阅读说明：本技术 富钒水溶液在解酒中的应用 (Application of vanadium-rich aqueous solution in relieving alcoholism ) 是由 王越 高誉欣 王逸凡 孙雯 于 2021-08-20 设计创作，主要内容包括：本发明涉及医药技术领域,具体涉及富钒水溶液在解酒中的应用。本发明通过发现了富钒水溶液具有较好的解酒功能,不仅可有效缓解酒精导致的共济失调,还能缩短醉酒状态持续的时间,并且在长期饮用富钒水溶液的基础上,于酒后一次性再加饮该富钒水溶液解酒效果更好,为解酒产品的研发提供了新的思路。(The invention relates to the technical field of medicines, in particular to application of a vanadium-rich aqueous solution in relieving alcoholism. According to the invention, the vanadium-rich aqueous solution has a good hangover alleviating function, not only can ataxia caused by alcohol be effectively relieved, but also the duration time of a drunk state can be shortened, and on the basis of drinking the vanadium-rich aqueous solution for a long time, the vanadium-rich aqueous solution is drunk once after drinking for a better hangover alleviating effect, so that a new thought is provided for the research and development of hangover alleviating products.)

1. An application of vanadium-rich water solution in preparing hangover relieving product is provided.

2. The use of claim 1, wherein the anti-hangover effect is manifested by at least:

1) relieving ataxia caused by alcohol;

2) reducing the duration of disappearance of the righting reflex;

3) shortening the duration of the drunk state.

3. An anti-alcohol product is characterized in that the anti-alcohol product is a vanadium-rich aqueous solution, and the content of vanadium in the vanadium-rich aqueous solution is 0.01-2 mg/L.

4. The anti-hangover product according to claim 3, which is a food or a pharmaceutical; further, the food is a health food.

5. The anti-hangover product according to claim 4, wherein the drinking dose of the anti-hangover product is from 0.0002 to 0.04 mg/kg; preferably, the dosage of the vanadium-rich water is 0.001mg/kg, 0.002mg/kg, 0.004mg/kg and 0.01 mg/kg.

6. A pharmaceutical preparation comprising the anti-hangover product according to claim 4 and one or more pharmaceutically or dietetically acceptable excipients.

7. The pharmaceutical preparation according to claim 6, wherein the pharmaceutically or dietetically acceptable excipients are carriers, excipients and diluents commonly used in pharmacy or dietetics.

8. The pharmaceutical formulation of claim 7, wherein the carriers, excipients and diluents include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate and mineral oil.

9. The pharmaceutical formulation according to claim 6, wherein the subject to which the pharmaceutical formulation is administered is a human or non-human mammal, such as a mouse, rat, guinea pig, rabbit, dog, monkey, orangutan.

10. The application of the vanadium-rich water in the preparation of any one or more of the following products:

1) products for relief of ataxia caused by alcohol;

2) a product that reduces the duration of disappearance of the righting reflex;

3) a product for shortening the duration of an intoxicated state.

Technical Field

The invention relates to the technical field of medicines, in particular to application of a vanadium-rich aqueous solution in relieving alcoholism.

Background

The information in this background section is only for enhancement of understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.

Mineral substances such as trace element vanadium and the like are rich in underground water collected in Fuji mountains and Jizhou islands of Korea, and a large number of documents report that the frequent drinking of the vanadium-rich water can reduce blood sugar by changing hepatic fat catabolism, glucose phosphorylation process and the like, thereby avoiding diseases such as hyperglycemia and the like and being beneficial to the health of people. The underground water found in Laxi area of Shandong province in China is also rich in minerals such as vanadium, strontium and the like through detection, and has proved to have better hypoglycemic effect. Besides the hypoglycemic effect, whether drinking the vanadium-rich groundwater has other biological effects is still unknown.

Acute alcoholism refers to a state in which the central nervous system is changed from excitation to inhibition due to excessive drinking, drunk people often have the characteristics of unclear mouth and teeth and uncoordinated limbs, and severe people can cause diseases such as acute gastrorrhagia, acute hepatitis, hypertension and the like, and even die. The appearance of intoxicated states is associated with the cytotoxicity of ethanol during metabolism. Therefore, the research and development of the anti-alcohol product have good application prospect.

Disclosure of Invention

Aiming at the problems in the prior art, the invention aims to provide the application of the vanadium-rich aqueous solution in relieving alcoholism. According to the research of the invention, the vanadium-rich aqueous solution has a good anti-alcoholism function, not only can the ataxia caused by alcohol be effectively relieved, but also the duration time of the drunk state can be shortened, and on the basis of drinking the vanadium-rich aqueous solution for a long time, the effect of anti-alcoholism by drinking the vanadium-rich aqueous solution once after drinking is better.

In order to achieve the above object, the technical solution of the present invention is as follows:

in a first aspect of the invention, the application of a vanadium-rich aqueous solution in preparing an anti-hangover product is provided.

In a second aspect of the invention, the hangover alleviating product is a vanadium-rich aqueous solution, and the content of vanadium in the vanadium-rich aqueous solution is 0.01-2 mg/L.

In a third aspect of the invention, a pharmaceutical preparation is provided, which comprises the above-mentioned anti-hangover product and one or more pharmaceutically or dietetically acceptable excipients.

The specific embodiment of the invention has the following beneficial effects:

according to the invention, the vanadium-rich aqueous solution has a good hangover alleviating function, not only can ataxia caused by alcohol be effectively relieved, but also the duration time of a drunk state can be shortened, and on the basis of drinking the vanadium-rich aqueous solution for a long time, the vanadium-rich aqueous solution is drunk once after drinking for a better hangover alleviating effect, so that a new thought is provided for the research and development of hangover alleviating products.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.

FIG. 1 is a schematic diagram of the drop latency of mice in an acute treatment group given vanadium-rich water according to an embodiment of the present invention;

FIG. 2 is a graph showing the drop latency of mice administered to a chronic vanadium-rich water treatment group according to an embodiment of the present invention;

FIG. 3 is a schematic temperature chart of mice in an acute treatment group given vanadium-rich water according to an embodiment of the present invention;

FIG. 4 is a graph showing the body temperature of mice in a chronic treatment group administered with vanadium-rich water according to an embodiment of the present invention;

FIG. 5 is a schematic diagram showing the incubation period of disappearance of righting reflex in mice given to the vanadium-rich water acute treatment group in the example of the present invention;

FIG. 6 is a schematic diagram showing the duration of disappearance of righting reflex given to mice in the vanadium-rich water acute treatment group according to the example of the present invention;

FIG. 7 is a graph showing the incubation period for disappearance of the righting reflex in mice administered with the vanadium-rich water in the chronic treatment group according to the example of the present invention;

FIG. 8 is a graph showing the duration of disappearance of righting reflex in mice administered with the vanadium-rich water in the chronic treatment group according to the example of the present invention;

FIG. 9 shows a combination experiment of an embodiment of the present invention: schematic diagram of duration of disappearance of righting reflex of mice treated with combination of vanadium-rich water and acute and chronic conditions.

Detailed Description

It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.

In one embodiment of the invention, an application of a vanadium-rich aqueous solution in preparing an anti-hangover product is provided.

Specifically, the hangover alleviating effect is at least as follows:

1) relieving ataxia caused by alcohol;

2) reducing the duration of disappearance of the righting reflex;

3) shortening the duration of the drunk state.

In a second aspect of the invention, the hangover alleviating product is a vanadium-rich aqueous solution, and the content of vanadium in the vanadium-rich aqueous solution is 0.01-2 mg/L.

In another embodiment of the present invention, the anti-hangover product is a food or a medicine; further, the food is a health food.

In still another embodiment of the present invention, for chronic experiments on mice, the amount of the vanadium-rich aqueous solution consumed freely per day is 0.002-0.4 mg/kg; further preferably, the daily dosage of the vanadium-rich water is 0.01mg/kg, 0.02mg/kg, 0.04mg/kg and 0.1 mg/kg. According to the dose conversion relation between human and mouse, the dose of the vanadium-rich water is presumed to be 0.0002-0.04mg/kg for human; further preferably, the dosage of the vanadium-rich water is 0.001mg/kg, 0.002mg/kg, 0.004mg/kg and 0.01 mg/kg. The amount of the vanadium-rich water solution for intragastric administration in the acute experiment process is 0.001-0.4mg/kg, and more preferably, the disposable dosage of the vanadium-rich water is 0.01mg/kg, 0.02mg/kg, 0.04mg/kg, 0.1mg/kg, and the dosage of the vanadium-rich water is presumed to be 0.001mg/kg, 0.002mg/kg, 0.004mg/kg, 0.01mg/kg for human beings.

In one embodiment of the invention, the invention provides a pharmaceutical preparation, which consists of the anti-alcoholism product and one or more pharmaceutically or dietetically acceptable auxiliary materials.

The pharmaceutically or dietetically acceptable excipient may be a carrier, excipient, diluent, etc. generally used in pharmacy or dietetical science. Further, the composition can be prepared into oral preparations such as powder, granule, tablet, capsule, suspension, emulsion, syrup, and spray according to a conventional method.

Such pharmaceutically inactive ingredients, which may include carriers, excipients and diluents, are well known in the art and can be determined by one of ordinary skill in the art to meet clinical criteria.

In still another embodiment of the present invention, the carrier, excipient and diluent include, but are not limited to, lactose, glucose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methyl hydroxybenzoate, propyl hydroxybenzoate, talc, magnesium stearate, mineral oil, and the like.

In still another embodiment of the present invention, the subject to which the pharmaceutical preparation is administered may be human and non-human mammals, such as mice, rats, guinea pigs, rabbits, dogs, monkeys, chimpanzees, and the like.

In yet another embodiment of the invention, there is provided the use of vanadium-rich water in the preparation of any one or more of the following:

1) products for relief of ataxia caused by alcohol;

2) a product that reduces the duration of disappearance of the righting reflex;

3) a product for shortening the duration of an intoxicated state.

The invention is further illustrated by the following examples, which are not to be construed as limiting the invention thereto. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

Examples

Study subjects: c57BL6J adult male mice, 25-30 g; feeding in SPF level environment, feeding in dark environment for 12h under light and 12h, and freely taking feed and drinking water.

Evaluation of drunk behavior of animals:

rotarod test-detection of ataxia

The speed of rotation was from 8rpm/min to 40 rpm/min and the time taken for the mice to fall off the rotarod was recorded as latency, up to 5 min. On the first day, adaptive training was performed, and the time for 3 mice to fall off the rod rotating apparatus was recorded, and the average was taken at intervals of 30s each time. The test was performed three times the next day, the average of the latter two tests was taken as the pre-alcohol value and the abdominal cavity was injected with 1.75g/kg ethanol (20% v/v) 30min later. Three more measurements are taken 30min after injection, and the average of the two latter measurements is taken as the post-alcohol value.

Acute experiment: the stomach was gavaged once with Double Distilled Water (DDW) or vanadium-rich water (HVW, 0.02mg/kg) 15min after ethanol injection, and the bar-rotating behavior test was performed 15min later.

Chronic experiments: mice were fed with Double Distilled Water (DDW) or vanadium-rich water (HVW, 0.02mg/kg) for 2 weeks, injected with ethanol, and tested for rod rotation behavior after 30 min.

As can be seen from fig. 1, the acute experiment: the drop latency of the mice given to the vanadium-rich water acute treatment group is obviously prolonged compared with that of the double distilled water group.

As can be seen from fig. 2, the chronic experiment: the drop latency of mice given to the vanadium-rich water chronic treatment group is obviously prolonged compared with that of the double distilled water group.

It can thus be seen that either acute or chronic administration of vanadium-enriched water can significantly alleviate ataxia caused by alcohol.

Low-temperature experiment:

animals were removed from the cages, weighed, and a veterinary clinical thermometer inserted into the rectum 1.5 cm for basal core body temperature testing until a stable reading was obtained. Immediately thereafter, 3.0g/kg of ethanol (20% v/v, prepared with 0.9% physiological saline) was intraperitoneally injected, and the temperature was measured 30, 60, 90, and 120min later.

Acute experiment: performing acute double-distilled water or vanadium-rich water intragastric administration once 15min after ethanol injection; chronic experiments: after the mice were raised for 2 weeks with double distilled water or vanadium-rich water, respectively, ethanol was injected, and body temperature measurement was started 30min later.

As can be seen from fig. 3, the acute experiment: the body temperature of the mice in the double distilled water group is obviously reduced after the alcohol treatment, the body temperature of the mice in the double distilled water group is increased when the vanadium-rich water is subjected to acute treatment, but the body temperature of the mice in the double distilled water group at the same time point is not statistically different.

As can be seen from fig. 4, the chronic experiment: the body temperature of the mice in the double distilled water group is obviously reduced after the alcohol treatment, and the body temperature reduction is not influenced by the long-term chronic treatment of the vanadium-rich water.

It can thus be seen that the temperature drop of the alcohol is not affected by either acute or chronic administration of the vanadium-rich water.

Righting reflection experiment:

20% ethanol is injected into the abdominal cavity, 4g/kg, and the positive reflex test is carried out. Mice were supine as not flipped for 30s indicating that the reflex disappeared. Two indices were recorded: 1. incubation period: time from alcohol injection to disappearance of righting reflex; 2. duration: the time until the righting reflex disappears to recover again. Acute experiment: performing intragastric administration with double distilled water or vanadium-rich water 30min after ethanol injection; chronic experiments: after the mice are respectively fed with double distilled water or vanadium-rich water for 2 weeks, the behavior change is observed after the injection of ethanol; combined gavage experiment: mice were raised for 2 weeks with double distilled water or vanadium-rich water, respectively, and then observed for behavioral changes after injection of ethanol. After 30min of alcohol injection, the stomach is irrigated once with double distilled water or vanadium-rich water, and whether the time for restoring the righting reflex is changed or not is observed.

As can be seen from fig. 5 and 6, the acute experiment: the incubation period and duration of disappearance of righting reflex of mice given the acute treatment of the vanadium-rich water were not significantly different from those of the double distilled water group.

As can be seen from fig. 7 and 8, the chronic experiment: the incubation period of disappearance of righting reflex of mice given the chronic treatment of the vanadium-rich water is not obviously different from that of the double distilled water group, but the duration of disappearance of righting reflex of the mice can be shortened.

Combination experiment: as can be seen from fig. 9, the duration of disappearance of righting reflex in the mice treated with the combination of the vanadium-rich water and the water was significantly shorter than that in the double distilled water group.

Therefore, it can be seen that acute administration of the vanadium-rich water after drinking can not significantly relieve the drunk state of the mice, but the drinking time can be effectively shortened if the vanadium-rich water is drunk for a long time before drinking, and the effect of the water on relieving the drunk state can be further enhanced if the vanadium-rich water is taken once after drinking.

In conclusion, the drinking of the vanadium-rich water once after drinking can also effectively relieve the ataxia caused by alcohol. The long-term drinking of the vanadium-rich water can effectively relieve the ataxia caused by alcohol, shorten the duration of the drunk state, and has better effect of drinking the vanadium-rich hydrolytic wine after drinking once on the basis of drinking the vanadium-rich water for a long time. Therefore, the vanadium-rich water has a good function of relieving alcoholism.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.