variation rate

TABLE 10 rate of change of capacitance integral value

TABLE 11 Dry score

Note: "+" indicates < 0.05, "+" indicates < 0.01, "+" indicates P < 0.001.

As can be seen from tables 9-10, as time goes by, the TEWL values of the control group and the test group both decrease, the initial values decrease very obviously from day 14, the capacitance integrals of the control group and the test group both increase, and the initial values increase very obviously from day 14, but the effect of the test group is obviously better than that of the control group. It can be seen that the test group contained the composition of example 2 which significantly improved the skin barrier integrity and enhanced the skin moisturizing ability from day 7 to day 42.

As can be seen from the data in Table 11, the decrease of skin dryness was very significant after 3 days on the skin which was very dry after the treatment, indicating that the test group could continuously relieve the dryness of the skin, and the effect was significantly better than the control group, indicating that the skin barrier was broken and the skin defense was weakened after the use was effectively improved.